Amersham Frac-950 User Manual
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Amersham Frac-950 User Manual

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Fraction Collector
Frac-950
User Manual
18-1139-56

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  • Page 1 Fraction Collector Frac-950 User Manual 18-1139-56...
  • Page 3 All goods and services are sold subject to the terms and All users must read this entire manual to fully conditions of sale of the company within the Amersham understand the safe use of Frac-950. Biosciences group which supplies them. A copy of these terms and conditions is available on request.

  • Page 5: Table Of Contents

    Installing a standard mode tube rack ............18 Installing a prep mode tube rack ..............18 Installing capillaries ..................19 General ......................19 Using Frac-950 with DropSync ..............19 Using Frac-950 with the accumulator ............21 Waste handling ....................23 Connecting electrical signal cables ..............24 DropSync connector ..................24 Accumulator connector ................24 Connecting to a UniNet 1 communication link ..........25...
  • Page 6 Contents ____________________________________________________ Operation On/off ......................40 Operating Frac-950 ..................41 Collecting fixed fractions ................. 42 Collecting peak fractions ................. 42 Feed tube ......................42 Ignore_LastTube ....................43 Reset_Frac_Position ..................43 AccumulatorWash ................... 44 Setting delay volume ..................45 3.10 Flow control during tube change ..............46 3.11 Define rack and tube parameters ..............

  • Page 7: Introduction

    Version 3.21 or higher is needed for standard mode, 4.0 or higher for prep mode. Frac-950 is equipped with an accumulator to eliminate spillage at high flows. A drop sensor that can be used to control tube change at low flows is also included.
  • Page 8 • Choice of six different collecting racks—four for standard mode collection and two for preparative (prep) mode. One 18/30 mm tube rack for standard mode collection is supplied with Frac-950. The others are optional accessories. • Two different methods for reducing spillage during tube change: DropSync or Accumulator.

  • Page 9: Safety

    • Operate in accordance with local safety instructions. WARNING! Pinch hazard. Do not have any part of your body within the unit base area when Frac-950 is switched on. An automatic calibration process starts when Frac-950 is connected to Pinch UNICORN.

  • Page 10: Installation

    To make it easier to move Frac-950 on the laboratory bench, first lift the front (approx. 30°) and tilt the unit until the rubber feet clear the bench.

  • Page 11: Assembling Tube Racks

    The funnel-to-flask rack is actually the prep mode 30 mm tube rack but only 30 of the 80 holes are filled with funnels. Frac-950 is delivered with rack type 18 and 30 mm tubes. The other rack models are available as accessories.
  • Page 12 Installation __________________________________________________ Rack A: 18 and 30 mm tubes, yellow Rack B: 12 mm tubes, violet Figure 2-2. Tube patterns as shown in UNICORN Method editor Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...
  • Page 13 __________________________________________________ Installation Rack C: Microplates 96 and 30 mm tubes, blue Rack D: 30 mm tubes, red Figure 2-3. Tube patterns as shown in UNICORN Method editor Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...
  • Page 14 Installation __________________________________________________ Rack E: 30 mm tubes, yellow Rack F: 250 ml bottles, green The only available option for this rack type Figure 2-4. Tube patterns as shown in UNICORN Method editor Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...
  • Page 15 __________________________________________________ Installation Rack G: 30 mm funnels, funnel-to-flask, yellow Figure 2-5. Tube patterns as shown in UNICORN Method editor Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 16: Assembling Standard Mode Racks

    Push the tube holder and tube support combination onto the bowl, noting the keying guides on the bowl. The surface of the holder should be level. All other standard mode rack models are assembled in a similar way. Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 17: Assembling Prep Mode Racks

    Fasten the two rack legs with guide pins to the bar using the supplied screws (1). Fasten all rack legs to the rack using the Phillips screws (2). Put the plugs in place (3). Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 18: Installing A Tube Rack

    Fold up the safety bar. Installing a prep mode tube rack Note: Installing the prep mode racks requires a Prep mode conversion kit. See 2.11 Changing from standard mode to prep mode. Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 19: Installing Capillaries

    Flow restrictor FR-904. Use the standard ÄKTA connector on the DropSync unit to connect the free end of the capillary to Frac-950 as shown in Figure 2-9. Loosen the knurled screw to lower the DropSync unit to improve access.
  • Page 20 System:Settings:Specials • In , change the parameter. FracParameters DelayVol The ÄKTAdesign Optional Configurations User Manual for each ÄKTAdesign system describes how to calculate the parameter values. • Select parameter. Tube DropSync TubeChange Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 21: Using Frac-950 With The Accumulator

    Flow restrictor FR-904. Connect the free end of the capillary to the lower port on the valve manifold in the delivery unit on Frac-950, see Figure 2-10. Figure 2-10. Connecting a capillary to the lower port Connect another capillary to the upper port on the valve manifold in the delivery unit on Frac-950, see Figure 2-11.
  • Page 22 UNICORN. System:Settings:Specials • In , change the parameter. The FracParameters DelayVol ÄKTAdesign Optional Configurations User Manual for each ÄKTAdesign system describes how to calculate the parameter value. • Select parameter. Accumulator TubeChange Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 23: Waste Handling

    All other system configurations use port F1 on the outlet valve for liquid waste after the column, except for peak fractionation without ordinary fractionation, during which the Frac-950 waste is used between peaks. To empty the waste tube completely, loosen the waste tube from the cable recess and lower it.

