About this manual About this manual Purpose and target group This manual describes the setup, function, operation and maintenance of CO incubators of the types INCO and INCOmed with a chamber volume of 108, 153 and 246 litres. In this manual, the term INCO is used for INCO as well as INCOmed for the purpose of simplification.
Content Content Safety regulations Terms and icons used ......................6 Product safety and dangers ....................7 Requirements of the operating personnel ................8 Responsibility of the owner....................Changes and conversions ....................8 Behaviour in case of malfunctions and irregularities............8 What to do in case of accidents..................
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Content Advanced functions Printer ..........................43 Basic appliance settings (Setup)..................43 Temperature monitoring ....................45 Sterilisation chipcard ......................49 User ID card (optionally available as an accessory) ............50 Calibration ......................... 50 Communication interfaces ....................55 Log memory ........................Maintenance and Servicing Cleaning ..........................
Safety regulations Safety regulations 1.1 Terms and icons used In this manual, certain common terms and icons are used to warn you of dangers or to give you notes that are important in avoiding injury or damage. Observe and follow these notes and regulations to avoid accidents and damage.
Safety regulations 1.2 Product safety and dangers Incubators of the INCO type are technically well-developed, manufactured using high-quality materials and tested for many hours in the factory. They contain the latest technology and comply with recognised technical safety regulations. But there are still dangers involved, even when the appliance is used as intended.
Safety regulations 1.3 Requirements of the operating personnel The incubator may only be operated and maintained by persons who are of legal age, and who have received instructions for the incubator. Personnel who are to be trained, instructed or who are undergoing general training may only be active on the incubator under the con- tinuous supervision of an experienced person.
Safety regulations 1.7 What to do in case of accidents 1. Keep calm. Act resolutely and with consideration. Pay attention to your own safety. 2. Switch off the incubator and close the valves on the gas bottle. 3. Call a doctor. 4.
Design and Function Design and Function 2.1 Design Fig. 2 Design of INCO incubators 1 Controller/control panel (see page 27 ) 7 10 Sliding shelf 2 Set key 11 Door seal 3 Push/turn control 12 Ventilator/inlet opening 4 Connections on the rear of the oven 13 Heating ribs (see page 13 ) 3 14 Adjustable feet...
Design and Function 2.2 Function The air in the incubator is heated up by an all-round heater with a large surface ( Fig. 3 , No. 1). The CO and/or N gas is introduced into the working chamber via a sterile filter. Because CO has a much higher specific weight than air, the gas is let into the working chamber above the interior fan (2).
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Design and Function Digitalised electronic CO control with automatic zero position, NDIR measuring system with self-diagnosis system and acoustic error display, air pressure compensation Language settings (see page 44 ) Alphanumeric text display Integrated week time switch with grup function (e.g. each working day) (see page 31 ) Retracting push-turn control for simple operation of appliance (see page 26 ) Two separate Pt100 temperature sensors DIN Class A in a 4-wire circuit for control and monitoring...
Design and Function 2.4 Material For the outer housing, MEMMERT uses stainless steel (W.St.No. 1.4016), and for the interior, stainless steel (W.St.No. 1.4301), which stands out through its high stability, optimal hygienic properties and corrosion-resistance towards many (but not all!) chemical compounds (caution for example with chlorine compounds).
Design and Function 2.6.1 Electrical connection Observe the country-specific regulations when making connections (e. g. in Germany DIN VDE 0100 with residual current device). This appliance is intended for operation on an electrical power system with a system imped- ance Z at the point of transfer (service line) of a maximum of 0.292 ohm.
RNA and proteins. 2.8 EC Declaration of Conformity You can download the EC declaration of conformity of the appliance online: English: http://www.memmert.com/en/service/downloads/ce-statement/ German: http://www.memmert.com/de/service/downloads/eg-konformitaetserklaerung/ 2.9 Designation (nameplate) The nameplate ( Fig. 5 ) provides information about the appliance model, manufacturer and technical data.
Design and Function 2.10 Technical data Model * See Fig. 6 on page 17 . Chamber width A* [mm] Chamber height B* [mm] Chamber depth C* [mm] Appliance width D* [mm] Appliance height E* (varies due to adjustable feet) [mm] Appliance depth F* (includ- ing door handle) [mm] Chamber volume [litres]...
