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Spectroquant Pharo 300 Manual

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Table of Contents

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Summary of Contents for Spectroquant Pharo 300

  • Page 1 Spectroquant ® Pharo 300 oquant ® Pharo 300...
  • Page 2 Operating manual Spectroquant UV/VIS Spectrophotometer ® Pharo 300...
  • Page 3 Spectroquant UV/VIS Spectrophotometer ® Pharo 300 General Information...

  • Page 4: Table Of Contents

    Spectroquant ® photometers Contents 1 Photometers ........

  • Page 5: Photometers

    Spectroquant ® photometers 1 Photometers 1.1 Photometry When a beam of light is transmitted through a colored solution, then this beam loses its intensity, in other words a part of the light is absorbed by the solution. Depending on the substance in question, this absorption occurs at specific wave lengths.

  • Page 6: The Photometers

    Spectroquant ® photometers 1 Photometers 1.2 The Photometers The photometers that belong to the Spectroquant ® Analysis System differ from con ven tional photo meters in the following important aspects: • The calibration functions of all test kits are electronically stored.

  • Page 7: Spectroquant ® Cell Tests

    Spectroquant ® photometers 2 Photometric Test Kits ® 2.1.1 Spectroquant Cell Tests Identification mark for the correct insertion into the cell Leakproof cap compartment of the photo- meter Bar code for identification in the photometer Cat. No. of test kit...

  • Page 8: Notes For Practical Use

    In stead of the sample volume, the corresponding quantity of distilled or DI water is used. This reagent blank value is prestored in the ® photometers belonging to the Spectroquant analysis system, which means that - due to the high batch reproducibility - it is possible to dis- pense with a separate measurement of the reagent blank.

  • Page 9: Influence Of Ph

    Spectroquant ® photometers 2 Photometric Test Kits 2.2.2 Influence of pH Chemical reactions follow an optimal course only within a certain pH range. The rea gents contained in the test kits produce an adequate buf- fering of the sample sol u tions and ensure that the pH optimal for the reac- tion in question is obtained.

  • Page 10: Influence Of Foreign Substances

    Spectroquant ® photometers 2 Photometric Test Kits 2.2.5 Influence of Foreign Substances Foreign substances in the sample solution can • raise the measurement value as a result of an amplification of the reaction • lower the measurement value as a result of a prevention of the reaction.

  • Page 11: Shelf-Life Of The Reagents

    2 Photometric Test Kits 2.2.7 Shelf-life of the Reagents The Spectroquant ® test kits are in most cases stable for 3 years when stored in a cool, dry place. A few test kits have a lower shelf-life of 18 or 24 months or must else be stored in a refrigerator.

  • Page 12: Sample Preparation

    Spectroquant ® photometers 3 Sample Preparation 3.2 Preliminary Tests Correct measurement results can be obtained only within the measuring range spe ci fied for each indi vi dual parameter. When dealing with sample solutions of an un known concentration, it is advisable to establish whether the sample concentration is indeed within the specified measuring range, ideally roughly in the middle of the range.

  • Page 13: Dilution

    Spectroquant ® photometers 3 Sample Preparation All dilutions should be made in such a way that the measurement value lies in the middle of the measur ing range. As a rule, the dilution factor should never be higher than 100. In the event that yet higher dilu tions become necessary all the same, then this must be done in two separate steps.

  • Page 14: Homogenization

    Spectroquant ® photometers 3 Sample Preparation Procedure for microfiltration Draw out the liquid Screw the syringe Hold the syringe Filter the contents to be filtered with tightly into the front upright and slowly of the syringe into the syringe. side of the mem -...

  • Page 15: Decomposition

    (capacity: 12 or 24 decomposition cells) at 120 °C or, respec- tively, 100 °C. Details regarding the heating times and further treatment ® can be found in the package inserts contained in the Spectroquant Crack Set packs. Determination of...

  • Page 16: Pipetting System

    (weighing ac cu racy ±1 mg), 1 ml of water at 20 °C = 1.000 g ±1 mg ® b) using Spectroquant PipeCheck; this is a pho tometric check of the pipette, and scales are not necessary (see section “AQA”).

  • Page 17: Quality Control At The Manufacturer

    Spectroquant ® photometers 5 Analytical Quality Assurance (AQA) The objective of analysis must always be to determine the true content of the analyte in question as accurately and precisely as possible. Analytical Quality Assurance represents a suitable and indispensible method by which the quality of the user's own work can be assessed, errors in the measurement system diagnosed, and the comparability with the results obtained using the respec tive refe rence methods demonstrated.

  • Page 18: Analytical Quality Assurance (Aqa)

    Lot Certificate / Certificado del lote ® Spectroquant CSB-Küvettentest ® ® Spectroquant COD Cell Test / Spectroquant Test en cubetas DQO Calibration function: Art.Nr. / Cat.No. / Art. Nro. 1.14560.0001 n = 10 Sollwert Messergebnis...

  • Page 19: Quality Control For The User

    Test for the overall system includes checking the working equipment and checking the handling operations. The overall system can be checked using standard solutions of a known ® content, preferably with the Spectroquant CombiCheck; this corres ponds with the AQA 2 mode in the photometer. ®...

  • Page 20: Checking The Overall System

    5.2.3 Checking the Pipettes ® The Spectroquant PipeCheck is used to check the pipettes. The pack contains cells filled with color-dye concentrates. After the addition of a predefined volume of water using the pipette in question, the cell is meas- ured against a corre sponding reference cell also contained in the pack.

  • Page 21: Testing For Handling Errors

    It is recommended to check for any influence by using ® the addition solution contain ed in the Spectroquant CombiCheck pack. A defined quantity of the addition solution (R-2), which contains a known concen tration of the respective analyte, is added to the sample and the recovery rate is de termined.

  • Page 22: Definition Of Errors

    Spectroquant ® photometers 5 Analytical Quality Assurance (AQA) 5.4 Definition of Errors It is obvious that measurement results as a rule may be associated with errors. This applies equally to standardized methods of analysis (reference methods) and to rou tine analysis. The discovery and the minimization of errors must be the objective here.
  • Page 23 Spectroquant UV/VIS Spectrophotometer ® Pharo 300 Description of Function...
  • Page 24 ® Spectroquant Pharo 300 Accuracy when going to The use of advanced technology and the high quality standard of our press instruments are the result of continuous development. This may result in differences between this operating manual and your instrument.
  • Page 25 Spectroquant Pharo 300 - Contents Overview ........29 Overview of the instrument .
  • Page 26 Contents Spectroquant Pharo 300 Zero adjustment ....... 61 Measuring in Concentration mode ....66 Measuring cell tests with barcode .

  • Page 27: Spectroquant

    ® Spectroquant Pharo 300 Contents 4.11.5 Saving measurement datasets automatically . 145 4.11.6 Displaying measurement data memory ..146 4.11.7 Filtering measurement datasets ... 148 4.11.8 Inverting filters .
  • Page 28 Contents Spectroquant Pharo 300 Maintenance and cleaning ..... 191 Exchanging the buffer batteries ....191 Cleaning .

  • Page 29: Overview

    ® Spectroquant Pharo 300 Overview Overview Overview of the instrument Front of the instrument 1 Display 2 Keypad 3 Shaft for rectangular cells 4 Turn-up lid 5 Shaft for round cells 6 Cell shaft cover Fig. 1-1 Front of the instrument with operating elements...

  • Page 30: Keypad

    ® Overview Spectroquant Pharo 300 Keypad Overview 1 Function keys F1 to F4 (function menu-depending) 2 Alphanumeric keypad 3 Keys with dedicated function Fig. 1-3 Keypad Key functions The keys on the right side of the keypad have the following functions: Designation Functions <ON/OFF>...
  • Page 31 ® Spectroquant Pharo 300 Overview Designation Functions <TIMER> – Opens the menu, Timer. <ESC> – Cancels the running action. Entries that have not yet been accepted are discarded. – Switches to the next higher menu level. <START ENTER> – Starts an action (e.g. measure- ment) –...

  • Page 32: Display

    ® Overview Spectroquant Pharo 300 Display Display elements Concentration 16.04.07 9:52 1.92 mg/l 18: 14752 10 mm 0.05 - 3.00 mg/l Setup Citation form Unit Method list 1 Status line (current state, date and time) 2 Display range for menus and measurement results 3 Function keys menu Fig.

  • Page 33: Safety Instructions

    ® Spectroquant Pharo 300 Safety instructions Safety instructions This operating manual contains basic instructions that you must follow during the commissioning, operation and maintenance of the photometer. Conse- quently, all responsible personnel must read this operating manual carefully before working with the meter. Keep this operating manual in the vicinity of the meter.

  • Page 34: Authorized Use

    ® Safety instructions Spectroquant Pharo 300 Authorized use The Photometer was developed for use in the laboratory for water analysis. Follow the technical specifications of the cells in chapter 7 T ECHNICAL DATA Any other use is considered to be unauthorized.

  • Page 35: Handling Of Hazardous Substances

    ® Spectroquant Pharo 300 Safety instructions Handling of hazardous substances When developing test sets, Merck carefully sees that the tests can be carried out as safely as possible. Some hazards by dangerous substances, however, cannot always be avoided. WARNING Improper handling of certain reagents can cause damage to your health.
  • Page 36 ® Safety instructions Spectroquant Pharo 300 ba75703e07 04/2014...

  • Page 37: Commissioning

    ® Spectroquant Pharo 300 Commissioning Commissioning Scope of delivery ®  Spectrophotometer Spectroquant Pharo 300  Power pack connection cable  Buffer batteries 4 x AA alkaline manganese (Mignon)  Zero cell (16 mm, round)  Short instructions  CD-ROM with –...

  • Page 38: General Notes On Handling

    General notes on handling ® The Spectroquant Pharo 300 photometer is an optical precision meter. Therefore, it should always be handled with care, especially in mobile use. Always protect the meter from conditions that could damage the mechanical, optical and electronic components. Heed the following points especially: ...

  • Page 39: Initial Commissioning

    When you set the language, date and time according to the mentioned sec- tions of this operating manual you will quickly become familiar with the simple ® operation of the Spectroquant Pharo 300. More detailed instructions on operation are given in section 4.2 G ENERAL OPERATING PRINCIPLES 3.3.1...

  • Page 40: Connecting The Power Supply

    ® Operation with a You can also operate the Spectroquant Pharo 300 on the move and inde- mobile 12 V power pendent of the local power supply. source To do so, a 12 V power supply such as a commercial 12 V portable power source or a 12 V car battery and the 12 V-Adapter available as an accessory is required (see section 8.1).

  • Page 41: Switching On The Photometer For The First Time

    ® Spectroquant Pharo 300 Commissioning 3.3.3 Switching on the photometer for the first time During the initial commissioning, the photometer automatically guides you through the setting of the meter language, date and time after switching on (see following sections). 1 Press <ON/OFF>.

  • Page 42: Setting The Date And Time

    ® Commissioning Spectroquant Pharo 300 3.3.5 Setting the date and time During the initial commissioning, the instrument automatically guides you to the setting of the time and date after the setting of the language. The Date/Time menu is open. Date/Time 16.04.07 9:52...

  • Page 43: Connecting Optional Accessories

    ® Spectroquant Pharo 300 Commissioning Connecting optional accessories 3.4.1 Communication interfaces Connections RS232 USB-A USB-B figure 3-1 Communication interfaces on the rear panel You can connect the following accessories to the photometer:  PC (see section 3.4.2)  Printer (see section 3.4.2) ...

  • Page 44: Pc/Printer

    ® Commissioning Spectroquant Pharo 300 3.4.2 PC/printer PC and printer can be connected to the photometer as follows: Interface Printer Functions   RS232 The data is sent to the interface with <PRINT>.  If a printer is connected, the data is printed out.

  • Page 45: Usb Memory Device

    ® Spectroquant Pharo 300 Commissioning Operation at RS232 Connect the RS232 interface to the devices as follows:  PC: with a commercially available zero modem cable  Printer: with a commercially available RS232 printer cable The cables are available in specialized computer shops.

  • Page 46: Pc Keyboard

    ® Commissioning Spectroquant Pharo 300 3.4.4 PC keyboard With the PC keyboard it is possible to enter letters, e.g. to assign names for identification (ID). In addition, the following keys of the PC keyboard are assigned with the fol- lowing functions of the photometer:...

  • Page 47: Adapter

    Commissioning 3.4.6 12 V-Adapter ® With the 12 V-Adapter you can operate the Spectroquant Pharo 300 spec- trophotometer on the move and independent of the local power supply. To do so, a 12 V power supply such as a commercial 12 V portable power source or a 12 V car battery is required.
  • Page 48 ® Commissioning Spectroquant Pharo 300 ba75703e07 04/2014...

  • Page 49: Operation

    ® Spectroquant Pharo 300 Operation Operation Switching on or off the photometer Switching on 1 Switch the photometer on with <ON/OFF>. The display shows Self test 16.04.07 9:52 – the Self test dialog (if the user management is not active).
  • Page 50 ® Operation Spectroquant Pharo 300 The self-test includes: Self test 16.04.07 9:52 – the test of the memory, proces- Keep cover closed sor, internal interfaces, System test filter and lamp Filter test Lamp test – a calibration for each wave-...
  • Page 51 ® Spectroquant Pharo 300 Operation AutoCheck With the AutoCheck function the photometer checks and calibrates the opti- cal measuring unit. The AutoCheck is automatically carried out if measure- ment settings were changed since the last measurement, e.g.:  if a different wavelength was selected or ...

