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Summary of Contents for Gatan ORIUS SC 200D
- Page 1 ORIUS™ SC 200D CCD Camera User’s Guide Gatan, Inc. 5794 W. Las Positas Blvd. Pleasanton, CA 94588 (925) 463-0200 FAX (925) 463-0204 September 2008 833.MANUAL Revision 3...
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Page 2: Getting Started 2
Preface About this Guide This ORIUS™ User’s Guide is written to provide a brief description of the camera’s operating principles, instructions on how to install the camera, and an introduction to imaging in DigitalMicrograph. For additional discussion of image processing, refer to the DigitalMicrograph User’s Guide. Preview of this Guide The ORIUS™... - Page 3 Disclaimer Gatan, Inc., makes no express or implied representations or warranties with respect to the contents or use of this manual, and specifically disclaims any implied warranties of merchantability or fitness for a particular purpose. Gatan, Inc., further reserves the right to revise this manual and to make changes to its contents at any time, without obligation to notify any person or entity of such revisions or changes.
- Page 4 Support Contacting Gatan Technical Support Gatan, Inc., provides free technical support via voice, Fax, and electronic mail. To reach Gatan technical support, call or Fax the facility nearest you or contact by electronic mail: • Gatan, USA (West Coast) Tel: (925) 463 0200 Fax: (925) 463 0204 •...
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Page 5: Table Of Contents
Table of Contents Preface i Support iii Table of Contents v List of Figures vii ORIUS™ SC 200D CCD CAMERA USER’S GUIDE ......................1 WARNING ................................VII QUICK REFERENCE .............................. 1-1 GETTING STARTED ............................... 2-1 ... - Page 6 ..........................4-3 FTEN SKED UESTIONS APPENDIX 1 ................................... I HARDWARE DESCRIPTION .............................. I .............................. I YSTEM EQUIREMENTS ................................II ESCRIPTION A3 I ................................III NSTALLATION A3.4 Electrical connections ............................VI ...
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Page 7: List Of Figures
List of Figures FIGURE 2-1 DIGITALMICROGRAPH WORKPLACE FIGURE 2-2 (A) CAMERA MENU (B) CAMERA CONFIGURATION FIGURE 2-3 CAMERA MENU FIGURE 2-4 GLOBAL MICROSCOPE INFO WINDOW FIGURE 2-5 MICROSCOPE MENU ... -
Page 8: Warning
0. WARNING As with any CCD camera or the viewing screen in the TEM it is possible to permanently burn the scintillator material with the electron beam. The new SC200D has been designed specifically to handle the intense beam conditions in Diffraction mode. However, precaution should still be made so as not to cause damage in extreme conditions that require very long exposure times or time periods needed for in-situ recording. - Page 9 • Any condition that places a very bright spot in a single place on the scintillator and shows streaking in the background of the image. ORIUS ™ 833 CCD Camera User’s Guide Series Rev 3 viii...
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Page 10: Quick Reference
1 Quick Reference The steps necessary for operating an ORIUS™ CCD camera are summarized here. • Make sure that the viewing/camera chamber is at the proper vacuum for TEM operation. • Make sure the water and the pressure lines are correctly connected to the camera and proper water pressure is maintained. - Page 11 • Click on Start Acquire button in the Camera Acquire palette (Figure 3- 4). (exposure mode set to User). • Click on Print Preview button on the Toolbar (Figure 3-4) to view image in print preview mode. • Click on Save button (or Save Numbered) icon on the Toolbar (Figure 3-4) to save image in the designated folder.
- Page 12 You can enhance your image by performing Fourier filtering, sharpening, or other types of image processing. 5. Present your data. The most important result of the entire process is the presentation of your data. You can arrange multiple images on a page and annotate your images and page through the use of text, lines, boxes, ovals, or clip-art imported from other applications.
