Table of Contents

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List .................................................................................................................................................... 1

Chapter 1 Instrument Specification ................................................................................................... 2
Chapter 2 Installation ........................................................................................................................ 9
Chapter 3 Performance And Test Flow .............................................................................................. 12
Chapter 4 Module Instruction ................................................................................21
Chapter 5 Instrument Fluid Pipeline........................................................................33
Chapter 6 Instrument Hardware Circuit....................................................................41
Chapter 7 Maintenance.......................................................................................56
Chapter 8 Analysis Method...................................................................................72
Chapter 9 Troubleshooting List..............................................................................84
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Summary of Contents for Dirui CS-T240

  • Page 1 Content List ..............................1 Chapter 1 Instrument specification ....................2 Chapter 2 Installation ........................9 Chapter 3 Performance and test flow ....................12 Chapter 4 Module Instruction ……………………………………………………………………..21 Chapter 5 Instrument Fluid Pipeline………………………………………………………………33 Chapter 6 Instrument Hardware Circuit…………………………………………………………..41 Chapter 7 Maintenance……………………………………………………………………………56 Chapter 8 Analysis Method………………………………………………………………………..72 Chapter 9 Troubleshooting List……………………………………………………………………84...
  • Page 2: Chapter 1 Instrument Specification

    Chapter Ⅰ Instrument Specification 1.1 CS-T240 Model Specification 1.1.1 Product Composition: The analytical part (host), operation part (computer system), the result output part, accessories and consumables. Product applicable scope: used for quantitative analysis of serum, plasma, urine, cerebrospinal fluid and other clinical chemical constituents of sample.
  • Page 3 ① ② 1.1.2 Front ① Model Logo ② Upper Cover ③ Front Cover ③ 1.1.3 Rear ① Purified Water Inlet ② Low Concentrate Waste Outlet ③ High Concentrate Waste Outlet ④ Detergent Inlet ⑤ Detergent Level Sensor Port ⑥ Left Back Cover ⑦...
  • Page 4 1.1.4 Working Table ① Reaction Cuvette Rinsing Unit ② Reaction Disk Unit ① ② ③ ④ ⑤ ⑥ ③ Reaction Tank Liquid Level Detection ④ Stirring Unit ⑤ Sample &Reagent Adding Unit ⑥ Sample & Reagent Disk ⑦ Mixer Rinsing Tank ⑧...
  • Page 5 1.2 Analysis Unit The working speed of CS-T240 auto-chemistry analyzer is 240 tests / hour at constant speed (single / double-reagent item), whose working period is 15 seconds. Instrument overall structure adopts the project of "2-disks + 1-probe + 1-stirring rod"—one reaction disk, one sample &...
  • Page 6 1.2.3 Probe and Stirring Unit Stirring Unit Probe Unit Probe unit:1 sample & reagent probe High-precision digital liquid detector Stirring Unit:1 High-speed hollow cup motor Surface high-intensity Teflon coating 1.2.4 Control unit1 ① Circuit panel boxes: 5 panels Order: from left to right ISE control panel(Optional)...
  • Page 7 ① ② ③ ④ 1.2.5 Control Unit 2 Semiconductor refrigeration system: ①+12V refrigerator power ②+5V control panel power ③Fan(15W/220W) ④Control panel(with status indication)...
  • Page 8 1.3 Function Overview Main work flow: 1. All mechanical moving parts initialization. 2. 3 times water blank measure is implemented after six time automatic rinsing 3. Sample reagent probe assimilates quantitive reagent when it descents to reagent sample disk after the sample reagent disk rotates to designated R1 reagent position. And then, sample reagent probe assimilates quantitive sample when it descents to reagent sample disk after the sample reagent disk rotates to designated sample position.
  • Page 9: Chapter 2 Installation

    Chapter 2 Installation 2.1 Space Requirement: To make sure the space of maintenance, operation and repair, please follow the instruction as below: ● Space between left (right) side of analyzer and the wall should ≥50cm ● Space between rear board of analyzer and the wall should ≥50cm ●...
  • Page 10 Please use air conditioner if the temperature or humidity can not meet the requirement above. The heat generated during working process by the instrument will be emitted at the rear of the instrument. Good ventilation should be kept well and ventilation equipment can be adopted if necessary, but direct air current should be avoided, or inaccuracy of instrument test may be caused.
  • Page 11 (e)Mechanical movement check Execute 20 times mechanical movement checks to make sure whether the washing block of rinsing mechanism nozzle abrases the reaction cuvette or not and each mechanism runs normally or not. (f)Rinse reaction cup+ ISE Select rinsing reaction cuvette in “Maintenance” interface, and execute rinsing reaction cuvette + ISE if ISE equipment is collocated.
