HORIBA ABX PENTRA 80 RAA022AA Technical Manual
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HORIBA ABX PENTRA 80 RAA022AA Technical Manual

Large capacity autoloader (100 tubes)
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  • Page 1 Technical Manual P/n: RAA022B...
  • Page 2 PENTRA TECHNICAL MANUAL RAA022BA 1. Revisions Table 1: Table of Revisions Index P/N revision Revision Section Date RAA022AA RAH912AA < V1.01 31/03/02 RAA022B RAH986 XL 80 integration 30/04/04 This document applies to the latest higher software version. When a subsequent software version changes the information in this document, a new issue will be released.
  • Page 3 INTRODUCTION 2. Working conditions 2.1. Environment The Pentra should be operated in an indoor location only. Operation at an altitude over 3000 meters (9800 feet) is not recommended. Instrument is designed to be safe for transient voltages according to INSTALLATION CATEGORY II and POLLUTION DEGREE 2. Please ask your Diagnostics representative service center for any information about the operating location when it does not comply with the recommended specifications.
  • Page 4 PENTRA TECHNICAL MANUAL RAA022BA...

  • Page 5: Table Of Contents

    Specifications Section 1: Specifications 1. Technical specifications ..........1-2 1.1. Parameters..............1-2 1.2. Throughput Analyses ........... 1-2 1.3. Tube identification ............1-2 1.4. Reagents ..............1-3 1.5. Internal Computer ............1-3 1.6. Measurements and computation ........1-3 2. Physical specifications..........1-3 2.1. Power requirements............. 1-3 2.2.

  • Page 6: Technical Specifications

    PENTRA TECHNICAL MANUAL RAA022B 1. Technical specifications 1.1. Parameters Table 1: CBC Mode White Blood Cell Red Blood Cell Hemoglobin Concentration Hematocrit Mean Corpuscular Volume Mean Corpuscular Hemoglobin MCHC Mean Corpuscular Hemoglobin Concentration Red Distribution Width Platelets Platelets Distribution Width Mean Platelet Volume Plateletcrit Table 2: CBC + 5DIFF Mode...

  • Page 7: Reagents

    SECTION 1 SPECIFICATIONS 1.4. Reagents • ABX DILUENT (20 Litres). • ABX CLEANER (1 Litre, Integrated). • ABX EOSINOFIX (1 Litre, Integrated). • ABX BASOLYSE (1 Litre, Integrated). • ABX LYSE (0.4 Litre, Integrated). 1.5. Internal Computer • Color LCD touch screen: 12 inches. •...

  • Page 8: Minimum Specimen Volume

    PENTRA TECHNICAL MANUAL RAA022B 2.4. Minimum specimen volume • CBC Mode (CBC) ...........30µl. • CBC + 5DIFF Mode (DIF)........53µl. 2.5. dilution ratios • WBC/BASO..........1/200. • LMNE ............. 1/80. • RBC/PLT ..........1/10000. • HGB ............. 1/250. 2.6. Hgb measurement •...

  • Page 9: Summary Of Performance Data

    SECTION 1 SPECIFICATIONS STARTUP cycle estimated duration and consumptions are given for one blank cycle control. It could be a maximum of three cycles. 3. Summary of performance data 3.1. Repeatability • Based on 10 consecutive samples of the same fresh whole blood sample without alarm: Table 4: Repeatibility PARAMETERS Test Level...

  • Page 10: Reagents Specification

    PENTRA TECHNICAL MANUAL RAA022B 4. Reagents specification In order for the instrument to operate correctly, hight-quality reagents must be used. ABX DIAGNOSTICS provides all the necessary reagents. All these reagents have been registered by the A.F.S.S.A.P.S. «Agence Française de Sécurité Sanitaire des Produits de Santé»...

  • Page 11: Abx Cleaner

    SECTION 1 SPECIFICATIONS • Description: Colorless, odorless. • Physico-chemical properties: pH: 6.65 • Handling Precautions: May be dangerous. Avoid contact with eyes, skin and clothing. Wear laboratory gloves when handling the product. The product may be harmful if ingested. • Mesuring Principles: See user manual.

