Growth Curves Bioscreen C User Manual
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Growth Curves Bioscreen C User Manual

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User's Manual
Bioscreen C
Oy Growth Curves Ab Ltd
2017
Version 2.1.12

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Summary of Contents for Growth Curves Bioscreen C

  • Page 1 User's Manual Bioscreen C Oy Growth Curves Ab Ltd 2017 Version 2.1.12...

  • Page 3: Table Of Contents

    Table of Contents Inspection and Installation of the Bioscreen C System System Description Overview Bioscreen C Reader Software Operation Pre-Start Checklist Planning an Experiment Running an Experiment Data Handling Guide to Anaerobic Experiments Guide to Manual Pipetting Maintenance Maintenance Steps & Frequency...
  • Page 4 We hope this system fulfills your expectations. If you should have any questions or problems, please don’t hesitate to contact your local Bioscreen C distributor. Have a nice time with your versatile Bioscreen C! Lamp and Incubator compartment: filter wheel...

  • Page 5: Inspection And Installation Of The Bioscreen C System

    THE BIOSCREEN C SYSTEM It is very important to read these instructions carefully and to follow them in the presented order (from 1 to 11). Wrong order might cause problems with the installation or the use of the Bioscreen C system.
  • Page 6 11. Run a short test measurement Run a short test measurement with the BioScreener software to verify that your Bioscreen C system is working properly. More details on how to run an experiment with BioScreener software can be found from software’s internal help section.

  • Page 7: System Description

    For vertical light photometry, all visible light wavelengths can be used. The eight standard filters provided in the filter wheel of Bioscreen C are: 405, 420, 450, 492, 540, 580, 600 nm and a wide band (420-580 nm) filter. Filters with special wavelengths are available by request.

  • Page 8: Software

    ® The BioScreener software is designed to run Bioscreen C experiments. BioScreener SW is a combination of the proven functions of the previous softwares used with Bioscreen C. BioScreener stores all settings and results from the experiment in a .bsm file. Results can be exported to common data files such as xls or csv for graph generation and analysis with another software of your choice.

  • Page 9: Operation

    60 C is reached when the incubator cover is 60 C and the incubator tray holding the samples has reached 59 C. Bioscreen C can reach 6 C below and 30 C above ambient temperature (see Figure 4). If lower temperatures are desired, the whole Bioscreen C must be placed in a cooled room.
  • Page 10 3.2.5 Shaking BioScreener software has various shaking protocols. It also includes parameters from previous Bioscreen C softwares (eg. EZExperiment SW and BioLink SW), which can be used by selecting appropriate compatibility mode from BioScreener’s Additional options menu.

  • Page 11: Running An Experiment

    3.3 Running an Experiment Switch ON the Bioscreen C and your PC. Open the the cover of the incubator compartment (see. Figure 1). Remove the black incubator cover by first pulling gently the spring catch (with the text “OPEN”) towards you.
  • Page 12 Bioscreen C Microbiology Reader User's Manual _______________________________________________________________________________________________ ________ Figure 5 Inserting the incubator cover properly to the hinges. Figure 6 Checking the seam of the parts. _______________________________________________________________________________________________ ________...

  • Page 13: Data Handling

    This approach lets the user handle Bioscreen C data (curve drawing etc.) using any DOS, OS/2 or Windows graphical, scientific or statistical software.

  • Page 14: Guide To Manual Pipetting

    An indicator liquid or paper is placed into one well for confirming the existence of anaerobiosis. If O leaks, the colour of the indicator liquid or paper changes. The hermetically closed Honeycomb microplate is put into the Bioscreen C and a normal experiment with shaking, incubating and measurement is carried out.

  • Page 15: Maintenance

    Bioscreen C Microbiology Reader User's Manual _______________________________________________________________________________________________ ________ 6. MAINTENANCE 6.1 Maintenance Steps & Frequency During normal operation we recommend you to perform the following maintenance procedures:  Clean the lenses Weekly / more often if necessary  Clean the filter wheel Weekly ...

  • Page 16: Cleaning

    Remember to clean the contaminated parts properly after use to avoid health risks. This is particularly important when Bioscreen C is sent out of the laboratory or if a service visit is needed. Your service representative will ask you to provide a written certificate of decontamination.

  • Page 17: Checking The Heat Transfer Fluid Level

    Check the level of the heat transfer fluid at the window of the incubator at the front of the instrument regularly. With the Bioscreen C turned ON, the green lamp in the tank window lets you see the level of the heat transfer fluid.

  • Page 18: Replacing The Lamp

     Make sure that your new lamp is sold and pre-tested by Growth Curves Ltd. (see Figure 10).  Install the new lamp so that the red dot points upwards. Do not bend the lamp pins. Do not touch the reflective surface of the bulb.
  • Page 19 Lamps tested, approved and marked by Growth Curves Ltd. There are some brands of lamps that will not give enough light for the wavelength range that the Bioscreen C operates. Only lamps that are sold and pre-tested by Growth Curves Ltd. are guaranteed to operate properly.