  • Page 12: Chapter 3 Performance And Test Flow

    Chapter 3 Performance and Test Flow 3.1 Main Performance Index 3.1.1 Instrument standard specification Performance Index Standard Specification Grating rear spectrophotometry system, simultaneous photometric processing of 12 Wavelength range wavelength: 340 、 380 、 405 、 450 、 480 、 505 、...
  • Page 13 Reagent & sample disk with refrigerator, Sample & reagent disk,sample semiconductor cooling system.Total position, reagent position positions(21 routine samples, 45 reagent positions “CS-bacterial phosphate-free detergent”, 1 detergent position) Serum, plasma, Urine, cerebrospinal fluid, Sample type ascites and other body fluids Sample volume 3~35ul,0.1μl incremental Test tube Φ...
  • Page 14 type:code 128 Size: width: 8-12mm; alid length : within 40mm; start blank finish blank: within 3mm when cutting. Barcode information Sticking requirement:the lower edge of barcode should be within 15mm-20mm away from the test tube bottom to make sure right reading barcode,and make sure the barcode is aligned with sample position gap when putting the test...
  • Page 15 650VA power(VA) Water consumption 5L/小时 Installation power 220V/230V,50Hz/60Hz,1000VA requireme Systems storage temperature : 0℃~40℃, Using environment volatility: <±2℃/H; storage humidity:30%RH~ 80%R , non-condensing ; at working, temperature:15℃~30℃, volatility<±2℃/H; humidity:35%RH ~ atworking, relative 80%RH ,non-condensing ;not higher than 2000 meters above sea level. 3.1.2 Testing speed Test conditions Degree of reduced ability to process (estimated)
  • Page 16 3.2 Test Flow 3.2.1 典型测试流程...
  • Page 17 3.2.2 Test Flow Instruction 1. Periodic movement sequence of sample reagent probe a. Switch the pump to zero, internal and external wall rinsing b.Inhale 3ul air. c.Rotate to the position above the sample reagent disk d.Descend till the sample&reagent probe point into liquid level about 2mm e.
  • Page 18 120 个反应杯位置号 反应杯清洗机构 加 试 剂 与 加 反应盘复位点 样位置 搅拌位置 光电检测位 参考位置编号 figure 3-2 Position of reaction disk and probe Outer circle figure: No. of reaction cuvette;inner circle figure: No. of mechanism position. Reset point is in 71 position of reaction disk. Reagent1、2 and sample probe is in NO.1 position, stirring is in No.3 position.
  • Page 19 Reaction Cuvette Rinsing Movement Sequence Reaction disk rotate direction Test cell blank for 3 times(1 stop,2 pass) figure 3-3 Reaction cuvette rinsing probe position Above figure shows that 8 steps are needed when rinsing reaction cuvette. (3 times cell blank test is added) , therefore, to finish rinsing one reaction cuvette, 12 steps are needed:...
  • Page 21 Chapter 4. Module Introduction 4.1 Sample&reagent probe unit 4.1.1 Function introduction Sample&reagent probe can realize the assimilation from the sample test tube and reagent bottle and add into reaction cuvette. In addition, main function of reagent&sample probe component: liquid level detecting and collision detection in movement process, probe block detecting function.
  • Page 22 4.1.2 Composition Probe rotating arm Probe rotating gear belt Probe up-down light sensor block tablet Probe rotating step motor Probe up-down gear belt Probe up-down step motor Probe up-down weight Figure 4-1 probe components configuration Rotating motor mounting Rotating fixed light sensor hole position Up-down...
  • Page 23 Rotating probe drive ratio 12:34, using 0.9° stepper motor and 8 segment controller. Control accuracy can achieve 0.0398°. Up-down main gear driver diameter is 19.1mm, the 60mm for the perimeter, also using 0.9° stepper motor and 8 segment controller.Up-down control accuracy can achieve 0.01875mm. 4.2 Rotating mechanism unit 4.2.1 Function Introduction The main function of rotating mechanism is bearing of the sample warehouse,reagent warehouse...
  • Page 24 Driven gear Drive gear belt Driving step motor Code disk light sensor 4-5 sample &reagent disk motor driver configuration System transmission ratio is 10:1.Because of using 0.9 °step motor with 8 segment driver circuit, the wheel rotation accuracy can achieve 0.01125°. Leader of disk cover...
  • Page 25 temperature maintains at 6 degrees -10 degrees, 21 sample positions and 46 reagent positions (the 45th fixed position for placing phosphor-free CS-anti-bacterial detergent) 4.3.2 Refrigeration system configuration and installation Equipped with temperature-keeping reagent cooling storehouse 4-8 Reagent cooling storehouse and sample&reagent disk rack assembly configuration Barcode reader scanning window...
  • Page 26 Colorimetric cup Fixing pin rotating rack Driving gear Driven gear motor vibrator 4-10 reaction disk assembly configuration 120 colorimetric cup 120 teeth of code disk in colorimetric cup position 4-11 reaction cup installation disk assembly configuration(1) cuvette cuvette install pin Locking screw colorimetric cup rack...
