Page 1 ® COULTER STKS Analyzer with Reticulocyte Analysis Operator’ s Guide COULTER STKS PN 4237188B (March 1995) COULTER CORPORATION Miami, Florida 33196...
Page 2 Coulter. Coulter Corporation urges its customers to comply with all national health and safety standards such as the use of barrier protection. This may include, but it is not limited to, protective eye wear, gloves, and suitable laboratory attire when operating or maintaining this or any other automated laboratory analyzer.
Page 3: Table Of Contents
CONTENTS CONTROLS AND INDICATORS, 1 MAIN UNIT, 1 ANALYZER, 2 DILUTER KEYPAD, 4 DMS MONITOR, COMPUTER AND KEYBOARD, 7 POWER SUPPLY, 8 STARTUP, 9 ICONS, 9 CHECK REAGENTS, 9 AT THE DMS, 9 AT THE ANALYZER CRT, 10 AT THE POWER SUPPLY, 12 AT THE DILUTER, 13 CHECK STARTUP RESULTS, 16 DAILY QUALITY CONTROL, 19...
Page 4 CONTENTS Test Modes (Analyzer Conﬁgurations), 47 CBC Mode, 47 CBC/Diff Mode, 48 Retics Mode, 48 WORKFLOW, 48 COLLECTING SPECIMENS, 51 STORING SPECIMENS, 52 PRIMING: WHEN TO PRIME, 53 BAR-CODE LABELS, 54 Rule for Interleaved 2-of-5, 54 PLACING BAR-CODE LABELS, 55 Sample Tubes, 55 Printout Ticket (Auto-Reporter 3), 56 CYCLING SAMPLES, 56...
Page 5 CONTENTS Messages, 82 Population Condition Messages, 83 Suspect Messages, 84 Deﬁnitive Messages, 84 Analyzer Transmit Messages, 86 SHUTDOWN, 89 DAILY SHUTDOWN, 89 PROLONGED SHUTDOWN, 89 AUTOPURGE, 90 ANALYZER CRT FUNCTIONS, 91 CALIBRATION FACTORS, 92 RECEIVE AVERAGE CALIBRATION FACTORS, 93 TRANSMIT CALIBRATION FACTORS, 94 HGB READINGS, 94 START UP TESTS, 95 HGB LAMP ADJUST AND TRANSMIT TO DMS, 96...
Page 6 CONTENTS DMS BASICS, 105 DMS KEYS, 105 MAIN MENU F5-OTHER, 107 STATUS LINE, 108 SET SIZE OF DATA BASE AND WORKLIST, 109 WORKLOAD RECORDING, 109 ACTION LOG, 112 ERROR LOG, 113 RUN SAMPLES DISPLAY, 115 WORKLIST, 121 WORKLIST DESCRIPTION, 121 WHEN YOU PREASSIGN THE WORKLIST, 122 REQUIRED POSITIVE IDENTIFIERS, 123 SETTING UP POSITIVE AND OPTIONAL IDENTIFIERS, 124...
Page 7 CONTENTS DATA BASE QUERY, 137 DATA BASE SORTING, 141 DATA BASE QUERY, RESULTS, 144 EDIT DB SAMPLE, 145 Editing RBC for a Retic Sample, 147 REORDER PATIENT, 147 EDIT PROFILE/DEMOGRAPHICS, 148 COLLATING, 149 Automatic Collation, 151 Manual Collation, 151 Preliminary Report, 152 DATA BASE BATCH PROCESSING, 152 ARCHIVING FROM DATA BASE, 154 To Review Archived Files, 155...
Page 8 CONTENTS QC STATUS, 171 CONTROL ERROR CONDITIONS, 172 CBC/DIFF CONTROL FILE REVIEW, 179 CBC/DIFF CONTROL GRAPHICS DISPLAY, 180 LATEX CONTROL FILE REVIEW, 180 CBC CONTROL FILE REVIEW, 182 RETIC CONTROL FILE REVIEW, 183 BATCH PROCESSING FOR CONTROLS, 183 ¯ X ANALYSIS, 185 MODE TO MODE, 186 APPENDIX A - AUTO-REPORTER 3, 187...
Page 9 BECTON DICKINSON VACUTAINER Tubes, Worldwide, 208 BECTON DICKINSON Glass Tubes with HEMOGARD™ Closure, 208 BECTON DICKINSON Plastic Tubes with HEMOGARD Closure, 208 COULTER, 209 LABO EXPRESS SERVICE (LES), 209 LDM, 209 SHERWOOD MEDICAL, 209 ® TERUMO VENOJECT Tubes, 209 TERUMO VENOJECT II Foil Top, 210...
Page 10 STATUS Messages on Analyzer CRT, 3 When LATRON is Out of Limits, 26 When A Control is Out of Limits, 46 STKS with Retic Analysis Workﬂow Scenarios, 49 CBC/DIFF Parameter Codes, 79 Retic Codes and Messages, 82 Summary of Flagging Messages, 85 Deﬁnitive Flagging Limits, 85...
Page 11: Controls And Indicators
CONTROLS AND INDICATORS MAIN UNIT ANALYZER DILUTER PN 4237188A (December 1993)
Page 12: Analyzer
CONTROLS AND INDICATORS ANALYZER ANALYZER CRT MENU SUMMARY PN 4237188A (December 1993)
Page 13: Status Messages On Analyzer Crt
Occurs when the instrument is ready in the Retics mode. be diluted) RINSE The instrument is rinsing the baths with diluent. STKS INITIALIZING Occurs when you turn the system on and when you press the RESET button on the Analyzer. SYSTEM FAULT The instrument has detected a fault in the system.
Page 14: Diluter Keypad
CONTROLS AND INDICATORS DILUTER KEYPAD Turns on power to the Analyzer and Diluter. POWER When pneumatics are OFF: When pneumatics are ON: PRIME • Activates pneumatics. • Drains and rinses baths. APERT • Backwashes both modes. • Backwashes both modes. •...
Page 15 DILUTER KEYPAD Insert 11 x 17 pages here, pages 5 and 6. Page 6 has a menu summary but no page number on it. PN 4237188A (December 1993)
Page 16 CONTROLS AND INDICATORS PN 4237188A (December 1993)
Page 17: Dms Monitor, Computer And Keyboard
DMS MONITOR, COMPUTER AND KEYBOARD DMS MONITOR, COMPUTER AND KEYBOARD MONITOR REL# 50 100 REL# DF 1 ID#1: 5.29 ID#2: 10.4 53.6 44.0 Data: 01/09/89 38L6 83.1 Time: 10:10:09 31.9 MCHC 41.9 15.2 21.4 COMPUTER F3- Run F4-Print F5- Other F6-DFF7-AP F8-DB F9-Menu MONITOR F1 Help F2-Ticket...
Page 18: Power Supply
CONTROLS AND INDICATORS POWER SUPPLY LINE VOLTAGE POWER SUPPLY MAIN POWER CONTROL VOLTAGE COULTER ELECTRONICS, INC. PN 4237188A (December 1993)
Page 19: Startup
STARTUP ICONS These icons show you how to use keypads of the Analyzer, Diluter and DMS. When you need to use keystrokes, the keys to ANALYZER press follow the icon that shows you which keypad to use. DILUTER The second grouping asks you to press the POWER POWER ON key of the Diluter keypad.
Page 20: At The Analyzer Crt
Operator ID : OPR oooo Check that is set to NONE. AutoPrint oooooooooooo oooooooooooo oooooooooooo OOOOOOOOOOOO CBC/Diff/Retic (931941) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 10/14/93 10 15 DMS↑ PR↓ TK↓ HC↓...
Page 21 AT THE ANALYZER CRT Press in sequence the key next to MAIN SYSTEM CONFIGURATION MODE OF OPERATION Set to CBC+DIFF 7188038A DILUTER CONFIGURATION LEVEL SENSE Check settings. RETURN ALARM Check settings. MAIN MENU 7188031A SYSTEM RUN PN 4237188A (December 1993)
Page 22: At The Power Supply
STARTUP AT THE POWER SUPPLY CAUTION Do not operate the instrument if any indicator is outside the following limits. The pneumatic subsystem has an automatic shutoff feature to prolong the life of the compressor and vacuum pump. If the Diluter is not cycled for about one hour, the pneumatic subsystem turns off. Some error messages can inhibit the time out function.
Page 23: At The Diluter
If VACUUM or 60 psi is out of range, call level. • 60 PSI is 60 ± 5 psi. IN THE USA COULTER • 30 PSI is 30 ± 1 psi. If not, adjust with CUSTOMER OPERATIONS the 30 PSI regulator.
Page 24 STARTUP Verify proper Diluter function. To see better, pull the light bar straight out toward you and lay it aside. DILUTER DRAIN Check that • both aperture baths drain completely. DILUTER RINSE • both baths ﬁll with liquid. • waste chamber drains. LIGHT BAR APERTURE BATHS...
Page 25 AT THE DILUTER DILUTER START for the automatic Start Up cycles. The system: • Converts from cleaning agent to diluent. • Performs a background count in the baths. • Measures Hgb-blank and Hgb-sample voltages. • Performs ramp-pulse and precision- pulse tests. •...
Page 26: Check Startup Results
STARTUP CHECK STARTUP RESULTS Look at the START UP screen when results START UP REAGENT DATES 08/20/93 RAMP TEST 08/20/93 PRECISION TEST 08/20/93 appear on the DMS. Check background Diluent 12/22/93 50.56 63.05 Lyse 11/22/93 5.062 6.384 results. The preset tolerances are: Cleaner 12/22/93 14.20 14.20...
Page 27 ANALYZER MAIN ANALYZER FUNCTIONS STARTUP TESTS BACKGROUND TEST If results are still not acceptable, call Coulter Customer Operations. Return to the System Run display. Check the ramp and precision tests. If any test value exceeds the reference value by 1% or more, the test value appears in red on the DMS screen and is ﬂagged with an H (high)
Page 28 ANALYZER ANALYZER FUNCTIONS START UP TESTS RAMP TEST PRECISION TEST If you see a ﬂag this time, IN THE USA COULTER CUSTOMER OPERATIONS 1-800-526-7694 7188032A Print a copy on the Graphic Printer. Record results in your logbook. PN 4237188A (December 1993)
Page 29: Daily Quality Control
Diff + Retic - F57 Coulter recommends using F57 for monitoring Diff and Retics with the LATRON. The following procedure is for Diff + Retic. If you are using LATRON for Diff only or Retics only, choose the appropriate function. For most efficient reagent use, use the function for what you are going to run.
Page 30 STARTUP Pull open the lower front cover. 7188036A Wipe the aspirator tip thoroughly using distilled water and a lint-free tissue. 7188060A Lift the lower front cover back into place. DILUTER PRIME APERT PN 4237188A (December 1993)
Page 31 Special Functions Control Run Review or Report Graphs Control Batch COULTER ≡≡≡STKS Controls Control Run Press CBC/Diff/Retic (931251) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 05/20/93 10:33 DMS↑ PR↓ TK↓ HC↓ DB↑...
Page 32 STARTUP DILUTER ENTER means the system is ready PRESS MANUAL for you to press the activator. PRESS MANUAL 12. a. Immerse the aspirator tip in the LATRON primer vial. Press and release the activator. DILUTER Message is LATRON DIFF + RET even though you are cycling primer.
