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HARVARD PHD 4400 Hpsi Programmable User Manual

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PHD 4400
Syringe Pump Series
User's Manual
PHD 4400 Hpsi Programmable
MA1 70-2200
PHD 4400 Hpsi Remote Programmable
MA1 70-2201
Publication 5406-001-REV-D

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Summary of Contents for HARVARD PHD 4400 Hpsi Programmable

  • Page 1 PHD 4400 Syringe Pump Series User's Manual PHD 4400 Hpsi Programmable MA1 70-2200 PHD 4400 Hpsi Remote Programmable MA1 70-2201 Publication 5406-001-REV-D...
  • Page 2 WEEE/RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers: We are committed to being a good corporate citizen. As part of that commitment, we strive to maintain an environmentally conscious manufacturing operation. The European Union (EU) has enacted two Directives, the first on product recycling (Waste Electrical and Electronic Equipment, WEEE) and the second limiting the use of certain substances (Restriction on the use of Hazardous Substances, RoHS).

  • Page 3: Table Of Contents

    Table of Contents SUBJECT PAGE NO. Manual Description ..........4 General Information: Warranty ..............4 Repairs ..............4 Serial Numbers ............4 Calibrations ............4 General Safety Summary ........5 Technical Specifications..........6 Theory of Operation ..........7 Pressure and Speed ..........8 Features: Built-in Syringe Table and Custom Syringes ..8 Infusion and Refill Rates ........8 Target Volume ............8 Auto Fill ..............8...
  • Page 4 Table of Contents SUBJECT PAGE NO. Syringe Loading ..........15 Operation: Running the Pump ..........16 Diameter ............16 Infuse Rate ............16 Refill Rate ............16 Target Volume ............17 Auto Fill ..............17 Selecting the Run Mode ........17 Program Description ..........19 Program Mode: Entering a Program..........19 Sequence Operation ..........20 Program Printout ..........23 Program Run Time Error Messages....23...
  • Page 5 Table of Contents SUBJECT PAGE NO. Table of Syringe Diameters ......45 Appendices: Stainless Steel Syringe ......46 Nominal Min/Max Flow Rates ....48 Custom Applications ........48 Pressure and Force Specifications ....49 PHD 4400 Hpsi to PC Connection ....49 RS-232C Specifications......50 Symphony ..........50 User I/O Connector Specifications ....51 Accessories and Spare Parts ....51 Maintenance and Troubleshooting ....52...

  • Page 6: General Information: Manual Description

    Harvard Apparatus warranties this instrument for a period of two years from date of pur- chase. At its option, Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or materials. This warranty does not extend to damage result- ing from misuse, neglect or abuse, normal wear and tear, or accident.

  • Page 7: General Safety Summary

    General Safety Summary Please read the following safety precautions to ensure proper use of your syringe pump. To avoid potential hazards and product damage, use this product only as instructed in this manual. To Prevent Hazard or Injury: U U s s e e P P r r o o p p e e r r L L i i n n e e C C o o r r d d Use only the specified line cord for this product and make sure line cord is certified for country of use.

  • Page 8: Technical Specifications

    Specifications Specifications Accuracy ±0.35% Reproducibility ±0.05% Syringes (Min. / Max.) 0.5 µ l/140 ml Syringe Diameter 50 mm Flow Rate: Minimum 0.0001 µ l/hr (with 0.5 µ l syringe) Maximum 220.82 ml/min (with 140 ml syringe) 374.39 ml/min (with 50 mm diameter syringe) Calibration Automatic Display...

  • Page 9: Theory Of Operation

    The numerous features of the PHD 4400 Hpsi result from the use of microprocessor technology. The PHD 4400 Hpsi Programmable model provides full programmability along with Infuse/Withdraw capability. Use of the REFILL RATE key, INFUSE/REFILL key, PRO- GRAM key, AUTO FILL key, Retaining Brackets and Adjustable Stops are all described in detail in Section 8 of this manual.

  • Page 10: Features

    Features P P r r e e s s s s u u r r e e a a n n d d S S p p e e e e d d PHD 4400 Hpsi can deliver up to 220.82 ml/minute with a single 140 ml syringe. Maximum pressure is dependent on syringe size.

  • Page 11: External Connections

    Features E E x x t t e e r r n n a a l l C C o o n n n n e e c c t t i i o o n n s s User I/O Allows pump operations to be synchronized with external devices or by a person at a distance from the pump.

