GeneAmp PCR System 9700 User Manual
  1. Manuals
  2. Brands
  3. GeneAmp Manuals
  4. Control Unit
  5. PCR System 9700
  6. User manual

GeneAmp PCR System 9700 User Manual

0.5ml sample block module
Hide thumbs
Table of Contents

Advertisement

Quick Links

GeneAmp
PCR System 9700
®
0.5mL Sample Block Module
User's Manual

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the PCR System 9700 and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Summary of Contents for GeneAmp PCR System 9700

  • Page 1 GeneAmp PCR System 9700 ® 0.5mL Sample Block Module User’s Manual...
  • Page 2 NOTICE TO PURCHASER:LIMITED LICENSE The combination of the GeneAmp ® PCR System 9700 Sample Block Module with a GeneAmp ® PCR System 9700 Base Unit is licensed under patent claims for thermal cycler apparatus and counterpart patent claims in other countries. No other rights are conveyed expressly, by implication, or by estoppel.

  • Page 3: Table Of Contents

    Contents 1 0.5-mL Sample Block Module Safety and Regulatory Information ..........1-1 General Symbols .
  • Page 4 Loading Samples into the Reaction Tubes ........1-17 Placing the Reaction Tubes into the Sample Block .

  • Page 5: Safety And Regulatory Information

    0.5-mL Sample Block Module Safety and Regulatory Information IMPORTANT Read this section before you install the GeneAmp ® PCR System 9700 and the 0.5-mL Sample Block Module. General Symbols The following symbols are used on Applied Biosystems instruments. Whenever such symbols appear on instruments, please observe appropriate safety procedures.

  • Page 6: Instrument Labels

    This symbol indicates the presence of high voltage and warns the user to proceed with caution. This symbol alerts you to consult the manual for further information and to proceed with caution. Non-electrical Symbols This symbol illustrates a heater hazard. Proceed with caution when working around these areas to avoid being burned by hot components.

  • Page 7: Operating Precautions

    Operating Precautions Precautions The following precautions should be taken whenever you operate the system 9700 with the 0.5-mL Sample Block Module installed. Note This instrument is able to withstand transient overvoltage according to Installation Category II as defined in IEC 1010-1. General Use CAUTION The instrument should be used according to the...

  • Page 8: Grounding And Electrical Safety

    Do not use an adapter to a two-terminal outlet since this does not provide positive ground protection. ® Refer to the GeneAmp PCR System 9700 Base Module User’s Manual (P/N 0993-6247) for more information on electrical safety. Voltage Quality Line voltage must be within 10% of the nominal value.

  • Page 9: Pollution Category

    Pollution Category The system 9700 will operate safely in environments that contain nonconductive foreign matter up to Pollution Degree 2 in IEC 1010-1. Instrument Storage The system 9700 must be stored at a temperature between -20 °C and 60 °C (-4 °F and 140 °F) at altitudes ranging from 0–12,000 meters above sea level.

  • Page 10: Technical Support

    Technical Support To Reach Us on the Applied Biosystems web site address is: http://www.appliedbiosystems.com/techsupport We strongly encourage you to visit our web site for answers to frequently asked questions, and to learn more about our products. You can also order technical documents and/or an index of available documents and have them faxed or e-mailed to you through our site (see the “Documents on Demand”...

  • Page 11: Documents-On-Demand

    For Support On This Product Dial 1-800-831-6844, and... PCR and Sequence Detection Press 5, or call 240-453-4613 1-800-762-4001, and press 1 for PCR, or 2 for Sequence Detection FMAT Telephone 1-800-899-5858, 508-383-7855 and press 1, then press 6 Peptide and Organic Synthesis Press 650-638-5981 Protein Sequencing...

  • Page 12: To Reach Us By E-Mail

    If you want to order... Then... by phone from a. Dial your international access code, then 1-858-712-0317 from a outside the United touch-tone phone. States and Have your complete fax number and country code ready (011 Canada precedes the country code). b.
  • Page 13 Europe Finland (Espoo) Poland, Lithuania, Latvia, and Estonia (Warszawa) Tel: 358 (0)9 251 24 250 Fax: 358 (0)9 251 24 243 Tel: 48 (22) 866 40 10 Fax: 48 (22) 866 40 20 France (Paris) Portugal (Lisboa) Tel: 33 (0)1 69 59 85 85 Tel: 351 (0)22 605 33 14 Fax:...
  • Page 14 Eastern Asia, China, Oceania Hong Kong Taiwan (Taipei Hsien) Tel: 852 2756 6928 Tel: 886 2 2698 3505 Fax: 852 2756 6968 Fax: 886 2 2698 3405 Korea (Seoul) Thailand (Bangkok) Tel: 82 2 593 6470/6471 Tel: 66 2 719 6405 Fax: 82 2 593 6472 Fax:...

  • Page 15: About The 0.5-Ml Sample Block Module

    (PCR) process. The 0.5-mL Sample Block Module attaches to the top of the GeneAmp ® PCR System 9700 Base Module and allows you to perform up to 60 reactions. You can remove the 0.5-mL Sample Block Module and replace it with another sample block module as needed.

