Diatron Abacus junior 5 User Manual
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Diatron Abacus junior 5 User Manual

Hematology analyzer
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Hematology Analyzer
User's Manual
1.2 release

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Summary of Contents for Diatron Abacus junior 5

  • Page 1 Hematology Analyzer User’s Manual 1.2 release...

  • Page 2: Table Of Contents

    User’s Manual Abacus junior 5 TABLE OF CONTENTS INTRODUCTION............................3 ......................3 NTENDED ................... 3 ENERAL ESCRIPTION 1.2.1 The Instrument ........................3 1.2.2 Patient Testing ........................4 1.2.3 Reagents..........................5 1.2.4 Technical Operation ......................5 1.2.5 Calibration and Quality Control.................... 5 ...................
  • Page 3 © Diatron MI Ltd. 2005 UTILITIES ............................. 37 .......................37 AINTENANCE 7.1.1 Regular Maintenance Jobs....................37 7.1.2 Cleaning ..........................37 7.1.3 Priming..........................38 7.1.4 Draining chambers....................... 38 7.1.5 Empty waste container ......................38 7.1.6 Weekly maintenance ......................39 ......................42 ALIBRATION 7.2.1 Factorial calibration ......................

  • Page 4: Introduction

    User’s Manual Abacus junior 5 1 INTRODUCTION 1.1 Intended Use hematology analyzer is a fully automated cell counter Abacus junior 5 designed for in vitro diagnostic use. The instrument was developed for use in hospitals and small to medium sized labs.

  • Page 5: Patient Testing

    © Diatron MI Ltd. 2005 1.2.2 Patient Testing The analyzer can process 24 blood samples per hour. Samples can have individual sample data, and additional parameters. Full histograms of the blood cell types are also stored along with the above data.

  • Page 6: Reagents

    User’s Manual Abacus junior 5 1.2.3 Reagents Only reagents recommended by DIATRON MI Ltd. should be used with the analyzer, otherwise accuracy cannot be guaranteed. Isotonic saline solution used to dilute whole blood Diluent: specimens and to rinse the fluidic system between measuring procedures.

  • Page 7: Instrument Features

    © Diatron MI Ltd. 2005 1.3 Instrument features Figures 1 and 2 show a front and a rear view of hematology Abacus junior 5 analyzer. Figure 1. Front view Floppy disk drive OK key Numerical keypad Function keys Graphic liquid crystal display...
  • Page 8 User’s Manual Abacus junior 5 Figure 2. Rear view 1. Reagent tubing connections 2. On/Off switch 3. External power supply inlet 12VDC 4. PS2 external keyboard port 5. USB port 6. Serial (RS 232) port 7. Parallel printer port...
  • Page 9 © Diatron MI Ltd. 2005 Figure 3 shows an open built-in printer using roll paper inside. To open the lid, press the button indicated on the picture. Just drop thermal printer paper and close the lid so that the end of the paper gets between the black paper guide and the printer mechanics.

  • Page 10: Parts Of The Analyzer

    User’s Manual Abacus junior 5 1.4 Parts of the Analyzer hematology analyzer can be divided into three main parts: Abacus junior 5 Performs sampling, diluting, mixing, and lysing Fluidic System: functions. Generates regulated vacuum used for moving cells through the aperture during the counting process.

  • Page 11: Control Panels

    © Diatron MI Ltd. 2005 1.4.2 Control Panels START button Pressing and releasing the START button triggers an analysis cycle. Status indicator A three-color LED is located near the START button. Its actual color indicates the status of the analyzer.
  • Page 12 User’s Manual Abacus junior 5 Function keys Below you can find all the possible icons and functions assigned to the so-called soft- keys (function keys) Function key Action triggered Exit from actual menu or action Leave data-entry menu without saving any changes made to it...

  • Page 13: Control Material

    Specification (assay values and allowed tolerances along with expiry date) regarding these materials is always enclosed with the approved control material. 1.6 Accessories This list can also be referred as the “DIATRON Abacus junior 5 - pack” Hematology Analyzer...

