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EZ Read 800 Plus User Manual

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EZ Read 800 Plus Microplate Reader
User Manual
Biochrom Ltd
22 Cambridge Science Park
Cambridge
UK
CB4 0FJ
Tel.: +44/1223 423723
1

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  • Page 1 EZ Read 800 Plus Microplate Reader User Manual Biochrom Ltd 22 Cambridge Science Park Cambridge CB4 0FJ Tel.: +44/1223 423723...

  • Page 2: Table Of Contents

    Table of Contents 1. INTRODUCTION AND INTENDED USE ..................4 1.1 W ’ ..................4 HAT TO EXPECT FROM THE USER S MANUAL 1.2 I ........................... 4 NTRODUCTION 2. INSTRUMENT USE ........................5 3. INSTRUMENT CONNECTION AND SOFTWARE INSTALLATION AND USE ......... 6 3.1 I ........................
  • Page 3 6.3 I ........................41 NSTRUMENT CLEANING 6.4 A ......................41 DDITIONAL MAINTENANCE TIPS 7. WARRANTY AND RETURN TO BASE ..................41 7.1 W ....................41 ARRANTY TERMS AND CONDITIONS 7.2 R ...................... 42 ETURNS TERMS AND CONDITIONS 7.3 R ........................43 ETURNS PROCEDURE 8.

  • Page 4: Introduction And Intended Use

    1 Introduction and Intended Use 1.1 What to expect from the user’s manual  Instrument use  Software installation  Quick start guides for operational use (to perform a quick measurement, kinetics and multi-wavelength measurements).  A technical explanation of how the instrument operates ...

  • Page 5: Instrument Use

    400-750 nm in optically clear 96-well plates with a standard SBS/ANSI footprint. Only trained laboratory personnel should operate the Biochrom EZ Read 800 Plus Microplate Reader. The Biochrom EZ Read 800 Plus Microplate Reader is intended for general laboratory and research use only.

  • Page 6: Instrument Connection And Software Installation And Use

    3.2 Software installation Galapagos is supplied with all EZ Read 800 Plus instruments. The software can be used to control the microplate reader to measure all endpoint, kinetic and multi-wavelength assays. 1. To connect the instrument to a PC: Connect to the PC via the supplied USB cable.

  • Page 7: Quick Measurement

    3. Click ‘Quick Measurement’: 4. The microplate reader is now ready for use. 3.3 Quick Measurement: Performing a Quick Measurement on Galapagos: 1. Click the ‘Quick Measurement’ icon on the main menu screen: 2. Select the appropriate measurement mode (Single, Dual or Multi-wavelength): b.
  • Page 8 3. Users can also select to shake the microplate. Users can select shaking to be a single event or in timed cycles. 4. Select the amplitude (degree of linear shaking). 5. Select the speed for linear shaking. 6. Users can select the duration of shaking.
  • Page 9 7. Click ‘Start’ to begin acquisition. 8. Raw absorbance values for dual wavelengths measurements are displayed in the wells. Details of corresponding wavelengths can be displayed when clicking on the individual well.
  • Page 10 9. When users click onto a well, it is highlighted (dashed lines). In regards to dual wavelength measurements, three absorbance values are displayed. The bold value refers to the absorbance measurement after wavelength subtraction of the absorbance at the measurement wavelength from absorbance at the reference wavelength.
  • Page 11 10. Results can be saved to ‘Database’. A database is useful for saving, sharing and extracting data via communication with an external database system such as LIMS. Users can save files and templates onto the database, ‘File’ (save to own personal file), ‘Save...

  • Page 12: Inetic Measurement

    3.4 Kinetic Measurements Performing a kinetic measurement on Galapagos: Kinetic measurements refer to recorded measurements over the course of a set time period. To perform a kinetic measurement, open Galapagos as mentioned in section 3.2. 1. For kinetics measurements, click on the timer icon for timed measurements.
  • Page 13 (e.g. this is 5 seconds at a single wavelength for the EZ Read 800 Plus). Note: Method details of the kinetic experiment are listed on the left-hand panel of the screen.
  • Page 14 6. Users can also select to shake the microplate. Users can select shaking to be a single event or in timed cycles. 7. Select the amplitude (degree of linear shaking). 8. Select the speed for linear shaking. 9. Users can select the duration of shaking.
  • Page 15 The lines shown in each well refer to the measurement at one wavelength over time. Different wavelengths used for absorbance measurements are assigned a specific colour and identified by a legend.
  • Page 16 Results saved ‘Database’. A database is useful saving, sharing extracting data communication with external database system such as LIMS. Users can save files templates onto database, ‘File’ (save to own personal file), or ‘Save Method Template’ (saves the method protocol for future use). 11.

