Bruker HYPERION User Manual
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Bruker HYPERION User Manual

Bruker HYPERION User Manual

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HYPERION
User Manual
I 24319

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Summary of Contents for Bruker HYPERION

  • Page 1 HYPERION User Manual I 24319...
  • Page 2 Brand names, registered trademarks etc. used in this manual, even if not explicitly marked as such, are not to be considered unprotected by trademarks law. They are the property of their respective owner. This manual is the original documentation for the FT-IR microscope HYPERION.

  • Page 3: Table Of Contents

    Brightness control and brightness indicator ............ 30 Microscope stages ....................31 3.5.1 Overview ........................ 31 3.5.2 Manual stage......................32 3.5.3 Focus control elements ..................33 3.5.4 Motorized stage...................... 34 3.5.5 Joystick-controlled stage movement ..............35 Bruker Optik GmbH HYPERION - User Manual...
  • Page 4 Setting contact pressure levels................89 4.12.6 Focussing ....................... 89 4.13 Measuring a sample with the ATR objective (single point measurement)..91 4.14 Measuring a sample with the ATR objective (mapping measurement) ... 95 HYPERION - User Manual Bruker Optik GmbH...
  • Page 5 Removal and installation procedure of the FPA detector ........128 Evacuating the detector dewar ................. 130 6.3.1 General Information....................130 6.3.2 Evacuating the MCT detector dewar ..............130 Replacing a damaged ATR objective crystal ........... 135 Bruker Optik GmbH HYPERION - User Manual...
  • Page 6 Motorized microscope stage ................162 Manual microscope stage.................. 162 Detectors ......................163 Heating / freezing sample stage THMS600 (Linkam)........164 Heatable sample holder ..................165 Measurement parameters .................167 Spare parts and consumables..............171 System diagram..................173 HYPERION - User Manual Bruker Optik GmbH...

  • Page 7: Safety

    ➣ Indicates a hazardous situation which, if not avoided, may result in minor or mod- erate (reversible) injury. NOTE ➣ Hazard, which could result in material damage if the appropriate safety instruc- tions are not observed. Bruker Optik GmbH HYPERION User Manual...

  • Page 8: Overview Of Possible Types Of Hazard

    Potential source of hazard: heatable sample holder and heating / freez- ing sample stage THMS600 (Linkam) Important: All warning labels on the microscope must always be kept legible. Immedi- ately replace a worn or damaged label! HYPERION User Manual Bruker Optik GmbH...

  • Page 9: Possible Hazardous Sample Materials

    Waste disposal Dispose all waste produced (chemicals, infectious and radioactively contaminated sub- stances etc.) according to the prevailing laboratory regulations. Detergents and cleaning agents must be disposed according to the special waste regulations. Bruker Optik GmbH HYPERION User Manual...

  • Page 10: Intended Use

    Use only components and accessories supplied by Bruker. For components and acces- sories made by other manufacturers and used in conjunction with the microscope, Bruker Optik GmbH does not assume any liability for safe operation and proper function- ing. WARNING...

  • Page 11: Installation

    2.5. ☞ See also the technical document Installation Requirements for IR-Microscope HYPERION 1000 / 2000 / 3000 provided by Bruker Optik GmbH in advance of the instrument delivery. The installation of the microscope includes the following actions: •...

  • Page 12: Scope Of Delivery

    • Optional OPUS software packages (e.g. OPUS/3D) including the corresponding manuals • Fluorescence option a. The HYPERION microscope is available in three different variants: HYPERION 1000, HYPERION 2000 and HYPERION 3000. For information about the available microscope variants, see section 3.1. HYPERION User Manual...

  • Page 13: Site Requirements

    • HYPERION 1000 and HYPERION 2000: 45 cm (w) x 60 cm (d) x 82 cm (h) • HYPERION 3000: 55 cm (w) x 80 cm (d) x 92 cm (h) • When preparing the installation site, take into account that the ocular at the microscope front side is easily accessi- bly.
  • Page 14 • max. pressure: 2 bar (29 psi) • controllable flow rate (In case of continuous purging, the recommended purge gas flow rate is 200 liters/hour. The purge gas flow rate should not exceed 500 liters/hour.) HYPERION User Manual Bruker Optik GmbH...

  • Page 15: Connecting The Microscope To The Power Supply

    ➣ Position the external power supply unit in such a way that it does not present a trip hazard. ➣ Do not put heavy objects on the external power supply unit. ➣ Do not place the external power supply unit on a hot surface. Bruker Optik GmbH HYPERION User Manual...

  • Page 16: Procedure

    ① cord to the mains socket outlet ② ① Important note: Connect the power cord only to a socket outlet with earthing con- tact. Make sure that the socket complies with IEC. ② HYPERION User Manual Bruker Optik GmbH...

  • Page 17: Cable Connections

    (For example purposes, it is a spectrome- ter of the TENSOR series.) Rear side of the supplied PCs Figure 2.1: Relevant connection sockets at the microscope (upper images), spectrometer (lower left image) and PC (lower right image) Bruker Optik GmbH HYPERION User Manual...
  • Page 18 ☞ Regarding the PC and the spectrometer, the description in this manual is restricted to only those sockets which are of relevance for connecting the microscope compo- nents. For detailed information about the other sockets, refer to the corresponding manual. HYPERION User Manual Bruker Optik GmbH...

  • Page 19: Connecting The Motorized Microscope Stage

    At the other cable end, the cables labeled X, Y and possibly Z are pooled together into one D-sub plug. Insert this plug in the corresponding D- sub socket at the PC. ④ ④ Bruker Optik GmbH HYPERION User Manual...

  • Page 20: Connecting The Joystick

    ① ① video camera to the S-VHS socket ② the converter box. Connect the USB cable of the con- ③ verter box to an USB port at the PC. ④ ② ③ ④ HYPERION User Manual Bruker Optik GmbH...

  • Page 21: Connecting The Mct Detector(S)

    Installation 2 2.7.5 Connecting the MCT detector(s) HYPERION 1000 and HYPERION 2000 can be equipped with at most two MCT detec- tors; HYPERION 3000 with at most one MCT detector. The required cables are included in the deliv- ① ery scope.

