1. Introduction Product Intended Usage The HEKA LIH 8+8 is intended for research use only in a laboratory by persons trained in its use. Users are expected to be able to properly operate the LIH 8+8 and associated instruments. The HEKA LIH 8+8 is not intended for medical use. The HEKA LIH 8+8 is not intended for use in life support situations, or in situations were improper operation or failure of the LIH 8+8 can result in personal injury.
If any of these items are missing please contact HEKA or your dealer im- mediately. Unpacking After unpacking the LIH 8+8 and accessories from the shipping carton, please inspect each piece for any signs of shipping damage. Please contact the delivering carrier and HEKA immediately if there is any damage. Do not dispose of the shipping carton.
Please note that many newer computer systems are supplied with multi- ple USB 2.0 ports, but only some of these ports are capable of Hi-Speed operation. The LIH 8+8 will work on the slower speed ports, but the maximum transfer rates will be greatly reduced. For optimal performance, the LIH 8+8 should only be connected to a USB 2.0 Hi-Speed port.
All of the digi- tal input and output lines are available on ribbon-cable connectors located on the rear panel. The LIH 8+8 is connected to a host computer’s USB 2.0 Hi-Speed (480 Mbits/second) port over a shielded USB 2.0 cable (3 meters).
1.5.3 Isolation All of the analog input and output channels of the LIH 8+8 are isolated from the digital electronics used to communicate with the computer. This isolation is achieved with state of the art high-speed digital isolator’s. This isolation is provided to eliminate computer electrical noise from the ana- log measurements.
Introduction Both A/D converters sample simultaneously at the maximum conversion rate resulting in a total throughput of 400 kHz. If the bandwidth of the experiment calls for lower sampling rates, then the hardware will adjust the rate accordingly. The simultaneous sampling arrangement allows pairs of channels, for exam- ple the Current and Voltage signals from a Patch Clamp Amplifier, to be digitized without phase-shift.
USB 2.0 Benefits 1.5.7.1 True Plug-and-Play Simply connect the LIH 8+8 to an available Hi-Speed USB 2.0 port on the host computer system using the supplied USB cable. The computer will automatically identify the LIH 8+8 and install the appropriate system drivers, if needed.
Today, virtually all desktop and laptop computers on the market provide USB ports. It is now possible to use low-cost, small form factor systems (i.e. iMac, Mac mini, etc) with the LIH 8+8 for data acquisition applications.
1.5.9 Tone Generator The LIH 8+8 has a built-in tone generator that is controlled by software. The tone generator has a frequency range between 200 Hz and 4 kHz. A 3.5 mm audio connector is provided on the rear panel for connection to an amplified speaker or headset.
2. Technical Specifications Analog Inputs Number of channels: 2 ADC multiplexed into 4 inputs Bank 0 - channels 0,2,4 and 6 Bank 1 - channels 1,3,5, and 7 Input type: differential, isolated Input connector: BNC on front panel Resolution: 16-bit, (1 in 65536) Sampling rate: 400 kHz aggregate, 200 kHz per ADC Input range:...
Technical Specifications Trigger Input Number of channels: 1, isolated Input type: 3.3 and 5 Volt logic compatible Minimum pulse width: 30 ns Operational mode: software selectable positive or negative edge transition Input connector: BNC on front panel Maximum over voltage: 30 Volts D.C.
2.6 Tone Generator Tone Generator Frequency range: between 200 Hz and 4 kHz Output connector: 3.5 mm audio jack on rear panel Additional Connectors USB: USB 2.0 female B connector on rear panel Digital IO: 40 pin male connector on rear panel for interfacing with EPC 8 or TIB 14 Master sync: RJ-45 (CAT5) connector on rear panel...
Technical Specifications 2.11 Host Interface USB 2.0: Hi-Speed (480 Mbits/second) 2.12 Warranty Duration: One year parts and labor http://www.heka.com...
If installing on a rack, please do not use the LIH 8+8 as a shelf to support any other instrument. The LIH 8+8 case was not designed to do this and damage to the front panel will result.
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Installation 5. If multiple LIH 8+8 interfaces are to be connected, repeat the steps as outlined above for each unit. In addition, for the acquisition clocks to be properly synchronized, a connection between the Master clock output of one unit to the Slave clock input of the other (connectors located on the rear panel) must be made using standard CAT5 patch cables.
4. Controls and Functions Front-panel Controls Figure 4.1: LIH 8+8 Front Panel Controls 1. DAC Outputs: Four BNC connectors provide access to the four individual 16-bit Digital to Analog converter outputs. The acquisi- tion software controls the D/A channels output voltage and timing.
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LIH 8+8 has been properly initialized and ready for use. 4. External Trigger Input: A BNC connector provides access to the Trigger input circuitry of the LIH 8+8. A TTL trigger pulse can be given to start data acquisition. The LIH 8+8 can be triggered with either a positive or negative logic pulse, depending on software settings, with a minimum duration of 30 ns.
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7. Chassis Ground: A 4 mm banana receptacle provides connection to the chassis ground of the LIH 8+8 interface. 8. Power Indicator LED: The power LED will be illuminated when the LIH 8+8 is connected to the power line and switched ON. http://www.heka.com...
AC power outlet. 3. Fuse: A F1.0A 20 mm fuse is used to protect the internal power supply of the LIH 8+8. If the fuse needs to be replaced, only replace with a 20 mm type F1.0A fuse.
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4.2 Rear panel controls 5. Digital I/O: A 40-pin male connector combines the Digital Output and Input lines of the LIH 8+8. This specialized connector is re- quired for interfacing with other HEKA devices such as the EPC 8 Patch Clamp Amplifier or the TIB 14S Trigger Interface. To mate to this connector use 3M electronics 3417 series or equivalent.