  • Page 20: Troubleshooting Guide

    Bioscreen C Microbiology Reader User's Manual _______________________________________________________________________________________________ ________ 7. TROUBLESHOOTING GUIDE 7.1 Optical problems Problem Filter error (FE) A filter is missing. Cause Be sure that the filter wheel is properly installed. Solution Problem Blank error (F1 - B2) Insufficient light to the amplifier:...
  • Page 21 Bioscreen C Microbiology Reader User's Manual _______________________________________________________________________________________________ ________ 1) Use shaking before each measurement to keep the sample suspension Solution homogenous. Try different shaking options for the best result. 2) Do not open the cover during the measurement. 3) Place the instrument into a room or cabinet of higher or lower temperature.

  • Page 22: Temperature Problems

    3) Press RESET on Bioscreen C keypad. 4) Check the COM Port settings from the BioScreener’s Additional Options menu. 5) Use the cable supplied with Bioscreen C. Bioscreen C is delivered with a standard serial cable and USB-adapter. Note _______________________________________________________________________________________________...
  • Page 23 Bioscreen C Microbiology Reader User's Manual _______________________________________________________________________________________________ ________ Problem Totally wrong readings periodically during a long experiment. Computer power saving features interfere with serial port communication. Cause Turn off all power saving features from your computer. Solution Please consult with your IT department if necessary.

  • Page 24: About Bioscreen

    Other readers are not designed for microbiology work, or for long-term use with an incubating time of up to 1600 hours. Bioscreen C has proven its versatility for a wide range of applications in over 500 sites worldwide, with some units in use for over 20 years.
  • Page 25 Even anaerobic bacteria can be grown in Bioscreen C. This can be done by sealing the cover of the microplate and using an oxygen-free gas, alternatively the oil layer technique can be used (see chapter “4.

  • Page 26: Technical Specifications

    Bioscreen C Microbiology Reader User's Manual _______________________________________________________________________________________________ ________  To develop bio energy applications.  LAL tests (Limulus amoebocyte lysate gel assay) for endotoxin assay.  Mathematic modeling of microorganism, phage or cell growth with the help of MS Excel or similar softwares.

  • Page 27: Photometric System

    Bioscreen C Microbiology Reader User's Manual _______________________________________________________________________________________________ ________ NOTE 1 Installation category (over-voltage category) defines the level of transient over-voltage, which the instrument is designed to withstand safely. It depends on the nature of the electricity supply and its over-voltage protection means. For example in CAT II which is the category used for...

  • Page 28: Pc Requirements

    Bioscreen C Microbiology Reader User's Manual _______________________________________________________________________________________________ ________ Capacity: 100 wells per one Honeycomb microplate. Use 1 or 2 microplates per experiment. 400 l / Honeycomb microplate well Maximum volume: Material: Polystyrene Sterilizing method: Gamma radiation Note: Honeycomb 2 microplates are single use consumables.

  • Page 29: User Replaceable Parts

    240 5590 Filter wheel with 405, 420, 450, 492, 540, 580, 600 and WB (420-580) nm filters 240 0620 Halogen lamp 8V 50W – use ONLY lamps that are pre-tested by Growth Curves Ltd 121 0051 Fuse 2A slow, 10 pcs...
  • Page 30 Bioscreen C Microbiology Reader User's Manual _______________________________________________________________________________________________ ________ _______________________________________________________________________________________________ ________...

  • Page 31: Appendix

    Growth Curves Ltd. will repair or replace defective parts or materials during the term of warranty at no extra charge for materials and labor, provided that the products were used and maintained according to Growth Curves Ltd’s instructions.

  • Page 32: Appendix

    12. Appendix Bioscreen C Service Request Serial Number: ___________________________________________ Owner (Company): ___________________________________________ Contact Person: ___________________________________________ Contact Phone: ___________________________________________ Contact Fax: ___________________________________________ Contact E-mail: ___________________________________________ Date: ___________________________________________ Include this document with the instrument packaging.  Maintenance/Service 1. Instrument is sent for ...
  • Page 33 Safety/shipping label Fill out and sign the Safety/Shipping Label below. Cut out and affix it to the exterior (outside) of the shipping carton. Safety/Shipping Label TO: Growth Curves Ltd Telekatu 12 20360 Turku FINLAND Samples used in product:...
  • Page 34 Responsibilities It is the responsibility of the Bioscreen user to 1. Remove all substances which are dangerous for human health from the instrument so that the instrument is safe for human to handle. 2. Choose the appropriate decontamination method depending on what substances (liquids) the instrument has been exposed to.

  • Page 35: Bioscreen C Certificate Of Decontamination

    Bioscreen C Certificate of Decontamination Serial Number: ___________________________________________ Owner (Company): ___________________________________________ Contact Person: ___________________________________________ Contact Phone: ___________________________________________ Contact Fax: ___________________________________________ Contact E-mail: ___________________________________________ Date: ___________________________________________ Include this document with the instrument packaging. Decontamination Information: Materials used in unit:  Carcinogen ...
  • Page 36 This page is reserved for your notes:...
  • Page 38 This User’s Manual covers the use, specifications and maintenance of Bioscreen C Microbiology Reader. Information in this User´s Manual is subjected to change without prior notice. Web databases on Bioscreen C: www.bioscreen.fi www.growthcurvesusa.com Oy Growth Curves Ab Ltd www.bioscreen.fi Vuorimiehenkatu 13 A 2, 00140 Helsinki, Finland...

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