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ChromTech UV-3000 Manual

Uv-3 series
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..................................................................................... 1
General ..................................................................................
Electrical ................................................................................
Warning ....................................................................................
Unpacking Instructions ............................................................
.......................................................................
Installation ..............................................................................
Operation ...................................................................................
Turn on spectrophotometer ... ...................................................
Basic operation.....................................................................
Analyse Sample .....................................................................
Basic Mode .......................................................................
Quantitative .....................................................................
WL Scan ...........................................................................
Kinetics .................................................................................. 25
........................................................................ 28
Multi Wavelength ....................................................................
.................................................................................
Appendix C
Lamp replacement................................................................
Replace W lamp................................................................44
Replace D2 lamp............................................................. 45
Battery replacement................................................................
Trouble shooting...................................................................... 47
Contents
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UV-3 SERIES SPECTROPHOTOMETER
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Summary of Contents for ChromTech UV-3000

  • Page 1: Table Of Contents

    UV-3 SERIES SPECTROPHOTOMETER Contents SAFETY ………………………………………………………………….……… 1 General ………………………………………………………………………. Electrical …………………………………………………………………….. Warning ………………………………………………………………………… Working Principle ………………………………………………………… Unpacking Instructions …………………………………………………… Specifications ……………………………………………..……………… Installation …………………………………………………………………… Operation ………………………………………………………..……………… Prepare the Spectrophotometer ………………………………………… 4 Description of keys ………… …………………………………… Turn on spectrophotometer … …………………………………………… Basic operation……………………………………………………………...

  • Page 2: Safety

    There are no serviceable components inside this power supply unit. For ChromTech UV-3 SERIES, avoid touching the high voltage power supply at all times.

  • Page 3: Working Principle

    Whenever it is likely that the protection has been impaired, the apparatus shall be made inoperative and be secured against any unintended operation. NEVER touch or handle the power supply on ChromTech UV-3 SERIES due to the high voltage. The protection is likely to be impaired if, for example, the apparatus...

  • Page 4: Specifications

    UV-3 SERIES SPECTROPHOTOMETER Specifications: UV-3000/ UV-3100/ UV-3200/ UV-3200S/ UV-3300/ Model UV-3000PC UV-3100PC UV-3200PC UV-3200PCS UV-3300PC Wavelength Range 190-1100nm 4.0nm 2.0nm 1.8nm 1.0nm Spectral Bandwidth 0.5/1.0/2.0/4.0nm Optical System Single Beam, Grating 1200lines/mm Wavelength Accuracy ±0.5nm ±0.3nm Wavelength 0.3nm 0.2nm Repeatability Scanning Speed Hi, Med.,Low.Max.3000nm/min...

  • Page 5: Operation

    UV-3 SERIES SPECTROPHOTOMETER 3. Remove any obstructions or materials that could hinder the flow of air under and around the instrument. 4. Use the appropriate power cord and plug into a grounded outlet. Fuse Power Switch Power Cord Parallel Port USB Port Fig.1-4 5.

  • Page 6: Description Of Keys

    UV-3 SERIES SPECTROPHOTOMETER FUNCTION CLEAR NUMBER +/-/. PQRS WXYZ 100%T OPEN SAVE OABS CONTROL START GOTO λ PRINT ENTER STOP Fig 2 Description of keys Exit to previous screen or cancel the operation; 【ESC】 【100%T /0Abs】 Blank or scan the base line; open data or curve saved before;...

  • Page 7: Turn On Spectrophotometer

    UV-3 SERIES SPECTROPHOTOMETER Change “x” scale; Search point after scan; 【＜】 clear a 【＜】,【＞】 character; Change “y” scale; Search peak after scan; Scroll items for 【∧】,【∨】 selecting; Change capital/small letter last typed in; Browse the items for selection; Turn on spectrophotometer Turn on spectrophotometer by pressing the Power Switch (IO).