  • Page 52: General Operating Principles

    ® Operation Spectroquant Pharo 300 General operating principles 4.2.1 Navigating with function keys and menus Main menu Press < > twice Function key Moves the selection down menu by 2 positions Press the F1 Funktion key ("Settings") Current selection in reverse video Opens the "Settings"...
  • Page 53 ® Spectroquant Pharo 300 Operation Use of the function The function keys F1 to F4 are below the display. Their functions change keys depending on the operating situation and mode. The current functions are displayed in the function key menu at the bottom edge of the display.

  • Page 54: Display Of Navigation Paths In Short Form

    ® Operation Spectroquant Pharo 300 4.2.2 Display of navigation paths in short form In this operating manual, the introductory navigation steps leading to individ- ual menus or dialogs are clearly shown in a gray box. The box indicates a section of the menu tree.

  • Page 55: Entry Of Numerals, Letters And Characters

    ® Spectroquant Pharo 300 Operation Note The complete menu tree is given in the appendix of this operating manual. 4.2.3 Entry of numerals, letters and characters Numerals, letters, punctuation marks and special characters are entered with the alphanumeric keypad of the meter or using an external keyboard.
  • Page 56 ® Operation Spectroquant Pharo 300 Note During mere number entries (such as entering a wavelength), the keys of the alphanumeric keypad are assigned to the respective numeral only. Each key- pressing directly enters the numeral (like a pocket calculator). Special characters Special characters are entered with the <1/*>...

  • Page 57: Detailed Operating Example: Changing The Language

    ® Spectroquant Pharo 300 Operation 4.2.4 Detailed operating example: Changing the language 1 Call up the main menu with the <HOME> key. 2 Open the General setup menu with the F1 function key [Setup]. 3 Using <><> select the Lan- General setup 16.04.07 9:52...

  • Page 58: Photometer Settings And System Administration

    ® Operation Spectroquant Pharo 300 Photometer settings and system administration The general photometer settings are done in the <HOME> -> General setup menu. The general photometer settings comprise:  Language (see section 4.3.1)  Date/time (see section 4.3.2 and section 4.2.4) ...

  • Page 59: Date/Time

    ® Spectroquant Pharo 300 Operation 4.3.2 Date/Time The date format is set automatically with the language setting. According to the locally usual version, the date format is displayed in the order, Day.Month.Year (DD.MM.YY) or Month/Day/Year (MM/DD/YY or MM.DD.YY). <HOME> [General setup] –...

  • Page 60: Display Settings

    ® Operation Spectroquant Pharo 300 4.3.3 Display settings Here you can adjust the display contrast to the lighting conditions. <HOME> [General setup] – Display settings 1 Select and confirm Contrast. Display settings 16.04.07 9:52 Contrast 50 % A slide control for the display con- trast appears.

  • Page 61: Zero Adjustment

    Kinetics. With a zero adjustment, the absorbance of a cell filled with distilled water ("zero cell") is measured and stored. ® Factory zero For all measurements with Spectroquant test sets (Concentration mode), a adjustment for factory zero adjustment is available in the delivery condition. We recommend concentration replacing it with a zero adjustment of your own.
  • Page 62 8 A are especially adapted CCESSORIES AND OPTIONS ® to the Merck Spectroquant test set program. General requirements of the cells are given in chapter 7 T . Note that the spectral transpar- ECHNICAL DATA ency of the cell must be suitable for the intended application (example, quartz cell for UV range).
  • Page 63 ® Spectroquant Pharo 300 Operation Carrying out a zero The zero adjustment takes place similarly in the Concentration, Absorbance adjustment / % Transmission, Special / Multi wavelengths and Kinetics modes. 1 In the respective mode, press the <BLANK ZERO> key.
  • Page 64 ® Operation Spectroquant Pharo 300 Rectangular cell: Open the inner turn-up lid. Zero cell (H O dist.) Insert the rectangular cell verti- cally so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back.
  • Page 65 ® Spectroquant Pharo 300 Operation Validity of the zero The data of the zero adjustment is stored in the photometer separately for adjustment each cell type. As long as the data is valid, it is automatically used again after a temporary change to a different cell type. The validity depends on the...

  • Page 66: Measuring In Concentration Mode

    ® Operation Spectroquant Pharo 300 Measuring in Concentration mode 4.5.1 Measuring cell tests with barcode <HOME> Concentration Inserting a cell with barcode starts Concentration 16.04.07 9:52 a measurement. Please select method for measuring or insert a barcoded cell or insert AutoSelector.

  • Page 67: Measuring Reagent Tests With Autoselector

    ® Spectroquant Pharo 300 Operation 4.5.4). 4.5.2 Measuring reagent tests with AutoSelector <HOME> Concentration The method is selected by insert- Concentration 16.04.07 9:52 ing the AutoSelector. Please select method for measuring or insert a barcoded cell or insert AutoSelector. Setup...

  • Page 68: Measuring Reagent-Free Tests And User-Defined

    ® Operation Spectroquant Pharo 300 3 Open the inner turn-up lid. 4 Insert the rectangular cell verti- cally so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back.
  • Page 69 ® Spectroquant Pharo 300 Operation 1 Select the method manually Concentration 16.04.07 9:52 (see section 4.5.5). Please select method for measuring or insert a barcoded cell or insert AutoSelector. Setup Method list Last method New Method The photometer is ready to mea- Concentration 16.04.07 9:52...
  • Page 70 ® Operation Spectroquant Pharo 300 Rectangular cell: Open the inner turn-up lid. Insert the rectangular cell verti- cally so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back.

  • Page 71: Exceeding The Upper Or Lower Limits Of The Measuring Range

    ® Spectroquant Pharo 300 Operation 4.5.4 Exceeding the upper or lower limits of the measuring range Measured value display if the measured value is outside the measuring range: Range Display Example: MR: 10 - 150 mg/l LL < MV < UL Measured value UL <...

  • Page 72: Selecting A Method Manually

    ® Operation Spectroquant Pharo 300 4.5.5 Selecting a method manually Selecting the The method last used is immedi- method last used <HOME> ately selected. Concentration – [Last method] Selecting a method from the Method list <HOME> Concentration – [Method list] The list of methods is displayed.

  • Page 73: Settings For Concentration Mode

    ® Spectroquant Pharo 300 Operation Search function Search for a character string: Select method (last used) 16.04.07 9:52 Enter the character string to be searched for in the search window 14540 10 - 150 mg/l 14541 25 - 1500 mg/l with <A...9>.
  • Page 74 ® Operation Spectroquant Pharo 300 Overview of the Menu item Explanation settings Dilution Here you can set the dilution prior to measuring if you want to use a diluted sample. In the measured value display, the dilution is indi- cated in the form [1 + x] (parts sample + parts dis- tilled water).

  • Page 75: Measuring Diluted Samples

    ® Spectroquant Pharo 300 Operation 4.5.7 Measuring diluted samples If the concentration of a sample exceeds the measuring range of a method, you can specifically dilute the sample so that the concentration of the diluted sample is in the measuring range of the method. Thus a valid measurement is possible.
  • Page 76 ® Operation Spectroquant Pharo 300 1 Open the setting menu with Concentration 16.04.07 9:52 [Setup]. 2 Select and confirm Dilution. The input field for the dilution pops Sample + distilled water 1 + _ 3 Enter and confirm the dilution (<0...9>).

  • Page 77: Sample Blank Value

    ® Spectroquant Pharo 300 Operation 4.5.8 Sample blank value By measuring and using a sample blank value, measurement errors due to coloring and turbidity of the sample matrix can be eliminated to a large extent. The sample blank value is a characteristic of the sample (coloration) to be currently determined.
  • Page 78 ® Operation Spectroquant Pharo 300 3 Insert the cell with a suitable blank Sample blank value 16.04.07 9:52 sample. The first single measurement for the sample blank value takes To start measurement, insert cell or press <START/ENTER> place. The following data is displayed as...

  • Page 79: Reagent Blank Value

    With photometric concentration determination, the reagent blank value is a ® defined reagent constant. The method data for all measurements with Merck Spectroquant blank values test sets (Concentration mode) include an exactly determined reagent blank value. This value is overwritten if you measure the reagent blank value your- self (setting, User-defined blank value, see section 4.5.6).
  • Page 80 ® Operation Spectroquant Pharo 300 User-defined For user-defined methods, you can activate the reagent blank value function methods as follows only: Entry type Function type Reagent blank value possible? Entry of a function Linear (with and without entering the ordi-...
  • Page 81 ® Spectroquant Pharo 300 Operation Measuring the <HOME> reagent blank value Concentration Inserting a cell with barcode starts Concentration 16.04.07 9:52 a measurement. Please select method for measuring or insert If a cell without barcode is used: a barcoded cell or insert AutoSelector.
  • Page 82 ® Operation Spectroquant Pharo 300 3 Insert the cell with the blank sam- Blank value 16.04.07 9:52 ple. The first single measurement for the reagent blank value takes To start measurement, insert cell or press <START/ENTER> place. The following data is displayed as...

  • Page 83: User Calibration (Standard Adjustment)

    ® Spectroquant Pharo 300 Operation 4.5.10 User calibration (standard adjustment) Some methods for concentration measurement provide the option to opti- mize the original calibration stored with the method by means of a user cali- bration. When creating a used-defined method you can also allow a user calibration (see section 4.5.12).
  • Page 84 ® Operation Spectroquant Pharo 300 Carrying out a user <HOME> calibration Concentration Select the method manually Concentration 16.01.12 9:52 (see section 4.5.5). If there are already data for the zero adjustment, reagent blank Please select method for measuring or insert value or a user calibration avail- a barcoded cell or insert AutoSelector.
  • Page 85 ® Spectroquant Pharo 300 Operation 3 In the Target value column, enter Calibrate the method 16.01.12 9:52 the nominal values of the individ- Target value (Ca) Absorbance ual standard solutions. 0.00 mg/l 0.60 mg/l 1.50 mg/l The nominal value for E0 (reagent 2.40 mg/l...
  • Page 86 ® Operation Spectroquant Pharo 300 8 In the Absorbance column, select Calibrate the method 16.01.12 9:52 all fields one after the other and Target value (Ca) Absorbance start the respective measurement 0.00 mg/l E 0.177 0.60 mg/l 1 0.433 with <START ENTER>.
  • Page 87 ® Spectroquant Pharo 300 Operation 12 Enter the Lot number of the Calibrate the method 16.01.12 9:52 reagent blank value (<A...9>) and confirm. User calibration: The user calibration is completed. Protocol ID: Lot number for reagent blank E0 Date: 16.01.2012...
  • Page 88 ® Operation Spectroquant Pharo 300 Viewing the data of the user calibration <HOME> Concentration Select the method manually Concentration 16.01.12 9:52 (see section 4.5.5). If there are already data for the zero adjustment, reagent blank Please select method for measuring or insert value or a user calibration avail- a barcoded cell or insert AutoSelector.
  • Page 89 ® Spectroquant Pharo 300 Operation Measuring with user calibration <HOME> Concentration Select the method manually Concentration 16.01.12 9:52 (see section 4.5.5). [Cal][BV/2c][ZERO 10.01.2012 11:08] If there are already data for the User calibration A calibration dated xxx is available for this...

  • Page 90: Automatic Turbidity Correction

    ® Operation Spectroquant Pharo 300 4.5.11 Automatic Turbidity correction The Turbidity correction function activates the automatic recognition and compensation of the light absorption caused by turbid substances. After activating the function remains permanently switched on. Measured val- ues that were measured with Turbidity correction are labeled with [TURB] (turbidity correction) on the display and in the documentation (printout and memory).
  • Page 91 ® Spectroquant Pharo 300 Operation Nominal value (e. g. concentration) figure 4-2 Example of a linear calibration function after a 10-point calibration In the case of a linear dependency, the calibration function is determined by means of linear regression. The slope and axis intercept (E0) are the charac- teristics of the calibration line.
  • Page 92 ® Operation Spectroquant Pharo 300 Determining the You have the following options to create a method: calibration function  Measure and store: Carry out a series of measurements with the following sample solutions while at the same the photometer takes over the values: –...
  • Page 93 ® Spectroquant Pharo 300 Operation If the exact value for a0 is not known at the time the formula is entered, it is sufficient to enter the coefficient a1. In this case, the User-defined blank value function (in the Concentration / Setup menu) has to be activated to measure with this method.
  • Page 94 ® Operation Spectroquant Pharo 300 Further method data Input field Possible entries Number* 1001 ... 1100 Designation Any name (max. 18 characters) Version Any version designation (max. 18 characters) Wavelength* Freely selectable (in nm) Cell* 16 (round), 10, 20 or 50 mm Citation form e.g.
  • Page 95 ® Spectroquant Pharo 300 Operation How to program <HOME> user-defined Concentration methods – [Setup] – New method 1 Enter the general method data Edit method 16.04.07 9:52 here. The next available method number is already entered as the Number 1001...
  • Page 96 ® Operation Spectroquant Pharo 300 Variant 1: 1 Select and confirm Measure stan- Edit method 16.04.07 9:52 Measure standard dard solutions. solutions Standard ID 2 Enter and confirm details of the Standard manufacturer standard solutions (optional). 3 Using [Next], accept all entries on the page and switch to the next page.
  • Page 97 ® Spectroquant Pharo 300 Operation The measurement display Absorbance E0 16.04.07 9:52 appears. 7 Insert the cell with the respective standard. To start measurement, insert cell or press <START/ENTER> The absorbance is measured. The result of the first single measure- ment is displayed.
  • Page 98 ® Operation Spectroquant Pharo 300 The related formula f(x) and corre- Edit method 16.04.07 9:52 lation coefficient R are displayed f(x)=0.73x Meas. range: 0.050 - 1.000 mg/l = 1.000 above the diagram. 12 If required, select a different line type for the line adjustment with [Curve type].
  • Page 99 ® Spectroquant Pharo 300 Operation Variant 3: 1 Select and confirm Enter formula. Edit method 16.04.07 9:52 Enter formula Input fields for the coefficients (a0 c = a0 + a1·A + a2·A + a3·A + a4·A + a5·A ... a5) of the formula are displayed.
  • Page 100 ® Operation Spectroquant Pharo 300 5 If necessary, enter intervals for up Edit method 16.04.07 9:52 to 4 timers. Timer 0 00:00:00 Timer 1 00:00:00 6 If necessary, enter the AQA2 tar- Timer 2 00:00:00 Timer 3 00:00:00 get value and AQA2 tolerance.