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Design and Function Model * See Fig. 6 on page 17 . The CO content is determined through an NDIR measur- ing system, controlled constantly by a microprocessor and displayed digitally in percent Adjustment range: 0 to 10 % (for models with CO premium modules 0 to 20 %) Adjustment precision: 0.1 % Variation (time): max.
For incubators with basic fittings: Two (INCO 108) or three (INCO 153 and 246) sliding shelves Gas pressure hose Water tray (one for INCO 108 and 153 incubators, two for INCO 246 incubators) Sterilisation chipcard Additionally, for models with humidity module:...
Delivery, Transport and Setting Up Delivery, Transport and Setting Up 3.1 Safety regulations Warning! You may injure your hands or feet when transporting and installing the incubator. You should wear protective gloves and work shoes. Warning! Because of the weight of the incubator, you could injure yourself if you try to lift it on your own.
Delivery, Transport and Setting Up 3.3.4 Storage after delivery If the incubator is initially to be stored after delivery: Read the storage conditions from page 61 . 3.4 Setup The incubator can be placed either on the ground or on a table (work surface). When doing this, ensure that the appliance is positioned exactly horizontally.
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Delivery, Transport and Setting Up 3.4.1 Base (accessory) The incubator can be placed on a base ( Fig. 8 ). 3.4.2 Stacking frame (accessory) Two appliances of the same model size can be placed on top of one another. To do this, foot alignment provisions must be attached to the lower oven ( Fig.
Putting into Operation Putting into Operation 4.1 Checks 4.1.1 Checking the temperature sensor Especially strong vibrations during transport could result in the temperature sensors being moved in their holders in the working chamber. Check the temperature sensor for its correct positioning and if necessary adjust its position in the holder ( Fig.
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Putting into Operation 1. Fill up the supplied water supply tank (canister) with distilled water and place behind/ next to the incubator. 2. Attach the supplied hose the quick-release connections to the canister and the water sup- ply "H O“ on the rear of the appliance (see Fig. 4 on page 13). 4.2.4 Gas connection Warning! Danger of suffocation: In high concentrations, CO...
Putting into Operation For incubators with O module: Attach the supplied CO pressure hose to the CO gas bottle (pressure reducer) „CO In“ and to the “CO2 In” connection on the „N In“ rear of the incubator with a hose clamp ( Fig.
Operation and control Operation and control 5.1 Operating personnel The incubator may only be operated by persons who are of legal age and have received in- structions for the incubator. Personnel who are to be trained, instructed or who are undergo- ing general training may only be active on the incubator under the continuous supervision of an experienced person.
Operation and control 5.4 Inserting water tray(s) (for appliances with basic fittings) Fill the water tray with distilled water and push into the lowest slot ( Fig. 15 ). INCO Number Filling Amount model level in of water water cm for in ltr.
Operation and control 5.7 Basic operation The desired parameters are entered on the operating panel of the controller on the front of the appliance ( Fig. 18 ). Basic settings, as well as those for time and printing, can also be made here.
Operation and control The control returns automatically to the main menu if the push-turn key or set key is not operated for approx. 30 seconds. Setting the temperature (Quick adjustment): 1. Hold down the SET key and set the desired temperature setpoint with the push-turn control.
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Operation and control 5.10.1 Normal mode The appliance runs in this operating mode in permanent operation. The desired setpoints for operating the chamber can be selected. The settings have an immediate effect on the func- tions of the appliance. 1. Load incubator (see page 25 ). 2.
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Operation and control 5.10.2 Settings example normal mode With a 5% CO content, a 3% oxygen content and a humidity of 96%, the appliance should heat up to 37°C. The monitoring function should respond at 38.5 °C ( Fig. 20 ). Monitoring temperature Time Time...
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Operation and control 4. Setting the humidity setpoint: High levels of air humidity in the interior can only be achieved without condensation if the interior is thoroughly heated. Please set the humidity only when the temperature in the interior has been stable for at least 30 minutes.
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Operation and control Weekday y Adjustment range: Monday to Sunday Day groups Adjustment range: Working days Mo-Fr Weekend Sat-Sun No switch on time: ---- Appliance not switched on on this day Switch on time (on) Adjustment range: 00:00 to 23:59 hours Switch off time (off) One minute beyond the switch on time up to 24:00...