  • Page 8: Basic Operation

    UV-3 SERIES SPECTROPHOTOMETER WL : 656.1nm 16:20:18 Wait until EasyRTOS booted: Warm up 15 minutes..√ System calibrating? Fig 4 WL : 656.1nm Step to 190nm Wait until EasyRTOS booted: Search 656.1nm ..√ Check Energy.
  • Page 9 UV-3 SERIES SPECTROPHOTOMETER WL : 656.1nm Energy Low. . . Blanking Cell Max E Unit Mode Factor Standard Fig 7 NOTE: 1.Blanking is automatic after a wavelength change DO NOT OPEN SAMPLE COMPARTMENT LID DURING BLANKING. 2. The dark current doesn’t be taken after power on, if you bypass the calibrating system.
  • Page 10 UV-3 SERIES SPECTROPHOTOMETER WL : 656.1nm 12: 35: 27 0.001 Abs Cell Max E Please input wl: 656.1 Fig 10 Press 【ENTER】 to change the wavelength from 656.1nm to 450.0nm,and then blank; After blanking, the screen displays as Fig WL : 450.0nm 12: 35: 27 0.000 Abs Cell...

  • Page 11: Quantitative

    UV-3 SERIES SPECTROPHOTOMETER WL : 680.0nm 12: 35: 27 Cell From: 200 .0 680 .0 Step: 1 .0nm XScale YScale 200.0 Wavelength (nm) 680.0 Setup Mode Search Smooth Fig 12 WL : 680.0nm 12: 35: 27 Cell From: 200 .0 680 .0 Step: 1 .0nm...
  • Page 12 UV-3 SERIES SPECTROPHOTOMETER (3) The length of filename is less than 4. WL : 680.0nm 12: 35: 27 Cell From: 200.0 680.0 Step: 1.0nm XScale YScale 200.0 Wavelength (nm) 680.0 Please input File Name:2 Fig 14 Table 2 representing representing representing 0,+,-,* ,/ 1,#,?,:,I...
  • Page 13 Fit the blank cuvette into the 4-cell linear changer and place the cuvette in the slot nearest you. For the ChromTech UV-3 SERIES, push the changer so that the cuvette is in the light path (Push the rod in). Close the lid.
  • Page 14 UV-3 SERIES SPECTROPHOTOMETER WL : 656.1nm 12: 35: 27 0.104 Abs Cell Max E Unit Mode Factor Standard Fig 19 1. Abs mode Push the blank cuvette into the light path. Press 【F2】 to select Abs mode ,Press 【0Abs/100%T】 for Blanking , and then Push the sample into light path to take reading(Fig 19 ) 2.
  • Page 15 UV-3 SERIES SPECTROPHOTOMETER light path to take reading of concentration 4.2 Push sample of known concentration into the light path Press【F4】to input known Conc value, Fig 23. Then push the sample into light path to take reading of concentration. Note:1.You can select wavelength at any time by pressing【GOTOλ】. After your selection, instrument always blanks automatically.
  • Page 16 UV-3 SERIES SPECTROPHOTOMETER WL : 700.0nm Abs : 12: 35: 27 Quantitative Test Conc . (mg/L) Cell WL(nm) 700 .0 Search Scroll C=1.000*A^1 r=1.000 Unit Fit Curve Fig 25 ☺ How to operation 1. Press 【F1】to select unit of concentration (Fig 26). WL : 700.0nm Abs: 12: 35: 27...
  • Page 17 UV-3 SERIES SPECTROPHOTOMETER WL : 700.0nm Abs : 12: 35: 27 Calibration table Conc. (mg/L) Abs Cell WL(nm) 700 .0 C=1.000*A^1 r=1.000 Method Params Standard Show Curve Fig 28 3.1 Press 【F1】 in Fig 28 to select fitting method. There are 4 methods for you to choose: Linear fit, linear fit through zero, square fit and cubic fit.
  • Page 18 UV-3 SERIES SPECTROPHOTOMETER WL : 700.0nm Abs : 12: 35: 27 Setup Standard Conc. Conc. (mg/L) Cell WL(nm) 1 0.062 0.062 700 .0 2 0.061 0.061 3 0.062 0.061 4 0.061 0.061 C=1.000*A^1 r=1.000 Edit the number . . . Fig 30 WL : 700.0nm Abs :...
  • Page 19 UV-3 SERIES SPECTROPHOTOMETER regression .r=1 is best fitting. Usually “ r ” is very close to 1. Note: If there are few standard samples, it is not suitable for selecting square fitting, especially cubic fitting, otherwise invalid fitting result will be obtained. WL : 656.1nm Abs : 12: 35: 27...