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Page 13: Getting Started
2 Getting Started The following steps will enable the first-time user to switch on the digital camera, launch DigitalMicrograph and prepare for the acquisition of an image. • Make sure that the viewing chamber is at the proper vacuum. • Make sure the water and the pressure lines are correctly connected to the camera and proper water pressure is maintained. -
Page 14: Camera Configuration
Results window 2.2 Camera configuration If the camera is already configured by a Gatan service engineer, this section can be ignored. Before starting acquiring images, it is necessary to configure the camera. This process is shown in Figure 2-2. ORIUS ™ 833 User’s Guide Series Rev 3... -
Page 15: Dark Correction
Figure 2-2 (a) Camera Menu (b) Camera configuration • Select Configure Camera in the Camera menu (Figure (2-2a). • In the Camera configuration dialog box (Figure 2-2b), you can change the name of the camera by typing in the Name field. •... -
Page 16: Gain Normalization
2.4 Gain Normalization It is important to prepare a new gain reference image after the CCD has reached the equilibrium temperature (normally this takes less than 5 minutes). The gain reference image should be checked regularly. To prepare gain reference image, remove specimen from the field of view, evenly spread the illumination across the CCD sensor. -
Page 17: Magnification Calibration
2.5 Magnification Calibration The displayed nominal magnification on TEM is for photographic film and has an accuracy of 5-10%. The CCD camera is located on a different plane (height wise) respect to the film camera. As a consequence, the magnification must be calibrated. The calibration is done using standard calibration samples. At “Low”... -
Page 18: Low Magnification
Figure 2-5 Microscope menu In the “Magnification” section in the “Global Microscope Info” window (Figure 2-4) choose: • Select the checkbox ‘Ask User” next to Magnification • Pull down the menu and select “Always” • Click OK 2.5.1 Low magnification •... -
Page 19: Figure 2-7 Instructions Window
Figure 2-7 Instructions window A red line will appear on the image. • Position it on a feature of known size. • Press OK on the “Calibrate image” window. • Enter the correct distance for the selected feature (for example 10 line pairs of cross grating sample where the distance = 10 x 0.463μm) in the “Calibration”... -
Page 20: High Magnification
Figure 2-9 Choose YES to complete the calibration The calibration can be checked by viewing the calibration table. It should contain pairs of value: the nominal microscope magnification and the calibrated value. To view the magnification table: • Select “Calibrations” from the “Microscope” menu (Figure 2-5). Figure 2-10 Magnification table The first number entry represents the nominal TEM magnification and the second entry is the actual... -
Page 21: Figure 2-11 High-Resolution Image Of A Crystalline Sample
Figure 2-11 High-resolution image of a crystalline sample • Choose “Calibrate image from Diffractogram…” under the “Microscope” menu (Figure 2-5). A diffractogram will be calculated after placing a FFT ROI on the image (Figure 2-12). Figure 2-12 Diffractogram • Follow the on screen instructions (Figure 2-13) •... -
Page 22: Figure 2-13 High Magnification Calibration Instructions
• Press OK to specify the reciprocal unit and the d-spacing (in the corresponding real units) in the next window that appears (Figure 2- 14). Figure 2-13 High magnification calibration instructions Figure 2-14 Calibration window ORIUS ™ 833 User’s Guide Series Rev 3 2-10... -
Page 23: Image Acquisition
3 Image Acquisition This chapter contains some instructions on how to acquire an image and a description of the most frequently used floating windows. Now you are ready to: • Insert the sample • Find a region of interest • Start using the CCD camera to view your specimen. - Page 24 corner and its location can be easily changed by putting the pointer inside the box and dragging it. The Auto Survey updates the image display (brightness and contrast) in real time. Auto Exposure is enabled and disabled by clicking on the Auto Exposure selection box. Generally one does not use Auto Exposure when using Camera View mode of operation.
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Page 25: Figure 3-1 Image Window
Figure 3-1 Image window Figure 3-2 Focus Loupe The acquisition can be stopped by: • Pressing Stop View, or • Pressing the Spacebar. ORIUS ™ 833 User’s Guide Series Rev 3... -
Page 26: Image Notes
3.2 Image Notes The user can type in notes about the image in the Info area of the floating window called Image Info and also the ImageDisplay Info window as shown in Figure 3-3. To access this window, right click on the image and select ImageDisplay from the menu. -
Page 27: Image Acquisition
Figure 3-3b Global Info Tool icon Custom fiields 3.4 Image Acquisition To record the final high resolution image, click on the Start Acquire button in the Camera Acquire palette shown in Figure 3-4. This mode is preset to acquire a full resolution CCD image. “Streaking correction” can also be selected in the Setup, Advanced section for use during acquisition of diffraction patterns. -
Page 28: Save Numbered And File Storage Path
Quick Tip: Clicking on the DigitalMicrograph Help icon will allow you to learn more about the other methods of Saving. Numerous image formats are supported and a unique feature of batch conversion from Gatan .dm3 format to other formats is available. -
Page 29: Page Mode (Report) Options
Figure 3-5 Save Numbered Info pane 3.5.3 Page Mode (Report) Options In the Global Info dialog, under Databar you can choose Page mode and Image mode in the left pane. Select Page Mode to view the checkbox items that can be selected in the right pane. Any items selected will be available in the Page Mode Report format (Figure 3-6). -
Page 30: Report (Print Preview)
Figure 3-6 Data Bar – Page Mode Report parameters Custom Data button allows the user to add addition custom fields that were previous created to be also included in your report. This feature is useful to add the name of your institution, ID#’s, or the name of your TEM lab (Figure 3-7). - Page 31 Figure 3-10 (a) Report view (b) Image view Users can customize the layout and selection of parameters on the page (for example, using pointer to move the text around) and then save the setting as a report template. This is done by navigating via Edit, Databar, Save as Global Default in the Menu bar at the top of the main screen (Figure 3-10 c).