Page 33 DO THIS UP TO 3 TIMES COUNT ENTER < 500 2. STEP 12 3. CHECK RESULTS COUNT < 500 CYCLE A NEW VIAL OF PRIMER PRINT COUNT < 500 CALL COULTER CUSTOMER OPERATIONS IN THE USA 1-800-526-7694 CONTINUE PN 4237188A (December 1993)
Page 34 STARTUP Latex DILUTER ENTER PRESS MANUAL to reactivate function 57. 16. Gently mix the control. Invert the vial ﬁve to eight times. Avoid foaming. 7188010A 17. a. Immerse the aspirator tip in the LATRON control vial. Press and release the activator. Remove the control when •...
Page 35 DAILY QUALITY CONTROL 18. When results appear, check for H (High) or DIFF LATEX CONTROL RUN FILE: LATRON RUN: EXP DATE: 03/21/94 L (Low) beside the results and for error LOT: 107359 10/10/93 07:01:34 Mean Channel RESULTS ASSAY DIFF RANGE messages at the bottom of the screen.
Page 36: Retic-C Cell Control
If the control is still "out," set system to CBC mode. Call Coulter Customer Operations for help. RETIC-C Cell Control Retic-C cell control, designed to be used with the COULTER ReticPrep reagent kit, monitors the reticulocyte technology using assessments of volume, conductivity and scatter. IMPORTANT Perform the reticulocyte dilution method according to the instructions on the package insert and below.
Page 37: System Configuration
: On Operator ID : OPR ooooo ooooo oooooooooooo oooooooooooo to turn Prime ON Prime ooooooooo CBC/Diff/Retic (932231) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 08/20/93 12:53 DMS↑ PR↓ TK↓ HC↓ DB↑...
Page 38 F7 Prime mode : Off Operator ID : OPR ooooo ooooo oooooooooooo oooooooooooo to turn Prime off. ooooooooo CBC/Diff/Retic (932231) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 08/20/93 12:53 DMS↑ PR↓ TK↓ HC↓...
Page 39 DAILY QUALITY CONTROL The message on the Diluter keypad is READY..NO BLOOD. READY..NO BLOOD 7188082A Make sure the repipetter is ﬁtted securely to the Reagent B bottle. 7170005A 10. Prime Reagent B, making sure Reagent B ﬁlls the clear tubing of the repipetter. NO BUBBLES HERE 7170006A...
Page 40 STARTUP 11. For each level of control, label two glass test tubes: one for Reagent A and one for Ι Reagent B. Mark the control level on both tubes. 7170001A IMPORTANT Dispensing reagent at an angle changes the dilution of the preparation. 12.
Page 41 DAILY QUALITY CONTROL 15. Incubate for a minimum of 5 minutes at room temperature of 16 - 30°C (61 - 86°F). Up to 60 minutes is OK. 16. Gently mix "A" tube. Aspirate 2 µL of the sample/stain mixture from "A" tube. 2 µ...
Page 42: Run Screen
STARTUP 19. Wait 30 seconds then analyze the prepared sample: Immerse the aspirator tip in the dilution. Press and release the activator. IMPORTANT Do not remove the sample tube from the aspirator tip until the STATUS message on the Analyzer CRT changes from INTRO SAMPLE to 7188037A WIPE, and there is a beep.
Page 43: 5C Cell Control For Cbc/Diff
DAILY QUALITY CONTROL To review the Retic Control ﬁles and graphs, see RETIC CONTROL FILE REVIEW in the CONTROLS chapter. If Retic Control values are out of range, see Table 3, When a Control is Out of Limits. 21. For the next two levels, •...
Page 44: Preliminary Steps
STARTUP Preliminary Steps SEE CONTROLS: NEW LOT NO./ CONTROL FILE FILE FULL SET UP MESSAGE CONTINUE 7188025A 5C cell control is assayed only for the Primary mode. If you use the Secondary mode, your laboratory must determine its own means and expected ranges for each parameter.
Page 45: 5C Cell Control For Cbc/Diff Test In The Primary Mode
: On Operator ID : OPR ooooo ooooo oooooooooooo oooooooooooo to turn Prime on. Prime ooooooooo CBC/Diff/Retic (932231) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 08/20/93 12:53 DMS↑ PR↓ TK↓ HC↓ DB↑...
Page 46 STARTUP When cycling is complete, Other to turn Prime off. IMPORTANT You must properly warm and mix 5C cell control. Follow the procedure on the package insert. To maintain a high read rate, keep the bar-code label free of blood, scratches and powder from gloves. Prepare 5C cell controls according to directions in the package insert.
Page 47 DAILY QUALITY CONTROL Put the cassette in the right-side loading bay. 7188042A Cycle the controls in the Primary mode: DILUTER START /CONT 10. View results on the CBC/DIFF CONTROL CBC/DIFF CONTROL RUN FILE: LOT: 884600 OPR: hs SHIFT : 0 RUN screen.
Page 48: 5C Cell Control For Cbc/Diff In The Secondary Mode
: On Operator ID : OPR ooooo ooooo oooooooooooo oooooooooooo to turn Prime on. Prime ooooooooo CBC/Diff/Retic (932231) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 08/20/93 12:53 DMS↑ PR↓ TK↓ HC↓ DB↑...
Page 49 : Off Operator ID : OPR ooooo ooooo oooooooooooo oooooooooooo to turn Prime off. Prime ooooooooo CBC/Diff/Retic (932231) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 08/20/93 12:53 DMS↑ PR↓ TK↓ HC↓ DB↑...
Page 50 STARTUP Mix controls gently according to directions in the package insert. Perform the 8/8/8 procedure twice. 8 TURNS 8 TURNS 8 INVERSIONS 7022034B Cycle the control: Immerse the aspirator tip in the control vial. Press and release the activator. Remove the control when •...
Page 51: Plus Cell Control And Commercial Cbc-Only Cell Controls
DAILY QUALITY CONTROL 10. If a control is outside limits, the system CBC/DIFF CONTROL RUN FILE: LOT: 884300 OPR: CDS SHIFT : 1 LEVEL: Normal EXP DATE: 10/08/93 RUN: 10/01/93 07:25 RESULTS ASSAY DIFF LIMIT RESULTS ASSAY DIFF LIMIT • stops if AutoStop is on (Status line 5.46 5.44...
Page 52 STARTUP SYSTEM RUN Check that the number of aspirations per tube is set to 1. To turn the AutoStop option ON, see AutoStop in the CONTROLS chapter. 7188089A From the Main Menu, press Other to turn Prime on. Prime Press DILUTER PRIME APERT...
Page 53 DAILY QUALITY CONTROL When the cycle is ﬁnished, Other to turn Prime off. Prime CBC CONTROL RUN FILE: LOT: 085300 OPR: 203 SHIFT : 0 LEVEL: 4C Abn. High EXP DATE: 22/12/93 RUN: 13/10/19 09:34 RESULTS ASSAY DIFF LIMITS +/- 19.2 5.23 0.14...
Page 54 STARTUP Put the cassette in the loading bay. 7188042A DILUTER CBC CONTROL RUN START/ FILE: LOT: 085300 OPR: 203 SHIFT : 0 LEVEL: 4C Abn. High EXP DATE: 22/12/93 RUN: 13/10/19 09:34 CONT RESULTS ASSAY DIFF LIMITS +/- 17.7 17.9 -0.2 5.20 5.19...
Page 55 DAILY QUALITY CONTROL As soon as the screen displays results for the LOW control, change the Control Run ﬁle to the NORMAL for the lot number you are using. Press and select. Repeat the selecting and cycling procedure for HIGH. 12.
Page 56: When A Control Is Out Of Limits
Try another vial or level of control. Be sure to use the correct control ﬁle. Follow directions on the package insert for proper handling. Instrument change Watch for normal sample ﬂow. Call Coulter Customer Operations to help you troubleshoot abnormal operation. PN 4237188A (December 1993)
Page 57: Sample Analysis
Use for LATRON control and primer, with speciﬁc Diluter functions per Test mode. Test Modes (Analyzer Conﬁgurations) Test mode, set at the Analyzer CRT, determines how the data is processed by the STKS. CBC Mode Performs basic CBC on whole-blood samples, in either Primary or Secondary mode.
Page 58: Cbc/Diff Mode
Analyzes prepared Reticulocyte samples in the Secondary mode only. WORKFLOW The STKS with Reticulocyte analysis capability accommodates a variety of workﬂow patterns based on whether or not you elect to preassign samples in the Worklist. You have the option of combining results from the CBC/Diff and Retic test modes on a single collated report.
Page 59: Stks With Retic Analysis Workﬂow Scenarios
IN DATABASE IN DATABASE EDIT CBC OR CBC/DIFF REPORT W/RETIC RESULT (MANUAL ENTRY FROM DATABASE QUERY) Table 4 STKS with Retic Analysis Workﬂow Scenarios Scenario What to do Beneﬁts No DMS Collation, No Preassignment • Retics, CBC/Diff and CBC are Set Analyzer to desired test mode.
Page 60 SAMPLE ANALYSIS Table 4 STKS with Retic Analysis Workﬂow Scenarios Scenario What to do Beneﬁts continued Preassignment, No Collation • Assign Retic Only, CBC Only or Printable Worklist Access Worklist Add. CBC/Diff Only test proﬁle; helps organize results kept separate.
Page 61: Collecting Specimens
WORKFLOW Table 4 STKS with Retic Analysis Workﬂow Scenarios Scenario What to do Beneﬁts continued 4-B. Assign CBC/Diff to Retic existing in Data Base • CBC/Diff test request is added Run Retic. Collation beneﬁts. to existing Retic data. CBC/Diff sample is run, and a new single...
Page 62: Storing Specimens
SAMPLE ANALYSIS You need Primary mode: LEAVE SPACE • minimum of 1.0 mL sample with proper proportion of blood to anticoagulant. • approximately 250 µL of whole blood is aspirated. Secondary mode: 7188046A • 150 µL whole blood is aspirated. •...
Page 63: Priming: When To Prime
STORING SPECIMENS For Reticulocytes 24 HOURS • Run within 8 hours if you store the °C sample at room temperature. °F • Run within 24 hours if you store the sample at 2 to 8°C (35 to 46°F). 7188047A Capillary specimens. Follow the manufacturer’...