  • Page 12: Waste Handling Precautions

    PENTRA TECHNICAL MANUAL RAA022B • Mesuring Principles: See user manual. • Performances: See user manual. • Results: See user manual. • Directions for use: See user manual. • Emergency First Aid: If the eyes or skin come into contact with the reagent, rinse with water. If the reagent is inhaled, breath fresh air immediately.
  • Page 13 SECTION 1 SPECIFICATIONS RBC: • Red Blood Cells (Erythrocytes): The red blood cell dilution contains all the formed elements in the blood: erythrocytes, leukocytes and platelets. During erythrocytes counting (red blood cells), platelets are not counted as their size falls below the minimum threshold. •...
  • Page 14 PENTRA TECHNICAL MANUAL RAA022B platelets may exceed the upper threshold for the platelet parameter and are not counted. • Chemotherapy - Cytotoxic and immunosuppressive drugs may increase the fragility of these cells which may cause low Plt counts. Reference (manual) methods may be necessary to obtain an accurate platelet count.
  • Page 15 Hydraulic & Pneumatic principles Section 2: Hydraulic & Pneumatic principles 1. Hydropneumatic connections........2-2 2. Instrument tubing ............2-5 2.1. Tube list ..............2-5 2.2. Connectors and integrated tubings list ......2-7 3. Function of valves ...........2-7 4. Hydraulic circuit .............2-8 4.1. Cleaner circuit ............. 2-9 4.2.
  • Page 16 PENTRA TECHNICAL MANUAL RAA022B 1. Hydropneumatic connections Read this table as follow: LV3_2 means connection to port 2 of valve 3. Table 1: Hydropneumatic connection Circuit From F.S. Diameter Length T.S. To Atmosphere 2.05 LV14_2 LV14_1 1.52 Count syringe_1 Rinse chamber_1 - 2.05 LV17_2 Atmos.
  • Page 17 SECTION 2 HYDRAULIC & PNEUMATIC PRINCIPLES Table 1: Hydropneumatic connection Circuit From F.S. Diameter Length T.S. To LV20_2 1.52 T7_1 LV23_2 1.52 Probe rinse block_2 LV22_2 1.02 Sample syringe_2 LV11_2 1.52 LV25_3 LV25_1 1.52 LV27_3 LV27_1 1.52 LV26_3 LV26_1 1.52 Reagent heater_7 Reagent heater_8 1.52...
  • Page 18 PENTRA TECHNICAL MANUAL RAA022B Table 1: Hydropneumatic connection Circuit From F.S. Diameter Length T.S. To LMNE flow 1.52 E1_1 anode fitting cell_output E1_2 anode fitting 1.52 Isolator_1 Isolator_2 1.52 E2_1 ground fitting E2_2 ground fitting 1.52 LV28_2 LV28_1 1.52 T6_2 T6_1 1.52 Reagent heater_5...
  • Page 19 SECTION 2 HYDRAULIC & PNEUMATIC PRINCIPLES 2. Instrument tubing 2.1. Tube list Table 2: Tube list Designation Part Number Diameter Length Quantity Comment Y Connector EAB021A Y Connector EAB026A T410-6 Connector EAB033A T220-6 Connector EAB035A 0.040 TYGON EAE005A 1.02 tubing 1.02 1.02 1.02...
  • Page 20 PENTRA TECHNICAL MANUAL RAA022B Table 2: Tube list Designation Part Number Diameter Length Quantity Comment 1.52 1.52 1.52 1.52 1.52 1.52 1.52 1.52 1.52 1.52 0.081 TYGON EAE008A 2.05 tubing 2.05 Notched tubing 2.05 Notched tubing 2.05 Notched tubing 2.05 2.05 2.05 2.05...
  • Page 21 SECTION 2 HYDRAULIC & PNEUMATIC PRINCIPLES 2.2. Connectors and integrated tubings list The following table shows the connectors and integrated tubings list: Length x Quantity per diameter. Table 3: Connectors and integrated tubings list Designation Part Number Diameter Length Comment Y Connector EAB021A Y Connector...
  • Page 22 PENTRA TECHNICAL MANUAL RAA022B Table 4: Function of valves Valve Subject Functions number Cleaner Control cleaner entry\exit reagent syringe Eosinofix Control eosinofix entry\exit reagent syringe Diluent Control alphalyse entry\exit reagent syringe Diluent ouput con- Routes diluent to probe rinse block or heating coil trol Basolyse II Control basolyse II entry\exit reagent syringe...
  • Page 23 SECTION 2 HYDRAULIC & PNEUMATIC PRINCIPLES 4.1. Cleaner circuit 9/18...
  • Page 24 PENTRA TECHNICAL MANUAL RAA022B 4.2. Basolyse 2 circuit 10/18...
  • Page 25 SECTION 2 HYDRAULIC & PNEUMATIC PRINCIPLES 4.3. Eosinofix circuit 11/18...
  • Page 26 PENTRA TECHNICAL MANUAL RAA022B 4.4. Lyse circuit 12/18...
  • Page 27 SECTION 2 HYDRAULIC & PNEUMATIC PRINCIPLES 4.5. Diluent circuit 13/18...
  • Page 28 PENTRA TECHNICAL MANUAL RAA022B 4.6. Diluent prime circuit 14/18...
  • Page 29 SECTION 2 HYDRAULIC & PNEUMATIC PRINCIPLES 4.7. Counting circuit 15/18...
  • Page 30 PENTRA TECHNICAL MANUAL RAA022B 4.8. LMNE transfer circuit 16/18...
  • Page 31 SECTION 2 HYDRAULIC & PNEUMATIC PRINCIPLES 4.9. LMNE injection circuit 17/18...
  • Page 32 PENTRA TECHNICAL MANUAL RAA022B 5. Pneumatic diagram See pneumatic diagram on next page. 18/18...
  • Page 33 DIAGNOSTICS...
  • Page 35 Electric & Electronic principles Section 3: Electric & Electronic principles 1. Main board .............3-3 1.1. Main board general view ..........3-3 2. Motor board ............3-4 2.1. Motor board general view..........3-4 3. Connectors and other boards........3-4 3.1. CBC008A Barcode reader..........3-5 3.2.
  • Page 36 3.29. XBA432A Infrared photocell 600mm. (2 tabs)..3-33 3.30. XBA433A Infrared photocell 600mm. (1 tab) ... 3-34 3.31. XBA458A Switch JST 300mm........3-35 3.32. XBA459A Switch JST 400mm........3-36 3.33. XBA460A Switch Jst 500mm........3-37 3.34. XBA461A Switch Jst 700mm........3-38 3.35.
  • Page 37 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 1. Main board 1.1. Main board general view Diag.1:Main board general view 3/56...
  • Page 38 PENTRA TECHNICAL MANUAL RAA022B 2. Motor board 2.1. Motor board general view Diag.2:Motor board general view 3. Connectors and other boards 4/56...
  • Page 39 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.1. CBC008A Barcode reader Diag.3:CBC008A Barcode reader 5/56...
  • Page 40 PENTRA TECHNICAL MANUAL RAA022B 3.2. DAD075AS Flat cable 350mm. 14pts Diag.4:DAD075AS Flat cable 350mm. 14pts 6/56...
  • Page 41 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.3. DAD107AS Flat cable 480mm. 14pts Diag.5:DAD075AS Flat cable 480mm. 14pts 7/56...
  • Page 42 PENTRA TECHNICAL MANUAL RAA022B 3.4. DAD109A Flat cable 560mm. 14pts Diag.6:DAD109A Flat cable 560mm. 14pts 8/56...
  • Page 43 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.5. DAD112AS Flat cable 930mm. 26pts (Main board\Carriage board) Diag.7:DAD112AS Flat cable 930mm. 26pts 9/56...
  • Page 44 PENTRA TECHNICAL MANUAL RAA022B 3.6. DAD119AS Flat cable 950mm. 8pts Diag.8:DAD119AS Flat cable 950mm. 8pts 10/56...
  • Page 45 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.7. DAD120A Flat cable 800mm. 14pts Diag.9:DAD120A Flat cable 800mm. 14pts 11/56...
  • Page 46 PENTRA TECHNICAL MANUAL RAA022B 3.8. DAD121A Flat cable 700mm. 44pts Diag.10:DAD121A Flat cable 700mm. 44pts 12/56...
  • Page 47 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.9. DAD122A Flat cable 650mm. 14pts Diag.11:DAD122A Flat cable 650mm. 14pts 13/56...
  • Page 48 PENTRA TECHNICAL MANUAL RAA022B 3.10. DAD123A DSUB 25pts Flat cable 1000mm. Diag.12:DAD123A DSUB 25pts Flat cable 1000mm. 14/56...
  • Page 49 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.11. DAD124A DSUB 9pts Flat cable 1000mm. Diag.13:DAD124A DSUB 9pts Flat cable 1000mm. 15/56...
  • Page 50 PENTRA TECHNICAL MANUAL RAA022B 3.12. XAA428C Carriage board Diag.14:XAA428C Carriage board 16/56...
  • Page 51 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.13. XAA429A LEDs board Diag.15:XAA429A LEDs board 17/56...
  • Page 52 PENTRA TECHNICAL MANUAL RAA022B 3.14. XAA456A Main board Diag.16:XAA456A Main board 18/56...
  • Page 53 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.15. XAA458A LMNE amplifier board Diag.17:XAA458A LMNE amplifier board 19/56...
  • Page 54 PENTRA TECHNICAL MANUAL RAA022B 3.16. XAA459A Motor board Diag.18:XAA459A Motor board 20/56...
  • Page 55 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.17. XAA478A Solenoid board Diag.19:XAA478A Solenoid board 21/56...
  • Page 56 PENTRA TECHNICAL MANUAL RAA022B 3.18. XBA322B External waste level detection Diag.20:XBA322B External waste level detection 22/56...
  • Page 57 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.19. XBA342A Infrared sensor Diag.21:XBA342A Infrared sensor 23/56...
  • Page 58 PENTRA TECHNICAL MANUAL RAA022B 3.20. XBA387A Reagent heating coil Diag.22:XBA387A Reagent heating coil 24/56...
  • Page 59 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.21. XBA389A Hemoglobin photometer Diag.23:XBA389A Hemoglobin photometer 25/56...
  • Page 60 PENTRA TECHNICAL MANUAL RAA022B 3.22. XBA393A Upper fan Diag.24:XBA393A Upper fan 26/56...
  • Page 61 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.23. XBA396A Infrared 260mm. Diag.25:XBA396A Infrared 260mm. 27/56...
  • Page 62 PENTRA TECHNICAL MANUAL RAA022B 3.24. XBA398B RBC&WBC electrode coaxial cable Diag.26:XBA398B RBC&WBC electrode coaxial cable 28/56...
  • Page 63 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.25. XBA399B LMNE flowcell coaxial cable Diag.27:XBA399B LMNE flowcell coaxial cable 29/56...
  • Page 64 PENTRA TECHNICAL MANUAL RAA022B 3.26. XBA425A Chamber heating Diag.28:XBA425A Chamber heating 30/56...
  • Page 65 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.27. XBA427A Emergency position assy Diag.29:XBA427A Emergency position assy 31/56...
  • Page 66 PENTRA TECHNICAL MANUAL RAA022B 3.28. XBA431A Infrared photocell 600mm. (No tab) Diag.30:XBA431A Infrared photocell 600mm. (No tab) 32/56...
  • Page 67 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.29. XBA432A Infrared photocell 600mm. (2 tabs) Diag.31:XBA432A Infrared photocell 600mm. (2 tabs) 33/56...
  • Page 68 PENTRA TECHNICAL MANUAL RAA022B 3.30. XBA433A Infrared photocell 600mm. (1 tab) Diag.32:XBA433A Infrared photocell 600mm. (1 tab) 34/56...
  • Page 69 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.31. XBA458A Switch JST 300mm. Diag.33:XBA458A Switch JST 300mm. 35/56...
  • Page 70 PENTRA TECHNICAL MANUAL RAA022B 3.32. XBA459A Switch JST 400mm. Diag.34:XBA459A Switch JST 400mm. 36/56...
  • Page 71 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.33. XBA460A Switch Jst 500mm. Diag.35:XBA460A Switch Jst 500mm. 37/56...
  • Page 72 PENTRA TECHNICAL MANUAL RAA022B 3.34. XBA461A Switch Jst 700mm. Diag.36:XBA461A Switch Jst 700mm. 38/56...
  • Page 73 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.35. XBA462A Motor Jst 300mm. Diag.37:XBA462A Motor Jst 300mm. 39/56...
