3D Cultures 3D Bio-Printer User Manual

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3D BIO-PRINTER

3D Cultures

USER MANUAL Version 2.0

 Product pictures are for reference only.

 Color(s), size, parts, and interface may vary.

 Preheating is not recommend with the use of cells.

 This manual and specifications described herein are subject to change without notice.

 All trademarks are the sole property of their respective companies.

 This product does not provide a User Interface but reference many that are helpful.

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Summary of Contents for 3D Cultures 3D Bio-Printer

Page 1  This manual and specifications described herein are subject to change without notice.  All trademarks are the sole property of their respective companies.  This product does not provide a User Interface but reference many that are helpful.  Copyright © 2017 3D Cultures. All Rights Reserves.
Page 2: Table Of Contents
TABLE OF CONTENTS SAFETY WARNINGS & PRECAUTIONS ....................2 OVERVIEW ..............................3 PACKAGE CONTENTS ..........................3 FEATURES ..............................4 CUSTOMER SERVICE & TECHNICAL SUPPORT .................. 4 USING THE INTERACTIVE SCREEN SYSTEM ..................4 SOFTWARE INSTALLATION........................4 Installing Cura for Windows........................4 Installing Repetier-Host ...........................
Page 3: Safety Warnings & Precautions
SAFETY WARNINGS & PRECAUTIONS » This device is HOT! Do not touch hot surfaces. » The extruder holder can be very HOT! Do not touch when hot. » The build platform can be very HOT! Do not touch when hot. »...
Page 4: Overview
OVERVIEW Thank you for purchasing this 3D Bio-Printer by 3D Cultures. At 3D Cultures, our aim is to use existing technologies and make by printing affordable. There are tons of new, great, and open source technology today that can aim in the development of 3D Bioprinting. This device/printer is built on a open source platform which requires some skills from the end-user.
Page 5: Features
 Includes a 256MB microSD card with Cura, Repetier-Host, and Nozzle Setup file. CUSTOMER SERVICE & TECHNICAL SUPPORT 3D Cultures’ Support team is always available to help. If you need any assistance please contact us via email 3dcultures@gmail.com USING THE INTERACTIVE SCREEN SYSTEM This printer has an interactive screen to help with all your printing needs.
Page 6 3. Ensure that all boxes are checked, then click install to continue. 4. Once the files have been extracted and Cura installation is complete, the driver installation wizard will launch. Click next to continue. 5. Click Finish to complete the driver installation. P a g e...
Page 7 6. Click next to continue. 7. Ensure that the nox nest to the Start Cura 15.04.2 option is checked, then click Finish to complete the installation and launch the program. 8. Wait for Cura to finish launching. If this is the first time you have run Cura on this machine, Cura will automatically launch the First time run wizard.
Page 8 9. Click the button next to other option, then click Next button to continue. 10. Select the Custom…. Option, then click Next to continue. P a g e...
Page 9 11. Change the settings to match those shown in the image above, then click Finish to continue. 12. The program will finish launching and leave you on the main screen. Select the Basic tab, then change the settings to match those in the accompanying image for PLA filament. (PLA is generic since these interface are not set for bio-materials.
Page 10 Since there are no set standards in bioprinting, the selection of the features/settings below is up to the user. Take notes of what works for your printing needs. Note that it will require a few trials to get it right. It is recommended to test your printing before printing the actual experimental sample. 13.
Page 11: Installing Repetier-Host
Installing Repetier-Host You can also install Repetier-Host from the file on the included microSD card from an internet download. The screenshots below shows the proper settings to use for this bio-printer in Repertier-Host. 10 | P a g e...
Page 12: Printer & Nozzle Setup
PRINTER & NOZZLE SETUP Perform the following steps to prepare the printer for use. Out of Box Setup 1. Remove the printer from its packaging and place it on a flat, stable surface with plenty of ventilation and a nearby AC power outlet. 2.
Page 13: Zeroing The Nozzle
3. Ensure that the power switch on the rear panel is in the OFF position (“O” side depressed). Plug the C13 end of the included AC Power Cable into the C14 panel connector on the AC Power Adapter, then plug the other end into a nearby AC power outlet. Press the “I” side of the power switch to power the printer ON.
Page 14 5. Insert the syringe with the tip. 6. Position the nozzle (with the syringe) where the tip barely touches the base of the petri dish. 7. Tighten both hex screw with the hex tool. 13 | P a g e...
Page 15 8. Using the interactive screen, move the nozzle up. 9. The tip of your nozzle is now at the zero Z position and is ready to print. 10. Note: Ensure the nozzle is at zero position for best printing. If printing into a petri dish, ensure your code takes into consideration the dimensions/parameters of the dish.
Page 16: Regulatory Compliance
REGULATORY COMPLIANCE NOTICE FOR FCC This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.