Page 64: Bar-Code Labels
11 digits for Interleaved 2-of-5. The bar-code labels used with this system must meet the Coulter speciﬁcations in your Reference manual, PN 4237182. It is important to affix the labels carefully so that the scanner reads them correctly.
Page 65: Placing Bar-Code Labels
PLACING BAR-CODE LABELS PLACING BAR-CODE LABELS Sample Tubes IMPORTANT Place the label as instructed so the scanner can read it correctly. Place the label at the top of the tube, next to the stopper. Place end of label ﬂush with stopper. Press label down securely, including edges and corners.
Page 66: Printout Ticket (Auto-Reporter 3)
SAMPLE ANALYSIS Printout Ticket (Auto-Reporter 3) Place the bar-code label securely within the outline on the ticket. CAUTION Be sure that no label is placed along the sides of the ticket. This area feeds through rollers that do not accommodate the additional thickness. 7188049A CYCLING SAMPLES WARNING...
Page 67 CYCLING SAMPLES SYSTEM CONFIGURATION BLOOD DETECTOR MAIN MENU SYSTEM RUN • CBC↑ or CBC↑ and DIFF↑ If you need to change mode: MAIN SYSTEM CONFIGURATION MODE OF OPERATION Press the key as needed to set test mode. MAIN MENU SYSTEM RUN •...
Page 68 SAMPLE ANALYSIS CAUTION Do not pierce a specimen tube more than ﬁve times. Additional piercing can clog the needle. A VALUE? message appears on the Diluter keypad. DILUTER VALUE? XXXXXXXX ENTER Prime the instrument: DILUTER PRIME APERT Select Sample Analysis Run Samples Other to turn Prime on.
Page 69 CYCLING SAMPLES Slide the normal whole-blood sample into a cassette. Put the cassette into the loading bay on the right side of the instrument. 7188040A DILUTER START/ CONT When cycling is completed Other to turn Prime off. Prime Check that all the F5 Options are correct.
Page 70 SAMPLE ANALYSIS Load the cassettes. IMPORTANT If not using bar-code labels on the sample tubes, it is your responsibility to place the tubes in the proper cassette positions. The recommended operation of this system requires the use of bar-code labels for specimen identiﬁcation in the Primary (cap- piercing) mode.
Page 71: Secondary Mode: Cbc, Cbc + Diff
CYCLING SAMPLES Check for ANALYZER • STATUS: READY • ALERT: (blank) • Sample Run screen DILUTER START/ CONT Verify all sample ID numbers before you 7188096A report results. Secondary Mode: CBC, CBC + DIFF Use the Secondary mode for manual aspiration. Bar-code labels are not read in this mode; you must enter an ID number via the Diluter keypad before cycling the sample.
Page 72 SAMPLE ANALYSIS Check for ANALYZER • STATUS: READY • ALERT: (is blank) • CBC↑ or CBC↑ and DIFF↑ If you need to change mode: MAIN SYSTEM CONFIGURATION MODE OF OPERATION Press the key as needed to set test mode. MAIN MENU SYSTEM RUN Prime the instrument: DILUTER...
Page 73 CYCLING SAMPLES DILUTER ENTER Enter 3 - 11 digits. Immerse the aspirator tip in the sample tube. Press and release the activator. 7188052A IMPORTANT Do not remove the sample tube from the aspirator tip until the STATUS message on the Analyzer CRT changes from INTRO SAMPLE to WIPE , and there is a beep.
Page 74 SAMPLE ANALYSIS Wipe the tip with a lint-free tissue. When cycling is completed Other to turn Prime off. Prime Check that all the F5 Options are correct for your laboratory. To enter the sample ID number: DILUTER WANT GO TO TO ENTER CASS/ STEP C POS NO.
Page 75 CYCLING SAMPLES ALL DIGITS FOR CASS / POS ENTER then enter a 6-digit Cass/Pos number. CASSETTE NO. CASSETTE POSITION 7188061A Enter a 3-11 digit ID#1 for the patient ALL DIGITS FOR ID #1 sample..ENTER 7188062A ANALYZER Check the numbers on the Analyzer CRT to be sure they are correct.
Page 76 SAMPLE ANALYSIS Cycle the sample: Immerse the aspirator tip in the sample tube. Press and release the activator. 7188057A IMPORTANT Do not remove the tube from the aspirator tip until the STATUS message on the Analyzer CRT changes from INTRO SAMPLE to WIPE , and there is a beep. WARNING Be sure to remove the sample tube and be clear of the area around the aspirator tip before the STATUS ﬁeld displays the PRIME message.
Page 77: Secondary Mode: Reticulocytes
Perform the reticulocyte dilution method according to the instructions in the package insert and below. Modiﬁcations to the procedure, or failure to follow these instructions, may lead to misleading or erroneous results. Prepare the sample using the COULTER ReticPrep reagent kit. ANALYZER Set the MODE OF OPERATION to RETICS so that CBC↓...
Page 78 : On Operator ID : OPR ooooo ooooo oooooooooooo oooooooooooo to turn Prime on. Prime ooooooooo CBC/Diff/Retic (932231) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 08/20/93 12:53 DMS↑ PR↓ TK↓ HC↓ DB↑...
Page 79 CYCLING SAMPLES DILUTER ALL DIGITS FOR CASS / POS ENTER and enter a 6-digit Cass/Pos number. CASSETTE NO. CASSETTE POSITION 7188061A Check the numbers on the Analyzer CRT to see if they are correct. Make sure the repipetter is ﬁtted securely to the Reagent B bottle.
Page 80 SAMPLE ANALYSIS 10. Label two glass test tubes for each patient sample: one for Reagent A and one for Reagent B. 7170049A IMPORTANT Dispensing reagent at an angle changes the dilution of the preparation. 11. Holding the dropper bottle vertically, place 4 drops of Reagent A into the "A"...
Page 81 CYCLING SAMPLES 14. Incubate for a minimum of 5 minutes at room temperature of 16 - 30°C (61 - 86°F). Up to 60 minutes is OK. You can incubate samples for up to 60 minutes to allow for batching. Repeat steps 10 through 14 for a batch of samples.
Page 82: Hgb Cuvette Drain
SAMPLE ANALYSIS 17. Place tube "B" at a 30° angle under the tip of the Reagent B dispenser. Dispense 2 mL of Reagent B in the "B" tube. Do not mix. 18. Wait 30 seconds then analyze the prepared sample: Immerse the aspirator tip in the dilution.
Page 83 CYCLING SAMPLES Secondary mode Approximately 15 minutes after the last CBC or CBC/Diff sample is run, and no sample has been run in the Primary mode. The drain and rinse routine consists of these functions: • The Hgb cuvette drains into the waste chamber. •...
Page 84 SAMPLE ANALYSIS PN 4237188A (December 1993)
Page 85: Data Analysis
DATA ANALYSIS CBC HISTOGRAMS IMPORTANT Do not estimate the number of cells from the distribution curves. Curves show only the relative, not actual, number of cells in each size range. RBC Distribution Curve The normal RBC curve ranges from 36 to 360 fL. The display starts at 24 fL.
Page 86: Diff Scatterplots And Histograms
DATA ANALYSIS DIFF SCATTERPLOTS AND HISTOGRAMS STKS diff reagents maintain white cells in their near native state. The STKS looks at cells in all three dimensions. You see results on your screen • two dimensions at a time • in three different views.
Page 87: Df 2 Scatterplot
DIFF SCATTERPLOTS AND HISTOGRAMS DF 2 Scatterplot DF 2 is derived primarily from conductivity. From this perspective, lymphocytes, monocytes and granulocytes are the prominent populations. The granulocytes include the neutrophils, basophils and eosinophils. DF 3 Scatterplot DF 3 displays the same data as DF 2 with the neutrophil and eosinophil populations gated out.
Page 88: Single Parameter Histograms
DATA ANALYSIS Single Parameter Histograms Single parameter histograms of volume, conductivity and light scatter are also available for Diff and Retics. These are used primarily for troubleshooting by Service. RETICULOCYTE SCATTERPLOTS DF 5 Scatterplot This is the initial display of Retic analysis. It derives primarily from light scatter and volume.
Page 89: Df 6 Scatterplot
RETICULOCYTE SCATTERPLOTS DF 6 Scatterplot then rotates the cube. You cannot readily see Retics from this screen as they appear in front of the RBC population. PARAMETER REVIEW Precision of Results Results, and the calculations performed on those results, appear as a preset number of decimal places on the screen.
Page 90 DATA ANALYSIS Table 5 CBC/DIFF Parameter Codes On DMS Display Cause continued "-----" for CBC parameter results, and no average Total voteout (at least 2 out of 3 measurements do histogram for the affected parameter not agree). Diff parameters do not vote out. If WBC is "-----", then LY#, MO#, NE#, EO#, and BA# are ".."...
Page 91 PARAMETER REVIEW Table 5 CBC/DIFF Parameter Codes On DMS Display Cause continued "+++++" for parameter results (con’t) IMPORTANT Sample dilutions may result in erroneous differential results. When WBC is overrange, if you dilute the sample to rerun it, be sure to set the instrument to CBC↑ before you cycle the diluted sample.
Page 92: Reticulocyte Codes
DATA ANALYSIS Reticulocyte Codes If any of the following ﬂags occur, review results for the affected parameter(s) according to your laboratory’ s protocol. Table 6 Retic Codes and Messages On DMS Display Cause Message in Condition Box ::::: FC under Scatterplot Full clog VERIFY RETIC ..
