Elements eONE User Manual
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Elements eONE User Manual

Miniaturized one-channel amplifier
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eONE
Miniaturized One-Channel Amplifier
USER'S GUIDE
October 2015
elements
enabling technologies for Life Science

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Summary of Contents for Elements eONE

  • Page 1 Miniaturized One-Channel Amplifier USER’S GUIDE October 2015 elements enabling technologies for Life Science...
  • Page 2 Periodically check the following link for the most updated manual version: http://elements-ic.com/downloads/ Revision History Version Preliminary Initial Manual Created January 2014 Version 1.0 Manual Released July 2014 Version 1.1 Corrections made to figure 4 August 2014 Version 1.2 Changed manual format...

  • Page 3: Table Of Contents

    Requirements ..................................... 16 3.1.2 Installation ....................................16 3.1.3 Software Operating Procedure ..............................19 3.1.4 Graphical User Interface ................................20 3.1.5 eONE Controls ..................................... 21 3.1.6 Digital Offset Compensation ................................ 26 3.1.7 Statistic Data ....................................27 3.1.8 Trace Visualization ..................................28 3.1.9 Keiboard shortcuts ..................................

  • Page 5: Preliminary Information

    - User’s guide SECTION Preliminary Information  General notes.  Trademarks.  Instructions on disposal.  Description of safety symbols.  Qualified Personnel.  Basic knowledge required.  Proper use of the product.  Purpose of the user’s guide.

  • Page 6: Section 1

    The information in this manual is subject to change and is in no way binding upon ELEMENTS s.r.l. b) ELEMENTS s.r.l. is not responsible for technical errors or other omissions in the manual, and shall not accept any responsibility deriving from its use.

  • Page 7: Description Of Safety Symbols

    - User’s guide  Das Symbol auf dem Produkt oder seiner Verpackung weist darauf hin, dass dieses Produkt nicht als normaler Haushaltsabfall zu behandeln ist, sondern an einem Sammelpunkt für das Recycling von elektrischen und elektronischen Geräten abgegeben werden muss. Durch ihren Bei- trag zum korrekten Entsorgen dieses Produkts schützen Sie die Umwelt...

  • Page 8: Qualified Personnel

    SECTION 1 – Preliminary Information 1.5 Qualified Personnel a) eONE may be operated only by personnel qualified for the specific task in accordance with the relevant documentation for the specific task, in particular its warning notices and safety instructions. b) Qualified personnel are those who, based on their training and experi- ence, are able to identify risks and avoid potential hazards when working with these systems.

  • Page 9: Scope Of The Operating Instructions

    The operating instructions apply to the eONE family. 1.10 The manual is a part of the system b) This user’s guide belongs to eONE and is also required for commission- ing. Keep all supplied documentation for the entire service life of eONE.

  • Page 10: Safety Instructions

    1.12.1 Installation according to the instructions  Commissioning the eONE device is prohibited until it has been absolutely ensured that the system in which the eONE device is to be installed complies with all the applicable EU and international regulation. 1.13 Notes about usage ...

  • Page 11: Section 2

    - User’s guide SECTION General description and connections  Product description.  Key features.  Package.  Hardware Setup.  Front Connectors.  Rear Connectors.  Preparation for installation.  Select the location.  Checking the package contents.  Checking the operation conditions.

  • Page 12: Product Description

    SECTION 2 – General description and connections 2.1 Product description eONE (Miniaturized One-Channel Amplifier) is a new current measurement sys- tem entirely contained in a USB stick, thanks to a custom-made microchip for low-noise current signal acquisitions. Figure 1 eONE detail 2.2 Key features...

  • Page 13: Package

    - User’s guide 2.3 Package eONE package consists of: Table 2 Package eONE Software interface SMB plug adaptor USB cable Ground cable elements enabling technologies for Life Science...

