Aqua Lytic SpectroDirect AL800 Instruction Manual
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Aqua Lytic SpectroDirect AL800 Instruction Manual

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Summary of Contents for Aqua Lytic SpectroDirect AL800

  • Page 1 Spectrophotometer AL800/ SpectroDirect Instruction Manual...
  • Page 3 Important steps before using the photometer Please carry out the following steps as described in the Instruction manual. Become familiar with your new pho- tometer before starting with the first tests: • Unpacking and inspection of delivery contents, see page 352. IMPORTANT NOTE: Before using the SpectroDirect photometer it is necessary to insert the two batteries.
  • Page 5 Wichtige Information Um die Qualität unserer Umwelt zu erhalten, beschützen und zu verbessern Entsorgung von elektronischen Geräten in der Europäischen Union Aufgrund der Europäischen Verordnung 2012/19/EU darf Ihr elektronisches Gerät nicht mit dem normalen Hausmüll entsorgt werden! Tintometer GmbH entsorgt ihr elektrisches Gerät auf eine professionelle und für die Umwelt verantwortungsvolle Weise.
  • Page 6 Información Importante Para preservar, proteger y mejorar la calidad del medio ambiente Eliminación de equipos eléctricos en la Unión Europea Con motivo de la Directiva Europea 2012/19/UE, ¡ningún instrumento eléctrico deberá eliminarse junto con los residuos domésticos diarios! Tintometer GmbH se encargará de dichos instrumentos eléctricos de una manera profesional y sin dañar el medio ambiente.
  • Page 7 Wichtiger Entsorgungshinweis zu Batterien und Akkus Jeder Verbraucher ist aufgrund der Batterieverordnung (Richtlinie 2006/66/ EG) gesetzlich zur Rückgabe aller ge- und verbrauchten Batterien bzw. Akkus verpflichtet. Die Entsorgung über den Hausmüll ist verboten. Da auch bei Produkten aus unserem Sortiment Batterien und Akkus im Lieferumgang enthalten sind, weisen wir Sie auf folgendes hin: Verbrauchte Batterien und Akkus gehören nicht in den Hausmüll, sondern können unentgeltlich bei den öffentlichen Sammelstellen Ihrer Gemeinde und überall...
  • Page 8 Indicación importante acerca de la eliminación de pilas y acumuladores Basado en la norma relativa a pilas/ baterías (directiva 2006/66/CE), cada consumidor, está obligado por ley, a la devolución de todas las pilas/ baterías y acumuladores usados y consumidos. Está prohibida la eliminación en la basura doméstica. Ya que en productos de nuestra gama, también se incluyen en el suministro pilas y acumuladores, le sugerimos lo siguiente: Las pilas y acumuladores usados no pertenecen a la basura doméstica, sino que pueden...

  • Page 9: Safety Precautions

    Safety precautions CAUTION Reagents are formulated exclusively for chemical analysis and must not be used for any other purpose. Reagents must not get into the hands of children. Some of the reagents contain substances which are not entirely harmless environmentally. Be aware of the ingredients and take proper care when disposing of the test solution.

  • Page 10: Table Of Contents

    Table of contents Part 1 Methods ....................9 1.1 Table of Methods ...................... 10 Acid demand to pH 4.3 ....................16 Alkalinity-p (p-Value) ....................... 18 Alkalinity-total (Alkalinity-m, m-Value) ................20 Alkalinity-total HR (Alkalinity-m HR, m-Value HR) ............22 Aluminium with tablets ....................24 Aluminium (Powder Pack) ....................
  • Page 11 Chlorine with liquid reagent 24 mm ................70 free Chlorine ....................... 70 total Chlorine ......................71 differentiated determination (free, combined, total) ............ 72 Chlorine (Powder Pack) 24 mm ..................74 free Chlorine ....................... 74 total Chlorine ......................75 differentiated determination (free, combined, total) ............ 76 Chlorine MR (VARIO Powder Pack) ..................
  • Page 12 DEHA (Powder Pack) ..................... 130 Fluoride ........................132 Formaldehyde, 10 mm ....................134 Formaldehyde, 50 mm ....................136 Formaldehyde, 16 mm ....................138 Hardness, total ......................140 Hardness, total HR ......................142 Hydrazine ........................144 Hydrazine with liquid reagent ..................146 Hydrogen peroxide .......................
  • Page 13 Oxygen, active ......................210 Ozone ........................... 212 in presence of Chlorine ..................... 214 in absence of Chlorine ....................216 in presence of Chlorine ..................... 218 in absence of Chlorine ....................220 pH value with tablet ...................... 222 pH value with liquid reagent ..................224 Phenol ..........................
  • Page 14 Important notes ................... 290 1.2.1 Correct use of reagents ................290 1.2.2 Cleaning of vials and accessories for analysis ..........291 1.2.3 Guidelines for photometric measurements ........... 292 1.2.4 Sample dilution techniques ................293 1.2.5 Correcting for volume additions ..............293 Part 2 Instrument Manual ...............
  • Page 15 Absorption / Transmission ................327 Spectrum (Scan) ................... 328 Kinetic ......................330 2.4.7 User operations .................... 334 User method list ................... 334 User Concentration Methods ................ 336 User Polynomials ..................338 Delete User Methods ..................341 Print Data of User Methods ................342 Initialise User Method System ...............
  • Page 16 AL 800 / SpectroDirect_8 05/2017...

  • Page 17: Part 1 Methods

    Part 1 Methods AL 800 / SpectroDirect_8 05/2017...

  • Page 18: Table Of Methods

    1.1 Table of Methods No. Analysis Reagent Range Displayed Method Page [nm] 20 Acid demand tablet 0.1-4 mmol/l Acid/Indicator 615 16 1,2,5 to pH 4.3 T 35 Alkalinity-p T tablet 5-300 mg/l Acid /Indicator 551 18 1, 2, 5 CaCO 30 Alkalinity, total T tablet 5-200 mg/l...
  • Page 19 No. Analysis Reagent Range Displayed Method Page [nm] 105 Chlorine HR tablet 5-200 mg/l Cl KI/Acid 470 82 (Kl) T 119 Chlorine tablet 0.05-1 mg/l ClO DPD, Glycine 510 84 1, 2 dioxide 50 T 120 Chlorine tablet 0.05-2.5 mg/l ClO DPD, Glycine 510 86 1, 2...
  • Page 20 No. Analysis Reagent Range Displayed Method Page [nm] 206 Hydrazine L liquid 0.005-0.6 mg/l N 4-(Dimethylamino)- 455 146 benzaldehyde 209 Hydrogen tablet 0.01-0.5 mg/l H DPD/Catalyst peroxide 50 T 210 Hydrogen tablet 0.03-1.5 mg/l H DPD/Catalyst peroxide T 215 Iodine T tablet 0.05-3.6 mg/l I...
  • Page 21 No. Analysis Reagent Range Displayed Method Page [nm] 270 Nitrite T tablet 0.01-0.5 mg/l N N-(1-Naphthyl)- ethylendiamine 2, 3 272 Nitrite PP powder pack 0.01-0.3 mg/l N Diazotization 275 Nitrite LR TT tube test 0.03-0.6 mg/l N Sulfanilic acid/ Naphthylamine 276 Nitrite HR TT tube test 0.3-3...
  • Page 22 No. Analysis Reagent Range Displayed Method Page [nm] 316 Phosphonate powder pack 0-125 mg/l P Persulfate 890 248 UV-Oxidation 340 Potassium T tablet 1-10 mg/l K Tetraphenylborate- 730 252 Turbidity 345 S Abs 436 nm direct 0-50 EN ISO 436 254 (Colour) reading 7887:1994...
  • Page 23 1.1 Methods The precision of Lovibond Reagent Systems (tablets, powder packs and tube tests) is identical ® to the precision specified in standards literature such as American Standards (AWWA), ISO etc. Most of the data referred to in these standard methods relates to Standard Solutions. Therefore they are not readily applicable to drinking-, boiler- or waste-water, since various interferences can have a major influence on the accuracy of the method.

  • Page 24: Acid Demand To Ph 4.3

    1.1 Methods Acid demand to pH 4.3 with Tablet 0.1 – 4 mmol/l 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure marks are aligned.
  • Page 25 1.1 Methods Notes: 1. The terms total Alkalinity, Alkalinity-m, m-Value and Acid demand to pH 4.3 are identical. 2. For accurate results exactly 10 ml of water sample must be taken for the test. Reagent Form of reagent/Quantity Order-No. ALKA-M-PHOTOMETER Tablet / 100 4513210BT AL 800 / SpectroDirect_8 05/2017...

  • Page 26: Alkalinity-P (P-Value)

    1.1 Methods Alkalinity-p = p-value with Tablet 5 – 300 mg/l CaCO 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 27 1.1 Methods Notes 1. The terms Alkalinity-p, p-Value and Alkalinity to pH 8.2 are identical. 2. For accurate test results exactly 10 ml of water sample must be taken for the test. 3. This method was developed from a volumetric procedure for the determination of Alkalinity-p.

  • Page 28: Alkalinity-Total (Alkalinity-M, M-Value)

    1.1 Methods Alkalinity, total = Alkalinity-m = m-Value with Tablet 5 – 200 mg/l CaCO 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 29 1.1 Methods Notes: 1. The terms total Alkalinity, Alkalinity-m, m-Value and Alkalinity to pH 4.3 are identical. 2. For accurate results exactly 10 ml of water sample must be taken for the test. 3. Conversion table: Acid demand to pH 4.3 German English French...

  • Page 30: Alkalinity-Total Hr (Alkalinity-M Hr, M-Value Hr)

    1.1 Methods Alkalinity HR, total = Alkalinity-m HR = m-Value HR with Tablet 5 – 500 mg/l CaCO 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 31 1.1 Methods Notes: 1. For verification of the result look carefully at the bottom of the vial. If a thin yellow layer forms, then mix the vial again. This ensures that reaction is complete. Reread the result. 2. Conversion table: Acid demand to pH 4.3 German English...

  • Page 32: Aluminium With Tablets

    1.1 Methods Aluminium with Tablet 0.01 – 0.3 mg/l Al 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 33 1.1 Methods Notes: 1. Before use, clean the vials and the accessories with Hydrochloric acid (approx. 20%). Rinse them thoroughly with deionised water. 2. To get accurate results the sample temperature must be between 20°C and 25°C. 3. A low test result may be given in the presence of Fluorides and Polyphosphates. The effect of this is generally insignificant unless the water has fluoride added artificially.

  • Page 34: Aluminium (Powder Pack)

    1.1 Methods Aluminium with Vario Powder Pack 0.01 – 0.25 mg/l Al Use two clean vials (24 mm Ø) and mark one as blank for zeroing. 1. Fill 20 ml of water sample in a 100 ml beaker. 2. Add the contents of one Vario Aluminum ECR F20 Powder Pack straight from the foil to the water sample.
  • Page 35 1.1 Methods 12. Place the vial (the blank) in the sample chamber making sure that the marks are aligned. prepare Zero 13. Press ZERO key. press ZERO 14. Remove the vial from the sample chamber. 15. Place the vial (the sample) in the sample chamber making sure that the marks are aligned.

  • Page 36: Ammonia With Tablet

    1.1 Methods Ammonia with Tablet 0.02 – 1 mg/l N 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 37 1.1 Methods Notes: 1. The tablets must be added in the correct sequence. 2. The AMMONIA No. 1 tablet will only dissolve completely after the AMMONIA No. 2 tablet has been added. 3. The temperature of the sample is important for full colour development. At a temperature below 20°C the reaction period is 15 minutes.

  • Page 38: Ammonia (Powder Pack)

    1.1 Methods Ammonia with Vario Powder Pack 0.01 – 0.8 mg/l N Use two clean vials (24 mm Ø) and mark one as blank for zeroing. 1. Fill a clean vial (24 mm Ø) with 10 ml of deionised water (this is the blank). 2.
  • Page 39 1.1 Methods Notes: 1. Extremely basic or acidic water samples should be adjusted with 0.5 mol/l (1 N) Sulfuric acid solution or 1 mol/l (1 N) Sodium hydroxide solution to pH 7. 2. Interferences: Interfering substance Interference levels and treatments Calcium greater than 1000 mg/l CaCO Iron...

  • Page 40: Ammonia, Low Range (Lr)

    1.1 Methods Ammonia LR with Vario Tube Test 0.02 – 2.5 mg/l N 1. Open one white capped reaction vial and add 2 ml deionised water (this is the blank). 2. Open another white capped reaction vial and add Ø 16 mm 2 ml water sample (this is the sample).
  • Page 41 1.1 Methods Notes: 1. Strong alkaline or acidic water samples must be adjusted to approx. pH 7 before analysis (use 1 mol/l Hydrochloric acid resp. 1 mol/l Sodium hydroxide). 2. Iron interferes with the test. The interferences will be eliminated as follows: Determine the amount of total iron present in the water sample.

