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Covaris S220 User Manual

S220 series
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For research use only
Instrument and software developed for
high performance sample preparation
using adaptive focused acoustic energy
Product Name:
Model Numbers:
Software:
S220 Series
USER MANUAL
S220-Series
S220, S220x, S220R
SonoLab 7

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Summary of Contents for Covaris S220

  • Page 1 For research use only S220 Series USER MANUAL Instrument and software developed for high performance sample preparation using adaptive focused acoustic energy Product Name: S220-Series Model Numbers: S220, S220x, S220R Software: SonoLab 7...
  • Page 2 Registered names and trademarks used in this document, even when not specifically marked as such, are not to be considered unprotected by law. WCS and AFA are either registered trademarks or trademarks of Covaris, Inc. in the United States and/or other countries.
  • Page 3 Establish a standard of operation and periodically test equipment, as described in Maintenance (see section 6.0) of this manual. Loading third party software onto the computer may interfere with system operation. Please consult with Covaris. S220(x) User Manual Page 3...
  • Page 4 Covaris personnel. If Covaris personnel are required to perform on-site repair, all travel related costs are paid by the customer. A limited warranty as specified may apply to certain components of the equipment.

  • Page 5: Table Of Contents

    Cleaning the System ........................31 TROUBLESHOOTING ........................32 Appendix A: Suggested S220 Series IQ / OQ / PQ ..................34 Appendix B: S Series Site or Transport Preparation ................... 40 Appendix C: Installing SonoLab Software, Firmware and Instrument Drivers ..........41 Appendix D: Recirculating Chiller ........................

  • Page 6: Introduction

    This manual contains operation and maintenance instructions for the Covaris S220 Series Focused- ultrasonicators (S220, S220x or S220R). In this document, “S220” generally refers to any of these S220 Series of instruments. This document contains information essential to the proper use and care of the equipment.

  • Page 7: Installation And Setup

    Sample Tube Holders (may vary according to application) o S220 Water Tank o S220 Main Unit Please note that the SonoLab software installer, if required, is available from the Covaris website or by contacting Covaris technical support. The S220 Focused-ultrasonicator...

  • Page 8: S220 Series Installation

    S220 Series Installation 2.2.1 Unpack the S220 and place it on a table or bench, with space to accommodate the instrument and the laptop. The recirculating chiller may be placed on the floor to save bench space. 2.2.2 Please refer to the rear panel of the S220 and identify the following features:...
  • Page 9 Please refer to the chiller’s user manual. 2.2.9 Unpack the Water Tank. 2.2.10 At the front of the S220, lift the Acoustic Assembly, slide the Tank into the frame as shown, and lower the Acoustic Assembly. The WATER LEVEL label should face forward.

  • Page 10: Getting Started

    ON, this switch will illuminate and the ventilation fans in the rear panel will run. Also start the external chiller, programming its set point to a few degrees below the desired S220 bath temperature. Be sure to employ a 20% glycol solution inside the chiller to prevent freezing. Do not use undiluted glycol solution.

  • Page 11: The Sonolab Run Screen - Running A Method

    Following the splash screen, the SonoLab Run Screen will appear: If the S220 is powered ON and connected to the laptop via the USB cable, SonoLab will connect to the instrument, display its model type (e.g. “S220”) at the top center of the screen, and display the message, “Connected to Instrument”...
  • Page 12 Before running a method, load a sample tube into a holder, open the Safety Cover and place the holder into the S220. Then, close the Safety Cover.  The S220 does not control its own water temperature. This must be controlled by adjusting the set point of the external chiller. ...
  • Page 13 Average acoustic power (in Watts) and water bath temperature will be displayed numerically and graphically at the lower right corner of the Run Screen, along with a progress bar for the method being run: Note that the average Power values will approximate (Peak Power x Duty Factor). S220(x) User Manual Page 13...

  • Page 14: System Shutdown

    A new sample tube may then be inserted into the holder and the process repeated. If there is additional sample processing to be done, the S220 system and SonoLab may be left ON and operating. If the system will be idle for an extended period of time (e.g. overnight or a weekend), then please refer to the next section on System Shutdown.

  • Page 15: Temperature Control

    Each protocol published by Covaris specifies a desired bath temperature. The S220 does not control its own water temperature. This must be controlled by adjusting the set point of an external chiller. This chiller pumps fluid through hoses connected to the rear panel of the unit (see Setup) and then through stainless steel tubes immersed in the water bath.

  • Page 16: Sonolab™ Application Software

    SonoLab™ APPLICATION SOFTWARE SonoLab is an application used to control a Covaris AFA (Adaptive Focused Acoustics) instrument for processing a sample. It is designed to create, edit, and run a sample processing program called a method. It has provisions to run, pause or stop a method and view execution histories which contain a record of key instrument parameter values during a run.