  • Page 27 stirring rod rinsing bath Liquid level sensor probe rinsing bath rack Spilling outlet Optical window incubation bath Circular constant Circular constant temperature water temperature water outlet inlet 4-13 Incubation bath components configuration Phosphor-free Incubation bath detergent inlet 4-14 reaction disk with incubation bath components assembly configuration Optical system 4-15 reaction disk and optical system components assembly configuration...
  • Page 28 4.4 Stirring Unit Stir and mix reagent and sample after adding them。 Rotating mechanism of stirring and rotating arm adopts the direct drive way of step motor output axle, using 8 segment drive circuit of 0.9 ° motor, and the controlaccuracy can achieve 0.1125 °. The largest angle is limited by the open angle of rotatation code disk mechanical limit.
  • Page 29 Up-down linear sliding axle Up-down Up-down slider guide axle 4-18 stirring up-down slider drive configuration Because of mixing body movements are used by way of the crankshaft crank, so the movements of the positioning accuracy at different locations different. Landing and taking-off between the location of the speed of the highest, lowest accuracy, and precision at both ends of the highest, the lowest speed.
  • Page 30 Up-down slider Up-down slider 4-19 stirring mechanism up and down positions...
  • Page 31 4.6 Colorimetric cup rinsing mechanism Rinsing probe Colorimetric rack rinsing colorimetric cup probe Up-down slider Up-down step motor 4-20 Only one-dimensional movementavailable to colorimetric cup rinsing mechanism,and driving mechanism adopts the up-down driving mechanism of stirring rod. 4.7 Rack Rack includes the main rack, electrical, gas liquid valve and water tanks and other racks. Bottom-Board Main working table board Isolating...
  • Page 32 Electrical control refrigeration controller Switch Switch power 4-22...
  • Page 33: Chapter 5 Instrument Fluid Pipeline

    Chapter 5. Instrument Fluid Pipeline 5.1 Main function of liquid line The CS-T240 liquid line system can be divided into four parts: water inlet tank, 37 degrees centigrade temperature, colorimetric cup cleaning, the inner and outer arms and stirring rod cleaning.
  • Page 34 Liquid Line Principle 5.2.1 CS-T240 Liquid line principle sketch map...
  • Page 35 5.2.1 liquid line of water inlet tank Explanation: Z1——heater; Z2——lamp coller; Z3——float; Z4——temperature sensor; Z5——water tank; Z6——magnetic pump; Z7——water seven-pass;Z8——air discharger Z9——flow controller;Z10——pressure sensor;Z11——5 valve plate assembly;A——detergent; B——probe inner wall rinsing;C——Cuvette rinsing;D——stirring rod rinsing;E——probe external rinsing ; F——water inlet ; inlet ;...
  • Page 36 five-valve plate assembly, used for cuvette rinsing, stirring rinsing and external wall rinsing of the probe; second pass through the degassing device for the internal wall rinsing of the probe; third pass with the detergent used for the first probe rinsing of rinsing unit; fourth pass for the incubation bath; fifth pass access to pressure sensor for detecting the tank pressure;...
  • Page 37 controller. In order to improve the adjusting performance of the PID temperature controller in high temperature environment, the system is added the cooling device through the water tank to get a small amount of temperature cooled. 3, Turn off magnetic pump and temperature controller, open the drain valve SV10, time to turn off the drain valve when the incubation bath is draining.
  • Page 38 magnetic pump from the water tank and adjusted by voltage regulator to the stable pressure, about 0.45kgf/cm2. 2, vacuum pump, vacuum tank and pressure detector composes vacuum source with pressure value about -0.7kgf/cm2. Vacuum tank has the function of stable vacuum pressure. 3,Vacuum liquid discharging is high concentrate liquid –...
  • Page 39 Explanation:Z1——cuvette rinsing unit;Z2——detergent component;Z3——five-valve plate unit; Z4——vacuum pump unit;Z5——liquid collection vaccum tank unit;Z6——nozzle1;Z7——nozzle 2;Z8——nozzle 3;Z9——nozzle 4;A——water tank;B——iquid collection vaccum tank;C—— high concentrate waste outlet 5.2.5 ISE Part...
  • Page 41: Chapter 6 Instrument Hardware Circuit

    Chapter 6 Instrument Hardware Circuit 6.1 Hardware configuration main control board(including the control of AC ,pressure ) and temperature monitoring Stirring Reaction Sample Rinsing module module module module Figure 6-1 Hardware main frame...
  • Page 42 6.2 Security Note: At working, touching hardware panel with hand or any other objects is forbidden. In order to dismount panels, operation is only allowed when cut off power (220V, AC). 6.3 Circuit board and function list Function Description Circuit Name board No.
  • Page 43 Communication with the main control board ISE pump motor control Circuit board ISV1、ISV2、ISV3、ISV4 Magnetic valve control: ISE preamp board data collection 1. 37℃constant temperature heater control(200W) 2. Water tank heater control(200W) Solid relay board Gear pump control Water circulation pump Halogen lamp control refrigeration...