  • Page 42: Ammonia, High Range (Hr)

    1.1 Methods Ammonia HR with Vario Tube Test 1 – 50 mg/l N 1. Open one white capped reaction vial and add 0.1 ml deionised water (this is the blank). 2. Open another white capped reaction vial and add Ø 16 mm 0.1 ml of water sample (this is the sample).
  • Page 43 1.1 Methods Notes: 1. Strong alkaline or acidic water samples must be adjusted to approx. pH 7 before analysis (use 1 mol/l Hydrochloric acid resp. 1 mol/l Sodium hydroxide). 2. If chlorine is known to be present, add one drop of 0.1 mol/l Sodium thiosulfate for each 0.3 mg/l Cl in a one litre water sample.

  • Page 44: Arsenic

    1.1 Methods Arsenic 0.02 – 0.6 mg/l As Reagents (note 2): • 40 % Sulfuric Acid (H ) p.a. • Dissolve 8.33 g Potassium Iodide (KI) p.a. in 50 ml of deionised water Note: stored in a dark bottle it can be used for 1 week •...
  • Page 45 1.1 Methods Sample preparation: Reaction times must be exactly kept! 1. Prepare the dry reaction apparatus (note 4) and place it under a fume hood (toxic fumes!). 2. Pipette 50 ml of water sample into a 100 ml Erlen- meyer flask (NS 29/32). 3.
  • Page 46 1.1 Methods Notes: 1. Appropriate safety precautions and a good lab technique must be used during the whole procedure. 2. Reagents are commercially and should ordered locally. MSDS: please refer to your local reagent supplier. Ensure proper disposal of reagent solution.
  • Page 47 AL 800 / SpectroDirect_8 05/2017...

  • Page 48: Boron

    1.1 Methods Boron with Tablet 0.1 – 2 mg/l B 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 49 1.1 Methods Notes: 1. The tablets must added in the correct sequence. 2. The sample solution should have a pH value between 6 and 7. 3. Interferences are prevented by the presence of EDTA in the tablets. 4. The rate of colour development depends on the temperature. The temperature of the sample must be 20°C ±...

  • Page 50: Bromine

    1.1 Methods Bromine with Tablet 0.1 – 3 mg/l Br 1. Fill a clean 10 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.
  • Page 51 1.1 Methods Notes: 1. Vial cleaning: As many household cleaners (e.g. dishwater detergent) contain reducing substances, the subsequent determination of Bromine may show lower results. To avoid any measurement errors, only use glassware free of Chlorine demand. Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for one hour, then rinse all glassware thoroughly with deionised water.

  • Page 52: Bromine

    1.1 Methods Bromine with Tablet 0.05 – 1 mg/l Br 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.
  • Page 53 1.1 Methods Notes: 1. Vial cleaning: As many household cleaners (e.g. dishwater detergent) contain reducing substances, the subsequent determination of Bromine may show lower results. To avoid any measurement errors, only use glassware free of Chlorine demand. Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for one hour, then rinse all glassware thoroughly with deionised water.

  • Page 54: Bromine

    1.1 Methods Bromine with Tablet 0.05 – 6.5 mg/l Br 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 55 1.1 Methods Notes: 1. Vial cleaning: As many household cleaners (e.g. dishwater detergent) contain reducing substances, the subsequent determination of Bromine may show lower results. To avoid any measurement errors, only use glassware free of Chlorine demand. Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for one hour, then rinse all glassware thoroughly with deionised water.

  • Page 56: Cadmium

    1.1 Methods Cadmium with MERCK Spectroquant Cell Test, ® Nr. 1.14834.0001 0,025 – 0,75 mg/l Cd / 25 – 750 μg/l Cd Use two clean Reagent tubes and mark one as blank for zeroing. 1. Add 5 ml deionised water in the vial marked as blank (this is the blank, note 6).
  • Page 57 1.1 Methods Zero accepted 12. Press TEST key. prepare Test press TEST Wait for a reaction period of 2 minutes. Countdown After the reaction period is finished the measurement 2:00 starts automatically. The result is shown in the display as Cadmium. Notes: 1.

  • Page 58: Chloride With Tablet

    1.1 Methods Chloride with Tablet – 0.5 – 25 mg/l Cl 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 59 1.1 Methods Notes: 1. Ensure that all particles of the tablet are dissolved – Chloride causes an extremely fine distributed turbidity with a milky appearance. Heavy shaking leads to bigger sized particles which can cause false readings. 2. High concentrations of electrolytes and organic compounds have different effects on the precipitation reaction.

  • Page 60: Chloride

    1.1 Methods Chloride with Reagent Test – 5 – 60 mg/l Cl Use two clean vials (24 mm Ø) and mark one as blank for zeroing. 1. Fill a clean vial (24 mm Ø) with 10 ml of deionised water (this is the blank). 2.
  • Page 61 1.1 Methods Notes: 1. The test sample and the reagents should have room temperature for test performance. 2. The test sample should have a pH of between 3 and 9. 3. Store the reagent bottles in a cool, dry place ideally at between 4°C and 8°C. 4.

  • Page 62: Chlorine

    1.1 Methods Chlorine with Tablet 0.1 – 6 mg/l Cl Chlorine with Tablet 0.02 – 0.5 mg/l Cl Chlorine with Tablet 0.02 – 3 mg/l Cl Chlorine with Tablet 0.1 – 10 mg/l Cl Chlorine with Liquid Reagent 0.02 – 3 mg/l Cl Chlorine with Powder Pack 0.01 –...
  • Page 63 1.1 Methods Notes: 1. Vial cleaning: As many household cleaners (e.g. dishwasher detergent) contain reducing substances, the subsequent determination of Chlorine may show lower results. To avoid any measurement errors, only use glassware free of Chlorine demand. Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for one hour, then rinse all glassware thoroughly with deionised water.

  • Page 64: Chlorine With Tablet 10 Mm

    1.1 Methods Chlorine, differentiated determination with Tablet 0.1 – 6 mg/l Cl 1. Fill a clean 10 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. 3. Press ZERO key. prepare Zero press ZERO 4.

  • Page 65: Free Chlorine

    1.1 Methods Chlorine, free with Tablet 0.1 – 6 mg/l Cl 1. Fill a clean 10 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.

  • Page 66: Total Chlorine

    1.1 Methods Chlorine, total with Tablet 0.1 – 6 mg/l Cl 1. Fill a clean 10 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.

  • Page 67: Chlorine With Tablet 50 Mm

    1.1 Methods Chlorine, differentiated determination with Tablet 0.02 – 0.5 mg/l Cl 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. 3. Press ZERO key. prepare Zero press ZERO 4.
  • Page 68 1.1 Methods Chlorine, free with Tablet 0.02 – 0.5 mg/l Cl 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.
  • Page 69 1.1 Methods Chlorine, total with Tablet 0.02 – 0.5 mg/l Cl 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.

  • Page 70: Chlorine With Tablet 24 Mm

    1.1 Methods Chlorine, free with Tablet 0.02 – 3 mg/l Cl 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 71 1.1 Methods Chlorine, total with Tablet 0.02 – 3 mg/l Cl 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 72 1.1 Methods Chlorine, differentiated determination with Tablet 0.02 – 3 mg/l Cl 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 73 1.1 Methods 13. Place the vial in the sample chamber making sure that marks are aligned. T1 accepted 14. Press TEST key. prepare T2 press TEST Wait for a reaction period of 2 minutes. Countdown 2:00 After the reaction period is finished the measurement starts automatically.

  • Page 74: Chlorine Hr With Tablet 10 Mm

    1.1 Methods Chlorine HR, free with Tablet 0.1 – 10 mg/l Cl 1. Fill a clean 10 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.

  • Page 75: Total Chlorine

    1.1 Methods Chlorine HR, total with Tablet 0.1 – 10 mg/l Cl 1. Fill a clean 10 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.
  • Page 76 1.1 Methods Chlorine HR, differentiated determination with Tablet 0.1 – 10 mg/l Cl 1. Fill a clean 10 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3.
  • Page 77 1.1 Methods 12. Add one DPD No. 3 HR tablet straight from the foil to the same water sample and crush the tablet using a clean stirring rod. Dissolve the tablet. 13. Fill the 10 mm cell with the coloured test solution. 14.

  • Page 78: Chlorine With Liquid Reagent 24 Mm

    1.1 Methods Chlorine, free with Liquid Reagent 0.02 – 3 mg/l Cl 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.

  • Page 79: Total Chlorine

    1.1 Methods Chlorine, total with Liquid Reagent 0.02 – 3 mg/l Cl 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.

  • Page 80: Differentiated Determination (Free, Combined, Total)

    1.1 Methods Chlorine, differentiated determination with Liquid Reagent 0.02 – 3 mg/l Cl 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 81 1.1 Methods 13. Place the vial in the sample chamber making sure that marks are aligned. 14. Press TEST key. T1 accepted prepare T2 Wait for a reaction period of 2 minutes. press TEST Countdown 2:00 After the reaction period is finished the measurement starts automatically.

  • Page 82: Chlorine (Powder Pack) 24 Mm

    1.1 Methods Chlorine, free with Powder Pack 0.01 – 2 mg/l Cl 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.

  • Page 83: Total Chlorine

    1.1 Methods Chlorine, total with Powder Pack 0.01 – 2 mg/l Cl 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.

  • Page 84: Differentiated Determination (Free, Combined, Total)

    1.1 Methods Chlorine, differentiated determination with Powder Pack 0.01 – 2 mg/l Cl 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 85 1.1 Methods 12. Place the vial in the sample chamber making sure that marks are aligned. T1 accepted 13. Press TEST key. prepare T2 press TEST Wait for a reaction period of 3 minutes. Countdown 3:00 After the reaction period is finished the measurement starts automatically.

  • Page 86: Chlorine Mr (Vario Powder Pack)

    1.1 Methods Chlorine MR, free with Vario Powder Pack 0.01 – 3.5 mg/l Cl 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.

  • Page 87: Total Chlorine

    1.1 Methods Chlorine MR, total with Vario Powder Pack 0.01 – 3.5 mg/l Cl 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.

  • Page 88: Differentiated Determination (Free, Combined, Total)

    1.1 Methods Chlorine MR, differentiated determination with Vario Powder Pack 0.01 – 3.5 mg/l Cl 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 89 1.1 Methods 12. Place the vial in the sample chamber making sure that marks are aligned. T1 accepted 13. Press TEST key. prepare T2 press TEST Wait for a reaction period of 3 minutes. Countdown 3:00 After the reaction period is finished the measurement starts automatically.
  • Page 90 1.1 Methods Chlorine HR (Kl) with Tablet 5 – 200 mg/l Cl 1. Fill a clean vial (16 mm Ø) with 8 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that Ø...

  • Page 91: Chlorine Hr (Ki)

    1.1 Methods Notes: 1. Oxidizing agents interfere as they react in the same way as Chlorine. Reagent Form of reagent/Quantity Order-No. Set ACIDIFYING GP/ Tablet / per 100 4517721BT CHLORINE HR (KI) inclusive stirring rod CHLORINE HR (KI) Tablet / 100 4513000BT ACIDIFYING GP Tablet / 100...

  • Page 92: Chlorine Dioxide In Absence Of Chlorine

    1.1 Methods Chlorine dioxide in absence of Chlorine with Tablet 0.05 – 1 mg/l ClO 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3.
  • Page 93 1.1 Methods Notes: 1. Vial cleaning: As many household cleaners (e.g. dishwasher detergent) contain reducing substances, the subsequent determination of Oxidizing agents (e.g. Chlorine, Bromine) may show lower results. To avoid any measurement errors, only use glassware free of Chlorine demand.

  • Page 94: Chlorine Dioxide

    1.1 Methods Chlorine dioxide with Tablet 0.05 – 2.5 mg/l ClO Chlorine dioxide The following selection is shown in the display: >> with Cl without Cl for the determination of Chlorine dioxide in the presence >> with Cl of Chlorine. for the determination of Chlorine dioxide in the absence >>...
  • Page 95 1.1 Methods Notes: 1. Vial cleaning: As many household cleaners (e.g. dishwasher detergent) contain reducing substances, the subsequent determination of Chlorine dioxide may show lower results. To avoid any measurement errors, only use glassware free of Chlorine demand. Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for one hour, then rinse all glassware thoroughly with deionised water.

  • Page 96: In Presence Of Chlorine

    1.1 Methods Chlorine dioxide in the presence of Chlorine with Tablet 0.05 – 2.5 mg/l ClO 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample. 2. Add one GLYCINE tablet straight from the foil and crush the tablet using a clean stirring rod.
  • Page 97 1.1 Methods 13. Remove the vial from the sample chamber, empty the vial, rinse vial and cap several times. Fill with a few drops of water sample. 14. Add one DPD No. 1 tablet straight from the foil and crush the tablet using a clean stirring rod. 15.
  • Page 98 1.1 Methods Notes: (Chlorine dioxide in the presence of Chlorine) 1. The conversion factor to convert Chlorine dioxide as Chlorine to Chlorine dioxide as ClO is approximately 0.4 (more exactly 0.38). mg/l ClO = mg/l ClO [Cl] x 0.38 [Cl] (Chlorine dioxide displayed as Chlorine units ClO [Cl] has its origin in swimming poolwater treatment according to DIN 19643.)