  • Page 22: Connecting The Fpa Detector

    Installation 2 2.7.6 Connecting the FPA detector Only HYPERION 3000 can be equipped with a FPA detector. The following cables are included in the delivery scope of the FPA detector: : • Signal cable • Trigger cable • Power supply cable (including external power supply unit) Bottom side of the FPA detector compartment Figure 2.2:...
  • Page 23 Important note: Connect the power sup- ⑤ ply cable only to a socket outlet with earthing contact Make sure that the ⑤. socket complies with IEC. Bruker Optik GmbH HYPERION User Manual...

  • Page 24: Connecting The Microscope To The Spectrometer

    CAN BUS sockets at the microscope rear side. ③ ② Note: The three CAN BUS sockets at the microscope rear side labeled ACC.CONTROL. Connect the CAN BUS cable to one of them. ③ HYPERION User Manual Bruker Optik GmbH...

  • Page 25: Connecting The Microscope To The Purge Gas Supply Line

    The required hose is not included in the standard delivery scope of the microscope. It is the operating company’s duty to provide a hose (PVC, outer diameter: 6 mm) of the required length. Make sure that the hose is rated for the indicated operating pressure. Bruker Optik GmbH HYPERION User Manual...
  • Page 26 Connect the other end of the hose to the local purge gas supply line. Important note: The connecting piece of the purge gas supply line has to be dimensioned for the connection of a hose having an outer diameter of 6 mm. HYPERION User Manual Bruker Optik GmbH...

  • Page 27: Overview

    Overview Microscope variants The HYPERION microscope is available in three different variants: HYPERION 1000 - Basic variant This variant is designed for IR spectro- scopic measurements in transmission and reflection. In addition, optional objec- tives (e.g. ATR objective, GIR objective) can be used with this microscope variant.

  • Page 28: Complete Overview - Operating Elements & Components

    Overview 3 Complete overview - operating elements & components Front view Figure 3.1: HYPERION 2000 - Front view Fig. 3.1 Component / Control element Control panel Sample stage (motorized stage) Revolving nosepiece with objective(s) LCD monitor Binocular Video camera Rotating knobs for focussing the condenser...
  • Page 29 Overview 3 Right side view Figure 3.2: HYPERION 2000 - Right side view Fig. 3.2 Component / Control element Brightness control Installation site of an optional aperture (aperture for confocal microscopy) Knife-edge aperture (manually operated type) Visible light routing control (i.e. control element for routing the light either...
  • Page 30 Overview 3 Left side view Figure 3.3: HYPERION 2000 - Left side view Fig. 3.3 Component / Control element Microscope lamp (visible light source for transmission microscopy) Opening for coupling the spectrometer or an external accessory on the left side of the microscope...

  • Page 31: Operating Panel

    Transmission mode button: to activate the transmittance mode for transmission microscopy and spectroscopic measurements in transmission. Use this mode in case of a transparent sample. Note: Activating this button automatically deactivates the reflectance mode button. Bruker Optik GmbH HYPERION User Manual...

  • Page 32: Brightness Control And Brightness Indicator

    Brightness control: Thumb wheel for adjusting the brightness intensity of the microscope illumination in a continuous manner. Icon legend to illustrate how to increase / decrease the brightness inten- sity HYPERION User Manual Bruker Optik GmbH...

  • Page 33: Microscope Stages

    Overview 3 Microscope stages 3.5.1 Overview Manual stage This type of stage is standard for the HYPERION 1000 variant. It can be moved in x-, y- and z-direction only man- ually. ☞ For information about the manual stage operation, see section 3.5.2.

  • Page 34: Manual Stage

    Note: The sample has to be placed over the hole. Rotary knob for moving the stage in z-direction: Using this knob, the stage is moved upward / downward for focussing purposes. ☞ More information about focussing, see section 3.5.3. HYPERION User Manual Bruker Optik GmbH...

  • Page 35: Focus Control Elements

    ➣ The coarse and the fine focus knobs are used for focussing in case of the following types of stage: manual stage and motorized x/y-stage. Bruker Optik GmbH HYPERION User Manual...

  • Page 36: Motorized Stage

    OPUS. For performing mapping measurements with the ATR objective, the stage option stepATRMICstage needs to be selected. For information about how to con- figure the mapping device and the imaging device, refer to the OPUS/VIDEO Manual. HYPERION User Manual Bruker Optik GmbH...

  • Page 37: Joystick-Controlled Stage Movement

    OPUS. To check whether it is activated or to activate it, proceed as fol- lows: Select in the OPUS Measure menu the Non-video-assisted mapping measurement function. On the XY Stage dialog page, make sure that the Activate joystick checkbox is activate. If not, activate it now. Bruker Optik GmbH HYPERION User Manual...

  • Page 38: Condenser

    D and D’ Centering screws: to center the condenser by moving it in x- and/or y- direction. ☞ For detailed information about how to adjust the condenser, see section 4.6. HYPERION User Manual Bruker Optik GmbH...

  • Page 39: Apertures

    • Depending on their locations, the pinhole aperture are used for adjusting the condenser and for narrowing the sample area used for analysis. By default, the HYPERION microscope is equipped with the following apertures and dia- phragms: Manual knife-edge aperture This aperture is included in the standard delivery scope.
  • Page 40 ☞ For information about how to check the signal inten- sity in the reflection mode, see section 4.7.2 ☞ For information about the optional apertures, see section 5.2. HYPERION User Manual Bruker Optik GmbH...

  • Page 41: Installation Locations Of The Apertures

    Note: It is a confocal aperture which is located in the beam path before the sample. Bruker Optik GmbH HYPERION User Manual...

  • Page 42: Manual Knife-Edge Aperture

    • rotating the complete knife-edge aperture with the previously adjusted rectangular aperture opening (rotation angle up to 360°) Which of these two functions is actually realized depends on the current position of the lever. (See B and C in fig. 3.11.) HYPERION User Manual Bruker Optik GmbH...

  • Page 43: Iris Aperture For Setting The Koehler Illumination

    This aperture (B in fig. 3.10) is used for setting the Koehler illumination in transmission mode. It is located underneath the microscope stage. This aperture is opened and closed by rotating the knurled ring. Bruker Optik GmbH HYPERION User Manual...

  • Page 44: Light Path Selector Lever

    LCD monitor at the front side of the microscope and the PC monitor using the OPUS/VIDEO software pro- gram. (Refer also to OPUS/VIDEO Manual.) Note: The microscope variant HYPERION 1000 is not equipped with a LCD monitor. HYPERION User Manual Bruker Optik GmbH...