  • Page 17: Ulti Wavelength Measurement

    4 wavelengths for measurement. Each wavelength must be different. For the EZ Read 800 Plus ELISA, users can input wavelengths of 405, 450, 492 and 620nm. For the EZ Read Research model, users can choose four wavelengths from 405, 450, 492, 562, 595 and 620nm.
  • Page 18 3. Individual multi-wavelength measurements are displayed in each well, with raw absorbance displayed on the right hand panel as a graph of absorbance vs. wavelength. 4. Alternatively users can click on an individual well to view the scan in more detail and raw results.
  • Page 19 5. Results saved ‘Database’. A database is useful saving, sharing extracting data communication with external database system such as LIMS. Users can save files templates onto database, ‘File’ (save to own personal file), or ‘Save Method Template’ (saves the method protocol for future use).

  • Page 20: Calibrating The Instrument

    3.6 Calibrating the instrument The EZ Read 800 Plus performs an automatic calibration of the lamp energy before it measures a microplate; however, the user may use a calibration plate containing a series of neutral density filters with known optical density to verify reader performance. See Section 8: Ordering for order details.
  • Page 21 6. Incorrect entries can be removed using the  key. 7. The keypad can be locked to prevent unauthorized access. (default PIN is 1505). Service engineers can access service functions from here by entry of a specific PIN. 3.7.1 Description of the main menu options This option starts a measurement with the last used or defined 1.
  • Page 22 3.7.2.1 Method Definition: Filters Use the  keys to select the filter to be used for 1. Measurement measurement. Use the  keys to select the filter to be used as a reference. Select “none” for no filter. The reference value (usually 2.
  • Page 23 key. Press F4 to accept the entries. Press F1 ‘--->’ to select the well type required from the options listed at the bottom of the display box. With the exception of BK and ST the terms are names and whatever you want them to be.
  • Page 24 Used to see if results are outside pre-defined limits Low positive Used to see if results are outside pre-defined limits High positive Use as required. Control Use as required. Quality control Use for quantitative determinations; a maximum of 16 Standards standards can be used.
  • Page 25 3.7.2.3 Method Definition: Samples Samples can be placed either individually by moving the cursor to the desired location. Alternatively all wells not already used can be filled with samples. Replicate samples can be used in combination with single samples when desired. There is no limit on the replicate number. Press F3 to define default directions (horizontal or vertical) for replicates of samples and for different samples.
  • Page 26 1. The concentrations for the standards should be entered first. The screen offers as many entry fields as standards have been defined. 2. In case the standards are in linear serial dilution, the Quick key (F1) offers a quick way of entering the concentrations. 3.
  • Page 27 6. Press enter to move to the next method definition box. 3.7.2.5 Method Definition: Kinetics If kinetic measurements are to be defined, the Kinetics calculation definition window will appear after the layout has been set up. The calculated results for these kinetics options are normally used as the basis for quantitative, qualitative or combined sample evaluation.
  • Page 28 This option calculates the time at which the maximum slope is reached. A linear regression is performed for every three consecutive readings 3. Time of maximum slope over the whole measurement time. The center point in time of the regression with the steepest slope is the time the maximum slope.
  • Page 29 3. Press enter to move to the next method definition screen. Depending on the selected options different screens will be shown. NOTE: In case controls are used in replicates they must be referred as avg(xx), otherwise they are not recognized in a formula (xx should be replaced by the name of the control). 3.7.2.7 Method Definition: Factors 1.
  • Page 30 3. The next entry field is used to enter the condition for the elimination. For example individual positive controls should be eliminated which are deviating more than 15% from the mean value. The condition is entered as following: X > avg(PC) *1.15 | X < avg(PC) *0.85 X represents any single well of the positive control well, the ‘|’...
  • Page 31 1. There are three groups of elements that can be selected by pressing the F1 (Alt) key:  Controls (single and average) and the factors.  Operates +, -, /, etc.  All available mathematical and logical functions. 2. Use F2 and F3 to select the desired element and F4 to place it. NOTE: If a Blank (BK) is defined it will be automatically subtracted from the absorbencies of all other wells.
  • Page 32 3.7.2.10 Method Definition: Thresholds (Cut-off) Use this option to define the threshold limits for a qualitative result. Up to three different classes (ranges) are possible. The label for the classes can be user defined. For n thresholds you have n+ 1 quantitative label.
  • Page 33 3. The example below shows the setup for a typical ELISA assay. Here a sample is considered positive absorbance value is above (mean Positive control - Negative control) / 2. In addition it is specified that samples that are +/- 10% of the cut-off are considered as equivocal.
  • Page 34 3. For example for an ELISA assay the following criteria must be fulfilled:  The absorbance of the Standard 1 must be < 0.2.  The absorbance of the Standard 2 must be higher than that of the Negative Control. ...
  • Page 35 1. The new method can be appended to the list of already existing methods by scrolling down the list and pressing the Append (F4) key. 2. It is possible to store the new method at any position in the list by scrolling to the desired position for the new method.
  • Page 36 Serial Use if PC connected to send results through the serial port. None Use if neither printer nor PC are connected. Results are stored in the memory and can be recalled later using the Recall Plate Data option. This can speed up the measurement of a batch of plates significantly. 5.
  • Page 37 2. After a plate has been chosen press either F4 or the Enter key to load the data from the memory. 3. Further options include: Other Use a different method. Available methods are displayed (see earlier). Show Show the plate data Print OD Print the OD values obtained for the plate.
  • Page 38 6. The new screen shows the plate ID, original method absorbance (OD. value) selected position. On the right side of the screen there is a representation of a microplate. You can move across the plate with the cursor keys to select a particular well. At top of the plate representation the actual well location and the well designation is indicated.

