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Table of Contents
Summary of Contents for Diffinity Genomics RapidTip2
- Page 1 USER HANDBOOK Version B (March 2012) For PCR Purification with Polymerase Removal User Notice: For optimal purification results, sharply tap the RapidTip2 package 2–3 times on a flat surface so that the particles drop to the bottom of the tips.
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Page 2: Table Of Contents
Quality Control Technical Assistance Safety Information (Disposal) Product Specifications Introduction Diffinity Technology Principle Equipment to be Supplied by User Guidelines — Pipetting with the RapidTip2 Pipetting Examples RapidTip2 PCR Purification Protocols Quantifying DNA Recovery Troubleshooting Guide Ordering Information Page 2... -
Page 3: Kit Contents
Diffinity RapidTip2s can be used with universal or Rainin LTS style pipettes. All kits come with a user handbook. Cat Number Product Description Contents RR050-008 Diffinity RapidTip2 for 1 Pack of 8 RR050-008L* PCR Purification with RapidTip2s Polymerase Removal RR050-048... -
Page 4: Product Use Limitations
Product Use Limitations Diffinity RapidTip2s are intended for research use only. Diffinity RapidTip2 is designed for purification of 50 μl PCR solutions prior to dideoxy (BigDye) reaction for Sanger Sequencing, restriction digestion, or T-A cloning. RapidTip2 is compatible with most universal and Rainin LTS pipettors. RapidTip2 is not compatible with detergents, mineral oil, or ready-to-load style PCR master mixes that contain a density-increasing compound. -
Page 5: Quality Control
Safety Information (Disposal) Diffinity RapidTip2s may be disposed in regular lab trash. For more information, please consult the material safety data sheet (MSDS) available on request by calling Customer Service at 1-877-362-1812. Diffinity RapidTip2 Page 5... -
Page 6: Product Specifications
(less than 40–50 bp) DNA Polymerase removal up to 1:1000 dilution with (Taq, Hot-Start, High- no measurable amplification Fidelity) activity Diffinity RapidTip2 tips are designed to remove dNTPs, primers, primer-dimers, and DNA polymerase at typical PCR concentrations. Page 6 www.diffinitygenomics.com... -
Page 7: Introduction
Introduction Diffinity Genomics has developed an innovative new technology that removes undesirable impurities from PCR reactions leaving you with nothing but purified DNA! This revolutionary technology effectively removes dNTPs, primers, primer-dimers, and DNA polymerase while providing up to 90% recovery of pure DNA fragments ranging in length from 100bp to 10Kb. -
Page 8: Diffinity Technology Principle
Diffinity Technology Principle ™ The Diffinity RapidTip2 contains everything you need for PCR purification. The tip is filled with our proprietary adsorption technology, which has a differential affinity for PCR reaction components. The impurities are removed from the solution as it enters the pipette tip. -
Page 9: Equipment To Be Supplied By User
Diffinity RapidTip2 unpuri ed purification improves sequence quality. Sequence chromatograms show improved peak quality and RapidTip2 lower background in Diffinity RapidTip2 purified samples as compared to unpurified samples. Equipment to be Supplied by User • PCR Sample (50 μl ±5 μl) •... -
Page 10: Guidelines - Pipetting With The Rapidtip2
Guidelines — Pipetting with the RapidTip2 Careful pipetting is important to achieve effective purification with the RapidTip2. The following pipetting guidelines will help ensure optimal results: 1. Pre-wet the particles On the first aspirate step, aspirate approximately the first half of the sample and pause 5 seconds to ensure complete wetting of the particles before mixing. -
Page 11: Pipetting Examples
Please note that typical PCR samples are colorless — for visualization, yellow dye was used. To correct B and to improve performance, gently “flick” the pipettor downward to bring all particles and the sample back together, then proceed with mixing. Diffinity RapidTip2 Page 11... -
Page 12: Rapidtip2 Pcr Purification Protocols
RapidTip2 PCR Purification Protocols Prepare Samples: Diffinity RapidTip2 is optimized for 50 μl reaction volumes but can effectively purify samples from 45–55 μl. • For PCR volumes >55 μl, please aliquot 50 μl into a new tube for purification. • For PCR volumes <45 μl, please dilute to 50 μl. This works best with highly concentrated DNA samples (>50 ng/μl) -
Page 13: Quantifying Dna Recovery
To estimate percent recovery, analyses of the samples pre and post-purification are necessary. For this process, we recommend either a PicoGreen 2 type assay or visualization on ® agarose gel. Diffinity RapidTip2 Page 13... -
Page 14: Troubleshooting Guide
Troubleshooting Guide Problem Things to try: Low DNA Run some of the untreated and RapidTip2 purified recovery PCR on a gel to check your amplicon band intensity. Note: Purify at least 45 μl of your PCR reaction to optimize DNA recovery. -
Page 15: Ordering Information
You can place an order by: 1. Contacting one of our distributors worldwide. For a complete list, visit our website. 2. Call Diffinity Genomics Customer Service and place the order by phone: +1-877-362-1812 3. Visit our website and place an order online via the online Diffinity store: www.diffinitygenomics.com/order.asp... - Page 16 Printed in the United States Diffinity Genomics, Inc. 150 Lucius Gordon Drive, West Henrietta, NY 14586 © 2011 Diffinity Genomics, Inc. All rights reserved. Diffinity Genomics, Diffinity and RapidTip are trademarks of Diffinity Genomics, Inc. All other names may be trademarks or...
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