tecan INFINITE M1000 PRO Instructions For Use Manual
  1. Manuals
  2. Brands
  3. tecan Manuals
  4. Card Reader
  5. INFINITE M1000 PRO
  6. Instructions for use manual

tecan INFINITE M1000 PRO Instructions For Use Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

TECAN
Instructions for Use for
INFINITE M1000 PRO
Document Part No. 30064852
2011-09
Document Version No. 1.0

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the INFINITE M1000 PRO and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for tecan INFINITE M1000 PRO

Summary of Contents for tecan INFINITE M1000 PRO

  • Page 1 TECAN Instructions for Use for INFINITE M1000 PRO Document Part No. 30064852 2011-09 Document Version No. 1.0...
  • Page 2 CAREFULLY READ AND FOLLOW THE INSTRUCTIONS PROVIDED IN THIS DOCUMENT BEFORE OPERATING THE INSTRUMENT. Notice Every effort has been made to avoid errors in text and diagrams; however, Tecan Austria GmbH assumes no responsibility for any errors, which may appear in this publication.
  • Page 3 Indicates a possibility of instrument damage or data loss if instructions are not followed. WARNING INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY, LOSS OF LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS ARE NOT FOLLOWED. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 4 NEGATIVE ENVIRONMENTAL IMPACTS ASSOCIATED WITH THE TREATMENT OF WASTE. DO NOT TREAT ELECTRICAL AND ELECTRONIC EQUIPMENT AS UNSORTED MUNICIPAL WASTE. COLLECT WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT SEPARATELY. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 5 Symbols Manufactured by Date of manufacture USB label Conformité Européenne Consult Instructions for Use Directive 2002/96/EC on waste electrical and electronic equipment (WEEE) symbol Laser Biohazardous 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 6: Table Of Contents

    Fluorescence Polarization Measurement Parameters ... 40     Absorbance System ............41     4.3.1   Absorbance Optics ..............42   4.3.2   Absorbance Detection ............43   Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 7 Fluorescence Intensity and Time Resolved Fluorescence   (TRF) ..................89   6.3.1 Definition of the Detection Limit: ..........89     6.3.2   Fluorescein (Fluorescence Intensity) Top ......89   2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 8 Disposal of the Instrument ........... 128     Error Messages and Troubleshooting ........129     Error Messages Introduction ..........129     Index ....................133   Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 9: Safety

    Users MUST ensure that the plate definition file selected corresponds to the actual plate being used. The safety gaps cannot be calculated by the INFINITE M1000 PRO if the plate used does not match the .pdfx selected. Users should also take care that no potential fluorescent or luminescent...
  • Page 10 Safety data sheets of the reagent manufacturers WARNING Depending on the applications, parts of the INFINITE M1000 PRO may come in contact with biohazardous/infectious material. Make sure that only qualified personnel operate the instrument. In case of service or when relocating or disposing of the instrument, always disinfect the instrument according to the directions given in these Instructions for Use.

  • Page 11: General Description

    Amplified Luminescent Proximity Homogeneous Assays (AlphaScreen and AlphaLISA). The INFINITE M1000 PRO is also robotic compatible and offers a built-in stacker option as well as an external injector module (see picture below). Figure 1: INFINITE M1000 PRO with injector box.

  • Page 12: Multifunctionality

    Switching between measurement techniques or plate formats is fully automated: NO manual adjustments are necessary for the INFINITE M1000 PRO. Injectors are available for microplates from 6 to 384 wells. Tecan also provides a cuvette adapter for four standard cuvettes (e.g.
  • Page 13 (for further details, see the Instructions for Use for the i-control software). Figure 2: Onboard control buttons of the INFINITE M1000 PRO. The ‘Quick-Start- Script’ button and the Retract/Eject button are located in the front right corner of the top cover.

  • Page 14: System Requirements

    .NET Framework. Windows If this version is not present, the install/upgrade program Installer will install it. 2002 2003 Microsoft Excel 2007 2010 (32-bit) – Starter edition NOT supported! Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 15: Measurement Techniques

    The measurement position is not identical to the injector position. The movement between measurement position and inject position takes ≤ 500 ms. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 16 T. Stâhlberg, et.al. - American Laboratory, December 1993 page 15) some of them having fluorescence lifetimes in excess of 100 microseconds. The INFINITE M1000 PRO uses a flash lamp light source with flash duration much shorter than the fluorescence lifetime of these species. This offers the...