  • Page 101: Measuring The Absorbance / % Transmission

    ® Spectroquant Pharo 300 Operation Measuring the Absorbance / % Transmission 4.6.1 General information The absorbance or transmission respectively is measured without the use of any methods or profiles. All settings are configured during measurement. Measuring against The absorbance or transmission can alternatively be measured against the...
  • Page 102 ® Operation Spectroquant Pharo 300 Rectangular cell: Open the inner turn-up lid. Insert the rectangular cell verti- cally so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back.

  • Page 103: Measuring Against The Reference Absorbance

    ® Spectroquant Pharo 300 Operation 4.6.3 Measuring against the Reference absorbance Each time the photometer is switched on, the absorbance or transmission is measured against the absorbance of the zero adjustment as a basis. You can, however, also determine a Reference absorbance and use it as the basis.
  • Page 104 ® Operation Spectroquant Pharo 300 2 Insert the cell with the reference Reference absorbance 16.04.07 9:52 sample. The first single measurement for the Reference absorbance is car- To start measurement, insert cell or press <START/ENTER> ried out. The following data is displayed as...

  • Page 105: Special / Multi Wavelengths Methods

    Basic information on Special / Multi wavelengths measure- ments ® In the Special / Multi wavelengths mode of the Spectroquant Pharo 300, you can carry out measurements with special methods and functions. You can use the following functions for these methods: ...

  • Page 106: Programming / Modifying The Special / Multi Wavelengths Methods

    ® Operation Spectroquant Pharo 300 4.7.2 Programming / modifying the Special / Multi wavelengths meth- Note For multi wavelength methods, you can use the method numbers 2001 to 2050. All special methods can also be selected in the method list of the con- centration mode.
  • Page 107 ® Spectroquant Pharo 300 Operation Example: The chlorophyll determination is based on two measurements (before and Determination of after adding an acid) of the optical density (= absorbance) of the extract of an chlorophyll a aqueous sample at 665 nm.
  • Page 108 ® Operation Spectroquant Pharo 300 <HOME> Special / Multi wavelengths – [Setup] – Edit method 1 Enter the general method data Edit method 16.04.07 9:52 here. The next available method Number 2001 number is already entered as the Name Chlorophyll a Version number.
  • Page 109 ® Spectroquant Pharo 300 Operation Create all required procedure vari- Procedure variables 16.04.07 9:52 ables. 5 Create a procedure variable Procedure variables are variables whose current numerical required for the formula with [Add] values have to be entered during the course of the and enter a designation, e.g.
  • Page 110 ® Operation Spectroquant Pharo 300 9 Select and confirm the variable. Formula entry 16.04.07 9:52 The current version of the formula is displayed. Variables A(665 nm) K1 (V extract (ml)) K2 (V sample Back Operators Variables Next Back 10 Add an operator.
  • Page 111 ® Spectroquant Pharo 300 Operation 14 Using [Next], accept all entries on Formula entry 16.04.07 9:52 the page and switch to the next R = 29.6 * (A665nm - A665nm_2) page. If an error is in the formula, an error message appears.
  • Page 112 ® Operation Spectroquant Pharo 300 If the formula includes several Edit method 16.04.07 9:52 measurements at the same wave- Sequence Designation length (measurement sequence), Measurement Measurement you can assign names to the indi- vidual measurements of the sequence. 18 Enter the names for the individual measurements of a sequence.

  • Page 113: Selecting A Special / Multi Wavelengths Method113

    ® Spectroquant Pharo 300 Operation 4.7.3 Selecting a Special / Multi wavelengths method To select a method for Special / Multi wavelengths measurements, proceed as follows: <HOME> Special / Multi wavelengths – [Method list] The list of methods is displayed.

  • Page 114: Carrying Out Special / Multi Wavelengths

    ® Operation Spectroquant Pharo 300 4.7.4 Carrying out Special / Multi wavelengths measurements <HOME> Special / Multi wavelengths 1 Select the required method with Special / Multi wavelengths 16.04.07 9:52 [Method list] (see section 4.7.3). Please select method for measuring!
  • Page 115 ® Spectroquant Pharo 300 Operation The photometer is ready to mea- Special / Multi wavelengths 16.04.07 9:52 sure. Measurement 1 To start measurement, insert cell or press <START/ENTER> 2001:Chl a Chlorophyll a 10 mm Setup Citation form Unit Method list...

  • Page 116: Measurements

    ® Operation Spectroquant Pharo 300 An intermediary result is displayed Special / Multi wavelengths 16.04.07 9:52 if there are several measure- V extract (ml) 10 ml V sample (ml) 100 ml ments. Measurement 1 A(665 n) = 0.600 The photometer is ready for the Proceed with <START/ENTER>...

  • Page 117: Spectrum

    ® Spectroquant Pharo 300 Operation Spectrum 4.8.1 General information With the Spectrum function, the absorbance or Transmission in dependency of the wavelength is measured and recorded. The wavelength range can be freely selected within the measuring range of the photometer. The increment is 1 nm.

  • Page 118: Recording The Spectrum

    ® Operation Spectroquant Pharo 300 4.8.2 Recording the Spectrum <HOME> Spectrum Spectrum 16.04.07 9:52 A message containing operating instructions is displayed. Spectrum 1 Open the setting menu with You have to record a baseline first [Setup]. (<ZERO>). Adjustment of wavelength range under <General setup>.
  • Page 119 ® Spectroquant Pharo 300 Operation The photometer is ready to mea- Spectrum 16.04.07 9:52 sure after the baseline has been recorded. Spectrum Start measurement with <START/ENTER> Wavelength [nm] Setup Open Recording the spectrum: 6 Depending on the type, insert the...
  • Page 120 ® Operation Spectroquant Pharo 300 Cursor information The cursor appears at the abso- lute maximum of the spectrum. Spectrum 16.04.07 9:52 13 You have the following options: - Immediately edit the spectrum (see section 4.8.3) - With <PRINT>, you can output the spectrum to a connected printer as a graphic.

  • Page 121: Loading/Editing A Spectrum

    ® Spectroquant Pharo 300 Operation 4.8.3 Loading/editing a spectrum A spectrum can be edited immediately after measurement. Stored spectra can be loaded and edited as well. The following tools are available for editing:  Cursor function for incremental moving along the curve with indication of the x and y values ...
  • Page 122 ® Operation Spectroquant Pharo 300 Zoom 1 Press [Zoom]. Spectrum 16.04.07 9:52 The zoom window appears. The bottom left corner of the zoom win- dow is marked by a small black square. – You can return to the original view of the spectrum with [Orig- inal] at any time.
  • Page 123 ® Spectroquant Pharo 300 Operation  Extreme values (zoomed area) Highlights the extreme values (minimum and maximum values) of the dis- played spectrum.  Mark points Opens an edit mode where you can highlight individual points of the spec- trum.

  • Page 124: Saving / Exporting A Spectrum

    ® Operation Spectroquant Pharo 300 4.8.4 Saving / exporting a spectrum The saving of a spectrum saves both the edited and the original spectrum. Consequently, the original spectrum can be restored from each stored spec- trum. Saving 1 Record a spectrum (see section Spectrum 16.04.07 9:52...

  • Page 125: Kinetics

    ® Spectroquant Pharo 300 Operation Kinetics The Kinetics function enables the temporal tracing of the absorbance or transmission of a sample at a certain wavelength. The photometer automatically calculates the slope between two adjacent measuring points from the available measurement data.
  • Page 126 ® Operation Spectroquant Pharo 300 Input field Possible entries Scaling: Auto** During measurement, the instrument adjusts the axis scaling (minimum and maximum value of the axis) to the measured values. The entire curve is always visible. Scaling:Manual The axis scaling (minimum and maximum value of the axis) is set manually.
  • Page 127 ® Spectroquant Pharo 300 Operation Creating/editing a <HOME> profile Kinetics – [Setup] – Edit profile 1 Enter the data for the profile here. Edit profile (1 of 2) 16.04.07 9:52 The next available profile number Number 4001 is already entered as the number.

  • Page 128: Loading A Profile For Kinetics Recording

    ® Operation Spectroquant Pharo 300 4.9.2 Loading a profile for Kinetics recording To load a profile for Kinetics recording, proceed as follows: <HOME> Kinetics – [Profile list] The list of profiles is displayed. Select profile (all) 16.04.07 9:52 The profiles are ordered according to the profile number.

  • Page 129: Recording The Kinetics

    ® Spectroquant Pharo 300 Operation 4.9.3 Recording the Kinetics Note During the recording, the photometer cannot carry out any regular self-test or self-calibration (AutoCheck), because the recording would have to be inter- rupted for this. A warm-up time of at least two hours is required for the pho- tometer to measure reliably during the recording.
  • Page 130 ® Operation Spectroquant Pharo 300 Rectangular cell: Open the inner turn-up lid. Insert the rectangular cell verti- cally so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back.
  • Page 131 ® Spectroquant Pharo 300 Operation Cursor information 4 After the specified Duration has expired, the cursor appears. Kinetics 16.04.07 9:52 You have the following options: 4002 Number of measurements: 4 Duration: 00:00:24 Interval:00:00:06 - You can move the cursor along...

  • Page 132: Saving / Exporting A Kinetics Record

    ® Operation Spectroquant Pharo 300 4.9.4 Saving / exporting a Kinetics record Saving 1 Carry out the kinetic recording Kinetics 16.04.07 9:52 (see section 4.9.3) 4002 Number of measurements: 4 Duration: 00:00:24 Interval:00:00:06 Load a stored kinetic record (see section 4.9.4).
  • Page 133 ® Spectroquant Pharo 300 Operation Example of a kinetic recording 6|4001|1|1|525|1280913092|59|5|1|0.000|0.301|0|1.000|µkat|2 (*.csv file) Device: Serial number:Software: User: Pharo 300 Merck 09130512 1.30- -1.60 Administrator Start time Wavelength [nm] 04.08.2010 11:11 Time [s] Absorbance 0,092 0,077 0,073 0,069 ..Line 1 - explanations:...

  • Page 134: Loading A Kinetics Record

    ® Operation Spectroquant Pharo 300 4.9.5 Loading a Kinetics record You can load and view stored Kinetics records. Loading a stored <HOME> Kinetics record Kinetics – [Open] The list with the stored Kinetics 16.04.07 9:52 records is displayed (Internal DataB folder).
  • Page 135 ® Spectroquant Pharo 300 Operation  Combined display of two kinetic records in one graphic  Display of the difference of two kinetic records Cursor Cursor information The cursor consists of a horizontal and vertical line that cross each other on a point of the curve. A box Kinetics 16.04.07 9:52...
  • Page 136 ® Operation Spectroquant Pharo 300 Measurements/interval Slope 1/interval Slope, converted to the interval, "1 minute" Max/interval Slope of the straight line determined by linear regression in an interval, converted to the inter- val, "1 minute" Scaling of the Y-axis You can manually determine the scaling of the Y-axis with [Setup]/Scaling/ Manual.

  • Page 137: Timer

    ® Spectroquant Pharo 300 Operation 4.10 Timer You can use the timers to remind you by an acoustic signal of a time interval that has expired. The photometer has two types of timers:  The User defined timer is a timer that can be freely assigned. The interval and name can be freely set.

  • Page 138: User Defined Timer

    ® Operation Spectroquant Pharo 300 4.10.1 User defined timer If you want to manually enter time intervals, use the User defined timer func- tion. <TIMER> The Timer menu is open. Timer 16.04.07 9:52 1 Highlight the User defined timer. Designation...
  • Page 139 ® Spectroquant Pharo 300 Operation 1 Select the required method in the Concentration mode. Manual selection of the method (see section 4.5.5). 2 Open the Timer menu. <TIMER> The Timer menu is open. Timer 16.04.07 9:52 3 If necessary, add a new timer to...