  • Page 14: Specifications

    User’s Manual Abacus junior 5 1.7 Specifications 90µl whole blood Sample volume: 80µm (RBC) 100µm (WBC,EOS,BAS) Aperture diameter: 22: WBC, LYM, MON, EOS, BAS, NEU, LYM%, MON%, Measured Parameters: EOS%, BAS%, NEU%, HGB, MCH, MCHC, HCT, RBC, MCV, PLT, MPV, PCT, RDWc, PDWc...

  • Page 15: Installation

    © Diatron MI Ltd. 2005 2 INSTALLATION 2.1 General information This chapter provides instructions for the installation of the Abacus junior 5 hematology analyzer. The procedures described below must be followed correctly to ensure proper operation and service. Please carefully read and follow all instructions in this User’s Manual before attempting to operate...

  • Page 16: Unpacking And Installation

    User’s Manual Abacus junior 5 2.3 Unpacking and installation 1. Carefully remove the hematology analyzer Abacus junior 5 from the shipping carton. Inspect the instrument for any visible signs of damage incurred during shipping. If you find any damage, immediately file a claim with the carrier or your distributor. Check the accessories received against the packing list.
  • Page 17 © Diatron MI Ltd. 2005 7. Reagent Containers Place the reagent containers near the instrument, to an accessible location. Do not place the containers to a higher position than that of , because would a tube come off its Abacus junior 5 connector, the fluids spoil out.

  • Page 18: Turning The Instrument On

    User’s Manual Abacus junior 5 2.3.1 Turning the Instrument ON a. In case you use an external printer (for information, read manual shipped with the printer) connect it and turn it on. b. Turn the instrument on by flipping the power switch (above external power supply connector) to I position.

  • Page 19: Turning The Instrument Off

    © Diatron MI Ltd. 2005 2.3.2 Turning the Instrument OFF The instrument should never be switched off by simply pressing the power button on the rear panel. Doing so may result in erroneous operation during later use. The instrument uses different kinds of solutions, one of which is the so-called diluent. This liquid is an isotonic saline solution containing salt.

  • Page 20: Preparing For Shipment

    User’s Manual Abacus junior 5 2.3.3 Preparing for shipment The second item in Exit (Shutdown) menu should be used when the instrument is to be shipped or left unused for a longer time. The instrument will ask you to utilize the cleaning tube kit and 100ml of distilled water.

  • Page 21: Menu System

    © Diatron MI Ltd. 2005 3 MENU SYSTEM 3.1 General Information This chapter contains information about the structure and usage of the software implemented menu structure. This integrated software controls instrument operations including calculation and evaluation of measured data, displaying results and information screens, storage and recalling of data.
  • Page 22 User’s Manual Abacus junior 5 arrow The arrow under the Printing short cut-key indicates Printing function is enabled. b. You can select the desired item with the  a nd keys and press the OK key to enter or activate the highlighted item.

  • Page 23: Menu Structure

    © Diatron MI Ltd. 2005 3.1.2 Menu Structure Measure local F1 Repeat last sample Measure blank 3 part only Needle Height Setting: Database local F1 Go to specified record Selection Select by date time, and ID Change sort order Select all...

  • Page 24: Operating Principles

    User’s Manual Abacus junior 5 4 OPERATING PRINCIPLES 4.1 Impedance Method The volumetric impedance method (a.k.a. Coulter method) counts and sizes cells by detecting and measuring changes in electrical impedance when a particle in a conductive liquid passes through a small aperture.

  • Page 25: Parameters

    © Diatron MI Ltd. 2005 4.3 Parameters measures and calculates 22 different parameters, listed Abacus junior 5 below. For each parameter, you can find name, abbreviation and measurement unit in the first column. Also, a short description for each parameter in the second column.