  • Page 74 PENTRA TECHNICAL MANUAL RAA022B 3.36. XBA463A Motor Jst 400mm. Diag.38:XBA463A Motor Jst 400mm. 40/56...
  • Page 75 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.37. XBA464A Motor Jst 500mm. Diag.39:XBA464A Motor Jst 500mm. 41/56...
  • Page 76 PENTRA TECHNICAL MANUAL RAA022B 3.38. XBA465A Motor Jst 700mm. Diag.40:XBA465A Motor Jst 700mm. 42/56...
  • Page 77 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.39. XBA469A Horizontal carriage cell extension Diag.41:XBA469A Horizontal carriage cell extension 43/56...
  • Page 78 PENTRA TECHNICAL MANUAL RAA022B 3.40. XBA470A Solenoid JST 700mm. Diag.42:XBA470A Solenoid JST 700mm. 44/56...
  • Page 79 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.41. XBA471A Solenoid Molex 230mm. Diag.43:XBA471A Solenoid Molex 230mm. 45/56...
  • Page 80 PENTRA TECHNICAL MANUAL RAA022B 3.42. XBA478A Horizontal carriage motor Diag.44:XBA478A Horizontal carriage motor 46/56...
  • Page 81 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.43. XBA486A LEDs extension JST 1000mm. Diag.45:XBA486A LEDs extension JST 1000mm. 47/56...
  • Page 82 PENTRA TECHNICAL MANUAL RAA022B 3.44. XBA487A LED JST 200mm. Diag.46:XBA487A LED JST 200mm. 48/56...
  • Page 83 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.45. XBA488A Waste level detection Diag.47:XBA488A Waste level detection 49/56...
  • Page 84 PENTRA TECHNICAL MANUAL RAA022B 3.46. XDA605A Diluent reservoir with level detection Diag.48:XDA605A Diluent reservoir with level detection 50/56...
  • Page 85 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.47. XDA751C 12 valves assy (LV1-6 & LV7-12) Diag.49:XDA751C 12 valves assy (LV1-6 & LV7-12) 51/56...
  • Page 86 PENTRA TECHNICAL MANUAL RAA022B 3.48. XDA752C 7 valves assy (LV13-19) Diag.50:XDA752C 7 valves assy (LV13-19) 52/56...
  • Page 87 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.49. XDA753C Carriage valves assy (LV20-23) Diag.51:XDA753C Carriage valves assy (LV20-23) 53/56...
  • Page 88 PENTRA TECHNICAL MANUAL RAA022B 3.50. XDA754C 7 valves assy (LV24-30) Diag.52: XDA754C 7 valves assy (LV24-30) 54/56...
  • Page 89 SECTION 3 ELECTRIC & ELECTRONIC PRINCIPLES 3.51. XDA755C 5 valves assy (LV31-35) Diag.53:XDA755C 5 valves assy (LV31-35) 4. Pentra 80 synoptic See synoptic diagram on next page. 55/56...
  • Page 91 Analysis cycle technology Section 4: Analysis cycle technologie 1. Analysis cycle description (Principles) .....4-2 2. Measuring principles ..........4-4 2.1. Multi distribution sytem (MDSS) ........4-4 2.2. CBC detection principles ..........4-5 2.3. WBC and differential count ......... 4-7...
  • Page 92 PENTRA TECHNICAL MANUAL RAA022B 1. Analysis cycle description (Principles) • Aspiration of blood sample from the tube trough the cap (53µl) • Translation of sampling carriage over Rinse chamber. Cleaning of the needles: • Internal cleaning: 1ml inside piercing needle •...
  • Page 93 SECTION 4 ANALYSIS CYCLE TECHNOLOGY • Translation of sampling carriage over Rinse chamber • Rinsing of the needles: • Internal rinsing: 0.8ml inside sampling needle • External rinsing: 0.5ml outside sampling needle • Internal rinsing: 0.5ml inside sampling needle • External rinsing: 0.3ml outside sampling needle WBC and LMNE counting: •...
  • Page 94 PENTRA TECHNICAL MANUAL RAA022B 2. Measuring principles 2.1. Multi distribution sytem (MDSS) 2.1.1. CBC mode In CBC mode, 30µl of whole blood is aspirated then delivered with reagents into chambers as follows: one specimen for the first RBC/Plt dilution and the Hgb measurement. another specimen for the BASO/WBC count.
  • Page 95 SECTION 4 ANALYSIS CYCLE TECHNOLOGY 2.2. CBC detection principles 2.2.1. RBC/Plt Measurement impedance variation generated by the passage of cells through a calibrated micro aperture. The specimen is diluted in an electrolytic diluent (current conductor) and pulled through the calibrated micro-aperture. Two electrodes are placed on either side of the aperture.
  • Page 96 PENTRA TECHNICAL MANUAL RAA022B Table 1: RBC\Plt dilutions Technical characteristics of the RED BLOOD CELL and PLATELET counts Final dilution rate** 1/10000 Count vacuum 200 mb Temperature of reaction 35°C Count period 2 X 6 seconds **: Two successive dilutions are carried out : Primary Dilution for RBC and Plt: Blood (µl) 10 µl Vol.
  • Page 97 SECTION 4 ANALYSIS CYCLE TECHNOLOGY 2.2.4. MCV, MCH, MCHC calculation • MCV (Mean Cell Volume) is calculated directly from the RBC histogram. • MCH (Mean Cell Hemoglobin) is calculated from the Hgb value and the RBC number. • The mean hemoglobin weight in each RBC is given by the formula: MCH (pg) = Hgb/RBC x 10 •...
  • Page 98 PENTRA TECHNICAL MANUAL RAA022B 2.3.2. BASO/WBC Count Detection principle is the same as for RBC. Differentiation betwen BASOs and other leukocy- tes is obtained by means of the BASOLYSE II specific lysing action. All the WBCs are counted between the electrical threshold <0>...
  • Page 99 SECTION 4 ANALYSIS CYCLE TECHNOLOGY 2.3.3. LMNE Matrix The WBC and Differential count are based on 3 essential principles: • The double hydrodynamic sleeving «DHSS» (ABX DIAGNOSTICS patent) • The volume measurement (impedance changes). • The measurement of transmitted light with 0°...
  • Page 100 PENTRA TECHNICAL MANUAL RAA022B No cell in the flowcell Baseline Poorly-stained (agranular) cell in the flowcell absorbance Hyper segmented with High complex granularity absorbance staining Diag.10:Absorbance measurement Results From these measurements, a matrix is drawn up with volumes on the X-axis and optical transmission on the Y-axis.
  • Page 101 SECTION 4 ANALYSIS CYCLE TECHNOLOGY EOSINOPHILS: With reagent action on cytoplasmic membranes, the leukocytes keep their native size and only eosinophils are colored for optical separation. Eosinophils will be situated in the upper part of the optical Y-axis due to their strong absorbance qualities and their size, which is nearly equivalent to large neutrophils.
  • Page 102 PENTRA TECHNICAL MANUAL RAA022B 12/12...
  • Page 103 Software release Section 5: Software release 1. Service software overview ........5-2 1.1. Super User Menu............5-2 1.2. Miniclean ..............5-3 1.3. Concentrated Cleaning ..........5-3 1.4. Autoclean ..............5-3 1.5. Technician Menu ............5-3 2. Windows Explorer Access ........5-4 3. Software releases .............5-4 3.1.
  • Page 104 PENTRA TECHNICAL MANUAL RAA022B 1. Service software overview 1.1. Super User Menu • Mechanical • Hydraulic • Others 1.1.1. Mechanical • Initialisation • Check Motors • Check Valves • Check Sensors • Sampler test • Holder Adjustment • Rack Adjustment 1.1.2.
  • Page 105 SECTION 5 SOFTWARE VERSIONS 1.2. Miniclean • Miniclean 1.3. Concentrated Cleaning • Concentrated Cleaning 1.4. Autoclean • Autoclean 1.5. Technician Menu • Measurement • Gains • Others 1.5.1. Measurement • Gain Adjustment • LMNE Adjustment • Aperture Current • Pulse Adjustment...
  • Page 106 PENTRA TECHNICAL MANUAL RAA022B 1.5.2. Gains • Thermic Adjustment • M.D.S.S. Adjustment • Liquid Sensor Adjustment • Vacuum Control • Sampler Adjust. • Bubbling 1.5.3. Others • LMNE Calibration • Cycles • Calibration Coefficients • Blank Values • System Tools •...
  • Page 107 Alarms & error list Section 6: Alarms & error list 1. Analyzer error types and help messages ....6-2 2. Transfer error types an help messages......6-3 3. STAT mode error type and help messages....6-4 4. Environment error type and help messages ....6-4 5.
  • Page 108 PENTRA TECHNICAL MANUAL RAA022B 1. Analyzer error types and help messages Table 1: Analyzer error types and help messages Alarm Error type Help message Carriage Motor home switch Run an Auto Clean Carriage motor failure always detected Check Motor in Service Menu Carriage mechanism not rea- Carriage Motor Mechanism ini- Run an Auto Clean...
  • Page 109 SECTION 6 ALARMS & ERROR LIST Table 1: Analyzer error types and help messages Alarm Error type Help message Thermostated Compartment Thermostated Compartment temperature out of range. Value Run an initialization Temperature Min. & Max. 2. Transfer error types an help messages Table 2: Transfer error types and help messages Alarm Error type...
  • Page 110 PENTRA TECHNICAL MANUAL RAA022B 3. STAT mode error type and help messages Table 3: STAT mode error types and help messages Alarm Error type Help message Tube holder mechanism failure Door not open No message 4. Environment error type and help messages Table 4: Environment error types and help messages Alarm Error type...
  • Page 111 SECTION 6 ALARMS & ERROR LIST Table 4: Environment error types and help messages Alarm Error type Help message Result not stored Software Run an autoclean RS232 alarm RS232 external problem Check host connection Sample ID %d already in pro- Software No message gress...
  • Page 112 PENTRA TECHNICAL MANUAL RAA022B 7. Analyzer internal barcode error types and help messages Table 7: Analyzer internal barcode error types and help messages Alarm Error type Help message Com error on slave %d Communication error with slave Run an Initialization Error management failed Unkwon cycle Run an Initialization...
  • Page 113 Maintenance Section 7: Maintenance 1. Maintenance ............7-2 1.1. Introduction..............7-2 1.2. Daily customer maintenance ........7-2 1.3. Weekly customer maintenance........7-2 1.4. Maintenance table ............7-2 2. Maintenance kits .............7-3 2.1. 6 month maintenance kit ..........7-3 2.2. Yearly maintenance kit ..........7-3 2.3.
  • Page 114 PENTRA TECHNICAL MANUAL RAA022B 1. Maintenance 1.1. Introduction Customer maintenance has to be carried out according to the recommended frequency chart table and after having attended an ABX approved customer training course. The system warranty may be affected if damage occurs after a non trained technician intervenes or if replaced spare parts and consumables do not come from an ABX approved origin.
  • Page 115 SECTION 7 MAINTENANCE 2. Maintenance kits 2.1. 6 month maintenance kit Table 2: 6 Month maintenance kit XEA710BS Designation FAA013A O’ring 1.80x1.20 Nitrile ( Rinsing block ) FAA055A O’ring 1.07x1.27 Fluocarbon ( Rinsing block ) FAA057A O’ring 5x1 Nitrile ( Rinsing block ) FAA065A O’ring 6.30x2.40 Silicon ( Reagent syringe ) XDA621A...