Page 93: Population Condition Messages
PARAMETER REVIEW SUSPECT/ DEFINITIVE FLAG WINDOW FLOW CELL CLOG MESSAGES TRANSMIT MESSAGES APPEAR HERE POPULATION CONDITION MESSAGES 7188097A Population Condition Messages WBC, RBC, Plt, Retic Condition messages describe the WBC (including Differential, if performed), RBC and Plt populations. These messages occur in the lower left corner of the Run Samples screen. They indicate the WBC, RBC, Plt and Retic population status only.
Page 94: Suspect Messages
DATA ANALYSIS The differential count for all parameters, when totaled, equals 100% except when ALL differential parameters appear as zeros. If the differential count displays all zeros, check for additional ﬂagging and take appropriate action according to your laboratory’ s protocol. Verify Retic Verify Retic is a comprehensive message that appears at the bottom of the population message window.
Page 95: Summary Of Flagging Messages
PARAMETER REVIEW Table 7 Summary of Flagging Messages Parameter Condition Suspect Deﬁnitive Normal WBC Pop Blasts Leukopenia Abnormal WBC Pop Imm Grans/Bands 1 Leukocytosis Edited Data Imm Grans/Bands 2 Neutropenia No message Variant Lymphs Neutrophilia Review Slide Lymphopenia Lymphocytosis Monocytosis Eosinophilia Basophilia Normal RBC Pop...
Page 96: Analyzer Transmit Messages
DATA ANALYSIS Table 8 Deﬁnitive Flagging Limits For this Flag This Message Indicates the Result Exceeds this Limit continued Anemia Low limit for RBC or for Hgb 1+ Anisocytosis High limit for RDW 2+ Anisocytosis A gradient range from 1+ Anisocytosis 3+ Anisocytosis A gradient range from 2+ Anisocytosis 1+ Microcytosis...
Page 97: Transmit Messages
PARAMETER REVIEW Table 9 Transmit Messages Message Description Xmit DMS Appears when Primary mode results are transmitted manually from the Analyzer via TRANSMIT TO DMS. SXmit DMS Appears when Secondary mode results are transmitted manually from the Analyzer via TRANSMIT TO DMS. Ramp Appears when a Ramp test is sent from the Analyzer.
Page 98 DATA ANALYSIS PN 4237188A (December 1993)
Page 99: Shutdown
At the end of the cycle, the pneumatic subsystem automatically shuts down. PROLONGED SHUTDOWN Perform the following Shutdown procedure if you turn off the power at night, and the STKS is going to be idle for more than 48 hours.
Page 100: Autopurge
SHUTDOWN Perform and verify Quality Control checks per your laboratory’ s protocol. Operate as usual. AUTOPURGE If 24 hours elapse with the power on, the pneumatics off, and the instrument in shut down, the system automatically: • Turns on the pneumatics. •...
Page 101: Analyzer Crt Functions
ANALYZER CRT FUNCTIONS This chapter describes the Analyzer CRT functions, in the order they appear on the MAIN MENU: ANALYZER FUNCTIONS SYSTEM CONFIGURATION TURN PNEUMATICS OFF SYSTEM RUN Each function is illustrated by a series of screens. The black key indicates which key to press to access the next screen.
Page 102: Calibration Factors
ANALYZER CRT FUNCTIONS CALIBRATION FACTORS Key Sequence: 1,1,1,5 This function displays • the average (AVG), *MAIN MENU* ANALYZER FUNCTIONS SYSTEM CONFIGURATION • aperture 2 (AP2), and TURN PNEUMATICS OFF CYCLE COUNTER: XXXXXXX • aperture 3 (AP3) calibration factors *ANALYZER FUNCTIONS* CALIBRATION HGB READINGS for WBC, RBC, Hgb, MCV, Plt, and MPV.
Page 103: Receive Average Calibration Factors
RECEIVE AVERAGE CALIBRATION FACTORS RECEIVE AVERAGE CALIBRATION FACTORS Key Sequence: 1,1,2 This function lets the Analyzer receive calibration factors from the DMS calibration option. *MAIN MENU* ANALYZER FUNCTIONS SYSTEM CONFIGURATION Press the key next to this function, then transmit TURN PNEUMATICS OFF the factors from the DMS.
Page 104: Transmit Calibration Factors
ANALYZER CRT FUNCTIONS TRANSMIT CALIBRATION FACTORS Key Sequence: 1,1,3 and 1,1,4 Use these functions as indicated to transmit from the Analyzer to the DMS, either the average calibration factors, or the average and individual *MAIN MENU* ANALYZER FUNCTIONS aperture factors. SYSTEM CONFIGURATION TURN PNEUMATICS OFF •...
Page 105: Start Up Tests
START UP TESTS START UP TESTS Key Sequence: 1,3 Use this function to perform the ramp, precision and background tests. The background test includes background, Hgb voltages and diff test. *MAIN MENU* When you press the key, the system ANALYZER FUNCTIONS SYSTEM CONFIGURATION automatically performs the test and sends results TURN PNEUMATICS OFF...
Page 106: Hgb Lamp Adjust And Transmit To Dms
ANALYZER CRT FUNCTIONS HGB LAMP ADJUST AND TRANSMIT TO DMS Key Sequence: 1,4 and 1,5 HGB LAMP ADJUST *MAIN MENU* Use this function, with the pneumatics on and ANALYZER FUNCTIONS SYSTEM CONFIGURATION diluent (not cleaning agent) in the baths, to TURN PNEUMATICS OFF check the Hgb lamp voltage.
Page 107: Set Date Format
SET DATE FORMAT SET DATE FORMAT Key Sequence: 1,6,1 Use this function to select the date format. Press the key next to DATE FORMAT to toggle *MAIN MENU* between these choices: ANALYZER FUNCTIONS SYSTEM CONFIGURATION TURN PNEUMATICS OFF U.S. MM/DD/YY EUROPEAN DD/MM/YY CYCLE COUNTER: XXXXXXX JAPANESE...
Page 108: Set Time And Date
ANALYZER CRT FUNCTIONS SET TIME AND DATE Key Sequence: 1,6,2 Use this function to set the time and the date. On the Diluter keypad, enter the correct values for the month, day, year, hours, minutes and *MAIN MENU* seconds, then press after each entry.
Page 109: Diluter Configuration, Level Sense Settings
DILUTER CONFIGURATION, LEVEL SENSE SETTINGS DILUTER CONFIGURATION, LEVEL SENSE SETTINGS Key Sequence: 2,1,1 Use this function to enable or disable the level sensors. Press the key next to the option to change the setting. PN 4237188A (December 1993)
Page 110: Diluter Configuration, Alarm Settings
ANALYZER CRT FUNCTIONS DILUTER CONFIGURATION, ALARM SETTINGS Key Sequence: 2,1,2 Use this function to enable or disable the alarms. Press the key next to the option to change the setting. *MAIN MENU* ANALYZER FUNCTIONS SYSTEM CONFIGURATION TURN PNEUMATICS OFF *SYSTEM CONFIGURATION* CYCLE COUNTER: XXXXXXX DILUTER CONFIGURATION NUMBER OF BAR CODE DIGITS (2 OF 5) 11...
Page 111: Other System Options
OTHER SYSTEM OPTIONS OTHER SYSTEM OPTIONS Key Sequences: 2,3; 2,4 In addition to setting the Diluter conﬁguration and the number of bar code digits, use this function to: *MAIN MENU* ANALYZER FUNCTIONS • Enable or disable the blood detector. SYSTEM CONFIGURATION TURN PNEUMATICS OFF •...
Page 112: System Run
ANALYZE DIFF RETICS 7182006A WIPE (Secondary mode) BACKWASH SYSTEM FAULT ACTIVE (Primary mode) READ HGB STKS INITIALIZING PRIME RINSE AUTOCLEARING CHECKING PRESSURES READY (Retics must be diluted) COUNT COMPLETED • ALERT messages inform you of conditions that may warrant attention. These messages are described in the Special Procedures and Troubleshooting manual, PN 4237187.
Page 113: Analyzer Crt Pulses
SYSTEM RUN Analyzer CRT Pulses SYSTEM RUN Some STKS instruments have an enhanced Analyzer CRT. This CRT displays pulses during the Count portion of each cycle. Use the pulse displays to monitor the condition of all the apertures. You can detect noise, bubbles, or a clogged ﬂow cell.
Page 114 ANALYZER CRT FUNCTIONS ANALYZER CRT MENU SUMMARY CALIBRATION FACTORS RECEIVE AVERAGE CALIBRATION FACTORS TRANSMIT AVERAGE CALIBRATION FACTORS CALIBRATION TRANSMIT ALL CALIBRATION FACTORS MAIN MENU RETURN ANALYZER FUNCTIONS HGB READINGS (BLANK MAIN MENU & SAMPLE VOLTAGES) RETURN RAMP TEST PRECISION TEST START UP TESTS BACKGROUND TEST...
Page 115: Dms Basics
DMS BASICS This chapter includes DMS Keys Main Menu F5-Other Status Line Set Size of Data Base and Worklist Workload Recording Action Log Error Log DMS KEYS This list gives a general deﬁnition of the keyboard. It does not include function keys that are speciﬁc to individual screens.
Page 116 DMS BASICS Function Goes to the previous menu and saves entered data. Moves one page up in a Help ﬁle or a scrollable region. Moves one page down in a Help ﬁle or a scrollable region. DISPLAYING SCREENS AND MESSAGES Displays a choice list if one is attached to the ﬁeld you are on.
Page 117: Main Menu F5-Other
: OPR ooooo ooooo oooooooooooo oooooooooooo ooooooooo • NONE - do not print any results. CBC/Diff/Retic (933083) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu • ALL - print all results. 12/03/93 11:21 DMS↑...
Page 118: Status Line
DMS BASICS STATUS LINE The Status line at the bottom of the screen shows the current date, time and operator identiﬁcation, and indicates the current status of the various options listed. Background color changes indicate certain conditions: red may require immediate operator attention, yellow indicates busy.
Page 119: Set Size Of Data Base And Worklist