  • Page 26: Absolute And Linearity Ranges Of Measured Parameters

    User’s Manual Abacus junior 5 4.4 Absolute and Linearity Ranges of Measured Parameters Within linearity range, the instrument is guaranteed to provide the specified accuracy. Beyond this linearity range, the instrument is able to display results, but may not guarantee accuracy defined in specifications.

  • Page 27: Routine Utilization And Measure

    © Diatron MI Ltd. 2005 5 ROUTINE UTILIZATION and MEASURE 5.1 Measuring Process 5.1.1 Sample handling As some time will usually elapse between collection of samples and counting, it is necessary to preserve the sample with an anti-coagulant to prevent large groups of cells forming into clots or lumps of cell matter that will clog the cell counter.

  • Page 28: Sample Analysis

    User’s Manual Abacus junior 5 The sample rotor will turn into inside of the instrument and the needle draws 90µl of sample from the tube. The aspirating needle is retracted, while its outer surface is automatically rinsed with diluent. This insures a low carry-over between samples.

  • Page 29: Sample Information

    © Diatron MI Ltd. 2005 5.2.3 Sample information The software allows the user to enter information for each sample that has been, or is to be measured. If an external PC keyboard (standard USA layout) is used, it must be connected to the instrument before turning the instrument on.
  • Page 30 User’s Manual Abacus junior 5 In the followings we summarize the warning flags and give an explanation of their possible cause and a few hints to overcome the problem: Uppercase letters refer to WBC or HGB problems: Flag Meaning Recommended user action...
  • Page 31 © Diatron MI Ltd. 2005 Warning flags in lowercase refer to RBC or PLT problems: Flag Meaning Recommended user action PLT blank is high, or no Run cleaning and repeat the blank measurement. PLT blank Diluent or system cleanliness problem.

  • Page 32: Measure

    User’s Manual Abacus junior 5 5.3 Measure Pressing the MEASURE short-cut key, the measurement screen is displayed. The default measurement screen is set to Human profile. Patient data entry screen is accessible by pressing the button. Patient data entry screen allows entering...

  • Page 33: Measure Local Menu

    © Diatron MI Ltd. 2005 5.3.1 Measure local menu From the Measure local menu you can access further sub-menus. 5.3.1.1 Repeat last measurement Choosing item 1 from the Measure local menu the last measurement can be repeated and an R sign will be displayed on the upper left corner of the screen.
  • Page 34 User’s Manual Abacus junior 5 Parameter 1. No flag at parameter 2. * flag at result 3. E (error) flag at result 0-10 g/l 10 - 25 g/l > 25 g/l 0 - 0.5 x10 cells/µl 0.5 - 1.0 x10 cells/µl...

  • Page 35: Database

    © Diatron MI Ltd. 2005 6 DATABASE Patient results are stored in the memory in chronological order, and can be retrieved at any time. Data storage capacity is 1,000 measurements, including complete parameter list, histograms, flags, sample data, and date/time of measurements.
  • Page 36 User’s Manual Abacus junior 5 <DATABASE LOCAL MENU> SELECTION (2) Besides checking samples one-by-one, selection can also be done within Database local menu. SELECT ALL checks all boxes, DESELECT ALL clears all boxes. <DATABASE LOCAL MENU> SELECTION (2) SELECT BY DATE, TIME AND ID...

  • Page 37: Diatron Mi Ltd

    © Diatron MI Ltd. 2005 When you have selected the day or data to ! Message 5104/12210 be saved, and confirmed it with the 35 data record(s) will be saved on 1 disk(s). key, the instrument will prompt you for an Insert an empty floppy disk! empty disk.

  • Page 38: Utilities

    User’s Manual Abacus junior 5 7 UTILITIES 7.1 Maintenance By selecting item (1) of the UTILITIES you can access the MAINTENANCE menu. 7.1.1 Regular Maintenance Jobs Maintenance submenu offers tools including maintenance procedures such as cleaning, priming, draining. MAINTENANCE (1) Select the required submenu.

