CORNING CoolCel Instructions For Use

Corning CoolCell
®

Quick Start

• The 12 chambers and cryogenic vials should be dry to avoid tube sticking upon freezing.
• Make sure the core (black ring) is at room temperature and seated in the bottom of the
central cavity.
• Place sample vials containing 1.0 mL of cell suspension in each well. Each well should contain
a filled vial. If freezing batch is fewer than 12 vials, fill each empty well with a Corning CoolCell
Filler Vial (2 mL vial, part number 432076) or other vial that contains equivalent volume of
freezing media.
• Note: Cell suspensions can be inserted into a room temperature CoolCell container and
successfully preserved. For optimal results, CoolCell container should be at the same temperature
as your cell suspensions.
• Check that the tubes slide in and out freely.
• Fully seat the lid on CoolCell container.
• Place CoolCell container upright into a -80°C freezer or dry ice locker. Ensure that there is at least
one inch of free space clearance around CoolCell container.
• Freeze for minimum four hours before transferring samples to archive storage.

Transferring frozen samples to archive storage

• Prepare an insulated pan or container with a one inch (2.5cm) layer of pulverized or pellet dry ice.
• Remove CoolCell container from the freezer and gently remove the lid using a gentle twisting
and rocking motion.
• Immediately invert the CoolCell container over the dry ice to recover the frozen vials. Check the
CoolCell vial chambers to ensure that all chambers are clear. If any vials have stuck, release the
vials by tapping the inverted CoolCell container on a flat surface or on the palm of your hand.

Special Notes:

• Always use dry ice to transfer cryogenic vials containing cells to permanent storage to avoid
temperature rise and cell damage. Cryogenic vial contents can rise from -80°C to over -50°C in
less than one minute if exposed to room temperature air.
• It is strongly recommended that all frozen cell cultures be checked for viability before the stock
culture is terminated.
Freezing Container
®

Instructions for Use