  • Page 17: Absorbance

    ® HTRF reagents and technology, and for which Cisbio Bioassays has granted a license to Tecan. The method is covered by the US patent 5,527,684 and its foreign equivalents. C) Fluorescence Polarization (FP) Fluorescence Polarization measures rotational immobility of a fluorescently labeled compound due to its environment.

  • Page 18: Luminescence

    ‘Lumi Green 1’: wavelength range of 520 to 570 nm • ‘Lumi Blue’: wavelength range of 400 to 515 nm • ‘Lumi Orange’: wavelength range of 550 to 630 nm Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 19: Alphascreen/Alphalisa

    AlphaScreen/AlphaLISA measurements are only possible as endpoint measurements in white or light gray microplates and cannot be performed in combination with the injector system and the heating system. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 20: Software

    Laser Notice No. 50, dated June 24, 2007. Magellan provides measurement data acquisition and customized data reduction for your specific assays. For details, see the Instructions for Use for Magellan. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 21: Installation

    Software (disk or CD-ROM) • Cables (USB 2.0 and main) • Transport lock (mounted) • This Instructions for Use for INFINITE M1000 PRO and the IFU for i-control Each injector module packaging includes the following: • Bottle holder • Beaker for priming •...
  • Page 22 29.5 kg. At least two people must carefully lift the instrument from the box. Caution The maximum load for the INFINITE M1000 PRO cover is 20 kg; STOP however, the load must be distributed evenly across the entire surface of the cover.

  • Page 23: Plate Carrier Transport Lock

    9. The software displays a message stating that the instrument is parked and requests the loosening of the two outer screws from the Transport Lock - confirm with OK. Figure 3 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 24 Save packing materials and transport locks for further transportation STOP purposes. The INFINITE M1000 PRO must be shipped only with the original packing and installed transport locks. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 25: Power Requirements

    USB interface cable or any other cables are connected or removed. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 26: Rear View

    Warning Label: Warning! Switch off the instrument before plugging in or unplugging the module Label: Before shipping the device perform the parking procedure. Use the park device program located in the i-control folder. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 27: Preparing The Infinite M1000 Pro For Shipping

    GUARANTEED. Preparing the INFINITE M1000 PRO for Shipping Before shipping the INFINITE M1000 PRO, the measurement head has to be parked to avoid any damage to the optics and plate transport. This must be performed only by a Tecan service technician; please contact your local Tecan representative.

  • Page 28: Instrument Dimensions

    3. Installation Instrument Dimensions 3.6.1 INFINITE M1000 PRO Instrument Front View Figure 6 Side View Figure 7 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 29: Infinite M1000 Pro Instrument With Built-In Stacker

    3. Installation 3.6.2 INFINITE M1000 PRO Instrument with Built-in Stacker Front View with Built-in Stacker Figure 8 Side View with Built-in Stacker Figure 9 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 30: Injector Module Dimensions

    3. Installation 3.6.3 Injector Module Dimensions Front View Figure 10 Side View Figure 11 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 31: Optical System

    1) the light source system, 2) the fluorescence top optics, 3) the fluorescence bottom optics and 4) the fluorescence detection unit. Fluorescence TOP light Fluorescence BOTTOM light Figure 12: Optical System Fluorescence Top and Bottom 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 32: Light Source System Fluorescence Intensity

    Light Source System Fluorescence Intensity Fluorescence applications usually require a specific range of excitation wavelengths. Additionally, pulsed excitation light may be required (Time Resolved Fluorescence, TRF). The INFINITE M1000 PRO light source system is built from the following components: Flash lamp Condensing optics...
  • Page 33 10 , a value typically achieved by interference filters. In the INFINITE M1000 PRO, a double monochromator is installed on both the excitation and detection side. This allows easy selection of arbitrary excitation and emission wavelengths.

  • Page 34: Fluorescence Top/Bottom Optics

    The emission fibers guide the fluorescence light to the emission monochromator head where a lens system focus the light onto the entrance slit of the emission monochromator. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 35: Fluorescence Intensity Detection

    Excitation Spot Size The size of the fiber bundle cross section determines the diameter of the beam waist (spot size) in the microplate well. The INFINITE M1000 PRO can automatically select between two available orifice diameters depending on the type of microplate required. For microplates up to 384 wells, a spot size of about 2 mm is used.