  • Page 39: Priming

    © Diatron MI Ltd. 2005 7.1.3 Priming During the priming cycle, the fluidic system is rinsed with a large amount of diluent. It differs from the process in a start-up procedure; as in the latter case a simple filling up of the fluidics is performed.

  • Page 40: Weekly Maintenance

    User’s Manual Abacus junior 5 WASTE HANDLING – VERY IMPORTANT Waste contains poisonous substances (because of possible cyanide content) and human origin substances causing biohazard. These substances are representing potential danger to environment. For this reason, safe handling of the waste liquid is very important.
  • Page 41 © Diatron MI Ltd. 2005 Sampler, blood detector Microdilutor Chambers: Reagent grounding Figure 9. Side view Valve block Reagent sensors Dilutor Peristaltic pump Reagent connectors Valve block Figure 10. Rear view...
  • Page 42 User’s Manual Abacus junior 5 7.1.6.1 Peristaltic pump maintenance The pump installed in the instrument is maintenance free. However, would you experience leakage from the pump, or get vacuum error displayed, you may replace the tube used. 1. Open the rear door.

  • Page 43: Calibration

    © Diatron MI Ltd. 2005 7.2 Calibration Calibration is the procedure used to standardize the instrument by applying necessary correction factors. It is recommended to do calibration in the following cases: 1. At analyzer installation, before beginning the analyses. 2. After replacing any component, related to the process of dilution or measurement.

  • Page 44: Factorial Calibration

    User’s Manual Abacus junior 5 7.2.1 Factorial calibration If the CALIBRATION MODE has been previously set to Factorial Calibration, the factors can be set manually in the 0.80 - 1.20 range. UTILITIES CALIBRATION (2) CALIBRATE (1) (factorial) Enter previously calculated factors using numerical keys;...

  • Page 45: View Calibrations

    © Diatron MI Ltd. 2005 After entering the required reference values, perform analyses on the control material. Press to accept results. number calibration analyses performed is shown on the first line. Following calibration, the new factors are Calibration displayed. The previously used factors are 1.02...

  • Page 46: Quality Control

    User’s Manual Abacus junior 5 7.3 Quality Control By analyzing control materials, day-to-day reproducibility can be monitored. Both target values and acceptable ranges for each parameter can be specified for different QC levels herein. provides six different Quality Control levels. You can Abacus junior 5 configure up six individual reference sheets for each control material (e.g.

  • Page 47: Qc Database

    © Diatron MI Ltd. 2005 NOTE: Quality control measurements can only be made after an optimal blank measurement result has been accepted (all parameters were in the 1 range). UTILITIES QUALITY CONTROL (3) QC MEASURE (2) After selecting target values (or targeted level), use the above menu to perform a QC analysis.

  • Page 48: Diagnostics

    User’s Manual Abacus junior 5 UTILITIES QUALITY CONTROL (3) VIEW QC DIAGRAM (4) Means, standard deviations (StDev) and coefficients variation (CVar) calculated based on the QC analyses. The dotted lines delineate acceptable ranges on Levey-Jennings charts. You can look at Quality Control information and results in table format as well. To access this format, select item 3 View table of QC measures of Quality Control.

  • Page 49: Self Test

    © Diatron MI Ltd. 2005 7.4.2 Self test The Self test is a procedure to verify proper operation of essential components of the instrument. The Self test should be performed: • At installation. • After replacing any component. • After extended time out of use.