  • Page 20 UV-3 SERIES SPECTROPHOTOMETER WL : 656.1nm Abs: 12: 35: 27 Cell -1.0 Press (ESC) to return . . . Fig 37 linear fit 3.3.5 Press 【SAVE】 to save calibration if required 3.3.6 Press【ESC】to exit 4. Quantitative Test Before test, the standard curve must be obtained. There are three ways for you to obtained it (a, b or c).
  • Page 21 UV-3 SERIES SPECTROPHOTOMETER ☺ Print Test Report Press the key【PRINT】to print the test report (Fig 39). Fig 39 WL Scan Press【3】in main menu for “WL Scan” test (Fig 40). 【ESC】to exit. To open a previous curve, press【OPEN】and select a previously stored curve (.wav) WL : 656.1nm Abs :...
  • Page 22 UV-3 SERIES SPECTROPHOTOMETER and 5nm. “Scan speed” allows the selection of “HI”, “MEDIUM” and “LOW”. For survey scan we suggest 5nm, HI. For detailed scan we suggest 0.5nm, HI 2. Press 【F2】to select the test mode, ”Abs” , “%T” or ”E”’ (Fig 42). WL : 656.1nm Abs:...
  • Page 23 UV-3 SERIES SPECTROPHOTOMETER WL : 680.0nm 12: 35: 27 Cell From: 200 .0 680 .0 Step: 1 .0nm XScale YScale 200.0 Wavelength (nm) 680.0 Setup Mode Search Smooth Fig 45 If you want to change the scale, press 【＜】 or 【＞】 to change “x” scale (Fig 46), input upper limit and lower limit by pressing the numeric keypad .
  • Page 24 UV-3 SERIES SPECTROPHOTOMETER WL : 680.0nm 12: 35: 27 Cell From: 200 .0 680 .0 Step: 1 .0nm Point Peak 200.0 Wavelength (nm) 680.0 Set Peak Height Fig 48 a) Peak to peak, press 【 F1 】 to set “peak height” and input value by pressing the numeric keypad (Fig 49).
  • Page 25 UV-3 SERIES SPECTROPHOTOMETER ☺ Print Test Report Press 【PRINT】to print the curve you have opened or scanned (Fig 51). Note: The report always is printed in Fig 45 Fig 51...
  • Page 26 UV-3 SERIES SPECTROPHOTOMETER Kinetics Press【4】in main menu for “Kinetics” (Fig 52). 【ESC】to exit. To open a previous kinetics result, press 【OPEN】 and select a previously stored result (.kin) Tim : 60s Abs : 12: 35: 27 Cell Total T 180 s Inteval 1 .0s XScale...
  • Page 27 UV-3 SERIES SPECTROPHOTOMETER scan the sample. After the delay time, the beeper beeps 3 times and time -scan starts. At the end of the time-scan, the beeper also beeps 3 times (Fig 55); Tim : 180s Abs : 12: 35: 27 Cell Total T 180 s...
  • Page 28 UV-3 SERIES SPECTROPHOTOMETER 7. Press【F4】to search the Abs/%T value in relation to the time axis. Search point to point by pressing the key【＜】or【＞】. Please refer to step 6 of “WL scan”. ☺ Save Curve Press the key 【SAVE】 to save curve. Note: Open/Save requires the first kinetics display page Fig.
  • Page 29 UV-3 SERIES SPECTROPHOTOMETER DNA/Protein Press【5】in main menu for “DNA/Protein” (Fig 59). 【ESC】to exit. Note: The algorithm of the test refer to Appendix A please. WL : 900.0nm 12: 35: 27 DNA/Protein measurement Items Result Unit Cell WL(nm) 260 .0 280 .0 320 .0 Search Scroll...
  • Page 30 UV-3 SERIES SPECTROPHOTOMETER WL : 900.0nm 12: 35: 27 DNA/Protein measurement Items Result Unit Cell WL(nm) 260 .0 280 .0 Search Scroll Measument: Absorbance difference 1 Fig 61 WL : 900.0nm 12: 35: 27 DNA/Protein measurement Items Result Unit Cell WL(nm) 260 .0 280 .0...
  • Page 31 UV-3 SERIES SPECTROPHOTOMETER WL : 900.0nm Abs : 12: 35: 27 DNA/Protein measurement Items Result Unit Cell 2 .947 WL(nm) 2 .842 260 .0 Aref 0.638 280 .0 320 .0 C -DNA 65.91 mg/mL C -Pro 1672 mg/mL Ratio 1.048 Search Scroll Coeff...