  • Page 36: Luminescence Scan

    (RLU). The bandwidth can be selected from 5 nm to 20 nm in 1 nm increments. The integration time can be selected from 1 ms to 1 s. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 37: Fluorescence Polarization System

    4.2.1 Light Source System Fluorescence Polarization The polarization light source system is built from the following components: LED light source Lens system Polarization optics Fiber optic bundle 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 38: Fluorescence Polarization Optics

    14). Figure 15 shows the spectra of the resulting light which exits the sample. Blue Green Orange (Blue) (Green) (Orange) (Red) Figure 15: Fluorescence Polarization Excitation light spectra. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 39 (10 – 40 nm) should be used. For each LED an appropriate EX-filter is installed. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 40: Fluorescence Polarization Detection

    However, when using stable samples, a ‘settle time’ can improve FP performance. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 41: Absorbance System

    Before the measurement of the microplate is performed, a reference measurement is made with the plate carrier moved away from the light beam. Figure 17: Optical System Absorbance. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 42: Absorbance Optics

    Measurements using the injector are performed with one absorbance channel only. Figure 19: From left to right: Schematic view of the channel usage of an absorbance measurement of a 96, 384, 1536 and 6-well plate. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 43: Absorbance Detection

    (PMT). The luminescence fiber bundle guides the light from the sample through the filter wheel to the detector. Three different fibers are available for the INFINITE M1000 PRO, the fibers are optimized for different plate types: 96, 384, or 1536-well.

  • Page 44: Luminescence Optics

    The AlphaScreen and AlphaLISA filters installed in the filter wheel can only be used for Alpha measurements, not for luminescence measurements. Figure 21 to Figure 26 show the transmission spectra of the different luminescence filters. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 45 Figure 22: Transmission spectrum of filter ‘Lumi Green’. Wavelength [nm] Figure 23: Transmission spectrum of filter ‘Blue 1‘. Wavelength [nm] Figure 24: Transmission spectrum of filter ‘Green 1‘. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 46: Luminescence Detection

    For best performance, it is recommended to use white plates for luminescence measurements. Note The results of luminescence measurements are always displayed in counts per second, regardless of the integration time used. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 47: Alphascreen/Alphalisa System

    Laser The INFINITE M1000 PRO uses a high power laser (680 nm / 750 mW) as the excitation light source. The INFINITE M1000 PRO is a LASER CLASS 1 product. The INFINITE M1000 PRO complies with FDA radiation performance standards, 21 CFR 1040.10,...
  • Page 48 Figure 28: Transmission spectrum of filter ‘AlphaLISA’. Note The luminescence attenuation and color filters installed in the filter wheel can only be used for luminescence measurements, not for AlphaScreen/AlphaLISA measurements. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 49: Alphascreen/Alphalisa Detection

    To ensure the best performance for AlphaScreen/AlphaLISA assays, the INFINITE M1000 PRO should be operated in a temperature-regulated environment (±1 °C in the range of 20–25 °C). 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 51: Operating The Infinite M1000 Pro

    When operating the INFINITE M1000 PRO always work according to GLP (good laboratory practice) guidelines. Caution The INFINITE M1000 PRO has a fan on the rear of the instrument that STOP draws in air. The air filter must be checked every 4 weeks and must be replaced when dirty.

  • Page 52: General Operating Features

    5. Operating the INFINITE M1000 PRO General Operating Features The INFINITE M1000 PRO has some general behavior and options, which are independent of specifically selected measurement techniques. 5.2.1 Instrument Start Up Before the instrument is switched ON, check if the USB interface cable is connected.

  • Page 53: Finish A Measurement Session

    Usually, wells in the corners evaporate faster. Microplate Shaking The INFINITE M1000 PRO is capable of plate shaking before the start of a measurement or in between kinetic cycles. Three shaking modes are available: linear, orbital and double orbital. The shaking amplitude can be selected from 1 to 6 mm in steps of 0.5 mm for linear and orbital shaking modes, and from 1 to...

  • Page 54: Optimize Fluorescence Measurements

    Excitation spectra are distorted primarily by the wavelength dependence of the intensity of the excitation light the INFINITE M1000 PRO allows you to correct the spectra. To calculate corrected emission spectra, one needs to know the wavelength- dependent efficiency of the detection system.