Database size set up Number of samples in database: 1000 Supervisor Password ﬁrst delete the Worklist. Number of entries in Worklist: CBC/Diff/Retic (931582) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu • The number of records in the Worklist 06/09/93 09 00 DMS↑...
Page 120 DMS BASICS Each graph is color coded: Color Primary Secondary Non-Patient Green CBC-DIFF CBC-DIFF CBC-CAL CBC-CTRL Lt Magenta C/D-CTRL Blue RETICS LATRON Magenta RET-CTRL Yellow OTHERS: CARRYOVER REPRODUCIBILITY STARTUP BACKGROUND PRIME SERVICE TESTS The Workload Recording operation logs data for up to •...
Page 121 WORKLOAD RECORDING • restarts the accumulation process When it reaches 365 days of accumulation, this message appears on the Error line and is posted to the Error Log: WORKLOAD - MAXIMUM DAYS REACHED - ACCUMULATION RESTARTED When it reaches a count of 1166400, this message appears on the Error line and is posted to the Error Log: WORKLOAD - MAXIMUM COUNT...
Page 122: Action Log
Operations. Reset Analyzer. Problem solved. instrument serial number to each entry. Page 0 of 150 (931941) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F2-Stamp F3-Print_Pg F4-Print F5-Other F6-Del F7-Archive PgUp/PgDown Saves and prints the page that Print Pg 10/27/93 10:00 DMS↑...
Page 123: Error Log
ERROR LOG ERROR ERROR LOG When an error message occurs on the DMS, it is Error Log 03/16/93 10:24:34 m2_3_602.c375: Error on Read recorded in the Error Log. When you run a batch 07/09/93 10:53:25 No space available to archive CONTROL ﬁle in Drive F of samples, you can use the Error Log to see 07/15/93 09:44:14 No space available to archive CONTROL ﬁle in Drive F 09/04/93 13:38:33 Unable to archive CONTROL ﬁle : File1.wks...
Page 124 DMS BASICS PN 4237188A (December 1993)
Page 125: Run Samples Display
RUN SAMPLES DISPLAY Sample Analysis Run Samples The DMS screen displays the sample results. The display includes scatterplot, histograms, parameter data, and ﬂagging codes and messages as they apply. This example shows the CBC and Diff results. PN 4237188A (December 1993)
Page 126 RUN SAMPLES DISPLAY On a collated sample, press to view the CBC and Retic results. Press to view the CBC and Diff results again. RETIC RESULTS 7188094A Function keys available: Provides additional information Help about the Sample Analysis screen. Generates a ticket printout. The Ticket AutoReporter 3 does not print Retic results.
Page 127 RUN SAMPLES DISPLAY Use this option to set the Other conditions that appear in the Status line. When you press , another window appears; it includes: Provides additional Help information about this window. Turns ¯ X Analysis on and off. Also displays the number of samples run so far in the current batch, and indicates...
Page 128 RUN SAMPLES DISPLAY IMPORTANT When AutoPrint is ON (set to ALL, ABNORMALS or NORMALS) and PR↑ appears on the Status line, these options are not functional: B&W Screen Print Color Screen Print (continued) Other AutoTransmit Turns automatic transmission to host computer on and off. Applies to Sample Analysis and Controls.
Page 129 RUN SAMPLES DISPLAY Use to look at samples stored in the Data Base. Returns display directly to the Menu Main Menu. CD or CR Use to toggle between CBC/DIFF results and CBC/RETIC results on a collated sample. PN 4237188A (December 1993)
Page 130 RUN SAMPLES DISPLAY PN 4237188A (December 1993)
Page 131: Worklist
WORKLIST This chapter includes: Worklist Description When You Preassign the Worklist Required Positive Identiﬁers Setting up Positive and Optional Identiﬁers Setting up Physician and Location Lists Auto Sequencing on the Worklist To Add an Entry to the Worklist To Edit a Worklist entry Worklist status: Collate Failed When You do Not Preassign the Worklist Primary Mode...
Page 132: When You Preassign The Worklist
WORKLIST Print entries that are tagged on Print Worklist screen displayed. Print all tests for the entries tagged on Worklist screen displayed. Key strokes active, but not seen on screen. Set automatic sequencing ON/OFF . Delete entries that are tagged on Worklist.
Page 133: Required Positive Identifiers
WHEN YOU PREASSIGN THE WORKLIST directly to the Data Base. This situation does not generate a NO MATCH message. After each test is successfully completed, it is Worklist Current line Entries Tagged removed from the Worklist for that test. If other <...
Page 134: Setting Up Positive And Optional Identifiers
WORKLIST If the system is set for a single Positive Identiﬁer (Cass/Pos or ID#1), and you preassign both IDs, it checks for both. If it does not ﬁnd a match for the non-Positive Identiﬁer, it generates an ID MISMATCH message. SETTING UP POSITIVE AND OPTIONAL IDENTIFIERS IMPORTANT There must be no data in the Data Base, the Worklist, or the Host Worklist when you set up or change...
Page 135: Setting Up Location And Physician Lists
SETTING UP POSITIVE AND OPTIONAL IDENTIFIERS You can also set up a group of optional identiﬁers. The default options are: ID#2, Sequence number, Status, Date of birth, Sex, Location, Physician, Date and Time, User ﬁeld 1, User ﬁeld 2, User ﬁeld 3, and Comments. You can change any of these labels to suit your laboratory’...
Page 136: Automatic Sequencing On The Worklist
WORKLIST AUTOMATIC SEQUENCING ON THE WORKLIST When you go to the Worklist, there are three ﬁelds you can set to sequence automatically: Cass/Pos ID#1 Sequence # To go to the Worklist Auto-sequencing window, Worklist Current line Entries Tagged < CBC/DIFF > Cass/pos ID# 1 ID# 2...
Page 137: To Add An Entry To The Worklist
AUTOMATIC SEQUENCING ON THE WORKLIST When you want to set or change any of the numbers that sequence automatically, place the cursor in the appropriate ﬁeld and type in the new sequence number. The system picks up at the newly set number and begins the sequence from there.
Page 138 WORKLIST • Change any sequence number. Auto- sequencing starts with new number. Press to save your entries and return to Sample preassignment window. Enter ID numbers. You must enter either a Worklist Current line Entries Tagged Cass/Pos or ID# 1 for each test. You can <...
Page 139 TO ADD AN ENTRY TO THE WORKLIST: • press for the Location list. Move Worklist Current line Emergency Tagged the cursor to the location you want and Maternity RETIC > Cass/pos Intensive Care Sequence # Tests Status 1st ﬂoor east press 001208 0630...
Page 140: To Edit A Worklist Entry
WORKLIST 11. Enter any other patient identiﬁcation data, Worklist Current line Entries Tagged including comments, then < RETIC > Cass/pos ID# 1 ID# 2 Sequence # Tests Status 001208 063037 R Rooney 100014 Sample preassignment Proﬁle# 3 • press to save and go to a blank Proﬁles 1 2 3 4 5 6 7 8 9 Cass/pos ID# 1 Status...
Page 141: Worklist Status: Collate Failed And Partial Aspiration
WORKLIST STATUS: COLLATE FAILED AND PARTIAL ASPIRATION WORKLIST STATUS: COLLATE FAILED AND PARTIAL ASPIRATION COLLATE FAILED If a collate failure occurs: • A COLLATE FAILED message appears in the Worklist Status ﬁeld and in the Run Samples status ﬁeld. • The test that failed collation is saved to Data Base as a separate entry.
Page 142: No Read, No Match, Id Mismatch
WORKLIST • The Ticket printout is not automatically printed. • The results are not automatically transmitted to the Host computer if the PART.ASP/NO READ option is set to NO. However, if the PART.ASP/NO READ option is set to YES, and if AutoTransmit is on, and a PART.ASP occurs, then the system transmits all sample information, including identiﬁers, the Partial Aspiration message and dots (..) for...
Page 143 NO READ, NO MATCH, ID MISMATCH NO READ If a NO READ occurs: ID#1 is a Positive Identiﬁer and the bar-code • A NO READ message is posted to the Error reader could not read the tube label. Log. Example: "NO READ: Sample run received without proper identiﬁcation.
Page 144 WORKLIST Example: "NO MATCH: Sample run received does not match any Worklist entry (<Cass/Pos>, <ID# 1>)." • If Autoprint is ON (that is, set to ALL, NORMALS or ABNORMALS), the Graphic Printer prints the sample identiﬁcation and the NO MATCH message. Parameter labels and data do not print.
Page 145: Message Summary
NO READ, NO MATCH, ID MISMATCH • Sample results and identiﬁers can be edited in Data Base Query. Message Summary The priority of messages in the Worklist and Data Base is as follows: Data Base Query Flags ﬁeld Data Base Status ﬁeld Worklist Status ﬁeld No Read No Read...
Page 146: Host Worklist
WORKLIST The error posted to the Error Log might be: "Operator acknowledged Worklist system stop errors." Press again to clear the error line. HOST WORKLIST If your system is connected to a host computer, HOST WORKLIST Total # of samples you can transfer sample identiﬁcation data from No.
Page 147: Data Base
Special Functions. After the maximum number of samples, wraparound occurs and the oldest CBC/Diff/Retic (931483) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED (determined by time of last test analyzed in a F1-Help F3-Error Log...