  • Page 116 PENTRA TECHNICAL MANUAL RAA022B 2.4. Fitting kit XEA311 kit includes most common pneumatic and hydraulic parts. 2.5. Screws kit XEA293 kit includes most common screws used in Pentra 80. 3. Maintenance procedures Maintenance and adjustments required for Pentra 80 are divided into procedures according to the concerned assemblies.
  • Page 117 SECTION 7 MAINTENANCE 3.2. Procedures chart table Procedure Procedure Concerns Designation Unpacking - Working conditions - Instrument installation - First start RAS324 Installation of the instrument Rinsing block - Reagent syringe - Draining syringe 1&2 - Counting RAS325 6 month maintenance syringe - Filter Sampling syringe O’ring - 5DIFF syringe O’ring - Draining chambers RAS326...
  • Page 118 PENTRA TECHNICAL MANUAL RAA022B Procedure Procedure Concerns Designation PC CD-ROM drive repla- RAS352 Replacement of the computer’s CD-ROM drive cement PC touch screen replace- RAS353 Replacement of the computer’s touch screen ment Instrument main board RAS354 Replacement of the instrument’s main board replacement RAS355 Motor board replacement Replacement of the motor board...
  • Page 119 SECTION 7 MAINTENANCE 3.3. Training department procedure list Procedure Procedure Concerns Designation Unpacking - Working conditions - Instrument installation - First start RAS324 Installation of the instrument Front panel & Covers dis- RAS342 Dismantling of all instrument’s panels and covers mantling Rinsing block - Reagent syringe - Draining syringe 1&2 - Counting RAS325...
  • Page 120 PENTRA TECHNICAL MANUAL RAA022B Procedure Procedure Concerns Designation Optical bench dismantling RAS341 Optical bench dismantling, replacement and control & replacement External barcode reader RAS344 Adjustment of external barcode configuration RAS345 Heater assy replacement Replacement of the reagent heating system Instrument main board RAS354 Replacement of the instrument’s main board replacement...
  • Page 121 Installation Procedure RAS324B Installation procedure • Concerns • Working conditions • Instrument installation • First start of the instrument • Required tools None • Required products None • Intervention time 1 hour • Frequency On request • Specific kit or consumables Installation kit: XEA785B Disposal gloves and white coat must be worn by the operator.
  • Page 122 PENTRA TECHNICAL MANUAL INSTALLATION 1. Working conditions 1.1. Environment The Pentra should be operated in an indoor location only. Operation at an altitude over 3000 meters (9800 feet) is not recommended. Instrument is designed to be safe for transient voltages according to INSTALLATION CATEGORY II and POLLUTION DEGREE 2.
  • Page 123 PROCEDURE RAS324B After unpacking check that all of the parts from the package list are present. Table 1: Package list Part Number Designation XEA785B Installation kit Pentra 80 XBA453A Barcode reader GBD072A Lifting handles GBL0280 Rack 10 vials 13x82 CBK044A Computer Mouse GBL0250 Keyboard drawer...
  • Page 124 PENTRA TECHNICAL MANUAL INSTALLATION Table 2: Installation Kit XEA785B Part Number Designation MAB018A Allen bent key 3mm MAB090A Torx bent key T10 XDA483B 3V liquid valve (without self) XDA621A O’ring 30,8x3,8 + Washer XDA692A Diluent input tubing XEA018A Diluent straw Lg=360mm XEA019A Grease XBA322B...
  • Page 125 PROCEDURE RAS324B 2.2. Transport and installation To operate the instrument correctly, install it in a place satisfaying the following conditions: • Place where the instrument and reagents are not thermally affected by direct sunlight and intense radiant heat. • Place where it is not exposed to water or vapor. •...
  • Page 126 PENTRA TECHNICAL MANUAL INSTALLATION Remove the Left panel door then the Upper cover to access to the optical bench. For transport, the optical bench is fixed on the frame with holding plates. They have to be removed before starting the instrument and fixed as shown on next diagram (See “Holding plates”, page 6).
  • Page 127 PROCEDURE RAS324B Mouse Keyboard Diag.5 Computer’s connections FRONT 1- VGA 6- Printer 2- Mouse 7- COM3: External Barcode reader 3- Keyboard 8- COM2: Labo link 4- LAN 9- COM1: To Mother Board 5- USB 10- Power Diag.6 Computer connectors Route the computer’s wiring under the frame of the Pentra (See “Computer’s wiring”, page 7).
  • Page 128 PENTRA TECHNICAL MANUAL INSTALLATION 2.4. Reagents installation Use «Luer» connectors, straws and stoppers supplied with the installation kit to connect integrated reagents, diluent and wastes. Bottles and container locations (See “Reagent location”, page 8): 1- ABX Lyse 2- ABX Basolyse II 3- ABX Eosinofix 4- ABX Cleaner 5- ABX Diluent...
  • Page 129 PROCEDURE RAS324B Barcode of rack Type of rack (Number & Type) Diag.9 Rack’s labels front side Diag.10 Rack’s labels rear side 3.2. Check keyboard setup Check the type of the keyboard used (Qwerty or Azerty). From window Input Locales, menu: Settings\System\Local Settings, press Edit button then Change Input Locales button.
  • Page 130 PENTRA TECHNICAL MANUAL INSTALLATION 3.6. Mechanic check Make sure all the panels are fitted on the instrument. Check in menu: Service\Super User\Mechanical\Check Sensors, that all the sensors are green. Check rack is well transfered to the reception tray, menu: Service\Technician\Gains\Sampler adjust.
  • Page 131 6 month maintenance RAS325B 6 month maintenance • Concerns • Rinsing block • Reagent syringe • Draining syringe 1 • Draining syringe 2 • Counting syringe • Instrument & Filter cleaning • Required tools • Hexagonal Keys • Dynamometric screw drivers A302, A301, A300 •...
  • Page 132 PENTRA TECHNICAL MANUAL PROCEDURE Table 1: 6 Month maintenance kit XEA710BS Designation FAA013A O’ring 1.80x1.20 Nitrile ( Rinsing block ) FAA055A O’ring 1.07x1.27 Fluocarbon ( Rinsing block ) FAA057A O’ring 5x1 Nitrile ( Rinsing block ) FAA065A O’ring 6.30x2.40 Silicon ( Reagent syringe ) XDA621A O’ring 30.80x3.60 + washer ( counting + draining syringe 1 &...
  • Page 133 6 MONTH MAINTENANCE RAS325B Diag.2 locker. Use a small amount silicon grease with FAA057A O’ring (See Diag.3“O’ring position.”, page 3) and replace the O’rings from the rinsing block Diag.3 O’ring position. Reassemble in reverse order. Do not tight too much the rinsing block screws. 2.
  • Page 134 PENTRA TECHNICAL MANUAL PROCEDURE • valve 7 inlet 3. • valve 8 inlet 3. • valve 9 inlet 3. • valve 10 inlet 3. • valve 12 inlet 3. Diag.4 Reagent syringe Unscrew the 2 fixation screws and remove carefully the reagent syringes (See Diag.4“Reagent syringe”, page 4).
  • Page 135 6 MONTH MAINTENANCE RAS325B • 1_ Reagent syringe body (GBG033a). • 2_XDA622A. • 3_Piston ( GBC030A). • 4_FX M3x12 screws. • 5_CHC M3x12 screws. • 6_Reagent syringe bottom plate (GBG034A). • 7_Hgb lyse reagent piston (GBC031A). • 8_O’ring (FAA065A). Diag.6 Reagent syringe view. XDA622A = O’ring (FAA063A) + Washer (GBG149A).
  • Page 136 PENTRA TECHNICAL MANUAL PROCEDURE Put a piece of absorbant paper under the syringe and disconnect all the tubes from the syringe. Remove the syringe. Drain the syringe pushing the piston nut up and down several times. Open the syringe: - 4x CHC M4x16 screws on old model - Rotation of the Syringe Body on new model ( See Diag.8“Draining 1 syringe opening.”, page 6).
  • Page 137 6 MONTH MAINTENANCE RAS325B 3.2. O’ ring replacement on draining syringe 2. Switch off the instrument and disconnect power supply cable. Remove cover (See RAS342 procedure). Disconnect electrical connectors on the syringe then unscrew the 4 CHC M4x16 syringe screws, just few turns to release the silent blocks (See Diag.10“Draining 2 syringe dismantling.”, page 7).
  • Page 138 PENTRA TECHNICAL MANUAL PROCEDURE 4. Instrument cleaning. Concerned assemblies All the thermostated compartment. Outer surfaces of the instrument (perpex, covers, reagent locations..). Waste connector plug. Liquid valve push button. Assemblies close to the needle. Tube holder assembly. Overflow trays. Procedure Dilute the 12°cl bleach to 1 part of bleach for 4 of deionize water (1/5).
  • Page 139 Yearly maintenance Procedure RAS326B Yearly maintenance • Concerns • Samplig needle replacement • Sampling syringe: O’ring replacement • 5DIFF syringe: O’ring replacement • Draining chambers: O’ring replacement • Optical bench: Lamp & LMNE coaxial replacement • Diluent reservoir • Counting chambers •...
  • Page 140 PENTRA TECHNICAL MANUAL PROCEDURE 1. Yearly maintenance kit Table 1: Yearly maintenance kit XEA486CS Designation DAJ007A Optical bench lamp FAA040A O’ring 12.1x2.7 5DIFF syringe FAA046A O’ring 2.75x1.6 Coaxial cable FAA064A O’ring 14.2x1.52 Sampling syringe FAA066A O’ring 13.1x1.6 Draining chambers FAA067A O’ring 2.4x1.9 5DIFF syringe GBG275A Counting head EPO 0.5 joint...
  • Page 141 YEARLY MAINTENANCE RAS326B 3. Sampling syringe O’ring replacement (2xFAA064A) Switch the instrument off and disconnect power supply cable. Open the righthand side door. Move the carriage to access to the sampling syringe. Disconnect the tubes from the sampling syringe and unscrew the 2 fixation screws (See Diag.2 “Sampling syringe dismantling”, page 3).
  • Page 142 PENTRA TECHNICAL MANUAL PROCEDURE 4. 5DIFF syringe O’ring replacement (FAA040A & 4xFAA067A) Open the lefthand side panel to access to the 5DIFF syringe. Gently disconnect the tubes from the 5DIFF syringe and unscrew the 2 fixation screws (See Diag.3 “5DIFF syringe dismantling”, page 4).
  • Page 143 YEARLY MAINTENANCE RAS326B 5. Optical bench lamp replacement (DAJ007A) Before dismantling optical bench lamp make sure it is cold. Do not touch lamp bulb with the fingers, use a piece of cloth or paper to manipulate the lamp. Open the cover (see RAS342 Front panel & Covers dismantling procedure for further details). Disconnect the lamp supply (See Diag.5 “Optical bench lamp disconnection”, page 5).
  • Page 144 PENTRA TECHNICAL MANUAL PROCEDURE 6. LMNE flowcell coaxial replacement (XEA663A) Open the optical bench cover (See Diag.7 “Optical bench cover dismantling”, page 6). Diag.7Optical bench cover dismantling Disconnect coaxial connector from the Optical bench board (See Diag.8 “Coaxial disconnection”, page 6). Disconnect the coaxial from the LMNE flowcell (See Diag.8 “Coaxial disconnection”, page 6).
