Venous Whole Blood Samples; Interfering Substances; Equipment/Materials/Reagents - CardioChek PLUS User Manual

5. Gently apply pressure to the finger near the first knuckle of the ring or middle finger and
engorge the end of the finger with a squeeze and release technique.
6. Use the first drop of blood to immediately dose the tip of the eGLU test strip until the
channel is filled and the screen shows a beating heart icon over the glucose strip.
7. Wipe away the residual blood with a clean, dry gauze pad and use the second blood
drop to begin the capillary tube collection of 40µL of blood to dose the Lipid Panel test
strip. With a gentle, but firm squeeze near the first knuckle, produce a large drop at the
fingerstick site and touch the tip of the capillary blood collector or calibrated pipette to
the drop. With 2 to 3 squeeze-and-release motions, allow the horizontal fill of the
capillary tube without the introduction of bubbles or air spaces.

In order to prevent air bubbles or gaps, hold the capillary tube at a slight upward
angle and fill the capillary tube to the black line.
8. See section VI to perform the test.
B. Venous Whole Blood Samples
1. Collect the venous sample in either a green top (Lithium heparin) or lavender top (EDTA)
tube.
2. Gently mix the tube by inversion 10 times.
3. Carefully open the tube and remove a 40µL sample using either a capillary blood
collector or calibrated pipette.
4. See section VI to perform the test.
5. Venous samples are not suitable for eGLU testing and are only allowed for PTS
reflectance test strips, such as the Lipid Panel test portion of the Smart Bundle pack.
C. Interfering Substances
Dopamine and methyldopa decreased the results of all lipid analytes, while causing higher
results for the eGLU testing. Extremely high doses of ascorbic acid (Vitamin C) decreased the
results of all the lipid analytes, while having no significant effect on the eGLU results.
IV.

Equipment/Materials/Reagents

The Lipid + eGLU Smart Bundle™ consists of three boxes of Lipid Panel test strips which are self-
contained disposable test strips preloaded with the reagents necessary to measure total
cholesterol, HDL cholesterol, and triglycerides, as well as one box of eGLU™ test strips preloaded
with the reagents necessary to measure glucose. Calculated LDL results are available for
samples with total cholesterol and HDL cholesterol within the measuring range of the analyzer,
and triglycerides levels below 400 mg/dL.
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