Page 148 DATA BASE When you access this option, you see what was Data Base Query Saved Entries Tagged selected by the last Sort criteria. To review other Cass/pos ID# 1 Date Time ID 2 Tests Flags samples, change the Sort criteria. If the last 001601 ----------- 08/06/93 14:01:18 OPR 001601 -----------...
Page 149 DATA BASE QUERY • Flags ﬁeld: Blank means not ﬂagged F means ﬂagged These symbols also appear in the Flags ﬁeld: NM means NO MATCH NR means NO READ CF means COLLATE FAILED PA means Partial Aspiration • Output: A = Archive P = Batch Print H = Host S = Save...
Page 150 DATA BASE Goes directly to Edit demog./ proﬁle window instead of going to ﬁrst. Key strokes active, Edit but not seen on screen. Print sorted entries displayed on Print the Data Base Query screen. Print, transmit or archive by Batch batches;...
Page 151: Data Base Sorting
DATA BASE QUERY Toggle display between groups of Next parameter results. These messages might appear at the bottom of the screen. RESULTS NOT AVAILABLE - It means results are not stored in the data base. They may have been overwritten when wraparound occurred, or they were never received in the data base.
Page 152 DATA BASE When sorting ID#1 or ID#2, enter the full name or identiﬁer to start from and go to. To sort the full alphabet, enter from A to ZZ. Make all numeric identiﬁcations the same length; if a numeric ID is shorter than other IDs in the data base, enter leading zeros to make it the same length.
Page 153 DATA BASE SORTING Function keys available: Help Reset a ﬁeld to its previous setting. Reset Use before executing the sort, or after clearing a ﬁeld to blank by pressing Clear a ﬁeld to blank. A blank ﬁeld Clear indicates no limits; that is, that all entries for that criterion will be included.
Page 154: Data Base Query, Results
DATA BASE DATA BASE QUERY, RESULTS Sample Analysis Data Base Query To view parameter results from the Data Base Data Base Query Saved Entries Tagged Query screen, press . The list in this Cass/pos ID# 1 Date Time ID 2 Tests Flags example shows these proﬁles: 002201 100.
Page 155: Edit Db Sample
DATA BASE QUERY, RESULTS Press again to view: Data Base Query Saved Entries Tagged Cass/pos ID# 1 Time RBC, MCH, RDW, MPV 002201 100. 10:51 3.74 L 23.5 L 14.8 002202 ----------- 10:52 3.69 L 24.1 L 15.0 002206 ----------- 10:52 4.35 30.4...
Page 156 DATA BASE Press to go back to EDIT DB SAMPLE screen. You can edit all ﬁelds except the selected Positive Identiﬁer, the date and time the sample was processed, and any messages in the status ﬁeld. If a ﬁeld is edited, E appears in the ﬁeld in all EDIT DB SAMPLE Cass/pos ID# 1...
Page 157: Editing Rbc For A Retic Sample
EDIT DB SAMPLE • The EDITED DATA message appears on the Graphic printout. • E is printed on the Graphic printout and on the ticket next to edited result. • Data transmitted to a host computer includes the EDITED DATA message and the E ﬂag(s).
Page 158: Edit Profile/Demographics
DATA BASE You can use to check the Auto-sequence settings. If Auto-sequencing is ON for the indicated test, you can assign a sequence number by pressing This sample is posted to the Worklist and is treated like a separate entry. EDIT PROFILE/DEMOGRAPHICS Sample Analysis Data Base Query...
Page 159: Collating
COLLATING COLLATING Collating is the process of combining the results of two or more runs of the same sample in different modes. You can view, print and transmit collated reports. This is an example of a collated printout that includes graphics. Requirements for Collation For collating to occur, these requirements must be met.
Page 160 DATA BASE The following conditions cause a COLLATE FAILED message to appear on the Worklist, a CF message to appear in the Data Base Query Flags ﬁeld, and a Collate failed because..message to be posted to the Error Log. These messages are described in the Special Procedures and Troubleshooting manual, PN 4237187.
Page 161: Automatic Collation
COLLATING Automatic Collation • Preassign CBC/Diff/Retic proﬁle on the Worklist before either test performed. • Preassign Retic after doing CBC/Diff or vice versa Sort CBC/Diff or Retic from Data Base Query. Select sample on Data Base Query list. Press Edit demog./proﬁle. Change proﬁle to include test to be collated.
Page 162: Preliminary Report
DATA BASE Preliminary Report If you print a report before both preassigned tests have been run, the report gives the results for the completed test and a PRELIMINARY REPORT message. DATA BASE BATCH PROCESSING IMPORTANT Do not cycle the instrument while batch processing. If you do, the instrument stops. Sample Analysis Data Base Query Batch...
Page 163 DATA BASE BATCH PROCESSING Data that is transmitted to a host computer goes through a buffer called a spooler. If the spooler is enabled, and the host computer is not ready to receive, the spooler holds up to 1000 samples until the host computer is ready.
Page 164: Archiving From Data Base
DATA BASE ARCHIVING FROM DATA BASE Sample Analysis Data Base Query Sort The DMS Archive feature lets you copy data from Data Base Query Saved Entries Tagged the DMS and retrieve it on another computer in a Cass/pos ID# 1 Date Time ID 2...
Page 165: To Review Archived Files
ARCHIVING FROM DATA BASE Move cursor to Filename ﬁeld and enter the ﬁle name. Use up to eight characters plus a .XXX extension. If you do not enter an extension, the system automatically adds a .WKS extension. Example: 23June93.WKS Insert a formatted disk in the DMS disk drive.
Page 166 DATA BASE The key to these codes is: Some of the columns use one-character codes to represent what is in them. • In the C/P Edit and ID1 Edit ﬁelds: Example: 0 = not edited Cass/Pos C/P Edit ID#1 ID1 Edit 002201 063037 1 = edited...
Page 167: Delete Data Base
DELETE DATA BASE DELETE DATA BASE When you delete the data base, the last sample Special Functions Set Up run remains in the buffer, and is displayed when Sample Analysis you go to the Run Sample screen. The buffer sample disappears after the next cycle occurs. When you delete the data base, samples that are pending in the host spooler are not deleted.
Page 168 DATA BASE PN 4237188A (December 1993)
Page 169: Controls
CBC/DIFF Files oooooooooooo CBC Files oooooooooooo Retic Files oooooooooo Normal Abnormal I Abnormal II CBC/Diff/Retic (932422) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 09/04/93 09 01 DMS↑ PR↑ TK↑ HC↑ DB↑...
Page 170: Set Auto-Stop Option
ﬁle. Then when you set up the control MCHC iles ﬁle, these ranges are automatically posted to the 0.030 ﬁle according to the coded lot numbers. CBC/Diff/Retic (932422) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F4-Print F9-Menu F10-Save/Esc 09/04/93 14 17 DMS↑...
Page 171: Iqap Id Set Up
IQAP ID # 63037-1-G1-2 Supervisor Password Screen Saver Time Out To receive a separate report for each shift, the CBC/Diff/Retic (932422) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F4-Print F6-Clear F10-Save/Esc IQAP number for shifts two and three would 09/04/93 09 10 DMS↑...
Page 172: Control Management By Shift
24 hours automatically. 07:00 - 14:59 nication def ronic reference 15:00 - 22:59 ID # ooooooo 23:00 - 06:59 CBC/Diff/Retic (931251) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F4-Print F10-Save/Esc 06/01/93 08 50 PN 4237188A (December 1993)
Page 173: Control File Set Up
Retic Files Expected Range oooooo Auto Stop oooooo oooooooooooo oooooooooooo oooooooooooo CBC/Diff/Retic (931483) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 06/05/93 08:34 Select the type of control ﬁle you want and press...
Page 174 CONTROLS The cursor is in the LOT ﬁeld. Enter the lot CBC/DIFF CONTROL SET UP FILE : 12 LOT: 883126 IQAP ID # 63037-5-27-3 number and press . With 5C cell control, LEVEL: Normal EXP DATE: SHIFT: HOST : OFF OPR: the system automatically enters the level and the Parameter...
Page 175 CBC/DIFF FILE SETUP If your system includes a bar-code wand, you can enter the setup data for 5C cell control by passing the wand over the bar-coded ﬁelds of the assay sheet. Be sure to enter ﬁeld number one, Lot number, ﬁrst.
Page 176: Latex File Set Up
ﬁrst ﬁle that is not set up. Latex Files F55-DIFF only Press F56-Retic only oo F57-DIFF + Retic oooooooooooo oooooooooooo oooooooooooo CBC/Diff/Retic (931483) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 06/05/93 12 00 PN 4237188A (December 1993)
Page 177: Cbc File Set Up
ﬁle. CBC Files Retic Files Expected Range Auto Stop oooooooooooo oooooooooooo oooooooooooo oooooooooooo ooooooooooo CBC/Diff/Retic (932231) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 08/24/93 14 58 DMS↑ PR↑ TK↑ HC↑ DB↑...