  • Page 140: Memory

    ® Operation Spectroquant Pharo 300 4.11 Memory 4.11.1 Overview AQA records Methods Concentration Spectrum Absorbance / % Transmission Profiles Kinetics Multi wavelengths <STORE> <STORE> AutoStore <STORE> Measured value memory <STORE> (Filter optional) Open in source application *.csv Internal folder *.csv...
  • Page 141 ® Spectroquant Pharo 300 Operation Measurement data Save, back up, export Concentration, Measurement datasets of these measuring modes are first stored in the measured value Absorbance / % Transmis- memory of the photometer (1000 memory sion locations) with <STORE> or AutoStore.

  • Page 142: Instructions On Using Usb Memory Devices

    ® Operation Spectroquant Pharo 300 4.11.2 Instructions on using USB memory devices The safety of data stored on USB memory devices depends on the quality of the memory device and the data transmission. Data is stored partly or not at all if for example: ...

  • Page 143: Measurement Datasets

    ® Spectroquant Pharo 300 Operation 4.11.3 Measurement datasets Elements of a A complete measurement dataset consists of: measurement  Consecutive number (is automatically assigned by the photometer) dataset  Date/time  Identification (e.g. ID or "AutoStore")  User name  Measured parameter, e.g. method number, dilution, wavelength (depend- ing on the measuring mode) ...
  • Page 144 ® Operation Spectroquant Pharo 300 automatically (see section 4.11.5) or manually (with the <STORE> key, see section 4.11.4):  Concentration  Multi wavelengths  Absorbance / % Transmission The data stored in the measured value memory can be filtered with filter cri- teria and then exported to the PC-readable *.csv format.

  • Page 145: Saving Measurement Datasets Manually

    ® Spectroquant Pharo 300 Operation 4.11.5 Saving measurement datasets automatically For the measuring modes, Concentration, Absorbance / % Transmission, and Special / Multi wavelengths you can record every measured value auto- matically (AutoStore). All automatically stored measurement datasets are given the ID "AutoStore".

  • Page 146: Displaying Measurement Data Memory

    ® Operation Spectroquant Pharo 300 4.11.6 Displaying measurement data memory Depending on the operating situation, you can recall the measured value memory as follows: From the main menu <HOME> [Setup], – Measurement data memory In Concentration Concentration mode – [Setup] –...
  • Page 147 ® Spectroquant Pharo 300 Operation  displayed in short form as a list or in details as individual values ([List] <–> [Single value])  filtered (see section 4.11.7 and section 4.11.8)  deleted (see section 4.11.9).  with <STORE>, you can store the entire displayed list as a *.csv file in the internal DataB folder or on a USB memory device connected to the USB- A connection.

  • Page 148: Filtering Measurement Datasets

    ® Operation Spectroquant Pharo 300 4.11.7 Filtering measurement datasets The functions to display, delete and download stored measurement datasets refer to all stored measurement datasets that correspond to the specified fil- ter criteria. Filter criteria The following filter criteria can be set: ...

  • Page 149: Inverting Filters

    ® Spectroquant Pharo 300 Operation Note Alternatively, you can hide measurement datasets that meet the specified fil- ter criteria with the Selected values: invert selection function (see section 4.11.8). 4.11.8 Inverting filters With the Selected values: invert selection function you can hide all measure- ment datasets that correspond to the specified criteria of the filter (see sec- tion 4.11.7).

  • Page 150: Erasing Stored Measurement Datasets

    ® Operation Spectroquant Pharo 300 4.11.9 Erasing stored measurement datasets If you no longer need any stored measurement datasets, you can erase them individually or altogether. <HOME> Concentration, Absorbance / % Transmission, or Special / Multi wavelengths – [Setup] – Measurement data...

  • Page 151: Saving Kinetic Recordings, Spectra And Aqa Files

    ® Spectroquant Pharo 300 Operation 4.11.10 Saving kinetic recordings, spectra and AQA files After the following measurements, the Save dialog opens and prompts you to save the data in a *.csv file:  Kinetics  Spectrum  AQA3/MatrixCheck If the data are not saved in *.csv format, they are lost when the measuring mode is terminated.

  • Page 152: Saving / Exporting Files

    ® Operation Spectroquant Pharo 300 4.12 Saving / exporting files If you want to back up or process measurement data files outside the pho- tometer, you can copy them to external media. Note Please follow the instructions on using USB memory devices (see section 4.11.2).

  • Page 153: Usb Memory Device

    ® Spectroquant Pharo 300 Operation 4.12.2 Copying user-defined methods / profiles to a USB memory device <HOME> [Setup] – Exchange methods/profiles /Store to USB memory device A list is displayed that includes all user-defined methods and profiles available on the photometer. All methods and profiles are checked off with a checkmark.

  • Page 154: Copying Files To A Pc

    ® Operation Spectroquant Pharo 300 4.12.3 Copying files to a PC You can copy from the photometer to a PC the following data:  Measurement data  Spectra  Kinetic recordings  AQA records  User-defined methods  Profiles After saving measurement data in *.csv or *.pdf format, you can copy them to a PC.

  • Page 155: Importing Files

    You can import to a Spectroquant Pharospectrophotometer the data that ® were created with the same or another Spectroquant Pharo spectropho- tometer, and the data that were saved to a USB memory device or a PC. You can import the following data: ...

  • Page 156: Importing Files From A Pc

    ® Operation Spectroquant Pharo 300 1 If necessary, select individual methods/profiles with <▲><▼> and remove the checkmark with<START ENTER>. These methods / profiles are excluded from importing. 2 Start the import with [Import]. A confirmation prompt appears before any data on the photometer are overwritten.

  • Page 157: Printing Data (Rs232, Usb)

    ® Spectroquant Pharo 300 Operation 4.14 Printing data (RS232, USB) 4.14.1 Printer and terminal programs Usable printers Data can be printed with the following printers:  Matrix printer connected to the RS232 interface  Standard printer (ink or laser) connected to the USB-A interface Note Suitable are all printers that can interpret the PCL-3 printer control language.

  • Page 158: Settings For Data Transmission

    ® RS232 interface face. Adjust the Spectroquant Pharo 300 to the baud rate of the printer. The setting is made in the following menu: <HOME> -> General setup -> Data transfer/Printer -> Baudrate for RS232 printer -> 1200 ... 19200.

  • Page 159: Printing Measurement Datasets

    ® Spectroquant Pharo 300 Operation 4.14.3 Printing measurement datasets This section describes how to print measurement datasets of the measuring modes, Concentration, Absorbance / % Transmission, and Special / Multi wavelengths. By means of sample printouts, the printed information is described below: Concentration 21 05.06.07 14:05:41 14541 844 mg/l COD...

  • Page 160: Printing Kinetics Records

    ® Operation Spectroquant Pharo 300 4.14.4 Printing Kinetics records Sample printout Pharo 300 Merck 09130512 1.30- -1.60 Administrator 05.06.07 12:14:55 320 nm Time [s] Absorbance 0,092 0,077 0,073 0,077 ..(etc.) Structure of the 1st line: lines from left to...

  • Page 161: Printing Spectra

    ® Spectroquant Pharo 300 Operation 4.14.5 Printing spectra Sample printout Pharo 300 Merck 09130512 1.30- -1.60 Administrator 07.06.07 09:47:00 Wavelength [nm] Absorbance 0,238 0,240 0,241 0,240 0,239 ..(etc.) Structure of the 1st line: lines from left to [Instrument type] [Series number] [Version of meter software and method...

  • Page 162: Analytical Quality Assurance (Aqa)

    ® Operation Spectroquant Pharo 300 4.15 Analytical quality assurance (AQA) 4.15.1 General information The target of the analytical quality assurance (AQA) is to secure correct and precise measurement results. Note Settings for AQA checks are only available for users of the user group, administrator.

  • Page 163: Photometer Monitoring (Aqa1)

    Spectroquant Pharo 300 Operation 4.15.2 Photometer monitoring (AQA1) ® At least one set of test standards such as Spectroquant PhotoCheck or Cer- ® tiPUR is required for the photometer monitoring. The administrator specifies which test standard has to be used as the mini- mum requirement for the AQA1 monitoring.
  • Page 164 ® Operation Spectroquant Pharo 300 Activating AQA1 The AQA1 monitoring is activated in the Mode menu: <HOME> [AQA] – AQA1 setup – Mode Select and confirm Weeks. AQA1 setup 16.04.07 9:52 AQA1 is active. Mode Weeks Interval The Interval setting indicates...
  • Page 165 ® Spectroquant Pharo 300 Operation <HOME> [AQA] – AQA1 setup – Lock instrument 1 Select and confirm Yes. AQA1 setup 16.04.07 9:52 The photometer is locked against Mode Weeks Interval all measurements if the AQA1 Lock instrument Lock instrument check is invalid or the AQA1 inter- Should the instrument be locked for further Configuration of tests ...
  • Page 166 ® Operation Spectroquant Pharo 300 Example, PhotoCheck: PhotoCheck 16.04.07 9:52 5 Using <▲><▼> and <><>, Lot number: HC616115 Use by 16.04.2008 select the Lot number, Target Target value Tolerance value or Tolerance entries and 445/1 0.196 ± 0.020 445/2 0.500 ±...
  • Page 167 ® Spectroquant Pharo 300 Operation 2 Insert the cell. PhotoCheck 445/1 16.04.07 9:52 The cuvette is automatically rec- ognized and the measurement started. Please insert PhotoCheck 445/1 After measuring, the measure- ment result, Target value, Toler- ance and an evaluation (OK or failed) are displayed.

  • Page 168: Total System Monitoring (Aqa2)

    Pharo 300 4.15.3 Total system monitoring (AQA2) For the total system monitoring, standard solutions with a defined analyte ® content are required (preferably certified Spectroquant CombiCheck stan- dards). Note Settings for AQA checks are only available for users of the user group, administrator.
  • Page 169 ® Spectroquant Pharo 300 Operation General AQA2 <HOME> settings [AQA] – AQA2 setup 1 Select and confirm Mode. AQA2 setup 16.04.07 9:52 The Mode selection field pops up. Mode Weeks Lock methods 2 Select and confirm Weeks or Mea- Method...
  • Page 170 ® Operation Spectroquant Pharo 300 Activating AQA2 <HOME> monitoring for a [AQA] method – AQA2 setup – Method 1 Select a method (see section AQA2 setup 16.04.07 9:52 Method 51: 14558 4.5.3). AQA2 AQA2 active Interval 12 Weeks 2 Select and confirm AQA2.
  • Page 171 ® Spectroquant Pharo 300 Operation Carrying out the AQA2 check for a <HOME> method [AQA] – AQA2 check 1 Carry out the check like a normal AQA2 check 16.04.07 9:52 measurement (see section 4.5.1 to 4.5.3). Target value 2.00 2 Insert the cell or To start measurement, insert cell or press <START/ENTER>...

  • Page 172: Aqa3/Matrixcheck

    ® Operation Spectroquant Pharo 300 4.15.4 AQA3/MatrixCheck The MatrixCheck is used to check if the photometric determination is dis- turbed by other substances present in the sample (sample matrix). The MatrixCheck can be carried out by spiking or diluting: The photometer enables a simplified MatrixCheck with the aid of the Spectro- ®...
  • Page 173 ® Spectroquant Pharo 300 Operation  After evaluating the measured value of the sample the photometer Practical instructions suggests for the MatrixCheck to spike or dilute the sample and standard with suitable volumes. You can change the suggested values of the volumes for the sample and standard.
  • Page 174 ® Operation Spectroquant Pharo 300 1 Enter and confirm a numerical AQA3/MatrixCheck setup 16.04.07 9:52 Maximum difference value. The setting is active. Maximum difference 2 Exit the menu with <ESC>. 10.0 Carrying out the 1 Measure the original sample with-...
  • Page 175 ® Spectroquant Pharo 300 Operation 7 In theStandard ID entry field, MatrixCheck (Spike) 16.04.07 9:52 select the simplified MatrixCheck Method 14: 14540 with the CombiCheck standard Sample concentration 45 mg/lCOD solution or enter a designation for Standard ID COD 1500...
  • Page 176 ® Operation Spectroquant Pharo 300 The measurement display MatrixCheck 16.04.07 9:52 appears. Method 14: 14540 Sample concentration 45 mg/lCOD 12 Insert the cell with the respective Sample 10 ml Standard 0.5 ml sample. The sample is measured. To start measurement, insert cell or press <START/ENTER>...
  • Page 177 ® Spectroquant Pharo 300 Operation The display returns to the mea- Concentration 16.04.07 9:52 sured value display of the original [MC] sample without spiking / dilution. The [MC] status indicator is displayed. A MatrixCheck was mg/l carried out for this measured value.

  • Page 178: User Management

    4.16.1 User levels and user rights ® The Spectroquant Pharo 300 allows the management of up to 100 users. Every user is member of a user group with defined user rights. User groups There are three hierarchical user groups:  Administrator (top level) ...

  • Page 179: Activating Or Deactivating The User Management Function

    ® Spectroquant Pharo 300 Operation To do so, you need administrator rights. If the user management is switched off, the user name and password do not have to be entered. Each user has full rights. 4.16.2 Activating or deactivating the User management function Each user can activate the user management function.