  • Page 24: Connecting Electrical Signal Cables

    Installation __________________________________________________ 2.7 Connecting electrical signal cables At delivery, the DropSync and the Accumulator cables are packed in a plastic bag. See Figure 2-13 to locate the connectors on the rear panel of Frac-950. UniNet 1 UniNet 3 UniNet 3...

  • Page 25: Connecting To A Uninet 1 Communication Link

    __________________________________________________ Installation 2.8 Connecting to a UniNet 1 communication link Frac-950 is controlled from a PC running UNICORN version 3.2 or higher using UniNet 1 cables (Prep Mode requires version 4.0). CAUTION! The mains power to the ÄKTAdesign chromatography system must be switched OFF before connecting Frac-950 to the UniNet 1 link.

  • Page 26: Connecting To A Supply Voltage

    Figure 2-16 shows connection to an ÄKTAexplorer system. To mains socket To Frac-950 Figure 2-16. Connecting Frac-950 mains supply to an ÄKTAexplorer system Note: Frac-950 contains no user replaceable fuse. Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 27: Inserting Collection Tubes

    Insert sufficient collection tubes into the rack, gently pushing each one down as far as it will go. The starting position can be freely selected. This is stated in the method editor or manually in instruction using parameter Fractionation Start at. Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 28: Changing From Standard Mode To Prep Mode

    Installation __________________________________________________ 2.11 Changing from standard mode to prep mode General Using the Frac-950 in prep mode means that the rack is fixed and that the dispenser arm moves. The prep mode is suitable for collecting extra large fraction volumes.

  • Page 29: Removing Standard Mode Components

    Tighten the Phillips screw. Fit the delivery arm lid. Remove the rack holder lid (5). Loosen and remove the rack holder retaining screw (6). Remove the rack holder (7). Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 30: Installing The Prep Mode Conversion Kit

    (2). Turn the shaft on the guide pin until it snaps firmly in position. Insert and tighten the shaft retaining screw (3). See “Assembling prep mode racks” on page 17 for details of assembling the racks. Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...
  • Page 31 Figure 2-19. Installing a prep mode rack Push the waste arm to the right (1). Place the rack on the Frac-950 (2). Use the guide pins on the rack legs to position the rack. Check that the guide pin fits (3).
  • Page 32 Fit the tip of the knurled screw to the shaft recess (2). Tighten the knurled screw. WARNING! Moving parts. The dispenser arm can make sudden rapid movements. Do not have any part of your body within the unit base area when Frac-950 is in operation. Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...
  • Page 33 (a distance between dispenser arm and the tip of the fractionation vessels of 0.5-1 cm is appropriate). Tighten the knurled screw (4). Fold up the safety bar. Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 34: Installing The Funnel-To-Flask Kit

    Install the rack. Referring to Figure 2-19 on page 31. Cut the tubing to appropriate lengths. Fit the funnels to the tubings, see Figure 2-23. Funnel Figure 2-23. Fitting a funnel to a tubing Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...
  • Page 35 Feed the tubings with the funnels through the rack holes. Organize the tubings at the tubing holders. Make sure that the tubings are not squeezed. Figure 2-24. Frac 950 with the Funnel-to-flask kit Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 36: Changing From Prep Mode To Standard Mode

    Switch off Frac-950 at the mains switch on the rear panel. Switch on Frac-950. Connect the system in UNICORN. When the system has connected to the Frac-950 again, it should be positioned at the waste position of the new mode. Fraction Collector Frac-950...

  • Page 37: Removing Prep Mode Components

    Loosen the finger-tight connector and pull out the capillary (1). Loosen the knurled screw (2). Remove the dispenser arm (3). Push the waste arm to the right (4). Lift up and remove the rack (5). Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...
  • Page 38 Remove the dispenser arm shaft as shown in Figure 2-26. Retaining screw Dispenser arm shaft Figure 2-26. Removing the dispenser arm shaft Unscrew and remove the retaining screw (1). Remove the dispenser arm shaft (2). Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 39: Installing Standard Mode Components

    0.5 cm) and turn the delivery arm forward until it snaps firmly in position. Tighten the Phillips screw and refit the lid. For installing standard mode racks, see “Assembling standard mode racks” on page 16. Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 40: Operation

    When calibration is completed, the tube holder stops in the home position. Note: The safety bar must be folded up for Frac-950 to start up. Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 41: Operating Frac-950

    ___________________________________________________ Operation 3.2 Operating Frac-950 Frac-950 is controlled from a PC running UNICORN version 3.21 for standard mode or version 4.0 or higher for prep mode. Control of Frac-950 can be achieved automatically from a method, or manually via the functions available in UNICORN.

