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Table of Contents
Summary of Contents for KSV 5000
- Page 1 Operation Manual LB device KSV 5000 Revision 1.1...
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Page 2: Table Of Contents
Table Of Contents INTRODUCTION------------------------------------------------------------------------- 1 PHYSICAL DESCRIPTION ----------------------------------------------------------- 2 2.1. Overview ------------------------------------------------------------------------------2 2.2. System 1 -------------------------------------------------------------------------------3 2.3. System 2 -------------------------------------------------------------------------------4 2.4. System 3 -------------------------------------------------------------------------------5 2.5. Mechanics -----------------------------------------------------------------------------6 2.6. Additional Devices ---------------------------------------------------------------- 10 2.7. Special Troughs-------------------------------------------------------------------- 11 PRELIMINARIES -----------------------------------------------------------------------15 3.1. Connections------------------------------------------------------------------------- 15 3.2. -
Page 3: Introduction
There are four series of LB systems available today, the KSV 5000, 2000, Minitrough and the Minimicro in descending order of size. The differences between the systems are mechanical, the same software and interface unit operates all of the LB devices. -
Page 4: Physical Description
2. Physical Description 2.1. Overview The general physical layout of the KSV 5000 Langmuir-Blodgett (LB) system consists of the trough with the subphase and the surfactant, barriers which can adjust the surface pressure by reducing the area available to the... -
Page 5: System
2.2. System 1 The appearance of the instrument depends on the system in use. System 1 is the most basic setup, consisting of a balance and a trough with a barrier drive system. Image 2.1: KSV 5000 System 1. Balance Trough Barriers LayerBuilder System 1 is designed for the study of floating monolayers. -
Page 6: System
2.3. System 2 The System 2 additions over System 1 are the dipper, a second stand for the dipper and a trough with a well. Image 2.2: KSV 5000 System 2. Balance Trough Barriers LayerBuilder Dipper Well System 2 is designed for the study of floating monolayers and for the deposition of multilayer films of one substance on a solid substrate. -
Page 7: System
In addition to system 1 the trough is fitted with a bigger, half circle well and a two-armed dipper is positioned above the well. Two pairs of barriers and two balances operate different sides of the trough. Image 2.3: KSV 5000 System 3. Balances 4) LayerBuilder... -
Page 8: Mechanics
2.5. Mechanics A) Computer The computer controls all functions of the instrument. Because each device includes its own microprocessor, the computer sends only high level commands to them (for instance "Move with certain speed" or "Go to certain position" and so on). All devices are listening to every message, but they become active only when they recognise their own address. - Page 9 Image 2.4: Block diagram of the barrier driving system C) Surface Balance The surface pressure is measured by using the Wilhelmy plate method (round rod optional). The Wilhelmy plate is a carefully sandblasted platinum plate or a clean paper plate, which is put partly under the surface of the subphase. Normal practice is to position the plate so that one third of it is under the subphase.
- Page 10 Image 2.5: Block diagram of the surface balance. D) Deposition system The deposition system is DC motor controlled to ensure vibration-free operation. The speed range of the dipping arm is 0.1...85 mm/min and maximum stroke is about 145 mm. The DC motor is servo controlled to keep the speed constant.
- Page 11 Image 2.6: Block diagram of the deposition system. E) Trough and Barriers The troughs are made of a solid piece of PTFE. The trough is glued onto an aluminium base plate, in which there are water channels for the thermostation of the subphase.
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Page 12: Additional Devices
Connect the water bath to the ports along one end of the trough. KSV Instruments recommends the Julabo water bath also available from KSV, though others can be used as well. -
Page 13: Special Troughs
F) Surface Potential Probe SPOT A surface potential probe (KSV SPOT) is a tool for measuring the surface potential of an interface or a solid. KSV SPOT uses the vibrating plate capacitor method. - Page 14 B) Conductivity Trough The conductivity trough is used to measure the conductivity of the monolayer. Four electrodes are placed near the center of the trough, two on each side of a central rise. The central rise divides the trough into two compartments with a narrow band of glass connecting the subphases of the different compartments.
- Page 15 interacts with the monolayer the amount of monolayer material decreases and surface pressure decreases. The barriers approach the centre to keep the surface pressure (and thus mean molecular area) constant, and the area removed by them can be used to calculate the activity of the enzyme. A stirrer might be necessary to properly observe the activity of the enzyme.
- Page 16 G) Custom Troughs Many situations arise where a custom designed trough is necessary to accomplish a particular task. KSV Instruments supplies many custom designed troughs, be they extensions of current designs or completely new concepts. Ask us for more information about a trough that suits your needs!
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Page 17: Preliminaries
3. Preliminaries 3.1. Connections Check that all devices are attached to the LayerBuilder interface unit. Check that the power cable is connected to the interface unit and that it is turned on. Turn on the computer and start the sgserver for LB systems. It can be located from the Start menu under Programs >... -
Page 18: Cleaning
The importance of cleanliness cannot be overstated! The troughs used by KSV can be removed from the frame and carried to a sink for ease of cleaning. A recommended cleaning procedure is to first brush with a soft brush covered in ethanol or another organic solvent (mechanical and chemical cleaning) and then rinse with ion exchanged water. - Page 19 Image 3.4: Replacing the trough so that the screws slot into the respective notches. Clean the barriers. The appropriate cleaning method is the same as with the trough. When one barrier is cleaned place it on top of the trough before cleaning the other barrier.
- Page 20 Image 3.5: Sucking contaminants from the barriers. Clean the Wilhelmy plate. A thorough cleaning entails heating the platinum Wilhelmy plate over a flame (such as a Bunsen burner). Heat the plate over a blue flame hot enough to make the plate glow red in just a few seconds, a flame that is too cool will leave residues on the plate surface.
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Page 21: Dipper Substrate Attachment
3.3. Dipper Substrate Attachment If about to perform a Dipping Experiment with a hydrophilic substrate then clean the sample as appropriate. In the Manual Control Unit open the Dipper1 tab and press Up and wait until the dipper arm moves well above the surface. -
Page 22: Experimental Setup
Image 3.8: Place the sample onto the water with care. Wait 10-15min for the solvent to evaporate. Use this time to fill in the Experimental Setup for this experiment. Press on the appropriate icon to start a new experiment, this will first open the Experimental Setup. Double- check that the dimensions of the trough are correct as this plays an important part in the analysis of the obtained data. -
Page 23: Technical Specifications
4. Technical Specifications 4.1. Deposition System Control and operation Software controlled unsupervised and automatic film deposition. User defined deposition parameters. Deposition speed 0.1 to 85 mm/min Speed adjust Increment 0.1 mm Optional speed range 0.2 to 170 mm/min Deposition cycles unlimited consecutive depositions... -
Page 24: Trough
barriers. Hydrophobic (Teflon) barriers by request or optional. Barrier speed 0.01 mm to 400 mm/min (max. relative speed 800 mm/min) Barrier motor High precision micro step driven stepper motor. Accuracy Greater than 99% 4.4. Trough Dimensions System 1: surface area 870 cm (L580 W150 subphase volume 780 ml. -
Page 25: Contact Information
5. Contact Information If any problems arise please feel free to contact a local distributor or KSV Instruments directly. KSV Instruments can be contacted from this address: KSV Instruments Ltd. Höyläämötie 7 FIN-00380 Helsinki Finland Tel. +358-(0)9-5497 3300 Fax +358-(0)9-5497 3333 E-mail info@ksvltd.fi for sales and...
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