  • Page 99: In Absence Of Chlorine

    1.1 Methods Chlorine dioxide in absence of Chlorine with Tablet 0.05 – 2.5 mg/l ClO 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.

  • Page 100: Chromium (Powder Pack)

    1.1 Methods Chromium with Powder Pack 0.005 – 0.5 mg/l Cr / 5 – 500 μg/l Cr Chromium with Powder Pack 0.02 – 2 mg/l Cr Chrom >> diff The following selection is shown in the display: Cr (VI) Cr (III + VI) for the differentiated determination of Chromium (VI), >>...
  • Page 101 AL 800 / SpectroDirect_8 05/2017...

  • Page 102: Differentiated Determination

    1.1 Methods Chromium, differentiated determination with Powder Pack 0.005 – 0.5 mg/l Cr / 5 – 500 μg/l Cr Digestion: 1. Fill 10 ml of water sample into a clean vial (16 mm Ø). 2. Add the contents of one PERSULF.RGT FOR CR Powder Pack straight from the foil into the vial.
  • Page 103 1.1 Methods 15. Fill a second clean vial (16 mm Ø) with 10 ml of the water sample. 16. Add the contents of one CHROMIUM HEXAVALENT Powder Pack straight from the foil to the water sample. Ø 16 mm 17. Close the vial tightly with the cap and swirl several times to mix the contents.

  • Page 104: Chromium (Vi)

    1.1 Methods Chromium (Vl) with Powder Pack 0.005 – 0.5 mg/l Cr / 5 – 500 μg/l Cr 1. Fill a clean 50 mm cell with deionised water. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3.

  • Page 105: Chromium, Total (Cr(Iii) + Cr(Vi))

    1.1 Methods Chromium, total (Cr(lll) + Cr(Vl)) with Powder Pack 0.005 – 0.5 mg/l Cr / 5 – 500 μg/l Cr Digestion: 1. Fill a clean vial (16 mm Ø) with 10 ml of water sample. 2. Add the contents of one PERSULF.RGT FOR CR Powder Pack straight from the foil into the vial.

  • Page 106: Differentiated Determination

    1.1 Methods Chromium, differentiated determination with Powder Pack 0.02 – 2 mg/l Cr Digestion: 1. Fill a clean vial (16 mm Ø) with 10 ml of water sample. 2. Add the contents of one PERSULF.RGT FOR CR Powder Pack straight from the foil into the vial. Ø...
  • Page 107 1.1 Methods 13. Fill a second clean vial (16 mm Ø) with 10 ml of the water sample. 14. Add the contents of one CHROMIUM HEXAVALENT Powder Pack straight from the foil to the water sample. Ø 16 mm 15. Close the vial tightly with the cap and swirl several times to mix the contents.

  • Page 108: Chromium (Vi)

    1.1 Methods Chromium (Vl) with Powder Pack 0.02 – 2 mg/l Cr 1. Fill a clean vial (16 mm Ø) with 10 ml of the water sample. 2. Place the vial in the sample chamber making sure that Ø 16 mm the marks are aligned.

  • Page 109: Chromium, Total (Cr(Iii) + Cr(Vi))

    1.1 Methods Chromium, total (Cr(lll) + Cr(Vl)) with Powder Pack 0.2 – 2 mg/l Cr Digestion: 1. Fill a clean vial (16 mm Ø) with 10 ml of water sample. 2. Add the contents of one PERSULF.RGT FOR CR Powder Pack straight from the foil into the vial. Ø...

  • Page 110: Cod, Low Range (Lr)

    1.1 Methods COD LR with Vario Tube Test 3 – 150 mg/l O 1. Open one white capped reaction vial and add 2 ml deionised water (this is the blank (Note 1)). 2. Open another white capped reaction vial and add 2 ml of water sample (this is the sample).
  • Page 111 1.1 Methods Notes: 1. Run samples and blanks with the same batch of vials. The blank is stable when stored in the dark and can be used for further measurements with vials of the same batch. 2. Do not place the hot vials in the sample chamber. Cool the vials to room temperature for final measurements.

  • Page 112: Cod, Middle Range (Mr)

    1.1 Methods COD MR with Vario Tube Test 20 – 1500 mg/l O 1. Open one white capped reaction vial and add 2 ml deionised water (this is the blank (Note 1)). 2. Open another white capped reaction vial and add 2 ml water sample (this is the sample).
  • Page 113 1.1 Methods Notes: 1. Run samples and blanks with the same batch of vials. The blank is stable when stored in the dark and can be used for further measurements with vials of the same batch. 2. Do not place the hot vials in the sample chamber. Cool the vials to room temperature for final measurements.

  • Page 114: Cod, High Range (Hr)

    1.1 Methods COD HR with Vario Tube Test 0.2 – 15 g/l O 200 – 15 000 mg/l O 1. Open one white capped reaction vial and add 0.2 ml deionised water (this is the blank (Note 1)). 2. Open another white capped reaction vial and add 0.2 ml water sample (this is the sample).
  • Page 115 1.1 Methods Notes: 1. Run samples and blanks with the same batch of vials. The blank is stable when stored in the dark and can be used for further measurements with vials of the same batch. 2. Do not place the hot vials in the sample chamber. Cool the vials to room temperature for final measurements.

  • Page 116: Colour, True And Apparent

    1.1 Methods Colour, true and apparent (APHA Platinum-Cobalt Standard Method) 0 – 500 Pt-Co units Sample preparation (Note 4): Step A Filter approx. 50 ml deionised water through a membrane filter with a pore width of 0.45 μm. Discard the filtrate. Filter another 50 ml deionised water and keep it for zeroing.
  • Page 117 1.1 Methods Notes: 1. This colour scale was originally developed by A. Hazen as a visual comparison scale. It is therefore necessary to ascertain whether the extinction maximum of the water sample is in the range from 420 to 470 nm, as this method is only suitable for water samples with yellowish to yellowish-brown coloration.

  • Page 118: Copper With Tablet

    1.1 Methods Copper with Tablet 0.05 – 1 mg/l Cu Copper with Tablet 0.5 – 5 mg/l Cu Copper >> diff The following selection is shown in the display: free total for the differentiated determination of free, combined and >> diff total Copper.
  • Page 119 1.1 Methods Note: 1. If ??? is displayed at the diffentiated test result see page 356. Reagent Form of reagent/Quantity Order-No. Tablet / per 100 4517691BT COPPER No. 1 / No. 2 inclusive stirring rod COPPER No. 1 Tablet / 100 4513550BT COPPER No.

  • Page 120: Differentiated Determination (Free, Combined, Total)

    1.1 Methods Copper, differentiated determination with Tablet 0.05 – 1 mg/l Cu 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.
  • Page 121 1.1 Methods 12. Fill the 50 mm cell with the coloured test solution. 13. Place the cell in the sample chamber making sure that the positioning is correct. T1 accepted prepare T2 14. Press TEST key. press TEST The result is shown in the display in: *,** mg/l free Cu mg/l free Copper *,** mg/l comb Cu...

  • Page 122: Free Copper

    1.1 Methods Copper, free with Tablet 0.05 – 1 mg/l Cu 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.

  • Page 123: Total Copper

    1.1 Methods Copper, total with Tablet 0.05 – 1 mg/l Cu 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.

  • Page 124: Differentiated Determination (Free, Combined, Total)

    1.1 Methods Copper, differentiated determination with Tablet 0.5 – 5 mg/l Cu 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 125 1.1 Methods 12. Place the vial in the sample chamber making sure that marks are aligned. T1 accepted 13. Press TEST key. prepare T2 press TEST The result is shown in the display in: *,** mg/l free Cu mg/l free Copper *,** mg/l comb Cu mg/l combined Copper *,** mg/l total Cu...

  • Page 126: Free Copper

    1.1 Methods Copper, free with Tablet 0.5 – 5 mg/l Cu 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.

  • Page 127: Total Copper

    1.1 Methods Copper, total with Tablet 0.5 – 5 mg/l Cu 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.

  • Page 128: Copper, Free (Powder Pack)

    1.1 Methods Copper, free (Note 1) with Vario Powder Pack 0.05 – 5 mg/l Cu 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 129 1.1 Methods Notes: 1. For determination of total Copper digestion is required. 2. Extremely acid water samples (pH 2 or less) must be adjusted between pH 4 and pH 6 before the reagent is added (with 8 mol/l Potassium hydroxide solution KOH). Caution: pH values above 6 can lead to Copper precipitation.

  • Page 130: Cyanide

    1.1 Methods Cyanide with Reagent Test 0.005 – 0.2 mg/l CN / 5 – 200 μg/l CN 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3.
  • Page 131 1.1 Methods Notes: 1. Only free Cyanide and Cyanides that can be destroyed by Chlorine are determined by this test. 2. In the presence of Thiocyanate, heavy metal complexes, colorants or aromatic amines, the cyanide must be separated out by distillation before analysis is performed. 3.

  • Page 132: Cyanide

    1.1 Methods Cyanide with Reagent Test 0.01 – 0.5 mg/l CN 1. Fill a clean vial (24 mm Ø) with 2 ml of the water sample and 8 ml of deionised water, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 133 1.1 Methods Notes: 1. Only free Cyanide and Cyanides that can be destroyed by Chlorine are determined by this test. 2. In the presence of Thiocyanate, heavy metal complexes, colorants or aromatic amines, the cyanide must be separated out by distillation before analysis is performed. 3.

  • Page 134: Cya-Test (Cyanuric Acid)

    1.1 Methods CyA-TEST (Cyanuric acid) with Tablet 0 – 160 mg/l CyA 1. Fill a clean vial (24 mm Ø) with 5 ml of the water sample and 5 ml deionised water (Note 1), close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 135 1.1 Methods Notes: 1. Use deionised water or tap water free of Cyanuric acid. 2. If Cyanuric acid is present a cloudy solution will occur. Small single particles are not necessarily caused by Cyanuric acid. 3. Dissolve the tablet completely (therefore swirl the vial approx. 1 minute). Un-dissolved particles of the tablet can cause results that are too high.

  • Page 136: Deha T

    1.1 Methods DEHA (N,N-Diethylhydroxylamine) with Tablet and Liquid Reagent 0.02 – 0.5 mg/l / 20 – 500 μg/l DEHA 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap (Note 2). 2.
  • Page 137 1.1 Methods Notes: 1. Application: Testing of residual corrosion inhibitors (Oxygen scavengers) in boiler feed water or condensate. 2. Before using clean the vials with Hydrochloric acid (approx. 20%). Rinse thoroughly with deionised water. 3. Keep the sample dark during colour development time. UV-light (sunlight) causes high measurement results.

  • Page 138: Deha (Powder Pack)

    1.1 Methods DEHA (N,N-Diethylhydroxylamin) with Vario Powder Pack and Liquid Reagent 0.02 – 0.5 mg/l / 20 – 500 μg/l DEHA Use two clean vials (24 mm Ø) and mark one as blank for zeroing (Note 2). 1. Fill a clean vial with 10 ml deionised water (this is the blank).
  • Page 139 1.1 Methods Notes: 1. Application: Testing of residual corrosion inhibitors (Oxygen scavengers) in boiler feed water or condensate. 2. Before using clean the vials with Hydrochloric acid (approx. 20%). Rinse thoroughly with deionised water. 3. Ideally temperature for full colour development is 25°C ± 3 °C. 4.

  • Page 140: Fluoride

    1.1 Methods Fluoride 0.05 – 1.5 mg/l F Caution: See notes! 1. Fill a clean vial (24 mm Ø, Note 8) with exactly 10 ml of water sample (Note 4), close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 141 1.1 Methods Notes: 1. The same batch of SPADNS reagent solution must be used for adjustment and test. The adjustment process needs to be performed for each new batch of SPADNS reagent solution (see Standard Methods 20th, 1998, APHA, AWWA, WEF 4500 F D., S. 4-82). The procedure is described in chapter 2.4.5 “Calibration –...

  • Page 142: Formaldehyde, 10 Mm

    1.1 Methods Formaldehyde with MERCK Spectroquant Test, ® No. 1.14678.0001 1 – 5 mg/l HCHO Use two clean, empty vials and mark one as blank for zeroing. 1. Pipette 4.5 ml reagent HCHO-1 into each vial. (CAUTION: Reagent contains concentrated Sulfuric acid! Note 4) 2.
  • Page 143 1.1 Methods Notes: 1. This method is adapted from MERCK. 2. Before performing the test read the original test instructions (delivered with the test) and the MSDS (available at www.merckmillipore.com). 3. Spectroquant is a registered trade mark of the company MERCK KGaA. ®...

  • Page 144: Formaldehyde, 50 Mm

    1.1 Methods Formaldehyde with MERCK Spectroquant Test, ® No. 1.14678.0001 0.02 – 1 mg/l HCHO Use two clean, empty vials and mark one as blank for zeroing. 1. Pipette 4.5 ml reagent HCHO-1 into each vial. (CAUTION: Reagent contains concentrated Sulfuric acid! Note 4) 2.
  • Page 145 1.1 Methods Notes: 1. This method is adapted from MERCK. 2. Before performing the test read the original test instructions (delivered with the test) and the MSDS (available at www.merckmillipore.com). 3. Spectroquant is a registered trade mark of the company MERCK KGaA. ®...

