Summary of Contents for Warner Instruments PLI-10
- Page 2 The PLI-10 Pico-liter Injector is designed to provide controlled delivery of picoliter to nanoliter volumes of solution through a glass micropipette. The PLI-10 is ideal for applications such as neuronal tract-tracing, delivery of pharmacological agents in vitro, and Xenopus oocyte injection.
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Page 3: Table Of Contents
APPENDIX..............................14 Specifications............................14 Ordering Information .......................... 14 Warranty and Service .......................... 15 Warranty............................. 15 Service ..............................15 Certifications............................16 Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278 Rev 090714... -
Page 4: Nomenclature
Finally, references to individual control settings are specified in italic type. Special comments and warnings are presented in highlighted text. Any other formatting should be apparent from context. Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278 Rev 090714... -
Page 5: General
Xenopus oocyte injection. These ‘large volume’ injections (10 pl to greater than 100 pl) are common for many applications that do not require multiple pressure settings. As such, the PLI-10 is an effective, low-cost alternative to our more elaborate pressure ejection systems (e.g., the PLI-90 and PLI- 100). -
Page 6: Rear Panel
M. PRESSURE INPUT (P ) connector: This connector is the input for the compressed gas. 75 PSI is the recommended input pressure; maximum input pressure is 105 PSI. Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278... - Page 7 Pushing the foot switch triggers an injection for the preset time and pressure values displayed on the instrument front panel. Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278...
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Page 8: Setup And Operation
SETUP AND OPERATION The PLI-10 is provided with input and output hoses for gas handling and a foot switch for triggering injections. Additional requirements include an acrylic pipette holder (see ordering information below) and glass micropipettes. A regulated air/gas supply and a micromanipulator to hold the injection pipette are needed to create a complete microinjection system. -
Page 9: Additional Information
Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278... -
Page 10: Required Supplies
Different strategies have been used to suitably fix the cell in position for successful intracellular injection: Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278... -
Page 11: General Considerations
Extracellular delivery is nearly always “large”. Intracellular injection is often of “small” volumes (but not for frog oocytes). Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278 Rev 090714... -
Page 12: Injection Pressure And Time Pulse
To prevent this from happening, check each connector for tightness by turning clockwise. This will ensure needle valve depression. Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278... -
Page 13: Volume Calibration Chart
7.18 8976.00 8.98 10771.20 10.77 75.74 0.08 757.35 0.76 1514.70 1.51 2272.05 2.27 3029.40 3.03 3786.75 3.79 4544.10 4.54 Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278 Rev 090714... -
Page 14: Appendix
Acrylic pipette holder for 1.5mm pipettes 64-1629 AO162.0 Acrylic pipette holder for 2.0mm pipettes 65-0008 PLI-IHN Gas Input Hose 65-0010 PLI-OHN Gas Output Hose Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278 Rev 090714... -
Page 15: Warranty And Service
Warranty and Service Warranty The PLI-10 Pico-liter Injector is covered by our Warranty to be free from defects in materials and workmanship for a period of one year from the date of shipment. If a failure occurs within this period, we will either repair or replace the faulty component(s). -
Page 16: Certifications
Directive(s) and Standard(s). Place: Hamden, Connecticut Signature: Full Name: Ralph Abate Position: General Manager Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278 Rev 090714... -
Page 17: Fax (203) 776-1278 Rev
Directive(s) and Standard(s). Place: Hamden, Connecticut USA Signature: Full Name: Ralph Abate Position: General Manager Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278 Rev 090714... - Page 18 Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA Warner Instruments • 1125 Dixwell Avenue, Hamden, CT 06514 USA (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278 (203) 776-0664 • (800) 599-4203 • Fax (203) 776-1278 Rev 090714...