  • Page 42: Collecting Fixed Fractions

    ÄKTAdesign Optional Configuration User Manual. 3.4 Collecting peak fractions Details about collecting peaks only using Frac-950 in a method are described in the ÄKTAdesign Optional Configuration User Manual. 3.5 Feed tube During fractionation, the tube rack can be moved forward one tube...

  • Page 43: Ignore_Lasttube

    The instruction will reset next position for all tube types. Select menu in UNICORN. System Control:Manual:Frac... Select the instruction in the list. Reset_Frac_Position Frac Click on the button. Execute Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 44: Accumulatorwash

    Select menu in UNICORN. System Control:Manual:Frac... Select the instruction in the list. AccumulatorWash Frac Select the number of strokes to be used for washing with the parameter Strokes Click on the button. Execute Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 45: Setting Delay Volume

    3.9 Setting delay volume The delay volume between the UV flow cell in the chromatographic system and Frac-950 must be known to UNICORN. The delay volume is used to adapt the collected fractions to the event marks generated by UNICORN.

  • Page 46: Flow Control During Tube Change

    • Accumulator During tube change, the flow is diverted to the accumulator which stores the liquid. When the new tube is in position, the liquid is pressed out rapidly for collection. Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 47: Define Rack And Tube Parameters

    3.11 Define rack and tube parameters When running a method, the rack and tube parameters for the rack to be used must be set. This is described in detail in the ÄKTAdesign Optional Configuration User Manual. Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 48: Maintenance

    When your ÄKTAdesign system is cleaned, also clean the capillaries and the accumulator in Frac-950 with distilled water. The instrument should be wiped regularly with a damp cloth. Remember to wipe the DropSync unit photocell as well. Allow the instrument to dry completely before use.

  • Page 49: Trouble-Shooting

    CAUTION! Only spare parts approved or supplied by Amersham Biosciences may be used for maintaining the unit. 5.1 Faults and actions If the suggested actions do not correct the fault, call Amersham Biosciences. Fault Action No tube change 1 Start a flow and start fractionation.
  • Page 50 Trouble-shooting _____________________________________________ Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 51: Reference Information

    Description Instrument Standard mode Prep mode Fraction Collector Frac-950 is an automated fraction collector for use in liquid chromatography as part of an ÄKTAdesign chromatography system. The fractionation base can be selected as time or volume. Collection vessel positions in a rack can be pre-programmed (using standard racks), or occupy any position within the fractionation area (new rack definition).
  • Page 52 New, empty tubes can then be inserted. By using the instruction collecting Ignore_LastTube, fractions can resume. Frac-950 can be set up to handle spillage during tube change in three different ways: • Tube • DropSync •...

  • Page 53: B Technical Specifications

    Prep mode 30 mm rack Accumulator 15–100 ml/min Funnel-to-flask Accumulator 15–100 ml/min 250 ml bottles Accumulator 15–100 ml/min Environment +4 to +40 °C 20–95% relative humidity 84–106 kPa (840–1060 mbar) atmospheric pressure Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 54: Tube Racks

    20 pos. 250 ml bottles 30 pos. 30 mm funnels Rack G , green The following manufacturer’s microplates are tested and approved by Amersham Biosciences for use with this rack type: Greiner low: 655101, 651101, 650101 Greiner high: 780201 Nunc low: 143761, 168055, 156545, 163320 The difference between these microplates is the bottom shape and that a lid is included in some cases.

  • Page 55: Physical Data

    • used in the same state as it was delivered from Amersham Biosciences except for alterations described in the user manual • connected to other CE-labelled Amersham Biosciences instruments or other products as recommended. Safety Standards This product meets the requirement of the Low Voltage Directive (LVD) 73/23/EEC through the harmonized standard EN 61010-1.

  • Page 56: C Accessories And Consumables

    Accessories and consumables Item Quant./ Code no. pack Fraction collector Frac-950 complete with 18-6083-00 Rack A, 18 mm + 30 mm tube rack Rack A, 18 mm + 30 mm tubes, complete 18-6083-11 with bowl, tube support and tube holder...

  • Page 57: Fraction Collector Frac

    ______________________________________ Reference information Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...
  • Page 58 Reference information _____________________________________ Fraction Collector Frac-950 User Manual 18-1139-56 Edition AE...

  • Page 60: Short Instructions

    The instructions assume that optional equipment is installed according to the installation instructions. Frac-950 is controlled from UNICORN. For manual control, use the instructions found in System Control:Manual:Frac 1 For collection of fixed fraction volumes, select the instruction Man_Fractionation and enter the fraction size to start collection.

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