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Operation and control 5.10.4 Settings example week time switch From Mo-Fr (workdays group), the appliance should switch on at 9.30 and switch off at 19.00. In addition it should work on Saturday from 10.00 to 14.00 ( Fig. 21 ). 24:00h 12:00h 01:00h...
Operation and control 4. Switch on Sa at 10:00 With the push-turn control, select "Sat on". Hold down the SET key and set the desired switch-on time with the push-turn switch to 10:00. 5. Switch off Sa at 14:00 With the push-turn control, select "Sat off". Hold down the SET key and set the desired switch-off time with the push-turn switch to 14:00.
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Operation and control After the setpoint temperature has been reached, IN 1 IN 2 the CO concentration is displayed in %, depending on the setting. indicates that gas bottle 1 IN 1 is active. Is displayed if the CO concentration exceeds the defined setpoint by at least 1 % for more than 3 minutes.
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1 is used up, you connect the opened bottle to IN1 and the newly filled bottle as a reserve bottle to IN2. The hose connection system used by Memmert shuts off automatically if a connection hose is pulled off. You should still always close the stop valve on the gas bottle if a bottle is empty or not connected.
Operation and control 5.12 Ending operation 1. Switch off appliance. To do this, press the main switch on the operating panel so that it clicks into place in the appliance (see Fig. 22). 2. Close the valve(s) of the gas bottle(s). 3.
Warning messages and malfunctions Warning messages and malfunctions 6.1 Warning messages An intermittent tone is also set off by the warning messages. This can be temporarily switched off by pressing the SET key. Remedy: Close the door DOOR OPEN if the door of appliance is opened for longer than 3 minutes.
Do not try and rectify the error yourself, but contact an authorised customer service point for MEMMERT appliances or Contact the customer services department directly of the company MEMMERT (see page 2 ). In case of enquiries, please always specify the model and appliance number on the nameplate...
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Warning messages and malfunctions Error Possible causes Remedy Power supply Check power supply and fuse/safety display although the interrupted switch. incubator is switched Appliance fuse or miniature fuse or controller faulty Contact the customer service and read the service manual. Appliance error Mainboard faulty Appliance cannot be...
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Warning messages and malfunctions Error Possible causes Remedy Checksum error (error The error can be rectified just by the Error message CONF when saving setpoint controller after a setpoint parameter (is displayed for only 10 values) has again been saved. If the error sec.
Warning messages and malfunctions 6.3 Power failure In case of a power failure, the incubator operates as follows: In normal and week time switch operating modes After the power supply has been restored, operation is continued with the parameters set. The time and the duration of the power failure is documented in the log memory.
Advanced functions Advanced functions 7.1 Printer (only for models with communication or premium modules) Incubators with a communication or premium module are equipped with a parallel printer port, as used in computers. Standard PCL3 compatible inkjet printers with a parallel port (e.g. HP DeskJet 5550 or HP DeskJet 9xx) can be connected to the printer interface on the rear of the appliance (see page ).
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Advanced functions By turning the push-turn control and holding down the SET key, the following parameters can be selected and altered one after another, as described in Chapter “ Basic operation “ on page 27 : Clock time in 24 hr. format Conversion to summer time is not automatic, but must be done manually.
Advanced functions Oxygen calibration (Cal 0 (only for models with O module, see page 54 ) C A L o o f f Leave Setup p Save all settings and leave the SETUP mode. The realtime clock, which is set in the SETUP, contains the date and clock time. It is used for logging purposes in accordance with GLP.
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Advanced functions Error rectification after the TB has been triggered: 1. Switch off appliance and leave to cool down 2. Contact the customer service and have the error rectified (e.g. replace temperature sen- sor). The appliance is only operational again after it has cooled down and the error has been elimi- nated.
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Advanced functions Setting: g Overtemperature limit: up to max. 10 °C above nominal temperature °C (for details of the nominal temperature, see nameplate) Setting: 1. Select the icon with the push-turn control. AUTO 2. Hold down the SET key and set the desired temperature limit with the push-turn control (e.g.
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Advanced functions 7.3.3 Automatic temperature monitor (ASF) ASF is a monitoring device that automatically follows the set temperature setpoint within an adjustable tolerance band ( Fig. 25 ). The ASF is activated – if switched on – automatically if the actual temperature value reaches 50 % of the set tolerance band of the setpoint (in the example: 37 °C –...
