Beckman Coulter SW 41 Ti User Manual
  1. Manuals
  2. Brands
  3. Beckman Coulter Manuals
  4. Engine
  5. SW 41 Ti
  6. User manual

Beckman Coulter SW 41 Ti User Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

L5-TB-047Q
November 2007
SW 41 Ti Rotor
Used In Beckman Coulter
Class H, R, and S
Preparative Ultracentrifuges
© 2007 Beckman Coulter, Inc.
Printed in the U.S.A.

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the SW 41 Ti and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Summary of Contents for Beckman Coulter SW 41 Ti

  • Page 1 L5-TB-047Q November 2007 SW 41 Ti Rotor Used In Beckman Coulter Class H, R, and S Preparative Ultracentrifuges © 2007 Beckman Coulter, Inc. Printed in the U.S.A.

  • Page 2: Safety Notice

    Although rotor components and accessories made by other manufacturers may fit in the SW 41 Ti rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the SW 41 Ti rotor may void the rotor warranty and should be prohibited by your laboratory safety officer.

  • Page 3: Specifications

    SW 41 Ti Rotor U.S. Pat. No. 3,393,864; Japanese Pat. No. 739,613; British Pat. No. 1,145,005; Axis of Rotation German Pat. No. 1,598,174. SPECIFICATIONS Maximum speed ........41 000 rpm Density rating at maximum speed.
  • Page 4 ISO 9001 or 13485 facility for use with the appropriately classified Beckman Coulter ultracentrifuge. The SW 41 Ti, rated for 41 000 rpm, is a swinging bucket rotor designed to centrifuge up to six tubes. Used in Beckman Coulter class H, R, and S preparative ultracentrifuges, the rotor develops centrifugal forces for the separation and purification of small particles.

  • Page 5: Preparation And Use

    SW 41 Ti Rotor PREPARATION AND USE Specific information about the SW 41 Ti rotor is given here. Informa- tion common to this and other rotors is contained in Rotors and Tubes for Preparative Ultracentrifuges (publication LR-IM), which should be used together with this manual for complete rotor and accessory operation.
  • Page 6 SW 41 Ti Rotor ROTOR PREPARATION For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration. Hanger 1. Load the filled containers into the buckets (see page 8 for tube and (331763) accessory information). Complete loading by placing the correct Gasket floating spacers (if required) over the tubes.
  • Page 7 (clockwise) by hand to check for proper installation. ➠ NOTE The aluminum handle supplied with the SW 41 Ti rotor is not interchangeable with similar handles supplied with other rotors. 2. Refer to the instrument instruction manual for ultracentrifuge operation.
  • Page 8 TUBES AND ACCESSORIES The SW 41 Ti rotor uses tubes and accessories listed in Table 1. Be sure to use only those items listed, and to observe the maximum speed limits shown. Refer to Appendix A in Rotors and Tubes for information on the chemical resistances of tube and accessory materials.
  • Page 9 SW 41 Ti Rotor Table 1. Beckman Coulter Tubes for the SW 41 Ti Rotor. Use only the items listed here. Tube Required Accessory Max Speed/ Dimensions Part Part RCF/ and Volume Description Number Description Number k Factor 14 x 89 mm...
  • Page 10 The k factor of the rotor is a measure of the rotor’s pelleting TIME HR:MIN efficiency. (Beckman Coulter has calculated the k factors for all of its preparative rotors at maximum rated speed and using full tubes.) The k factor is calculated from the formula: ⁄...
  • Page 11 SW 41 Ti Rotor Use the k factor in the following equation to estimate the run time t (in hours) required to pellet particles of known sedimentation coeffi- cient s (in Svedberg units, S). Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor as follows: ⎛...
  • Page 12 10 000 17 200 12 300 7 550 2078 *Calculated for all Beckman Coulter preparative rotors as a measure of the rotor’s relative pelleting efficiency, in water at 20°C. Relative Centrifugal Fields SW 41 Ti Rotor 300 000 250 000...
  • Page 13 The reference curves in Figure 3 show gradient distribution at equi- librium. Each curve in Figure 3 is within the density limits allowed for the SW 41 Ti rotor: each curve was generated for a single run speed using the maximum allowable homogeneous CsCl densities (one for each fill level) that avoid precipitation at that speed.
  • Page 14 SW 41 Ti Rotor 1.90 Figure 2. Precipitation Curves full for the SW 41 Ti Rotor. Using combinations of rotor speeds and homogeneous CsCl solution densities that intersect on or below these curves ensures 1.80 that CsCl will not precipitate during centrifugation.
  • Page 15 1 /2-filled 1 /4-filled Distance from Axis of Rotation (mm) Figure 3. CsCl Gradients at Equilibrium for the SW 41 Ti Rotor. Centrifugation of homogeneous CsCl solutions at the maximum allowable speeds (from Figure 2) results in gradients presented here.
  • Page 16 SW 41 Ti Rotor or from homogeneous solutions. But the total amount of CsCl in solu- tion must be equivalent to a homogeneous solution corresponding to the concentrations specified in Figure 3.) Figure 3 can also be used to approximate the banding positions of sample particles. Curves not shown in the figure may be interpolated.
  • Page 17 SW 41 Ti Rotor 4. Particles will band at these locations across the tube diameter at At Speed equilibrium during centrifugation. Floating Pelleted Components Bands Material In this example, particles will band about 138 and 141.5 mm from the axis of rotation, about 3.5 mm of centerband-to-centerband separation.

  • Page 18: Care And Maintenance

    Spinkote lubricant (306812) before every run. Refer to Appendix A in Rotors and Tubes for the chemical resistances of rotor and accessory materials. Your Beckman Coulter representa- tive provides contact with the Field Rotor Inspection Program and the rotor repair center.
  • Page 19 SW 41 Ti Rotor ➠ NOTE Do not immerse the rotor body in water, since the hanger mechanism is difficult to dry and can rust. 3. Rinse the cleaned rotor and components with distilled water. 4. Air-dry the buckets upside down. Do not use acetone to dry the rotor.
  • Page 20 SW 41 Ti Rotor While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfec- tion is a concern, consult your laboratory safety officer regarding proper methods to use.
  • Page 21 Before returning a rotor or accessory for any reason, prior permission (a Returned Goods Authorization form) must be obtained from Beckman Coulter, Inc. This RGA form may be obtained from your local Beckman Coulter sales office, and should contain the following information: •...

  • Page 22: Supply List

    REPLACEMENT ROTOR PARTS SW 41 Ti rotor assembly........331362 Buckets (set of 6, with caps and gaskets) .
  • Page 23 Preparative Ultracentrifuge Rotors ..5 years — No Proration 2) If a Beckman Coulter centrifuge is damaged due to a failure of Analytical Ultracentrifuge Rotors..5 years — No Proration...
  • Page 24 Telephone: (27) 11-805-2014/5 Fax: (27) 11-805-4120 e-mail: beckman@intekom.co.za Beckman Coulter, Inc. • 4300 N. Harbor Boulevard, Box 3100 • Fullerton, California 92834-3100 Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com • Telex: 678413 • Fax: 1-800-643-4366 ©2007 Beckman Coulter, Inc. Printed on recycled paper...

Table of Contents

Save PDF