Page 178 CONTROLS You can adjust the Expected Range by moving the cursor to the appropriate ﬁeld and entering new numbers. The LEVEL ﬁeld was automatically ﬁlled in when you entered the lot number. When you move the cursor to the LEVEL ﬁeld, a window appears. If you want to change the level, put the cursor on the level you want and press .
Page 179: Retic File Set Up
Latex Files CBC Files Retic Files Expected Range Auto Stop oooooooooooo oooooooooooo oooooooooooo oooooooooooo ooooooooooo CBC/Diff/Retic (932231) COPYRIGHT 1989-1993 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 08/24/93 14 58 DMS↑ PR↑ TK↑ HC↑ DB↑...
Page 180: How Cell Controls Are Processed
CONTROLS In the HOST ﬁeld, press to toggle setting to ON or OFF . If AutoTransmit is turned on (from in Main Menu or Run Other Samples), setting this HOST ﬁeld to ON enables automatic transmission of each control as it is run.
Page 181: Qc Status
Table 11 describes where results go in these circumstances, depending on the DMS display and on whether the ID read by the STKS matches an ID in a set up DMS control ﬁle. Table 11 Where Control Results Go...
Page 182: Control Error Conditions
CONTROLS CONTROL ERROR CONDITIONS If an error condition occurs on a control run, a message appears on the Error Line at the bottom of the screen. For CBC, CBC/DIFF and Retic controls, if the Auto-Stop option is ON, the system stops. If the Auto-Stop option is OFF , the system continues.
Page 183 CONTROL ERROR CONDITIONS • If the control was not identiﬁed and a Control Run ﬁle is on display, the data is lost. Example: DISK DRIVE IS FULL, CANNOT SAVE CONTROL RUN File not Found (CBC/DIFF and Retic controls only) • A control was identiﬁed but there is no control ﬁle set up.
Page 184 CONTROLS • Control run data is lost if control was identiﬁed. If control was not identiﬁed, the data is saved to the data base. • To store new control runs, delete the last run(s) or the ﬁle. Example: CONTROL FILE 11, Normal, IS FULL Control out of Range •...
Page 185 CONTROL ERROR CONDITIONS • Clear the error line and restart by pressing then START/ CONT • Or turn the Auto-Stop option off by selecting Special Functions Control Set Up AutoStop Lot and Shift Number are in Conﬂict • During control setup: A control ﬁle with the same lot number and shift already exists.
Page 186 CONTROLS Example: Error reading record 1, Control File 11. Error Writing Control File • The control ﬁle is corrupt. Example: Error reading record 1, Control File 11. Control ﬁle has no Data Available • The control ﬁle cannot print graphics because it lacks data.
Page 187 CONTROL ERROR CONDITIONS START RECEIVE SAMPLE IS IT CONTROL A CONTROL BAR CODE RUN? READ PROCESS CURRENT MATCHING DISPLAY SAMPLE SCREEN IS FILE FOUND FILE NOT CONTROL FOUND RUN? SAVE PROCESS FILE DRIVE CONTROL CONTROL IS FULL IS FULL RUN IN CONROL FILE <...
Page 188: Control Error Message Status And Action
Assign a non-conﬂicting lot # or shift. Error Reading System Stops Data Lost Power off/on. File If error recurs, call Coulter Customer Operations. Error Writing System Stops Data Discarded Power off/on. File If error recurs, call Coulter Customer Operations.
Page 189: Cbc/Diff Control File Review
CONTROL ERROR CONDITIONS Table 12 Control Error Message Status and Action Message System Status Data Status Action continued Unable to System continues No data lost Verify formatted disk in archive Drive A. Verify room on disk. No space System continues No data lost Verify room on disk.
Page 190: Cbc/Diff Control Graphics Display
Special Functions Control Run Review or Report Graphs Control Batch COULTER ≡≡≡STKS CBC/Diff/Retic (930722) COPYRIGHT 1989-1992 COULTER CORPORATION, ALL RIGHTS RESERVED F1-Help F3-Error Log F5-Other F9-Main menu F10-Previous menu 03/19/93 14:00 to scroll through results. The P DIFF LATEX CONTROL REVIEW...
Page 191 LATRON CONTROL FILE REVIEW To review the Control graphs press Set the cursor to and press GRAPHS The Mean Channel and %CV graphs appear. To review single parameter histograms of Volume, Conductivity, Scatter and Opacity from either the DIFF or RETIC LATEX CONTROL RUN screen or the DIFF or RETIC LATEX CONTROL REVIEW screen, press LATRON primer histograms are available, but are...
Page 192: Cbc Control File Review
CONTROLS CBC CONTROL FILE REVIEW Use the cursor to select the ﬁle you want, then Controls Review or Report press Select File to scroll through results. CBC CONTROL REVIEW FILE: LOT: 085300 SHIFT : IQAP ID # LEVEL: 4C Abn. High EXP DATE: 22/12/93 DATE...
Page 193: Retic Control File Review
RETIC CONTROL FILE REVIEW RETIC CONTROL FILE REVIEW Move the cursor to the ﬁle you want and press Controls Review or Report File to scroll through results. RETIC CONTROL REVIEW FILE: LOT: 539900 SHIFT : IQAP ID # 75007-1-g4-1 LEVEL: Level III EXP DATE: 12/06/93...
Page 194 CONTROLS You can transmit to Host and archive only in Control Batch Print: Date Review/Report. Type : Review/Report Host : From: Select Type Archive: No Review/Report Graphs SHIFT LEVEL LOT# SHIFT Review/Graphs NOT SETUP Level I 100000 When you select Graphs or Review/Graphs, the Diff+ Retics 107359 NOT SETUP Level I...
Page 195: Analysis
BATCH BATCH PROCESSING PROCESSING FOR FOR CONTROLS CONTROLS If you batch print or archive an entire ﬁle, the statistical data is included. If you request only part of a ﬁle (for example, you deﬁne the batch by dates that do not encompass the entire ﬁle), no statistical data is included.
Page 196: Mode To Mode
MODE TO MODE Coulter recommends that you perform quality-control checks using patient or commercial controls in both Primary and Secondary modes at intervals established by your laboratory. Investigate any failure to recover control values, within your laboratory’ s expected limits, or the presence of unexplained shifts or trends in either mode of analysis.
Page 197: Appendix A - Auto-Reporter
APPENDIX A AUTO-REPORTER 3 The COULTER AUTO-REPORTER 3 has two SINGLE-TICKET HOPPER SLOT modes: HOPPER and SINGLE. LCD DISPLAY • In the HOPPER mode, you put a stack of up SELECT to 150 tickets in the hopper and results are BUTTON printed automatically.
Page 198: Configuration
APPENDIX A CONFIGURATION To set the AUTO-REPORTER 3 conﬁguration from the DMS, Select Special Functions Set Up System Set Up Communication Deﬁnition Autoreporter Deﬁnition Set the options to Yes or No, or On or Off, by using the spacebar to toggle the setting. These options are available.
Page 199: Messages On The Auto-Reporter 3 Display
CONFIGURATION • When set to Yes, if the DMS does not have the corresponding results in its data base, the ticket remains in the printing station until the results are available. • When set to No, if the DMS does not have the corresponding results in its data base, the ticket is ejected and the next ticket feeds into the printing station.
Page 200: Messages That Print On The Auto-Reporter 3 Ticket
Ticket jammed ROM test Uxx BAD The internal memory test has Record the chip number (Uxx). found a bad location. Call Coulter Customer Operations. RAM test Uxx BAD Waiting for data The AUTO-REPORTER 3 is in the Remove the obstruction.
Page 201: D (Disabled Checksum)
MESSAGES THAT PRINT ON THE AUTO-REPORTER 3 TICKET D (disabled checksum) If the bar-code checksum is disabled in the STKS Analyzer, results printed on the AUTO-REPORTER 3 ticket have a "D" printed in the OP CODES ﬁeld. E (edited) If sample data has been edited, an "E" is printed on the ticket in the last space of the parameter edited.
Page 202: P (Positive Identiﬁer)
TAG-ERROR An internal error in the data base prevents the ticket from being printed. Try to print the ticket again. If that is not successful, call Coulter Customer Operations. ????? There was an internal error. Reprint the ticket. If that is not successful, rerun the sample.
Page 203: Auto-Reporter 3 Tests
AUTO-REPORTER 3 TESTS AUTO-REPORTER 3 TESTS There are two AUTO-REPORTER 3 tests available via the DMS Special Functions/Diagnostics routine. Before you perform either test, place a ticket without a bar-code label into either the hopper or the single-ticket slot. At the DMS, set the AUTO-REPORTER 3 conﬁguration to: •...
Page 204 APPENDIX A PN 4237188A (December 1993)
Page 205: Appendix B - Hemogard™ Closure Modification
HEMOGARD CLOSURE MODIFICATION OPERATION CHANGES There are three generations of tube processors that have been installed on new STKS Analyzers with Reticulocyte Analysis: standard glass tube with rubber stopper, HEMOGARD Closure modiﬁcation, and Universal Tube Processor. If your system has the processor designed for use with Becton Dickinson’...
Page 206 APPENDIX B When your system is modiﬁed for tubes with HEMOGARD Closure, labels on regular tubes must be placed 0.5 in. down from the cap. Figure shows how to position a bar-code label on the new tubes with HEMOGARD Closure, and on the regular tubes with a modiﬁed system.
Page 207: Appendix C - Additional Wbc Differential Qc Techniques
