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Electron Microscopy Sciences EMS 5000 Manual

Tissue slicer
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EMS
5000 Tissue Slicer

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Summary of Contents for Electron Microscopy Sciences EMS 5000

  • Page 1 5000 Tissue Slicer...
  • Page 2 In order that we may properly notify you, we ask you to complete the following information and return to: Quality Assurance Department Electron Microscopy Sciences 1560 Industry Road Hatfield Pa 19440 Serial Number(s) __________ __________ __________ Installation and Functional Checkout Complete per section 2.2 and 2.4 of this manual:...

  • Page 3: Table Of Contents

    TABLE OF CONTENTS EMS 5000 Tissue Slicer 0 Declarations 0.1 Declaration Of Conformity 0.2 Conditions For Use 1 Operational Manual 1.1 Features 1.2 Description 1.3 Operating Environment 1.4 Inventory 1.4.1 Items Described In This Manual 1.4.2 Additional Items Required For Operation 1.4.3 Replacement Items...
  • Page 4 Electron Microscopy Sciences P.O. Box 550 Hatfield Pa 19440 declare that: Equipment: Oscillating Tissue Slicer Model: EMS 5000 Serial Number: ___________ in accordance with the following Directives: 73/23/EEC Low Voltage Directive and its amending directives 89/336/EEC Electromagnetic Compatibility Directive and its amending directives...

  • Page 5: Conditions For Use

    Handling While a high degree of durability has been designed into the EMS 5000, care should be taken not to drop the components. Do not force the tray on the platform, or the pedestal/vise in the tray. Place all cables and leads where they will not be inadvertently pulled or tangled.

  • Page 6: Operational Manual

    1 OPERATIONAL MANUAL 1.1 FEATURES  New slicer head design minimizes vibration that causes tissue distortion.  User defined "travel window" reduces blade travel time between sections.  User selectable multiple slice mode for "hands-free" preparation of many slices.  Consistent live tissue slices of 10 microns achieved.

  • Page 7: Description

    Once the range is set, the START/STOP button is used to toggle the sectioning sequence. The EMS 5000 allows the user to section in two different modes. In manual slice mode, the user can perform all movements of the blade arm using the keypad, or a combination of the keypad and footswitch.

  • Page 8: Inventory

    1.4 INVENTORY 1.4.1 ITEMS DESCRIBED IN THIS MANUAL 1 ea. EMS 5000 Includes: EMS 5000 Footswitch Tray Magnifier Pedestal Specimen Vise Mounting Blocks (2 sizes) Stainless Steel Slicer Blades (25 ea.) 1/16” Hex Driver Manual (Not Shown) A993 OTS5000...

  • Page 9: Additional Items Required For Operation

    1.5 CONCEPTS 1.5.1 DESIGN DESCRIPTION The EMS 5000 slices samples of fresh and fixed tissue, as well as plant and polymer material using an oscillating blade. An embedded microcontroller is used to digitally control functions such as blade position, advance speed, and automatic slice performance. Membrane switch technology in the manual control keypad and polymer packaging protects against corrosion from the laboratory environment.

  • Page 10: Technical Summary

    1.6 TECHNICAL SUMMARY 1.6.1 SPECIFICATIONS EMS 5000: Vertical Travel: 28mm Slice Thickness: adjustable from 1 to 999 microns in 1 micron increments Blade Speed: adjustable from 50 - 5000 cycles per minute Blade Angle Adjustment: adjustable from 15 to 35 degrees...

  • Page 11: Controls/Connectors

    1.6.2 CONTROLS/CONNECTORS Display – EMS 5000 Front Panel Slice Counter / Thickness: Displays a count of the number of slices from a user defined zero reference. When prompted, displays the slice thickness setting in microns. Oscillation: Displays the speed of the blade oscillation in bar graph form. Each line of the graph represents a division between 0 and 5000 cycles per minute.
  • Page 12 SLOW POSITION FORWARD DOWN Controls – EMS 5000 Keypad Blade Position: Forward: Moves the blade arm forward at the set advance speed, and begins blade oscillation. Press once to begin movement, again to stop. Reverse: Moves the blade arm back at the maximum travel speed. Press once to begin movement, again to stop.

  • Page 13: Compatibilities

    Multiple Slice: Start/Stop: Pressing this button enable the travel windows, and causes the EMS 5000 to begin slicing in multiple slice mode. Pressing this button once will cause the blade arm to move forward, and oscillate.

  • Page 14: Illustrative Procedure

    1.7 ILLUSTRATIVE PROCEDURE The EMS 5000 can be operated in two modes. In manual slice mode, the user can perform all movements of the blade arm using the keypad, or a combination of the keypad and footswitch. In multiple slice mode, the EMS 5000 will automatically perform repeatable sequences.
  • Page 15 16. If slicing in Multiple Slice mode, set the forward limit by pressing the FORWARD LIMIT button in the TRAVEL WINDOW section of the keypad. 17. Once the initial slice is performed, press the REVERSE button once to return the blade to a convenient position behind the sample.

  • Page 16: Reference Manual

    2.1.1 PACKAGING The EMS 5000 is enclosed in a two-piece (bottom and back, top) ABS UL94V0 plastic enclosure . The overall dimensions are 27cm x 40cm x 21.5cm (10.5" x 16" x 7.5") The top portion is connected to the interior chassis on the top of the unit, the back panel, and the bottom portion of the case through the baseplate.

  • Page 17: Warranty

    2.1.5 WARRANTY All EMS products are unconditionally guaranteed against defects in workmanship for one year from date of shipment as long as they have been exposed to normal and proper use. Although the one- year warranty may have expired, please contact our Service Department before attempting any repairs or alterations.

  • Page 18: Functional Checkout

    2.3 FUNCTIONAL CHECKOUT 1. Ensure that the unit is installed correctly per section 2.2. 2. Activate power using switch on the back panel. (Indicated by the l side of the rocker switch pressed in.) 3. Ensure that the LEDs in the front of the blade head are illuminated. 4.
  • Page 19 19. Allow the blade arm to travel to its forward mechanical limit. At that point the blade should stop oscillating and return to the reverse limit. Ensure that at the reverse limit, the blade arm stops, steps down, then begins oscillating and moving forward. Ensure that the slice counter increments by 1.

  • Page 20: Operational Information

    2.4 OPERATIONAL INFORMATION The EMS 5000 can be operated in two modes. In manual slice mode, the operator may make a slice and harvest it while the blade arm repositions itself for the next slice. In multiple slice mode, the operator may program the EMS 5000 to automatically slice the specimen block into multiple uniform slices.
  • Page 21 12. Position the pedestal in the tray and set the sample angle if desired. To set the sample angle; use the pivot pins on the side (y axis) and front (x axis) to set the angle, then secure the pedestal by tightening the thumbscrew. Alternately, secure the mounting block in the vise, and position the setup in the tray.
  • Page 22 11. To check or change the slice thickness setting at any time, press either of the THICKNESS buttons once. The display will show the current slice thickness in microns. Pressing either again will change the slice thickness. The new setting will take effect the next time the DOWN button is pressed.

  • Page 23: Scheduled Maintenance

    2.5 SCHEDULED MAINTENANCE The surfaces of the EMS 5000 can be cleaned of any salt buildup etc. by washing with soap and water. DO NOT use phenol or ketones to clean. A yearly performance of the Functional Checkout in section 2.3 should be performed to ensure function and calibration.

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