  • Page 146: Formaldehyde, 16 Mm

    1.1 Methods Formaldehyde with MERCK Spectroquant Cell Test, ® No. 1.14500.0001 0,1 – 5 mg/l HCHO Use two clean Reagent tubes and mark one as blank for zeroing. 1. Add one level microspoon of HCHO-1K into each Ø 16 mm vial.
  • Page 147 1.1 Methods 10. Place the vial (the sample) in the sample chamber making sure that the marks are aligned. Zero akzeptiert Test vorbereiten 11. Press TEST key. TEST drücken The result is shown in the display in mg/l Formaldehyde. Notes: 1.

  • Page 148: Hardness, Total

    1.1 Methods Hardness, total with Tablet 2 – 50 mg/l CaCO 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 149 1.1 Methods Notes: 1. Strong alkaline or acidic water samples must be adjusted between pH 4 and pH 10 before the tablet is added (use 1 mol/l Hydrochloric acid resp. 1 mol/l Sodium hydroxide). 2. Conversion table: mg/l CaCO °dH °fH °eH 1 mg/l CaCO...

  • Page 150: Hardness, Total Hr

    1.1 Methods Hardness, total HR with Tablet 20 – 500 mg/l CaCO 1. Fill a clean vial (24 mm Ø) with 1 ml of the water sample and 9 ml of deionised water, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 151 1.1 Methods Notes: 1. Strong alkaline or acidic water samples must be adjusted between pH 4 and pH 10 before the tablet is added (use 1 mol/l Hydrochloric acid resp. 1 mol/l Sodium hydroxide). 2. Conversion table: mg/l CaCO °dH °fH °eH 1 mg/l CaCO...

  • Page 152: Hydrazine

    1.1 Methods Hydrazine with Powder Reagent 0.05 – 0.5 mg/l N / 50 – 500 μg/l N 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample (Note 1, 2), close tightly with the cap. 2.
  • Page 153 1.1 Methods Notes: 1. If the water sample is cloudy, you must filter it before performing the zero calibration. 2. The temperature of the water sample should not exceed 21°C. 3. Using the Hydrazine spoon: 1 g is equivalent to one level spoon. 4.

  • Page 154: Hydrazine With Liquid Reagent

    1.1 Methods Hydrazine with Vario Liquid Reagent 0.005 – 0.6 mg/l N / 5 – 600 μg/l N Use two clean vials (24 mm Ø) and mark one as blank for zeroing. 1. Fill a clean vial with 10 ml deionised water (this is the blank).
  • Page 155 1.1 Methods Notes: 1. Samples cannot be preserved and must be analysed immediately. 2. Sample temperature should be 21°C ± 4 °C. 3. The blank may develop a faint yellow colour due to the reagent. 4. Interferences: • Ammonia causes no interferences up to 10 mg/l. At a concentration of 20 mg/l it is possible that the test result increases by 20 %.

  • Page 156: Hydrogen Peroxide

    1.1 Methods Hydrogen peroxide with Tablet 0.01 – 0.5 mg/l H 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.
  • Page 157 1.1 Methods Notes: 1. Vial cleaning: As many household cleaners (e.g. dishwater detergent) contain reducing substances, the subsequent determination of Hydrogen peroxide may show lower results. To avoid any measurement errors, only use glassware free of Chlorine demand. Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for one hour, then rinse all glassware thoroughly with deionised water.

  • Page 158: Hydrogen Peroxide

    1.1 Methods Hydrogen peroxide with Tablet 0.03 – 1.5 mg/l H 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 159 1.1 Methods Notes: 1. Vial cleaning: As many household cleaners (e.g. dishwasher detergent) contain reducing substances, the subsequent determination of Hydrogen peroxide may show lower results. To avoid any measurement errors, only use glassware free of Chlorine demand. Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for one hour, then rinse all glassware thoroughly with deionised water.

  • Page 160: Iodine

    1.1 Methods Iodine with Tablet 0.05 – 3.6 mg/l I 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 161 1.1 Methods Notes: 1. Oxidising reagents, such as Chlorine, Bromine, etc. interfere as they react in the same way as Iodine. Reagent Form of reagent/Quantity Order-No. DPD No. 1 Tablet / 100 4511050BT AL 800 / SpectroDirect_8 05/2017...

  • Page 162: Iron

    1.1 Methods Iron with Tablet 0.1 – 1 mg/l Fe * Determination of total dissolved Iron Fe and Fe Iron with Tablet 0.01 – 0.5 mg/l Fe * Determination of total dissolved Iron Fe and Fe Iron with Tablet 0.1 – 1 mg/l Fe * Determination of total dissolved Iron Fe and Fe Iron...
  • Page 163 1.1 Methods Notes: Iron Determination dissolved and dissolved Iron undissolved Iron Filtration Digestion + Fe + Fe IRON LR IRON (II) LR methods tablet tablet 220, 222, 223 result A minus result B = Fe Digestion procedure for the determination of total dissolved and undissolved iron. 1.

  • Page 164: Iron With Tablet

    1.1 Methods Iron with Tablet 0.1 – 1 mg/l Fe 1. Fill a clean 10 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.
  • Page 165 1.1 Methods Notes: 1. This method determines the total dissolved Iron as Fe and Fe 2. For the determination of Fe ions the IRON (II) LR tablet is used, as described above, instead of the IRON LR tablet. 3. For the determination of total dissolved and undissolved iron digestion is required. An example is described on page 155.

  • Page 166: Iron With Tablet

    1.1 Methods Iron with Tablet 0.01 – 0.5 mg/l Fe 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.
  • Page 167 1.1 Methods Notes: 1. This method determines the total dissolved Iron as Fe and Fe 2. For the determination of Fe ions the IRON (II) LR tablet is used, as described above, instead of the IRON LR tablet. 3. For the determination of total dissolved and undissolved iron digestion is required. An example is described on page 155.

  • Page 168: Iron With Tablet

    1.1 Methods Iron (Note 1) with Tablet 0.1 – 1 mg/l Fe 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 169 1.1 Methods Notes: 1. This method determines the total dissolved Iron as Fe and Fe 2. For the determination of Fe ions the IRON (II) LR tablet is used, as described above, instead of the IRON LR tablet. 3. For the determination of total dissolved and undissolved iron digestion is required. An example is described on page 155.

  • Page 170: Iron (Powder Pack)

    1.1 Methods Iron (Note 1) with Vario Powder Pack 0.1 – 3 mg/l Fe 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that the marks are aligned.
  • Page 171 1.1 Methods Notes: 1. The reagent reacts with all dissolved iron and most undissolved forms of iron in the water sample. 2. Iron oxide requires prior digestion: use mild, vigorous or Digesdahl digestion (e.g. for digestion with acid see page 155). 3.

  • Page 172: Iron Tptz (Powder Pack)

    1.1 Methods Iron, total (TPTZ, Note 1) with Vario Powder Pack 0.1 – 1.8 mg/l Fe Use two clean vials (24 mm Ø) and mark one as blank for zeroing. 1. Fill a clean vial with 10 ml deionised water (this is the blank).
  • Page 173 1.1 Methods Notes: 1. For determination of total Iron digestion is required. TPTZ reagent recovers most insoluble iron oxides without digestion. 2. Rinse all glassware with 1:1 Hydrochloric acid solution first and then rinse with deionised water to remove iron deposits that can cause slightly high results. 3.

  • Page 174: Lead, 10 Mm

    1.1 Methods Lead with MERCK Spectroquant Test, ® No. 1.09717.0001 0.1 – 5 mg/l Pb 1. Fill a clean 10 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3.
  • Page 175 1.1 Methods Notes: 1. This method is adapted from MERCK. 2. Before performing the test read the original test instructions (delivered with the test) and the MSDS (available at www.merckmillipore.com). 3. Spectroquant is a registered trade mark of the company MERCK KGaA. ®...

  • Page 176: Lead, 16 Mm

    1.1 Methods Lead with MERCK Spectroquant Cell Test, ® No. 1.14833.0001 0.1 – 5 mg/l Pb Procedure A Select this test for the determination of lead in soft to medium hard waters with a Ca contents lower than 70 mg/l (approx. 10°d). Procedure B Select this test for the determination of lead in hard to very hard waters with a Ca...

  • Page 177: Procedure A

    1.1 Methods Lead with MERCK Spectroquant Cell Test, ® No. 1.14833.0001 0.1 – 5 mg/l Pb Procedure A Use two clean Reagent tubes and mark one as blank for zeroing. Caution! Reagent tubes contain Potassium Cyanide! Ø 16 mm Adhere strictly to the specified dosage sequence! (Note 4) 1.
  • Page 178 1.1 Methods Lead with MERCK Spectroquant Cell Test, ® No. 1.14833.0001 0,1 – 5 mg/l Pb Procedure A Use two clean Reagent tubes and mark one as blank for zeroing. Ø 16 mm Caution! Reagent tubes contain Potassium Cyanide! Adhere strictly to the specified dosage sequence! (Note 4) 1.
  • Page 179 1.1 Methods 9. Place the vial (the sample) in the sample chamber making sure that the marks are aligned. Zero accepted prepare T1 10. Press TEST key. press TEST 11. Remove the vial (the sample) from the sample cham- ber and open carefully. 12.

  • Page 180: Manganese With Tablet

    1.1 Methods Manganese with Tablet 0.2 – 4 mg/l Mn 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 181 1.1 Methods Note: KMnO Reagent Form of reagent/Quantity Order-No. Tablet / per 100 4517621BT MANGANESE LR No. 1 / No. 2 inclusive stirring rod MANGANESE LR No. 1 Tablet / 100 4516080BT MANGANESE LR No. 2 Tablet / 100 4516090BT AL 800 / SpectroDirect_8 05/2017...

  • Page 182: Manganese Lr (Powder Pack)

    1.1 Methods Manganese LR with Vario Powder Pack 0.01 – 0.7 mg/l Mn Use two clean vials (24 mm Ø) and mark one as blank for zeroing (Note 1). 1. Fill a clean vial with 10 ml of deionised water (this is the blank).
  • Page 183 1.1 Methods Notes: 1. Rinse all glassware with 1:1 Nitric acid solution first and then rinse with deionised water. 2. Water samples that contain more than 300 mg/l CaCO hardness: after adding the Vario Ascorbic Acid powder pack add additionally 10 drops of Rochelle Salt Solution. 3.

  • Page 184: Manganese Hr (Powder Pack)

    1.1 Methods Manganese HR with Vario Powder Pack 0.1 – 18 mg/l Mn 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 185 1.1 Methods Notes: 1. This test is applicable for the determination of soluble Manganese in water and wastewater. 2. Highly buffered water samples or extreme pH values may exceed the buffering capacity of the reagents and requires sample pre-treatment. If samples were acidified for storing, adjust the pH between 4 and 5 with 5 mol/l (5 N) Sodium hydroxide before test.

  • Page 186: Molybdate With Tablet

    1.1 Methods Molybdate / Molybdenum with Tablet 1 – 30 mg/l MoO / 0.6 – 18 mg/l Mo 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 187 1.1 Methods Notes: 1. The tablets must be added in the correct sequence. 2. Under test conditions (pH 3.8 – 3.9) iron does not interfere nor do other metals at levels likely to be found in industrial water systems. 3. Conversions: mg/l Mo = mg/l MoO x 0.6 mg/l Na...

  • Page 188: Molybdate Lr (Powder Pack)

    1.1 Methods Molybdate / Molybdenum LR mit Vario Powder Pack 0.05 – 5.0 mg/l MoO / 0.03 – 3 mg/l Mo 1. Fill a clean Mixing Cylinder (25 ml) with 20 ml of the water sample. 2. Add the contents of one Vario Molybdenum 1 LR F20 Powder Pack straight from the foil into the water sample (20 ml).
  • Page 189 1.1 Methods prepare Zero 12. Press ZERO key. press ZERO 13. Remove the vial from the sample chamber. 14. Place the sample in the sample chamber making sure that the marks are aligned. Zero accepted prepare Test 15. Press TEST key. press TEST The result is shown in the display in mg/l Molybdate / Molybdenum.

  • Page 190: Molybdate Hr (Powder Pack)

    1.1 Methods Molybdate / Molybdenum HR with Vario Powder Pack 0.5 – 66 mg/l MoO / 0.3 – 40 mg/l Mo 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2.
  • Page 191 1.1 Methods Notes: 1. Filter turbid water samples using filter paper and funnel before analysis. 2. Highly buffered water samples or extreme pH values should be adjusted to a pH of nearly 7 with 1 mol/l Nitric acid or 1 mol/l Sodium hydroxide. 3.

  • Page 192: Nickel

    1.1 Methods Nickel with Reagent Test 0.02 – 1 mg/l Ni 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.
  • Page 193 1.1 Methods Notes: 1. The test sample and the reagents should have room temperature for test performance. 2. The test sample should have a pH of between 3 and 9. Reagent / Accessories Form of reagent/Quantity Order-No. SET: Reagent Test / approx. 50 Tests 419033 NICKEL 51 / 52 AL 800 / SpectroDirect_8 05/2017...

