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Summary of Contents for Seahorse XFp
- Page 1 XFp Extracellular Flux Analyzer User Manual Part #102745-400 Rev A...
- Page 2 102745-400 Rev A December 2014 Revision history: First release December 2014 Copyright: Copyright ©2014 Seahorse Bioscience Inc. All rights reserved. Seahorse Bioscience Inc. 16 Esquire Road N. Billerica, MA 01862 Submit technical support questions or comments about our documentation to: support@seahorsebio.com...
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Page 3: Table Of Contents
Running an XFp Assay ......................... 11 Modifying XFp Analyzer Settings ....................14 XFp Analyzer Diagnostics ......................16 Online Help and Support ......................18 Analyzing XFp Data Using Wave for Desktop ................19 Appendices Appendix I: Networking Guide ....................20 Appendix II: Troubleshooting ..................... 24... -
Page 4: Xfp Analyzer Overview
_____________________________________________________________________________________________________________________ 1. XFp Analyzer Overview The Seahorse Bioscience XFp Analyzer measures the rate of change of dissolved oxygen and pH in the media immediately surrounding living cells cultured in a miniplate. Changes in the extracellular media are caused by the consumption or production of analytes by the cells. Therefore, a sensitive measurement of the media flux can be used to determine rates of cellular metabolism with precision and in a completely non-invasive, label-free manner. -
Page 5: Technical Specifications
XFp Extracellular Flux Analyzer User Manual _____________________________________________________________________________________________________________________ 2. Technical Specifications Model Dimensions: Width x height x depth 12” x 17” x 23” 30 cm x 43 cm x 58 cm Weight: 33 lbs / 15 Kg Power Requirements 100-240V AC 50/60Hz... -
Page 6: Xfp Analyzer Installation
Unpacking and Component Identification Upon receipt immediately check each box for damage. Report any shipping damage to the transportation company, and Seahorse Bioscience using the contact information on page 2 of this document. The following items are included in an XFp system:... -
Page 7: Suitable Locations For The Xfp Analyzer
WARNING: The protection provided by this instrument may be compromised if it is used in a manner not specified by Seahorse Bioscience. Safe operation of the XFp Analyzer requires that all covers be securely attached and plate tray door is closed. This also prevents heat loss and system cooling, which can affect data quality. -
Page 8: Basic Operation Of The Xfp Analyzer
To turn off the instrument, press the power button in the lower-left corner of the Home screen to first shut down the XFp Analyzer screen. After the screen turns black, use the power switch on the back of the instrument to completely shut down power. -
Page 9: Navigating The Xfp Analyzer Software
_____________________________________________________________________________________________________________________ 5. Navigating the XFp Analyzer Setting up an XFp Assay To set up an assay on the XFp Analyzer, select Start from the Home screen. 1. Select Template from Local, USB or Network drive Seahorse Bioscience provides default templates for XF Stress Test and cell characterization assays. - Page 10 Click Start Assay then follow the on-screen instructions. NOTE: The XFp Analyzer will save assay results to a USB flash drive, if available. Seahorse Bioscience recommends inserting a USB flash drive into the instrument at the time of assay...
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Page 11: Running An Xfp Assay
Wait for the user prompt to appear. 3. Following calibration, the XFp Analyzer tray will open and present the utility plate. Remove the Utility Plate and load the Cell Plate. Ensure the lid is removed from the Cell Plate prior to loading onto the XFp Analyzer tray. - Page 12 _____________________________________________________________________________________________________________________ 5. During the assay, two views are automatically available to visualize the data being acquired by the XFp Analyzer. Select either tab to toggle between the views. a. Overview The overview tab displays both OCR and ECAR as a function of time.
- Page 13 Assay Result File (*.asyr). Note: Canceling a run during an assay may cause the XFp Analyzer to stall. If this occurs, restart the hardware by using the power switch on the back of the instrument. After shutting down, use...
