Page 2
Brand names, registered trademarks etc. used in this manual, even if not explicitly marked as such, are not to be considered unprotected by trademarks law. They are the property of their respective owner. This manual is the original documentation for the FT-IR microscope HYPERION.
Brightness control and brightness indicator ............ 30 Microscope stages ....................31 3.5.1 Overview ........................ 31 3.5.2 Manual stage......................32 3.5.3 Focus control elements ..................33 3.5.4 Motorized stage...................... 34 3.5.5 Joystick-controlled stage movement ..............35 Bruker Optik GmbH HYPERION - User Manual...
Page 4
Setting contact pressure levels................89 4.12.6 Focussing ....................... 89 4.13 Measuring a sample with the ATR objective (single point measurement)..91 4.14 Measuring a sample with the ATR objective (mapping measurement) ... 95 HYPERION - User Manual Bruker Optik GmbH...
Page 5
Removal and installation procedure of the FPA detector ........128 Evacuating the detector dewar ................. 130 6.3.1 General Information....................130 6.3.2 Evacuating the MCT detector dewar ..............130 Replacing a damaged ATR objective crystal ........... 135 Bruker Optik GmbH HYPERION - User Manual...
Page 6
Motorized microscope stage ................162 Manual microscope stage.................. 162 Detectors ......................163 Heating / freezing sample stage THMS600 (Linkam)........164 Heatable sample holder ..................165 Measurement parameters .................167 Spare parts and consumables..............171 System diagram..................173 HYPERION - User Manual Bruker Optik GmbH...
➣ Indicates a hazardous situation which, if not avoided, may result in minor or mod- erate (reversible) injury. NOTE ➣ Hazard, which could result in material damage if the appropriate safety instruc- tions are not observed. Bruker Optik GmbH HYPERION User Manual...
Potential source of hazard: heatable sample holder and heating / freez- ing sample stage THMS600 (Linkam) Important: All warning labels on the microscope must always be kept legible. Immedi- ately replace a worn or damaged label! HYPERION User Manual Bruker Optik GmbH...
Waste disposal Dispose all waste produced (chemicals, infectious and radioactively contaminated sub- stances etc.) according to the prevailing laboratory regulations. Detergents and cleaning agents must be disposed according to the special waste regulations. Bruker Optik GmbH HYPERION User Manual...
Use only components and accessories supplied by Bruker. For components and acces- sories made by other manufacturers and used in conjunction with the microscope, Bruker Optik GmbH does not assume any liability for safe operation and proper function- ing. WARNING...
2.5. ☞ See also the technical document Installation Requirements for IR-Microscope HYPERION 1000 / 2000 / 3000 provided by Bruker Optik GmbH in advance of the instrument delivery. The installation of the microscope includes the following actions: •...
• Optional OPUS software packages (e.g. OPUS/3D) including the corresponding manuals • Fluorescence option a. The HYPERION microscope is available in three different variants: HYPERION 1000, HYPERION 2000 and HYPERION 3000. For information about the available microscope variants, see section 3.1. HYPERION User Manual...
• HYPERION 1000 and HYPERION 2000: 45 cm (w) x 60 cm (d) x 82 cm (h) • HYPERION 3000: 55 cm (w) x 80 cm (d) x 92 cm (h) • When preparing the installation site, take into account that the ocular at the microscope front side is easily accessi- bly.
Page 14
• max. pressure: 2 bar (29 psi) • controllable flow rate (In case of continuous purging, the recommended purge gas flow rate is 200 liters/hour. The purge gas flow rate should not exceed 500 liters/hour.) HYPERION User Manual Bruker Optik GmbH...
➣ Position the external power supply unit in such a way that it does not present a trip hazard. ➣ Do not put heavy objects on the external power supply unit. ➣ Do not place the external power supply unit on a hot surface. Bruker Optik GmbH HYPERION User Manual...
① cord to the mains socket outlet ② ① Important note: Connect the power cord only to a socket outlet with earthing con- tact. Make sure that the socket complies with IEC. ② HYPERION User Manual Bruker Optik GmbH...
(For example purposes, it is a spectrome- ter of the TENSOR series.) Rear side of the supplied PCs Figure 2.1: Relevant connection sockets at the microscope (upper images), spectrometer (lower left image) and PC (lower right image) Bruker Optik GmbH HYPERION User Manual...
Page 18
☞ Regarding the PC and the spectrometer, the description in this manual is restricted to only those sockets which are of relevance for connecting the microscope compo- nents. For detailed information about the other sockets, refer to the corresponding manual. HYPERION User Manual Bruker Optik GmbH...
At the other cable end, the cables labeled X, Y and possibly Z are pooled together into one D-sub plug. Insert this plug in the corresponding D- sub socket at the PC. ④ ④ Bruker Optik GmbH HYPERION User Manual...
① ① video camera to the S-VHS socket ② the converter box. Connect the USB cable of the con- ③ verter box to an USB port at the PC. ④ ② ③ ④ HYPERION User Manual Bruker Optik GmbH...
Installation 2 2.7.5 Connecting the MCT detector(s) HYPERION 1000 and HYPERION 2000 can be equipped with at most two MCT detec- tors; HYPERION 3000 with at most one MCT detector. The required cables are included in the deliv- ① ery scope.
Installation 2 2.7.6 Connecting the FPA detector Only HYPERION 3000 can be equipped with a FPA detector. The following cables are included in the delivery scope of the FPA detector: : • Signal cable • Trigger cable • Power supply cable (including external power supply unit) Bottom side of the FPA detector compartment Figure 2.2:...