  • Page 194: Nickel

    1.1 Methods Nickel with Reagent Test 0.2 – 7 mg/l Ni 1. Fill a clean vial (24 mm Ø) with 3 ml of the water sample and 7 ml of deionised water, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 195 1.1 Methods Notes: 1. The test sample and the reagents should have room temperature for test performance. 2. The test sample should have a pH of between 3 and 9. Reagent / Accessories Form of reagent/Quantity Order-No. SET: Reagent Test / approx. 50 Tests 2419033 NICKEL 51 / 52 AL 800 / SpectroDirect_8 05/2017...

  • Page 196: Nitrate

    1.1 Methods Nitrate with Tube Test 1 – 30 mg/l N 1. Open one white capped reaction vial (Reagent A) and add 1 ml deionised water (this is the blank). 2. Open another white capped reaction vial (Reagent A) Ø 16 mm and add 1 ml water sample (this is the sample).
  • Page 197 1.1 Methods Notes: 1. Some solids may not dissolve. 2. Conversion: mg/l NO = mg/l N x 4.43 Reagent Form of reagent/Quantity Order-No. 4535580 VARIO Nitrate Chromotropic Powder Pack / 50 VARIO Nitra X Reagent tube Reaction tube / 50 VARIO deionised water 100 ml AL 800 / SpectroDirect_8 05/2017...

  • Page 198: Nitrate Lr

    1.1 Methods Nitrate LR with Tube Test 0.5 – 14 mg/l N 1. Place the supplied blank (red label) in the sample chamber making sure that the marks are aligned. Ø 16 mm 2. Press ZERO key. prepare Zero 3. Remove the vial from the sample chamber. press ZERO 4.
  • Page 199 1.1 Methods Notes: – 1. Nitrite concentrations greater than 2 mg/L NO lead to higher test results. 2. Great quantities of COD lead to higher test results. Reagent / Accessories Form of reagent/Quantity Order-No. Set: Tube Test (Liquid reagent) / 24 Tests 420702 Reaktion tube NITRATE-111...

  • Page 200: Nitrite With Tablet

    1.1 Methods Nitrite with Tablet 0.01 – 0.5 mg/l N 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that the marks are aligned.
  • Page 201 1.1 Methods Notes: 1. The following ions can produce interferences since under the reaction conditions they cause precipitation: Antimony (III), Iron (III), Lead, Mercury (I), Silver, Chloroplatinate, Metavanadate and Bismuth. Copper (II)-ions may cause lower test results as they accelerate the decomposition of the Diazonium salt.

  • Page 202: Nitrite Lr (Powder Pack)

    1.1 Methods Nitrite LR with Vario Powder Pack 0.01 – 0.3 mg/l N 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 203 1.1 Methods Notes: 1. Interferences: • Strong oxidizing and reducing substances interfere. • Cupric and ferrous ions cause low results. • Antimonous, Auric, Bismuth, Chloroplatinate, Ferric, Lead, Mercurous, Metavanadate, Silver ions interfere by causing precipitation. • In samples with very high concentrations of Nitrate (> 100 mg/l N) a small amount of Nitrite will be found.

  • Page 204: Nitrite Lr

    1.1 Methods Nitrite, LR with Tube Test 0.03 – 0.6 mg/l N 1. Place the supplied blank (red label) in the sample cham- ber making sure that the marks are aligned. 2. Press ZERO key. Ø 16 mm prepare Zero 3.
  • Page 205 1.1 Methods Notes: 1. Store the reagents in closed containers at a temperature of + 4°C to + 8°C. 2. The test sample and the reagents should have room temperature for test performance. Reagent / Accessories Form of reagent/Quantity Order-No. Set: Tube Test (Powder) / 24 Tests 419018...

  • Page 206: Nitrite Hr

    1.1 Methods Nitrite, HR with Tube Test 0.3 – 3 mg/l N 1. Place the supplied blank (red label) in the sample cham- ber making sure that the marks are aligned. 2. Press ZERO key. Ø 16 mm prepare Zero 3.
  • Page 207 1.1 Methods Notes: 1. Store the reagents in closed containers at a temperature of + 4°C to + 8°C. 2. The test sample and the reagents should have room temperature for test performance. Reagent / Accessories Form of reagent/Quantity Order-No. Set: Tube Test (Powder) / 24 Tests 419018...

  • Page 208: Nitrogen, Total Lr

    1.1 Methods Nitrogen, total LR with Vario Tube Test 0.5 – 25 mg/l N 1. Open two TN Hydroxide LR digestion vials and add the contents of one Vario TN Persulfate Rgt. Powder Pack (Note 2, 3). Ø 16 mm 2.
  • Page 209 1.1 Methods 11. Close the vials with the caps and shake to mix the contents (at least 15 seconds, Note 8). Countdown 2:00 12. Press [ ] key. start: Wait for a reaction period of 2 minutes. After the reaction period is finished proceed as follows: 13.

  • Page 210: Nitrogen, Total Hr

    1.1 Methods Nitrogen, total HR with Vario Tube Test 5 – 150 mg/l N 1. Open two TN Hydroxide HR digestion vials and add the contents of one Vario TN Persulfate Rgt. Powder Pack (Note 2, 3). Ø 16 mm 2.
  • Page 211 1.1 Methods 11. Close the vials with the caps and shake to mix the contents (at least 15 seconds, Note 8). Countdown 2:00 12. Press [ ] key. start: Wait for a reaction period of 2 minutes. After the reaction period is finished proceed as follows: 13.
  • Page 212 1.1 Methods Notes: 1. Appropriate safety precautions and a good lab technique should be used during the whole procedure. 2. Use a funnel to add the reagent. 3. Wipe off any Persulfate reagent that may get on the lid or the tube threads. 4.
  • Page 213 1.1 Methods Nitrogen, total LR with Vario Tube Test Reagent Form of reagent/Quantity Order-No. Tube test contains: 4535550 VARIO TN HYDROX LR Tube Digestion tube / 50 VARIO PERSULFATE Reagent Powder Pack / 50 VARIO TN Reagent A Powder Pack / 50 VARIO TN Reagent B Powder Pack / 50 VARIO TN ACID LR/HR Tube...

  • Page 214: Nitrogen, Total Lr 2

    1.1 Methods Nitrogen, total LR 2 with Tube Test 0.5 – 14 mg/l N Digestion: 1. Fill one of the supplied digestion vials with 5 ml of the water sample. 2. Add 1 level scoop No. 8 (black) digestion reagent. Ø...
  • Page 215 1.1 Methods 14. Add 0.2 ml Nitrate-111. 15. Close the vial tightly with the cap and invert several times to mix the contents. 16. Place the vial in the sample chamber making sure that the marks are aligned. 17. Press TEST key. Zero accepted prepare Test Wait for a reaction period of 15 minutes.

  • Page 216: Nitrogen, Total Hr 2

    1.1 Methods Nitrogen, total HR 2 with Tube Test 5 – 140 mg/l N Digestion: 1. Fill one of the supplied digestion vials with 0.5 ml of the water sample and 4.5 ml deionised water. 2. Add 1 level scoop No. 8 (black) digestion reagent. Ø...
  • Page 217 1.1 Methods 14. Add 0.2 ml Nitrate-111. 15. Close the vial tightly with the cap and invert several times to mix the contents. 16. Place the vial in the sample chamber making sure that the marks are aligned. 17. Press TEST key. Zero accepted prepare Test Wait for a reaction period of 15 minutes.

  • Page 218: Oxygen, Active

    1.1 Methods Oxygen, active * with Tablet 0.1 – 10 mg/l O 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 219 1.1 Methods Notes: * Active Oxygen is a synonym for a common disinfectant (based on “Oxygen”) in Swimming Pool Treatment. 1. When preparing the sample, the lost of Oxygen gases, e.g. by pipetting or shaking, must be avoided. 2. The analysis must take place immediately after taking the sample. Reagent Form of reagent/Quantity Order-No.

  • Page 220: Ozone

    1.1 Methods Ozone with Tablet 0.02 – 0.5 mg/l O Ozone with Tablet 0.02 – 1 mg/l O Ozon The following selection is shown in the display: >> with Cl without Cl for the determination of Ozone in the presence of Chlorine. >>...
  • Page 221 1.1 Methods Notes: 1. Vial cleaning: As many household cleaners (e.g. dishwasher detergent) contain reducing substances, the subsequent determination of Ozone may show lower results. To avoid any measurement errors, only use glassware free of Chlorine demand. Preparation: Put all applicable glassware into Sodium hypochlorite solution (0.1 g/l) for one hour, then rinse all glassware thoroughly with deionised water.

  • Page 222: In Presence Of Chlorine

    1.1 Methods Ozone, in the presence of Chlorine with Tablet 0.02 – 0.5 mg/l O 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3.
  • Page 223 1.1 Methods 15. Add one DPD No. 1 tablet and one DPD No. 3 tablet straight from the foil and crush the tablet using a clean stirring rod. 16. Transfer the contents of the first beaker (Glycine solution) into the prepared beaker (point 15). Dis- solve the tablets.

  • Page 224: In Absence Of Chlorine

    1.1 Methods Ozone, in absence of Chlorine with Tablet 0.02 – 0.5 mg/l O 1. Fill a clean 50 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3.
  • Page 225 1.1 Methods Notes: See page 213 Reagent Form of reagent/Quantity Order-No. Set DPD No. 1/No. 3 Tablet / per 100 4517711BT inclusive stirring rod DPD No. 1 Tablet / 100 4511050BT DPD No. 3 Tablet / 100 4511080BT GLYCINE Tablet / 100 4512170BT AL 800 / SpectroDirect_8 05/2017...

  • Page 226: In Presence Of Chlorine

    1.1 Methods Ozone, in the presence of Chlorine with Tablet 0.02 – 1 mg/l O 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 227 1.1 Methods 13. Close the vial tightly with the cap and swirl several times until the tablet is dissolved. 14. Add one DPD No. 1 tablet and one DPD No. 3 tablet straight from the foil into the first cleaned vial and crush the tablets using a clean stirring rod.

  • Page 228: In Absence Of Chlorine

    1.1 Methods Ozone, in absence of Chlorine with Tablet 0.02 – 1 mg/l O 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 229 1.1 Methods Notes: See page 213 Reagent Form of reagent/Quantity Order-No. Set DPD No. 1/No. 3 Tablet / per 100 4517711BT inclusive stirring rod DPD No. 1 Tablet / 100 4511050BT DPD No. 3 Tablet / 100 4511080BT GLYCINE Tablet / 100 4512170BT AL 800 / SpectroDirect_8 05/2017...

  • Page 230: Ph Value With Tablet

    1.1 Methods pH value 6.5 – 8.4 with Tablet 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 231 1.1 Methods Notes: 1. For photometric determination of pH-values only use PHENOL RED tablets in black printed foil pack and marked with PHOTOMETER. 2. Water samples with low values of Alkalinity-m (below 35 mg/l CaCO ) may give wrong pH readings. 3.

  • Page 232: Ph Value With Liquid Reagent

    1.1 Methods pH value 6.5 – 8.4 with Liquid Reagent 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 233 1.1 Methods Notes: 1. When testing chlorinated water the residual chlorine contents can influence the colour reaction of the liquid reagent. This can be avoided (without interfering with the pH measurement) by adding a small crystal of Sodiumthiosulfate (Na · 5 H O) to the sample before adding the PHENOL RED solution.

  • Page 234: Phenol

    1.1 Methods Phenol with Tablet 0.1 – 5 mg/l C 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 235 1.1 Methods Notes: 1. This method determines ortho- and meta-substituted phenols but not para-substituted phenols (see: “Standard Methods for Examination of Water and Wastewater, 20 Edition, 5-40 f.”). Because water samples can contain different types of phenolic compounds, the result is displayed as the equivalent concentration of Phenol (C OH).

  • Page 236: Phosphate

    1.1 Methods Phosphate, total with Vario Tube Test, 0.02 – 1.1 mg/l P Determination of ortho-Phosphate ions + condensed inorganic Phosphates + organically combined Phosphates Phosphate, total LR with Tube Test, 0.07 – 3 mg/l P Determination of ortho-Phosphate ions + condensed inorganic Phosphates + organically combined Phosphates Phosphate, total HR with Tube Test, 1.5 –...
  • Page 237 1.1 Methods General: Ortho-Phosphate ions react with the reagent to form an intense blue colour (methods 317, 318, 320, 323, 324, 325, 326). Phosphate in organic and condensed inorganic forms (meta-, pyro- and polyphosphates) must be converted to ortho-Phosphate ions before analysis. Pretreatment of the sample with acid and heat provides the conditions for hydrolysis of the condensed inorganic forms.

  • Page 238: Phosphate, Total (Tube Test)

    1.1 Methods Phosphate, total with Vario Tube Test 0.02 – 1.1 mg/l P 1. Open one white capped digestion tube PO4-P Acid reagent and add 5 ml of water sample. 2. Add the contents of one Vario Potassium Persul- fate F10 Powder Pack straight from the foil to the Ø...
  • Page 239 1.1 Methods Notes: 1. Appropriate safety precautions and a good lab technique should be used during the whole procedure. 2. Use a funnel to add the reagent. 3. The reagent does not dissolve completely. 4. see also page 229 5. Conversions: mg/l PO = mg/l P x 3.07 mg/l P...