  • Page 16: Sample Block

    Sample Block The sample block has 60 wells (10 x 6) for use with GeneAmp ® Thin-Walled Tubes with Flat Caps (0.5-mL volume). The sample block can operate in one of two modes: Sample control (0.5-mL Mode button) In this mode, the instrument calculates the temperature of the sample during cycling.

  • Page 17: Heated Cover

    Heated Cover The heated cover slides over the sample block and performs two functions: It raises the temperature of the upper part of the tube (the part above the sample block) above the temperature of the sample mixture. This eliminates condensation, which may have a negative effect on your chemistry.

  • Page 18: Modules, Accessories, And Disposables

    Modules, Accessories, and Disposables Overview ® The modules and accessories listed below are available for the GeneAmp System 9700 Base Module. The disposables are for use with the 0.5-mL Sample Block Module. Ordering Order modules, accessories, and disposables from Applied Biosystems by part number.

  • Page 19: Installing The 0.5-Ml Sample Block Module

    Installing the 0.5-mL Sample Block Module Overview ® A lever behind the sample block module releases it from the GeneAmp PCR System 9700 Base Module. Diagrams of the top and bottom of the 0.5-mL Sample Block Module are shown below.
  • Page 20 Installing the To install the sample block module into the GeneAmp ® PCR System 9700 Base Module: Sample Block Module Step Action Pull the lever out from the sample block module. Place the sample block module onto the base module, then push the sample block module back to seat the electrical connections.

  • Page 21: Overview

    ® GeneAmp Thin-Walled Tubes 0.5-mL Thin-Walled Tubes with Flat Caps Thermal with Flat Caps Insulation Frame GeneAmp 0.5-mL Thermal Insulation Frame Sample block Loading Samples To load samples into the reaction tubes: into the Reaction Step Action Tubes Pipette samples into the reaction tubes.
  • Page 22 Sample Block Place the GeneAmp Thin-Walled Tubes with Flat Caps directly into the sample block. Set the GeneAmp 0.5-mL Thermal Insulation Frame over the tubes. See the diagram below. IMPORTANT Be sure to use the frame. It insulates your samples and prevents the heated cover from damaging the reaction tubes.

  • Page 23: Cleaning The Sample Block Module

    Cleaning the Sample Block Module Cleaning: Overview The following procedures describe: The cleaning position of the sample block module How to clean the heated cover How to clean the sample block wells IMPORTANT Before using any cleaning or decontamination method, except those recommended in this manual, check with Applied Biosystems to ensure that the proposed method will not damage the equipment.

  • Page 24: Cleaning The Sample Block Wells

    To clean the heated cover: (continued) Step Action If the cover becomes contaminated with amplified DNA: Raise the heated cover to the cleaning position. Wipe the cover with a cloth or cotton swab soaked in 10% bleach. Wipe with a damp cloth. Cleaning the Sample Clean the sample block wells once a month or more frequently if needed.

  • Page 25: Running The Calibration Verification Test

    Running the Calibration Verification Test Overview ® Use this test to verify the temperature calibration of your GeneAmp PCR System 9700 0.5-mL Sample Block Module. To complete the Calibration Verification Test, you will perform the following procedures: Step Action See Page Setting Up the 0.5-mL Probe Assembly...
  • Page 26 If the heated cover is in the forward position, lift the lever, then slide the heated cover back. Place the GeneAmp 0.5-mL Thermal Insulation Frame on the sample block. Use a cotton swab to coat well A6 with mineral oil.
  • Page 27 Configuring the To configure the system 9700 for the Calibration Verification Test: System 9700 Complete the procedures in “Setting Up the 0.5-mL Probe Assembly” on page 1-22. Turn on the system 9700. The main menu appears. Press Util. The Utilities screen appears. Note To press the menu items, use the corresponding F keys.
  • Page 28 Running the Test Use the digital thermometer to take temperature readings of the sample well connected to the 0.5-mL Probe Assembly. You will take a reading at two different setpoint temperatures. Note If necessary, press Cancel to exit the test. To run the Calibration Verification Test: Step Action...
  • Page 29 To run the Calibration Verification Test: (continued) Step Action Using the numeric keys, type the value displayed on the digital thermometer in the “Enter actual block temperature” field. Calibration Verification Block temp = xx.x°C Cover temp = xxx°C Enter actual block temperature xx.x Cancel Note The digital thermometer displays a four-digit value;...
  • Page 30 To run the Calibration Verification Test: (continued) Step Action When you have completed all measurements, be sure to: Press Exit. Remove the 0.5-mL Probe Assembly from the sample block. Turn off the digital thermometer and clean off the oil. Remove the 0.5-mL Thermal Insulation Frame from the sample block. IMPORTANT Make sure the sample block is at room temperature (~25 °C) before removing the frame.

