Thermo Hybaid PX2 User Instruction Manual
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Thermo Hybaid PX2 User Instruction Manual

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Thermo Hybaid PX2 User Instruction Manual

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  • Page 1 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...
  • Page 2 THERMO HYBAID Px2 Thermal Cycler USER INSTRUCTION MANUAL Head Office Thermo Hybaid Action Court Ashford Road Ashford Middlesex TW15 1XB Telephone: +44 (0) 1784 425 000 Facsimile: +44 (0) 1784 248 085 Internet: www.thermohybaid.com STOCK CODE: HB-PX2-MAN FS31999 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 3 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 4: Pcr License Registration

    5' nuclease assays, or to any patent claiming a reagent or kit. Applied Biosystems does not guarantee the performance of this instrument. © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 5 Chassis Heated Lid Height Selector Heated Lid Large LCD Screen Key Pad Main Body Stabilising Feet © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 6: Warranty

    No liability is accepted for loss or damage arising from the incorrect use of the Px2 Temperature Cycling system. Thermo Hybaid's liability is limited to the repair or replacement of the unit or refund of the purchase price at Thermo Hybaid's option. Thermo Hybaid is not liable for any consequential damages.
  • Page 7 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 8: Table Of Contents

    CHAPTER 2: SAFETY PRECAUTIONS................7 General Safety Precautions ...............7 Symbols and Conventions ..............8 CHAPTER 3: UNPACKING & INSTALLATION ............9 Unpacking...................9 Packing List..................9 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 9 Editing an Existing Program..............22 Viewing an Existing Program...............24 Copying Programs ................24 Erasing an Existing Program..............25 Advanced Edit Features ..............25 viii © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 10 Invalid Gradient Parameters ..............29 Gradient Calculator Function...............29 CHAPTER 6: RUNNING A PROGRAM ON THE Px2..........31 The Run Option................31 Running a Program on the Px2 – Short Instructions ......31 Run Screens ..................32 Text Screen..................32 Power Failure Screen................33 End of Run Screen................33 Error Screen..................33...
  • Page 11 APPENDIX III: IN SITU BLOCK APPLICATION NOTES ..........63 APPENDIX IV: GLOSSARY OF TERMS.................65 APPENDIX V: PRE-SET PROGRAMS ON THE Px2 ............69 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 12: Introduction

    Px2 THERMAL CYCLER Introduction 1.1 Overview Px2 is the essence of fast, accurate licensed thermal cycling. Px2 features high capacity, high speed and sub-ambient blocks to perform oil-free thermal cycling with excellent dynamic uniformity and precision control of sample temperature.

  • Page 13: The 384 Well Block Module

    (1mm thick). Heating and Cooling The Px2 sub-ambient blocks are built to proven designs, providing an accurate and reliable thermal cycling system. The sub-ambient aluminium block is heated and cooled by the latest in Peltier technology. With proven durability, the block modules excel in performing applications such as RAPDs and Differential Display, which require cycling temperatures close to ambient.

  • Page 14: Programming And Operation

    The Px2 can thus be programmed to perform all types of temperature controlled reactions, from simple one step incubations, to complex multi-step temperature cycling protocols and temperature gradient experiments*.

  • Page 15: Advanced Edit - Gradient (Optional)

    Advanced Edit – Gradient (optional) For rapid optimisation of primer annealing parameters the Px2 gradient feature should be used. A maximum temperature gradient of 15°C can be programmed across the 96 well block. Calibration of your Px2 Every machine is calibrated using miniature thermistor probes located in tubes or attached to microscope slides.

  • Page 16: Input / Output Sockets

    1.4 Input / Output Sockets • The Px2 has a power input socket located on the back of the unit © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 17: Support Services

    RS232, whilst the position closest to the back of the unit corresponds to RS485. 1.5 Support Services The Px2 has been designed for reliability and for ease of maintenance. Thermo Hybaid continues to offer full service and technical support for all its products. Thermo Hybaid Head Office:...

  • Page 18: Safety Precautions

    2. Before use, ensure that the unit has been set to the appropriate mains voltage. 3. The Px2 is a class 1 appliance. To minimise the risk of electric shock, the unit must be connected to a protective earth via the supplied mains cord.

  • Page 19: Symbols And Conventions

    2.2 Symbols & Conventions The Px2 has been designed for safe operation. The following symbols appear on the unit and their meanings should be noted. Indicates the ON position of the main power switch. Indicates the OFF position of the main power switch.

  • Page 20: Unpacking & Installation

    RS232 Lead NB: The In Situ Flat Block Module includes a humidity chamber. If any item is missing or damaged, contact the UK Service Department, or your local Thermo Hybaid office/authorised distributor. Ensure any padding between the plate inside the heated lid and the block is removed before using the instrument.