  • Page 44 2. Function As shown in above figure: Power input: ~220V L1、N1: AC power filter S1: power switch G1: power filter connecting with the ground of bottom-board P131: AC port of solid state relay board J501: fan port of AC system J502: AC power port of cooling system J503: power Supply Port of Halogen lamp...
  • Page 45 6.4.2 system Refrigeration 1. Wiring 2. Function Refrigeration system is composed of semiconductor refrigeration module, radiator, fan. The main function of refrigeration board is controlling the semiconductor refrigeration module, to keep the reagent disk temperature within 6-10℃. As shown in the figure : U1:refrigeration control board is the CPU of refrigeration...
  • Page 46 1.Wiring...
  • Page 47 2.Function Communicate with PC through serial port to transmit data, command and alarm information. Transmit data and command with reaction disk board, sample&reagent disk board, ISE board, refrigeration board and AD board communication through mother board. As shown in figure: Solenoid Valve: Refer to 5.2.1...
  • Page 48 6.4.4 Solid state relay board 1. Wiring 2. Function , Its main function is to control the solid state relay through main board to turn on and turn off the pump motor, heater and lamp. As shown in figure : Heater 200WA:water heater of incubation bath WATER TANK PUMP:water circulation pump of water tank GEAR PUMP:gear pump...
  • Page 49 6.4.5 Circuit board of reaction disk 1. Wiring 2. Function The main function of circuit board of reaction disk is to receive the command from the main control board, communicate with the main control board, transmit data and alarm command. Controlling sample&reagent unit includes sample&reagent disk rotation, probe unit running.
  • Page 50 6.4.6 Rinsing mixing circuit board 1. Wiring...
  • Page 51 2. Function The main function of rinsing mixing circuit board is to receive the command from the main control board, communicate with the main control board, transmit data and alarm command. Controlling sample&reagent unit includes sample&reagent disk rotation, probe unit running. Barcode scanning of sample&reagent disk As shown in figure:...
  • Page 52 6.4.7 Circuit board of sample&reagent disk 1. Wiring...
  • Page 53 2. Function Receive the command from the main control board, communicate with the main control board, transmit data and alarm command. Controlling sample&reagent unit includes sample&reagent disk rotation, probe unit running. Barcode scanning of sample&reagent disk As shown in figure : J041: signal port of sample&reagent disk optocoupler J042: signal inlet port of probe position limit optocoupler and liquid level detection optocoupler J043: signal inlet port of injection pump optocoupler...
  • Page 54 6.4.8 ISE circuit board 1. Wiring 2. Function...
  • Page 55 The instrument add calibrator into the reaction cuvette.The SIP injection pump aspirate the calibrator, and pump it into the Na、K、Cl electrode pipeline of the reaction tank. Test the electric potential on the basis of reference electrode. The sample probe aspirates sample and diluent, and pump it into the reaction cuvette.
  • Page 56: Chapter 7 Maintenance

    Chapter 7 Maintenance In order to ensure reliable system performance, excellent working status and span, please conduct system operation and regular maintenance strictly in accordance with the requirements in the service manual. Learning maintenance and overhaul of this chapter is also very important and in-depth study will enable the instrument to achieve the best running status and exert the best performance.
  • Page 57 Acidic high concentrate detergent: 0.1mol / l hydrochloric acid; alkaline high concentrate detergent: 0.5% (V / V) sodium hypochlorite. Please use the high concentrate detergent designated by Dirui. If undesignated types of high concentrate detergent are used, inappropriate analysis results might be received.
  • Page 58 The injection pump can be seen in figure. Observe whether the injection pump is leaking. If so, check the leakage causes, and check the pipeline and connector timely. 7.2.2 Check/rinse sample & reagent probe In online status, click “Instrument resetting” in “Maintenance”, and instrument executes resetting.
  • Page 59 When rinsing, carefully observe rinsing probe working and whether probe infusing is normal and assimilating is completely. If infusing abnormal, check pressure value of water infusing pressure gauge If assimilating abnormal, check pressure value of assimilating vacuum 7.2.5 Check waste connection and discharging Biological contamination danger: During waste water operation, please put on gloves, put on work clothes and if necessary, wear protective glasses.
  • Page 60 Make sure the analysis part power supply is switched off. Lift the rotating arms of samle&reagent probe by hands to the top position, and rotate it to the top of sample&reagent storehouse for convenient operation. Caution: When cleaning, do not touch directly the probe surface to prevent probe scratch;...
  • Page 61 Switch on the power of analysis part and wait 30 seconds, enter the "maintenance - routine maintenance" column to implement “instrument resetting", the system will automatically reset the sample&reagent probe and rinse them with deionized water. 7.3.2 Rinse stirring rod Biological contamination danger: In operation, please put on gloves, work cloths, and put on protective glasses for the best.