  • Page 45: Revolving Nosepiece And Objectives

    Two vacant positions: If not used, they are covered with caps. Note: Before attaching an objective, remove the cap. 1. ATR - Attenuated Total Reflection (abgeschwächte Totalreflexion) 2. GIR - Grazing Incidence Reflection (Reflexion bei streifenden Lichteinfall) Bruker Optik GmbH HYPERION User Manual...
  • Page 46 GIR objective cannot be attached to the nosepiece if there are already two other large objectives (e.g. 15x objective and ATR objective). In this case, you have to remove one of these two large objectives first. HYPERION User Manual Bruker Optik GmbH...

  • Page 47: Binocular

    An index mark next to the interpupillary scale indicates the currently adjusted inter- pupillary distance. This information facilitates the readjustment of your individual eyepieces distance at a later point in time. Bruker Optik GmbH HYPERION User Manual...

  • Page 48: Lcd Monitor

    The following microscope variants are equipped by default with a high-resolution, color LCD monitor: HYPERION 2000 and HYPERION 3000. Figure 3.17: a) HYPERION 2000 (front side) b) Right microscope side- Operating elements of the LCD monitor Fig. 3.17 Component and explanation LCD monitor: allows for a comfortable sample viewing of the video image in real time.

  • Page 49: Detectors

    Detectors 3.12.1 Installation locations All microscope variants of the HYPERION series have two detector compartments. In the left compartment, there is the standard MCT detector (single-element detector) cov- ering a spectral range from 12,000 - 600 cm . Optionally, the right compartment can be equipped with an second detector.

  • Page 50: Types Of Detector

    Types of detector MCT detector The mid-band MCT detector is the standard detector for all microscope variants of the HYPERION series. This detector cover a spectral range from 12,000 - 600 cm In addition to the standard MCT detector, there are...

  • Page 51: Beam Path

    Overview 3 3.13 Beam path Figure 3.19: HYPERION 1000 and 2000 - Beam path in transmission mode and in reflection mode Bruker Optik GmbH HYPERION User Manual...
  • Page 52 Overview 3 Figure 3.20: HYPERION 3000 - Beam path in transmission mode and in reflection mode HYPERION User Manual Bruker Optik GmbH...
  • Page 53 Position 2: IR beam is routed to optional detector 2 (14b). Mirror: routes IR beam to detector 1 (14a). Mirror: routes IR beam to detector 2 (14b). Infrared detector IR detector or FPA detector (optional) Bruker Optik GmbH HYPERION User Manual...
  • Page 54 IR light (two polarizers). The IR polarizer (A675-P) requires the holder (A 675) including the polarizer for visible light. Optional analyzer: either for visible light only or for visible and IR light (two polarizers). HYPERION User Manual Bruker Optik GmbH...

  • Page 55: Possible Instrumental Setups

    3.14 Possible instrumental setups The HYPERION microscope is designed for the visualization and infrared spectroscopic measurements of micro samples, small sample areas as well as the examination of inhomogeneities in samples. For this purpose, the microscope needs to couple to a FT- IR spectrometer of the TENSOR or VERTEX-series.
  • Page 56 Overview 3 HYPERION User Manual Bruker Optik GmbH...

  • Page 57: Operation

    Operation General information Before you start to work with the HYPERION microscope for the first time, it is highly recommended to familiarize yourself with the operating elements of the microscope and the relevant OPUS functions. For information about ...: refer to ...:...

  • Page 58: Switching On / Off The Analysis System

    2. Switching on the spectrometer Switch on the spectrometer. (switch posi- tion: ON). The ON/OFF switch is at the spec- ① trometer rear side. ☞ Refer to the user manual of the spec- ① trometer in question. HYPERION User Manual Bruker Optik GmbH...
  • Page 59 FPA detector, see section 4.4. Switching off the analysis system ➣ Note: When switching off the analysis system, there is no strict sequence of actions you have to observe. Bruker Optik GmbH HYPERION User Manual...

  • Page 60: Preparing The Microscope For A Spectroscopic Measurement

    OVP (OPUS Validation Program). The performance can be checked by performing a PQ test (Performance Qualification) for the measurement channel with which you intend to perform spectroscopic measurements. For detailed information about this topic, refer to the OPUS Reference Manual. HYPERION User Manual Bruker Optik GmbH...

  • Page 61: Cooling The Detector

    1. The hold time indicates how long the cooling effect of the liquid nitrogen lasts. The available MCT detectors have different nominal hold times: 8, 12 and 24 hours. Bruker Optik GmbH HYPERION User Manual...

  • Page 62: Funneling Liquid Nitrogen In The Detector

    Operation 4 4.4.3 Funneling liquid nitrogen in the detector The procedure is identical for both types of detector - MCT and FPA. HYPERION 1000 and HYPERION 2000 Filling hole for stan- Filling hole for optional dard MCT detector MCT detector Open the corresponding filling hole by removing the plug and insert the funnel.
  • Page 63 12 hours. For this reason it is advisable to switch off the FPA detector if it is not in use. ➣ Note: The ON/POFF switch of the FPA detector is at the bottom side of the right detector comportment. Bruker Optik GmbH HYPERION User Manual...

  • Page 64: Resetting The Motorized Stage

    OPUS software or by the joystick. Otherwise there is a potential risk of crushing the fingers. HYPERION User Manual Bruker Optik GmbH...

  • Page 65: Checking And Correcting The Condenser Setting

    ③ operating panel. Move the pinhole aperture in the beam path by rotating the thumb wheel in the arrow ④ direction of the small circle. ④ Bruker Optik GmbH HYPERION User Manual...
  • Page 66 When the check has revealed that the condenser is set opti- mally, rotate the thumb wheel in the arrow direction of ⑥ the big circle to move the pin- hole aperture out of the beam path again. ⑥ HYPERION User Manual Bruker Optik GmbH...
  • Page 67 Attention: After having set the con- denser correctly, do not forget to move the pinhole aperture out of the beam path again by rotating the thumb wheel in the arrow direction of the big cir- ③ cle. ③ Bruker Optik GmbH HYPERION User Manual...

  • Page 68: Checking The Ir Signal Intensity

    For information about the available standard experiment files, appendix B. Click in the Measurement dialog on the Check Signal tab. Make sure that the Interferogram option button is activated. Interferogram Interferogram option button (activated) HYPERION User Manual Bruker Optik GmbH...
  • Page 69 4) with the amplitude value for the transmission mode stated in the supplied OVP test protocol (see image in step 5). The supplied test protocol Hyperion OVP Supplementary Sheet documents the result of a factory-performed signal check using your analysis system. The test has been performed with all optics compo- nents being optimally adjusted.