  • Page 145 YEARLY MAINTENANCE RAS326B 7. Optical bench lamp 6V voltage check Run a Rinse cytometer cycle to get ride of air bubbles stuck to the inner optical surfaces. From menu Service\Menu Super User\Hydraulic\Clean cycle\Rinse cytometer (). Check that the flowcell contains no or just a very few air bubbles. From menu Service\Menu technician\Measurment\LMNE adjustment Click on Run LMNE Adjustment button.
  • Page 146 PENTRA TECHNICAL MANUAL PROCEDURE 8. Diluent reservoir Joint & Washer replacement (XEA286AS) From menu: Services\Super User Menu\Hydraulic\Unprime Cycle run the All option to drain the chambers and the diluent reservoir. Remove the righthand side panel and the mother board cover from the instrument (See procedure RAS342 Front panel &...
  • Page 147 YEARLY MAINTENANCE RAS326B 9. Draining chambers O’ring replacement (4xFAA066A) Make sure the chambers are drained, if not run a Services\Super User Menu\Hydraulic\Unprime Cycle\All command to drain the chambers. Put a piece of absorbant paper or cloth under the chambers assembly. Turn gently the chamber’s bottom between 2 fingers (See Diag.13 “Draining chamber dismantling”,...
  • Page 148 PENTRA TECHNICAL MANUAL PROCEDURE 10. Counting heads RBC\WBC coaxial cable O’ring replacement (2xFAA046A) Make sure the chambers are drained, if not run a Services\Super User Menu\Hydraulic\Unprime Cycle\All command to drain the chambers. Switch the instrument off. Put a piece of absorbant paper or cloth under the chambers assembly. Record the tube positions before dismantling the chambers assembly.
  • Page 149 YEARLY MAINTENANCE RAS326B 11. Counting head aperture joint replacement (2xGBG275A) Carefully dismantle the counting head (See Diag.19 “Counting heads dismantling”, page 11) and plunge the aperture in distilled water. Diag.19Counting heads dismantling Replace EPO joints by new ones (See Diag.20 “Counting heads aperture joint”, page 11).
  • Page 150 PENTRA TECHNICAL MANUAL PROCEDURE 12. Tape on trays If the Sliding bands located on Loading and Ejection trays are damaged, replace them using the Tape (JAJ007A) included in the kit (See Diag.21 “Sliding bands”, page 12). Use Acetone to clean the trays. 310 mm 320 mm 220 mm...
  • Page 151 Piston kit replacement Procedure RAS327B Piston kit replacement • Concerns Pentra maintenance procedure • Sampling probe: Percutor replacement • Reagents syringe: Pistons replacement • Vacuum\waste pump: Piston replacement • Required tools • Hexagonal Keys • Dynamometric screw drivers A302, A301, A300 •...
  • Page 152 PENTRA TECHNICAL MANUAL PROCEDURE 1. Piston & Needle kit Table 1: Piston & Needle kit XEA711CS Designation GBC030A Reagent syringe Piston GBC031A Reagent syringe Piston GBG260A Piston D=31 Spacer GBG274A Piston D=31 KAA009A CHC M3x20 Screws GBG169A Percutor This procedure must be performed on a clean instrument.If the instrument is suspected not to be perfectly clean, perform a concentrated cleaning.
  • Page 153 PISTON KIT REPLACEMENT RAS327B Diag.2Lift locker Replace the percutor (See Diag.3“Rinsing block unit”, page 3). GBG169A Diag.3Rinsing block unit Use new O’rings when performing 6 month maintenance at the same time. Change the needle (XDA655DS) when performing Yearly maintenance at the same time. Do not tight too much the rinsing block screws (Torque: 120 mN.m \ 17 Ozf.in).
  • Page 154 PENTRA TECHNICAL MANUAL PROCEDURE Diag.4Reagent syringe dismantling Unscrew the 2 fixation screws and remove carefully the reagent syringe (See Diag.4“Reagent syringe dismantling”, page 4). Push several times the piston over the waste container to drain the syringes. On a piece of absorbant paper open the reagent syringe: 9x CHC M3x12 screws, 2x FX M3x12 screws.
  • Page 155 PISTON KIT REPLACEMENT RAS327B 2.3. Draining syringe 1 piston replacement (GBG274A & GBG260A) Switch the instrument off and disconnect power supply cable. Remove the cover and the righthand side door to access to Draining syringe 1 (See RAS342 Front panel & covers dismantling procedure for further details). Disconnect electrical connectors from the syringe, then unscrew the 4 syringe screws, just few turns to free silent block (See Diag.6“Draining 1 syringe electrical...
  • Page 156 PENTRA TECHNICAL MANUAL PROCEDURE Prepare the piston: On the new molded syringe, the piston (1) is shorter (See Diag.8“Pistons”, page 6). For new molded syringe, install only the piston GBG274A (1) For old machined syringe, use the piston GBG274A (1) + the cross piece GBG260A (2) and use the CHC M3x20 screws from the kit.
  • Page 157 PISTON KIT REPLACEMENT RAS327B 2.4. Draining syringe 2 piston replacement (GBG274A & GBG260A) Switch the instrument off and disconnect power supply cable. Remove the cover and the lefthand side panel to access to the reagent syringe (See RAS342 Front panel & Covers dismantling procedure for further details). Disconnect electrical connectors from the syringe then unscrew the 4 syringe’s screws, just few turns to free the silent blocks (See Diag.10“Draining syringe 2...
  • Page 158 PENTRA TECHNICAL MANUAL PROCEDURE 3. Instrument cleaning Concerned assemblies: • All the thermostated compartment. • Outer surfaces of the instrument (perpex, covers, reagent locations..). • Waste connector plug. • Liquid valve push button. • Assemblies close to the needle. • Tube holder assembly.
  • Page 159 Decontamination and rinse Procedure RAS328B Decontamination & rinse • Concerns Instrument decontamination before maintenance operations in the following cases: • Instrument removed from biohazardous area • Maintenance intervention on contaminated assemblies • Required tools • Hexagonal keys • Clamps • Flat scrw driver •...
  • Page 160 PENTRA TECHNICAL MANUAL PROCEDURE 1. Preparation Switch the instrument on. Open the thermal door (Righthand side of the instrument). Perform an Concentrated Cleaning from menu: Service\Menu SuperUser\Hydraulical\Clean Cycles. Confirm the concentrated cleaning by pressing Ok button. When asked during the concentrated cleaning cycle, poor 3ml of Minoclair in each chamber, then click ok to continue.
  • Page 161 DECONTAMINATION AND RINSE RAS328B cycles. Fill a sample tube with diluted bleach to 1 part of bleach for 4 parts of deionize water (1/5). Run 15 Burn-in cycles from menu: Service\Menu Technician \Others\Burn-in cycle. Enter 15 cycles in Total Cycles To Do then press button Start rack (See “Burn-in cycle screen”, page 3).
  • Page 162 PENTRA TECHNICAL MANUAL PROCEDURE Press the «Validate» button then repeat this cycle a second time. Dry the straws using absorbant paper. Plunge the straws in a bottle full of distilled water. Enter: Service \ Super User Menu \ Hydraulic \ Prime Cycles then click the «ALL REAGENTS» button (See “Prime cycles screen”, page 4).
  • Page 163 Chambers Adjustment Procedure RAS329B Chambers adjustment • Concerns Chambers check and alignment • Required tools Allen keys • Required products None • Intervention time 15 min. • Frequency On request • Specific kit or consumables None Disposal gloves and white coat must be worn by the operator. Local or national regulations must be applied in all the operations.
  • Page 164 PENTRA TECHNICAL MANUAL PROCEDURE It is mandatory to perform the Probe adjustment procedure after this adjustment. 1. Alignment check and adjustment This adjustment procedure must be carried out when chamber assembly is moved or replaced. Switch off the instrument and disconnect power supply cable. Remove cover (See RAS342) Move the carriage over the rinse chamber, then gently push down on the probe until it touch the top of the rinse bath(See Diag.1...
  • Page 165 CHAMBERS ADJUSTMENT RAS329B Diag.2 WBC chamber position Check that there is the same height between probe and Rinse and WBC/Baso tops of chambers. If you need to get a better position, free the chamber support by loosening the 5 screws.(See Diag.3 “Chamber support screws”, page 3).
  • Page 166 PENTRA TECHNICAL MANUAL PROCEDURE...
  • Page 167 Probe Adjustment Procedure RAS330B Probe adjustment • Concerns Check and adjustment of the probe • Required tools Gauge set MAJ004A • Required products None • Intervention time 15min • Frequency On request • Specific kit or consumables None Disposal gloves and white coat must be worn by the operator. Local or national regulations must be applied in all the operations.
  • Page 168 PENTRA TECHNICAL MANUAL PROCEDURE 1. MDSS adjustment menu Check that chamber assy is parallel to the carriage motion before checking or making an adjustment of the Probe position. Follow CHAMBERS ADJUSTMENT procedure of this manual (RAS329AA). Probe adjustments can be accessed by: «Menu\Service\Menu Technician\Gains\MDSS adjustment»(See Diag.1 “MDSS adjustment menu”, page 2).
  • Page 169 PROBE ADJUSTMENT RAS330B Diag.2 Check needle home Check that the probe is about 1mm out from the percing needle (See Diag.3 “Needle home position”, page 3). Piercing needle 1 mm Sampling needle Diag.3 Needle home position Press «OK» to continue (See Diag.4 “Check needle position button”, page 3).
  • Page 170 PENTRA TECHNICAL MANUAL PROCEDURE A Right position B Wrong position Remove cover (See RAS342). Enter «Menu\Service\Menu Technician\Gains\MDSS adjustment» (See Diag.1 “MDSS adjustment menu”, page 2). Press «Check Needle Position» button (See Diag.5 “Check needle position”, page 4). Diag.5 Check needle position The sampling needle moves over the LMNE chamber.
  • Page 171 PROBE ADJUSTMENT RAS330B Diag.8 Check tubing Press «OK» to continue. 2.4. Needle position adjustment Check needle position (See 2.3. “Needle position check”, page 3). If probe is too low, increase Needle position number of step. If probe is too hight, decrease Needle position number of step. Run the «Check Needle Position»...
  • Page 172 PENTRA TECHNICAL MANUAL PROCEDURE Check the gap between the tooth wheel and the front of the carriage then press «OK» at the following screen (See Diag.11 “Check needle position button”, page 6). Diag.11 Check needle position button If the «Carriage home» position need to be adjust, change the «Carriage home» value then press the «Check Carriage Home»...
  • Page 173 PROBE ADJUSTMENT RAS330B 3.2. Carriage position check Enter «Menu\Service\Menu Technician\Gains\MDSS adjustment» Press «Check Carriage Position» button (See Diag.12 “Check carriage position button”, page 7). Diag.12 Check carriage position button The sampling needle moves over a chamber. Check the probe position as describe above (See Diag.6 “Needle perfect position”, page 4).