Page 23
Important note: Connect the power sup- ⑤ ply cable only to a socket outlet with earthing contact Make sure that the ⑤. socket complies with IEC. Bruker Optik GmbH HYPERION User Manual...
CAN BUS sockets at the microscope rear side. ③ ② Note: The three CAN BUS sockets at the microscope rear side labeled ACC.CONTROL. Connect the CAN BUS cable to one of them. ③ HYPERION User Manual Bruker Optik GmbH...
The required hose is not included in the standard delivery scope of the microscope. It is the operating company’s duty to provide a hose (PVC, outer diameter: 6 mm) of the required length. Make sure that the hose is rated for the indicated operating pressure. Bruker Optik GmbH HYPERION User Manual...
Page 26
Connect the other end of the hose to the local purge gas supply line. Important note: The connecting piece of the purge gas supply line has to be dimensioned for the connection of a hose having an outer diameter of 6 mm. HYPERION User Manual Bruker Optik GmbH...
Overview Microscope variants The HYPERION microscope is available in three different variants: HYPERION 1000 - Basic variant This variant is designed for IR spectro- scopic measurements in transmission and reflection. In addition, optional objec- tives (e.g. ATR objective, GIR objective) can be used with this microscope variant.
Overview 3 Complete overview - operating elements & components Front view Figure 3.1: HYPERION 2000 - Front view Fig. 3.1 Component / Control element Control panel Sample stage (motorized stage) Revolving nosepiece with objective(s) LCD monitor Binocular Video camera Rotating knobs for focussing the condenser...
Page 29
Overview 3 Right side view Figure 3.2: HYPERION 2000 - Right side view Fig. 3.2 Component / Control element Brightness control Installation site of an optional aperture (aperture for confocal microscopy) Knife-edge aperture (manually operated type) Visible light routing control (i.e. control element for routing the light either...
Page 30
Overview 3 Left side view Figure 3.3: HYPERION 2000 - Left side view Fig. 3.3 Component / Control element Microscope lamp (visible light source for transmission microscopy) Opening for coupling the spectrometer or an external accessory on the left side of the microscope...
Transmission mode button: to activate the transmittance mode for transmission microscopy and spectroscopic measurements in transmission. Use this mode in case of a transparent sample. Note: Activating this button automatically deactivates the reflectance mode button. Bruker Optik GmbH HYPERION User Manual...
Brightness control: Thumb wheel for adjusting the brightness intensity of the microscope illumination in a continuous manner. Icon legend to illustrate how to increase / decrease the brightness inten- sity HYPERION User Manual Bruker Optik GmbH...
Overview 3 Microscope stages 3.5.1 Overview Manual stage This type of stage is standard for the HYPERION 1000 variant. It can be moved in x-, y- and z-direction only man- ually. ☞ For information about the manual stage operation, see section 3.5.2.
Note: The sample has to be placed over the hole. Rotary knob for moving the stage in z-direction: Using this knob, the stage is moved upward / downward for focussing purposes. ☞ More information about focussing, see section 3.5.3. HYPERION User Manual Bruker Optik GmbH...
➣ The coarse and the fine focus knobs are used for focussing in case of the following types of stage: manual stage and motorized x/y-stage. Bruker Optik GmbH HYPERION User Manual...
OPUS. For performing mapping measurements with the ATR objective, the stage option stepATRMICstage needs to be selected. For information about how to con- figure the mapping device and the imaging device, refer to the OPUS/VIDEO Manual. HYPERION User Manual Bruker Optik GmbH...
OPUS. To check whether it is activated or to activate it, proceed as fol- lows: Select in the OPUS Measure menu the Non-video-assisted mapping measurement function. On the XY Stage dialog page, make sure that the Activate joystick checkbox is activate. If not, activate it now. Bruker Optik GmbH HYPERION User Manual...
D and D’ Centering screws: to center the condenser by moving it in x- and/or y- direction. ☞ For detailed information about how to adjust the condenser, see section 4.6. HYPERION User Manual Bruker Optik GmbH...
• Depending on their locations, the pinhole aperture are used for adjusting the condenser and for narrowing the sample area used for analysis. By default, the HYPERION microscope is equipped with the following apertures and dia- phragms: Manual knife-edge aperture This aperture is included in the standard delivery scope.
Page 40
☞ For information about how to check the signal inten- sity in the reflection mode, see section 4.7.2 ☞ For information about the optional apertures, see section 5.2. HYPERION User Manual Bruker Optik GmbH...
• rotating the complete knife-edge aperture with the previously adjusted rectangular aperture opening (rotation angle up to 360°) Which of these two functions is actually realized depends on the current position of the lever. (See B and C in fig. 3.11.) HYPERION User Manual Bruker Optik GmbH...
This aperture (B in fig. 3.10) is used for setting the Koehler illumination in transmission mode. It is located underneath the microscope stage. This aperture is opened and closed by rotating the knurled ring. Bruker Optik GmbH HYPERION User Manual...
LCD monitor at the front side of the microscope and the PC monitor using the OPUS/VIDEO software pro- gram. (Refer also to OPUS/VIDEO Manual.) Note: The microscope variant HYPERION 1000 is not equipped with a LCD monitor. HYPERION User Manual Bruker Optik GmbH...
Two vacant positions: If not used, they are covered with caps. Note: Before attaching an objective, remove the cap. 1. ATR - Attenuated Total Reflection (abgeschwächte Totalreflexion) 2. GIR - Grazing Incidence Reflection (Reflexion bei streifenden Lichteinfall) Bruker Optik GmbH HYPERION User Manual...