  • Page 55: Fp Measurements

    By filling in more than one well with polarization references and reference blanks, the mean values will be calculated and therefore the calibration result will be more accurate. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 56 ⎪ ⎪ − ⎩ ⎧ cross − cross ⎪ ⎪ cross − cross ⎪ Δ cross ⎨ ⎪ cross − cross ⎪ ⎪ cross − cross ⎩ Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 57: Instrument Parameters

    “Optimal Gain” setting. Note If any well of interest is assigned “OVER” (overflow), you may manually reduce the gain, or select an automatic gain option (see the software manual). 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 58 1 – 255. The performance of the PMT depends on the supply voltage (see figure below). The INFINITE M1000 PRO PMT is specified from 300 to 1250 V. The relationship between the gain setting of the INFINITE M1000 PRO and the voltage supply is described in the equation below.
  • Page 59 When using the ‘manual’ option, a numeric z-position value can be entered in the measurement stripe. (2) ‘Calculated from well’: When using the option ‘calculated from well’, the INFINITE M1000 PRO will automatically identify the z-position of maximum signal in the selected well for further measurements.
  • Page 60 Upon clicking ‘Apply’, the selected z-position will be automatically applied to i-control script and used for the subsequent measurement. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 61 TRF labels, establish the procedure for the label with the shorter fluorescence lifetime (label 1). Compromise the Integration Time of label 1 with the Lag Time of label 2. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 62: Fi Ratio Mode

    26-30 flashes in a 96-well plate (5 single spots) is performed with 6 flashes per spot, whereas a total flash number of 31 results in 7 flashes per spot. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 63 The standard MRW function for Fluorescence Intensity Bottom reads is disabled when “Optimal Read” is activated and vice-versa. The Optimal Read feature is not available in combination with the gain setting extended dynamic range. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 64: Measurement Accessories

    For low concentrations of TRF labels, white microplates seem superior. You may check if white plates are superior with UV excitation wavelengths. With its z-Positioning capability, the INFINITE M1000 PRO can optimize signal for a particular volume of dispensed sample. However, we do not recommend using volumes less than a third of the maximum volume.

  • Page 65: Absorbance Ratio Mode

    Luminescence PMT may need a certain amount of time to return to the equilibrium baseline count level. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 66: Light Level Attenuation

    Long signal integration combined with very high gain values may result in excessive noise from the detector. The INFINITE M1000 PRO instrument offers a default gain value to keep noise levels low at maximum signal integration. Of course, gain value and integration time may be optimized in assays by the user according to the demands of the application.

  • Page 67: Optimize Alphascreen/Alphalisa

    Note The results of AlphaScreen/AlphaLISA measurements are always displayed in counts per second, regardless of the integration time used. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 68: Injectors

    This label appears on the rear panel of the injector module. Figure 34: View of the injector module. The INFINITE M1000 PRO can be equipped with one pump or two pumps (Figure 34). • Pump A feeds injector needle A •...

  • Page 69: Measurement With Injectors

    5. Operating the INFINITE M1000 PRO 5.7.1 Measurement with Injectors The injectors of the INFINITE M1000 PRO can be used with the following measurement modes: Fluorescence Intensity top and bottom, Time Resolved Fluorescence, Absorbance, Flash and Glow Type Luminescence and Dual-color Luminescence.

  • Page 70: Storage Bottles And Bottle Holders

    The tubes from the injector syringe can be inserted into a carbon needle reaching down to the bottom of the flask to ensure the optimal aspiration of even small volumes of fluid. Figure 36: Bottle holders Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 71: Injector Carrier

    If the injector carrier is not inserted correctly in the injector port, the STOP injector sensor will not detect the inserted injector and therefore washing and priming will be enabled, which can damage the instrument. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 72: Priming And Washing Of The Injector(S)

    Caution Do not touch the injector needles, as they can become easily bent or STOP misaligned, which can cause injection problems or damage the instrument. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 73 Note After priming procedure the syringe remains empty. Caution STOP Close the lid of the pump module (injector box) completely before starting a measurement. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 74 Adjust the parameters on the wash tab of the ‘Injector Maintenance’ dialog box in the ‘Settings’ menu. Start the washing procedure by clicking the ‘Start wash’ button. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 75 Press the button for more than 3 seconds to start ‘prime’. Press the button for less than 3 seconds to start ‘wash’. The parameters have to be set in the i-control software. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 76 ‘injection’ and/or ‘dispense’. Caution STOP It is recommended to empty the waste tub before starting a measurement and to empty it at least once a day. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 77: Injector Modes And Settings (I-Control)