  • Page 174 PENTRA TECHNICAL MANUAL PROCEDURE Diag.15 Carriage positions values If you want to modify only one of the position, remind to increase or decrease the next motion with the same number of steps, not to loose the other adjustments See Diag.16 “Chambers”, page 8). Diag.16 Chambers 8/10...
  • Page 175 PROBE ADJUSTMENT RAS330B 4. Further adjustment Use a 0.15 mm gauge (1) between the Piercing block (A) and the White safety block (B) to adjust the collar (C) blocking the carriage vertical movement (See Diag.17 “Collar adjustment”, page 9). Diag.17Collar adjustment When the carriage is at the «Home position»...
  • Page 176 PENTRA TECHNICAL MANUAL PROCEDURE 10/10...
  • Page 177 Main Board Adjustment Procedure RAS331B Main board adjustment • Concerns Main board adjustment: • How to access to Main board • Main board general view • Main board supply check • Main board adjustment • Required tools • Allen keys •...
  • Page 178 PENTRA TECHNICAL MANUAL PROCEDURE 1. How to access Main board • Remove the righthand side cover of the instrument: • Open the righthand side front cover. • Remove the thermal panel. • Unscrew the 9 screws from the righthand side cover and carefully remove the cover. •...
  • Page 179 MAIN BOARD ADJUSTMENT RAS331B 2. Main board general view Diag.1Main board general view...
  • Page 180 PENTRA TECHNICAL MANUAL PROCEDURE 3. Main board check 3.1. Power supply check The power supply test points are located at the bottom of the Main board and are not adjustable (See “Power supply test points”, page 4) GROUND Diag.2Power supply test points Table 1: Power supply voltages check (Ground on TP47) Voltage Test Point...
  • Page 181 MAIN BOARD ADJUSTMENT RAS331B 4. Main board adjustment Remove the righthand side cover in order to access to the main board. 4.1. Hgb blank adjustment Make sure the thermal door is closed. Run command Hgb Blank Adjustment from menu: Service\Technician\Measurement\Gain. The utility carries out a cycle a rinsing cycle of the WBC chamber and continuously displays the voltage used by the converter.
  • Page 182 PENTRA TECHNICAL MANUAL PROCEDURE 4.3. WBC\Baso gain adjustment A special cycle allows the dilution of the RBC\Plt LATEX to 1/733rd (3µl of LATEX with 2.2ml of diluent). A special count program carries out the calculation of the mean volume in the predefined zones and displays them every 700ms.
  • Page 183 MAIN BOARD ADJUSTMENT RAS331B 4.5. Motor current adjustment The motor current test points are located in the middle of the Main board, on the left (See “Motor test points”, page 7). Sampling syringe: TP66 - R114 Carriage: TP65 - R126 Diag.7Motor test points Check and adjust if necessary the motor current according to the following table.
  • Page 184 PENTRA TECHNICAL MANUAL PROCEDURE...
  • Page 185 Motor Board Adjustment Procedure RAS332B Motor board adjustment • Concerns Motor board adjustment: • How to access to Motor board • Motor board general view • Motor board check and adjustment • Required tools • Allen keys • Voltmeter • Required products None •...
  • Page 186 PENTRA TECHNICAL MANUAL PROCEDURE 1. How to access to the Motor board The motor board is located under the sampler loarder system on the lefthand side of the instrument. • Lift up the reagent cover and remove the bottles. • Remove the plastic protection under the reagents.
  • Page 187 MOTOR BOARD ADJUSTMENT RAS332B 2. Motor board general view Diag.1Motor board general view...
  • Page 188 PENTRA TECHNICAL MANUAL PROCEDURE 3. Motor board check and adjustment Table 1: Motor board check and adjustment target values Motor Test Point Potentiometer Target Reagent syringe 4V ±0.05V Optical bench syringe 3V ±0.05V Counting syringe 4,8V ±0.05V for Molded Syringe (New)* 4,0V ±0.05V for Machined Syringe (Old) Draining syringe #2 4,8V ±0.05V for Molded Syringe (New)*...
  • Page 189 Temperature Adjustment Procedure RAS333B Temperature adjustment • Concerns Reagent heating coil and Thermostatic compartment temperature adjustment. • Required tools • Thermometer • Required products None • Intervention time 30 min. • Frequency On request • Specific kit or consumables None Disposal gloves and white coat must be worn by the operator.
  • Page 190 PENTRA TECHNICAL MANUAL PROCEDURE 1. Reagent Heating Coil Check & Adjustment Start the instrument. Enter menu Service\Technician\Measurement\Gain. The following screen is displayed (See “Thermic adjustment window”, page 2). Diag.1Thermic adjustment window Reagent Heating: • Reference CTN value: Input the CTN constant in this field. •...
  • Page 191 TEMPERATURE ADJUSTMENT RAS333B Make sure the thermometer sensor is deeply plunged into the liquid. After thermometer probe is installed, close the thermal door. Enter in Run Cycles Number field (in the Reagent heating area of the window), 5 cycles to run. Press Check Thermic (in the Reagent heating area of the window) button to start control.
  • Page 192 PENTRA TECHNICAL MANUAL PROCEDURE This control must be made with the thermal door closed and under stabilised thermic conditions (At least 5 minutes after startup). Open the thermal door on the righthand side of the instrument. Put the thermometer sensor in the CTN sensor (See “Thermostatic compartment thermometer’s sensor installation”, page 4).
  • Page 193 TEMPERATURE ADJUSTMENT RAS333B • If Temperature recorded is decreased, reagents preheating temperature decreases. Make a Thermostatic compartment temperature check after the adjustment as descibed at the previously in this procedure. If required change the Temperature recorded value and check temperature again until you get an acceptable temperature.
  • Page 194 PENTRA TECHNICAL MANUAL PROCEDURE...
  • Page 195 Vacuum Adjustment Procedure RAS334B Vacuum adjustment • Concerns Counting and draining 1 & 2 syringes vacuum control and adjustement • Required tools • Allen keys • Barflex • Flat screws drivers • Required products None • Intervention time 15 min. •...
  • Page 196 PENTRA TECHNICAL MANUAL PROCEDURE 1. Draining syringe vacuum check (No adjustment available). 1.1. Draining syringe 1. Remove cover (See RAS342 procedure). Disconnect the tube on the draining syringe 1 (inlet 1) and replace it by the Barflex tube. (See Diag.1 “Draining syringe 1”, page 2).
  • Page 197 VACUUM ADJUSTMENT RAS334B Diag.3 Validate Disconnect the Barflex and connect the tube back on the draining syringe 1. If vacuum is not stable or too low, check vacuum circuit (tubings, valves,syringe or O’ring) and repair if necessary. 1.2. Draining syringe 2. Disconnect the tube on the draining syringe 2 (inlet 1) and replace it by the Barflex tube.
  • Page 198 PENTRA TECHNICAL MANUAL PROCEDURE 2. Counting syringe vacuum check and adjustment. 2.1. Counting syringe vacuum check. Remove cover (See RAS342 procedure). Disconnect the lowest tube (inlet 4) on the counting syringe and replace it by the barflex tube. (See Diag.5 “Counting syringe”, page 4).
  • Page 199 VACUUM ADJUSTMENT RAS334B Diag.6 Check and validate Press OK button. Disconnect the Barflex and connect the tube back on the counting syringe. 2.2. Counting syringe vacuum adjustment. Counting syringe vacuum check» procedure (See above). Follow the « Change the Vacuum value (motor step). If vacuum is too low, increase step value. Repeat vacuum check and step value adjustment until you get the correct vacuum (200mb).
  • Page 200 PENTRA TECHNICAL MANUAL PROCEDURE...
  • Page 201 Bubbling Adjustment Procedure RAS335B Bubbling adjustment procedure • Concerns Bubbling adjustment. • Required tools None • Required products None • Intervention time 15 min. • Frequency On request • Specific kit or consumables None Disposal gloves and white coat must be worn by the operator. Local or national regulations must be applied in all the operations.
  • Page 202 PENTRA TECHNICAL MANUAL PROCEDURE Bubbling is factory adjusted and normally does not required further adjustment. If an adjustment is necessary, follow this procedure. Enter Menu\Service\Menu Technician\Gains\Bubbling (See Diag.1 “Bubbling”, page 2). Diag.1 Bubbling Enter new step value for each bubbling, and click «Accept Values». To increase bubbling, increase the number of steps.
  • Page 203 LMNE Flowcell Adjustment Procedure RAS336B LMNE flowcell adjustment • Concerns Instrument adjustment : LMNE Flowcell • Required tools • Allen keys • Flat screw driver • Required products • Latex • Fresh blood sample • Calibrator • Intervention time • Frequency On request •...
  • Page 204 PENTRA TECHNICAL MANUAL PROCEDURE 1. LMNE Flowcell adjustment check. This adjustment procedure must be carried out when LMNE flowcell is moved or replaced, or when LMNE tube is changed. No adjustment is required when LMNE lampe is replaced. Enter : Menu /Service/Menu technician/Measurment/LMNE adjustment allows the adjustment of the entire optical bench parameters.
  • Page 205 LMNE FLOWCELL ADJUSTMENT RAS336B Diag.2 Clean cycle Check that the flowcell contains no or just a very few air bubbles. Enter : Menu/service/menu technician/measurment/LMNE adjustment (See Diag.3 “LMNE adjustment”, page 3) Diag.3 LMNE adjustment Mix the RBC/PLT latex thorougly. Click on «Run LMNE Adjustment» button. Closed tube holder when the following screen appears :(See Diag.4 “Sample the latex”, page 4).
  • Page 206 PENTRA TECHNICAL MANUAL PROCEDURE Diag.4 Sample the latex When optical bench is well adjusted : (See Diag.5 “LMNE adjustment screen”, page 4) Diag.5 LMNE adjustment screen Check that the displayed values are within the range.(See Table 1: “TARGET VALUES”, page 4). Table 1: TARGET VALUES PARAMETER TARGET VALUE...
  • Page 207 LMNE FLOWCELL ADJUSTMENT RAS336B 2. LMNE Flowcell position adjustment Enter : Menu/service/menu technician/measurment/LMNE adjustment. Click on «Run LMNE Adjustment» button. Closed tube holder when the following screen appears :(See Diag.6 “Sample the latex”, page 5) Diag.6 Sample the latex During the cycle, adjust LMNE lamp voltage around 6V by turning R414 potentiometer, located on optical bench board.(See Diag.7 “Optical bench board”, page 5)