Page 46
GIR objective cannot be attached to the nosepiece if there are already two other large objectives (e.g. 15x objective and ATR objective). In this case, you have to remove one of these two large objectives first. HYPERION User Manual Bruker Optik GmbH...
An index mark next to the interpupillary scale indicates the currently adjusted inter- pupillary distance. This information facilitates the readjustment of your individual eyepieces distance at a later point in time. Bruker Optik GmbH HYPERION User Manual...
The following microscope variants are equipped by default with a high-resolution, color LCD monitor: HYPERION 2000 and HYPERION 3000. Figure 3.17: a) HYPERION 2000 (front side) b) Right microscope side- Operating elements of the LCD monitor Fig. 3.17 Component and explanation LCD monitor: allows for a comfortable sample viewing of the video image in real time.
Detectors 3.12.1 Installation locations All microscope variants of the HYPERION series have two detector compartments. In the left compartment, there is the standard MCT detector (single-element detector) cov- ering a spectral range from 12,000 - 600 cm . Optionally, the right compartment can be equipped with an second detector.
Types of detector MCT detector The mid-band MCT detector is the standard detector for all microscope variants of the HYPERION series. This detector cover a spectral range from 12,000 - 600 cm In addition to the standard MCT detector, there are...
Overview 3 3.13 Beam path Figure 3.19: HYPERION 1000 and 2000 - Beam path in transmission mode and in reflection mode Bruker Optik GmbH HYPERION User Manual...
Page 52
Overview 3 Figure 3.20: HYPERION 3000 - Beam path in transmission mode and in reflection mode HYPERION User Manual Bruker Optik GmbH...
Page 53
Position 2: IR beam is routed to optional detector 2 (14b). Mirror: routes IR beam to detector 1 (14a). Mirror: routes IR beam to detector 2 (14b). Infrared detector IR detector or FPA detector (optional) Bruker Optik GmbH HYPERION User Manual...
Page 54
IR light (two polarizers). The IR polarizer (A675-P) requires the holder (A 675) including the polarizer for visible light. Optional analyzer: either for visible light only or for visible and IR light (two polarizers). HYPERION User Manual Bruker Optik GmbH...
3.14 Possible instrumental setups The HYPERION microscope is designed for the visualization and infrared spectroscopic measurements of micro samples, small sample areas as well as the examination of inhomogeneities in samples. For this purpose, the microscope needs to couple to a FT- IR spectrometer of the TENSOR or VERTEX-series.
Page 56
Overview 3 HYPERION User Manual Bruker Optik GmbH...
Operation General information Before you start to work with the HYPERION microscope for the first time, it is highly recommended to familiarize yourself with the operating elements of the microscope and the relevant OPUS functions. For information about ...: refer to ...:...
2. Switching on the spectrometer Switch on the spectrometer. (switch posi- tion: ON). The ON/OFF switch is at the spec- ① trometer rear side. ☞ Refer to the user manual of the spec- ① trometer in question. HYPERION User Manual Bruker Optik GmbH...
Page 59
FPA detector, see section 4.4. Switching off the analysis system ➣ Note: When switching off the analysis system, there is no strict sequence of actions you have to observe. Bruker Optik GmbH HYPERION User Manual...
OVP (OPUS Validation Program). The performance can be checked by performing a PQ test (Performance Qualification) for the measurement channel with which you intend to perform spectroscopic measurements. For detailed information about this topic, refer to the OPUS Reference Manual. HYPERION User Manual Bruker Optik GmbH...
1. The hold time indicates how long the cooling effect of the liquid nitrogen lasts. The available MCT detectors have different nominal hold times: 8, 12 and 24 hours. Bruker Optik GmbH HYPERION User Manual...
Operation 4 4.4.3 Funneling liquid nitrogen in the detector The procedure is identical for both types of detector - MCT and FPA. HYPERION 1000 and HYPERION 2000 Filling hole for stan- Filling hole for optional dard MCT detector MCT detector Open the corresponding filling hole by removing the plug and insert the funnel.
Page 63
12 hours. For this reason it is advisable to switch off the FPA detector if it is not in use. ➣ Note: The ON/POFF switch of the FPA detector is at the bottom side of the right detector comportment. Bruker Optik GmbH HYPERION User Manual...
③ operating panel. Move the pinhole aperture in the beam path by rotating the thumb wheel in the arrow ④ direction of the small circle. ④ Bruker Optik GmbH HYPERION User Manual...
Page 66
When the check has revealed that the condenser is set opti- mally, rotate the thumb wheel in the arrow direction of ⑥ the big circle to move the pin- hole aperture out of the beam path again. ⑥ HYPERION User Manual Bruker Optik GmbH...
Page 67
Attention: After having set the con- denser correctly, do not forget to move the pinhole aperture out of the beam path again by rotating the thumb wheel in the arrow direction of the big cir- ③ cle. ③ Bruker Optik GmbH HYPERION User Manual...
For information about the available standard experiment files, appendix B. Click in the Measurement dialog on the Check Signal tab. Make sure that the Interferogram option button is activated. Interferogram Interferogram option button (activated) HYPERION User Manual Bruker Optik GmbH...
Page 69
4) with the amplitude value for the transmission mode stated in the supplied OVP test protocol (see image in step 5). The supplied test protocol Hyperion OVP Supplementary Sheet documents the result of a factory-performed signal check using your analysis system. The test has been performed with all optics compo- nents being optimally adjusted.