    The option ‘Read time like dispense time’ aligns the measurement time to the dispensing time in order to achieve same reaction times for each measured well. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 78 Therefore, ensure that the selected plate definition file contains the correct value for the working volume. The maximum dispense volume is also limited by the installed syringe size (500 µl; 1 ml; 2.5 ml). Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 79 The Refill volume can be defined for each injector separately and allows economical handling of solutions. Select ‘Refill for every injection’ if refill should be performed for every injection step. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 80 (before and/or after the injection strip) depends on the application. Note Ensure that the correct value for the Working Volume is inserted in the plate definition file. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 81: Injector Cleaning And Maintenance

    These crystals can damage the syringe seal and valve plug resulting in leakage. Caution STOP Do not allow the pump(s) to run dry for more than a few cycles. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 82 10. Clean the end of the injector needles carefully with a cotton swab soaked in 70 % ethanol or isopropanol. 11. For prolonged storage, repeat steps 1-7 with distilled or deionized water and leave the fluid pathways filled for storage. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 83: Injector Reagent Compatibility

    5. Operating the INFINITE M1000 PRO 5.7.7 Injector Reagent Compatibility The injector system of the INFINITE M1000 PRO instruments consists of the following materials: • Teflon (PTFE): Tubing, valve plug, seal • KelF: Valve body • SC05: Injector needles Please refer to the following list for reagent compatibility. Rating ‘A’ indicates a good compatibility with the injector system.

  • Page 84: Built-In Stacker

    (plate height) has been selected in the software before you start the measurement. Maximum plate height is 23 mm. The INFINITE M1000 PRO may be optionally equipped with a built-in stacker for loading and un-loading standard microplates automatically (Figure 42). Only plates without lids can be executed with the stacker.

  • Page 85: Barcode Scanner

    INFINITE M1000 PRO may be optionally equipped with a barcode scanner mounted on the left or right side of the plate transport. INFINITE M1000 PRO is a LASER CLASS 1 product. INFINITE M1000 PRO complies with FDA radiation performance standards, 21 CFR 1040.10, except for deviations pursuant to Laser Notice No.
  • Page 86 Warning The use of controls or adjustments, or the performance of procedures other than those specified herein may result in hazardous radiation exposure. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 87: Instrument Specifications

    The instrument allows for kinetic measurements Reading is not restricted to one part of the microplate, also independent well ranges can be easily selected for measurement at the same time. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 88: Technical Specifications

    -4°F - +140°F Relative humidity Up to 90% (non-condensing) Overvoltage category Pollution degree Usage Commercial Noise level < 60 dBA Electronic waste (infectious waste) Method of disposal Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 89: Fluorescence Intensity And Time Resolved Fluorescence (Trf)

    Fluorescein (Fluorescence Intensity) Top Plate type (number of wells) 1536 Dispensed Volume [µl] Flashes per Well Flash frequency 100 Hz 100 Hz Fluorescein Detection Limit [pM] ≤ 3 ≤ 10 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 90: Fluorescein (Fluorescence Intensity) Bottom

    Lag Time [µs] Flashes per Well Flash frequency 100 Hz Signal/Background-Ratio ≥ 4 Delta F, Low Calibrator [%] ≥ 15 Delta F, High Calibrator [%] ≥ 600 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 91: Fluorescence Polarization

    ≤ ± (1 % + 6 mOD)* Precision (0-2 OD) ≤ ± (0.5 % + 5 mOD)* Linearity (0-2 OD) R2 ≥ 0.999* *Specifications valid only for 384-well plates. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 92: Luminescence

    Luminescence Detection uses the photon counting technique. Parameters Characteristics Wavelength Range 400 – 700 nm Measurement Range > 6 orders of magnitude Integration Time/well 100 – 20000 ms Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 93: Dual-Color Luminescence (E.g. Bret)

    96, 384, and 1536-well microplates Plate Type: (white or light gray) Omnibeads Detection Limit ≤ 12.5 [ng/ml] (384-well) Uniformity [% CV] ≤ 8 (384-well) Crosstalk [%] ≤ 0.2 (384-well) 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 94: On The Fly" Measurements

    ≤ 2 % ≤ 5 % Precision at 450 µl ≤ 0.7 % ≤ 0.7 % ≤ 2 % Injector specifications are valid for 96-well plates only. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 95: Quality Control