  • Page 50: Settings

    User’s Manual Abacus junior 5 7.5 Settings Selecting item four (4) of the UTILITIES you can access this menu. The SETTINGS sub-menu allows the user to set the fluid detector operation, printing parameters, dates, user modes and various data. 7.5.1 Printer Settings Make sure to select the appropriate printer mode for correct operation before printing.
  • Page 51 © Diatron MI Ltd. 2005 To set up the instrument for your printer, go to the “Utilities/Printer/Printer Settings” menu. Select from the options using the up and down arrow keys within the text fields, and fill in the numerical fields using the number keys.
  • Page 52 User’s Manual Abacus junior 5 Table format defines how a table-printout will look like. You can select normal or narrow view. A normal table is as wide as the paper, narrow format has parameters grouped such, that Printer cable defines which communication is to be used between instrument and printer.
  • Page 53 © Diatron MI Ltd. 2005 Vertical spacing will determine how much space should be left between two reports printed on the same sheet (One result per page must be OFF to let this option take effect) Limits format defines what the ranges should look like on the printout.
  • Page 54 User’s Manual Abacus junior 5 7.5.1.1 Troubleshooting guide for printing problems Problem Possible reasons/remedies Printer does not respond, • Printer is off. Turn it on. no printout. • Printer is not connected to analyzer. Connect it to the parallel or USB port of analyzer.

  • Page 55: Customize

    © Diatron MI Ltd. 2005 7.5.2 Customize SETTINGS (5) CUSTOMIZE (2) This is a collection of settings influencing instrument operation, customization. 7.5.2.1 General settings SETTINGS (5) CUSTOMIZE (2) GENERAL SETTINGS (1) This is a collection of settings influencing instrument operation, customization.
  • Page 56 User’s Manual Abacus junior 5 7.5.2.3 Laboratory This menu allows entering laboratory information. This will be printed in the header of reports printed by the instrument. SETTINGS (4) CUSTOMIZE (2) LABORATORY (5) A maximum of 40 characters can be entered in each line. User can enter this...
  • Page 57 © Diatron MI Ltd. 2005 With multi-user mode enabled, users with different access levels will have different abilities within the menu system. Some items will not be accessible for them. USER MODES (6) This menu allows configuring instrument for single-user or multi-user mode.
  • Page 58 User’s Manual Abacus junior 5 7.5.2.4.1 Multi-user mode If the analyzer is in multi-user mode, a login screen will be displayed during start-up, asking for a user ID and a password. After the User ID is entered and confirmed with...

  • Page 59: Date And Time

    © Diatron MI Ltd. 2005 7.5.3 Date and Time The date and time of each analysis is stored with the results. This menu allows setting the built-in clock and the format of the date displayed. SETTINGS (5) DATE AND TIME (2) By selecting item 1, you will enter date and time setting mode (next screen).

  • Page 60: Software Update

    User’s Manual Abacus junior 5 7.6 Software Update Instrument software can be updated easily following the steps below: 1. Shut down the instrument, and switch it off. 2. Insert the upgrade floppy disk in the floppy drive. 3. Switch on the instrument and follow the on-screen instructions.

  • Page 61: Printing

    © Diatron MI Ltd. 2005 8 PRINTING This chapter covers information on making reports on measured samples. 8.1 Printouts When required, the following items can be sent to an external printer or to a built-in printer by pressing the function key button.
  • Page 62 User’s Manual Abacus junior 5 Full printout format with histograms: Header Sample and measurement info Report info Histograms Limits, or graphical location of the result within the range Location of of limits warning flags (not displayed now) Figure 16. Built-in printer printout...
  • Page 63 © Diatron MI Ltd. 2005 • Copy the printout to normal paper...

  • Page 64: Fluidic Schematics

    User’s Manual Abacus junior 5 9 Fluidic Schematics BASO Dilu MIXING HEAD V8 DilNeedle Micro V6 DilWash V21Eo V9LyseWbc DILUENT Pressure Meter CLEANER BASO Baso Lyse Puffer BASO BASO LYSE Pump 2-way Valve Liquid Detector 3-way Valve Closed = Off...
  • Page 65 © Diatron MI Ltd. 2005 Revision history: Revision Section Modification C. Magyar 2005.02.15 Revision B. Mendele 2005.02.17 Re-edition, lower DPI pics, etc. A. Gyetvai 2005.04.04...

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