View through the binocular and adjust the ⑤ brightness using corresponding thumb wheel until the image is well- ⑤ illuminated. Focus on the mirror surface. Note: In case the mirror surface is free of scratches, focus on a mirror edge. HYPERION User Manual Bruker Optik GmbH...
Page 71
➣ The precious procedure depends on whether you work with a validated or a non- validated analysis system and wether the HYPERION microscope is equipped with a manual knife-edge aperture (standard) or a motorized knife-edge aperture (option). Non-validated analysis system and...
Page 72
For information about the available standard experiment files, appendix B. Click in the Measurement dialog on the Check Signal tab. Make sure that the Interferogram option button is activated. Interferogram Interferogram option button (activated) HYPERION User Manual Bruker Optik GmbH...
Page 73
10) with the amplitude value for the reflection mode stated in the supplied OVP test protocol (see image in step 11). The supplied test protocol Hyperion OVP Supplementary Sheet documents the result of a factory-performed signal check using your analysis system. The test has been performed with all optics compo- nents being optimally adjusted.
Bring the ATR crystal in the measuring mode position (i.e. lower position). ☞ See section 4.12.4 ATR crystal in the lower position Set the contact pressure level 1 at the ATR objective. ☞ See section 4.12.5. Contact pressure level display HYPERION User Manual Bruker Optik GmbH...
Page 75
For information about the available standard experiment files, appendix B. Click in the Measurement dialog on the Check Signal tab. Make sure that the Interferogram option button is activated. Interferogram Interferogram option button (activated) Bruker Optik GmbH HYPERION User Manual...
Page 76
9) with the amplitude value with ATR objective stated in the supplied OVP test protocol (see image in step 10). The supplied test protocol Hyperion OVP Supplementary Sheet documents the result of a factory-performed signal check using your analysis system. The test has been performed with all optics compo- nents being optimally adjusted.
(See fig. 4.1.) The spectrum in fig. 4.1 results from a normal detector saturation. Enlarged section This option but- ton needs to be activated. Figure 4.1: Spectrum acquired with a normally saturated detector Bruker Optik GmbH HYPERION User Manual...
Page 78
This solution variant involves the potential risk of forgetting to undo this aperture setting afterwards again.) • only in case of HYPERION 3000: insert a beam attenuator in the optical adapta- tion box between microscope and spectrometer HYPERION User Manual...
Viewing the sample and selecting the measurement area With the HYPERION microscope, you can view the sample through the binocular, on the LCD monitor at the microscope front side (except for HYPERION 1000) and in the OPUS video view on the PC monitor.
Caution: Do not look through the binocu- OPUS video wizard lar when the brightness is set at maxi- mum. Temporary eye irritations may The currently set brightness is dis- occur. played by the brightness indicator (A in fig. 3.5). HYPERION User Manual Bruker Optik GmbH...
Page 81
Manual knife-edge aperture (D in not intended for spectroscopic analysis. fig. 3.10 and fig. 3.11) Motorized knife-edge aperture (F in fig. 3.10) This aperture is operated by the corresponding OPUS functions. ☞ See OPUS/VIDEO Manual. Bruker Optik GmbH HYPERION User Manual...
Page 82
Iris aperture for setting the Koehler illu- the Koehler illumination in transmission mination (B in fig. 3.10) (B in fig. 18) again. See step 8. Knurled ring underneath the condenser HYPERION User Manual Bruker Optik GmbH...
Page 83
☞ See section 4.6. 21. Start the sample measurement. corresponding OPUS function ☞ See OPUS/VIDEO Manual. ➣ Afterwards, OPUS calculates automatically the result spectrum of the sample and displays the sample spectrum in the OPUS spectrum window. Bruker Optik GmbH HYPERION User Manual...
Caution: Do not look through the bin- video wizard ocular when the brightness is set at maximum. Temporary eye irritations The currently set brightness is displayed may occur. by the brightness indicator (A in fig. 3.5). HYPERION User Manual Bruker Optik GmbH...
Page 85
☞ See OPUS/VIDEO Manual. Note: In case of a manual knife-edge aperture, it is advisable to take a snap- shot in order to be able to reconstruct the aperture setting later, if required. Bruker Optik GmbH HYPERION User Manual...
Page 86
16. Start background measurement. corresponding OPUS function ☞ See OPUS/VIDEO Manual. 17. Activate viewing mode. either the VIS button on the operating panel (B in fig. 3.4) or in the OPUS video wizard ☞ See OPUS/VIDEO Manual. HYPERION User Manual Bruker Optik GmbH...
Page 87
☞ See OPUS/VIDEO Manual. 20. Start the sample measurement. corresponding OPUS function ☞ See OPUS/VIDEO Manual. ➣ Afterwards, OPUS calculates automatically the result spectrum of the sample and displays the sample spectrum in the OPUS spectrum window. Bruker Optik GmbH HYPERION User Manual...
Before each measurement, check whether the ATR crystal is clean (i.e. free of any parti- cles from the previous sample). Residuals of the previous sample may lead to falsified results. Clean the ATR crystal using a lint-free cloth and a suitable solvent (e.g. ethanol, isopro- pyl), if required. HYPERION User Manual Bruker Optik GmbH...
③ Connect the cable of the ATR objec- tive at the corresponding socket ③ the left microscope side. To remove the ATR objective from the nosepiece, first disconnect the cable than detach the objective. Bruker Optik GmbH HYPERION User Manual...
Then, release the rocker switch again. Attention: When setting the measur- ing mode, take care that the ATR crys- tal tip does not hit the stage. ③ Potential risk of damaging the ATR crystal! HYPERION User Manual Bruker Optik GmbH...