  • Page 240: Phosphate, Total Lr (Tube Test)

    1.1 Methods Phosphat, total LR with Tube Test 0.07 – 3 mg/l P Digestion: 1. Add 5 ml of water sample into one reaction tube. 2. Add one level scoop No. 4 (white) Phosphat-103. (Close reagent bottle immediately!) Ø 16 mm 3.
  • Page 241 1.1 Methods Notes: 1. If the analysis is performed without digestion only ortho-Phosphate ions are determined. 2. See also page 229. Reagent / Accessories Form of reagent/Quantity Order-No. SET: Tube test (powder, liquid reagent) / 419019 Reaction tube 24 Tests Phosphate-103 Phosphate-101 Phosphate-102...

  • Page 242: Phosphate, Total Hr (Tube Test)

    1.1 Methods Phosphate, total HR with Tube Test 1.5 – 20 mg/l P Digestion: 1. Add 1 ml of water sample into one reaction tube. 2. Add one level scoop No. 4 (white) Phosphat-103. (Close reagent bottle immediately!) Ø 16 mm 3.
  • Page 243 1.1 Methods Notes: 1. If the analysis is performed without digestion only ortho-Phosphate ions are determined. 2. See also page 229. Reagent / Accessories Form of reagent/Quantity Order-No. SET: Tube test (powder, liquid reagent) / 420700 Reaction tube 24 Tests Phosphate-103 Phosphate-101 Phosphate-102...

  • Page 244: Phosphate, Ortho Lr With Tablet

    1.1 Methods Phosphate, ortho LR with Tablet 0.05 – 4 mg/l PO 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that the marks are aligned.
  • Page 245 1.1 Methods Notes: 1. Only ortho-Phosphate ions react. 2. The tablets must be added in the correct sequence. 3. The test sample should have a pH-Value between 6 and 7. 4. Interferences: Higher concentrations of Cu, Ni, Cr (III), V (V) and W (VI) interfere due to their colour. Silicates do not interfere (masked by Citric acid in the tablets).

  • Page 246: Phosphate, Ortho Hr With Tablet

    1.1 Methods Phosphate HR, ortho with Tablet 1 – 80 mg/l PO (Note 2) 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 247 1.1 Methods Notes: 1. For samples under 5 mg/l PO it is reccommended to analyse the water sample with method 320 ”Posphate LR, ortho with Tablet“. 2. Only ortho-Phosphate ions react. 3. see also page 229 4. Conversions: mg/l P = mg/l PO x 0.33 mg/l P = mg/l PO...

  • Page 248: Phosphate, Ortho (Powder Pack)

    1.1 Methods Phosphate, ortho with Vario Powder Pack 0.06 – 2.5 mg/l PO 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 249 1.1 Methods Notes: 1. The reagent does not dissolve completely. 2. see also page 229 3. Conversions: mg/l P = mg/l PO x 0.33 mg/l P = mg/l PO x 0.75 Reagent Form of reagent/Quantity Order-No. Powder Pack / 4531550 VARIO PHOS3 F10 2 x 50 VARIO PHOSPHATE RGT.

  • Page 250: Phosphate, Ortho (Vario Tube Test)

    1.1 Methods Phosphate, ortho with Vario Tube Test 0.06 – 5 mg/l PO 1. Open one white capped tube PO -P Dilution and add 5 ml of water sample. 2. Close the vials tightly with the caps and swirl several times to dissolve.
  • Page 251 1.1 Methods Notes: 1. Use a funnel to add the reagent. 2. The reagent does not dissolve completely. 3. see also page 229 4. Conversions: mg/l P = mg/l PO x 0.33 mg/l P = mg/l PO x 0.75 Reagent Form of reagent/Quantity Order-No.

  • Page 252: Phosphate, Ortho (Tube Test)

    1.1 Methods Phosphate, ortho (reactive) with Tube Test 3 – 60 mg/l PO 1. Place the supplied blank (red label) in the sample cham- ber making sure that the marks are aligned. 2. Press ZERO key. Ø 16 mm prepare Zero 3.
  • Page 253 1.1 Methods Notes: 1. Only ortho-Phosphate ions react. 2. See also page 229. Reagent / Accessories Form of reagent/Quantity Order-No. Reaction tube Tube test / 24 420701 AL 800 / SpectroDirect_8 05/2017...

  • Page 254: Phosphate, Hydrolyzable (Tube Test)

    1.1 Methods Phosphate, acid hydrolyzable with Vario Tube Test 0.02 – 1.6 mg/l P 1. Open one white capped digestion tube PO4-P Acid reagent and add 5 ml of water sample. 2. Close the vial tightly with the cap and invert gently several times to mix the contents.
  • Page 255 1.1 Methods Notes: 1. Appropriate safety precautions and a good lab technique should be used during the whole procedure. 2. Use a funnel to add the reagent. 3. The reagent does not dissolve completely. 4. see also page 229 5. Conversions: mg/l PO = mg/l P x 3.07 mg/l P...

  • Page 256: Phosphonates

    1.1 Methods Phosphonates Persulfate UV oxidation method with Vario Powder Pack 0 – 125 mg/l (see Table 1) 1. Choose the appropriate sample volume from table 1 (see following pages). 2. Pipette the chosen sample volume into a clean 50 ml graduated cylinder.
  • Page 257 1.1 Methods 13. Place the vial (the blank) in the sample chamber making sure that the marks are aligned. prepare Zero 14. Press ZERO key. press ZERO Wait for a reaction period of 2 minutes (Note 7). Countdown 2:00 After the reaction period is finished the measurement starts automatically.
  • Page 258 1.1 Methods Table 1: Expected range Sample volume Factor (mg/L Phosphonate) in ml 0 – 2.5 0 – 5.0 0 – 12.5 0 – 25 0 – 125 Table 2: Phosphonate type Conversion factor for active phosphonate PBTC 2.840 1.050 HEDPA 1.085 EDTMPA...
  • Page 259 1.1 Methods Interference levels decrease with increasing sample volume. Example: Iron interferes above 200 mg/L if a sample volume of 5 ml is used. At a sample volume of 10 ml the interference level decreases to 100 mg/L. Table 3: Interfering substances Interference level using 5 ml of sample Aluminium...

  • Page 260: Potassium

    1.1 Methods Potassium with Tablet 1 – 10 mg/l K 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 261 1.1 Methods Notes: 1. If Potassium is present a cloudy solution will appear. Single particles are not necessarily caused by Potassium. Reagent Form of reagent/Quantity Order-No. Potassium T Tablet / 100 4515670 AL 800 / SpectroDirect_8 05/2017...

  • Page 262: S Abs, Spectral Absorption Coefficient

    1.1 Methods Spectral Absorption Coefficient (S Abs) 0 – 50 m Spectral Absorption Coefficient at 436 nm (S Abs1) Spectral Absorption Coefficient at 525 nm (S Abs2) Spectral Absorption Coefficient at 620 nm (S Abs3) Methods 345, 346 and 347 are called up one after the other and the water sample is analysed using the tests as described below: Sample preparation:...
  • Page 263 1.1 Methods Notes: 1. Filter the deionised water for zero calibration trough a membrane filter with a pore width of 0.45 μm. 2. The test complies with standard EN ISO 7887 : 1994, main section 3. 3. As colorations depend on pH and temperature, these parameters should be determined together with optical measurement and specified along with the result.

  • Page 264: Silica

    1.1 Methods Silica/Silicon dioxide with Tablet 0.05 – 3 mg/l SiO 1. Fill a clean vial (24 mm ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 265 1.1 Methods Notes: 1. The tablets must be added in the correct sequence. 2. Phosphate ions do not interfere under the given reaction conditions. 3. Conversion: mg/l Si = mg/l SiO x 0.47 Reagent Form of reagent/Quantity Order-No. Tablet / per 100 4517671BT SILICA No.

  • Page 266: Silica Lr Pp

    1.1 Methods Silica LR / Silicon dioxide LR with Vario Powder Pack and Liquid Reagent 0.1 – 1.6 mg/l SiO Use two clean vials (24 mm Ø) and mark one as blank for zeroing. 1. Fill each vial with 10 ml of water sample. 2.
  • Page 267 1.1 Methods prepare Zero 11. Press ZERO key (blank is already placed in the sample press ZERO chamber – see point 8). Countdown Wait for a reaction period of 2 minutes. 2:00 After the reaction period is finished the zero-reading starts automatically.

  • Page 268: Silica Hr Pp

    1.1 Methods Silica HR / Silicon dioxide HR with Vario Powder Pack 1 – 100 mg/l SiO 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample (Note 1), close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 269 1.1 Methods Notes: 1. Temperature of the sample should be 15°C – 25°C. 2. If Silica or Phosphate is present a yellow colour is developed 3. In this step any yellow colour due to Phosphate is removed. 4. Interferences: Substance Interference Iron large amounts interfere...

  • Page 270: Sulfate (Powder Pack)

    1.1 Methods Sulfate with Vario Powder Pack 2 – 100 mg/l SO 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 271 1.1 Methods Note: 1. If Sulfate ions are present a cloudy solution will appear. Reagent Form of reagent/Quantity Order-No. VARIO Sulpha 4 / F10 Powder Pack / 100 4532160 AL 800 / SpectroDirect_8 05/2017...

  • Page 272: Sulfide

    1.1 Methods Sulfide with Tablet – 0.04 – 0.5 mg/l S 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 273 1.1 Methods Notes: 1. The tablets must be added in the correct sequence. 2. Chlorine and other oxidizing agents which react with DPD do not interfere with the test. 3. To avoid loss of Sulfide collect the sample carefully with a minimum of aeration. It is essential to test the sample immediately after collection.

  • Page 274: Sulfite

    1.1 Methods Sulfite with Tablet 0.1 – 10 mg/l SO 1. Fill a clean 10 mm cell with water sample. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.
  • Page 275 1.1 Methods Notes: Reagent Form of reagent/Quantity Order-No. SULFITE LR Tablet / 100 4518020BT AL 800 / SpectroDirect_8 05/2017...

  • Page 276: Sulfite

    1.1 Methods Sulfite with Tablet 0.05 – 4 mg/l SO 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 277 1.1 Methods Notes: Reagent Form of reagent/Quantity Order-No. SULFITE LR Tablet / 100 4518020BT AL 800 / SpectroDirect_8 05/2017...

  • Page 278: Surfactants, Anionic (No. 1.14697.0001)

    1.1 Methods Surfactants, anionic with MERCK Spectroquant ® Cell Test, No. 1.14697.0001 0,05 – 2 mg/l MBAS Use two clean Reagent tubes and mark one as blank for zeroing. 1. Add 5 ml deionised water in the vial marked as blank Ø...
  • Page 279 1.1 Methods 9. Swirl the vial (the sample) and than place the vial (the sample) in the sample chamber making sure that the marks are aligned. (note 8) Zero accepted 10. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l MBAS. Notes: 1.

  • Page 280: Surfactants, Anionic (No. 1.02552.0001)

    1.1 Methods Surfactants, anionic with MERCK Spectroquant ® Cell Test, No. 1.02552.0001 0.05 – 2 mg/l SDSA 0.06 – 2.56 mg/l SDBS 0.05 – 2.12 mg/l SDS 0.08 – 3.26 mg/l SDOSSA Use two clean Reagent tubes and mark one as blank for zeroing.
  • Page 281 1.1 Methods 9. Swirl the vial (the sample) and than place the vial (the sample) in the sample chamber making sure that the marks are aligned. (note 7) Zero accepted 10. Press TEST key. prepare Test press TEST The result is shown in the display in mg/l SDSA. Notes: 1.

  • Page 282: Surfactants, Nonionic (No. 1.01787.0001)

    1.1 Methoden Surfactants, nonionic with MERCK Spectroquant ® Cell Test, No. 1.01787.0001 0.1 – 7.5 mg/l Triton X-100 ® 0.11 – 8.25 mg/l NP 10 Use two clean Reagent tubes and mark one as blank for zeroing. Ø 16 mm 1.
  • Page 283 1.1 Methoden Notes: 1. This method is adapted from MERCK. 2. Before performing the test read the original test instructions (delivered with the test) and the MSDS (available at www.merckmillipore.com). 3. Spectroquant is a registered trade mark of the company MERCK KGaA. ®...

  • Page 284: Surfactants, Cationic (No. 1.01764.0001)

    1.1 Methods Surfactants, cationic with MERCK Spectroquant ® Cell Test, No. 1.01764.0001 0.05 – 1.5 mg/l CTAB Use two clean Reagent tubes and mark one as blank for zeroing. 1. Add 5 ml deionised water in the vial marked as blank (this is the blank, note 6).
  • Page 285 1.1 Methods Notes: 1. This method is adapted from MERCK. 2. Before performing the test read the original test instructions (delivered with the test) and the MSDS (available at www.merckmillipore.com). 3. Spectroquant is a registered trade mark of the company MERCK KGaA. ®...