  • Page 180: Creating, Changing Or Deleting A User Account

    ® Operation Spectroquant Pharo 300 4.16.3 Creating, changing or deleting a user account When the user management function is active, a user with administrator rights can administrate user accounts. Creating a user During the creation of a user account, the Name, whether or not the user account belongs to a User group and the Password are defined.
  • Page 181 ® Spectroquant Pharo 300 Operation 1 Select a user account. User management 16.04.07 9:52 Name User group 2 Press [Change] to edit the user Administrator Administrator account. Admin2 Administrator User group The selection field for the user User Administrator group (Administrator / User) pops 3 If necessary, select and confirm another user group.

  • Page 182: Login With Active User Management

    The user management function is not active in the delivery condition of the ® Spectroquant Pharo 300. Every user can carry out all functions. Activating the user management creates an administrator user account. The user name is "Administrator". The preset password is "admin".
  • Page 183 ® Spectroquant Pharo 300 Operation The photometer is switched on. The Login dialog is displayed. 1 Enter the user name (<A...9>) and Login 16.04.07 9:52 confirm. The input field for the password pops up. Enter user name Administrator If the user name is not known (or...

  • Page 184: Changing The Password

    ® Operation Spectroquant Pharo 300 4.16.5 Changing the password The administrator sets up user accounts and assigns a password to each user account. As soon as any user has successfully logged in with the password, they can change the password for their user accounts themselves.

  • Page 185: Reset

    ® Spectroquant Pharo 300 Operation 4.17 Reset You can reset (initialize) the measurement settings or all settings. Note The Reset function is available for users of the user group, Administrator only. You have the following options of resetting the photometer settings: ...

  • Page 186: Photometer Information ([Info])

    ® Operation Spectroquant Pharo 300 4.18 Photometer information ([Info]) The following photometer information is displayed:  Photometer designation  Version number of the meter software/method data  Hardware version  Series number of the meter  Registered user  Hardware status (for service purposes) ...

  • Page 187: Software And Methods Update

    ® Spectroquant Pharo 300 Operation 4.20 Software and methods update The software and method update is used to continuously update your pho- tometer. Note Only members of the user group, Administrator may carry out any software and method updates. The update comprises ...
  • Page 188 ® Operation Spectroquant Pharo 300 Note Make sure the folder structure of the files is retained during the unpacking process. If you use a program such as WinZip to unpack the files, the option, "Nutze Ordnernamen" or "Use Folder Names" must be set. Details are given in the documentation of your unpack program.

  • Page 189: Update Using A Pc

    ® Spectroquant Pharo 300 Operation 4.20.2 Update using a PC The SpectralTransfer PC software supports the software and method update to Version 1.1x. From version 1.20 this function is no longer supported. 4.20.3 Language update If you want to set some special languages on your photometer (e.g. Chinese or Thai), a character set extension is required to display the characters.
  • Page 190 ® Operation Spectroquant Pharo 300 ba75703d07 04/2014...

  • Page 191: Maintenance And Cleaning

    ® Spectroquant Pharo 300 Maintenance and cleaning Maintenance and cleaning Exchanging the buffer batteries CAUTION There is a risk of explosion if unsuitable batteries are used. Only use leakproof alkaline manganese batteries. Note If you leave the photometer switched on during the exchange or insert the new batteries within a minute after taking out the old ones, the date and time are retained in the photometer.

  • Page 192: Cleaning

    ® Maintenance and cleaning Spectroquant Pharo 300 Cleaning Especially after a cell has broken or after a reagents accident, the photometer should immediately be cleaned (see also section 6.1 A CTIONS IN THE CASE OF A BROKEN CELL 5.2.1 Cleaning the enclosure...

  • Page 193: Cleaning The Detector Lens

    ® Spectroquant Pharo 300 Maintenance and cleaning 5.2.3 Cleaning the detector lens Normally, it is not required to clean the detector lens routinely. Cleaning the detector lens can be necessary in the following cases:  If the lens is visibly smudged, e.g. after a cell has broken or after a reagent accident (see also section 6.1 A...
  • Page 194 ® Maintenance and cleaning Spectroquant Pharo 300 ba75703e07 04/2014...

  • Page 195: What To Do If

    ® Spectroquant Pharo 300 What to do if ... What to do if ... Actions in the case of a broken cell WARNING Cells can contain dangerous substances. If the contents are released, follow the safety instructions of the package insert. If necessary, take corresponding protective measures (protective goggles, protective gloves etc.).

  • Page 196: Error Causes And Remedies

    ® What to do if ... Spectroquant Pharo 300 Cleaning the The detector lens is on the front left detector lens side of the rectangular cell shaft (pos. 1). 1 Switch off the photometer. 2 Cut off the end (approx. 2 cm) of a ®...
  • Page 197 ® Spectroquant Pharo 300 What to do if ... Self-test does not Cause Remedy start. – A cell is inserted in one of the cell – Remove the cell The instrument shafts displays – Then press the Please remove cell <START ENTER>...
  • Page 198 ® What to do if ... Spectroquant Pharo 300 Self test failed. Cause Remedy – System test: Instrument defective – Return instrument to service department – Filter test: Instrument defective – Return instrument to service department – Wavelength calibration: – Foreign particle in the cell shaft –...

  • Page 199: Technical Data

    ® Spectroquant Pharo 300 Technical data Technical data Measurement characteristics Measuring principle Single-beam spectrophotometer Light source Lamp type Xenon flashlamp Average lifetime 5 x 10 flashes, corresponding to at least 13000 h in permanent operation Monochromator Type Grating monochromator with step motor...
  • Page 200 Rectangular cell, 10 mm: 2 ml Rectangular cell, 20 mm: 4 ml Rectangular cell, 50 mm: 10 ml ® Cell recognition automatic for all Spectroquant cell tests and reagent tests Warm-up time At least 15 min for single measurements 2 h for kinetic measurements with the highest possible precision...
  • Page 201 ® Spectroquant Pharo 300 Technical data  Concentration Measuring modes – Measurement with permanently programmed methods, adjusted to the ® Spectroquant test set program – Automatic method selection if test sets with barcodes are used – Program support for the creation of additional user-defined methods (max.

  • Page 202: Measured Value Documentation And Quality Assurance

    ® Technical data Spectroquant Pharo 300 Measured value documentation and quality assurance Memory for Memory capacity – 1000 single measured values from the measured values measuring modes, concentration, absor- bance / % transmission and multi wave- lengths – 4 MByte internal memory, sufficient for approx.

  • Page 203: General Meter Data

    ® Spectroquant Pharo 300 Technical data General meter data Dimensions 404 x 197 x 314 mm (width x height x depth) Weight approx. 4.5 kg (without plug-in power supply) Housing type of IP 30 protection Electrical protective class Test mark...
  • Page 204 ® Technical data Spectroquant Pharo 300  German (Englisch) Available languages  English  Français  Español  Italiano  Bulgarian/Български  Česko  Simplified Chinese/ 中文 *  Traditional Chinese/ 繁體中文 *  Greek/Ελληνικά  Indonesian/Indonesia  Japanese/ 日本語...
  • Page 205 ® Spectroquant Pharo 300 Technical data FCC Class A Equipment Statement Note: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.
  • Page 206 ® Technical data Spectroquant Pharo 300 ba75703e07 04/2014...

  • Page 207: Accessories And Options

    Rectangular cell, quartz, 10 mm (1 pack = 2 cells) 1.00784.0001 Half micro cell, 50 mm (1 pack = 2 cells) 1.73502.0001 ® Positioning Aid for 10 mm plastic cells Spectroquant 1.00787.0001 Case and cable for Description Order no. mobile use ®...

  • Page 208: Test Equipment

    ® Accessories and options Spectroquant Pharo 300 Test equipment Test equipment for Description Order no. instrument check ® Spectroquant PhotoCheck 1.14693.0001 (AQA1) ® CertiPUR UV/VIS-Standard 1 - potassium dichromate 1.08160.0001 solution to check the absorption according to DAB and Ph.Eur.

  • Page 209: Connection Cable

    – Zero modem cable 9-pin (D-sub socket) - 9-pin Specialist (D-sub socket) shops ® USB printer You can connect a USB printer to the Spectroquant Pharo 300: Description Order no. – Cable with USB-B and USB-A plug Specialist shops Needle printer Suitable printers and cables are available on request.
  • Page 210 ® Accessories and options Spectroquant Pharo 300 ba75703e07 04/2014...

  • Page 211: Appendix

    ® Spectroquant Pharo 300 Appendix Appendix Menus Measuring (see section A.1.1) General settings and functions (see section A.1.2) A.1.1 Measuring  Concentration (see section A.1.1.1)  Absorbance / % Transmission (see section A.1.1.2)  Special / Multi wavelengths  Spectrum ...
  • Page 212 ® Appendix Spectroquant Pharo 300 Concentration – [Setup] – Edit method – [Next] – [Next] measure value pairs (Target value, Absorbance) – [Next] [Curve type] (Polygon line, Straight line, Parabola) [Meas. range] (Lower limit, Upper limit) – Enter formula – a5 ... a0, Meas. range (Lower limit, Upper limit) –...
  • Page 213 ® Spectroquant Pharo 300 Appendix A.1.1.2 Absorbance / % Transmission Absorbance / % Transmission – [Setup] – AQA (see section A.1.2.2) – Measurement data memory (see section A.1.2.1) – [Wavelength] – Set new wavelength (nm) – [Transmission] <–> [Absorbance] – [Reference] –...
  • Page 214 ® Appendix Spectroquant Pharo 300 A.1.1.4 Spectrum Spectrum – [Setup] – Wavelength start – Wavelength stop – Mode – Absorbance – Transmission – Smoothing – Yes – No – Scaling – Auto – Manual – Y-axis min – Y-axis max –...

  • Page 215: General Settings And Functions

    ® Spectroquant Pharo 300 Appendix A.1.1.5 Kinetics Kinetics – [Setup] – New profile – Edit profile – Scaling – Auto – Manual – Y-axis min – Y-axis max – [Profile list] – [All profiles] <–> [Last used] – [Edit] – Slope & catalytic activity –...
  • Page 216 ® Appendix Spectroquant Pharo 300 A.1.2.1 General setup [General setup] – Language – Date/Time – Date – Time – Display settings – Contrast [%] – User management – [Setup] – Deactivate user management – Change password – [Add] – [Delete] –...
  • Page 217 ® Spectroquant Pharo 300 Appendix [General setup] – Software/methods update – USB memory device – PC – Cancel – Reset – Reset configuration – Delivery condition – Data transfer/Printer – Decimal separator for csv-Files – Point (12.34) – Comma (12,34) –...
  • Page 218 ® Appendix Spectroquant Pharo 300 A.1.2.2 AQA [AQA] – AQA1 setup – Mode – AQA1 inactive – Weeks – Interval – Lock instrument (No/Yes) – Configuration of tests ... – PhotoCheck – CertiPUR UV-VIS 1 ... – AQA2 setup – Mode –...

  • Page 219: Glossary

    ® Spectroquant Pharo 300 Appendix Glossary Absorbance Logarithmic dimension for the absorption of the sample; negative decadal logarithm of the transmission. Analysis instructions The exact proceeding to carry out the detection procedure is described in the analysis instructions. Analytical Quality Assurance.
  • Page 220 How to carry out the method up to measuring with the photometer is described in the analysis instructions. ® The Spectroquant Pharo 300 contains a database with methods. Furthermore, user-defined methods can be entered in the database as well. PhotoCheck standard Stable color solution with defined absorbance values for the check of the photometer.

  • Page 221: List Of Trademarks

    ® Spectroquant Pharo 300 Appendix Test sample Designation of the test sample ready to be measured. Normally, a test sample is made by processing the original sample. The test sample and original sample are identical if the test sample was not pro- cessed.
  • Page 222 ® Appendix Spectroquant Pharo 300 ba75703e07 04/2014...