Focus on the sample by moving the stage slowly upwards. ➣ Attention: When moving the stage upwards, take care the ATR crystal tip does not hit the stage. Potential risk of damaging the ATR crystal! Bruker Optik GmbH HYPERION User Manual...
Page 92
Red: ATR crystal is not in focus posi- tion. Green: ATR crystal is in focus position. Note: In case the ATR crystal is in the viewing mode position, the In-focus LED does not light at all. HYPERION User Manual Bruker Optik GmbH...
Manual stage: Rotary knobs (A and B in tion to find a sample area which is of fig. 3.6) interest to you. Motorized stage: Joystick and corre- sponding stage control functions in OPUS ☞ See OPUS/VIDEO Manual. Bruker Optik GmbH HYPERION User Manual...
Page 94
When the ATR crys- tal has reached the focus position, the ☞ See OPUS/VIDEO Manual. In-focus LED turns from red to green ☞ See also section 4.12.6. for a short moment. Then it goes off. HYPERION User Manual Bruker Optik GmbH...
Page 95
Manual stage: Rotary knobs (A and B in intend to analyze spectroscopically by fig. 3.6) moving the stage in x- and/or y-direc- Motorized stage: Joystick and corre- tion. sponding stage control functions in OPUS ☞ See OPUS/VIDEO Manual. Bruker Optik GmbH HYPERION User Manual...
Page 96
Then it goes off. 23. Start the sample measurement. OPUS software ☞ See OPUS/VIDEO Manual. ➣ Afterwards, OPUS calculates automatically the result spectrum of the sample and displays the sample spectrum in the OPUS spectrum window. HYPERION User Manual Bruker Optik GmbH...
• Objective: 20x ATR • Electronic options: Autofocus • Motorized X/Y stage: lstepATRMICstage ☞ For detailed information about how to configure the mapping device and the imag- ing device, refer to the OPUS/VIDEO Manual. Bruker Optik GmbH HYPERION User Manual...
Page 98
When the ATR crystal has reached the focus position, the In- focus LED turns from red to green for a short moment. Then it goes off. 4. Start the background measurement. OPUS software ☞ See OPUS/VIDEO Manual. HYPERION User Manual Bruker Optik GmbH...
Page 99
3.2.! disabled! 8.3. Move the stage upwards until the distance between sample and ATR crys- tal tip is less than 2 mm. Bruker Optik GmbH HYPERION User Manual...
Page 100
The mapping measurement result is saved automatically in a 3D file. ☞ For detailed information about this OPUS file type including the available OPUS functions regarding editing, manipulation and evaluation, refer to the OPUS/3D Manual. HYPERION User Manual Bruker Optik GmbH...
GIR objective again. (See section 4.15.5.) When you screw the GIR objective in the same thread of the revolving nosepiece again, normally a readjustment might not be necessary. 1. GIR objective - Grazing Incidence Reflection Angle Objective Bruker Optik GmbH HYPERION User Manual...
(See section 4.15.3.) The following figure shows the arrangement of the mirrors inside the GIR objective. plane mirrors ellipsoidal mirror arranged in parallel Figure 4.3: GIR objective - Design of the mirror optics HYPERION User Manual Bruker Optik GmbH...
Afterwards, the IR beam is redirected through the objective into the micro- scope and impinges on the detector. Viewing mode (VIS) Measuring mode (GIR) Figure 4.5: Beam path in VIS mode and in GIR mode Bruker Optik GmbH HYPERION User Manual...
Image of the measurement spot in GIR mode - primary spot in GIR mode - primary image and secondary image lie image and secondary image do one upon the other not lie one upon the other HYPERION User Manual Bruker Optik GmbH...
VIS mode - Image of a vertically aligned mirror edge Set the mirror optics of the GIR objective to the measurement mode (GIR). Be care- ful not to displace the mirror lying on the stage unintentionally! Bruker Optik GmbH HYPERION User Manual...
1. fastening screws for the bot- tom part of the objective hous- ing and 2. knobs for setting the VIS mode or GIR mode Figure 4.9: Removing the bottom part of the objective housing HYPERION User Manual Bruker Optik GmbH...
Page 107
12. Rotate the lower part of the GIR objective slightly to the left or to the right (fig. 4.11) until the displayed image shows a vertically aligned mirror edge. See fig. 4.8a. Be careful not to displace the mirror lying on the stage unintentionally! Bruker Optik GmbH HYPERION User Manual...
Page 108
In doing so, do not misalign the mirror optics again! 15. Remove the GIR objective from the revolving nosepiece. 16. Fit the bottom part of the housing on the objective and fasten it using the four slot- ted-head screws. See fig. 4.9. HYPERION User Manual Bruker Optik GmbH...
GIR objec- tive might get damaged. 6. Set the mirror optics of the GIR GIR objective (fig. 4.4) objective to the measurement mode ☞ See section 4.15.3. (GIR). Bruker Optik GmbH HYPERION User Manual...
Page 110
(VIS). ☞ See section 4.15.3. 12. Microscope: Activate the viewing either the VIS button on the operating mode. panel (B in fig. 3.4) or in the OPUS video wizard ☞ See OPUS/VIDEO Manual. HYPERION User Manual Bruker Optik GmbH...
Page 111
Afterwards, OPUS calculates automatically the result spectrum of the sample and displays the sample spectrum in the OPUS spectrum window. Due to the low signal intensity, it is recommended to perform the measurement with a large number of scans (e.g. 500 or 1000 Scans). Bruker Optik GmbH HYPERION User Manual...