  • Page 39: Technical Information

    For a technical description of the calibration process please refer to chapter 3. 3.7.5.3 Setup: Date/Time Use this menu option to change the date and time with  and the keypad. A choice of AM, PM or 24 hour clock is available. 3.7.5.4 Setup: Contrast Use this option to change the contrast of the liquid crystal display using the F1 and F2 keys.

  • Page 40: Scope Of Supply

    6.1 Approved Parts Except for the parts shown in the following list, only parts supplied by Biochrom or an authorized Biochrom Distributor may be installed in or used with the EZ Read 800 Plus. 6.2 Cleaning and Disinfection All parts of the reader that come into contact with potentially infectious material must be treated as potentially infectious and should be periodically clean and disinfected.

  • Page 41: Instrument Cleaning

     Lint-free tissues.  70% ethanol or a 0.5% bleach solution 6.3 Instrument cleaning The following cleaning procedure should be regularly performed and after any spillages. 1. Disconnect the instrument from the power supply and the PC. 2. Carefully wipe off the entire reader with lint-free tissues that have been moistened in a 70% ethanol or a 0.5% bleach solution.

  • Page 42: Returns Terms And Conditions

    Read 800 Plus Microplate Reader. The warranty shall lose effect if any of the below conditions occur:  Biochrom EZ Read 800 Plus Microplate Reader is not used in the defined scope of application.  Biochrom EZ Read 800 Plus Microplate Reader has obviously been damaged by external influences which are not in accordance with the provisions for the nominal range of use.

  • Page 43: Returns Procedure

    If your instrument is in warranty contact technical support: support@biochrom.co.uk If your instrument is out the warranty period, you must provide a purchase order number for the repair on your returns form. If you need to know that cost of repair/recalibration, please send an e-mail to support@biochrom.co.uk.
  • Page 44  Customs declaration of true value  Customs declaration: Temporary Import etc.  Have read and understood the returns terms and conditions (http://www.biochrom.co.uk/returns_terms.html)  For all returns enquires please contact returns@biochrom.co.uk or +44(0)1223 427861...

  • Page 45: Ordering Information And Accessories

    8. Ordering Information and Accessories Table 1 Ordering Information ORDERING INFORMATION 80-4002-02 Biochrom EZ Read 800 Plus ELISA 80-4002-03 Biochrom EZ Read 800 Plus Research SS01751 EZ Read Microplate Reader Check Plate See www.biochrom.co.uk for a list of filters that are available for purchase.

  • Page 46: Appendix

    Use high quality optically clear 96-well microplates (such as virgin polystyrene) with standard ANSI SBS footprint for the best results. 10.2 Troubleshooting and Frequently Asked Questions Biochrom’s technical support team is available to answer all of your questions regarding your EZ Read 800 Plus microplate reader: E-mail: support@biochrom.co.uk Telephone:...
  • Page 47 What is the indication range of the EZ Read 800 Plus and how does this specification differ from the linear range? The Biochrom EZ Read 800 Plus has an indication range of 0.000-3.300 OD and a linear range of 0.100-2.500 OD. We recommend that the broader indication range should be used only for relative determinations but not for determining the quantity of the absorbing material.
  • Page 48 does not absorb. 620nm is a frequently used reference wavelength in many absorbance applications in the visible range. Check the product data sheet for recommended reference wavelength for your specific assay. How can I connect the instrument to Galapagos? Refer to section 3.2.

  • Page 49: Declaration Of Conformity

    Declaration of Conformity for Biochrom Manufactured Products This is to certify that the following Biochrom manufactured products:- EZ Read 800 ELISA Plus (80-4002-02) and EZ Read 800 Research Plus (80-4002-03) Conform to the requirements of the following Directives:- 2006/95/EC Low voltage directive (LVD)

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