  • Page 12: User Interface

    User Interface The user interface consists of a keypad with a display area The display consists of a 2 line, 40 character alphanumeric vacuum fluorescent display. The display will show one of three types of messages: Default display, setting display, or an informational message. The top line of the display is divided into three sections.

  • Page 13: Description Of Keys

    User Interface: Description of Keys P P H H D D 4 4 4 4 0 0 0 0 Figure 1. Programmable Model Keypad S S e e t t K K e e y y s s SET – Allows modification of a data item in this group of keys. To modify a data item, press the relevant key after pressing the SET key.

  • Page 14: Entering Data

    User Interface: Entering Data S S e e t t K K e e y y s s Keys with rectangular outlines in this group are used to modify or review settings of the pumps control data. To review the current setting of a control data item, simply hold down the relevant key and the data setting will appear in the top line of the dis- play.
  • Page 15 User Interface: Entering Data Step Setting RS-232C Protocol Description SET WHAT? Press and release SET key Pump Mode MODEL 44 PROTOCOL Press and release 1 key Pump Mode Current RS-232C protocol displayed MODEL 22 PROTOCOL Press and release 1 key to Pump Mode change RS-232C protocol XXXXX ml INFUSE...

  • Page 16: Initial Setup

    Initial Setup Figure 2a. Rear Panel: Power Entry and Serial Number Label Read the entire manual to become familiar with all features and functions of the PHD 4400 Hpsi. The operating voltage range for the PHD 4400 Hpsi is 100 - 240 VAC, 50/60 Hz. Use only the specified line cord for this product and make sure line cord is cer- tified for country of use.

  • Page 17: Diameter

    Operation Figure 3. Fuse Replacement Because of the wide range of functions that the PHD 4400 Hpsi is capable of per- forming, certain information about your application must be entered into the pump. At minimum, the pump needs to know the diameter of your syringe, the infusion rate and direction of travel.
  • Page 18 If desired, adjustable stops to limit the syringe plunger travel may be purchased from Harvard Apparatus (see Appendix J for part number). Install the stops on the two guide rods at the desired locations.
  • Page 19 Operation R R u u n n n n i i n n g g t t h h e e P P u u m m p p Pressing the RUN/STOP key starts the pump. The pump will operate according to the rel- evant data entered as interpreted by the selected run mode.

  • Page 20: Target Volume

    Operation T T a a r r g g e e t t V V o o l l u u m m e e The Target Volume is the volume that you desire to deliver at the set Infuse or Refill Rate. The pump must be set for this Volume mode for this feature to work.

  • Page 21: Entering A Program

    Program Mode P P r r o o g g r r a a m m D D e e s s c c r r i i p p t t i i o o n n A program is made up of a set of sequences. Each sequence being a set of operating instructions for the pump to follow.

  • Page 22: Sequence Operation

    Program Mode S S e e q q u u e e n n c c e e o o f f O O p p e e r r a a t t i i o o n n Use the PROGRAM key to select the sequence’s operation.
  • Page 23 Program Mode Profile Runs at specified flow rate until target volume is pumped or a time interval has elapsed. Travel direction is as specified. Data Specified: Strategy 1: Flow rate (Volume) Time Interval = 00:00:00 Target volume Pumping direction Strategy 2: Flow rate (Time) Time interval...
  • Page 24 Program Mode Dispense Repeatedly dispense specified volume. Runs at specified flow rate until a volume is pumped or a time interval has elapsed, then pump will stop. If no time interval was specified (Strategy 1), the display will show ‘TRIGGER’ and the next dispense will begin after an external or keyboard run command.
  • Page 25 Program Mode TTL Out Programmable TTL Pin. TTL output Pin 4 of the user I/O connector can be set to a HIGH or LOW level from within a program. TTL pin level Data Specified: Restart Immediately restart program from the first sequence. None Data Specified: Stop...

  • Page 26: User I/O Devices

    External Control Interfaces R R S S - - 2 2 3 3 2 2 C C D D e e v v i i c c e e s s Remote Unit Connector (MA1-70-21xx, MA1-71-21xx and MA1-70-2023 only) Figure 5. Rear Panel External Connections External devices that can be attached to the PHD 4400 Hpsi are categorized into either RS-232C devices or user I/O devices.