  • Page 70: Procedure In Reflection Mode

    View through the binocular and adjust the ⑤ brightness using corresponding thumb wheel until the image is well- ⑤ illuminated. Focus on the mirror surface. Note: In case the mirror surface is free of scratches, focus on a mirror edge. HYPERION User Manual Bruker Optik GmbH...
  • Page 71 ➣ The precious procedure depends on whether you work with a validated or a non- validated analysis system and wether the HYPERION microscope is equipped with a manual knife-edge aperture (standard) or a motorized knife-edge aperture (option). Non-validated analysis system and...
  • Page 72 For information about the available standard experiment files, appendix B. Click in the Measurement dialog on the Check Signal tab. Make sure that the Interferogram option button is activated. Interferogram Interferogram option button (activated) HYPERION User Manual Bruker Optik GmbH...
  • Page 73 10) with the amplitude value for the reflection mode stated in the supplied OVP test protocol (see image in step 11). The supplied test protocol Hyperion OVP Supplementary Sheet documents the result of a factory-performed signal check using your analysis system. The test has been performed with all optics compo- nents being optimally adjusted.

  • Page 74: Procedure With Atr Objective

    Bring the ATR crystal in the measuring mode position (i.e. lower position). ☞ See section 4.12.4 ATR crystal in the lower position Set the contact pressure level 1 at the ATR objective. ☞ See section 4.12.5. Contact pressure level display HYPERION User Manual Bruker Optik GmbH...
  • Page 75 For information about the available standard experiment files, appendix B. Click in the Measurement dialog on the Check Signal tab. Make sure that the Interferogram option button is activated. Interferogram Interferogram option button (activated) Bruker Optik GmbH HYPERION User Manual...
  • Page 76 9) with the amplitude value with ATR objective stated in the supplied OVP test protocol (see image in step 10). The supplied test protocol Hyperion OVP Supplementary Sheet documents the result of a factory-performed signal check using your analysis system. The test has been performed with all optics compo- nents being optimally adjusted.

  • Page 77: Checking The Detector Saturation

    (See fig. 4.1.) The spectrum in fig. 4.1 results from a normal detector saturation. Enlarged section This option but- ton needs to be activated. Figure 4.1: Spectrum acquired with a normally saturated detector Bruker Optik GmbH HYPERION User Manual...
  • Page 78 This solution variant involves the potential risk of forgetting to undo this aperture setting afterwards again.) • only in case of HYPERION 3000: insert a beam attenuator in the optical adapta- tion box between microscope and spectrometer HYPERION User Manual...

  • Page 79: Viewing The Sample And Selecting The Measurement Area

    Viewing the sample and selecting the measurement area With the HYPERION microscope, you can view the sample through the binocular, on the LCD monitor at the microscope front side (except for HYPERION 1000) and in the OPUS video view on the PC monitor.

  • Page 80: Viewing And Measuring A Sample In Transmission

    Caution: Do not look through the binocu- OPUS video wizard lar when the brightness is set at maxi- mum. Temporary eye irritations may The currently set brightness is dis- occur. played by the brightness indicator (A in fig. 3.5). HYPERION User Manual Bruker Optik GmbH...
  • Page 81 Manual knife-edge aperture (D in not intended for spectroscopic analysis. fig. 3.10 and fig. 3.11) Motorized knife-edge aperture (F in fig. 3.10) This aperture is operated by the corresponding OPUS functions. ☞ See OPUS/VIDEO Manual. Bruker Optik GmbH HYPERION User Manual...
  • Page 82 Iris aperture for setting the Koehler illu- the Koehler illumination in transmission mination (B in fig. 3.10) (B in fig. 18) again. See step 8. Knurled ring underneath the condenser HYPERION User Manual Bruker Optik GmbH...
  • Page 83 ☞ See section 4.6. 21. Start the sample measurement. corresponding OPUS function ☞ See OPUS/VIDEO Manual. ➣ Afterwards, OPUS calculates automatically the result spectrum of the sample and displays the sample spectrum in the OPUS spectrum window. Bruker Optik GmbH HYPERION User Manual...

  • Page 84: Viewing And Measuring A Sample In Reflection

    Caution: Do not look through the bin- video wizard ocular when the brightness is set at maximum. Temporary eye irritations The currently set brightness is displayed may occur. by the brightness indicator (A in fig. 3.5). HYPERION User Manual Bruker Optik GmbH...
  • Page 85 ☞ See OPUS/VIDEO Manual. Note: In case of a manual knife-edge aperture, it is advisable to take a snap- shot in order to be able to reconstruct the aperture setting later, if required. Bruker Optik GmbH HYPERION User Manual...
  • Page 86 16. Start background measurement. corresponding OPUS function ☞ See OPUS/VIDEO Manual. 17. Activate viewing mode. either the VIS button on the operating panel (B in fig. 3.4) or in the OPUS video wizard ☞ See OPUS/VIDEO Manual. HYPERION User Manual Bruker Optik GmbH...
  • Page 87 ☞ See OPUS/VIDEO Manual. 20. Start the sample measurement. corresponding OPUS function ☞ See OPUS/VIDEO Manual. ➣ Afterwards, OPUS calculates automatically the result spectrum of the sample and displays the sample spectrum in the OPUS spectrum window. Bruker Optik GmbH HYPERION User Manual...

  • Page 88: Atr Objective - Important Operating Notes

    Before each measurement, check whether the ATR crystal is clean (i.e. free of any parti- cles from the previous sample). Residuals of the previous sample may lead to falsified results. Clean the ATR crystal using a lint-free cloth and a suitable solvent (e.g. ethanol, isopro- pyl), if required. HYPERION User Manual Bruker Optik GmbH...

  • Page 89: Attaching The Atr Objective At The Revolving Nosepiece

    ③ Connect the cable of the ATR objec- tive at the corresponding socket ③ the left microscope side. To remove the ATR objective from the nosepiece, first disconnect the cable than detach the objective. Bruker Optik GmbH HYPERION User Manual...

  • Page 90: Setting The Viewing Mode / Measuring Mode At The Atr Objective

    Then, release the rocker switch again. Attention: When setting the measur- ing mode, take care that the ATR crys- tal tip does not hit the stage. ③ Potential risk of damaging the ATR crystal! HYPERION User Manual Bruker Optik GmbH...