  • Page 286: Suspended Solids

    1.1 Methods Suspended Solids 0 – 750 mg/l TSS Sample preparation: Blend approx. 500 ml of the water sample in a blender at high speed for 2 minutes. 1. Fill a clean 50 mm cell with deionised water. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 287 1.1 Methods Note: 1. The photometric determination of Suspended Solids is based on a gravimetric method. In a lab this is usually done by evaporation of the filter residue of a filtrated water sample in an oven at 103°C – 105°C and weighing of the dried residue. 2.

  • Page 288: Toc Lr

    1.1 Methods TOC LR with MERCK Spectroquant ® Cell Test, No. 1.14878.0001 5.0 – 80.0 mg/l TOC Use two clean suitable glass containers and mark one as blank for zeroing. 1. Fill a clean glass container with 25 ml of deionised water (this is the blank).
  • Page 289 1.1 Methods 10. Heat vials, standing on its head, at 120°C in the pre- heated reactor for 120 minutes. 11. Wait for 1 hour before proceeding. Do not cool down with water! After cooling, turn the cell upright and measure in the photometer within 10 min.

  • Page 290: Toc Hr

    1.1 Methods TOC HR with MERCK Spectroquant ® Cell Test, No. 1.14879.0001 50 – 800 mg/l TOC Use two clean suitable glass containers and mark one as blank for zeroing. 1. Fill a clean glass container with 10 ml of deionised water (this is the blank).
  • Page 291 1.1 Methods 9. Immediately close the vials tightly with an aluminium cap. 10. Heat vials, standing on its head, at 120°C in the pre- heated reactor for 120 minutes. 11. Wait for 1 hour before proceeding. Do not cool down with water! After cooling, turn the cell upright and measure in the photometer within 10 min.

  • Page 292: Turbidity

    1.1 Methods Turbidity 5 – 500 FAU 1. Fill a clean 50 mm cell with deionised water. 2. Place the cell in the sample chamber making sure that the positioning is correct. prepare Zero 3. Press ZERO key. press ZERO 4.
  • Page 293 1.1 Methods Note: 1. This test uses an attenuated radiation method for the reading of FAU (Formazin Attenuation Units). The results can not be used for USEPA reporting purposes, but may be used for routine measurements. The attenuated radiation method is different from the Nephelometric method.

  • Page 294: Urea

    1.1 Methods Urea with Tablet and Liquid Reagent 0.1 – 2 mg/l (NH CO / mg/l Urea 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample, close tightly with the cap. 2. Place the vial in the sample chamber making sure that marks are aligned.
  • Page 295 1.1 Methods 14. Close the vial tightly with the cap and swirl several times until the tablets are dissolved. 15. Place the vial in the sample chamber making sure that marks are aligned. Zero accepted prepare Test 16. Press TEST key. press TEST Wait for a reaction period of 10 minutes.

  • Page 296: Zinc

    1.1 Methods Zinc with Tablet 0.02 – 0.9 mg/l Zn 1. Fill a clean vial (24 mm Ø) with 10 ml of the water sample. 2. Add one COPPER / ZINC LR tablet straight from the foil to the water sample, crush the tablet using a clean stirring rod.
  • Page 297 1.1 Methods Notes: 1. The tablets must be added in the correct sequence. 2. In the case of high levels of residual chlorine, perform the analysis with a dechlorinated water sample. To dechlorinate add one DECHLOR tablet to the water sample (point 1). Crush and mix to dissolve the tablet.

  • Page 298: Important Notes

    1.2 Important notes 1.2.1 Correct use of reagents The reagents must be added in the correct sequence. Tablet reagents: The tablet reagents should be added to the water sample straight from the foil without touching them with the fingers. Liquid reagents: Add drops of the same size to the water sample by holding the bottle vertically and squeezing slowly.

  • Page 299: Cleaning Of Vials And Accessories For Analysis

    1.2.2 Cleaning of vials and accessories for analysis Vials, caps and stirring rods should be cleaned thoroughly after each analysis to prevent interferences. Procedure: Clean vials and accessories after each analysis as soon as possible. 1. Clean vials and accessories with laboratory detergent (e.g. Extran MA 02 (neutral, ®...

  • Page 300: Guidelines For Photometric Measurements

    1.2.3 Guidelines for photometric measurements 1. Vials, caps and stirring rods should be cleaned thoroughly after each analysis to prevent interferences. Even minor reagent residues can cause errors in the test result. 2. The outside of the vial must be clean and dry before starting the analysis. Clean the outside of the vials with a towel.

  • Page 301: Sample Dilution Techniques

    1.2.4 Sample dilution techniques Proceed as follows for accurate dilutions: Pipette the water sample (see table) into a 100 ml volumetric flask and fill up to 100 ml mark with deionised water. Swirl to mix the contents. Water sample Multiplication [ml] factor Pipette the required volume of the diluted sample into the vial and proceed as described in...
  • Page 302 AL 800 / SpectroDirect_8 05/2017...

  • Page 303: Part 2 Instrument Manual

    Part 2 Operating manual AL 800 / SpectroDirect_8 05/2017...

  • Page 304: Operation

    2.1 Operating 2.1.1 Commissioning Before using the photometer SpectroDirect it is necessary to insert two batteries. Before using the photometer (PCSpectroII and SpectroDirect) perform the following settings in the Mode-Menu: • MODE 10: select language • MODE 12: set date and time •...

  • Page 305: Cell Chamber And Cells

    The lithium battery saves data (stored results and photometer setting) if there is no power from the power supply from the mains adapter. As long as the instrument is energised from the mains adapter the battery not discharged. Recommendation: Exchange of the lithium battery every 5 years. When no mains adapter supplies energy to the instrument, all stored data and settings will be lost, if the lithium battery is taken out.

  • Page 306: Overview Of Function Keys

    2.2 Overview of function keys 2.2.1 Overview Switching the photometer on or off Returning to selection of methods or previous menu Function key: description in the text if key available Function key: description in the text if key available Function key: description in the text if key available Confirming Mode Menu of photometer settings and further functions...

  • Page 307: User Countdown

    2.2.3 User countdown With this function the operator is able to define his own countdown. Press [“clock”] key. The display shows time and date: 19.30.20 2009-06-15 Press [“clock”] key. The display shows: Countdown mm : ss Either press [ ] key to accept the last used user count- 99 : 99 down.

  • Page 308: Operation Mode

    2.3 Operation mode If the photometer is connected with power supply unit to the mains it is ready for use. Before each start, make sure that the sample chamber is empty and the photometer lid is closed, as the photometer always performs a selftest when it is switched on. Switch the photometer on by pressing the ON/OFF key.

  • Page 309: Selecting A Method

    2.3.2 Selecting a method >> 30 Alkalinity-m The display shows a selection: 35 Alkalinity-p 40 Aluminium ..There are two possibilities to select the required method: a) enter method-number directly e.g.: [8] [0] to select Bromine b) press [ ] or [ ] key to select the required method from the displayed list.

  • Page 310: Differentiation

    2.3.3 Differentiation Chlorine >> diff Differentiation is possible in some methods (e.g. Chlorine). free The photometer then requires the type of determination. total Press arrow key [ ] or [ ] to select the required determi- nation. Confirm with [ ] key. 2.3.4 Performing Zero prepare Zero The display shows:...

  • Page 311: Performing Tests

    2.3.5 Performing Tests When zero calibration is complete, remove the vial from the sample chamber and perform the tests as described under “Method”. When the results have been displayed: - with some methods you can change between different chemical species - you can store and/or print out the results - perform further analysis with the same zero - select a new method...

  • Page 312: Changing Chemical Species

    2.3.7 Changing chemical species For some methods there is a possibility to change the chemical species of the test result. If the test result is displayed press arrow key [ ] or [ ]. Example: 320 Phosphate LR T -----[ ]----> 320 Phosphate LR T <---- [ ] ----- 320 Phosphate LR T 0.05-4 mg/l PO 0.02-1.3 mg/l P...

  • Page 313: Printing Results

    Note: Storage: 900 The display shows the number of free data sets. free records left If there are less than 30 data sets free the display shows: Storage: only 29 free records left Clear the memory as soon as possible (see “Deleting stored results”).

  • Page 314: Perform Additional Measurements

    2.3.10 Perform additional measurements To perform additional tests using the same method: Test Zero accepted • Press TEST key prepare Test press TEST The display shows: Confirm with TEST key Test • Press ZERO key to perform a new zero calibration. Zero prepare Zero The display shows:...

  • Page 315: Photometer Settings: Table Of Mode Functions

    2.4 Photometer settings: Table of MODE Functions MODE-Function Description Page Abs / Trans Measurement of Extinction and Transmission at selected wavelength Calibration Fluoride calibration Clear calibration Deleting user calibration Clock Setting date and time Countdown Switching the countdown on/off to ensure reaction times Delete data Deleting all stored results...

  • Page 316: Blank Because Of Technical Requirements

    Stor., method Displaying only results of one selected method System info Information about the instrument e.g. current software version Temperature Selection of °C or °F for Langelier Mode 70 User calibration Storage of user calibration User concentration Entering the data necessary to run a user concentration method User polynoms Entering the data necessary...

  • Page 317: Setting Date And Time

    Key beep Press [MODE] [1] [1] keys. Mode Confirm with [ ] key. <Key Beep> The display shows: ON: 1 OFF: 0 • Press [0] key to switch the key beep off. • Press [1] key to switch the key beep on. Confirm with [ ] key.
  • Page 318 Countdown (Ensuring reaction periods) Some methods require a reaction period. This reaction period is incorporated in the method as standard with the countdown function. It is possible to switch the countdown off for all methods: Mode Press [MODE] [1] [3] keys. Confirm with [ ] key.
  • Page 319 Signal beep Performing a zero or a measurement takes 8 seconds. The photometer indicates the end of zeroing or measuring by a short beep. Mode Press [MODE] [1] [4] keys. Confirm with [ ] key. <Signal Beep> The display shows: ON: 1 OFF: 0 •...

  • Page 320: Printing Of Stored Results

    2.4.3 Printing of stored results Printing all results Mode Press [MODE] [2] [0] keys. Confirm with [ ] key. <Printing> The display shows: Print all Data Start: cancel: Press [ ] key for printing out all stored test results. Test No.: The display shows e.g.: After printing the photometer goes back to mode menu automatically.
  • Page 321 Printing results of a selected time period Mode Press [MODE] [2] [1] keys. Confirm with [ ] key. <Print> The display shows: sorted: date Enter year, month and day for the first day of the required from yy-mm-dd period, e.g.: 14 May 2009 = [0][9][0][5][1][4] __-__-__ Confirm with [ ] key.
  • Page 322 Printing results of a selected Code No. range Mode Press [MODE] [2] [2] keys. Confirm with [ ] key. The display shows: <Print> sorted: Code No. Enter numeric code number (up to 6 places) for the first from _ _ _ _ _ _ required Code No., e.g.: [1].
  • Page 323 Printing results of one selected method Mode Press [MODE] [2] [3] keys. Confirm with [ ] key. The display shows: <Print> >>20 Acid demand 30 Alkalinity-total Select the required method from the displayed list or enter 40 Aluminium T the method number directly. Confirm with [ ] key.
  • Page 324 Printing Parameter Mode Press [MODE] [2] [9] keys. Confirm with [ ] key. <printing parameter> 1: Flow control 2: Baud rate The display shows: cancel: Press [1] key to select “Flow control”. <Flow Control> is: Hardware The display shows: select: [ ] [ ] save: cancel:...

  • Page 325: Recall / Delete Stored Results

    Press arrow key [ ] or [ ] to select the required baud rate. (600, 1200, 2400, 4800, 9600, 14400, 19200) Confirm with [ ] key. End with ESC key. Back to mode menu with ESC key. Back to method selection with ESC key. Note: Select “Hardware”...
  • Page 326 Recall results of a selected time period Mode Press [MODE] [3] [1] keys. Confirm with [ ] key. <Storage> The display shows: sorted: date from yy-mm-dd Enter year, month and day for the first day of the required __-__-__ period, e.g.: 14 May 2009 = [0][9][0][5][1][4]. Confirm with [ ] key.
  • Page 327 Recall results of a selected Code No. range Mode Press [MODE] [3] [2] keys. Confirm with [ ] key. The display shows: <Storage> sorted: Code-No. Enter numeric code number (up to 6 places) for the first from _ _ _ _ _ _ required Code No., e.g.: [1].
  • Page 328 Recall results of one selected method Mode Press [MODE] [3] [3] keys. Confirm with [ ] key. <Storage> The display shows: >>20 Acid demand Select the required method from the displayed list or enter 30 Alkalinity-total the method number directly. 40 Aluminium T Confirm with [ ] key.

  • Page 329: Calibration

    Delete stored results Press [MODE] [3] [4] keys. Mode Confirm with [ ] key. <Delete data> The display shows: Delete all data? YES : 1 NO : 0 • Press [0] key to retain the data sets in memory. • After pressing key [1] the following acknowledgment is displayed: <Delete data>...
  • Page 330 3. Press ZERO key. 4. Remove the vial from the sample chamber. 5. Add exactly 2 ml SPADNS reagent solution to the water sample. Caution: Vial is filled up to the top! 6. Close the vial tightly with the cap and swirl gently several times to mix the contents.