☞ For information about the purge gas supply requirements, see section 2.5. ☞ For information about how to connect the microscope to the purge gas supply line, see section 2.8. 1. The purge housing design ensures a reduction of environmental influences on the sample area. HYPERION User Manual Bruker Optik GmbH...
Options and Accessories Optional objectives 5.1.1 Overview For the HYPERION microscope, the following optional objectives are available: 4x objective (glass objective) This objective is for visual sample viewing only. It is not qualified for IR spectroscopic measurements! Reason: Glass absorbs mid-infrared light.
1. The aluminum mirror is included in the standard delivery scope. Note: For measuring the background spec- trum use the gold mirror which is included in the standard delivery scope as well. HYPERION User Manual Bruker Optik GmbH...
Options and Accessories 5 Optional apertures 5.2.1 Overview For the HYPERION microscope, the following optional apertures are available: Motorized knife-edge aperture The optional motorized knife-edge aperture can be installed in place of the manually-operated knife-edge aperture (standard). The motorized knife-edge aperture is available only with transparent knife edges made of glass.
42.0 75.0 100.0 1.80 50.0 90.0 120.0 2.10 58.0 10.5 140.0 2.50 69.0 125.0 170.0 3.00 83.0 150.0 200.0 3.75 104.0 187.5 250.0 5.2.2 Installation location of the exchangeable apertures Figure 5.2: Aperture locations HYPERION User Manual Bruker Optik GmbH...
Page 117
A and B (in fig. 5.2) are interchangeable with each other. However, the apertures which can be installed in place C (in fig. 5.2) are not interchangeable with the apertures which can be installed at place A and B (in fig. 5.2). Bruker Optik GmbH HYPERION User Manual...
For example: It holds spherical and other irregularly shaped samples It permits tilting the sample or leveling an oblique-angled sample. It holds and stretches the sample (e.g. fibers, polymer films etc.). HYPERION User Manual Bruker Optik GmbH...
OPUS function, refer to the OPUS/ Dynamic PRO Manual. manually by means of the temperature ☞ Refer to the accompanying manual. controller computer-controlled by means of the ☞ Refer to the accompanying manual. temperature control software LinkSys Bruker Optik GmbH HYPERION User Manual...
For measurements with the heatable sample stage, Bruker recommends using only the 15x objective. The sample is placed in the round opening of the sample holder. This opening has a diameter of 13 mm which is the standard pellet size.
Temperature information is stored together with the spectra if the temperature has been controlled by the OPUS software. Measurements during temperature ramps are only possible with the OPUS measurement function Protein Dynamics (optional software package OPUS/PRO). Bruker Optik GmbH HYPERION User Manual...
The polarizer material is zinc selenide (ZnSe). Zinc selenide is toxic. During normal operation and in an undamaged state, the polarizer material does not pose any health hazard. However, if the material breaks because of mechanical impact, be extremely careful and observe the following safety instructions: HYPERION User Manual Bruker Optik GmbH...
For IR spectroscopic measurements, usually one IR polarizer is sufficient. The IR polar- izer can be inserted into any slot (A, B or C in fig. 5.6). The polarizer holders are interchangeable. They can be inserted into any slot (A, B or C in fir. 5.6). Bruker Optik GmbH HYPERION User Manual...
(or possibly three) independent infrared spectra, depending on the polarizing direction of the IR light in relation to the optical axis of the material. Measurements of IR dichroism can be used, for example, to determine the orientation of polymer fibers. HYPERION User Manual Bruker Optik GmbH...
In most cases, a beam attenuator is required for measurements with the IMAC. The beam attenuator has to be inserted in the corresponding slot of the optical adaptation box between spectrometer and HYPERION 3000. (See fig. 5.8.) ☞ For detailed information about the external sample cabinet IMAC, refer to the accompanying documentation.
Note: This box is only required for coupling a HYPERION 3000 microscope to a FT-IR Beam attenuator spectrometer. Figure 5.8: HYPERION 3000 coupled to IMAC and FT IR spectrometer Compression cells The following compression cells are available: • Micro compression cell Diamond EX’Press - A565-S1 •...
Adhere strictly to the described procedures and observe all relevant safety precautions. Otherwise, personal injury and/or microscope damage can be the result. In this case, Bruker does not assume any liability. Repair and maintenance works which are not described in this manual have to be performed by Bruker service personnel only.
Page 128
Ground cable (yellow-green) Step 4 Disconnect the cable of the temper- ature sensor from the detector. Cable temperature sensor (white brown) Step 5 Disconnect the data cable from the detector. Data cable (gray) HYPERION User Manual Bruker Optik GmbH...
Page 129
To reinstall the MCT detector, perform the above steps in reverse order: Insert the MCT detector carefully into the detector compartment. Fasten the detector base plate. Reconnect all cables to the MCT detector. Reinstall the detector compartment housing. Switch on the spectrometer. Bruker Optik GmbH HYPERION User Manual...
Note: For loosening the two front screws, use an Allen wrench (5 mm). The back screw is factory-equipped with a kind of screw driver. Step 5 Remove the FPA detector from the detector compartment. HYPERION User Manual Bruker Optik GmbH...
Page 131
Insert the FPA detector into the detector compartment. Make sure that the detector base plate snaps into the guide rail. Fasten the detector base plate. Reconnect all cables to the FPA detector. Reinstall the detector compartment housing. Switch on the FPA detector. Bruker Optik GmbH HYPERION User Manual...
2x flexible metal hoses The above listed evacuating equipment is NOT included in the standard delivery scope of the MCT detector. If desired, Bruker offers suitable evacuating equipment (part num- ber S105-V2, D126 and I10290). Alternatively, Bruker also offers the service of evacuat- ing the MCT detector dewar (part number SD128).