  • Page 17: Create And Edit Methods

    If creating a new method, in the Basic Settings tab, select the “Add Step” to create a 4.2.2 treatment. Basic Settings Add Step Create Method / Edit Method Screen The Add Method Step window will appear. The Step Types available in this screen are Treatment, Delay, Prompt and Repeat. S220(x) User Manual Page 17...
  • Page 18 15%, the Average Incident Power will be approximately 15 Watts, but will vary with sample tube type and water level and temperature. In the S220 instrument, the maximum average power is 100 Watts. In the S220x instrument, the maximum average power is 250 Watts, allowing application of greater average power.
  • Page 19 Add a Prompt Step By choosing a Repeat step, the user can create a group and a number of repeat iterations. Steps may then be moved into this group (see Available Functions below). Add a Repeat Step S220(x) User Manual Page 19...
  • Page 20 Screen. Values may be set as low as 0.5°C and as high as 55.0°C. 4.2.5 If User Authentication is enabled, a method may be locked against editing by other users. See “Operation with User Authentication” for more details. S220(x) User Manual Page 20...

  • Page 21: Temperature Tab

    Resume Values are normally 0.5°C inside the Limit Values, but can be edited to create a wider margin Changes in a method’s attribute may be saved by clicking Save, Save As, or discarded by clicking Cancel. S220(x) User Manual Page 21...

  • Page 22: Saving Methods In Folders

    Folder dialog will appear. Enter Folder Name, then click OK. The new folder will appear under Methods as shown below. Additional subfolders may be created by highlighting Methods and clicking New Folder, or by highlighting any subfolder and clicking New Folder. S220(x) User Manual Page 22...

  • Page 23: History Tab

    4.6.1 Communications SonoLab will automatically connect to the S220 if the unit is powered on and linked by a USB cable. No action by the user is required. The typical connection method will be “USB Port”. The Communications tab allows the user to manually connect with the S220 if automatic connection fails.
  • Page 24 By default, authentication is disabled and the Log In button in the upper right corner of the SonoLab screen is disabled. This is the typical configuration for the S220 – all users have equal access to methods and settings.
  • Page 25 Authentication tab. To secure the system, make note of the admin password, leave User Authentication enabled, and log out of the admin account by clicking on Log Out (upper right corner of the SonoLab screen: S220(x) User Manual Page 25...
  • Page 26 Click Yes and log into Task Scheduler Login under your Windows user name.  SonoLab will restart and, if requested, Windows Task Scheduler will delete files at 7:00 pm (19:00) according to the configuration saved under File Settings. S220(x) User Manual Page 26...
  • Page 27  SonoLab will restart. Windows Task Scheduler will turn the degas pump on or off at the requested time. The S220 unit must be switched ON and connected to the laptop for these tasks to occur. Users may also manually run the degas pump for 10 seconds by clicking “Prime Pump”.

  • Page 28: Maintenance Screen

    An example of one of the informational screens is shown below: Additionally, a SonoLab Administrator may employ the Firmware Update tab if SonoLab posts a notice that the firmware in the S220 is out of date, as described in the next section. 4.7.1...

  • Page 29: About Tab

    About Tab The About Tab provides the user with basic information about the instrument such as serial number, software version, and board serial numbers. Contact information for Covaris is also displayed. Clicking Help provides detailed Applications and Service information. Clicking on Service Information creates a file, Status.ZIP, which captures status files from the instrument.

  • Page 30: System Specifications

    Bench-top; high intensity acoustic transducer, temperature monitoring Treatment System: device, circulation pump, water bath with safety enclosure 500 Watts Peak Incident Power, 100 Watts Average Power (S220), 250 Treatment Power: Watts Average Power (S220x,R) 8” W x 21” D x 13” H (20cm x 53cm x 33cm)

  • Page 31: Maintenance

    When the pump is running, verify that there are small bubbles coming from the end of the outlet nozzle. If you suspect that the system has become clogged, please contact Covaris for assistance. There is a small inlet tube that can be removed and cleaned.

  • Page 32: Troubleshooting

    20% water-glycol mix) is circulating through the S220 chiller coils. Program the set point of the chiller to a lower temperature and verify that the S220 coils get cold. Use a separate thermometer to verify the true temperature of the bath.
  • Page 33 In SonoLab, go to the About tab and click Service Information. This will create a ZIP file on the desktop that can be emailed to Covaris or your local representative. Contact Covaris or your local representative and provide the information listed above.