  • Page 21: Installation

    In Px2 systems, excess heat is removed from the system by a fan. The air intake for the fan is located underneath the unit and air is expelled out of the back of the unit. To ensure correct airflow around the unit, allow at least 8cm clearance between the sides of each module and 15cm of clearance at the back of the unit.

  • Page 22: Thermistor Positioning

    Figure 3.2 Well Numbering Showing Tube Thermistor Positioning in A2 Use in Cold Rooms The Px2 may be used in a cold room (down to 4°C). However, when removed to room temperature the instrument should be allowed to equilibrate for at least two hours before being switched on. If equilibration is not done, condensation may form within the unit and cause a short circuit.
  • Page 23 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 24: Operating Instructions

    Operating Instructions 4.1 Initial Start Up Switch on the Px2 using the power switch at the rear of the unit. When first switched on the unit will complete a start up and self test routine. After 5 seconds a screen will display “Self-check procedure in progress”.

  • Page 25: Operating Keys

    4.3 Operating Keys The Px2 software is designed to be intuitive; the following table gives a summary of the primary functions of each of the operating keys. Table 4.2 Function Keys FUNCTION ENTER KEY Selecting Menu and program choices. MENU KEY Always moves to the Main Menu –...

  • Page 26: Power Failure Resume

    Power Failure Resume If there is a power interruption during a run, the Px2 will switch back on automatically. The unit can be set to resume a program (at the cycle and setpoint where the interruption occurred) or to abandon the program.

  • Page 27: Running A Program

    4.6 Running a Program Design of the Px2 software ensures that running a pre-stored protocol is easy. • At the Main Menu, select RUN by depressing the relevant softkey. • Select the source directory (e.g. F: THERMO) using the directional UP and DOWN softkeys and press ENTER.

  • Page 28: Programming Function

    4.8 Programming Function PCR protocols of varying degrees of complexity can easily be created using Px2. As with all operations of the Px2, programming is accessed from the Main Menu. Access the Program Menu by selecting PROG from the main menu. If at any time you wish to stop programming, press MENU to return to the Main Menu.

  • Page 29: Loading Samples And Heated Lid Operation

    4.9 Loading Samples and Heated Lid Operation The Px2 will produce identical cycling profiles whether 1 or 96 samples are to be analysed. The following guidelines maximise the uniformity and thermal transfer characteristics of the block thus ensuring optimal sample to sample reproducibility: •...

  • Page 30: Programming The Px2

    Programming the Px2 5.1 Introduction The Px2 has been designed to make programming user friendly and easy. The memory will hold up to 99 complete thermal cycling protocols including 10 pre-programmed template programs (Nos. 90-99 detailed in Appendix V). Programs are identified by a number (01-99) and a user defined name (up to 7 characters).

  • Page 31: Editing A Directory Name

    COPY from the program menu. Press ENTER to select. 2. Use the LEFT and RIGHT softkeys to move the cursor to ERASE DIR and press ENTER to select. © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 32: Programs

    4. A final confirmation screen allows you either KEEP or ERASE the directory. 5.3 Programs The programs in the Px2 are divided into ‘stages’ and ‘steps’ when displayed on the screen. In a simple PCR program a stage typically includes three steps where each step refers to the temperatures and times within the protocol.

  • Page 33: Editing An Existing Program

    Note: If there are no free program spaces the Px2 will prompt you to overwrite an existing program. 7. Enter temperature (e.g. 95°C) and time (hr:min:sec), as required. Use the LEFT softkey to move the cursor as desired or ENTER to advance to the next step. The UP softkey can be used to scroll backward to correct a mistake.
  • Page 34 ADVANCED softkey at the relevant step (see section 5.4). It is possible to edit programs whilst the Px2 is running a different protocol. 1. To edit and overwrite existing programs select PROG from the Main Menu using the appropriate softkey.

  • Page 35: Viewing An Existing Program

    ENTER to select. 4. Move the cursor using the UP and DOWN softkeys to select the target directory and press ENTER. © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 36: Erasing An Existing Program

    If Advanced Edit data already exists for any step - <ADV> is indicated on the screen. © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 37: Time Advanced Worked Example

    2. In the EDIT mode (NEW or EXISTING) move Stage 2, Step 3 (relevant extension step) using the navigation keys. 3. Press ADVANCED to access the Advanced Edit screen. © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 38: Temperature Advanced Worked Example

    C - 30s – 1.0°C/cycle x 16 Cycles C - 30s Stage 3: Enter parameters to create: C - 30s © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 39: Changing Ramp Rates

    GRAD section the number will be displayed on the main programming screen. E.g. <G:15>. © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 40: Gradient Programming Worked Example

    If the combination of the Set Annealing Temperature and Gradient results in the block operating outside the temperature limits of 30 to 70 C, the Px2 alters the temperatures to remain within these limits. E.g. SET TEMP = 60 C and GRAD = 15 C.
  • Page 41 The Gradient Calculator may be accessed at any time, whether the block is active or inactive. © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 42 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 43: Running A Program On The Px2

    • Choose whether to run your samples oil-free i.e. heated lid option. Once a program is selected, the program name will be displayed and lid preheating (if selected) will commence. It is possible to edit programs whilst the Px2 is running a different protocol (see Section 5.3).