Page 133
Remove the cap from the connection nozzle of the detector. (See fig. 6.2a.) Pull the adapter knob (G in fig. 6.3) to the open position and loosen the coupling nut (A in fig. 6.3). Bruker Optik GmbH HYPERION User Manual...
Page 134
We recommend an evacuation time of at least 3 days to allow for generating an opti- mal vacuum inside the dewar. The final pressure in the detector dewar should be less than 10 mbar. HYPERION User Manual Bruker Optik GmbH...
Page 135
(e.g. when you close the evacuation valve) you can perform mea- surements with this MCT detector for the moment but after a relatively short period of time you have to repeat the detector evacuation. closed open Figure 6.3: Adapter - Cross section Bruker Optik GmbH HYPERION User Manual...
Page 136
Components of the connecting adapter Coupling nut O-ring retainer O-ring Threaded rod (to remove the valve closure of the detector dewar) Connecting piece for vacuum pump (OD = 9,7mm) Washer and O-ring packing Knob HYPERION User Manual Bruker Optik GmbH...
(See section 4.12.4.) Put the supplied mirror on the microscope stage. Position the mirror below the ATR crystal. Move the stage carefully upwards until the distance between ATR crystal tip and stage is approx. 2 mm. Bruker Optik GmbH HYPERION User Manual...
As the very small fastening elements (screws, washers, springs and pins) may easily get lost during the replacement of the crystal assembly, a complete set of mounting hard- ware as spare parts is included in the delivery scope of the replacement crystal as well. HYPERION User Manual Bruker Optik GmbH...
Page 139
(Note: This hex wrench is included in the delivery scope of the ATR crystal.) Slotted-head screws at the outer ring Step 1 Loosen and remove the three slotted- head screws at the outer ring using a small flat tip screwdriver. Bruker Optik GmbH HYPERION User Manual...
Page 140
Make sure that the two recesses of the inner ring (i.e. recesses for inserting the springs) are aligned with the corre- sponding recesses of the outer ring. HYPERION User Manual Bruker Optik GmbH...
Page 141
Tighten the screws only loosely. Do not yet tighten them firmly! Note: Do not tighten these screws firmly before you have aligned the ATR crystal centrically. For information about how to align the ATR crystal centrically, see section 6.4.5. Bruker Optik GmbH HYPERION User Manual...
Page 142
☞ For information about how to attach the ATR objective to the revolving nosepiece and how to connect the ATR objective to the microscope, see section 4.12.3. HYPERION User Manual Bruker Optik GmbH...
ART crystal is in the center position. In this case, no realignment in horizontal direction is required. Otherwise, you have to align the ATR crystal centrically. Figure 6.6: a) ATR crystal in center position b) ATR crystal not aligned centrically Bruker Optik GmbH HYPERION User Manual...
ATR objective bottom side (fig. 6.8). Otherwise, repeat step 1 and step 2 above. When tightening firmly the three slotted-head screws at the ATR objective bottom side, be careful not to dis- place the crystal assembly again! HYPERION User Manual Bruker Optik GmbH...
(See fig. 6.9b.) 12. Move the microscope stage slowly upwards until the plastic ring pushes the ATR crystal in the focus position. 1. The plastic ring is included in the delivery scope of the ATR objective. Bruker Optik GmbH HYPERION User Manual...
Actuate the rocker switch and pull the outer casing of the ATR objective downwards as far as it will go (i.e. until it snaps into place at pressure level 5). (See section 4.12.5.) HYPERION User Manual Bruker Optik GmbH...
Page 147
ATR crystal by slightly rotating the adjusting screw using the hex wrench. Cor- rect the setting until the maximum signal intensity (amplitude value) is displayed in OPUS. 1. The plastic ring is included in the delivery scope of the ATR objective. Bruker Optik GmbH HYPERION User Manual...
Page 148
Repair and Maintenance 6 HYPERION User Manual Bruker Optik GmbH...
Important note: Due to complex instrumental setup, it is recommended to proceed sys- tematically. First try to find out which instrument (FT IR spectrometer or HYPERION microscope) has cause the problem. For this reason, consult also the User Manual of the FT IR spectrometer in question for troubleshooting.
VIS polarizer in the beam path. ☞ See section 5.4. There is not any objective in the beam path. Swing an objective in the beam path. Contact the Bruker service. Microscope lamp (LED) is defect. ☞ See section 1.5. Switch on the microscope.
No video image is displayed on the LCD monitor and/or in OPUS Possible cause Solution Pull out the light path selector lever completely. Visible light is routed to the binocular. ☞ See section 3.8. Bruker Optik GmbH HYPERION User Manual...
IR signal in transmission or reflection, the wrong mode is activated. Note: The currently activated mode is indicated by a green lit LED at the corresponding mode button on the operating panel. HYPERION User Manual Bruker Optik GmbH...
Page 153
4.8. • only in case of HYPERION 3000: inserting a beam attenuator in the optical adaptation box between microscope and spectrometer (See fig. 7.1.) Transmission mode: Remove the sample from the microscope stage.
Page 154
Sample does not reflect light at all or has a stage in x- and/or y-direction. very poor light reflecting capacity. If you do not succeed in finding a reflective sample area, the sample is not suited sample viewing in the reflection mode. HYPERION User Manual Bruker Optik GmbH...
4.8. • only in case of HYPERION 3000: inserting a beam attenuator in the optical adaptation box between microscope and spectrometer (See fig. 7.1.) Bruker Optik GmbH HYPERION User Manual...
