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Qiagen RT2 First Strand Kit Manual

Rt2 profiler pcr array (rotor-gene format) human obesity

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RT² Profiler PCR Array (Rotor-Gene
Human Obesity
Cat. no. 330231 PAHS-017ZR
For pathway expression analysis
Format
RT² Profiler PCR Array,
Format R
Description
The Human Obesity RT² Profiler PCR Array profiles the expression of 84 genes related to
obesity. The control of a relative stable body weight depends on a complex interaction
among the hormone axes in the peripheral nervous system and neurotransmitter signals in
the central nervous system that play a crucial role in maintaining a balanced energy
metabolism. This PCR Array includes obesity-related genes that are directly involved in the
regulation of energy intake and expenditure. The genes encode for orexigenic peptides,
hormones, and receptors; anorectic peptides, hormones, receptors; and central and
peripheral signaling molecules involved in energy expenditure. Using real-time PCR, you
can easily and reliably analyze expression of a focused panel of genes related to Obesity
with this array.
For further details, consult the
Shipping and storage
RT² Profiler PCR Arrays in the Rotor-Gene format are shipped at ambient temperature, on
dry ice, or blue ice packs depending on destination and accompanying products.
For long term storage, keep plates at –20°C.
: Ensure that you have the correct RT² Profiler PCR Array format for your real-time
Note
cycler (see table above).
: Open the package and store the products appropriately immediately on receipt.
Note
For use with the following real-time cyclers
Rotor-Gene Q, other Rotor-Gene cyclers
RT² Profiler PCR Array Handbook
Format)
®
.
Sample & Assay Technologies

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Summary of Contents for Qiagen RT2 First Strand Kit

  • Page 1 RT² Profiler PCR Array (Rotor-Gene Format) ® Human Obesity Cat. no. 330231 PAHS-017ZR For pathway expression analysis Format For use with the following real-time cyclers RT² Profiler PCR Array, Rotor-Gene Q, other Rotor-Gene cyclers Format R Description The Human Obesity RT² Profiler PCR Array profiles the expression of 84 genes related to obesity.
  • Page 2 Array layout The 96 real-time assays in the Rotor-Gene format are located in wells 1–96 of the Rotor-Disc™ (plate A1–A12=Rotor-Disc 1–12, plate B1–B12=Rotor-Disc 13–24, etc.). To maintain data analysis compatibility, wells 97–100 do not contain real-time assays but will contain master mix to account for weight balance.
  • Page 3 Position UniGene GenBank Symbol Description Hs.471619 NM_006056 NMUR1 Neuromedin U receptor 1 Hs.1832 NM_000905 Neuropeptide Y Hs.519057 NM_000909 NPY1R Neuropeptide Y receptor Y1 Hs.122926 NM_000176 NR3C1 Nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) Hs.494312 NM_006180 NTRK2 Neurotrophic tyrosine kinase, receptor, type 2 Hs.80962 NM_006183 Neurotensin...
  • Page 4 For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen. com or can be requested from QIAGEN Technical Services or your local distributor.

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