  • Page 62 Switch on the power of analysis part and wait 30 seconds, enter the "maintenance - routine maintenance" column to implement “instrument resetting", the system will automatically reset the sample&reagent probe and rinse them with deionized water. 7.3.3 Sample/ barcode window rinsing Caution: Do not gaze scanning laser light, or it may cause eyes injury Make sure the analysis part power is switched off.
  • Page 63 Biological contamination danger: In operation, please put on gloves, work cloths, and put on protective glasses for the best. Do not dispose the gauze used to clean sample&reagent probe.rinsing bath at your own will, please follow the relevant provisions for proper disposal. Make sure the analysis part power is switched off.
  • Page 64 2000 hours, the light source lamp should be checked. Caution: Please use consumables recommended by DIRUI company, using other consumables may cause system performance degradation. Do not touch the light source lamp shell surface and lens in front of the light source lamp by hand, because it may change the characteristics of the light source.
  • Page 65 Loosen the two screws fixing light source seat to remove halogen lamp. Mount a new halogen lamp according to the above opposite steps; pay attention to tighten the screws. The cooling rubber hose in the lamp room can not be twisted and down-lead can not be loosed or cocked.
  • Page 66 Make sure the analysis part power is switched off. Remove sample&reagent disk cover and then sample&reagent disk Lift the rotating arm of sample&reagent probe by hands to its top position, and rotate its rotating arm to keep sample&reagent probe away from rinsing bath for convenient operation.
  • Page 67 Note: Sample&reagent probe is precisely processed to ensure the sample reagent adding precision. If the probe point is damaged or bent, checking sample&reagent probe is a must, or no guarantee can be made for test precision. 7.7.2 Clean sample&reagent probe Warning:...
  • Page 68 Caution: Sample&reagent probe is precisely processed to ensure the sample and reagent adding precision. If the probe point is damaged or bent, checking sample reagent probe is a must, or no guarantee can be made for test precision. 7.7.4 Rinse sample&reagent probe When it is found that the water level of rinsing bath is too high when rinsing sample&reagent probe because of no discharging available, which may be caused by the blocked leaking hole.
  • Page 69 Please deal with removed stirring rod properly. Caution: Please use consumables recommended by DIRUI company, using other consumables may cause system performance degradation. Make sure the analysis part power is switched off. Prepare a new stirring rod and wipe the flat part of it with gauze or cotton stick dipped with cleaning liquid, and then wipe it with gauze dipped with deionized water.
  • Page 70 In operation, please put on gloves, work cloths, and put on protective glasses for the best Please deal with removed reaction cuvette properly which is broken. Caution: Please use consumables recommended by DIRUI company, using other consumables may cause system performance degradation. Make sure the analysis part power is switched off.
  • Page 71 Put on protective gloves to remove fixing screws. Rotate reaction disk by hand and remove the reaction cuvette sequently. Take out reaction cuvette while rotating it. Rinse the new reaction cuvette dipped in with water; rinse inside and outside of reaction cuvette and no scratch is allowed. Rotate reaction disk by hands, and mount new reaction cuvette on reaction disk and check the six sets reaction cuvette simultaneously.
  • Page 72: Chapter 8 Analysis Method

    Select and execute “cuvette blank test” after click “System maintenance”, and observe execution result and reaction status. Chapter 8 Analysis Method CS-T240 adopts three analysis methods as follows: End point analysis method Two-points analysis method Rate analysis method Analysis principle The analysis principle of auto-chemistry analyzer is based on the Beer-Lambert law that material absorpts light selectively.
  • Page 73 If the sample liquid adequate distribution, interaction between liquid and incidence monochromatic light only happens during absorbing process. No fluorescence, disperse and photochemical appear. No interaction between substances in the solution while absorbing process. The absorbency possess conducts nature, and this condition conforms to the Beer-Lambert law Types of analysis methods Method Photometry point...
  • Page 74 Fist half 1 − L– 0 – 0 – 0 1<L≤M<N≤49 3-points double items Second half − − − M – N – 0 – 0 1<L≤M<N≤49 Fist half L– M – 0 - 0 △A(M-L) 3≤L<M<N<P≤49 Rate B double L +2<M items analysis...
  • Page 75 L,m,n,p,q,r : Photometric points :Volume of the reagent, n=1 to 4 B B B :Through cell blank 、、 (B1,B2,B3 )/3 :Average value of three times passing through cell blanks :Absorbance at photometric point x △A(m-L) :Change in absorbance per minute between photometric points L and M :Liquid volume correction factor ∑...
  • Page 76 Cell blank Time Example 1:TBIL-Total bilirubin reagent kit (Surfactant / diazonium salt method) 、 wavelength Absorbance Main 550nm,sub 660nm 0~2A; range test mode Endpoint analysis method Optical path 10mm reagent sample 10uL 1:250uL Single Mixing reagent A:B=50:1 reagent storage temperature 37℃...