See section 2.7.3. Activate this function in OPUS Joystick control fails. by selecting in the Measure- OPUS, function ment menu the Motorized enabling a joystick-controlled Stage Control function and is deactivated. activating the Activate joystick checkbox. HYPERION User Manual Bruker Optik GmbH...
Connect it cor- ceed in find the focus position nected or not connected prop- rectly. because the In-focus LED erly to the microscope. ☞ See section 4.12.3. does not indicate a focus posi- tion at all. Bruker Optik GmbH HYPERION User Manual...
Page 158
☞ See section 4.12.5. Clean the ATR crystal. • ATR crystal is dirty. ☞ See section 4.12.2. HYPERION User Manual Bruker Optik GmbH...
Imaging device. ☞ For information about how to setup the Note: Autofocus is an electronic option. imaging device, refer to the OPUS/VIDEO Manual. Bruker Optik GmbH HYPERION User Manual...
See fig. 7.1. Check the cable for correct connection. Con- The power supply cable and/or the signal cable nect it correctly. is/are not connected at all or not connected properly. ☞ See section 2.7.6. HYPERION User Manual Bruker Optik GmbH...
Page 161
☞ See section 5.4. Detector oversaturation (even at high wavenumbers; IMAC) Possible cause Solution Insert the beam attenuator in the optical adap- IR signal intensity is too high. tation box. See fig. 7.1. Bruker Optik GmbH HYPERION User Manual...
Page 162
Note: This box is only required for coupling a HYPERION 3000 micro- scope to a FT IR spectrometer. Figure 7.1: HYPERION 3000 with FPA detector (coupled to a FT IR spectrometer by means of an optical adaptation box) HYPERION User Manual...
Pollution degree: 2 according to EN 61010-1 or IEC 60664-1 Protection class: I according to IEC 61140 Objectives Objective Objective Visible Numerical Working magnification magnification aperture distance* (in mm) 4x objective (VIS) 40x objective (VIS) 400x 0.65 15x objective (IR) 150x Bruker Optik GmbH HYPERION User Manual...
100 mm x 80 mm Positioning accuracy ± 0.1 µm at measurement point Repeatability better than1 µm Manual microscope stage Parameter Specification max. travel range in x- and y-direction 50 mm x 75 mm HYPERION User Manual Bruker Optik GmbH...
Specifications A Detectors Detectors for HYPERION 1000 and HYPERION 2000 The detectors listed in the table below are single-element detectors. Mode of cooling / Detector Spectral range Detection sensitivity nominal hold time Standard MCT detector with liquid N 12,000 - 600 cm D*>2x10**10cm Hz...
Specifications A FPA detectors for HYPERION 3000 The FPA detectors listed in the table below are multi-element detectors. Detector Number of Spectral range Mode of cooling / detector elements Nominal hold time photovoltaic MCT 16,384 elements with liquid N 4,500 - 900 cm...
Dimension of the heatable sample holder 75 mm x 50 mm x 10 mm Dimension of the temperature controller 260 mm x 150 mm x 270 mm Cable length ~ 50 cm Power supply requirements 230V, 100W, 50/60Hz Bruker Optik GmbH HYPERION User Manual...
Page 168
Specifications A HYPERION User Manual Bruker Optik GmbH...
(e.g. regarding data acquisition, the optical components to be used etc.) and save them in an experiment file (’*’.xpm). Depending on the combination of HYPERION variant and measurement technique, OPUS includes experiment files with appropriate default parameter settings. These files...
Page 170
Figure A.1: OPUS dialog Video assisted Measurements - Dialog page Optics parameter 1. Mapping measurements are only possible if the microscope is equipped with a motorized sample stage. 2. The FPA detector is only available for the microscope variant HYPERION 3000. HYPERION User Manual...
Page 171
☞ The parameter settings and values on the other dialog pages (Advanced, Acquisi- tion and FT) depend on the FT IR spectrometer to which the HYPERION micro- scope is coupled. For information about the default settings of these parameters, refer to the User Manual of the FT IR spectrometer in question. Consult also the OPUS Reference Manual.
Page 172
Measurement parameters B HYPERION User Manual Bruker Optik GmbH...
Dimensions: 30 mm x 24 mm x 3 mm Pack of 5 mirrors A697-R10 Ge crystal, replacement ATR crystal assembly for ATR objective in case of HYPERION 1000 and HYPERION 2000 A697-R10B Ge crystal, replacement ATR crystal assembly for ATR objective in case of HYPERION 3000...
Page 174
Spare parts and consumables C HYPERION User Manual Bruker Optik GmbH...
System diagram Bruker Optik GmbH HYPERION User Manual...
Page 176
System diagram D HYPERION User Manual Bruker Optik GmbH...
Page 177
Number of detector elements Beam attenuator 76 ......57 ON / OFF switch Beam path ..........49 ......128 Removing / reinstalling .......49 HYPERION 1000 and 2000 ........164 Spectral range ........50 HYPERION 3000 Binocular ....26 Brightness Bruker Optik GmbH HYPERION - User Manual...
Page 179
Viewing and measuring a sample Transmission mode button ....29 Video camera ........26 Video camera port ........52 Viewing mode ..........88 ATR objective .........101 GIR objective ..........29 Microscope VIS button ......29 VIS polarizer ..120 Bruker Optik GmbH HYPERION - User Manual...
Page 180
HYPERION - User Manual Bruker Optik GmbH...
Need help?
Do you have a question about the HYPERION and is the answer not in the manual?
Questions and answers