  • Page 91: Setting Contact Pressure Levels

    Focus on the sample by moving the stage slowly upwards. ➣ Attention: When moving the stage upwards, take care the ATR crystal tip does not hit the stage. Potential risk of damaging the ATR crystal! Bruker Optik GmbH HYPERION User Manual...
  • Page 92 Red: ATR crystal is not in focus posi- tion. Green: ATR crystal is in focus position. Note: In case the ATR crystal is in the viewing mode position, the In-focus LED does not light at all. HYPERION User Manual Bruker Optik GmbH...

  • Page 93: Measuring A Sample With The Atr Objective (Single Point Measurement)

    Manual stage: Rotary knobs (A and B in tion to find a sample area which is of fig. 3.6) interest to you. Motorized stage: Joystick and corre- sponding stage control functions in OPUS ☞ See OPUS/VIDEO Manual. Bruker Optik GmbH HYPERION User Manual...
  • Page 94 When the ATR crys- tal has reached the focus position, the ☞ See OPUS/VIDEO Manual. In-focus LED turns from red to green ☞ See also section 4.12.6. for a short moment. Then it goes off. HYPERION User Manual Bruker Optik GmbH...
  • Page 95 Manual stage: Rotary knobs (A and B in intend to analyze spectroscopically by fig. 3.6) moving the stage in x- and/or y-direc- Motorized stage: Joystick and corre- tion. sponding stage control functions in OPUS ☞ See OPUS/VIDEO Manual. Bruker Optik GmbH HYPERION User Manual...
  • Page 96 Then it goes off. 23. Start the sample measurement. OPUS software ☞ See OPUS/VIDEO Manual. ➣ Afterwards, OPUS calculates automatically the result spectrum of the sample and displays the sample spectrum in the OPUS spectrum window. HYPERION User Manual Bruker Optik GmbH...

  • Page 97: Measuring A Sample With The Atr Objective (Mapping Measurement)

    • Objective: 20x ATR • Electronic options: Autofocus • Motorized X/Y stage: lstepATRMICstage ☞ For detailed information about how to configure the mapping device and the imag- ing device, refer to the OPUS/VIDEO Manual. Bruker Optik GmbH HYPERION User Manual...
  • Page 98 When the ATR crystal has reached the focus position, the In- focus LED turns from red to green for a short moment. Then it goes off. 4. Start the background measurement. OPUS software ☞ See OPUS/VIDEO Manual. HYPERION User Manual Bruker Optik GmbH...
  • Page 99 3.2.! disabled! 8.3. Move the stage upwards until the distance between sample and ATR crys- tal tip is less than 2 mm. Bruker Optik GmbH HYPERION User Manual...
  • Page 100 The mapping measurement result is saved automatically in a 3D file. ☞ For detailed information about this OPUS file type including the available OPUS functions regarding editing, manipulation and evaluation, refer to the OPUS/3D Manual. HYPERION User Manual Bruker Optik GmbH...

  • Page 101: Gir Objective - Important Operating Notes

    GIR objective again. (See section 4.15.5.) When you screw the GIR objective in the same thread of the revolving nosepiece again, normally a readjustment might not be necessary. 1. GIR objective - Grazing Incidence Reflection Angle Objective Bruker Optik GmbH HYPERION User Manual...

  • Page 102: Design Of The Mirror Optics

    (See section 4.15.3.) The following figure shows the arrangement of the mirrors inside the GIR objective. plane mirrors ellipsoidal mirror arranged in parallel Figure 4.3: GIR objective - Design of the mirror optics HYPERION User Manual Bruker Optik GmbH...

  • Page 103: Setting The Viewing Mode (Vis) Or Measuring Mode (Gir)

    Afterwards, the IR beam is redirected through the objective into the micro- scope and impinges on the detector. Viewing mode (VIS) Measuring mode (GIR) Figure 4.5: Beam path in VIS mode and in GIR mode Bruker Optik GmbH HYPERION User Manual...

  • Page 104: Images Generated By The Gir Objective

    Image of the measurement spot in GIR mode - primary spot in GIR mode - primary image and secondary image lie image and secondary image do one upon the other not lie one upon the other HYPERION User Manual Bruker Optik GmbH...

  • Page 105: Checking The Alignment Of The Gir Objective

    VIS mode - Image of a vertically aligned mirror edge Set the mirror optics of the GIR objective to the measurement mode (GIR). Be care- ful not to displace the mirror lying on the stage unintentionally! Bruker Optik GmbH HYPERION User Manual...

  • Page 106: Realigning The Gir Objective

    1. fastening screws for the bot- tom part of the objective hous- ing and 2. knobs for setting the VIS mode or GIR mode Figure 4.9: Removing the bottom part of the objective housing HYPERION User Manual Bruker Optik GmbH...
  • Page 107 12. Rotate the lower part of the GIR objective slightly to the left or to the right (fig. 4.11) until the displayed image shows a vertically aligned mirror edge. See fig. 4.8a. Be careful not to displace the mirror lying on the stage unintentionally! Bruker Optik GmbH HYPERION User Manual...
  • Page 108 In doing so, do not misalign the mirror optics again! 15. Remove the GIR objective from the revolving nosepiece. 16. Fit the bottom part of the housing on the objective and fasten it using the four slot- ted-head screws. See fig. 4.9. HYPERION User Manual Bruker Optik GmbH...

  • Page 109: Measuring A Sample With The Gir Objective

    GIR objec- tive might get damaged. 6. Set the mirror optics of the GIR GIR objective (fig. 4.4) objective to the measurement mode ☞ See section 4.15.3. (GIR). Bruker Optik GmbH HYPERION User Manual...
  • Page 110 (VIS). ☞ See section 4.15.3. 12. Microscope: Activate the viewing either the VIS button on the operating mode. panel (B in fig. 3.4) or in the OPUS video wizard ☞ See OPUS/VIDEO Manual. HYPERION User Manual Bruker Optik GmbH...
  • Page 111 Afterwards, OPUS calculates automatically the result spectrum of the sample and displays the sample spectrum in the OPUS spectrum window. Due to the low signal intensity, it is recommended to perform the measurement with a large number of scans (e.g. 500 or 1000 Scans). Bruker Optik GmbH HYPERION User Manual...

  • Page 112: Purging The Microscope

    ☞ For information about the purge gas supply requirements, see section 2.5. ☞ For information about how to connect the microscope to the purge gas supply line, see section 2.8. 1. The purge housing design ensures a reduction of environmental influences on the sample area. HYPERION User Manual Bruker Optik GmbH...