  • Page 77 conversion Adult;5.1~19umol/L(0.3~1.1mg/dL) Reference Newly born:20~200umol/L(1.2~12mg/dL) value Example 2、UA(uric acid)-Uric acid liquid reagent kit 520nm ( 500,550 wavelength Main Absorbance 0~2A optional) range Test mode Endpoint analysis method Optical path 10mm Reagent 1:200uL;2:50uL sample Single 4 parts of reagent 1 part of Mixing storage 2~8℃...
  • Page 78 Two points end point analysis two points rate analysis Rate Analysis Rate analysis, also known as zero-class dynamics analysis, refers to the reaction rate is directly proportional to the zero power of substrate concentration, which has nothing to do with the substrate concentration. Hence, the reactants can generate a certain product at constant speed throughout the reaction process, resulting in even decrease or increase of absorbance of measured solution at a wavelength, and the decrease or increase speed (△...
  • Page 79 Example 3:ALT/GPT - Alanine aminotransferase (IFCC) wavelength Main 340nm Absorbance range 0~2A Test mode Rate assay Optical path 10mm Reagent sample 15uL 1:240uL;2:60uL Single reagent 4 reagent 1and 1reagent 2 Mixing storage 2~8℃ 5days stability temperature 37℃ incubation 5min reaction 60s delay,...
  • Page 80 Principle of electrolyte measurement 8.6.1 Principle Internal standard solution is firstly added into the instrument reaction cuvettes, and assimilates it and discharges it into the Na, K, and Cl electrode solution line through the SIP injection pump to measure its electrode potential which is relative to reference electrode potential.
  • Page 81 T: absolute temperature (t ℃ +273.15) (K) F: Faraday constant (9.6485309 × 104 C × mol-1) ai : Ion (i) activity f: activity coefficient Ci: concentration n: a given ion (i) the charge number (Cation is positive, anion is negative) 8.6.3 Test method Working curve preparation, the internal standard solution concentration...
  • Page 82 C (IS): the concentration of internal standard solution E (IS): the potential of internal standard solution 8.6.3.3 concentration calculation The calculation of routine sample, STAT sample, and concentration of quality control liquid is based on the concentration of internal standard solution. Internal standard solution is different with the different sample.
  • Page 83 Diluent volume 600ul Processing 100samples/h(only measuring electrolyte) ability Na + 20 ~ 200mmol / L (when only serum ) 10 ~ 400mmol / L (when measuring urine) K + 1.0 ~ 15.0mmol / L (when only serum) Measuring 1 ~ 200mmol / L (when measuring urine) range Cl-20 ~ 200mmol / L (when only serum) 10 ~ 400mmol / L (when measuring urine)
  • Page 84: Chapter 9 Troubleshooting List

    Detailed Description Solution Code description  In the CS-T240 software, enter "system maintenance" interface after getting on-line, implement "mechanical movement check", and observe the stirring rod running status Malfunction 1:stirring mechanism doesn’t move Solution: 1, Check stirring mechanism up-down movements, mechanical repair is required if resistance is big.
  • Page 85 If the potential is not normal, check the coupler Malfunction 3:Stirring mechanism cannot reach the zero position. solution:Mechanical repair  In the CS-T240 software, enter into the "system maintenance" interface after on-line, implement "mechanical movement check", and observe the stirring rod running status Malfunction 1:stirring mechanism stops solution:...
  • Page 86 mechanical repair is required if resistance is big. 2, check whether both ends of connector of the motor are connected well. 3, check whether the conductivity of motor lead wire is good. 4, check the motor drive module of circuit board is working normally 5、Check whether the it is at top position, check whether up-down zero light sensor works normally at top position.
  • Page 87  In CS-T240 software, enter into the "system maintenance" interface after on-line, implement "mechanical movement check", and observe the stirring rod running status Malfunction 1:stirring mechanism doesn’t move Solution: 1, Check stirring mechanism up-down movements, mechanical repair is required if resistance is big.
  • Page 88 When resetting, Stirring Stirring mechanism mechanism The solution is the same as 1-4 abnormal failed to reach rinsing bath.  Enter into system maintenance window and execute “Mechanical movement check”. Observe the running When status of stirring rod. resetting, Malfunction 1:stirring mechanism doesn’t move Stirring Stirring mechanism...
  • Page 89 Malfunction 2:Rinsing mechanism can reach the top, but can not check the zero position. solution:Manually make rinsing mechanism repeatedly rise to peak, transferred the multimeter To DC, plug the negative point of the first three core P02, P02 plugs are 2 pole core.
  • Page 90 Malfunction 2:Rinsing mechanism of reaction cup cannot reach the top solution:the same to that of malfunction 2 in 3-1...