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Page 14: Modifying Xfp Analyzer Settings
XFp Extracellular Flux Analyzer User Manual _____________________________________________________________________________________________________________________ Modifying XFp Analyzer Settings From the Start page, touch the Settings button to access the XFp Analyzer Settings page containing: • System Preferences o Environmental Settings o Backup Assay Results o Auto Assay Naming o Template Management •... - Page 15 1) Check the box next to an individual assay template or click Select All to select all the assay template files. 2) Click Import (from either the USB or Network tab) to import files onto the XFp Analyzer. -OR- b. Click Export (from XFp Analyzer Local storage) to export files to a USB or network location.
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Page 16: Xfp Analyzer Diagnostics
XFp Extracellular Flux Analyzer User Manual _____________________________________________________________________________________________________________________ XFp Analyzer Diagnostics The XFp Diagnostics page contains a variety of functions that assist Seahorse Technical Support in debugging any issues or errors encountered during normal operation. The System Check diagnostic function should be performed upon initial startup of the XFp Analyzer and upon Seahorse Technical Support request. - Page 17 Cartridge Eject – Use this function to Eject or Load a cartridge into the XFp Analyzer*. *Note: If a cartridge is suspected to be inside the XFp Analyzer, use this function to remove the cartridge before beginning another assay. Air filter replacement: At approximately one-year intervals, the air filter at the upper rear of the analyzer should be replaced.
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Page 18: Online Help And Support
Software Updates (Product Update Available) or Firmware Updates (Firmware Update Available) will be clickable. 3. “Help” Text Info - Most screens on the XFp Analyzer have a small Help button that will display Help text related to the current screen. -
Page 19: Analyzing Xfp Data Using Wave For Desktop
_____________________________________________________________________________________________________________________ Analyzing XFp Data using Wave for Desktop While assay design can either be done on the XFp Analyzer or in Wave for Desktop, Seahorse recommends Assay Result files (*.asyr) be analyzed using Wave for Desktop. Note that Wave for Desktop can be downloaded and installed on any personal computer via the Seahorse Bioscience website. -
Page 20: Appendix I: Networking Guide
The two options for network connection are (1) wired via Ethernet cable or (2) wireless via Wireless USB Adapter. Wired Connection 1. Plug the wired network connection into the back of the XFp Analyzer. Ensure that the connection is firmly seated. 2. Power the XFp Analyzer OFF 3. - Page 21 XFp Extracellular Flux Analyzer User Manual _____________________________________________________________________________________________________________________ 3a. Wired Setup 1. From the Home screen select the Settings button. 2. Select the Go to Setup button. The Wired Network Connection Settings screen will appear. There are two options to use, depending on the settings required by the facility IT...
- Page 22 XFp Extracellular Flux Analyzer User Manual _____________________________________________________________________________________________________________________ 3b. Wireless Setup 1. From the Home screen, select the Settings button 2. Click the Go to Setup button 3. Click the right arrow (bottom right) once. The Wired Network Connection Settings screen will appear.
- Page 23 XFp Extracellular Flux Analyzer User Manual _____________________________________________________________________________________________________________________ 5. Email Setup 1. From the Home screen, click Settings and then click Go To Setup 2. Click the right arrow at the bottom three times. The Email Configuration tab will appear. 3. Enter the required information and enter a working test e-mail address into the Test Email Address dialogue box.
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Page 24: Appendix Ii: Troubleshooting
• The XFp Analyzer will eject a cartridge if one already been loaded is left inside the instrument. For any other problems or questions, please contact Seahorse Technical Support at support@seahorsebio.com Refer to section 5 of this document for a detailed description of running instrument diagnostics to aid in troubleshooting problems. -
Page 25: Appendix Iii: Support Contact And Ordering Information
Global/ United States: +1 (978) 671-1600, option # 3 United Kingdom: 0800 096 7632 Germany: 0800 180 6678 Europe: +45 31 36 98 78 Consumables Order To order XFp FluxPaks (including cell culture plates, sensor cartridges and calibrant solution) or other supplies: United States: Email: orders@seahorsebio.com...
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