  • Page 113: Options And Accessories

    Options and Accessories Optional objectives 5.1.1 Overview For the HYPERION microscope, the following optional objectives are available: 4x objective (glass objective) This objective is for visual sample viewing only. It is not qualified for IR spectroscopic measurements! Reason: Glass absorbs mid-infrared light.

  • Page 114: Additional Information About The Gir Objective

    1. The aluminum mirror is included in the standard delivery scope. Note: For measuring the background spec- trum use the gold mirror which is included in the standard delivery scope as well. HYPERION User Manual Bruker Optik GmbH...

  • Page 115: Optional Apertures

    Options and Accessories 5 Optional apertures 5.2.1 Overview For the HYPERION microscope, the following optional apertures are available: Motorized knife-edge aperture The optional motorized knife-edge aperture can be installed in place of the manually-operated knife-edge aperture (standard). The motorized knife-edge aperture is available only with transparent knife edges made of glass.

  • Page 116: Installation Location Of The Exchangeable Apertures

    42.0 75.0 100.0 1.80 50.0 90.0 120.0 2.10 58.0 10.5 140.0 2.50 69.0 125.0 170.0 3.00 83.0 150.0 200.0 3.75 104.0 187.5 250.0 5.2.2 Installation location of the exchangeable apertures Figure 5.2: Aperture locations HYPERION User Manual Bruker Optik GmbH...
  • Page 117 A and B (in fig. 5.2) are interchangeable with each other. However, the apertures which can be installed in place C (in fig. 5.2) are not interchangeable with the apertures which can be installed at place A and B (in fig. 5.2). Bruker Optik GmbH HYPERION User Manual...

  • Page 118: Optional Microscope Stages And Sample Holders

    For example: It holds spherical and other irregularly shaped samples It permits tilting the sample or leveling an oblique-angled sample. It holds and stretches the sample (e.g. fibers, polymer films etc.). HYPERION User Manual Bruker Optik GmbH...

  • Page 119: Heating/Freezing Stage Thms 600 (Linkam)

    OPUS function, refer to the OPUS/ Dynamic PRO Manual. manually by means of the temperature ☞ Refer to the accompanying manual. controller computer-controlled by means of the ☞ Refer to the accompanying manual. temperature control software LinkSys Bruker Optik GmbH HYPERION User Manual...

  • Page 120: Safety Notes

    For measurements with the heatable sample stage, Bruker recommends using only the 15x objective. The sample is placed in the round opening of the sample holder. This opening has a diameter of 13 mm which is the standard pellet size.

  • Page 121: Safety Notes

    Temperature information is stored together with the spectra if the temperature has been controlled by the OPUS software. Measurements during temperature ramps are only possible with the OPUS measurement function Protein Dynamics (optional software package OPUS/PRO). Bruker Optik GmbH HYPERION User Manual...

  • Page 122: Polarizer

    The polarizer material is zinc selenide (ZnSe). Zinc selenide is toxic. During normal operation and in an undamaged state, the polarizer material does not pose any health hazard. However, if the material breaks because of mechanical impact, be extremely careful and observe the following safety instructions: HYPERION User Manual Bruker Optik GmbH...

  • Page 123: Installation Location

    For IR spectroscopic measurements, usually one IR polarizer is sufficient. The IR polar- izer can be inserted into any slot (A, B or C in fig. 5.6). The polarizer holders are interchangeable. They can be inserted into any slot (A, B or C in fir. 5.6). Bruker Optik GmbH HYPERION User Manual...

  • Page 124: Performing Ir Spectroscopic Analyses Using Polarized Light

    (or possibly three) independent infrared spectra, depending on the polarizing direction of the IR light in relation to the optical axis of the material. Measurements of IR dichroism can be used, for example, to determine the orientation of polymer fibers. HYPERION User Manual Bruker Optik GmbH...

  • Page 125: External Sample Cabinet - Imac

    In most cases, a beam attenuator is required for measurements with the IMAC. The beam attenuator has to be inserted in the corresponding slot of the optical adaptation box between spectrometer and HYPERION 3000. (See fig. 5.8.) ☞ For detailed information about the external sample cabinet IMAC, refer to the accompanying documentation.

  • Page 126: Compression Cells

    Note: This box is only required for coupling a HYPERION 3000 microscope to a FT-IR Beam attenuator spectrometer. Figure 5.8: HYPERION 3000 coupled to IMAC and FT IR spectrometer Compression cells The following compression cells are available: • Micro compression cell Diamond EX’Press - A565-S1 •...

  • Page 127: Repair And Maintenance

    Adhere strictly to the described procedures and observe all relevant safety precautions. Otherwise, personal injury and/or microscope damage can be the result. In this case, Bruker does not assume any liability. Repair and maintenance works which are not described in this manual have to be performed by Bruker service personnel only.
  • Page 128 Ground cable (yellow-green) Step 4 Disconnect the cable of the temper- ature sensor from the detector. Cable temperature sensor (white brown) Step 5 Disconnect the data cable from the detector. Data cable (gray) HYPERION User Manual Bruker Optik GmbH...
  • Page 129 To reinstall the MCT detector, perform the above steps in reverse order: Insert the MCT detector carefully into the detector compartment. Fasten the detector base plate. Reconnect all cables to the MCT detector. Reinstall the detector compartment housing. Switch on the spectrometer. Bruker Optik GmbH HYPERION User Manual...

  • Page 130: Removal And Installation Procedure Of The Fpa Detector

    Note: For loosening the two front screws, use an Allen wrench (5 mm). The back screw is factory-equipped with a kind of screw driver. Step 5 Remove the FPA detector from the detector compartment. HYPERION User Manual Bruker Optik GmbH...
  • Page 131 Insert the FPA detector into the detector compartment. Make sure that the detector base plate snaps into the guide rail. Fasten the detector base plate. Reconnect all cables to the FPA detector. Reinstall the detector compartment housing. Switch on the FPA detector. Bruker Optik GmbH HYPERION User Manual...