  • Page 223: Index

    ® Spectroquant Pharo 300 Index Absorbance / % Transmission, measuring ..........91 Accessories .....................207 Analysis timer ..................138 Analytical quality assurance (AQA) ............162 AQA ......................162 Authorized use ..................34 AutoCheck ....................51 Barcode .....................66 Barcode reader ..................46 Blank value Reagent blank value ................79 Sample blank value ................77 Cell breakage ..................195...
  • Page 224 ® Spectroquant Pharo 300 Meter information ..................186 Method ....................113 Methods update ..................187 Multi-wavelengths methods ..............105 Operating elements .................. 29 Operating principles .................. 52 Operational safety ..................34 Overview ....................29 Print ......................157 Printer ..................... 157 Profile (kinetic) ..................128 Profile (spectrum) ...................
  • Page 225 ® Spectroquant Pharo 300 Update ....................187 USB memory device .................45 User calibration ..................83 User-defined methods Concentration ..................91 Multi wavelengths ................106 Warm-up time ...................50 Zero adjustment ..................61 ba75703e07 04/2014...
  • Page 226 ® Spectroquant Pharo 300 ba75703e07 04/2014...
  • Page 227 Spectroquant UV/VIS Spectrophotometer ® Pharo 300 Analytical Procedures Appendices...
  • Page 228 Spectroquant ® photometers Contents Available photometric test kits Table – Analytical Procedures Suitability of Test Kits for Testing Seawater Appendix 1 – and Tolerance Limits of Neutral Salts ® Spectroquant CombiCheck and Standard Appendix 2 – Solutions Instructions for the Preparation of Standard Appendix 3 –...
  • Page 229 Spectroquant ® UV/VIS Spectrophotometer Pharo 300 Available photometric test kits The following methods with the corresponding method numbers are programmed into the photometer and mea- surements can be made without any further adjustments. Method selection is achieved through a barcode on the cell (for cell tests) or through a barcode on the AutoSelector (for reagent tests).
  • Page 230 Spectroquant ® UV/VIS Spectrophotometer Pharo 300 Available photometric test kits Method Determination Total Range Method COD Cell Test* 101796 5.0 – 80.0 mg/l COD Chromosulfuric acid oxidation, chromate determination COD Cell Test* 114540 10 – 150 mg/l COD Chromosulfuric acid oxidation,...
  • Page 231 Spectroquant ® UV/VIS Spectrophotometer Pharo 300 Available photometric test kits Method Determination Total Range Method Hydrogenperoxide Cell Test sens.* 114731 0.25 – 5.00 mg/l H Titanyl sulfate Hydrogenperoxide Test 118789 0.015 – 6.00 mg/l H Phenanthroline derivative Iodine Test* 100606 0.050 –...
  • Page 232 Spectroquant ® UV/VIS Spectrophotometer Pharo 300 Available photometric test kits Method Determination Total Range Method Potassium Cell Test 114562 5.0 – 50.0 mg/l K Kalignost, turbidimetric Potassium Cell Test 100615 30 – 300 mg/l K Kalignost, turbidimetric Residual Hardness Cell Test* 114683 0.50 –...
  • Page 233 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a sodium hydroxide solution 0.1 mol/l, Cat.No. 109141, can be used after di- luting accordingly (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 234 To check the measurement system (measurement device, and handling) ready-for-use platinum-cobalt color refer- ® ence solution (Hazen 500) Certipur , Cat.No. 100246, concentration 500 mg/l Pt can be used after diluting ac- cordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 235 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® aluminium standard solution Certipur , Cat.No. 119770, concentration 1000 mg/l Al can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 236 Ready-for-use aluminium standard solution Certipur Cat.No. 119770, concentration 1000 mg/l Al, can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 40) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 237 (plausibility check). To measure in the 50-mm cell, the sample volume and the volume of the reagents have to be doubled for each. Alternatively, the semi-microcell, Cat.No. 173502, can be used. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 238 Cat.No. 119812, concentration 1000 mg/l NH , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 50) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 239 Cat.No. 119812, concentration 1000 mg/l NH , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 10) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 240 Cat.No. 119812, concentration 1000 mg/l NH , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 20) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 241 Cat.No. 119812, concentration 1000 mg/l NH , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 70) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 242 , can also Alternatively, the semi-microcell, Cat.No. 173502, can be be used after diluting accordingly. used. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 50) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 243 To check for sample-dependent effects the use of addition ings are yielded. In such cases the sample must be di- solutions (e.g. in CombiCheck 70) is highly recommended. luted (plausibility check). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 244 2, and 3 used are given in the corresponding application, sealed with the screw caps, thus enabling a hazard-free which also includes further information on the method mixing of the sample. employed. This application can be downloaded directly at www.analytical-test-kits.com. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 245 1-ml portions If required, add dilute bottom end). through completely. of AOX-3. Discard the sodium hydroxide wash solution. solution or nitric acid drop by drop to adjust the pH. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 246 Quality assurance: To check the measurement system (test reagents, ® measurement device, and handling) Spectroquant Standard, Cat.No. 100680, concentration 0.2 – 2.0 mg/l can be used. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 247 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® arsenic standard solution Certipur , Cat.No. 119773, concentration 1000 mg/l As can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 248 Align the mark on the cell with To check the measurement system (test reagents, that on the photometer. measurement device, and handling) Spectroquant BOD Standard (acc. to EN 1899), Cat.No. 100718, can be used. ® Release 06/2014 - Spectroquant...
  • Page 249 To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® boron standard solution Certipur , Cat.No. 119500, concentration 1000 mg/l B can also be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 250 ® boron standard solution Certipur , Cat.No. 119500, concentration 1000 mg/l B can also be used after diluting accordingly. Place the cell into the cell compartment. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 251 The exact composition and preparation of the reagents 1 and 2 used are given in the corresponding application, which also includes further information on the method employed. This application can be downloaded directly at www.analytical-test-kits.com. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 252 In such cases the standard solution can be used (see section “Standard sample must be diluted (plausibility check). solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 253 Ready-for-use cadmium standard solution Certipur Cat.No. 119777, concentration 1000 mg/l Cd, can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 30) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 254 Cat.No. 114687, and thermoreactor is necessary. cadmium standard solution Certipur , Cat.No. 119777, concentration 1000 mg/l Cd, can be used after diluting  Result can be expressed as sum of cadmium ( Cd). accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 255 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a freshly prepared standard solution can be used (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 256 To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® calcium standard solution Certipur , Cat.No. 119778, concentration 1000 mg/l Ca, can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 257 5 standard solutions over the entire measuring range. calcium standard solution Certipur , Cat.No. 119778, The calibration should be checked regularly using stan- concentration 1000 mg/l Ca, can be used after dard solutions. diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 258 – Cat.No. 119897, concentration 1000 mg/l Cl , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 259 Cat.No. 119897, concentration 1000 mg/l Cl , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 60) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 260 To check the measurement system (test reagents, meas- urement device, and handling) ready-for-use chloride ® standard solution Certipur , Cat.No. 119897, concentra- tion 1000 mg/l Cl , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 261 To check the measurement system (test reagents, meas- urement device, and handling) ready-for-use chloride ® standard solution Certipur , Cat.No. 119897, concentra- tion 1000 mg/l Cl , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 262 In such cases the standard solution can be used (see section “Standard sample must be diluted (plausibility check). solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 263 (see section “Standard sample must be diluted (plausibility check). solutions”). After each determination of total chlorine rinse the cell with sulfuric acid 25 % and subsequently several times with distilled water. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 264 In such cases the standard solution can be used (see section “Standard sample must be diluted (plausibility check). solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 265 Chloramine T GR can be used (see sample must be diluted (plausibility check). section “Standard solutions”). After each determination of total chlorine rinse the cell with sulfuric acid 25 % and subsequently several times with distilled water. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 266 (see section “Standard sample must be diluted (plausibility check). solutions”). After each determination of total chlorine rinse the cell with sulfuric acid 25 % and subsequently several times with distilled water. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 267 (see section “Standard sample must be diluted (plausibility check). solutions”). After each determination of total chlorine rinse the cell with sulfuric acid 25 % and subsequently several times with distilled water. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 268 (see section “Standard sample must be diluted (plausibility check). solutions”). After each determination of total chlorine rinse the cell with sulfuric acid 25 % and subsequently several times with distilled water. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 269 AutoSelector. cell compartment. substance. Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a freshly prepared standard solution can be used (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 270 A (before acid.) [Abs] (before acid.) [Abs] Select method no. 2509, Place the cell into the 2510, or 2511. cell compartment. Enter the volumes of the original sample and extract (volumetric flask). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 271 Phaeo [µg/l] Important: The exact procedure as well as further details on the method used can be found in the corresponding applica- tion. This application can be downloaded directly at www.analytical-test-kits.com. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 272 A (before acid.) [Abs] (before acid.) [Abs] Select method no. 2504, Place the cell into the 2505, or 2506. cell compartment. Enter the volumes of the original sample and extract (here: 10 ml). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 273 The exact procedure and the composition and preparation of the extraction agent used are given in the correspond- ing application, which also includes further information on the method employed. This application can be download- ed directly at www.analytical-test-kits.com. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 274 A (before acid.) [Abs] (before acid.) [Abs] Select method no. 2504, Place the cell into the 2505, or 2506. cell compartment. Enter the volumes of the original sample and extract (here: 10 ml). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 275 The exact procedure and the composition and preparation of the extraction agent used are given in the correspond- ing application, which also includes further information on the method employed. This application can be download- ed directly at www.analytical-test-kits.com. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 276 The exact procedure and the composition and preparation of the extraction agent used are given in the correspond- ing application, which also includes further information on the method employed. This application can be download- ed directly at www.analytical-test-kits.com. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 277 The exact procedure and the composition and preparation of the extraction agent used are given in the correspond- ing application, which also includes further information on the method employed. This application can be download- ed directly at www.analytical-test-kits.com. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 278 To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® chromate standard solution Certipur , Cat.No. 119780, 2– concen tration 1000 mg/l CrO , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 279 To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® chromate standard solution Certipur , Cat.No. 119780, 2– concen tration 1000 mg/l CrO , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 280 Result can be expressed as sum of chromium ( Cr). To measure in the 50-mm cell, the sample volume and the volume of the reagents have to be doubled for each. Alternatively, the semi-microcell, Cat.No. 173502, can be used. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 281 (Empty cells, Cat. fill to the mark with mix. distilled water and distilled water and mix No. 114724). mix thoroughly. thoroughly. Place the cell into the cell compartment. Select method no. 20. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 282 The exact composition and preparation of the reagents 1, 2, and 3 used are given in the corresponding application, which also includes further information on the method employed. This application can be downloaded directly at www.analytical-test-kits.com. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 283 CombiCheck 50, Cat.No. 114695, or the Standard solution for photometric applications, CRM, Cat.No. 125028. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 50) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 284 CombiCheck 50, Cat.No. 114695, or the Standard solution for photometric applications, CRM, Cat.No. 125028. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 50) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 285 CombiCheck 10, Cat.No. 114676, or the Standard solution for photometric applications, CRM, Cat.No. 125029. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 10) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 286 CombiCheck 60, Cat.No. 114696, or the Standard solution for photometric applications, CRM, Cat.No. 125029 and 125030. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 60) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 287 CombiCheck 60, Cat.No. 114696, or the Standard solution for photometric applications, CRM, Cat.No. 125029, 125030, and 125031. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 60) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 288 CombiCheck 20, Cat.No. 114675, or the Standard solution for photometric applications, CRM, Cat.No. 125029, 125030, 125031, and 125032. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 20) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 289 CombiCheck 80, Cat.No. 114738, or the Standard solution for photometric applications, CRM, Cat.No. 125031, 125032, and 125033. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 80) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 290 CombiCheck 70, Cat.No. 114689, or the Standard solution for photometric applications, CRM, Cat.No. 125032, 125033, and 125034. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 70) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 291 Quality assurance: To check the measurement system (test reagents, meas- urement device, and handling) we recommended to use the Standard solution for photometric applications, CRM, Cat.No. 125034 and 125035. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 292 Quality assurance: To check the measurement system (test reagents, meas- urement device, and handling) we recommended to use the Standard solution for photometric applications, CRM, Cat.No. 125028 and 125029. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 293 Quality assurance: To check the measurement system (test reagents, meas- urement device, and handling) we recommended to use the Standard solution for photometric applications, CRM, Cat.No. 125029, 125030, 125031, and 125032. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 294 Holding the reagent bottle vertically, slowly add reagent Cl-3 dropwise to the sample while swirling until its color changes from yellow to blue-violet. Shortly before the color changes, wait a few seconds after adding each drop. Result in mg/l chloride = number of drops x 250 ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 295 To check the measurement system (test reagents, meas- urement device, and handling) a COD/chloride standard solution must be prepared from Potassium hydrogen phthalate, Cat.No. 102400 and Sodium chloride, Cat.No. 106404 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 296 Holding the reagent bottle vertically, slowly add reagent Cl-3 dropwise to the sample while swirling until its color changes from yellow to blue-violet. Shortly before the color changes, wait a few seconds after adding each drop. Result in mg/l chloride = number of drops x 250 ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 297 To check the measurement system (test reagents, meas- urement device, and handling) a COD/chloride standard solution must be prepared from Potassium hydrogen phthalate, Cat.No. 102400 and Sodium chloride, Cat.No. 106404 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 298 0.45 µm pore cell. method no. 15, 61, or size. Notes: Filtered sample = true color. Unfiltered sample = apparent color. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 299 50-mm cell Filter sample solution Transfer the solution Place the cell into the through a membrane into a corresponding cell compartment, select filter with 0.45 µm pore cell. method no. 303. size. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 300 To check the measurement system (measurement device, handling) ready-for-use Platinum Cobalt Color Reference ® Solution (Hazen 500) Certipur , Cat.No. 100246, concentra- tion 500 mg/l Pt, can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 301 Quality assurance: To check the measurement system (measurement device, handling) ready-for-use Platinum Cobalt Color Reference ® Solution (Hazen 500) Certipur , Cat.No. 100246, concentra- tion 500 mg/l Pt, can be used. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 302 To check for sample-dependent effects the use of addition Cat.No. 114687 and thermoreactor is necessary. solutions (e.g. in CombiCheck 30) is highly recommended.  Result can be expressed as sum of copper ( Cu). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 303 Result can be expressed as sum of copper ( Cu). To measure in the 50-mm cell, only the sample volume has to be doubled. Alternatively, the semi-microcell, Cat.No. 173502, can be used. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 304 83. fill to the mark with mix. (Empty cells, Cat.No. distilled water and 114724). mix thoroughly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 305 To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® cyanide standard solution Certipur , Cat.No. 119533, – concentration 1000 mg/l CN , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 306 To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® cyanide standard solution Certipur , Cat.No. 119533, – concentration 1000 mg/l CN , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 307 To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® cyanide standard solution Certipur , Cat.No. 119533, – concentration 1000 mg/l CN , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 308 To measure in the 50-mm cell, the sample volume and the volume of the reagents CN-3 and CN-4 have to be doubled for each. Alternatively, the semi-microcell, Cat.No. 173502, can be used. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 309 To check the measurement system (test reagents, cell compartment. measurement device, and handling) ready-for-use ® cyanide standard solution Certipur , Cat.No. 119533, – concentration 1000 mg/l CN , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 310 Quality assurance: To check the measurement system (test reagents, meas- urement device, and handling) a cyanuric acid standard solution must be prepared from Cyanuric acid, Cat.No. 820358 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 311 In such cases fluoride standard solution Certipur , Cat.No. 119814, – the sample must be diluted (plausibility check). concentration 1000 mg/l F , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 312 In such cases fluoride standard solution Certipur , Cat.No. 119814, – the sample must be diluted (plausibility check). concentration 1000 mg/l F , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 313 In such cases fluoride standard solution Certipur , Cat.No. 119814, – the sample must be diluted (plausibility check). concentration 1000 mg/l F , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 314 In such cases fluoride standard solution Certipur , Cat.No. 119814, – the sample must be diluted (plausibility check). concentration 1000 mg/l F , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 315 ® doubled for each. fluoride standard solution Certipur , Cat.No. 119814, – concentration 1000 mg/l F , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 316 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a formaldehyde standard solution must be prepared from Formaldehyde solution 37%, Cat.No. 104003 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 317 Formaldehyde mixing of the sample. solution 37%, Cat.No. 104003 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 318 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® gold standard solution Certipur , Cat.No. 170216, concentration 1000 mg/l Au, can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 319 Alternatively, the semi-microcell, Cat.No. 173502, can be solution must be prepared from Hydrazinium sulfate GR, used. Cat.No. 104603 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 320 However, this does not influence the measurement result. measurement device, and handling) a hydrogenperoxide ® standard solution must be prepared from Perhydrol 30% H GR, Cat.No. 107209 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 321 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a hydrogenperoxide ® standard solution must be prepared from Perhydrol 30% H GR, Cat.No. 107209 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 322 In such cases the standard solution can be used (see section “Standard sample must be diluted (plausibility check). solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 323 340 nm 20-mm cell 0.010 – 0.600 340 nm 50-mm cell Filter turbid samples. Transfer the solution into Place the cell into the a corresponding cell. cell compartment, select method no. 33. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 324 445 nm 20-mm cell 0.2 – 10.0 445 nm 50-mm cell Filter turbid samples. Transfer the solution into Place the cell into the a corresponding cell. cell compartment, select method no. 21. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 325 Ready-for-use iron standard solution Certipur Cat.No. 119781, concentration 1000 mg/l Fe, can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 30) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 326 Cat.No. 114687, and thermoreactor is necessary. iron standard solution Certipur , Cat.No. 119687, concentration 1000 mg/l Fe(III), can be used after  Result can be expressed as sum of iron ( Fe). diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 327 To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 30) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 328 Ready-for-use iron standard solution Certipur Cat.No. 119781, concentration 1000 mg/l Fe(III), can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 30) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 329 Ready-for-use lead standard solution Certipur Cat.No. 119776, concentration 1000 mg/l Pb, can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 40) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 330 Ready-for-use lead standard solution Certipur Cat.No. 119776, concentration 1000 mg/l Pb, can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 40) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 331 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a freshly prepared standard solution can be used (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 332 Ready-for-use manganese standard solution Certipur Cat.No. 119789, concentration 1000 mg/l Mn, can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 30) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 333 ® measurement sample, but with distilled water instead of manganese standard solution Certipur , Cat.No. 119789, sample). concentration 1000 mg/l Mn, can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 334 Ready-for-use manganese standard solution Certipur Cat.No. 119789, concentration 1000 mg/l Mn, can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 30) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 335 ® measurement sample, but with distilled water instead of manganese standard solution Certipur , Cat.No. 119789, sample). concentration 1000 mg/l Mn, can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 336 The exact composition and preparation of the reagents 1 and 2 used are given in the corresponding application, which also includes further information on the method employed. This application can be downloaded directly at www.analytical-test-kits.com. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 337 To check the measurement system (test reagents, measurement device, and handling) a ready-for-use ® molybdenum standard solution Certipur , Cat.No. 170227, concentration 1000 mg/l Mo, can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 338 To check the measurement system (test reagents, measurement device, and handling) a ready-for-use ® molybdenum standard solution Certipur , Cat.No. 170227, concentration 1000 mg/l Mo, can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 339 (see section “Standard solutions”). readings are yielded. In such cases the sample must be diluted (plausibility check). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 340 A nickel standard solution Titrisol , Cat.No. 109989, can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 40) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 341 To check for sample-dependent effects the use of addition Alternatively, the semi-microcell, Cat.No. 173502, can be solutions (e.g. in CombiCheck 40) is highly recommended. used. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 342 40 %, close the cell a corresponding rectan- cell compartment. round cell (Empty cells, with the screw cap, and gular cell. Select method no. 57. Cat.No. 114724). mix. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 343 Cat.No. 119811, concentration 1000 mg/l NO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 20) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 344 Cat.No. 119811, concentration 1000 mg/l NO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 20) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 345 Cat.No. 119811, concentration 1000 mg/l NO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 80) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 346 Certipur , Cat.No. 119811, concentration – 1000 mg/l NO , can be used after diluting accordingly as well as the Standard solution for photometric applications, CRM, Cat.No. 125039 and 125040. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 347 – Cat.No. 119811, concentration 1000 mg/l NO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 348 These cells can be To check for sample-dependent effects the use of addition sealed with the screw caps, thus enabling a hazard-free solutions (e.g. in CombiCheck 20) is highly recommended. mixing of the sample. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 349 Cat.No. 119811, concentration 1000 mg/l NO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 10) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 350 Cat.No. 119811, concentration 1000 mg/l NO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 20) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 351 To check the measurement system (test reagents, measurement device, and handling) a ready-for-use ® nitrate standard solution Certipur , Cat.No. 119811, concentration 1000 mg/l NO , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 352 (matrix effect). method used can be found in the corresponding applica- In this case an evaluation is not possible. tion. This application can be downloaded directly at www.analytical-test-kits.com. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 353 ® dard solution Certipur , Cat.No. 119899, concentration – 1000 mg/l NO , can be used after diluting accordingly as well as the Standard solution for photometric applications, CRM, Cat.No. 125041. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 354 ® dard solution Certipur , Cat.No. 119899, concentration – 1000 mg/l NO , can be used after diluting accordingly as well as the Standard solution for photometric applications, CRM, Cat.No. 125042. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 355 Certipur , Cat.No. 119899, concentration – used. 1000 mg/l NO , can be used after diluting accordingly as well as the Standard solution for photometric applications, CRM, Cat.No. 125041. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 356 CombiCheck 50, Cat.No. 114695, or the Standard solution for photometric applications, CRM, Cat.No. 125043 and 125044. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 50) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 357 CombiCheck 50, Cat.No. 114695, or the Standard solution for photometric applications, CRM, Cat.No. 125043 and 125044. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 50) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 358 CombiCheck 70, Cat.No. 114689, or the Standard solution for photometric applications, CRM, Cat.No. 125044 and 125045. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 70) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 359 Quality assurance: To check the measurement system (test reagents, meas- urement device, and handling) a oxygen standard solution must be prepared (application see the website). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 360 Quality assurance: To check the measurement system (test reagents, meas- urement device, and handling) a oxygen scavengers stan- dard solution must be prepared from N,N-diethylhydroxyl- amine, Cat.No. 818473 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 361 In such cases the standard solution can be used (see section “Standard sample must be diluted (plausibility check). solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 362 2 used are given in the corresponding application, sealed with the screw caps, thus enabling a hazard-free which also includes further information on the method mixing of the sample. employed. This application can be downloaded directly at www.analytical-test-kits.com. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 363 ! Quality assurance: To check the measurement system (test reagents, measurement device, and handling) buffer solution ® pH 7.00 Certipur , Cat.No. 109407, can be used. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 364 In such cases the sample must be diluted solution must be prepared from Phenol GR, Cat.No. (plausibility check). 100206 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 365 5 – 10 minutes to allow phase into a cell. (protected from light) ration funnel. the phases to separate. Select method with Place the cell into the AutoSelector measuring cell compartment. range 0.002 – 0.100 mg/l. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 366 Phenol GR, Cat.No. 100673 or Phosphate Test, Cat. No. 114848 in conjunction 100206 (see section “Standard solutions”). with Crack Set 10/10C, Cat. No. 114687 resp. 114688 can be used. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 367 Cat.No. 119898, concentration 1000 mg/l PO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 10) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 368 Cat.No. 119898, concentration 1000 mg/l PO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 10) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 369 Cat.No. 119898, concentration 1000 mg/l PO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 10) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 370 3– Cat.No. 119898, concentration 1000 mg/l PO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 371 3– Cat.No. 119898, concentration 1000 mg/l PO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 372 3– Cat.No. 119898, concentration 1000 mg/l PO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 373 100673 or Phosphate Test, Cat. No. 114848 in conjunction concentration 1000 mg/l PO , can be used after with Crack Set 10/10C, Cat. No. 114687 resp. 114688 can diluting accordingly. be used. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 374 To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® phosphate standard solution Certipur , Cat.No. 119898, 3– concentration 1000 mg/l PO , can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 375 3– tion 1000 mg/l PO , can be used after diluting according- and organophosphate. ly as well as the Standard solution for photometric applica- tions, CRM, Cat.No. 125047, 125048, and 125049. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 376 114688, or Crack Set 10, Cat.No. 114687, and thermore- To check for sample-dependent effects the use of addition actor is necessary. solutions (e.g. in CombiCheck 10) is highly recommended.  Result can be expressed as sum of phosphorus ( ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 377 100673 or Phosphate Test, Cat. No. 114848 in conjunction concentration 1000 mg/l PO , can be used after with Crack Set 10/10C, Cat. No. 114687 resp. 114688 can diluting accordingly. be used. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 378 100673 or Phosphate Test, Cat. No. 114848 in conjunction concentration 1000 mg/l PO , can be used after with Crack Set 10/10C, Cat. No. 114687 resp. 114688 can diluting accordingly. be used. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 379 100673 or Phosphate Test, Cat. No. 114848 in conjunction concentration 1000 mg/l PO , can be used after with Crack Set 10/10C, Cat. No. 114687 resp. 114688 can diluting accordingly. be used. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 380 2 used are given in the corresponding application, sealed with the screw caps, thus enabling a hazard-free which also includes further information on the method mixing of the sample. employed. This application can be downloaded directly at www.analytical-test-kits.com. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 381 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® potassium standard solution Certipur , Cat.No. 170230, concentration 1000 mg/l K, can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 382 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® potassium standard solution Certipur , Cat.No. 170230, concentration 1000 mg/l K, can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 383 To check the measurement system (test reagents, measurement device, and handling) ready-for-use ® calcium standard solution Certipur , Cat.No. 119778, concentration 1000 mg/l Ca, can be used after diluting accordingly. (Pay attention to pH value! ) ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 384 Certipur , Cat.No. 170236, concentra- used. tion 1000 mg/l Si, can be used after diluting accordingly. (Atten tion! Do not store standard solutions in glass vessels - see section "Standard solutions"!) ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 385 Certipur , Cat.No. 170236, concentra- tion 1000 mg/l Si, can be used after diluting accordingly. (Atten tion! Do not store standard solutions in glass vessels - see section "Standard solutions"!) ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 386 Certipur , Cat.No. 170236, concentration 1000 mg/l Si, can be used after diluting accordingly (Atten tion! Do not store standard solutions in glass vessels - see section "Standard solutions"). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 387 To check the measurement system (test reagents, measurement device, and handling) ready- ® for-use silver standard solution Certipur , Cat.No. 119797, concentration 1000 mg/l Ag, can be Place the cell into the used after diluting accordingly. cell compartment. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 388 ® chloride standard solution Certipur , Cat.No. 119897, concentration 1000 mg/l Cl (corresponds to 649 mg/l Na), can be used after diluting accordingly (see section “Stan- dard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 389 50-mm cell Filter sample solution Transfer the solution Place the cell into the through a membrane into a corresponding cell compartment, select filter with 0.45 µm pore cell. method no. 300. size. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 390 When the turbidity correction function is activated (see Descrip- tion of Function, section 4.5.9 “Automatic Turbidity correction”), the corrected spectral attenuation coefficient (254)korr can be determined. The turbidity correction is carried out as per DIN 38404 at 550 nm. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 391 Place the cell into the through a membrane into a corresponding cell compartment, select filter with 0.45 µm pore cell. method no. 302. size. Notes: Filtered sample = true color. Unfiltered sample = apparent color. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 392 To check the measurement system (test reagents, meas- urement device, and handling) ready-for-use sulfate ® standard solution Certipur , Cat.No. 119813, concen- tration 1000 mg/l SO , can be used after diluting accord- ingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 393 Cat.No. 119813, concentration 1000 mg/l SO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 10) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 394 Cat.No. 119813, concentration 1000 mg/l SO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 10) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 395 Cat.No. 119813, concentration 1000 mg/l SO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 20) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 396 Cat.No. 119813, concentration 1000 mg/l SO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 10) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 397 To check the measurement system (test reagents, meas- urement device, and handling) ready-for-use sulfate ® standard solution Certipur , Cat.No. 119813, concen- tration 1000 mg/l SO , can be used after diluting accord- ingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 398 Cat.No. 119813, concentration 1000 mg/l SO , can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 10) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 399 Alternatively, the semi-microcell, Cat.No. 173502, can be solution must be prepared from sodium sulfide GR (see used. section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 400 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a sulfite standard solution must be prepared from sodium sulfite GR, Cat.No. 106657 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 401 (Blank) Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a sulfite standard solution must be prepared from sodium sulfite GR, Cat.No. 106657 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 402 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a sulfite standard solution must be prepared from sodium sulfite GR, Cat.No. 106657 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 403 To check the measurement system (test reagents, measurement device, and handling) a surfactants standard solution must be prepared from dodecane-1- sulfonic acid sodium salt GR, Cat.No. 112146 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 404 To check the measurement system (test reagents, measurement device, and handling) a surfactants standard solution must be prepared from dodecane-1- sulfonic acid sodium salt GR, Cat.No. 112146 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 405 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a surfactants standard solution must be prepared from N-cetyl-N,N,N- trimethylammonium bromide, Cat.No. 102342 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 406 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a surfactants ® standard solution must be prepared from Triton X-100, Cat.No. 112298 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 407 Homogenize 500 ml of Transfer the solution into Place the cell into the sample for 2 minutes in a a cell. cell compartment, select mixer running at high method no. 182. speed. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 408 To check the measurement system (test reagents, meas- urement device, and handling) a tin standard solution must be prepared from ready-for-use tin standard solution ® Certipur , Cat.No. 170242, concentration 1000 mg/l Sn (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 409 To check the measurement system (test reagents, measure ment device, and handling) a TOC standard ® solution Certipur , Cat.No. 109017, concentration 1000 mg/l TOC, can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 410 To check the measurement system (test reagents, measure ment device, and handling) a TOC standard ® solution Certipur , Cat.No. 109017, concentration 1000 mg/l TOC, can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 411 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a freshly prepared standard solution can be used (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 412 Align cell, close the cell with the mark on the cell with the screw cap, and mix. that on the photometer = Result magnesium = Result calcium ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 413 EN ISO 7027 Measuring range: 1 – 100 FAU 550 nm 50-mm cell Transfer the sample into Place the cell into the a cell. cell compartment, select method No. 177. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 414 To check the measurement system (test reagents, measurement device, and handling) a standard solution must be prepared from sodium acetate anhydrous, Cat.No. 106268 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 415 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a standard solution must be prepared from sodium acetate anhydrous, Cat.No. 106268 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 416 Quality assurance: To check the measurement system (test reagents, measurement device, and handling) a standard solution must be prepared from sodium acetate anhydrous, Cat.No. 106268 (see section “Standard solutions”). ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 417 Cat.No. 114687, and thermoreactor is necessary. zinc standard solution Certipur , Cat.No. 119806, concentration 1000 mg/l Zn, can be used after diluting  Result can be expressed as sum of zinc ( Zn). accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 418 Certipur Cat.No. 119806, concentration 1000 mg/l Zn, can also be used after diluting accordingly. To check for sample-dependent effects the use of addition solutions (e.g. in CombiCheck 40) is highly recommended. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 419 To check the measurement system (test reagents, AutoSelector. cell compartment. measurement device, and handling) ready-for-use zinc ® standard solution , Cat.No. 119806, concentration Certipur 1000 mg/l Zn, can be used after diluting accordingly. ® Release 06/2014 - Spectroquant UV/VIS Spectrophotometer Pharo 300...
  • Page 420 Spectroquant ® photometers NOVA 60 and Pharo photometers Suitability of Test Kits for Testing Seawater and Tolerance Limits of Neutral Salts Test kit Cat. No. Seawater Limit of tolerance, salts in % NaCl NaNO Acid Capacity Cell Test 101758 –...
  • Page 421 Spectroquant ® photometers NOVA 60 and Pharo photometers Suitability of Test Kits for Testing Seawater and Tolerance Limits of Neutral Salts Test kit Cat. No. Seawater Limit of tolerance, salts in % NaCl NaNO Iron Test 114761 Iron Test 100796...
  • Page 422 Spectroquant ® photometers NOVA 60 and Pharo photometers Suitability of Test Kits for Testing Seawater and Tolerance Limits of Neutral Salts Test kit Cat. No. Seawater Limit of tolerance, salts in % NaCl NaNO Surfactants (cationic) Cell Test 101764 Surfactants (nonionic) Cell Test...
  • Page 423 Spectroquant ® photometers NOVA 60 and Pharo photometers ® Spectroquant CombiCheck and Standard Solutions Test kit, Evalu- CombiCheck, Confidence interval Diluted and ready-to-use Ready-to-use Cat. No. ation Cat. No. Spec. value max. standard solutions, CRM standard or method for the working Cat.
  • Page 424 Spectroquant ® photometers NOVA 60 and Pharo photometers ® Spectroquant CombiCheck and Standard Solutions Test kit, Evalu- CombiCheck, Confidence interval Diluted and ready-to-use Ready-to-use Cat. No. ation Cat. No. Spec. value max. standard solutions, CRM standard or method for the working Cat.
  • Page 425 Spectroquant ® photometers NOVA 60 and Pharo photometers ® Spectroquant CombiCheck and Standard Solutions Test kit, Evalu- CombiCheck, Confidence interval Diluted and ready-to-use Ready-to-use Cat. No. ation Cat. No. Spec. value max. standard solutions, CRM standard or method for the working Cat.
  • Page 426 Spectroquant ® photometers NOVA 60 and Pharo photometers ® Spectroquant CombiCheck and Standard Solutions Test kit, Evalu- CombiCheck, Confidence interval Diluted and ready-to-use Ready-to-use Cat. No. ation Cat. No. Spec. value max. standard solutions, CRM standard or method for the working Cat.
  • Page 427 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of acid capacity Reagents required: 1.09141.1000 Sodium hy- droxide solution Preparation of a standard solution: 0.1 mol/l A sodium hydroxide solution of 0.1 mol/l (corresponds to 100 mmol/l) is Titripur ®...
  • Page 428 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of calcium Reagents required: 1.02121.0500 Calcium nitrate tetrahydrate Preparation of a standard solution: for analysis Dissolve 2.946 g of calcium nitrate tetrahydrate with distilled water in a EMSURE ®...
  • Page 429 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of free chlorine analogous to Reagents required: DIN EN ISO 7393 1.02404.0100 Potassium iodate, volum. standard Preparation of a KlO stock solution: 1.05043.0250 Potassium iodide Dissolve 1.006 g of KlO...
  • Page 430 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of free chlorine Reagents required: 1.00316.1000 Hydrochloric acid 25 % Preparation of a stock solution: for analysis First prepare a 1:10 dilution using a sodium hypochlorite solution con- EMSURE ®...
  • Page 431 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of chlorine dioxide analogous to Reagents required: DIN EN ISO 7393 1.02404.0100 Potassium iodate, volum. standard Preparation of a KlO stock solution: 1.05043.0250 Potassium iodide Dissolve 1.006 g of KlO...
  • Page 432 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of COD/chloride Reagents required: 1.02400.0080 Potassium hydrogen Preparation of a chloride dilution solution: phthalate GR Dissolve 32.9 g of sodium chloride GR with distilled water in a calibra-...
  • Page 433 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of formaldehyde Reagents required: 1.04003.1000 Formaldehyde solution min. Preparation of a stock solution: 37% GR for In a calibrated or conformity-checked 1000-ml volu metric flask make up analysis 2.50 ml of formaldehyde solution min.
  • Page 434 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of hydrazine Reagents required: 1.04603.0100 Hydrazinium sulfate GR Preparation of a standard solution: for analysis Dissolve 4.07 g of hydrazinium sulfate GR with oxygen-low (boil pre- viously) distilled water in a cali brated or conformity-checked 1000-ml 1.16754.9010 Water for...
  • Page 435 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of iodine analogous to Reagents required: DIN EN ISO 7393 1.02404.0100 Potassium iodate, volum. standard Preparation of a KlO stock solution: 1.05043.0250 Potassium iodide Dissolve 1.006 g of KlO...
  • Page 436 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of monochloramine Reagents required: Chlorine standard solution 100 mg/l Cl Preparation of a standard solution: Preparation see “Standard solution of Place 5.0 ml of chlorine standard solution 100 mg/l Cl and 10.0 ml...
  • Page 437 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of ozone analogous to Reagents required: DIN EN ISO 7393 1.02404.0100 Potassium iodate, volum. standard Preparation of a KlO stock solution: 1.05043.0250 Potassium iodide Dissolve 1.006 g of KlO...
  • Page 438 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of silicate Reagents required: 1.70236.0100 Silicone standard Preparation of a standard solution: solution A silicon standard solution of 1000 mg/l Si is used.
  • Page 439 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of sulfide Reagents required: Sodium sulfide hydrate approx. Preparation of a stock solution: 60 % GR for Dissolve 5.0 g of glass-clear, if necessary washed crystals of sodium...
  • Page 440 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of sulfite Reagents required: 1.06657.0500 Sodium sulfite anhydrous Preparation of a stock solution: for analysis ® Dissolve 1.57 g of sodium sulfite and 0.4 g of Titriplex...
  • Page 441 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of surfactants (anionic) Reagents required: 1.12146.0005 Sodium 1-dode- canesulfonate Preparation of a standard solution: Dissolve 1.00 g of sodium 1-dodecanesulfonate with distilled water in a 1.16754.9010 Water for...
  • Page 442 Spectroquant ® photometers NOVA 60 and Pharo photometers Instructions for the Preparation of Standard Solutions Standard solution of tin Reagents required: 1.70242.0100 Tin standard solution Preparation of a standard solution: ® Certipur A tin standard solution of 1000 mg/l is used.
  • Page 443 Spectroquant UV/VIS Spectrophotometer ® Pharo 300 Operating manual SpectralTransfer...
  • Page 444 Operating manual SpectralTransfer - Backup of measurement data - Backup and recovery of user-defined methods 01/2012...
  • Page 445 SpectralTransfer Contents SpectralTransfer - Contents Overview ........447 Installation .
  • Page 446 Contents SpectralTransfer 01/2012...