  • Page 44: Run Screens

    This allows the user to monitor the performance of the Px2 and to determine the time of the end of the program. The second screen is the graphical display which shows the block and tube / sim tube temperatures.

  • Page 45: Power Failure Screen

    • The stage in the run when the power failure occurred. End of Run Screen The Px2 displays a further screen at the end of the run, which provides the following: • The program name and number. • The total run time.

  • Page 46: Extension Lead For Tube Thermistor

    CANNOT be used with Px2 thermal cyclers. Extension Lead for the Tube Thermistor The Px2 is provided with an extension lead for the thermistor. This should only be used for transferring protocols from instruments that cannot use Active Tube Control. See Section 7.3 for further details.

  • Page 47: Simulated Tube Control (Sim Tube)

    The system is optimised for the 0.2ml block with thin walled polypropylene plate. Figure 6.1 Modes of Control used with the Px2 Active Tube Control © Thermo Hybaid. Issue 1.0, January 2002...

  • Page 48: Simulated Slide Control (Sim Slide)

    Using a higher calibration accelerates the approach to target temperature, but results in some degree of overshoot. Conversely, a lower calibration factor slows the approach to target temperature. © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 49: Block Control

    If, after 90 minutes, the CONTINUE key has not been pressed to start a program, a manual start time out screen is displayed and the run is cleared. © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 50: No Heated Lid

    6.6. Hold & Pause Function When entering a program the Px2 will give you the opportunity to enter a ‘Hold’ temperature at the end of each stage. The samples will be held at this set temperature indefinitely. The screen will display HOLD during a run.

  • Page 51: Program Completion

    For most thermal cycling applications a final HOLD at elevated temperature is not necessary. Extended high temperature hold steps can lead to evaporation and condensation problems, particularly with polycarbonate plates. © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 52 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 53: Troubleshooting

    The Px2 block accommodates most types of reaction tubes. The tube thermistor supplied with the Px2 is mounted on an OmniTube, which should be changed periodically, as the fit of the tube will deteriorate with time. Reaction tubes should be distributed evenly in the block. If you are using a reaction tube that has not been recommended, check its fit in the Px2 block before use.

  • Page 54: Programs Using Sim Temperature Control

    Viewing the run screens for the block will indicate the time of the power failure and where applicable the time the run resumed. © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 55: Transfer Of Protocols From A Block Control Machine

    1. Set the Px2 to run a single program at a set temperature for an extended time. (e.g. 37°C for 4 hrs), ensuring that the heated lid is switched off and the instrument is set to block control. This enables the tube thermistor to be used remotely.

  • Page 56: Denaturation

    70-75°C (see data sheet from manufacturer). The time required depends on the length of product being synthesised. © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 57 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 58: Maintenance

    Thermo Hybaid office or authorised dealer to check that the proposed method will not damage the equipment. All surfaces of the Px2 system and heated lid should be cleaned regularly with a soft cloth, hot water and a mild detergent.

  • Page 59: Protection To The User

    8.4 Protection of the Instrument Fuses The Px2 mains power inlet is fitted with two T6.3A fuses (20mm x 5mm). If necessary these may be replaced by a qualified person. 8.5 Tube Thermistor Care The tube thermistor assembly ensures that the programmed temperature profile is accurately and reproducibly achieved within all sample tubes.
  • Page 60 To maintain accuracy and longevity of the tube thermistor Thermo Hybaid recommends the following: 1. Do change the tube thermistor annually. It may be necessary to change the thermistor more often under heavy use (e.g.
  • Page 61 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 62: Technical Specifications & Ordering Information

