Summary of Contents for Aurora Scientific 206A
- Page 1 Olfactometer 206A Publish Date: 3/2/2017 Manual Version 1.1 www.AuroraScientific.com © Aurora Scientific, 2017 VER. 1.00 page 1...
- Page 2 CAUTION Maximum operating pressure is 30 psig (207 kPa). Do not exceed the maximum pressure ratings. Injury could result from use at higher pressures. Damage to the 206A may occur at pressures above the maximum rated pressure. © Aurora Scientific, 2017 VER.
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Page 3: Revision History
Olfactometer Instruction Manual Model: 206A Revision History Date Version Document Changes Number 20-Feb-2017 Initial Draft 3-Mar-2017 Added Chapter 8 Appendix Serial Communication Commands © Aurora Scientific, 2017 VER. 1.00 page 3... -
Page 4: Table Of Contents
Process Overview ..............................6 Quick Start Guide ..........................7 ..Instrument Setup ..............................7 2.1.1 Installation of the 206A Olfactometer ....................... 9 2.1.2 Using the 206A Olfactometer ........................... 10 2.1.3 Cleaning and Shutdown ............................ 11 General Operating Procedure ......................13 .. - Page 5 ..Technical Support Contact Information and Return Shipping Addresses ............39 ..Warranty ................................40 ..Returning Products to Aurora Scientific for Repair .................... 41 Specifications ..........................42 Appendix – Serial Communication Commands ................44 ..Commands ................................44 8.1.1 Valve .................................. 44 8.1.2 Vial ..................................
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Page 6: Introduction
1 Introduction 1.1 Scope and Purpose The 206A Olfactometer is an instrument designed to deliver a series of odor concentrations in a precisely controlled fashion. Careful fluid design of the instrument ensures that odors released have a well-defined “square” profile with minimal delay between activation of the valve and the arrival of the odor at the subject location. -
Page 7: Quick Start Guide
Olfactometer Instruction Manual Model: 206A 2 Quick Start Guide This quick start guide will provide you with basic information that will allow you to get your new 206A Olfactometer up and running as quickly as possible. 2.1 Instrument Setup The 206A Olfactometer is shipped in 1 or more boxes depending on how many extra vial modules you have purchased. - Page 8 Olfactometer Instruction Manual Model: 206A The following is a list of several items required in order to use the 206A Olfactometer. These items are not included with the 206A. Required Items Description Clean/Dry Air Source A source of clean and dry air is required by the 206A. This air can be supplied by various means including: high pressure gas bottle, lab air supply or a compressor.
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Page 9: Installation Of The 206A Olfactometer
USB port on the PC. 4. Pipe the air supply to the 206A Olfactometer. (For the purposes of this quick start guide we will assume that you are using a high pressure air cylinder as your air source and therefore there is no requirement for drying, filtering or removing odors from the air source.) -
Page 10: Using The 206A Olfactometer
Vial table above. f) Follow the same procedure to enter all odorants required by the study. Note: the 206A will auto detect the number of vials connected to the olfactometer and then grey out all vial numbers not present. -
Page 11: Cleaning And Shutdown
2. To perform a more complete cleaning do the following: a) Place the “Animal” outlet and “Exhaust” outlet of the Final Valve in a beaker for collection of liquid. © Aurora Scientific, 2017 VER. 1.00 page 11... - Page 12 Turn off the air source, close the valve on the high pressure air cylinder. c) Shut off the software by clicking on the X to close it or click on File>Exit. d) Power off the 206A by unplugging the DC power jack or by unplugging the AC adapter. © Aurora Scientific, 2017 VER.
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Page 13: General Operating Procedure
Mixing valve, and Final valve. Additional odor vial sections, each consisting of four vials and valves, can be added to the base 206A to increase the total vial count from 4 to 8 to 12. - Page 14 206A control program. The odor concentration released to the animal can be calculated as follows.
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Page 15: Odor Pulse Characteristics
A miniPID fast response photo-ionization sensor was used to measure the output odor pulse shape and timing produced by the 206A olfactometer. Figure 2 shows a typical trace, in this figure the green line shows the odor concentration and the red line shows when the Final valve was turned on and the off. -
Page 16: Instrument Setup
3.3 Instrument Setup 3.3.1 Unpacking the Instrument The 206A Olfactometer is shipped in 1 or more boxes depending on how many extra Odor Vial Subassemblies you have purchased. Unpack all boxes included with the shipment and check the contents against the list provided below. If there are any issues with what you received, please... - Page 17 Accessories for 206B including: 4 vials with septa. present with 206B) Table 3-1: Items included with a 206A Olfactometer The following is a list of several items required in order to use the 206A Olfactometer. These items are not included with the 206A. Required Items...