  • Page 208 PENTRA TECHNICAL MANUAL PROCEDURE R148 R136 Diag.8 Mother board Adjust Optical gain (LMNE ABS) to the maximum (and above 170). Adjustment is made by lateral or axial reception gun movement, use toothed wheel and lateral screw (See Diag.9 “Opical bench adjustment”, page 6).
  • Page 209 LMNE FLOWCELL ADJUSTMENT RAS336B Transfer time bargraph is used to ensure the correct Right/Left location of the light rectangle in the flowcell. When the optimal value is reached, the transfer time is around 200 because of the constant height betweens guns alignment and flowcell. This height is garanted by the washer under the flowcell, always put them back even when the flowcell is remplaced by a new one.
  • Page 210 PENTRA TECHNICAL MANUAL PROCEDURE...
  • Page 211 LMNE Balance Adjustment Procedure RAS337B LMNE balance adjustment • Concerns LMNE Balance calibration and forced calibration • Required tools None • Required products Fresh and normal blood samples (5 different samples at least) • Intervention time 15min • Frequency On request •...
  • Page 212 PENTRA TECHNICAL MANUAL PROCEDURE This procedure must be performed on a clean instrument. If the instrument is suspected to not be perfectly clean, perform a Concentrated cleaning. Enter Menu : Service/Menu technician/others/LMNE calibration.(See Diag.1 “LMNE menu”, page 2) Diag.1 LMNE menu Prepare a minimum of 5 different human blood samples without WBC alarm.
  • Page 213 LMNE BALANCE ADJUSTMENT RAS337B At least 5 results are necessary to calibrate the LMNE balance. Select results to be involved in the statistical calculation by clicking the checked box in «Sel» column (See Diag.4 “Old coef”, page 3). To discard a result from the statistical calculation, click the checked box again to remove the mark. Diag.4 Old coef Click on «auto calibration»...
  • Page 214 PENTRA TECHNICAL MANUAL PROCEDURE New LMNE Balance coefficient is applied to the instrument (See Diag.6 “New coef”, page 4). Diag.6 New coef LMNE balance coefficient can be modified in the software. Press «Calibration coefficients» button,change LMNE value, then press «Accept Values» button (See Diag.7 “Calibration coefficients”, page 4).
  • Page 215 Tube holder adjustment Procedure RAS338B Tube holder adjustment • Concerns Tube holder general view Tube holder assembly replacement Tube holder adjustments Needle adjustment Adjustment check Compatible tube list • Required tools • Allen keys • Flat screwdriver • Required products None •...
  • Page 216 PENTRA TECHNICAL MANUAL PROCEDURE 1. Tube holder general view Diag.1Tube holder exploded view 2. Tube holder assembly replacement Switch the instrument off. Remove the 2 front doors. Push electromagnet axis to free and open the tube holder, remove the tube holder. Cut the 2 tyraps and disconnect the electro-magnet command (See Diag.2“Tube holder dimantling ”, page 3).
  • Page 217 TUBE HOLDER ADJUSTMENT RAS338B Diag.2Tube holder dimantling Remove the tube holder assembly. Install the new tube holder assembly at the same place (Use the marks previously done). Tight gently tube holder fixations screws. Plug the electro-magnet command connector and replace the tyraps. Close the tube holder to sampling position and check the tube holder axis support touch the rail behind (See Diag.3“Tube holder pre-adjustment...
  • Page 218 PENTRA TECHNICAL MANUAL PROCEDURE 3. Tube holder adjsutments Switch the instrument on and log as «technician». From menu: Services\Technician menu\Gains\MDSS Adjustment\Holder Adjustment, press Control button. Put the tube holder back then close the door and check that the needle is correctly centered inside one of the tube holder’s sampling hole (Position 1 for example).
  • Page 219 TUBE HOLDER ADJUSTMENT RAS338B Diag.6Tube holder play adjustment If necessary adjust tube holder’s locker to get a correct play (See Diag.7“Tube holder locker ”, page 5). Diag.7Tube holder locker Without this play, because of the tube’s cap, the tube holder may not open correctly. Tight the locker screws when adjustment is correct.
  • Page 220 PENTRA TECHNICAL MANUAL PROCEDURE 4. Needle adjustment If the Teflon part of the needle comes into contact with the tube holder, the needle will be damaged. When the adjustment of the tube holder is completed, adjust the Tube Holder Position to get a correct needle sampling position.
  • Page 221 TUBE HOLDER ADJUSTMENT RAS338AA 7/10...
  • Page 222 PENTRA TECHNICAL MANUAL PROCEDURE 8/10...
  • Page 223 TUBE HOLDER ADJUSTMENT RAS338AA 9/10...
  • Page 224 PENTRA TECHNICAL MANUAL PROCEDURE 8. Compatible tube list for rack Part Piercing Manufacturer Model Additive Vol. Vaccum Type of cap number conditions Becton D Vacutainer 367651 K3-EDTA With cap Hemogard Becton D Vacutainer 367652 K3-EDTA With cap Hemogard Becton D Vacutainer 367654 K3-EDTA...
  • Page 225 Power supply replacement Procedure RAS339B Power supply replacement • Concerns Instrument dismantling : Power supply • Required tools • Hexagonal keys • Flat screw driver • Required products None • Intervention time 30 min • Frequency On request • Specific kit or consumables Power supply : DBN006A Disposal gloves and white coat must be worn by the operator.
  • Page 226 PENTRA TECHNICAL MANUAL PROCEDURE Switch off the instrument. Disconnect Power supply cable. Remove cover (See RAS342). Lift the bar up by unscrewing the right screw (See Diag.1 “Bar screw”, page 2). Diag.1 Bar screw Disconnect, on the Power supply, the supply connector (See Diag.2 “Power supply connector”, page 2).
  • Page 227 POWER SUP¨PLY REPLACEMENT RAS339B Diag.3 Power supply front screws Unscrew the 2 back screws of the Power supply (See Diag.4 “Power supply back screws”, page 3). Diag.4 Power supply back screws Remove the Power supply. Reassemble following previous steps backward. Switch on the instrument.
  • Page 228 PENTRA TECHNICAL MANUAL PROCEDURE Diag.5 Test points...
  • Page 229 Check up after intervention Procedure RAS340B Check up after intervention • Concerns Check up and control of the instrument accuracy: • Repeatability • Calibration • Control • Required tools None • Required products • Fresh and normal blood samples • Calibration blood samples •...
  • Page 230 PENTRA TECHNICAL MANUAL PROCEDURE 1. Repeatability This procedure must be performed on a clean instrument. If the instrument is suspected to not be perfectly clean, perform a Concentrated cleaning. The measurement of repeatability is based on the set of 10 results obtained from the consecutive analyses of the same blood sample.
  • Page 231 CHECK UP AFTER INTERVENTION RAS340B Diag.1Calibration window In the event that the result shows an analysis fault or reject, the result is not stored. An error message advising of rejection is diplayed. To discard a result from the statistical calculation, click into the Sel column the checked box to remove the result from the statistical calculation.
  • Page 232 PENTRA TECHNICAL MANUAL PROCEDURE Diag.2Control window...
  • Page 233 Optical bench Dismantling & Replacement Procedure RAS341B Optical bench dismantling & replacement • Concerns Optical bench: • Dismantling • Replacement • Control • Required tools • Hexagonal keys • Dynamometric screwdriver A302, A301, A300 • Flat screwdriver • Torx keys •...
  • Page 234 PENTRA TECHNICAL MANUAL PROCEDURE 1. Optical bench dismantling Switch the instrument off. Disconnect power supply cable. Remove the lefthand side vertical panel and the top cover to access to the optical bench. From the Optical bench disconnect the following cables (See Diag.1 “Optical bench dismantling (Ground, Ground fitting, Signal cable)”, page 2):...
  • Page 235 OPTICAL BENCH DISMANTLING & REPLACEMENT RAS341B Diag.3Optical bench lamp disconnection Remove the Optical bench electronic board, unscrew the 2 screws maintaining the board (See Diag.4 “Optical bench electronic board dismantling”, page 3): Diag.4Optical bench electronic board dismantling Untight the 4 fixation screws (silent-blocks) of the Optical bench on the frame (See Diag.5 “Optical bench fixation screws”, page 3.
  • Page 236 PENTRA TECHNICAL MANUAL PROCEDURE 2. Control of the Optical bench 2.1. Emission gun control of position Remove the Optical bench cover (See Diag.6 “Optical bench cover dismantling”, page 4): Diag.6Optical bench cover dismantling Check that gap between the emission gun and the flowcell is around 3mm. (See Diag.7 “Optical bench control”, page 4).
  • Page 237 OPTICAL BENCH DISMANTLING & REPLACEMENT RAS341B Diag.8Lamp control 1 Check that the projection of lamp filament is vertical on the piece of paper (See Diag.9 “Lamp control 2”, page 5). Diag.9Lamp control 2 A small screw (1.2mm. allen key allows adjustment of the lamp position (See Diag.10 “Lamp adjustment screw”, page 5).
  • Page 238 PENTRA TECHNICAL MANUAL PROCEDURE...
  • Page 239 Front panel & covers dismantling Procedure RAS342B Front panel & covers dismantling • Concerns Front panel & covers dismantling • Required tools • Hexagonal keys • Flat screw driver • Required products None • Intervention time 30min • Frequency On request •...
  • Page 240 PENTRA TECHNICAL MANUAL PROCEDURE Switch off the instrument. Disconnect power supply cable. Pull Front left cover down (1), then remove it (2). (See Diag.1 “Front left cover”, page 2). Diag.1 Front left cover Pull Front right cover down (1), then remove it (2). (See Diag.2 “Front right cover”, page 2).
  • Page 241 FRONT PANEL & COVERS DISMANTLING RAS342B Open the Right door, place the key inside the screw slot and turn the captive screw counterclockwise (See Diag.3 “Right door”, page 3). Diag.3 Right door Remove Right side cover by unscrewing the 9 CHC M4X8 (See Diag.4 “Right side cover”, page 3).
  • Page 242 PENTRA TECHNICAL MANUAL PROCEDURE Remove it with Reagent cover hinge by unscrewing 3 CHC M4X8 and 2 CHC M3X6 with washers (See Diag.5 “Reagent cover”, page 4). Diag.5 Reagent cover Remove Left side cover by unscrewing 6 CHC M4X6 (See Diag.6 “Left side cover”, page 4).
  • Page 243 FRONT PANEL & COVERS DISMANTLING RAS342B Remove Left Panel by unscrewing 2 CHC M4X6 at the front and loosening 2 CHC M4X6 at the rear (See Diag.7 “Left panel”, page 5). Diag.7 Left panel Remove Loading tray by unscrewing the 2 CHC M4X6 (A) and 1 FHC M4X6 and loosening 1 CHC M4X6 (B).