  • Page 91 9.3 Reaction disk malfunction analysis Alarm Detailed Description Solution Code description Malfunction:The quantity counted by reaction cup light sensor does not conform to real quantity of cup rotated. solution: Check whether the signal status of counting light sensor is normal. Reaction disk fails Reaction to rotate to the...
  • Page 92 Sample probe mechanism malfunction analysis Alarm Detailed Description Solution Code description Enter system maintenance window, execute "mechanical inspection." Observe running probe arm Malfunction 1: no liquid in sample cup Sample Solution: probe (1) confirm whether there are samples left int the sample Sample abnormal.
  • Page 93 can run, but the photo sensor cannot detect any signal Solution: Manually make the probe body go up to the peak repeatedly, transfer the multimeter to DC, negative points the first 6-core plugs P042, P042 positive pole core plugs 5. 1, multimeter readings between the positive changes from 5V to 0, indicating photo sensor and lead are normal (1) Check conductors...
  • Page 94 4, check the sample&reagent motor drive circuit board Malfunction 2: probe arm to the coupler's position can run, but the photo sensor cannot detect any signal Solution: 1, multimeter readings between the positive changes from 5V to 0, indicating photo sensor and lead are normal (1) Check conductors Check whether the...
  • Page 95 When there’s touch, the photo sensor blocks, and J7-2touch is high; When there’s no touch, the photo sensor is not blocked, J7-2touch is low. Enter CS-T240 operation system, system maintenance When window, execute "mechanical movement". Observe Sample&reage sample&reagent probe running status.
  • Page 96 If the potential is not normal, check photo sensor. Malfunction 3: Couldn’t sway to reaction disk position Solution: mechanical repair Enter CS-T240 operation system, system maintenance When the window, execute "mechanical movement". Observe probe Sample&reage running status.
  • Page 97 Solution:same as to 8-5 When Sample&reage Reagent probe probe Probe couldn’t rise to the peak, solution same as to 8-1. abnormal rotates, it deciates from the peak Malfunction: When there is liquid in the reagent bottle, Sample&reagent probe cannot detect it Solution: it couldn’t 1, Check the liquid level detection reagent with the sample...
  • Page 98 location well 3, Check the conductivity of the electrical conductor lead is good 4, Check sample&reagent circuit board’s motor drive circuit board kit part Malfunction 2: When sample&reagent disc rotates to the specified location, the sample&reagent disk does not stop at the specified position, or when turns to specified location, the photo sensor couldn’t detect it, or the signal of the photo sensor is not correct...
  • Page 99 well of this mechanism 3, Check whether the conductivity of the conductor’s lead are good 4, Check the circuit board of the sample reagent disk Malfunction 2: Sample reagent disk rotates, back couldn’t find the position of back to zero Solution: Manually rotate the sample&reagent disk, transfer the multimeter to DC, negative points P041 core plug No.3, positive points P041 core plug No.2...
  • Page 100 9.12 Injection pump malfunction analysis Alarm Detailed Description Solution Code description Malfunction 1: Syringe pump doesn’t move or it couldn’t rise up to the top Solution: 1. When the instrument is working, the syringe pump doesn’t work Syringe pump Syringe pump 14-1 doesn’t rise up abnormal...
  • Page 101 9.14 Incubation bath malfunction analysis Alarm Detailed Description Solution Code description (1) Make sure the cooling fan of the instrument is rotating normally (2) Check the temperature sensor is working normally temperature of Incubation bath incubation 20-1 temperature (3) Check the connector part of the temperature sensor on bath is above abnormal the main control board...
  • Page 102 result. Calibration (1)Please check whether the Calibrator is over measuring ISE is over volume, reagent volume is adequate, and 0-33 Note measuring lower limit, whether the position is correct lower limit the current Calibration has no result. Calibration is over (1)Please check whether the Calibrator measuring ISE is over...
  • Page 103 volume) remaining volume of (1)add ISE internal standard solution internal ISE internal standard (2)Enter into system maintenance, and standard solution is 38-1 Note execute "ISE internal standard solution liquid is inadequate reagent pipeline rinsing” inadequate (less then the user (3)Execute ISE calibration designed volume) remaining...
  • Page 104 potential of (2) please refer to " electrolyte device internal maintenance " in the "user manual" for standard detail. solution, the average value (EAV)of three potentials is over the following range (internal standard solution) K: -90.0mv≤E AV≤-10mv In the 5-measurin g point potential of internal...
  • Page 105 solution, the detail. difference (FI) of maximum minimum is within following range Na: 0.7mv≤|FIV (2)-FIV (4)| In the 5-measurin g point potential of internal standard solution, the difference (FIV) of (1) Enter into system maintenance, and execute "ISE check." maximum Note ISE Noise 61-2 error...
  • Page 106 maximum minimum is within following range (internal standard, sample) Cl: 0.8mv≤|FIV (2)-FIV (4)| The slope value of calibration result is within following range or the impact of electrode is (1) Enter into system maintenance, and execute "ISE check." (cross-conta mination 62-1 Note Prepare...