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Page 18: Initial Equipment Setup
USB port on the PC. 4. Pipe the air supply to the 206A Olfactometer. (For the purposes of this document we will assume that you are using a high pressure air cylinder as the air source and therefore there is no requirement for drying, filtering or removing odors from the air source.) -
Page 19: Loading A Vial
3.4.1 Insertion of Teflon hoses through Vial Septum An accessory kit is supplied with the 206A which includes a 16GA x 1.5” hypodermic needle that can be used to assist in inserting the 1/16” OD Teflon flow entry and exit tubes through the septum of the vial. -
Page 20: Filling And Installing A Vial
If not then remove the vial, and reduce the amount of liquid in the vial, and reinstall the vial on the cap before placing it back in the holder. 10) You are now ready to use the new vial. © Aurora Scientific, 2017 VER. 1.00 page 20... -
Page 21: Control Software
4 Control Software 4.1 Software Setup The 206A Olfactometer is supplied with a Software Install CD for Windows. Inserting this CD into a PC should launch the installer. Follow the on-screen wizard to complete installation of the control software. Following successful completion of the install wizard, the user should find an icon on the desktop to launch the control software. -
Page 22: Deliver An Odor
Olfactometer Instruction Manual Model: 206A f) Follow the same procedure to enter all odorants required by the study. Note: the 206A will auto detect the number of vials connected to the olfactometer and grey out all vial numbers not present. -
Page 23: Menus
4.3.2.2 Advanced Status and Control Menu Item Selecting this menu option will open the Advanced Status and Control Window. See section 4.6 for more details on the operation of this window. © Aurora Scientific, 2017 VER. 1.00 page 23... -
Page 24: Help Menu
Model: 206A 4.3.2.3 Valve Check Menu Item Selecting this menu option will sequentially open and then close all vial valves attached on the 206A. This menu is only used to quickly check correct operation of the olfactometer. 4.3.2.4 Sequencer Menu Item Selecting this menu option will open the Sequencer window. -
Page 25: Status Indicators
4.4.2 Controls The center portion of the window is occupied by a series of controls used to change the system output. © Aurora Scientific, 2017 VER. 1.00 page 25... - Page 26 4.4.2.5 Purge Button This button sets the valves to flow clean air only to the exhaust via the Mixing valve and closes all vials from the flow. © Aurora Scientific, 2017 VER. 1.00 page 26...
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Page 27: System Configuration Window
At the top of the window is a list box which describes the current setup of each vial. Note: the 206A will auto detect the number of vials connected to the olfactometer and grey out all vial numbers not present. - Page 28 Due to small discrepancies in the MFCs it is possible that the fresh air flow will not be © Aurora Scientific, 2017 VER. 1.00...
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Page 29: Advanced Status And Control Window
The software auto detects the number of vials connected to it and then updates the diagram to show all vials in the system. Figure 8 shows the System Status of a 206A with 4 vials while Figure 9 shows the same window with a 206A and a 206B installed for a total of 8 vials. -
Page 30: Sequencer Window
Model: 206A Figure 8: Advanced Status and Manual Control Window with 206A – 4 Vials Figure 9: Advanced Status and Manual Control Window with 206A and 206B – 8 Vials 4.7 Sequencer Window The Sequencer Window allows the user to create a sequence of challenges to be executed by the olfactometer with pre-defined timing. -
Page 31: Sequencer Controls
4.7.1.4 Move Down Button The Move Down button moves the selected row down by one row in the sequence. 4.7.1.5 Remove Button The Remove button removes the selected row from the sequence. © Aurora Scientific, 2017 VER. 1.00 page 31... - Page 32 Also, while a sequence is running, the Run button changes into an Abort button, which stops the running sequence immediately. At the conclusion of a running sequence, the main window is re-enabled and the Abort button changes back to Run. © Aurora Scientific, 2017 VER. 1.00 page 32...