  • Page 32: Multi Wavelength

    UV-3 SERIES SPECTROPHOTOMETER Multi Wavelength Press【6】in main menu for “Multi WL” (Fig 67). 【ESC】to exit. WL : 900.0nm Abs : 12: 35: 27 Multi Wavelength Test WL(nm) Cell 1 WL 500.0 Search Scroll WL Setup Mode Fig 67 【 】 To open previous Multi Wavelength results, press OPEN and select previously stored...
  • Page 33 UV-3 SERIES SPECTROPHOTOMETER WL : 900.0nm Abs : 12: 35: 27 Multi Wavelength Test WL(nm) Cell 3 WL 500.0 400.0 300.0 Search Scroll Please Select Mode: Fig69 3. Push the blank cuvette into the light path, then press 【0Abs/100%T】 for Blanking . 4.

  • Page 34: Utility

    UV-3 SERIES SPECTROPHOTOMETER Fig 71 Utility Press【7】in Main menu for “Utility” (Fig 72). 【ESC】to exit. WL : 656.1nm WL : 656 .1nm 08: 04: 35 08: 04: 35 Cell Cell System Service Routines System Service Routines 1. Wavelength Reset 6. Connect to PC 2.
  • Page 35 UV-3 SERIES SPECTROPHOTOMETER WL : 656.1nm 08: 04: 35 Cell Setup Printer 1. Reset Printer 2. Select print port 3. Select printer 4. Change print mode Move Dn Select Cancel Fig 74 Press【1】in Fig 74 to Reset Printer. Press【2】in Fig 74 to select print port (LPT or Comm., Fig 75). WL : 656.1nm 08: 04: 35 Cell...
  • Page 36 UV-3 SERIES SPECTROPHOTOMETER WL : 656.1nm 08: 04: 35 Cell Setup Printer 1. Reset Printer 2. Select print port 3. Select printer 4. Change print mode Move Dn Select Cancel Move Up Fig77 ☺ Lamp Press【3】to set lamp (Fig 78). 【ESC】to exit. WL : 656.1nm 08: 04: 35 Cell...
  • Page 37 UV-3 SERIES SPECTROPHOTOMETER WL : 656.1nm 08: 04: 35 Cell Lamps Management Switch D 2: 1. Switch on/off D 2 Reset D2 lamp usage time 2. Reset D2 lamp usage time Switch W : 3. Switch on/off W Reset W lamp usage time Switch point 4.
  • Page 38 UV-3 SERIES SPECTROPHOTOMETER WL : 656.1nm 08: 04: 35 Cell Lamps Management Switch D 2: 1. Switch on/off D 2 Reset D2 lamp usage time 2. Reset D2 lamp usage time Switch W : 3. Switch on/off W Reset W lamp usage time Switch point 4.
  • Page 39 UV-3 SERIES SPECTROPHOTOMETER 4. Press【4】in Fig 84 to set the date display on the top right corner of the WL : 656.1nm 08: 04: 35 Cell Clock Management Switch D 2: 1. Set time Reset D2 lamp usage time 2. Set date Switch W : 3.
  • Page 40 UV-3 SERIES SPECTROPHOTOMETER WL : 656.1nm 08: 04: 35 Controlled by computer . . . Press (Esc) to return... Fig 89 ☺ Beeper on/off Press【7】in Fig 72 to turn on/off the beeper ☺ Language selection Press 【8】in Fig 72 to select working language, English and Chinese are under your choice.