    If the results of these control tests do not lie within the official specifications of the instrument, please contact your local service center for further advice. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 96: Definitions

    The accuracy is either calculated as percentage deviation from the true value or for low OD values as the deviation from the true value in mOD. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 97: Crosstalk

    Absorbance Reproducibility (0 – 2 OD) ≤ ± (1 % + 20 mOD) Absorbance Linearity (0 – 2 OD) > 0.999 Absorbance Baseline Flatness (300-700 nm) ± 10 mOD 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 98: Test Instructions

    100 µl pipette (8-channel) + tips 1 nM Fluorescein (diluted in 0.01 M NaOH) 0.01 M NaOH Plate Layout: <> (F….100 µl 1 nM Fluorescein; B…..100 µl 0.01 M NaOH = Blank) Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 99 See chapter 7.4.1.1 Detection Limit Top Fluorescein 384-well Plate Evaluation: Calculate the uniformity: stdev Uniformity mean mean average of wells filled with 1 nM Fluorescein stdev standard deviation of wells 1 nM Fluorescein 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 100 Read Mode: Mode 2 (100 Hz) Number of flashes: Lag time: 0 µs Integration time: 40 µs Plate definition file: GRE1536fb.pdfx, whole plate Z-Position: Calculate from A1 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 101 See chapter 7.4.1.3 Detection Limit Top Fluorescein 1536-well Plate. Evaluation: Calculate the uniformity: stdev Uniformity mean mean average of wells filled with 1 nM Fluorescein stdev standard deviation of wells 1 nM Fluorescein 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 102 20 nm Gain: Optimal Read Mode: Mode 2 (100 Hz) Number of flashes: Lag time: 0 µs Integration time: 40 µs Plate definition file: GRE384fb.pdfx, whole plate Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 103 See chapter 7.4.1.5 Detection Limit Bottom Fluorescein 384-well Plate Evaluation. Calculate the uniformity: stdev Uniformity mean mean average of wells filled with 1 nM Fluorescein stdev standard deviation of wells 1 nM Fluorescein 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 104 − (mean mean mean average of wells filled with 1 nM Fluorescein mean average of wells filled with Blank stdev standard deviation of wells with Blank Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 105 See chapter 7.4.1.7 Detection Limit Bottom Fluorescein 1536-well Plate. Evaluation: Calculate the uniformity: stdev Uniformity mean mean average of wells filled with 1 nM Fluorescein stdev standard deviation of wells 1 nM Fluorescein 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 106: Time Resolved Fluorescence

    − (mean mean mean average of wells filled with 1 nM Europium mean average of wells filled with Blank stdev standard deviation of wells filled with Blank Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 107 − (mean mean mean average of wells filled with 1 nM Europium mean average of wells filled with Blank stdev standard deviation of wells filled with Blank 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 108: Fluorescence Polarization (Fp)

    I3; sample blank same as reference blank Evaluation: Calculate the lower detection limit [mP] = 'precision': FP Precision (mP) = Stdev Stdev Standard deviation of wells filled with Fluorescein (except reference well) Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 109 I5; sample blank is same as reference blank Evaluation: Calculate the lower detection limit [mP] = 'precision': FP Precision (mP) = Stdev Stdev Standard deviation of wells filled with Fluorescein (except reference well) 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 110: Luminescence

    Crosstalk). − − wellx Blank wells A1 – D1 and A3 – D3, respectively ATP wells A2 – D2, respectively wellx Average of wells A4- D10 (Blank) Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 111 Standard deviation of Blank (B: A3 – D9) mean Average of wells filled with ATP standard mean Average of Blank wells (B: A3 – D9) 0.0001 Conversion into mol/well 1/1e Conversion into fmol/well 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 112 Luminescence Crosstalk). − − wellx Blank wells A1 – D1 and A3 – D3, respectively ATP wells A2 – D2, respectively wellx Average of wells A4- D10 (Blank) Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 113 Before pipetting the plate, prepare instrument for measurement: Parameters: Measurement mode: Luminescence Integration time: 1000 ms Plate definition file: GRE1536fw.pdfx Part of the plate: A1 – D10 Start measurement immediately after pipetting! 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 114: Alphascreen