  • Page 331: User Calibration

    User Calibration If a test method is user calibrated the method name is displayed inverse. Procedure: • Prepare a standard of known concentration and use this standard instead of the sample according to the test procedure. • It is recommend to use well known standards which are formulated according to DIN EN, ASTM or other international norms or to use certified standards which are commercially available.
  • Page 332 Store user calibration 100 Chlorine T Perform the required method as described in the manual 0.02-6 mg/l Cl2 using a standard of known concentration instead of the 0.90 mg/l free Cl2 water sample. If the test result is displayed press [MODE] [4] [5] keys and Mode confirm with [ ] key.
  • Page 333 Delete user calibration This chapter only applies for methods which can be user calibrated. Chlorine T Select the required method. 0.02-6 mg/l Cl2 Instead of zeroing the instrument press [MODE] [4] [6] keys prepare ZERO and confirm with [ ] key. press ZERO Mode The display shows:...

  • Page 334: Lab Function

    2.4.6 Lab function Reduced operator guidance => “Profi-Mode” This function may be used for routine analyses with many samples of one method. The following information is always stored in the methods: a) Method b) Range c) Date and time d) Differentiation of results e) Detailed operator instruction Compliance with reaction periods If the Profi-Mode is active, the photometer provides only a minimum of operator instructions.

  • Page 335: Absorption / Transmission

    Absorption / Transmission Press [MODE] [5] [1] keys. Mode Confirm with [ ] key. < Abs / Trans > The display shows: wavelength: _ _ _ nm Select wavelength between 330 and 900 nm e.g.: [5] [4] [0] and confirm with [ ] key. wavelength set The display shows: and then:...

  • Page 336: Spectrum (Scan)

    Spectrum (Scan) A wavelength scan can be performed in the range of 330 to 900 nm. The minimum range between start and stop wavelength is 10 nm. Mode Press [MODE] [5] [3] keys. Confirm with [ ] key. < Spectrum > The display shows: Start: _ _ _ nm Enter start wavelength:...
  • Page 337 1.10 The display shows the graphic of the measured spectrum. 0.00 Press F1 key to switch between the graphic and the results (peaks & valleys). P: 460 nm 1.000 E Now the display shows the calculated peaks (P) and valleys V: 555 nm 0.951 E (V).

  • Page 338: Kinetic

    Kinetic With method 54 Kinetic it is possible to describe the time dependency of a reaction (e.g. time of colour development). The maximum number of measuring points is 199 with an interval time of 6 to 999 seconds. Concentrations of unknown samples can be calculated by a known factor.
  • Page 339 Enter the number of intervals in the range from 2 to 199 sec., Number of intervals: e.g. [1] [5]. Confirm with [ ] key. _ _ _ Note: The complete time of measurement can be calculated as product of the number of intervals with the interval time plus time delay.
  • Page 340 Option: Standard Standard: _ _ _ _ _ Enter the concentration of the standard with max. 3 digits after decimal point, e.g. [2] [.] [5]. Confirm with [ ] key. initialisation of After confirmation the standard measurement starts auto- measurement matically (see ‘Flow of Standard/Sample Measurement’).
  • Page 341 The display shows: Measurement: i / n Result: y • number of the last measurement (i) / total number of Interval time: x s measurements (n) t: a / g • the result of the last measurement (y), (Note 1) •...

  • Page 342: User Operations

    2.4.7 User operations User method list After switching on the instrument a scroll list of all available methods is automatically shown in the display. To shorten this list according to the requirements of the user it is possible to create a user defined scroll list. After performing the update successfully new methods are displayed in the user-method list automatically.
  • Page 343 User method list, switch all methods on This mode function activates all methods. After switching on the instrument a scroll list of all available methods is automatically shown in the display. Mode Press [MODE] [6] [1] keys. Confirm with [ ] key. <Mlist all on>...

  • Page 344: User Concentration Methods

    User Concentration Methods It is possible to enter and store up to 10 User Concentration Methods. Therefor you need 2 to 14 standards of known concentration and one blank (deionised water or reagent blank value). The Standards should be measured with increasing concentrations and from the brightest to the darkest colouration.
  • Page 345 Press the appropriate numerical key to select the required choose resolution resolution, e.g.: [3] for 0.01. 1: 1 2: 0.1 Note: 3: 0.01 Please enter the required resolution according to the in- 4: 0.001 strument pres-sets: range max. resolutions 0.000 ...9.999 0.001 10.00 ...99.99 0.01...

  • Page 346: User Polynomials

    S2: 0.10 mg/l Prepare the second standard and press [Test] key. prepare press TEST The display shows the input value and the measured ab- sorption value. Confirm with [ ] key. S2: 0.10 mg/l 0.15 Note: • Perform as described above to measure further stan- S2 accepted dards.
  • Page 347 Confirm with [ ] key. Note: Overwrite polynom? if the entered number has already been used to save a YES: 1, NO: 0 polynomial the display shows the query: • Press [0] or [ESC] key to go back to method No. query. •...
  • Page 348 Press [ ] or [ ] keys to select the required unit. choose unit: >> mg/l mmol/l mAbs µg/l Confirm with [ ] key. choose resolution Press the appropriate numerical key to select the required 1: 1 resolution, e.g.: [3] for 0.01. 2: 0.1 3: 0.01 Note:...

  • Page 349: Delete User Methods

    Delete User Methods (Polynomial or Concentration) In principle a valid user method can be overwritten. An existing user method (Polynomial or Concentration) can be totally deleted as well and is removed out of the method selection list: Mode Press [MODE] [6] [6] keys. Confirm with [ ] key.

  • Page 350: Print Data Of User Methods

    Print Data of User Methods (Polynomials & Concentration) With this Mode function all data (e.g. wavelength, unit ...) of stored user polynomials and concentration methods can be printed out or transferred with HyperTerminal to a PC. Mode Press [MODE] [6] [7] keys. Confirm with [ ] key.

  • Page 351: Initialise User Method System

    Initialise User Method System (Polynomials & Concentration) Power loss will cause incoherent data. The user method system must be initialised with this mode function to set it to a predefined state. ATTENTION: All stored user methods (polynomial & concentration) are deleted with initialisation. Mode Press [MODE] [6] [9] keys.

  • Page 352: Special Functions

    2.4.8 Special functions Langelier Saturation Index (Water Balance) For calculation the following tests are required: • pH value • Temperature • Calcium hardness • Total Alkalinity • TDS (Total Dissolved Solids) Run each test separately and note the results. Calculate the Langelier Saturation Index as described: Calculation of Langelier Saturation Index With Mode 71 (see below) it is possible to select between degree Celsius or degree Fahrenheit.
  • Page 353 pH value The display shows: 0<=pH<=12 +_ _ _ _ Enter the pH-value in the range between 0 and 12 and confirm with [ ] key. <Langelier> Langelier The display shows the Langelier Saturation Index. saturation index 0.00 Press [ ] key to start new calculation. Return to mode menu by pressing [ESC] key.

  • Page 354: Instrument Basic Settings 2

    2.4.9 Instrument basic settings 2 Adjusting display contrast Press [MODE] [8] [0] keys. Mode Confirm with [ ] key. <LCD contrast> The display shows: • Press [ ] key to increase contrast of the LCD display. • Press [ ] key to decrease contrast of the LCD display. Confirm with [ ] key.

  • Page 355: Instrument Special Functions /Service

    2.4.10 Instrument special functions /service Photometer Information Mode Press [MODE] [9] [1] keys. Confirm with [ ] key. This method informs you about the current software version, <System-Info> Software: about the current detected mains power supply, about the number of performed tests and free memory capacity. V012.002.3.003.002 more: , cancel: Esc...

  • Page 356: Data Transfer

    2.5 Data transfer Switch the photometer and the personal computer or printer off. Connect the RS232 interface of the photometer (D) and the serial interface of the personal computer or printer using a cable in line with the specified assignment (see technical data). The cable for connection to a personal computer is included in delivery contents.

  • Page 357: Connection To A Printer

    2.5.1 Connection to a printer Printer with a serial connection are suitable for connection with the photometer (see chapter 3.4 Technical data interface). A suitable paper tabel printer is the printer DP 1012 or the printer DPN 2335. Before using the printer DP 1012 with the Photometer you should change the following standard adjustments: (Detailed information of changing the adjustment you will find in the printer manual).

  • Page 358: Internet Updates

    2.5.3 Internet Updates It is possible to update new software applications and additional languages via internet. Please find detailed information at our homepage in the download-area. The magnet part of the delivery contents is needed for updating the instrument! Remark: To prevent loss of stored test results store or print them out before performing an Update.

  • Page 359: Part 3 Enclosure

    Part 3 Enclosure AL 800 / SpectroDirect_8 05/2017...

  • Page 360: Unpacking

    3.1 Unpacking Carefully inspect all items to ensure that every part of the list below is present and no visible damage has occurred during shipment. If there is any damage or something is missing, please contact your local distributor immediately. 3.2 Delivery contents Standard contents for PC Spectro II / SpectroDirect: 1 Photometer...

  • Page 361: Technical Data

    3.4 Technical data Display Graphic Display (7-line, 21-characters) Serial Interface RS232 for printer- and PC-connection; 9-pin D-sub-mail connector, data format ASCII, 8-bit data, no parity, 1 start-bit, 1 stop-bit, baud rate and protocol: adjustable Pin assignation: Pin 1 = free Pin 6 = free Pin 2 = Rx Data Pin 7 = RTS...

  • Page 362: Abbreviations

    3.5 Abbreviations Abbreviation Definition °C degree Celsius (Centigrade) °F degree Fahrenheit °F = (°C x 1.8) + 32 °dH degree German Hardness °fH degree French Hardness °eH degree English Hardness °aH degree American Hardness Absorption unit ( = Extinction E) 1000 mAbs = 1 Abs = 1 A = μg/l...

  • Page 363: Troubleshooting

    3.6 Troubleshooting 3.6.1 Operating messages in the display / error display Display Possible Causes Elimination Overrange reading is exceeding the range if possible dilute sample or use water sample is too cloudy other measuring range filtrate too much light on the photo cell water sample Underrange result is under the detection limit...
  • Page 364 Display Possible Causes Elimination Test procedure correct? The calculation of a value (e.g. combined Chlorine) is If not – repeat test not possible Example 1 Example 1: The readings for free and total 0.60 mg/l free Cl Chlorine are different, but comb Cl considering the tolerances of 0.59 mg/l total Cl...

  • Page 365: General

    3.6.2 General Finding Possible Causes Elimination Test result deviates from Chemical species not as Press arrow keys to select the the expected required required chemical species. No differentiation: Profi-Mode is switched on Switch Profi-Mode off with e.g. for the Chlorine Mode 50.

  • Page 366: Service / Maintains

    3.6.3 Service / Maintains 3.6.3.1 Handling & Cleaning • Use the instrument only under ambient environmental conditions (e.g. no extreme heat, dust, humidity). • For cleaning the instrument just use a damp tissue, no solvents. Always close the cell chamber lid to protect the optics. •...

  • Page 367: Changing The Lithium Battery (Only Pc Spectro Ii)

    3.6.3.3 Changing the lithium battery (only PC Spectro II) 1. Switch the instrument off. 2. Unscrew the front plate (1). 3. Remove the front plate carefully lifting it up and pushing it backwards (2-4). Attention: Don’t destroy the cable connection. 4.

  • Page 368: Changing The Batteries (Only Spectrodirect)

    3.6.3.4 Changing the batteries (only SpectroDirect) 1. Switch the instrument off. 2. Open the battery cover (B). 3. Remove the old batteries. 4. Place the new batteries into the holder with the correct polarity. 5. Put the cover back into position and seize it. Caution: Dispose batteries according to the local regulations.

  • Page 369: Declaration Of Ce-Conformity

    3.7 Declaration of CE-Conformity Declaration of CE-Conformity The manufacturer: Tintometer GmbH Schleefstraße 8-12 44287 Dortmund Deutschland declares that this product Product name: Aqualytic AL800 / SpectroDirect ® The product above mentioned is in compliance with: European Union Council Directive of may, 3rd, 1989 regarding the reconciliation of union members legislations relative to Electromagnetic Compatibility (89/336/CEE) (JOCE 23.05.89 L 139/19-26).
  • Page 370 Declaration of CE-Conformity Tintometer GmbH The manufacturer: Schleefstraße 8a 44287 Dortmund Deutschland declares that this product Aqualytic Product name: ® spectro The product above mentioned is in compliance with: European Union Council Directive of may, 3rd, 1989 regarding the reconciliation of union members legislations relative to Electromagnetic Compatibility (89/336/CEE) (JOCE 23.05.89 L 139/19-26).
  • Page 372 Tintometer GmbH, Division Aqualytic ® Schleefstraße 8-12 | 44287 Dortmund | Germany Tel.: (+49) (0)2 31 / 9 45 10-755 | Fax: (+49) (0)2 31 / 9 45 10-750 Technical changes without notice sales@aqualytic.de | www.aqualytic.de Printed in Germany 05/17...

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