  • Page 31: Running The Temperature Non-Uniformity Test

    Running the Temperature Non-Uniformity Test Overview ® Use this test to verify the temperature uniformity of the GeneAmp PCR System 9700 0.5-mL Sample Block Module. To complete the Temperature Non-Uniformity Test, you will perform the following procedures: Step Action See Page Setting Up the 0.5-mL Probe Assembly...
  • Page 32 If the heated cover is in the forward position, lift the lever, then slide the heated cover back. Place the GeneAmp 0.5-mL Thermal Insulation Frame on the sample block. Use a cotton swab to coat the following wells with mineral oil: Place the 0.5-mL Probe Assembly into well A1.
  • Page 33 Configuring the To configure the system 9700 for the Temperature Non-Uniformity Test: System 9700 Complete the procedures in “Setting Up the 0.5-mL Probe Assembly” on page 1-28. Turn on the system 9700. The main menu appears. Press Util. The Utilities screen appears. Note To press the menu items, use the corresponding F keys.
  • Page 34 Running the Test The Temperature Non-Uniformity Test uses the 0.5-mL Probe Assembly to test the temperature uniformity of 12 different wells in the sample block. Note If necessary, press Cancel to exit the test. To run the Temperature Non-Uniformity Test: Step Action Complete the procedures in “Setting Up the 0.5-mL Probe Assembly”...
  • Page 35 To run the Temperature Non-Uniformity Test: (continued) Step Action Using the numeric keys, type the value displayed on the digital thermometer in the “Enter actual block temperature” field. TNU Performance Sample temp = xx.x°C Cover temp = xxx°C Enter actual block temperature 00.0 Cancel Note The digital thermometer displays a four-digit value;...
  • Page 36 To run the Temperature Non-Uniformity Test: (continued) Step Action Slide the heated cover back and repeat steps 4–7 of “Setting Up the 0.5-mL Probe Assembly” on page 1-28 and steps 2–7 of this procedure. Complete these steps for all 12 wells to be tested: The system 9700 evaluates the uniformity of the sample block temperature for the setpoint values you entered and displays the results.

  • Page 37: Evaluating The Results

    Evaluating the When the system 9700 completes the Temperature Non-Uniformity Test, the TNU Performance screen appears. See the table below to evaluate the results. Results If the... Then... Temperature of the “Pass” appears after each setpoint temperature. sample block wells is uniform, TNU Performance TNU at 94°C is xx.xx - Pass...

  • Page 38: Running System Performance Diagnostics

    Cancel Cont Note You will install the GeneAmp 0.5-mL Thermal Insulation Frame. Place the 0.5-mL Thermal Insulation Frame in the sample block. Slide the heated cover forward and pull the lever down. After you have installed the frame, press Cont.
  • Page 39 To run the Rate Test: (continued) Step Action At the conclusion of the test, the Cool and Heat Rate Test screen appears. The screen displays the test results and whether the test passed or failed. Cool and Heat Rate Test Pass Heating rate: x.xx °C/s Cooling rate: x.xx °C/s...

  • Page 40: Running The Cycle Test

    Cancel Cont Note You will install the GeneAmp 0.5-mL Thermal Insulation Frame. Place the 0.5-mL Thermal Insulation Frame in the sample block. Slide the heated cover forward and pull the lever down. After you have installed the frame, press Cont.
  • Page 41 To run the Cycle Test: (continued) Step Action At the conclusion of the test, the Cycle Performance screen appears. The screen displays the test results and whether the test passed or failed. Cycle Performance Pass Average Cycle Time: xxx.x sec x.x sec Cycle Time STD: Print...

  • Page 42: Data Sheet: Calibration Verification Test

    Data Sheet: Calibration Verification Test Instructions When running the Calibration Verification Test, record the setpoint values for well A6 on this data sheet. At the end of the Calibration Verification Test, check the values displayed on the system 9700 against the values recorded here. This will help maintain accurate test records.

  • Page 43: Data Sheet: Temperature Non-Uniformity Test

    Data Sheet: Temperature Non-Uniformity Test Instructions When running the Temperature Non-Uniformity Test, record the setpoint values for the wells listed on this data sheet. At the end of the Temperature Non-Uniformity Test, check the values displayed on the system 9700 against the values recorded here. This will help maintain accurate test records.
  • Page 44 1-40 0.5-mL Sample Block Module...
  • Page 45 1-4 DNA contamination input/output connections 1-4 heated cover 1-19 instrument storage 1-5 GeneAmp® 0.5-mL Thermal Insulation sample block wells 1-19 physical specifications 1-4 Frame heated cover 1-18 pollution categoy 1-5 part number 1-13 position 1-18 routine maintenance 1-5 GeneAmp®...
  • Page 46 Communautes Europeennes (CE) Compliance 1-5 parts www address electrical 1-1 modules, accessories, and Applied Biosystems 1-6 non-electrical 1-2 disposables 1-13 FAX-on-Demand 1-7 System Performance Diagnostics ordering 1-13 required equipment 1-33 PCR cycling function, system performance tests, testing 1-35–1-36 running 1-33–1-36 Peltier unit Cycle Test, running 1-35–1-36 heating/cooling unit 1-12...
  • Page 48 Worldwide Sales and Support Applied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, reaches 150 countries on six continents. For sales office locations and technical support, please call our local office or refer to our Web site at www.appliedbiosystems.com.

Table of Contents