    Prepare 12 further dilutions in 1:2 steps by pipetting 750 µl of the previous dilution step to 750 µl PBS. Use a new tip for each dilution step. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 115 3* stdev of the blank (wells N2-P6) Extrapolate the detection limit [ng/ml] by using the 3-fold standard deviation of the blank as y. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 116 Before pipetting the plate, prepare instrument for measurement: Parameters: Measurement mode: AlphaScreen/AlphaLISA Excitation time: 100 ms Integration time: 300 ms Temperature correction: activated Plate definition file: GRE384fw.pdfx, whole plate Start measurement immediately after pipetting! Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 117 Filling volume: 100 µl/well Omnibeads dilution (2.5 µg/ml) or blank (PBS) Omnibeads dilution (2.5 µg/ml) Blank wells (PBS only) Blank wells used for crosstalk calculation (PBS only) 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 118: Absorbance

    Wavelength step size: 1 nm Number of flashes: Plate definition file: GRE96ft.pdfx Part of the plate: A1 - A1 Evaluation: Calculate the deviation from 0 (unit mOD) Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 119 Number of flashes: Plate definition file: GRE384ft.pdfx Evaluation: Calculate the CV%: stdev Uniformity mean mean average of wells filled with OG stdev standard deviation of well filled with OG 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 120 Calculate the average of each concentration (and blank) and subtract blank from samples. Plot the blank subtracted mean measurement values of Orange G against the concentration values. Calculate the coefficient of correlation (r Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 121 Kinetic interval (Minimal) Evaluation: Calculate the reproducibility (CV%): stdev Reproducib ility(CV%) average average average of well over 20 cycles stdev standard deviation of well over 20 cycles 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 123: Cleaning & Maintenance

    Dispose of contaminated material appropriately. Clean the instrument surfaces with a mild detergent. For biohazardous spills, clean with a 5-10 % solution of bleach in de-ionized water. Wipe cleaned areas dry. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 124: Instrument Decontamination/Disinfection

    STOP and protective clothing. Please note that the decontamination/disinfectant solution can influence the performance of your instrument if applied inside the instrument. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 125: Decontamination/Disinfection Procedure

    11. Wipe dry the outer surface of the instrument with a soft paper towel. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 126: Safety Certificate

    If a Safety Certificate is not supplied, the instrument may not be accepted by the service center. Your local Tecan customer support can send you a new copy of the Safety Certificate, if required. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 127: Disposal

    If you do not intend to keep the packing material for future use, e.g. for transport and storage purposes, return the packaging of the product, spare parts and options via the field service engineer to the manufacturer. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 128: Disposal Of Operating Material

    Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 129: Error Messages And Troubleshooting

    Please report this error to your local Tecan customer support office. Hardware Failure Plate Transport Module ERR: 15 X drive init error Please report this error to your local Tecan customer support office. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 130 Switch instrument off and on, if error persists contact your local Tecan customer support office. Slit steploss. ERR: 62 Slit steploss Switch instrument off and on, if error persists contact your local Tecan customer support office. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 131 Please report this error to your local Tecan customer support office. No plate detection. ERR: 84 Check no plate detect Please report this error to your local Tecan customer support office. 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 132 Error # Error Text Description Emission or excitation busy. ST MEAS HW: ERR: 86 Error 10-99 Please report this error to your local Tecan customer support office. Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...

  • Page 133: Index

    Magellan ............20 Fluorescence Optics ......34, 38 magellan Tracker ..........20 Fluorescence Fiber Bundle ......34 Maintenance ..........123 Fluorescence Intensity Detection ....35 Measurement Techniques ....... 15 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...
  • Page 134 TRF ....See Fluorescence Time Resolved PMT Detector ........... 36 Polarizer ............39 Uniformity ............96 Power Requirements ........25 voltage range ........... 25 Quality Control ..........95 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09...
  • Page 135 4022 Stirrup Creek Road, Suite 310 Toll Free in the US: Durham, NC 27703 Technical support for T +1 800 TECAN US or Tecan instruments T +1 800 832 2687 helpdesk-us@tecan.com 2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0...

  • Page 137: Declaration Of Conformity

    TECAN AUSTRIA GMBH, Untersbergstrasse 1a, A-5082 Grödig / Salzburg, Austria T +43 62 46 89 33, F +43 62 46 72 770, office.austria@tecan.com, www.tecan.com Declaration of Conformity We, TECAN Austria GmbH herewith declare under our sole responsibility that the product identified as: Product Type: Microplate Reader...

Table of Contents

Save PDF