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Page 32: Camera Setup For View And Acquire
3.5.5 Camera Setup for View and Acquire In each of the Camera View and Camera Acquire dialog windows (Fig. 3-11) there is a “Setup” button that gives the user access to detail configurations for each of the operation modes (Fig. 3.12a-b). Usually the user does not have to make any changes after the camera has been installed and setup. -
Page 33: Care And Maintenance
4 Care and Maintenance This chapter contains some general tips on the care and maintenance of your ORIUS™ camera. It also contains a list of questions that are often asked by users. 4.1 General Precautions The following are some general precautions users should adhere to in the care and maintenance of their camera. 4.1.1 Peltier cooler maintenance •... -
Page 34: Scintillator Maintenance And Precautions
• Turn off the Peltier cooler if the microscope viewing chamber is to remain at air pressure for a long period (more than 30 minutes to an hour). • Turn the Peltier cooler off if the room the TEM has a dew point above 10C when the TEM is vented. -
Page 35: Most Often Asked Questions
Examine the surface of the scintillator. If the surface of the scintillator becomes dirty or scratched, ship the camera to Gatan for service. Do not attempt to clean the scintillator yourself as damage can result. 4.2 Most Often Asked Questions The following are some of the questions most frequently asked by camera users. - Page 36 8. How long is the lifetime of the CCD? None of Gatan’s CCDs have failed due to old age so it is hard to answer. So far, there are no known mechanisms for aging.
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Page 37: Hardware Description
Available microscope bottom port or 35mm port (If model is available) Water and air connections Gatan Microscopy Suite (GMS) software 1.7.1 or later FireWire 800 also called 1394b capability (will run from 1394a but it is not recommend) ORIUS™ User’s Guide Series Rev 3... -
Page 38: A2 Description
A2 Description An ORIUS™ SC 200D CCD camera acquires an electron microscope image into a computer in four stages. First a scintillator converts the electron image into a high-resolution light image. Then a lens system captures the light from each point in the image and channels it to the CCD. The CCD then converts the transferred light image into a second electron image. -
Page 39: A3 Installation
Controller’s back panel A3 Installation If you have not been trained by Gatan then you must not attempt to install the camera. Installation by an untrained person will invalidate the warrantee and may cause damage to the equipment and injury to the installer. - Page 40 Figure A4 Camera Pneumatic Interface Details ORIUS ™ 833 User’s Guide Series Rev 3...
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Page 41: Figure A5
Figure A5 Water lines ORIUS ™ 833 User’s Guide Series Rev 3... -
Page 42: A3.4 Electrical Connections
A3.4 Electrical connections The diagram in Figure A6 shows the electrical connections in the system. Figure A6 Electrical diagram. ORIUS ™ 833 User’s Guide Series Rev 3... -
Page 43: Environmental Specifications And Safety Requirements
Appendix 2 Environmental specifications and safety requirements ORIUS ™ 833 User’s Guide Series Rev 3... -
Page 44: Tem Requirements
TEM Requirements The ORIUS™ camera should only be used with the TEM’s listed on Gatan’s compatibility chart or specifically approved in writing by Gatan Engineering. The camera should only be used in a manor described in this manual, all other uses could cause system failures and/or dangerous conditions. -
Page 45: Camera Specifications
Appendix 3 Camera specifications ORIUS™ User’s Guide Series Rev 3... - Page 46 Computer interface 1394 IEEE Auto exposure YES (exposure estimation takes less than 5 sec) PTB standard X-ray shielding CE (class B) and UL EMC compliance Installation by Gatan Required Computer platform Windows XP ORIUS ™ 833 User’s Guide Series Rev 3...
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Page 47: Ce Certification
Appendix 4 CE certification ORIUS ™ 833 User’s Guide Series Rev 3... - Page 48 ORIUS ™ 833 User’s Guide Series Rev 3...
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Page 49: Index
Index A G Auto Exposure, 1-2, 3-2 gain reference, 1-1, 2-4, 4-4, 4-5 Auto Survey, 1-2, 3-2 ghost image, 4-5 Global Info, 1-1, 3-4 Global Microscope Info, 2-5, 2-6 B binning, 4-3 H hardware, I C Calibrate image, 2-7, 2-10 I ... - Page 50 Report Options, 3-7, 3-8 Start View, 1-2, 3-2 resolution, 2-10, 3-1, 3-2, 3-5, II retraction, II T TEM, 1-1, 2-5, 2-9, 3-4, 3-8, 4-1, 4-2, 4-3, II S saturation, 4-2 V Saving, 1-2, 2-1, 3-4, 3-5 scintillator, 4-2, 4-3, II vacuum, 1-1, 2-1, 4-2, 4-3, 4-4 Search mode, 1-2, 3-1, 3-2 view mode, 3-1...
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