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Troubleshooting
Summary of Contents for Becton, Dickinson and Company BD Rhapsody Express
- Page 1 BD Rhapsody™ Express Single-Cell Analysis System Installation and Maintenance Guide For the BD Rhapsody™ Express instrument Doc ID: 76955 Rev. 2.0 08/2018 Becton, Dickinson and Company BD Biosciences 2350 Qume Drive San Jose, CA 95131 USA Tel 1.877.232.8995, prompt 2, 2 researchapplications@bd.com...
- Page 2 Trademarks are the property of their respective owners. © 2018 BD. BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. The information in this guide is subject to change without notice. BD Biosciences reserves the right to change its products and services at any time to incorporate the latest technological developments.
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Page 3: Table Of Contents
Maintaining the BD Rhapsody™ Express instrument ..... 16 Returning the BD Rhapsody Express instrument for service ....20 Maintaining the BD Rhapsody™... - Page 4 This page intentionally left blank...
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Page 5: Chapter 1: Introduction
Introduction Doc ID: 76955 Rev. 2.0... -
Page 6: About This Guide
This guide provides instructions on installing and maintaining the BD Rhapsody™ Express instrument. Intended use This guide is intended for users of the BD Rhapsody Express instrument. Read this guide prior to installing and maintaining the instruments. For intended use of the BD Rhapsody™ Single-Cell Analysis system, see the BD Rhapsody™... - Page 7 Chapter 1: Introduction Symbol Meaning Caution. Indicates the need for the user to consult the instructions for use for important cautionary information, such as warnings and precautions that cannot, for a variety of reasons, be presented on the device itself. Biological hazard.
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Page 8: Safety Data Sheets
BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide Safety data sheets Introduction This topic describes how to obtain safety data sheets (SDSs). Before handling chemicals, read and understand the SDSs. To Obtaining SDSs obtain SDSs for chemicals ordered from BD Biosciences, contact BD Biosciences technical support at researchapplications@bd.com. - Page 9 Chapter 1: Introduction Location Telephone number Location Telephone number (continued) Germany 069 22 22 25 60 Sweden 08 50 69 21 54 Greece 00800 12 75 06 Switzerland 044 58 04 373 Italy 02 36 00 36 85 United 0207 07 53 226 Kingdom Before contacting BD Biosciences, have the following information available:...
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Page 11: Chapter 2: Installation
Installation Doc ID: 76955 Rev. 2.0... -
Page 12: Bd Rhapsody™ Express Instrument Installation
BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide BD Rhapsody™ Express instrument installation Preparing the site Prepare the site for the Express instrument. See the BD Rhapsody™ Express Single-Cell Analysis System Site Preparation Guide (Doc ID: 75750). Installation Contact BD Biosciences technical support at researchapplications@bd.com. - Page 13 Chapter 2: Installation Installing the drip 1. Move the front slider to OPEN: tray and splash shield OPEN WASTE BEADS Insert drip tray here 2. Review the location of parts: Drip tray Splash guard Doc ID: 76955 Rev. 2.0...
- Page 14 BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide 3. Orient the white drip tray so that its larger compartment faces upwards and is positioned towards the interior of the instrument. The compartments of the drip tray correspond to the tube sizes above it.
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Page 15: Chapter 3: Maintenance
Maintenance Doc ID: 76955 Rev. 2.0... -
Page 16: Maintaining The Bd Rhapsody™ Express Instrument
BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide Maintaining the BD Rhapsody™ Express instrument Introduction This section describes how to maintain and decontaminate the BD Rhapsody Express instrument. General Use 10% (v/v) bleach, 70% (v/v) ethyl alcohol, or 70% (v/v) isopropyl alcohol to clean the BD Rhapsody Express instrument. - Page 17 Chapter 3: Maintenance 3. Remove the white drip tray (red oval) by lifting it slightly and pulling it out: 4. If necessary, appropriately dispose of the liquids in the drip tray. 5. Use pre-moistened cleaning wipes with 10% bleach or 70% ethyl alcohol to wipe the sides, top, and bottom of the drip tray and the tube receptacles for the Waste Collection Container (Cat.
- Page 18 BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide Cleaning the splash 1. Move the front slider to BEADS for access to the splash shield shield tab: OPEN WASTE BEADS Doc ID: 76955 Rev. 2.0...
- Page 19 Chapter 3: Maintenance 2. On the left side of the BD Rhapsody Express instrument, grasp the bottom tab, and pull out the splash shield (red oval). To avoid spills, do not tilt the tray: 3. Appropriately dispose of liquid in the splash shield.
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Page 20: Returning The Bd Rhapsody Express Instrument For Service
BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide Returning the BD Rhapsody Express instrument for service 1. Before returning the BD Rhapsody Express instrument to BD Biosciences, follow the cleaning protocol. See Maintaining the BD Rhapsody™ Express instrument (page 16). -
Page 21: Returning The Bd Rhapsody P1200M And P5000M Pipettes For Service
Chapter 3: Maintenance Returning the BD Rhapsody P1200M and P5000M pipettes for service Introduction This section describes how to calibrate and service the BD Rhapsody P1200M and P5000M pipettes. Calibrate the pipettes every 12 months to ensure accurate sample volume transfer at each step. - Page 22 BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide 4. Ship the pipettes to the appropriate Gilson Service Center: Location Shipping address Gilson Service Center 3101 Laura Lane, STE 100 Middleton, WI 53562 Europe Gilson International - France SAS Attention: OEM Department...
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Page 23: Chapter 4: Troubleshooting
Troubleshooting Doc ID: 76955 Rev. 2.0... -
Page 24: Bd Rhapsody™ Express Instrument Troubleshooting
BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide BD Rhapsody™ Express instrument troubleshooting Introduction This topic describes possible problems and recommended solutions for installing and maintaining the BD Rhapsody Express instrument. Difficulty removing the cartridge from Possible causes... - Page 25 Chapter 4: Troubleshooting Left slider is not engaging the Possible causes Recommended solutions retrieval magnet Various Move the left slider to the middle (0) position. If the Retrieval (top) magnet is not away from the cartridge tray, lift and push the Retrieval magnet away from the cartridge tray: Magnet away Push magnet...
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Page 27: Appendix A: Safety
Safety Doc ID: 76955 Rev. 2.0... -
Page 28: General Safety And Limitations
BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide General safety and limitations For complete safety information, see the BD Rhapsody™ Express Instrument Safety and Limitations Guide (Doc ID: 76918) and the BD Rhapsody™ Express Single-Cell Analysis System Instrument User Guide (Doc ID: 214063). -
Page 29: Glossary
Glossary Doc ID: 76955 Rev. 2.0... - Page 30 BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide BD Rhapsody™ Mechanical instrument used for loading the Cell Capture Beads Express instrument and cells into the BD Rhapsody™ Cartridge. BD Rhapsody™ The system includes: Express Single-Cell Analysis system •...
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