Turner Designs CyanoFluor User Manual

Handheld hab indicator

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P/N: 998-8701
Revision: A
February 17, 2017
TURNER DESIGNS
st
1995 N. 1
Street
San Jose, CA 95112
Phone: (408) 749-0994
FAX: (408) 749-0998

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Summary of Contents for Turner Designs CyanoFluor

  • Page 1 P/N: 998-8701 Revision: A February 17, 2017 TURNER DESIGNS 1995 N. 1 Street San Jose, CA 95112 Phone: (408) 749-0994 FAX: (408) 749-0998...
  • Page 2: Table Of Contents

    Downloading Data Sample Analysis Guidelines Measurement Parameters Phycocyanin (PC) to Chlorophyll (CHL) Ratio Table Turbidity Effects CDOM Effects External Calibrations to Track Algal Abundance Warranty Terms Warranty Service Out of Warranty Service Appendixes Specifications Warning Messages CyanoFluor User’s Manual Rev. A...
  • Page 3 WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT (WEEE) DIRECTIVE Turner Designs is in the business of designing and selling products that benefit the well-being of our environment. Accordingly, we are concerned with preserving the surroundings wherever our instruments are used and happy to work with customers by complying with the WEEE Directive to reduce the environmental impact resulting from the use of our products.
  • Page 4: Introduction

    HABs and alert users so the proper protocols can be implemented to prevent or reduce harmful bloom effects. CyanoFluor provides raw fluorescence readings that can also be used for determining algal abundances and reporting µg/L chlorophyll estimates.
  • Page 5: Inspection

    CyanoFluor is factory calibrated; no calibration is necessary.  The plastic battery tab at the back of the CyanoFluor must be removed before use. Grasp it firmly and pull gently, removing it to activate the batteries. Grasp the plastic tab firmly and pull gently to remove.
  • Page 6 Although the CyanoFluor floats, do not submerge it in water.  Do not expose the CyanoFluor to temperatures outside the specified range of 5 to 40 °C or damage may occur to the unit that will not be covered under warranty.
  • Page 7: Instrument Operation And Calibration

    (MM/DD/YYYY) and (hh:mm). Use the  keys to select either parameter; a marker will indicate selection. o If MM/DD/YYYY is selected, press ENTER and you will be allowed to adjust the month, date, and year using the  keys. Press ENTER to save the set date. CyanoFluor User’s Manual Rev. A...
  • Page 8: Instrument Power Up

    2.3 for details. 2.2 Instrument Power Up Press the ON/OFF button to power on the CyanoFluor. After a 5 second warm up, the CyanoFluor is ready for operation. Pressing the ON/OFF button again will turn the unit off or if left idle on the HOME screen for 3 minutes the unit will automatically turn off to save battery power.
  • Page 9: Calibration Check

    2.3 Calibration Check CyanoFluor is factory calibrated. Users can check calibration stability using the Cal Check Standard P/N 8700-951. Cal Check Standard The Cal Check Standard P/N 8700-951 is a sealed glass 10x10 mm cuvette containing a specific, non-toxic fluorophore.
  • Page 10: Sample Analysis Procedure

    11) Press the down arrow key again to display FTR Blank values for both PC & CHL channels if FTR Blank was ON and a filtrate blank was measured PC Channel Filtrate Blank RFU PBLK FILT: 25.00 CBLK FILT: 12.00 CHL Channel Filtrate Blank RFU CyanoFluor User’s Manual Rev. A...
  • Page 11: Filtering Samples For Dom Correction

    NOTE: See Sample Analysis Procedure section 2.5 step 9 for result displays. 2.7 Viewing Data Data can be viewed on the CyanoFluor’s display as well as downloaded to a computer. To view data from the HOME screen, use the  arrow keys to scroll to a desired sample, sample number will be displayed in the bottom right corner of the screen.
  • Page 12 8) Click Save and then click the Start button on the software’s screen. 9) Press the ON/OFF button to turn the CyanoFluor on and wait for the 5-second countdown. 10) Press the CyanoFluor keypad’s DATA button; use the  arrow keys to select SEND/CLEAR DATA.
  • Page 13: Sample Analysis Guidelines

     Use the same cuvette for your samples, it is very important that you thoroughly rinse the cuvette between samples. CyanoFluor is very sensitive, therefore cross contamination between subsequent samples will skew results. Three rinses with the sample intended for measurement will help flush out any residual and decrease the chance for cross contamination.
  • Page 14: Measurement Parameters

    Quick Start Guide or Section 2.5/2.6 of this manual. This will correct for any interference or error in estimate due to a high background fluorescence which may be cause by dissolved organic materials. CyanoFluor User’s Manual Rev. A...
  • Page 15: External Calibrations To Track Algal Abundance

    CyanoFluor using natural water samples: 1) Take a grab sample from your aquatic habitat 2) Measure the sample using the CyanoFluor and record the RFU value for chlorophyll 3) Use extraction methods (EPA method 445.0) to determine the actual chlorophyll µg/L concentration of the water sample measured 4) Create a conversion factor that converts RFU to µg/L...
  • Page 16: Terms

    (RMA). Package the unit, write the RMA number on the outside of the shipping carton, and ship the instrument, prepaid, to Turner Designs. If the failure is covered under the warranty terms, the instrument will be repaired and returned free of charge, for all customers in the contiguous continental United States.
  • Page 17 CyanoFluor User’s Manual Rev. A...
  • Page 18: Appendixes A Specifications

    Case Meets IP 67 Standard; dustproof and waterproof Temperature 41-104 °F; 5-40 °C Internal Memory 1,000 records Data Output Format ASCII Weight 0.87 lbs (0.4kg) 1.75” x 3.5” x 7.25”(4.45cm x 8.9cm x 18.4cm) Size CyanoFluor User’s Manual Rev. A...
  • Page 19: B Warning Messages

    Appendix B: Warning Messages Memory Warning Messages CyanoFluor’s memory starts at 100% allowing for a maximum of 1,000 data points to be logged internally. If the memory is more than 90% full and the READ button is pressed, the following message will display: “Warning: Memory >...

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