COMPARISON QC PROCEDURE This method for controlling the quality of the STKS differential results uses a comparison of instrument output with carefully performed stained-ﬁlm differentials. For recognition criteria for cell type reference, refer to "Reference Leukocyte Differential Count and Evaluation of Instrument Methods (H20-A),"...
Page 208 Record the mean; this is the Reference 1 value. Repeat steps 5 and 6 for the second specimen to determine the values for STKS 2 and Reference 2. Calculate the graph point for each parameter; use the following formula, and enter the result on the daily worksheet.
Page 209 COMPARISON QC PROCEDURE ® DAILY QC WORKSHEET FOR THE STKS COULTER STKS COULTER CORPORATION Miami, FL PN 4237188A (December 1993)
Page 210: Qc Graphs
0.80 When you accumulate 31 days’ worth of values, calculate your own laboratory’ s mean and 2 SD values; 95% of your graph points should fall within these limits. If they do not, call Coulter Customer Operations. PN 4237188A (December 1993)
Page 211 QC GRAPHS ® MONTHLY QC GRAPHS COULTER STKS COULTER CORPORATION Miami, FL PN 4237188A (December 1993)
Page 212: 95% Confidence Limits In Differential Counts
APPENDIX C 95% CONFIDENCE LIMITS IN DIFFERENTIAL COUNTS Table 15 deﬁnes 95% conﬁdence limits for various percentages of blood cells as determined by differential counts. Example: If 26 lymphocytes are observed in a 100-cell manual differential, the expected range of recovery is from 17 to 35 cells counted.
Page 213 95% CONFIDENCE LIMITS IN DIFFERENTIAL COUNTS Table 15 95% Conﬁdence Limits Percentages of Blood Cells as Determined By Differential Counts Observed n = 100 n = 200 n = 400 n = 800 continued 16 - 34 19 - 31 21 - 29 22 - 28 17 - 35...
Page 214 APPENDIX C Table 15 95% Conﬁdence Limits Percentages of Blood Cells as Determined By Differential Counts Observed n = 100 n = 200 n = 400 n = 800 continued 46 - 66 49 - 63 51 - 61 53 - 59 47 - 67 50 - 64 52 - 62...
Page 215 95% CONFIDENCE LIMITS IN DIFFERENTIAL COUNTS Table 15 95% Conﬁdence Limits Percentages of Blood Cells as Determined By Differential Counts Observed n = 100 n = 200 n = 400 n = 800 continued 80 - 94 82 - 92 84 - 90 85 - 89 82 - 94...
Page 216 APPENDIX C PN 4237188A (December 1993)
Page 217: Appendix D - Universal Tube Processing Module Option
UNIVERSAL TUBE PROCESSING MODULE OPTION OVERVIEW The Universal Tube Processing Module permits a STKS to run additional tube sizes and styles in the Primary Mode. This appendix lists the tubes shown to be compatible with the Universal Tube Processing Module. This is not a recommendation of the use of one tube in preference to another nor a guarantee of the acceptability of the sample tube to produce quality results.
Page 218: Becton Dickinson Vacutainer Tubes, Worldwide
APPENDIX D Note: 13-mm cassettes are also known as 5-mL cassettes. 16-mm cassettes are also known as 7-mL cassettes. BECTON DICKINSON VACUTAINER Tubes, Worldwide BD No. Volume o.d. x Length (mm) Cassette 6405 2.5 mL 13.0 x 75 13 mm 6454 7.0 mL 16.0 x 75...
Page 219: Coulter
TUBES WITHOUT ADAPTORS OR CLIPS COULTER Type o.d. x Length (mm) Cassette 5C cell control tubes 13.0 x 62 13 mm S-CAL calibrator tubes 13.0 x 62 13 mm LABO EXPRESS SERVICE (LES) LABO Type Volume o.d. x Length (mm)
Page 220: Terumo Venoject Ii Foil Top
TUBES WITH SLEEVE ADAPTORS CAUTION If your STKS does NOT have a Universal Tube Processing Module option present, do not use tube adaptors. Use of tube adaptors without the option can cause excessive partial aspirations and difficulty reading bar-code labels.
Page 221: List Of Tubes
TUBES WITH SLEEVE ADAPTORS CAUTION The clear adaptors for the COULTER MAXM, JT2 and JT3 analyzers cannot be used on the STKS. These adaptors could cause jamming of the tubes. Only use the gray sleeve adaptors with tubes processed in the Universal Tube Processing Module on the STKS.
Page 222: Labeling Requirements
APPENDIX D Labeling Requirements • You can use a maximum of two labels in addition to the sample tube manufacturer’ s label. • Place each label so that it does not cover the bottom of the tube and is ﬂat and smooth against the tube.
Page 223: Placement In Cassette
TUBES WITH SLEEVE ADAPTORS Placement in Cassette Figure shows the two types of gray sleeve adaptors used in a 13-mm cassette. You must position the gray sleeve adaptors in a cassette so that their keys (located on top of their bar-code read slits) ﬁt into the top openings of the cassette.
Page 224: Kabe
2.7 mL 11.5 x 66 Special for tubes with HEMOGARD Closure For optimal performance, Coulter recommends that you use these tubes with the Cassette Clips installed in the special cassette for tubes with HEMOGARD Closure: GREINER Vacuette Tubes GREINER o.d. x...
Page 225: Lip
TUBES WITH CASSETTE CLIPS Cassette (with Cassette Clips LIP No. Volume o.d. x Length (mm) Installed) 38887 2KE2.5/GL 2.5 mL 12.0 x 81 Special for tubes with 38908 2KE4/GL 4.0 mL 12.0 x 81 HEMOGARD Closure 38917 3KE4/GL 4.0 mL 12.0 x 81 Labeling Requirements •...
Page 226: Installing The Cassette Clips
APPENDIX D Installing the Cassette Clips Install the Cassette Clips into the special cassettes for tubes with HEMOGARD Closure. There is no need to remove them because you can use any of the tubes approved for this cassette with these Cassette Clips in place.
Page 227 HOW TO CHECK IF UNIVERSAL TUBE PROCESSING MODULE IS PRESENT Open the lower door of the Diluter. Check if the button portion of the stripper WITHOUT MODULE plate is made of black plastic. When examining your system, only evaluate the button portion of the stripper plate;...
Page 228 APPENDIX D PN 4237188B (March 1995)
Page 229: References
REFERENCES Corash L. Platelet Sizing: Techniques, Biological Significance, and Clinical Applications. Current Topics in Hematology. New York, New York: Alan R. Lise, Inc. 1983. Threatte GA, Andrados C, Ebbe S and Brecher G. Mean Platelet Volume: The Need for a Reference Method.
Page 230 REFERENCES PN 4237188B (March 1995)
Page 231: Index
INDEX Symbols .., 79, 80, 82 backwash ::::, 81, 84 message, 3 :::::, 82 bar-code labels, 54 ?????, 82 rule for Interleaved, 54 "-----", 80 sample tubes, 55 +++++, 80-82, 84 ticket, 56 -----, 80 Tubes, 60 *R, 81 bar-code wand, 165 4C cell control batch processing, 152 QC, 41...
Page 232 INDEX control review ﬁle CBC, 182 control run matrix, 170 FC under scatterplot, 82 control voltage lamp, 12 ﬂagging messages, 85 controls function ﬁnding ﬁle, 21, 43 activating, 24 CRT, 2 function keys, 5 change settings, 27, 67 Data Base Query, 139 cycling samples, 56 Data Base Query Batch, 153 Primary mode, 56...
Page 233 INDEX graphs, 181 checks at startup, 12 histograms, 181 controls and indicators, 8 light bar, 1, 14 startup, 12 line voltage meter, 8 precision of results, 79 Premix key, 5 press manual, 22 prime main unit, 1 when to, 53 manual differential, 197 whole blood, 35, 39, 42, 58, 62 menu...
Page 234 108 Analysis, 185 QC, 171 current batch, 185 status messages on Analyzer CRT, 3 graphs, 186 STKS initializing message, 3 Stop key, 5 storing specimens, 52 stripper plate release key, 5 suspect messages, 84, 85 switching between cell classiﬁcation off and on, 84...
Page 235 REVISION STATUS Issue A, 12/93 Software version 2A. Issue B, 3/95 Change pages: cover, inside front cover, iv, vi-viii, 47, 51, 85, 109, 131-136, 167, 186, 195, 207-217, 221, trademarks and back cover. Note: Changes that are part of the most recent revision are indicated in text by a black bar in the margin.
Page 237 LANGLEY FORD INSTRUMENTS, LEASE-PAK, "LFI" logo, LYSE S, MDADS, MINI- KEM, NANO-SIZER, OMNISORP, OptiChem, S-CAL, SOMACOUNT, SOMAFIX, SOMATON, STAIN RIGHT, THROMBOCOUNTER, THROMBO-FUGE, U.V.-ZYME, ZAP-OGLOBIN, ZAPONIN and ZETAFUGE are trademarks of Coulter Corporation. 3/21/95 AIM is a registered trademark of Automatic Identiﬁcation Manufacturers, Inc.
Page 238 COULTER STKS with Reticulocyte Analysis DOCUMENTATION Use and Function Reference PN 4237182 Installation (White binding) Operation Principles Speciﬁcations Precautions/Hazards Appendices References Glossary General Procedures Special Procedures and Calibration Troubleshooting PN 4237187 Cleaning Procedures (Silver binding) Replace/Adjust Procedures Troubleshooting Controls and Indicators Operator’s Guide...

COULTER STKS Operator's Manual

Controls and Indicators

Startup

Sample Analysis

Data Analysis

Shutdown

Analyzer Crt Functions

Dms Basics

Run Samples Display

Worklist

Data Base

Controls

Appendix A - Auto-Reporter

Appendix B - Hemogard™ Closure Modification

Appendix C - Additional Wbc Differential Qc Techniques

Appendix D - Universal Tube Processing Module Option

References

Index

Quick Links

Need help?

Questions and answers

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Summary of Contents for COULTER STKS