  • Page 27: Use Of Events

    External Control Interfaces Scale Use the RS-232C key to toggle between the supported manufacturers: Mettler, Sartorius and Ohaus. When a scale is attached, the weight will be read from the scale and used as the delivered volume whenever the pumping direction of the pump is set to infuse.
  • Page 28 Programming Tutorial M M u u l l t t i i p p l l e e I I n n f f u u s s i i o o n n E E x x a a m m p p l l e e SEQUENCE The following program will instruct the pump to infuse according to the above...
  • Page 29 Programming Tutorial R R a a m m p p i i n n g g U U p p I I n n f f u u s s i i o o n n R R a a t t e e E E x x a a m m p p l l e e P P R R O O G G R R A A M M P P R R I I N N T T O O U U T T SEQUENCE PROG1 SEQ 1: PROFILE...
  • Page 30 Programming Tutorial M M u u l l t t i i p p l l e e D D i i s s p p e e n n s s i i n n g g E E x x a a m m p p l l e e P P R R O O G G R R A A M M P P R R I I N N T T O O U U T T PROG1 SEQ 1: DISPENSE SEQUENCE...
  • Page 31 Programming Tutorial P P e e r r i i o o d d i i c c D D i i s s p p e e n n s s e e L L o o o o p p E E x x a a m m p p l l e e 43:30 5:00 5:00...
  • Page 32 Programming Tutorial C C o o m m b b i i n n a a t t i i o o n n o o f f I I n n f f u u s s i i o o n n a a n n d d W W i i t t h h d d r r a a w w P P r r o o f f i i l l e e s s E E x x a a m m p p l l e e Here is an example of a more com- SEQUENCE...
  • Page 33 4 is set to a logic high for the duration of the bolus after which it is dropped. This output pin can be attached to the timer input of another pump, such as a Harvard Pump 22 or 44, to create a precise mixture during the bolus. After the bolus i i s s c c o o m m - - p p l l e e t t e e d d , , the Pump 22 would be stopped and the PHD 4400 Hpsi would return to deliv- ering its background rate of 300 ml/hr, waiting for another external event.
  • Page 34 Programming Tutorial Figure 12. Use of TTL Signal Before the pumping flow begins, the pump sends a TTL signal to another piece of equip- ment for 5 seconds, but first verifies that the TTL line is low by turning it off for 1 second, then turns it on.
  • Page 35 The PHD 4400 Hpsi is capable of using two sets of pump interface commands. This will allow users of Harvard Apparatus Model 22 and Model 44 pumps to use existing programs to control the PHD 4400 Hpsi pumps. The two sets of commands are con- tained on the following pages.
  • Page 36 Pump Chain Commands Each command sent to the pump chain is a string of ASCII characters, with leading zero’s on numbers and all spaces optional. Numbers are a maximum of five digits. The following symbols are used in describing the commands: P P u u m m p p C C h h a a i i n n C C o o m m m m a a n n d d s s - - M M o o d d e e l l ‘...
  • Page 37 Pump Chain Commands Q Q u u e e r r i i e e s s - - R R e e s s p p o o n n s s e e : : C C R R L L F F V V a a l l u u e e C C R R L L F F P P r r o o m m p p t t Q Q u u e e r r i i e e s s w w i i t t h h N N u u m m e e r r i i c c R R e e s s p p o o n n s s e e Send diameter value, units in mm Send rate value in current range units...
  • Page 38 Pump Chain Commands P P u u m m p p C C h h a a i i n n C C o o m m m m a a n n d d s s - - M M o o d d e e l l ‘ ‘ 4 4 4 4 ’ ’ P P r r o o t t o o c c o o l l Symbol Meaning [.
  • Page 39 Pump Chain Commands P P u u m m p p C C o o m m m m a a n n d d s s a a n n d d R R e e s s p p o o n n s s e e s s Starts pumping according to the present setting of the pump.
  • Page 40 Pump Chain Commands RFR [<float> [<units>]] Request to set or query refill rate setting. Set refill rate: RFR rate Set refill rate and units: RFR rate units Rate is of format: f f f f f f Units are one of: Definition µl/mn µl/hr...
  • Page 41 Pump Chain Commands DIA [<float>] Request to set or query syringe diameter setting. Set diameter: DIA diameter Diameter is of format: f f f f f f Units are MM. INFUSE and REFILL rates will be set to zero and AUTO FILL will be set to off.
  • Page 42 Pump Chain Commands MOD [{PMP|VOL|PGM}] Request to set or query pumping mode Set: MOD PMP (Puts pump in Pump Mode) MOD VOL (Puts pump in Volume Mode) MOD PGM (Puts pump in Program Mode) Command will not be accepted if the pump is running and a ‘Not Applicable’...
  • Page 43 Pump Chain Commands SYR [<float>] Request to set or query syringe volume setting for auto fill. Used in conjunction with Auto Fill feature. (See AF command). Set syringe volume: SYR volume Volume is of format: f f f f f f Units are ML.
  • Page 44 Pump Chain Commands SEQ [<integer>} [<entry>] Request to set or query programming sequences. <integer> is sequence number. Default is Sequence 1. Command only applicable while pump is stopped. Valid sequence numbers, ‘n’, are 1 to 10. ‘n’ defaults to 1 wherever it is optional. See program examples.
  • Page 45 Pump Chain Commands Query data item of program sequence n: Command Description SEQ [n] RAT Query rate Response: f f f f f f units Where units is one of the following: ml/mn ul/mn ml/hr ul/hr SEQ [n] GOT Query go to sequence number Response: <n>...
  • Page 46 Command not applicable at this time Control data is out of the operating range of the pump Note: If assistance is needed in programming pump control via RS-232C, call Harvard Apparatus Customer Service Support at 1-800-272-2775. Outside of U.S. call 508-893-8999. Publication 5406-001-REV-D...