Advanced functions 7.4 Sterilisation chipcard For decontamination, the incubator is equipped with a STERICard as standard ( Fig. 26). The STERICard starts an automatic and predetermined sterilisa- tion sequence. Warning! The incubator becomes Sterilisation Parameter: very hot inside during 160 °C / 4:00 h Sterilisation the sterilisation proce- Process Control...
Advanced functions 7.5 User ID card (optionally available as an accessory) The device number of the incubator and a unique user number are stored in encrypted form on the user ID card ( Fig. 27 ). The user ID card therefore works only in the appliance with the corre- sponding serial number.
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Advanced functions Setting: g 1. Set compensation temperature in the SETUP (see page 44) and set accompanying com- pensation correction value to 0.0 °C. 2. With a reference instrument, measure the deviation in the stationary state in the selected compensation temperature. 3.
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Advanced functions 7.6.2 Humidity calibration The incubator can be calibrated for the individual customer by means of two balance points at 20 and at 90 % relative humidity. For each selected balance point a positive or negative compensation correction value can be set between –5 % and +5 %. rh 90 -2,0 % rh 20...
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Advanced functions 4. The reference instrument should display 90.0% rh after the calibration procedure. With RH 20 a further comparison can be programmed at 20 % relative humidity. If all compensation correction values are set to 0.0 % rh, the factory calibration settings are restored.
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Advanced functions Example: CO deviation in chamber load at 10 % should be corrected: balance point in the SETUP to CO2 10 and set the accompanying compensation 1. Set the CO correction value to 0.0 %: STERI DEFRO IN 1 IN 2 IN 1 IN 2...
Advanced functions 7.7 Communication interfaces (only for models with communication or premium modules) If the incubator is equipped with the communication or premium module, its log data can be read out with a computer/laptop. For this purpose, the incubator has corresponding commu- nication interfaces on the rear of the appliance (see page 13 ).
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Advanced functions Fig. 32 Connecting incubators via the RS 232-C communication interface 7.7.2 Bus interface RS 485 If requested on the corresponding order, the oven can be fitted in the factory with a RS-485 interface instead of a RS-232-C interface. This enables the networking of several ovens (up to 16) with one computer via a shared two-wire cable ( Fig.
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Advanced functions Interface converter RS232 RS485 Fig. 34 Connecting incubators via the RS485 bus interface 7.7.3 USB interface If several chambers are to be connected to a computer via USB interface, an appropriate interface on the computer and a separate cable are required for each chamber. The maximum cable length is 5 m.
Advanced functions For identification purposes, each appliance connected must have its own unique IP address. Each chamber is delivered by default with the IP address 192.168.100.100. The program “XTADMIN”, which can be found on the CD-ROM provided, can be used to change the IP ad- dress.
Maintenance and Servicing Maintenance and Servicing 8.1 Cleaning Warning! Danger of injury. Before any cleaning work, pull out the mains plug. Regular cleaning of the easy-to-clean chamber interior prevents build up of material remains, which over time could impair the appearance and functionality of the stainless steel chamber.
Maintenance and Servicing 8.3 Adjusting door A well-closing door is indispensable for incubators. On Memmert appliances, the tight closing of the door is optimally guaranteed by a chamber seal and a door seal (see also page 10 ). In permanent operation, it is possible that the flexible seal material will begin to sag.
Storage and Disposal Storage and Disposal 9.1 Storage The incubator may only be used under the following conditions. Dry and in an enclosed, dust-free room Frost-free Disconnected from the power supply and gas supply Undo gas bottle connections and close valves of gas bottles. Gas bottles may be stored in closed rooms if these are sufficiently well ventilated.
Index Index Door 25 Door seals 59 Accessories 18 Main switch 26 Accidents 9 Maintenance 59 Acoustic signal 44 Malfunctions 8, 38 EC Declaration of Conform- Additional modules 12 Manufacturer 2 ity 15 Adjusting door 60 Material 13 Electrical connection 14 Advanced functions 43 Memory 58 Electrical equipment 13...
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Index Safety regulations 6 Technical data 16 Unpacking 19 Service 60 Temperature calibration 50, USB interface 57 Servicing 59, 60 User ID card 50 Setting parameters 27 Temperature comparison 50 Settings example normal Temperature deviation 51 Warning messages 49 mode 30 Temperature limiter (TB) 45 Water connection 22 Settings example week time...
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