    Ingress Protection Rating 20 Standard Accessories Tube Thermistor 0.5ml Tube Thermistor 0.2ml (HBPXTTM05) (HBPXTTM02) Interchangeable Block HBPXB05 HBPXB02 Modules Px2 Chassis HBPX2 HBPX2 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 63 Heated Lid Temperature Range 95°C - 120°C 95°C – 120°C Display Resolution 0.1°C 0.1°C Ingress Protection Rating Standard Accessories Humidity Chamber Interchangeable Block HBPXBFB HBPXB384 Modules © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 64 Description Px2 with 0.5ml Gradient Block Px2 with 0.2ml Gradient Block Catalogue Number HBPX2 HBPX2 Block Capacity 48 x 0.5ml tubes 96 x 0.2ml tubes 96 x 0.3ml tubes 1 OmniUltra Plate 1 x 96 well plate Temperature Control Tube control...
  • Page 65 File protection Output RS232 and RS485 Alphanumeric programming Gradient software Power 550W Dimensions (WxDxH) 240mm x 390mm x 280mm Weight 8.6kg © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 66 Power requirements: 550W at 115/230V a.c. + 10% and 50/60Hz. Satisfies the requirements of BS EN 61010-1:1993. When the Px2 thermal cycler is removed from a cold room, it should be left to equilibrate for 2-3 hours to avoid condensation.
  • Page 67 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 68: Appendix I: Recommended Consumables

    OmniFast 96 Polypropylene 96 well plate HB-TC-8002N OmniFast 384 Polypropylene 384 well plate HB-TC-3840N OmniTube 96 Single piece polypropylene plate HB-TC-6002N © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 69 Polycarbonate can be affected by contact with formamide, DMSO and other organic solvents; these should therefore be avoided when using the polycarbonate OmniUltra Plate © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 70 OptiTape or TDX Tape can be removed from the plate with ease. OptiTape Supplied on a roll (with optional dispenser) and designed for use with Thermo Hybaid’s OmniUltra Plate, although will give an exceptional seal with other brands of polycarbonate plate.
  • Page 71 OptiTape is designed for single use. Once heated and compressed, the backing conforms to the plate and cannot be easily repositioned. Reuse is therefore not advised. © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 72 The plugs are shaped to expand sideways in order to seal all 96 wells of a 96 well plate, when sufficient pressure is exerted to the upper surface of the mat by the Px2 heated lid. Figure 10.1: Showing how the OmniSeal TD Mat seals the wells of a 96 well plate.
  • Page 73 Typically volume loss of 3µl per well in a 30-cycle reaction can be expected. The Thermo Hybaid OmniUltra helps to address this problem to some extent. In our tests reaction volumes of 10µl gave strong results. 5µl reaction volumes gave visible but faint results thus 10µl is the minimum recommended sample volume for this plate.

  • Page 74: Appendix Iii: In Situ Block Application Notes

    In Situ Block Application Notes Overview The Px2 In Situ module with flat block has been designed for the precise control of temperature on up to four standard microscope slides (dimensions 76mm x 26mm x 1mm). Flat blocks are supplied with a Humidity Chamber, which can be used to reduce sample drying.
  • Page 75 3°C. Programming Programming and operation of the In Situ module are identical to the standard Px2 tube modules (see Chapter 5) with the exceptions previously noted and also no heated lid operation is supported. For simple fixed temperature incubations of the slides, manual control can be used (see section 4.7). For more complex multi-step incubations or for temperature cycling reactions a protocol can be designed and run using Simulated Slide Control (see Chapter 6).

  • Page 76: Appendix Iv: Glossary Of Terms

    In Situ Module The Px2 Flat Block, for performing In Situ reactions on up to four microscope slides. Loading Factor To compensate for differing numbers of samples, enter the number of tubes for each cycling run.
  • Page 77 Reversing the current reverses the heat flow. Power Failure The Px2 has the facility to restart after a mains power supply failure. There are two set up options for restarting after a power failure: 1.
  • Page 78 Sub-ambient Block The aluminium block used on sub-ambient Px2 systems. This uses Peltier devices for heating and cooling. Operating range: 4°C to 99°C.
  • Page 79 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 80 APPENDIX V Px2 THERMAL CYCLER Pre-Set Programs on the Px2 Programs coded into the F:HYBAID Directory of the Px2 memory can be used directly or as building blocks for other protocols using the COPY function. PROGRAM F:90 3T_PCR Stage 1...
  • Page 81 Stage 4 Step 1 94°C 0:00:30 Step 2 50°C 0:00:30 Step 3 72°C 0:00:30 Stage 5 Step 1 72°C 0:05:00 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 82 0:00:30 Ramp 1.0°C/Sec Step2 55°C 0:00:30 Ramp 1.0°C/Sec Step 3 72°C 0:00:30 Ramp 1.0°C/Sec Stage 3 Step 1 72°C 0:05:00 © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 83 Stage 1 Step 1 37°C 4:00:00 PROGRAM F:99 CUT/KIL Stage 1 Step 1 37°C 4:00:00 Step 2 95°C 0:15:00 Hold 4°C © Thermo Hybaid. Issue 1.0, January 2002 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 84 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...

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