  • Page 47: Appendices: A Table Of Syringe Diameters

    Appendix A: Syringe Diameters in mm Stainless Steel Terumo SGE Scientific Size Diameter Size Diameter Glass 8 cc 9.525 mm 3 cc 8.95 mm Size Engineering Diameter 19.130 13.00 28.600 15.80 25 µl 0.73 mm 34.900 20.15 1.03 44.755 23.10 1.46 29.10 2.30...

  • Page 48: Stainless Steel Syringe

    S S t t e e e e l l S S y y r r i i n n g g e e s s 100 ml Harvard now offers a completely new 50 ml line of Stainless Steel Syringes intended...
  • Page 49 O-Ring seals, two Teflon back-up rings and a choice of 1/16 or 1/8 syringe to tube fitting. When used in the MA1-70-2200 or MA1- 70-2201 Harvard PHD 4400, pressures up to of 1500 psi can be achieved for chro- matography and process control applications.

  • Page 50: C Nominal Min/Max Flow Rates

    For all types of custom pumping or pilot plant applications, the pump’s programming capab b i i l l ities can be enhanced. Please contact the Harvard Development Group if we can be of help.

  • Page 51: E Pressure And Force Specifications

    R R a a t t e e S S t t a a l l l l P P S S I I This is the rate indicated with a Harvard This is the back pressure that finally stalls Apparatus 50 ml stainless steel syringe.

  • Page 52: Grs-232C Specifications

    PHD 4400 Hpsi pump. Catalog Number Product MA1-70-2200 PHD 4400 Hpsi Programmable Syringe Pump MA1-70-2201 PHD 4400 Hpsi Remote Programmable Syringe Pump MA1-70-3000 Symphony Software...

  • Page 53: I User I/O Connector Specifications

    Appendix I: User I/O Connector Specs User I/O (input/output) connections are via a 9 pin D-sub type connector. All inputs are internally pulled up to a logic high. P P i i n n A A s s s s i i g g n n m m e e n n t t s s 1.

  • Page 54: K Maintenance And Troubleshooting

    Appendix K: Maintenance & Troubleshooting M M a a i i n n t t e e n n a a n n c c e e PHD 4400 Hpsi series pumps require no special maintenance other than keeping them clean by avoiding accidental spills of pumped material.

  • Page 55: L 'Auto-Fill' Valves

    Appendix L: ‘Auto Fill’ Valves There are two 'Auto Fill' Valve models available: Catalog No. Product 55-7002 Supplied with 1/8 inch I.D., 1/4 inch O.D. silastic tubing and connector to syringe 55-1145 Supplies with 1/4 inch O.D. 304 stainless steel tubing with Swagelok fittings S S e e t t t t i i n n g g U U p p t t h h e e V V a a l l v v e e Set up consist of three phases: Voltage selection (110 or 220 V), Pump to valve electri- cal connection and Valve tubing to syringe hook up.

  • Page 56: Declaration Of Conformity

    Declaration of Conformity Application of Council Directive(s): 73/23/EEC, 89/336/EEC Standard(s) to which conformity is declared: EN61010, EN50082-1, EN50081-1 Manufacturer’s Name: Harvard Apparatus, Inc. Manufacturer’s Address: 84 October Hill Road Holliston, Massachusetts 01746 U.S.A. Type of Equipment: Syringe Pump Model No.:...

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