  • Page 34: Appendix A: Suggested S220 Series Iq / Oq / Pq

    Appendix A: Suggested S220 Series IQ / OQ / PQ All Covaris instruments are rigorously tested at the Manufacturing Facility prior to shipment. This is done to ensure that your Covaris device is of the highest quality and meets your needs for consistent, efficient, and reliable Sample Preparation.
  • Page 35 S220 SERIES - INSTALLATION QUALIFICATION (IQ) DATE: INSTRUMENT S/N: COMPLETED BY: Action / Requirements Ref. Section Requirement Met? Section 1: Shipping Contents and Instrument Condition Instrument is successfully unpacked and all items noted in Packing List are present. Denote any missing items here: _______________________________ There is no damage evident to the instrument or accessories.
  • Page 36 Verify computer is connected to instrument via USB cable provided. Power on the computer and log in (as required). Note: If Laptop is not provided by Covaris, refer to SonoLab / Driver installation sections of User Manual. Double-click the ‘SonoLab’ shortcut on the computer desktop; verify the application starts and SonoLab main screen successfully displays.
  • Page 37 S220 SERIES – OPERATIONAL QUALIFICATION (OQ) (2 of 2) DATE: INSTRUMENT S/N: COMPLETED BY: Turn Degas Pump back ON with SonoLab ‘switch’ and click the Override check box. Ensure the ‘Run’ button changes color from Gray to Green. Click (Run) on the SonoLab Main Screen to start the recently created Method.
  • Page 38  Configure Chiller with appropriate Set Point to achieve Water 40-60 Watts) Bath Temperature of 14-17°C.  Obtain a THQ 13 (Covaris # 500011) holder and 13 x 65 mm tube (Covaris #520010). Note: If this tube and/or holder are not available, please contact Covaris Customer Service for an alternate test method using existing materials.
  • Page 39 S220 SERIES – PERFORMANCE QUALIFICIATION (PQ) (2 of 2) DATE: INSTRUMENT S/N: COMPLETED BY: Degas Performance Test – OPTIONAL (Calibrated O2 Meter Required)  Fill Water Bath with fresh water (10-15 on ‘Fill’ side of Water Level Label). Use a calibrated Dissolved Oxygen Meter (i.e.

  • Page 40: Appendix B: S Series Site Or Transport Preparation

    The area required for the instrument (see attached drawings) is approximately 8” wide by 21” deep by 13” high (20cm x 53cm x 33cm). Space adjacent to the S220 is required for the laptop computer, and additional space is required for the recirculating chiller on the bench adjacent to the S220, or preferably under the bench.

  • Page 41: Appendix C: Installing Sonolab Software, Firmware And Instrument Drivers

    When installation is complete, close the installer. A “Sonolab 7” shortcut should appear on the computer desktop. 5. With the S220 powered ON, double click on the Sonolab 7 shortcut. SonoLab will open its splash screen, as described in Section 3 of this manual.
  • Page 42 Automatic driver installation: If any S220 has ever been connected to the computer, then a copy of the appropriate drivers will be available to Windows locally, and Windows will automatically install these. If Windows does not find a local copy of the necessary drivers, it will automatically search Windows Update (assuming internet access is available via wireless link or Ethernet cable and Windows is configured for automatic updates).
  • Page 43 Under Other devices, right click first device RF Controller Board and select Update Driver Software: When the driver software update dialog opens, select the option to Browse my computer for driver software: Point the file open dialog to “C:\Program Files\Covaris\Driver” and verify that “Include subfolders” is checked: S220(x) User Manual Page 43...
  • Page 44 “Install the driver software anyway”: Windows will install the driver and post the following dialog box with a message, “Windows has successfully updated your driver software”. Click the Close button. The Device Manager screen will refresh. S220(x) User Manual Page 44...
  • Page 45 Return to Other devices and repeat this process in order (top to bottom), for the 3 remaining entries, until all Covaris device entries have been assigned a COM number under Ports (COM & LPT). The ‘Device Manage’ window should now show: Note that the ‘COM’...

  • Page 46: Appendix D: Recirculating Chiller

    Quick-Disconnect Coupling Plug, ¼ inch, for 3/8 inch interior diameter tube ID. This end of each hose is designed to connect to the chiller connections on the rear panel of the S220. The other end is left open to allow connection to a variety of chillers. These connections are typically made with barbed fittings equipped with a barbed adapter able to connect to the chiller.
  • Page 47 S220 are cold. Fill the S220 bath with DI water, start SonoLab, and allow the chiller and degas pump to operate for at least 30 minutes. Verify that the Degas status has turned green, and that the bath temperature has reached (or is approaching) a few degrees above the chiller’s set point.

This manual is also suitable for:

S220rS220x

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