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Page 33: Random Sequence Generator
The Included Vials checkboxes allow the user to select which of the attached vials should be included in the randomized challenge. The user can select all vials, some vials, or no vial as a valid option. Vials not © Aurora Scientific, 2017 VER. 1.00... - Page 34 Minimum Delay to Next Challenge (s) Data Entry Box The Minimum Delay to Next Challenge (s) data entry box allows the user to select the shortest delay the randomizer will wait between challenges in seconds. © Aurora Scientific, 2017 VER. 1.00 page 34...
- Page 35 The Generate button populates the Sequencer window with a randomly generated sequence that meets the parameters specified by the user and then closes the Random Sequence Generator window, see Figure 13. Figure 13: Example Challenge Sequence Generated by Random Sequence Generator © Aurora Scientific, 2017 VER. 1.00 page 35...
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Page 36: Calibration And Maintenance
Odor Delivery Duration on the main window to a long enough value that you can read the flow rate when the odor is switched, we suggest using 2000 – 5000 ms. Click on the Flow Odor to Exhaust button to start © Aurora Scientific, 2017 VER. 1.00... -
Page 37: Maintenance
950 sccm. Click on Flow Odor to Exhaust and let run for 30 minutes. 2) Repeat step (1) for each vial in the system. This should remove odor residue from the vial valves and vial tubing. © Aurora Scientific, 2017 VER. 1.00 page 37... - Page 38 Final valve as well. If you do replace these components then you must also replace all tubing and fittings after the mass flow controller subassembly. Contact Aurora Scientific to purchase replacement parts. © Aurora Scientific, 2017 VER. 1.00 page 38...
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Page 39: Performance Guarantee, Technical Support, Warranty And Repair Information
Our performance guarantee states: if for any reason a new product does not meet your research needs then you can return it to Aurora Scientific for exchange or a full refund. The performance guarantee only applies to new products and must be exercised within 60 days of receipt of the instrument. -
Page 40: Warranty
Products manufactured by Aurora Scientific Inc. are guaranteed to the original purchaser for a period of three (3) years. Under this warranty, the liability of Aurora Scientific is limited to servicing, adjusting and replacing any defective parts that are of Aurora Scientific manufacture. Aurora Scientific is not liable to the customer for consequential or other damages, labour losses or expenses in connection with or by reason of the use or inability to use the products manufactured by Aurora Scientific. -
Page 41: Returning Products To Aurora Scientific For Repair
SHALL BE VALID UNLESS MADE IN WRITING AND SIGNED BY AN EXECUTIVE OFFICER OF AURORA SCIENTIFIC INC. 6.5 Returning Products to Aurora Scientific for Repair There are a few simple steps that must be completed before returning your product to Aurora Scientific. Obtain a Return Material Authorization number (RMA#). Go to our website or contact our technical support department to obtain a RMA #. -
Page 42: Specifications
Weight [kg]: Dimensions [cm]: Mass Flow Control Module: 17L x 16W x 21H 4 CH Odor Module (206B): 17L x 16W x 21H Complete 206A: 38L x 16W x 21H 206A Software Recommended PC Specifications Processor: Quad-core Intel or AMD... - Page 43 Duration, Odor Delivery Duration Step Size, Minimum Delay to Next Challenge, Maximum Delay to Next Challenge, Delay to Next Challenge Step Size, Generate Stop Flow: Stops flow Help: About: Version number, contact information © Aurora Scientific, 2017 VER. 1.00 page 43...
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Page 44: Appendix - Serial Communication Commands
8 Appendix – Serial Communication Commands To communicate with a 206A, you must connect it to your PC via a USB port. The 206A will connect to the PC and appear as a serial COM port. The PC operating system will normally assign the connection to either COM3 or COM4. -
Page 45: Vial
Olfactometer Instruction Manual Model: 206A 8.1.2 Vial The vial command is used to set the state of a pair of valves on the 206A connected to each vial. The vials are numbered as follows: Vial Number Location/Function 1–3 MFC board... -
Page 46: Findmodules
Olfactometer Instruction Manual Model: 206A 8.1.4 findModules The findModules command is used to determine how many vial modules are connected to the 206A. Command findModules <I2C ADDR> e.g. findModules 1 Description: Inquire number of modules attached to olfactometer at address 1. - Page 47 Olfactometer Instruction Manual Model: 206A 2 = External Thermistor Command temp <I2C ADDR> <SENSOR ID> e.g. temp 1 1 Description: Inquire temperature of onboard thermistor. Successful Reply 26.43 © Aurora Scientific, 2017 VER. 1.00 page 47...
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