  • Page 132: Evacuating The Detector Dewar

    2x flexible metal hoses The above listed evacuating equipment is NOT included in the standard delivery scope of the MCT detector. If desired, Bruker offers suitable evacuating equipment (part num- ber S105-V2, D126 and I10290). Alternatively, Bruker also offers the service of evacuat- ing the MCT detector dewar (part number SD128).
  • Page 133 Remove the cap from the connection nozzle of the detector. (See fig. 6.2a.) Pull the adapter knob (G in fig. 6.3) to the open position and loosen the coupling nut (A in fig. 6.3). Bruker Optik GmbH HYPERION User Manual...
  • Page 134 We recommend an evacuation time of at least 3 days to allow for generating an opti- mal vacuum inside the dewar. The final pressure in the detector dewar should be less than 10 mbar. HYPERION User Manual Bruker Optik GmbH...
  • Page 135 (e.g. when you close the evacuation valve) you can perform mea- surements with this MCT detector for the moment but after a relatively short period of time you have to repeat the detector evacuation. closed open Figure 6.3: Adapter - Cross section Bruker Optik GmbH HYPERION User Manual...
  • Page 136 Components of the connecting adapter Coupling nut O-ring retainer O-ring Threaded rod (to remove the valve closure of the detector dewar) Connecting piece for vacuum pump (OD = 9,7mm) Washer and O-ring packing Knob HYPERION User Manual Bruker Optik GmbH...

  • Page 137: Replacing A Damaged Atr Objective Crystal

    (See section 4.12.4.) Put the supplied mirror on the microscope stage. Position the mirror below the ATR crystal. Move the stage carefully upwards until the distance between ATR crystal tip and stage is approx. 2 mm. Bruker Optik GmbH HYPERION User Manual...

  • Page 138: Replacing The Atr Crystal Assembly

    As the very small fastening elements (screws, washers, springs and pins) may easily get lost during the replacement of the crystal assembly, a complete set of mounting hard- ware as spare parts is included in the delivery scope of the replacement crystal as well. HYPERION User Manual Bruker Optik GmbH...
  • Page 139 (Note: This hex wrench is included in the delivery scope of the ATR crystal.) Slotted-head screws at the outer ring Step 1 Loosen and remove the three slotted- head screws at the outer ring using a small flat tip screwdriver. Bruker Optik GmbH HYPERION User Manual...
  • Page 140 Make sure that the two recesses of the inner ring (i.e. recesses for inserting the springs) are aligned with the corre- sponding recesses of the outer ring. HYPERION User Manual Bruker Optik GmbH...
  • Page 141 Tighten the screws only loosely. Do not yet tighten them firmly! Note: Do not tighten these screws firmly before you have aligned the ATR crystal centrically. For information about how to align the ATR crystal centrically, see section 6.4.5. Bruker Optik GmbH HYPERION User Manual...
  • Page 142 ☞ For information about how to attach the ATR objective to the revolving nosepiece and how to connect the ATR objective to the microscope, see section 4.12.3. HYPERION User Manual Bruker Optik GmbH...

  • Page 143: Checking The Atr Crystal Position For Centrality

    ART crystal is in the center position. In this case, no realignment in horizontal direction is required. Otherwise, you have to align the ATR crystal centrically. Figure 6.6: a) ATR crystal in center position b) ATR crystal not aligned centrically Bruker Optik GmbH HYPERION User Manual...

  • Page 144: Aligning The Atr Crystal Centrically

    ATR objective bottom side (fig. 6.8). Otherwise, repeat step 1 and step 2 above. When tightening firmly the three slotted-head screws at the ATR objective bottom side, be careful not to dis- place the crystal assembly again! HYPERION User Manual Bruker Optik GmbH...

  • Page 145: Checking The Height Setting Of The Atr Crystal

    (See fig. 6.9b.) 12. Move the microscope stage slowly upwards until the plastic ring pushes the ATR crystal in the focus position. 1. The plastic ring is included in the delivery scope of the ATR objective. Bruker Optik GmbH HYPERION User Manual...

  • Page 146: Correcting The Height Setting Of The Atr Crystal

    Actuate the rocker switch and pull the outer casing of the ATR objective downwards as far as it will go (i.e. until it snaps into place at pressure level 5). (See section 4.12.5.) HYPERION User Manual Bruker Optik GmbH...
  • Page 147 ATR crystal by slightly rotating the adjusting screw using the hex wrench. Cor- rect the setting until the maximum signal intensity (amplitude value) is displayed in OPUS. 1. The plastic ring is included in the delivery scope of the ATR objective. Bruker Optik GmbH HYPERION User Manual...
  • Page 148 Repair and Maintenance 6 HYPERION User Manual Bruker Optik GmbH...

  • Page 149: Troubleshooting

    Important note: Due to complex instrumental setup, it is recommended to proceed sys- tematically. First try to find out which instrument (FT IR spectrometer or HYPERION microscope) has cause the problem. For this reason, consult also the User Manual of the FT IR spectrometer in question for troubleshooting.

  • Page 150: Problem - Possible Cause - Solution

    VIS polarizer in the beam path. ☞ See section 5.4. There is not any objective in the beam path. Swing an objective in the beam path. Contact the Bruker service. Microscope lamp (LED) is defect. ☞ See section 1.5. Switch on the microscope.

  • Page 151: No Video Image Is Displayed On The Lcd Monitor And/Or In Opus

    No video image is displayed on the LCD monitor and/or in OPUS Possible cause Solution Pull out the light path selector lever completely. Visible light is routed to the binocular. ☞ See section 3.8. Bruker Optik GmbH HYPERION User Manual...

  • Page 152: No Ir Signal Is Detected Or The Detected Ir Signal Intensity Is Too Low

    IR signal in transmission or reflection, the wrong mode is activated. Note: The currently activated mode is indicated by a green lit LED at the corresponding mode button on the operating panel. HYPERION User Manual Bruker Optik GmbH...
  • Page 153 4.8. • only in case of HYPERION 3000: inserting a beam attenuator in the optical adaptation box between microscope and spectrometer (See fig. 7.1.) Transmission mode: Remove the sample from the microscope stage.
  • Page 154 Sample does not reflect light at all or has a stage in x- and/or y-direction. very poor light reflecting capacity. If you do not succeed in finding a reflective sample area, the sample is not suited sample viewing in the reflection mode. HYPERION User Manual Bruker Optik GmbH...

  • Page 155: Ir Signal Check In Opus Shows An Unusual Spectrum Curve Shape

    4.8. • only in case of HYPERION 3000: inserting a beam attenuator in the optical adaptation box between microscope and spectrometer (See fig. 7.1.) Bruker Optik GmbH HYPERION User Manual...