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Controls and Functions 9. Master Sync: A RJ-45 connector provides a 33 MHz clock output for synchronizing the acquisition clocks between LIH 8+8 interfaces. A standard CAT5 patch cable is used to connect the Master output of one unit to the Slave input of the other.
Disconnect ALL external instruments and test the LIH 8+8 /com- puter combination by itself. If possible, swap in a known working LIH 8+8 as a quick way to determine if the problem is the LIH 8+8 or the computer.
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(c) Check that a shielded USB 2.0 cable is used. Non-shielded cables can lead to USB communication problems that manifest in a variety of odd behaviors. (d) To verify that the LIH 8+8 is connected please do the following: i. Windows: A. Click on My Computer.
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CPU speed, hard disk speed, available RAM, etc. The following can be easily verified: (a) Check that the LIH 8+8 is connected to a USB 2.0 Hi-Speed port (as outlined above), and that the port is not shared by any other device.
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Troubleshooting As with all GUI based software application the HEKA software prod- ucts rely on the mouse (or other pointing device) for most of the user interaction. An assumption is made that the user has a working knowledge of the operating systems various input devices and con- ventions.
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(b) Select Set Defaults. The hardware selection window will be displayed. (c) Select the option EPC 7 and others and the interface LIH 8+8. Since these test procedures are geared for testing the LIH 8+8 the amplifier selection of EPC 7 and others is used to basically specify “no amplifier.”...
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LIH 8+8. They are not to be used for testing the functionality of an LIH 8+8 built into an EPC 10 USB Patch Clamp Amplifier. Those test procedures are outside the scope of this manual.
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The sound driver is not properly installed. Select retry to ini- tialize the driver once more or abort to terminate the sound driver initialization. If abort, is selected then LIH 8+8 initial- ization will continue, since this is not considered a fatal error.
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D/A channels is with either a Digital Multimeter (DMM or DVM) or an oscilloscope. One of the LIH 8+8 A/D inputs can also be used (if it is known to be working and properly calibrated).
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(h) Checking the A/D Inputs: The most accurate way to verify the operation of the A/D channels is with a calibrated voltage source. If the D/A channels of the LIH 8+8 have been verified they can be used. i. Assuming that a D/A output to be used has been verified (refer to instructions above for reference) connect the D/A to the A/D channel to be tested.
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Troubleshooting a breakout cable will have to be fabricated. Please refer to Appendix I of this manual for a complete pin-out description. Important note: It may be tempting to insert the tip of either the oscilloscope probe or the DMM directly into the DB-25 connector, but it is not rec- ommended.
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(j) Checking the Digital Inputs: A device that can output TTL HIGH (5 Volts) is required to test the functionality of the Digital Inputs. The LIH 8+8 itself can provide this output either with one of the D/A or Digital Output channels.
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To resolve these problems, please try the following: (a) Verify that the LIH 8+8 and the host computer are plugged into a common outlet strip. This will minimize power line induced noise (sixty / fifty hertz signals) in the acquired data.
6. Warranty and Support Warranty HEKA warrants to the first purchaser, for a period of one year from the date of purchase (invoice date), that this HEKA Instrument (hereafter referred to as the Product) will be free from defects in workmanship and materials, and agrees that it will, at its option, either repair the defect or replace the defective Product or part thereof at no charge to the purchaser for parts and labor.
Warranty and Support ware updates to increase the capabilities of the present Product are not to be provided under the terms of this warranty. Updates will be available for free download on www.heka.com. No sales organizations, other than HEKA itself, are authorized to make any warranties other than those described above, or to extend the duration of any warranties beyond the time period described above on the behalf of HEKA.
6.3 Obtaining Warranty Service breach of contract, warranty, or otherwise, shall HEKA, or its subcontrac- tors or suppliers, be liable for any special consequential, incidental, indirect or exemplary damages, including, but not limited to, loss of profit or rev- enues, loss of use of the equipment or any associated equipment, cost of capital, cost of substitute goods, facilities, services or replacement power, downtime costs or claims of Customers clients for such damages.
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Warranty and Support able that may help resolve product-specific questions. If the problem is not resolved, please supply the following information when corresponding: Serial number of the LIH 8+8 interface Operating system version Driver software version number ...
7. Appendix I: Technical Data DIGITAL I/O Connector This is a 40-pin male connector that combines the digital input and output lines. This connector is to be used to interface to other HEKA devices, such as the EPC 8 or the TIB 14. For other purposes, the DIGITAL IN and DIGITAL OUT Connectors should be used.
Appendix I: Technical Data Figure 7.1: Digital I/O Connector Pin number Signal Pin number Signal IN-0 OUT-14 NO CONNECTION OUT-15 IN-2 IN-10 IN-3 IN-11 IN-4 IN-12 IN-5 IN-13 IN-6 IN-14 IN-7 IN-9 IN-1 NO CONNECTION NO CONNECTION STROBE OUT-0 OUT-8 OUT-1 OUT-9 OUT-2...
7.2 DIGITAL IN Connector DIGITAL IN Connector This is a DB-25 female connector that can be used to interface with the Digital Input lines of the LIH 8+8. Figure 7.2: Digital IN Connector Pin number Signal Pin number Signal IN-3...
Appendix I: Technical Data DIGITAL OUT Connector This is a DB-25 female connector that can be used to interface with the Digital Output lines of the LIH 8+8. Figure 7.3: Digital OUT Connector Pin number Signal Pin number Signal OUT-3...
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