  • Page 447: Overview

    SpectralTransfer Overview Overview The SpectralTransfer program is used for the direct data exchange between the photometer and PC. The SpectralTransfer program requires additional software on the PC for the data exchange (see section 2.1). When all programs have been installed, you can: ...

  • Page 448: Installation

    Installation SpectralTransfer Installation PC system requirements ® ®  Windows compatible PC with Pentium or compatible processor (pro- cessor capacity depending on operating system)  Free USB connection ®  Operating system from Windows  Synchronization software: ® ® ® –...

  • Page 449: Establishing The Connection And Starting The Program

    SpectralTransfer Establishing the connection and starting the program Establishing the connection and starting the program Connecting the photometer to the PC A USB cable (type A - type B) is required for the connection. Proceed as follows: Switch on the photometer. Switch on the PC and log in if necessary.

  • Page 450: Starting Spectraltransfer

    Establishing the connection and starting the program SpectralTransfer Starting SpectralTransfer ® In the Windows start menu, click Programs->SpectralTransfer->Spectral- Transfer. The program starts. The SpectralTransfer main window appears. 01/2012...

  • Page 451: Operation

    SpectralTransfer Operation Operation Backing up measurement data Measurement data that should be saved to a PC must be available as *.csv files in the photometer. The following measurement data can be saved:  Exported measurement datasets from the measurement data memory (concentration, absorbance, transmission and multi-wavelengths mea- surements) ...
  • Page 452 Operation SpectralTransfer Example: Dialog box for the backup of measurement datasets (Data Backup - Measured Data) Functions Button Function Opens the directory selection dialog box. Change Here you determine the target directory on your PC. Directory Copies all files from the source directory to the target Copy All -->...

  • Page 453: Backing Up User-Defined Methods

    SpectralTransfer Operation Backing up user-defined methods Here you canbackup and recover alluser-defined methods for the measuring mode, concentration. Thus you can, e.g., transmit the user-defined methods to a different photometer. The backing up of user-defined methods is started in the group field, User defined methods: Functions Button...

  • Page 454: Backing Up The Special/Multi Wave Lengths Methods

    Operation SpectralTransfer Backing up the special/multi wave lengths methods Here you can back up and recover all special/multi wavelengths methods. Thus you can, e.g., transmit the multi wavelengths methods to a different photometer. The backing up of special/multi wave lengths methods is started in the group field, User defined methods: Functions Button...

  • Page 455: What To Do If

    SpectralTransfer What to do if ... What to do if ... Error Cause Remedy – No suitable photometer identi- – Connect the photometer RROR MESSAGE fied Connection failed! – Interrupt and then restore the (the synchronization software USB connection between the did not start automatically) photometer and the PC –...
  • Page 456 Merck KGaA, 64271 Darmstadt, Germany, Tel. +49(0)6151 72-2440 www.analytical-test-kits.com EMD Millipore Corporation, 290 Concord Road, Billerica, MA 01821, USA, Tel. +1-978-715-4321...

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