Covaris AFA ultraCUBE User Manual

Water management system

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AFA ultraCUBE Water Management

A system developed for maintaining bath water quality in Covaris

AFA™ instrumentation

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AFA ultraCUBE User Manual

System

USER MANUAL

AFA ultraCUBE

500577

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Page 1 AFA ultraCUBE Water Management System USER MANUAL A system developed for maintaining bath water quality in Covaris AFA™ instrumentation Product Name: AFA ultraCUBE Product Number: 500577 AFA ultraCUBE User Manual...
Page 2 Registered names and trademarks used in this document, even when not specifically marked as such, are not to be considered unprotected by law. WCS 2.0 and AFA are either registered trademarks or trademarks of Covaris, Inc. in the United States and/or other countries.
Page 3 Covaris personnel. If Covaris personnel are required to perform on-site repair, all travel costs are paid by the customer. A limited warranty as specified may apply to certain components of the equipment.
Page 4 This page intentionally left blank. AFA ultraCUBE User Manual...
Page 5: Table Of Contents
Installation and Setup ....................10 Indicators and Controls ....................... 12 System Operation ........................14 Specifications ..........................15 Service ............................16 Appendix B – User Guidelines and Troubleshooting ................23 Appendix C – Bio-safety References ...................... 23 AFA ultraCUBE User Manual...
Page 6: Introduction
INTRODUCTION Overview of the Manual This manual contains operation and service instructions for the Covaris ultraCUBE Water Management System. It contains background information essential to the proper use and care of this equipment. Should any unforeseen problems occur with the normal operation of the equipment, contact Covaris Technical Support immediately.
Page 7 Do not remove safety labeling. To prevent damage to the equipment: 1. The AFA ultraCUBE system is designed for indoor use and to operate in typical ambient laboratory conditions e.g., 19°C to 25°C (66°F to 77°C) DO NOT operate the instrument in a cold room environment.
Page 8 Product Description Overview The AFA ultraCUBE is Covaris’ most advanced water management solution for the Covaris line of Focused-ultrasonicators. It combines a high performance solid state CH05 chiller with the Covaris WCS 2.0 water conditioning system, consisting of a UV lamp for controlling biological growth with a 5 micron particulate filter.
Page 9 The particulate filter is rated for 5 micron, so will catch most particulates and many cell types. The Covaris CH05 chiller and WCS 2.0 operate in combination to circulate the instrument’s bath water through the WCS 2.0 ultraviolent (UV) sterilizer and particulate filter to ensure that the water remains clean and free of algae growth for up to one month.
Page 10: Ultracube Installation And Setup
Water Tank WCS 2.0 ports. Make certain that sufficient air flow is available for cooling the CH05. Note: Previous Covaris products used low pressure black neoprene hose. Because the pump in the CH05 chiller can generate more pressure than the black hose can tolerate, the ultraCUBE comes with a white, high pressure hose.
Page 11 AFA ultraCUBE User Manual...
Page 12: Indicators And Controls
Indicators and Controls There are four operating status indicators on the front panel of the WCS 2.0, as shown. Maintenance Lamp Hour Meter Power Switch Detail of the WCS 2.0 Status Indicators AFA ultraCUBE User Manual...
Page 13 Cooling is indicated by a “-“ at the left of the graphic display; heating is indicated by a “+”; and standby is indicated by an “*”. When in Standby mode, the CH05 continues to pump bath water through the WCS 2.0, but the heating/cooling element is inactive. AFA ultraCUBE User Manual...
Page 14: System Operation
“*” is displayed at the far left of the CH05 display to place the CH05 in standby. When employing the WCS 2.0 with a Covaris water bath, users may refrain from changing bath water for up to one month. Please note that evaporation will occur, so the bath water level should be verified daily and distilled or de-ionized water added to the bath as needed.
Page 15: Specifications
EMC Low Voltage Directive 2006/95/EC Directive 2004/108/EC, EN Emissions & Immunity EN 61326-1: 61236-1, FCC Part 15, and 2006 ICES-003 for Class A Harmonics Emissions EN 61000-3-2: equipment. 2006 Voltage Fluctuations & Flicker EN 61000-3-3: 2008 AFA ultraCUBE User Manual...
Page 16: Service
The UV lamp and particulate filter are designed for replacement by the user following a MAINTENANCE indication on the WCS 2.0 front panel. There are no other user-serviceable repairs. Please contact Covaris or your local Covaris representative for assistance. Please direct any queries to: ...
Page 17 UV lamp and filter be replaced as soon as is practical after the MAINTENANCE indicator illuminates. Once these items have been replaced, the maintenance alert should be reset using an internal RESET button. Replacement accessories are available from Covaris using the following part numbers:  500175 - Replacement Water Filter ...
Page 18 7. With the two O-rings positioned about a half-inch from the top of the quartz tube, slide the tube into the UV chamber. 8. Thread the white cap onto the chamber by turning clockwise until snug. Hand tighten only. 9. Reinstall UV lamp. AFA ultraCUBE User Manual...
Page 19 THE ULTRAVIOLET LAMP CONTAINS MERCURY (Hg). Handle carefully to avoid breaking the lamp. Dispose of used lamps according to disposal laws. For further information, see one of the following websites or contact Covaris. www.lamprecycle.org (North America) www.lamprecycling.com...
Page 20 UV Plug and Lamp Particulate Filter Replacement WARNING: If the Covaris system has been employed for processing of infectious or hazardous biological samples, laboratory gloves should be worn and the used filter cartridge treated as biohazard waste, in case any biological sample accidentally entered the bath and was captured by the filter system.
Page 21 WCS 2.0 cabinet. Reconnect the fittings as originally found by pressing them into each Quick Connect socket until they snap securely. AFA ultraCUBE User Manual...
Page 22 5. With the WCS 2.0 cover off, verify that the UV lamp enclosure is fully closed (the black grommet covers the opening and the UV lamp is not visible). Fill the Covaris bath with distilled or de-ionized water, and reconnect the 12V power module. Turn ON the WCS 2.0 and CH05.
Page 23: Appendix B - User Guidelines And Troubleshooting
Clinical and Laboratory Standards Institute (CLSI), M29-A4 Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline, Fourth Edition, published 2014, ISBN 1-56238-961-  Occupational Safety and Health Administration (OSHA), 29 CFR 1910.1030 Bloodborne Pathogens  International Standards Organization (ISO) 15190:2003, Medical Laboratories – Requirements for Safety AFA ultraCUBE User Manual...