  • Page 156: Problems Regarding The Motorized Microscope Stage

    See section 2.7.3. Activate this function in OPUS Joystick control fails. by selecting in the Measure- OPUS, function ment menu the Motorized enabling a joystick-controlled Stage Control function and is deactivated. activating the Activate joystick checkbox. HYPERION User Manual Bruker Optik GmbH...

  • Page 157: Problems Regarding The Atr Objective

    Connect it cor- ceed in find the focus position nected or not connected prop- rectly. because the In-focus LED erly to the microscope. ☞ See section 4.12.3. does not indicate a focus posi- tion at all. Bruker Optik GmbH HYPERION User Manual...
  • Page 158 ☞ See section 4.12.5. Clean the ATR crystal. • ATR crystal is dirty. ☞ See section 4.12.2. HYPERION User Manual Bruker Optik GmbH...

  • Page 159: Problems Regarding Mapping Measurements

    Imaging device. ☞ For information about how to setup the Note: Autofocus is an electronic option. imaging device, refer to the OPUS/VIDEO Manual. Bruker Optik GmbH HYPERION User Manual...

  • Page 160: Problems Regarding The Fpa Detector

    See fig. 7.1. Check the cable for correct connection. Con- The power supply cable and/or the signal cable nect it correctly. is/are not connected at all or not connected properly. ☞ See section 2.7.6. HYPERION User Manual Bruker Optik GmbH...
  • Page 161 ☞ See section 5.4. Detector oversaturation (even at high wavenumbers; IMAC) Possible cause Solution Insert the beam attenuator in the optical adap- IR signal intensity is too high. tation box. See fig. 7.1. Bruker Optik GmbH HYPERION User Manual...
  • Page 162 Note: This box is only required for coupling a HYPERION 3000 micro- scope to a FT IR spectrometer. Figure 7.1: HYPERION 3000 with FPA detector (coupled to a FT IR spectrometer by means of an optical adaptation box) HYPERION User Manual...

  • Page 163: Specifications

    Pollution degree: 2 according to EN 61010-1 or IEC 60664-1 Protection class: I according to IEC 61140 Objectives Objective Objective Visible Numerical Working magnification magnification aperture distance* (in mm) 4x objective (VIS) 40x objective (VIS) 400x 0.65 15x objective (IR) 150x Bruker Optik GmbH HYPERION User Manual...

  • Page 164: Motorized Microscope Stage

    100 mm x 80 mm Positioning accuracy ± 0.1 µm at measurement point Repeatability better than1 µm Manual microscope stage Parameter Specification max. travel range in x- and y-direction 50 mm x 75 mm HYPERION User Manual Bruker Optik GmbH...

  • Page 165: Detectors

    Specifications A Detectors Detectors for HYPERION 1000 and HYPERION 2000 The detectors listed in the table below are single-element detectors. Mode of cooling / Detector Spectral range Detection sensitivity nominal hold time Standard MCT detector with liquid N 12,000 - 600 cm D*>2x10**10cm Hz...

  • Page 166: Heating / Freezing Sample Stage Thms600 (Linkam)

    Specifications A FPA detectors for HYPERION 3000 The FPA detectors listed in the table below are multi-element detectors. Detector Number of Spectral range Mode of cooling / detector elements Nominal hold time photovoltaic MCT 16,384 elements with liquid N 4,500 - 900 cm...

  • Page 167: Heatable Sample Holder

    Dimension of the heatable sample holder 75 mm x 50 mm x 10 mm Dimension of the temperature controller 260 mm x 150 mm x 270 mm Cable length ~ 50 cm Power supply requirements 230V, 100W, 50/60Hz Bruker Optik GmbH HYPERION User Manual...
  • Page 168 Specifications A HYPERION User Manual Bruker Optik GmbH...

  • Page 169: B Measurement Parameters

    (e.g. regarding data acquisition, the optical components to be used etc.) and save them in an experiment file (’*’.xpm). Depending on the combination of HYPERION variant and measurement technique, OPUS includes experiment files with appropriate default parameter settings. These files...
  • Page 170 Figure A.1: OPUS dialog Video assisted Measurements - Dialog page Optics parameter 1. Mapping measurements are only possible if the microscope is equipped with a motorized sample stage. 2. The FPA detector is only available for the microscope variant HYPERION 3000. HYPERION User Manual...
  • Page 171 ☞ The parameter settings and values on the other dialog pages (Advanced, Acquisi- tion and FT) depend on the FT IR spectrometer to which the HYPERION micro- scope is coupled. For information about the default settings of these parameters, refer to the User Manual of the FT IR spectrometer in question. Consult also the OPUS Reference Manual.
  • Page 172 Measurement parameters B HYPERION User Manual Bruker Optik GmbH...

  • Page 173: C Spare Parts And Consumables

    Dimensions: 30 mm x 24 mm x 3 mm Pack of 5 mirrors A697-R10 Ge crystal, replacement ATR crystal assembly for ATR objective in case of HYPERION 1000 and HYPERION 2000 A697-R10B Ge crystal, replacement ATR crystal assembly for ATR objective in case of HYPERION 3000...
  • Page 174 Spare parts and consumables C HYPERION User Manual Bruker Optik GmbH...

  • Page 175: D System Diagram

    System diagram Bruker Optik GmbH HYPERION User Manual...
  • Page 176 System diagram D HYPERION User Manual Bruker Optik GmbH...
  • Page 177 Number of detector elements Beam attenuator 76 ......57 ON / OFF switch Beam path ..........49 ......128 Removing / reinstalling .......49 HYPERION 1000 and 2000 ........164 Spectral range ........50 HYPERION 3000 Binocular ....26 Brightness Bruker Optik GmbH HYPERION - User Manual...
  • Page 178 Heating/freezing stage THMS600 (Linkam) ........130 Nominal hold time ..........117 ......125 Removing / reinstalling ........164 Specifications Measurement parameters .......167 HYPERION 1000 ....25 Measuring mode HYPERION 2000 ...25 ..........88 ATR objective HYPERION 3000 ...25 .........101 GIR objective ..........29...
  • Page 179 Viewing and measuring a sample Transmission mode button ....29 Video camera ........26 Video camera port ........52 Viewing mode ..........88 ATR objective .........101 GIR objective ..........29 Microscope VIS button ......29 VIS polarizer ..120 Bruker Optik GmbH HYPERION - User Manual...
  • Page 180 HYPERION - User Manual Bruker Optik GmbH...

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