  • Page 41: Restore Default Settings

    UV-3 SERIES SPECTROPHOTOMETER WL : 656.1nm 08: 04: 35 Cell System Service Routines Fig 91 ☺ Restore Default Settings Move the cursor onto the eleventh menu in Fig 72, then press 【ENTER】to restore the default parameters.(Fig 92) WL : 656.1nm Initializing ...

  • Page 42: Appendix A

    UV-3 SERIES SPECTROPHOTOMETER Appendix A DNA/Protein Test Algorithm Wavelength(s) Parameters Test Name Method Calculations Displayed Units DNA MEASUREMENT Absorbance =62.9 DNA: 260nm difference DNA/Protein concentration: =36.0 μg/ml 280nm (260,280) =1552 Protein:μ 320nm Concentration Protein =757.3 (optional) g/ml concentration DNA purity Absorbance =49.1 260nm...

  • Page 43: Appendix B

    UV-3 SERIES SPECTROPHOTOMETER Appendix B A number of correction techniques can be used to eliminate or reduce interference errors. In general, if the source of the error is known and is consistent from sample to sample, the error can be eliminated. On the other hand, if the source is unknown and varies from sample to sample, the error can be reduced but not eliminated.
  • Page 44 UV-3 SERIES SPECTROPHOTOMETER because it corrects for any background absorbance that exhibits a linear relationship to the wavelength. In many cases, if the wavelength range is narrow, it will be a reasonable correction for non-linear background absorbance such as that resulting from scattering of from a complex matrix Fig A2...

  • Page 45: Lamp Replacement

    UV-3 SERIES SPECTROPHOTOMETER Appendix C Maintenance Inside Structure Radiator Lamp Chamber Compartment Mono-chromator Chamber Cell-holder Fig.4-1 Inside Planform 1. Lamp Replacement Replace Tungsten-Halogen Lamp Turn off and unplug the instrument. Remove the four screws on the sides of the spectrophotometer Remove the Cuvette Holder Control Knob by unscrewing the rod counterclockwise.

  • Page 46: Replace W Lamp

    UV-3 SERIES SPECTROPHOTOMETER Fig.4-2 CAUTION: 1) Do not handle the lamp with bare fingers. Use tissue or cloth when handling lamp. 2) There’s no difference in polarity of the two legs of Tungsten-halogen lamp. Switch the instrument on. Set the wavelength at 340 nm, insert an empty cuvette, and blank the instrument.

  • Page 47: Battery Replacement

    Reinstall the four screws and the Cuvette Holder Control Knob. 2. Battery Replacement ChromTech UV-1100 and 12 Series can save many data in the RAM, so a battery must be used in the mainboard. When the battery is disabled, you’ll have to replace it as the following indication.

  • Page 48: Trouble Shooting

    UV-3 SERIES SPECTROPHOTOMETER 3、Trouble-shooting PROBLEM Possible Cause Solution Instrument Power cord not connected to outlet Plug instrument in Dead Power outlet Change to a different outlet Inoperative Wrong voltage setting Switch to your local voltage or call after switch an authorized service engineer power on Internal fuse blown or defective Call an authorized service...

This manual is also suitable for:

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