Troubleshooting; About Coolcell Lx; Coolcell Lx Freezing Performance; Care And Cleaning - BioCision CoolCell LX Instructions For Use

About CoolCell LX

CoolCell LX, in combination with a -80
ute that is ideal for cryo-preservation of most cultured cell lines. CoolCell LX design uses a combination of
insulation foam, radial symmetry, and a heat transfer core to regulate heat loss rather than using a large thermal
mass (alcohol-based freezing container). As a result, freezing profiles are extremely consistent from one run to
the next. Also, because of this low thermal mass, CoolCell LX will not cause a rise in local freezer temperature
and will protect nearby samples already stored in the freezer. Low thermal mass also means CoolCell LX will
rapidly return to room temperature for another freezing cycle (see fast recycle instructions above).
CoolCell® L X2 T hermal P rofile
20
0
-20
-40
-60
-80
0 50 100
Time in Minutes

Troubleshooting

Problem
Vials do not freely fit in the
chambers
Vials are stuck in CoolCell after
freezing
The lid does not fully seat

Care and cleaning

CoolCell LX is constructed of closed cell cross-linked polyethylene foam and a solid thermo-conductive core.
CoolCell LX is compatible with prolonged cryogenic temperature exposure. The foam may be cleaned by water
and mild soap. Rinse and dry thoroughly. CoolCell LX is resistant to alcohols and 10% bleach solutions. Do not
autoclave. Maximum temperature exposure: 60
BioCision, LLC
775 E. Blithedale, Suite 203, Mill Valley, CA 94941, USA, info@biocision.com, www.biocision.com
CoolCell LX Instructions, October 2012
CoolCell LX © 2012. BioCision, LLC. All rights reserved. Patents pending. BioCision, Standardizing Samples, CoolCell designations are
trademarks owned by BioCision, LLC.
PURCHASE AND/OR USE OF THIS PRODUCT SHALL CONSTITUTE ACKNOWLEDGMENT AND ACCEPTANCE OF THE
TERMS AND CONDITIONS OF THIS RESEARCH LICENSE AGREEMENT.
only the non-transferable right to use the product in internal non-commercial research ("Research Use") conducted by the buyer. Research Use does not include the right to
use the Product to manufacture products, including but not limited to cells or other biological materials, for subsequent sale; the use the Products to discover, develop, or test
any commercial product; and the re-sale of Products, either alone or in combination with other products. The sale or any other transfer or distribution to third parties of (i)
the Product, or (ii) any cells or other materials created using the Product is strictly prohibited under this Research License Agreement. Also, the use of cells or other materials
created using the Product to discover, develop, or test any commercial product is strictly prohibited under this Research License Agreement. A commercial license is required
for any commercial use of the Product, or any commercial use of cells or other materials discovered, developed, or tested using the Product. To obtain a commercial license
to use the Product, please contact BioCision.The buyer's rights to have and use the Product under the Research License Agreement will terminate immediately if the buyer en-
gages in commercial use of the Product. Upon such termination of the buyer's rights, the buyer agrees to return the Products to BioCision. This Research License Agreement
shall not be assigned or otherwise transferred by the buyer.
C freezer or dry ice locker, will provide the freezing rate of -1
O
CCLX-1
CCLX-2
CCLX-3
CCLX-4
CCLX-5
150 200
CoolCell LX is designed to fit standard screw-top 1ml and 2ml cryovials
up to 13mm in diameter and up to 55mm in height. Check that flag style
labels, if used, will not bind and hinder insertion or removal.
It is likely moisture was in the vial chambers or on the sample vial prior
to freezing. Remove the core (black ring) and tap the CoolCell LX to
dislodge vials.
Ensure that sample tubes are fully seated in chamber. The maximum
height of the tube is 55mm.
C. Avoid prolonged exposure to UV light sources.
O

CoolCell LX freezing performance

A temperature probe was placed into a 2ml cryovial con-
taining 1ml of cryopreservative and the tube was inserted
into a CoolCell LX sitting at room temperature. CoolCell
LX was then placed directly into a -80°C freezer and
the temperature rate and profile were observed over a 3
hour period. This experiment was repeated 5 consecutive
times and temperature profiles were recorded.
Conclusion: CoolCell LX showed identical cooling profiles
and phase transition over five consecutive freeze cycles.
Solution
The purchase of this product ("the Product") conveys to the buyer
C per min-
O