  • Page 14: Hardware Setup

    SECTION 2 – General description and connections 2.4 Hardware setup 2.4.1 Front Connections eONE is designed to be as simple as possible to connect to your different set- ups. In order to obtain the best performance, the main things to be careful on are parasitic capacitances on the input and correct shielding of the overall hard- ware setup to avoid the 50/60Hz line interferences.

  • Page 15: Rear Connections

    On the rear of eONE there are two connectors: mini-B female USB connector to connect to the PC through the in- cluded USB cable; 2mm gold plated ground female connector to connect the eONE ground to the Faraday cage; elements...

  • Page 16: Select The Location

    2.4.3 Select the location The mounting location should comply with the following characteristics: a) Avoid direct sunlight exposure. b) Make sure that eONE is properly (ergonomically) accessible to the op- erator. Choose a suitable mounting height. 2.4.4 Checking the package contents ...

  • Page 17: Tipical Experimental Setup

    - User’s guide 2.4.6 Tipical Experimental Setup This picture shows how to correctly connect eONE in a typical electrophysiology setup: Figure 4 Typical Experimental Setup To obtain the best noise performances a particular care must be given to the Warning: shielding with a metallic cage (Faraday cage) all around the experimental setup.
  • Page 18 SECTION 2 – General description and connections elements enabling technologies for Life Science...

  • Page 19: Section 3

    - User’s guide SECTION Software installation and description  Software guide.  Requirements.  Installation.  Software Operation Guide.  Graphical User Interface.  eONE Controls.  Digital Offset Compensation.  Statistic Data.  Trace Visualization.  Save Data.

  • Page 20: Software Guide

    3.1 Software guide Elements Data Reader (EDR) is the control software for eONE devices. It enables the total control of eONE devices features (such as set the data acquisi- tion properties and the control voltage) and enables the real time data visualiza- tion and data saving.
  • Page 21 - User’s guide  During the installation process you will be asked to install the FTDI driver needed to communicate through USB to the eONE devices. Figure 7 FTDIChip CDM Drivers  Click on Extract to prepare the installation of these drivers.
  • Page 22 After the software installation has ended, connect the eONE device to a free USB port.  The first time you connect the eONE to your PC, you must wait for the new USB device complete recognition. elements enabling technologies for Life Science...

  • Page 23: Software Operating Procedure

    The configuration file is loaded by clicking on the “Open Config” button.  The configuration file is eONE-B-Vxx.ccf or eONE-VP-Vxx.ccf or eONE- HS-Vxx.ccf (depending on the eONE version) and can be found in the EDR installation folder (usually C:\Program Files (x86)\EDR 2). ...

  • Page 24: Graphical User Interface

    (see the “Statistic Data” section for details) and, on the right there are the con- trols to associate an oscilloscope-like trace (beam) with a specific input signal in the Display window (in the case of eONE devices, there is only one input channel available). See the “Trace Visualization” section for details.

  • Page 25: Eone Controls

    - User’s guide 3.1.5 eONE Controls The eONE Controls window contains controls in a modular fashion with some fea- tures enabled or disabled depending on the eONE version. Below is described the HS version control tab which include all the possible features.
  • Page 26 The Digital compensation check box enables or disables the offset compensation function (see the “Digital Offset Compensation” section for details). The Reset check box keep the eONE device in reset mode. This function can be useful to reset the digital offset compensation value to the initial value.
  • Page 27 Constant voltage protocol: The constant voltage is considered as a protocol with only the Vhold parame- ter. Figure 14 eONE Controls – Voltage Protocols Constant Voltage protocol  Triangular wave protocol: It can be used to control the bilayer formation and it is used for the Capaci- tance estimation feature (see details section 3.1.7).
  • Page 28 Vfp mV. The time interval between two consecutive pulses (interpulse interval) changes of the value of the Tstep parameter. It can be positive or negative to obtain increasing or decreasing interpulse intervals. Figure 18 eONE Controls – Voltage Protocols Recovery from inactivation protocol  Variable duration pulses protocol: It consist of a series of rectangular voltage pulses (Vfp mV of fixed amplitude) of a duration that changes of the value of the Tstep parameter.
  • Page 29 - User’s guide Finally, below are listed the controls that are present only in the eONE Base ver- sion, which has some limitations compared to the VP and HS versions:  Triangular Wave check box  Vhold tab The Triangular Wave control is used to force a 50 Hz triangular wave control volt- age of peak to peak amplitude of 200 mVpp.

