Quidel Sofia Influenza A+B Quick Reference Instructions

Study the Package Insert and User Manual
thoroughly before using Quick Reference
Instructions. This is not a complete
Package Insert.
Test Procedure
NOTE: The procedures for testing swab specimens versus liquid specimens in viral transport media are different. Read carefully.
All specimens must be at room temperature before testing.
Expiration date: Check expiration date on each individual test package or outer box before using. Do not use any test past the expiration date on the label.
Swab Test Procedure (Nasal/Nasopharyngeal)
Verify that Sofia or Dispense all of the Reagent
Sofia 2 is set to the Solution into the Reagent
desired Mode: Tube. Swirl the Reagent Tube
WALK AWAY or to dissolve its contents.
READ NOW. See
the "Using Sofia" or
"Using Sofia 2"
section for more
information.
CLIA Complexity: Waived for direct nasal swab, nasopharyngeal
swab and nasopharyngeal aspirate/wash specimens
CLIA Complexity: Moderate for nasopharyngeal swab and
nasopharyngeal aspirate/wash specimens eluted in
transport media
Place the patient swab Roll the Swab head
sample into the Reagent against the inside of the
Tube. Roll the swab at Reagent Tube as you
least 3 times while remove it. Dispose of
pressing the head the used Swab in your
against the bottom and biohazard waste.
side of the Reagent
Tube.

QUICK REFERENCE

For use with Sofia and Sofia 2.
IMPORTANT! Read instructions carefully before
beginning. The test procedure below is unique to the
Sofia Influenza A+B FIA and may differ from other Sofia
FIA procedures.
Fill the provided Small, Clear Firmly squeeze the top bulb to
empty the contents of the Small,
120 µL Fixed Volume Pipette with
Clear 120 µL Fixed Volume Pipette
patient sample from the Reagent
into the Test Cassette sample well.
Tube.
Extra liquid in the overflow bulb is
To fill the Fixed Volume Pipette
OK.
NOTE: The Fixed Volume Pipette is
with the patient sample:
designed to collect and dispense the
a) FIRMLY squeeze the top bulb.
correct amount of patient sample.
b) Still squeezing, place the Pipette
Discard the Pipette in your
tip into the sample.
biohazard waste.
c) With the Pipette tip still in the NOTE: Do not pour sample from the
Reagent Tube. Use the provided
sample, release pressure on bulb
Small, Clear 120 µL Fixed Volume
to fill the Pipette.
Pipette.
120 µL Pipette
INSTRUCTIONS
Rx only
Proceed to the
"Using Sofia" or
"Using Sofia 2"
section to complete
the test.