  • Page 107 following K:(1) 32.0mv≤SL OPE≤37mv or68.1mv≤S LOPE The slope value of calibration result is within following range or the impact of (1) Enter into system maintenance, and electrode is execute "ISE check." (cross-conta 62-3 Note Prepare mination (2) please refer to " electrolyte device abnormal rate (A) is maintenance "...
  • Page 108 calibration result is not updated (1)The slope value calibration result is within following range or the (1) Enter into system maintenance, and impact of execute "ISE check." electrode is slope (cross-conta value (2) please refer to " electrolyte device 63-2 Note mination (SLOPE)...
  • Page 109 calibration result is not updated (1)the concentratio n of internal solution(C (IS))is within (1) Enter into system maintenance, and following execute "ISE check." range :Na: concentration (2) please refer to " electrolyte device C(IS)< 64-1 Note of internal maintenance " in the "user manual" for 120.0mmol/ solution detail.
  • Page 110 (IS))is abnormity maintenance " in the "user manual" for detail. within following range Cl:C (3) Please re-execute ISE calibration. (IS)< 80.0mmol/l 140.0mmol/ <C(IS) (2) current calibration result is not updated Because of implementa tion of the maintenanc e (ISE ISE required cleaning, Executive ISE calibration calibration...
  • Page 111 Alarm Detailed Description Solution Code description Malfunction 1: pump stops or fails to reach the top solution: observe running status of pump: 1.when working, injection pump is not running. (1) Check whether both ends of connector of the motor are connected well. (2) Check whether the conductivity of motor lead wire is good.
  • Page 112 Alarm Detailed Description Solution Code description Malfunction 1: injection pump stops or fails to leave the top solution: observe running status of pump 1.when working, injection pump is not running. (1) Check whether both ends of connector of the motor are connected well. (2) Check whether the conductivity of motor lead wire is good.
  • Page 113 Alar Detailed Description Solution description Code Malfunction 1: Motor stops or fails to leave the top (1) Check whether both ends of connector of the motor are connected well. (2) Check whether the conductivity of the motor lead wire is good. (3)check motor drive circuit of sample reagent disk circuit board (4) Inspect whether the rise and fall zero light sensor...
  • Page 114 Check voltage between P142A plug pin 1,3, if the voltage is not 5v, check the connection of plug. If the voltage is 5v, check potential of P142 plug pin 2. The potential is high when reaching to the top position, otherwise the potential is low.
  • Page 115 (2)Check the light sensor input circuit of sample reagent disk circuit board. 2. The value of multimeter is not changing. (1)Check the wire Check whether the 1.2.3 core of P142 plug is connected well, and whether the two ends connected well.
  • Page 116 failure Reaction disk detects Reaction disk malfunction Lower machine monitoring software monitors error reset failure when resetting, information, and analyze the reaction disk Reaction disk board-related failure to eliminate the error reset failure Sample& reagent disk Use lower machine monitoring software to monitor detects Sample&...
  • Page 117 Check whether the liquid level detector of reaction bath is clean, whether there is water in reaction bath, Reaction bath Reaction bath whether water level reaches the position where the liquid level liquid level detector can detect, and check liquid level detector malfunction malfunction in reaction bath is working normally;...
  • Page 118 information of reaction disk to debug. Check whether waste liquid bottle is full; check Waste liquid Waste liquid whether the sensor in waste liquid bottle is normal; bottle full bottle full check whether relevant electrical units of the floater in waste liquid bottle and control board are normal. Switch Check whether the floaters of high and low liquid malfunction,...
  • Page 119 ISE malfunction Observe whether there is ISE alarm information; when testing, Use lower machine monitoring software to monitor malfunction in follow-up ISE error information, and refer to the related error testing stops information of ISE board to debug. Check gear pump and pressure sensor of sample pipeline are normal.
  • Page 120 Check whether light source, water quality of Continuous incubation bath, reaction cup and counting light Continuous emergence of 5 sensor of reaction disk are normal; test data emergence of dirty cups collecting board lines by using the testing program dirty cups of data collecting board.
  • Page 121 (1)observe whether current value displayed on digital pipe of refrigeration system is normal. refrigeration refrigeration system current 144-2 (2)Make sure which current is abnormal, and cope abnormal abnormal with it after check the corresponding abnormal Peltier and circuit board. (1)observe which chip is abnormal refrigeration refrigeration system...
  • Page 122 malfunction line AD collector over normal range The 2nd line Check the AD collection board and preamp board AD collector measuring malfunction value of the 146-2 2nd line AD collector over normal range The 3rd line Check the AD collection board and preamp board AD collector measuring malfunction...
  • Page 123 The seventh Check the AD collection board and preamp board line measuring collector value of the 146-7 malfunction seventh line AD collector over normal range eighth Check the AD collection board and preamp board line measuring collector value of the 146-8 malfunction eighth...
  • Page 124 over normal range...

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