Leaf-Clip Holder 2030-B - Walz MINI-PAM Manual

CHAPTER 8
light may show an actinic effect. This can be counteracted by use of
the ML-BURST function (MODE-menu position 5, see 12.3.2).
Because of the strong influence of sample distance on signal
amplitude, particularly with the 2 mm Ø fiberoptics, it is
recommended to clamp the fiberoptics tip at fixed distance to the
sample surface. Otherwise there may be substantial errors, even in
the ratio measurement of ∆F/Fm, when the distance changes between
the consecutive measurements of F and Fm, separated by
approximately 1 s (see 12.3.7).
8.3

Leaf-Clip Holder 2030-B

The Leaf-Clip Holder 2030-B may substitute for the standard
'Distance Clip' as a device for defined positioning of the fiberoptics
relative to the leaf plane. The leaf is resting on a perspex tube with
widened crest, which can be vertically adjusted, to account for
different leaf thicknesses. The fiberoptics axis forms a 60° angle with
the leaf plane. In this way shading of the sample can be largely
avoided when external actinic illumination is applied. For special
applications using the internal actinic lamp a 90° fiberoptics adapter
(2030-B90) is available which can be readily mounted instead of the
standard 60° adapter. This is e.g. particular useful for recordings of
LIGHT CURVES, providing more homogenous illumination and
higher signal amplitudes (see 12.3.7). The distance between
fiberoptics and leaf can be varied. Standard distances are defined by
spacer rings. In addition, the Leaf-Clip Holder 2030-B displays the
following features:
COMPONENTS OF THE MINI-PAM
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