  • Page 30: Digital Offset Compensation

    3.1.6 Digital Offset Compensation To compensate the voltage offset due to internal opamp offsets or unwanted electrode potentials, eONE has an internal calibration procedure that can be acti- vated with the Digital Compensation check box. The compensation circuit, when activated, sets the Vc to 0 V and adds to it a vari- able voltage in the range from -128 mV to +128 mV until the current is 0 A (1 mV step resolution).

  • Page 31: Statistic Data

    The following instructions have to be followed to obtain capacitance and re- sistance estimations. Capacitance estimation:  Enable the Triangular Wave using the check box (eONE Base version) or apply the Protocol 1 (eONE VP or HS version); Note: ...

  • Page 32: Trace Visualization

    Pauses the data display devices continues to acquire and possibly record data Changes the vertical offset (shift) Search for input current signal and set it in the middle of the window elements enabling technologies for Life Science...

  • Page 33: Keiboard Shortcuts

    - User’s guide NB: it is possible to Activates the trigger func- select the positive or tion using the selected negative edge of the value as threshold Vc or of the input cur- rent signals Displays the two data sig-...
  • Page 34 (usually computer when performing long-time C:\\Program Files (x86)\Elements Data Reader) and in the folder where data are experiments. saved. Data samples are stored in 32 bit single precision format, little endian.
  • Page 35 - User’s guide % check if the header file is present headerFile = dir(strcat(pathname, basefilename, '*.txt')); isempty(headerFile) headerFile = dir(strcat(pathname, basefilename, '*.edh')); length(headerFile) == 1 fid = fopen(fullfile(pathname, headerFile.name)); % open header file for read % look for range and bandwidth data while line = fgetl(fid);...
  • Page 36 OSR = 512; case OSR = 128; case OSR = 64; case OSR = 32; otherwise display('Error: invalid selection'); return % select range display('Select range:'); display('1) 200 pA'); display('2) 20 nA'); sel = input('select 1,2: elements enabling technologies for Life Science...
  • Page 37 - User’s guide switch case range = 0; case range = 1; otherwise display('Error: invalid selection'); return % read files B = []; i = 1:N fid = fopen(fullfile(pathname, files(i).name)); % open file for read A = single(fread(fid, 'single'));...
  • Page 38 Save data in .abf format selecting it in the “FilePreferences” menu of EDR software;  Open Clampfit® and click on “Open Data” (or Ctrl+O shortcut) and select the saved Axon Binary File to read the two acquired data channels: cur- rent signal and control voltage; elements enabling technologies for Life Science...

  • Page 39: Troubleshooting Guide

    Wrong connections Check input- and RMS noise values or use of too long back-connections higher than expected connection wires or (see section 2.4.6) coaxial cables elements enabling technologies for Life Science...

  • Page 40: Technical Support & Repairs

    Technical support & repairs Technical support & repairs ELEMENTS S.R.L. offers wide-ranging, complete after-sales technical support. The staff who deal with this handle questions on the entire range of products skillfully, quickly, and efficiently. You can phone our staff in the service department, and they will give you com- plete, prompt advice on how to resolve your problems.
  • Page 41 Figure 11 Elements Data Reader Complete GUI ....................... 20 Figure 12 eONE Controls - HS version ..........................21 Figure 13 eONE Controls – Voltage Protocols Rectangular Voltage Pulses protocol ............22 Figure 14 eONE Controls – Voltage Protocols Constant Voltage protocol ............... 23 Figure 15 eONE Controls –...
  • Page 42 Life Science Elements s.r.l. via Montalti, 42 47521 Cesena ITALY email: info@elements-ic.com web: www.elements-ic.com...

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