  • Page 244 PENTRA TECHNICAL MANUAL PROCEDURE Remove the 4 reagent bottles (disconnect tubes from the stoppers). Remove bottle recuperation tray. Remove the Rear panel by loosening 4 CHC M4X6 (See Diag.9 “Rear panel”, page 6). Diag.9 Rear panel Remove Bottle support by loosening 4 CHC M4X6 (See Diag.10 “Bottle support”, page 6).
  • Page 245 FRONT PANEL & COVERS DISMANTLING RAS342B Remove the Motor board protection 1 by loosening the 2 CHC M4X6 screws (See Diag.11 “Motor board protection 1”, page 7). Diag.11 Motor board protection 1 Remove the Motor board protection 2 by loosening the CHC M4X6 screws (See Diag.12 “Motor board protection 2”, page 7).
  • Page 246 PENTRA TECHNICAL MANUAL PROCEDURE Remove Ejection Tray by unscrewing 2 CHC M4X6 and 2 FHC M4X6 (See Diag.13 “Ejection tray”, page 8). Diag.13 Ejection tray Remove Upper Cover by unscrewing 6 CHC M4X6 screws : 2 on the right side, 2 on the left side and 3 at the rear of the instrument (See Diag.14 “Upper cover”, page 8).
  • Page 247 Internal barcode adjustment Procedure RAS343B Internal barcode adjustment • Concerns • Adjustment of the internal barcode reader. • Barcode configuration • Required tools • Allen keys • Flat screwdriver • Required products None • Intervention time 15 min. • Frequency On request •...
  • Page 248 PENTRA TECHNICAL MANUAL PROCEDURE 1. Barcode reader adjustment check Remove cover (see RAS342 procedure). Load a rack with 2 tubes fitted with the same large barcode sticker in position 1 and 2, to check the correct barcode reading operation. Enter: Menu\Service\Technician Menu\Gains\Sampler Adjustment. Put the rack on Loading Tray.
  • Page 249 INTERNAL BARCODE ADJUSTMENT RAS343B 2. Barcode reader adjustment If Rack label reading zone is not within bargraph zone, free the Barcode Reader Support Plate by loosening the two M3x6 CHC screws (See Diag.2“Barcode reader”, page 3), then move it to adjust reading position.
  • Page 250 PENTRA TECHNICAL MANUAL PROCEDURE 3. Barcode configuration 3.1. Typology and Ckeck Digit Several standards of barcode labels with different encoding are available. According to the model of barcode used by the laboratory, it is necessary to define the settings of the read characters. Following, the barcode standards the most used in laboratories: •...
  • Page 251 INTERNAL BARCODE ADJUSTMENT RAS343B • open the tab 2. • Select the «Code type = Code 128». • Check the «Check Digit = Not transmitted» box. • Confirm pressing «Accept values». • And then open the tab 3. • Select the «Code type = Codabar». •...
  • Page 252 PENTRA TECHNICAL MANUAL PROCEDURE...
  • Page 253 External barcode configuration Procedure RAS344B External barcode configuration. • Concerns External barcode reader test and configuration. • Required tools None • Required products None • Intervention time 15 min. • Frequency On request • Specific kit or consumables None Disposal gloves and white coat must be worn by the operator. Local or national regulations must be applied in all the operations.
  • Page 254 PENTRA TECHNICAL MANUAL PROCEDURE 1. Barcode reader configuration check Check that barcode reader is working properly by means of reading test. INTERLEAVED 2 OF 5 (no «check digit» control) with C.D. 1224488 reading12244881 without C.D. 121314151617 reading 121314151617 CODE 39 (no «check digit» control) with C.D.
  • Page 255 EXTERNAL BARCODE CONFIGURATION RAS344B 2. Barcode reader setup An audible beep occurs after each reading. Read all the labels from top to bottom and from left to right. When the last label is read, check once more on the test labels. For the code I2 of 5, to avoid a bad interpretation, it is mandatory to hold the reader in order to read the entire code.
  • Page 256 PENTRA TECHNICAL MANUAL PROCEDURE...
  • Page 257 Heater assy replacement Procedure RAS345B Heater assy replacement • Concerns Replacement of the reagent heating system. • Required tools • Hexagonal keys • Flat screwdriver • Required products None • Intervention time • Frequency On request • Specific kit or consumables XDA625AS Disposal gloves and white coat must be worn by the operator.
  • Page 258 PENTRA TECHNICAL MANUAL PROCEDURE 1. Heater assy dismantling Switch the instrument off and disconnect power supply cable. Remove the cover and open the rigthhand side panel (See procedure RAS342 Front panel & Cover dismantling for further details). Remove the lower part of thermostatic room (See Diag.1“Thermostatic room dismantling”, page 2).
  • Page 259 HEATER ASSY REPLACEMENT RAS345B Diag.3Heater disconnection Disconnect CTN from main board plug in J31 (See Diag.4“CTN disconnection”, page 3). Diag.4CTN disconnection Remove the heater. 2. Heater replacement Install the new heater the same way in reverse order. Use sleevings when necessary (Tubes connected to the heater for example). Make sure all the tubes are connected.
  • Page 260 PENTRA TECHNICAL MANUAL PROCEDURE...
  • Page 261 Mixer replacement Procedure RAS346B Mixer replacement • Concerns Replacement of the mixer system. • Required tools • Hexagonal keys • Flat screwdriver • Required products None • Intervention time • Frequency On request • Specific kit or consumables • PC extension cable: XEA723AS •...
  • Page 262 PENTRA TECHNICAL MANUAL PROCEDURE 1. Mixer assembly dismantling Turn off the instrument. Remove the power supply cable. Remove Covers (see RAS342B). Remove the internal PC (see RAS348B) and install the XEA723AS extension cable kit. 1.1. Top sampler transfer rail dismantling Before dismantling, mark with a pen the location of the rail on its support (See Diag.1“Rail location”, page 2).
  • Page 263 MIXER REPLACEMENT RAS346B 1.3. Plastic protection Remove the plastic protection located under the mixer by unscrewing the CHC screw (See Diag.4“Plastic protection”, page 3). Diag.4Plastic protection 1.4. Mixer disconnection Follow RAS332B (Motor board adjustment procedure) to access to the motor board. On the motor board, disconnect the following connectors: •...
  • Page 264 PENTRA TECHNICAL MANUAL PROCEDURE 2. Adjustments - If the Pentra 80 serial number is higher than P800154, no adjustments are necessary, except entering the new movement values as follow. • Plug the power supply cable then turn on the instrument. •...
  • Page 265 MIXER REPLACEMENT RAS346B - If the serial number is lower than P800154, check the following grabber positions procedure. • Check the Transfer mechanism home (see RAS347B - 3.1. Transfer home). • Check the «First transfer» movement (see RAS347B - 3.4. First transfer). •...
  • Page 266 PENTRA TECHNICAL MANUAL PROCEDURE...
  • Page 267 Automatic sampler RAS347B Automatic sampler • Concerns • Loading mechanism check & adjustment • Mixer mechanism check & adjustment • Transfer mechanism check & adjustment • Required tools None • Required products None • Intervention time • Frequency On request •...
  • Page 268 PENTRA TECHNICAL MANUAL PROCEDURE 1. Loading mechanism check & adjustment Remove cover except Loading Tray and Ejection Tray (See RAS342 procedure). Enter: Menu\Service\Technician Menu\Gains\Sampler Adjustment (See Diag.1 “Sampler adjustment menu”, page 2) Diag.1 Sampler adjustment menu Following adjustments must be checked and adjusted in the following order. Make sure to not damage the different switches by moving manually the racks.
  • Page 269 AUTOMATIC SAMPLER RAS347B SLIDE SLIDE SLIDE SLIDE SLIDE SLIDE SLIDE SLIDE RACK RACK RACK RACK front plate front plate front plate front plate Diag.2 Max rack loading gap Check the gap, then press validate button. If the gap (1) is too small (mechanical stop, noise), decrease «Max Rack Loading» value. If the gap (1) is too big, increase «Max Rack Loading»...
  • Page 270 PENTRA TECHNICAL MANUAL PROCEDURE Make sure that motor does not loose steps (noise) when Slides come against the rack, because it can return a little bit if motion is too big. If play (1) is too big : increase «Stop Rack Loading» value. If play (1) is too small : decrease «Stop Rack Loading»...
  • Page 271 AUTOMATIC SAMPLER RAS347B Hole RACK CARRIAGE FINGER Diag.6 Carriage position At the end of Transfer Carriage motion, Carriage motor is no more supplied. Make sure it does not move when you push finger up. Adjust Transfer Home value to center Carriage Finger into rack hole: If finger is on the right, increase «Transfer Home»...
  • Page 272 PENTRA TECHNICAL MANUAL PROCEDURE Stop Transfer Switch RACK Ejection Tray PLAY(~0.5mm) Diag.7 End of transfer Make sure that motor does not loose steps (noise) at the end of transfer motion. If rack is too much on right, decrease «Stop Transfer» value. If rack is too much on left, increase «Stop Transfer»...
  • Page 273 AUTOMATIC SAMPLER RAS347B EJECTION STOPS Diag.8 Rack ejection If rack is not out of «Ejection stops» increase «Rack Ejection Value». If rack is too much out of «Ejection stops», or at mechanichal stop, decrease «Rack Ejection Value». Repeat the procedure until you get the correct adjustment. 3.4.
  • Page 274 PENTRA TECHNICAL MANUAL PROCEDURE LEFT TRACER LEFT TRACER LEFT TRACER Rack motion Rack motion Rack motion RACK RACK RACK Diag.9 Tracers detection...
  • Page 275 Internal PC Replacement Procedure RAS348B Internal PC replacement • Concerns Dismantling of the internal PC • Required tools Allen keys • Required products None • Intervention time 10 min. • Frequency On request • Specific kit or consumables • For Pentra 80: - XAA553CS Kit •...
  • Page 276 PENTRA TECHNICAL MANUAL PROCEDURE 1. PC dismantling PC Hard Disk may contains some user’s specific datas (Worklist, Archives, etc...) if the PC is replaced these informations won’t be accessible. Read technical note included in the kit before replacing the PC assembly. Switch the instrument off and disconnect power supply cable.
  • Page 277 INTERNAL PC REPLACEMENT RAS348B Diag.2LED connector Untight the PC fixation screws (See Diag.3“Righthand side PC fixation screws”, page 3 & See Diag.4“Lefthand side PC fixation screws”, page 4). Diag.3Righthand side PC fixation screws...
  • Page 278 PENTRA TECHNICAL MANUAL PROCEDURE Diag.4Lefthand side PC fixation screws Lift and carrefully remove the PC. 2. PC installation Install the PC and tight the fixation screws (See Diag.3“Righthand side PC fixation screws”, page 3 & See Diag.4“Lefthand side PC fixation screws”, page 4).
  • Page 279 PC Hard Disk Replacement Procedure RAS349B PC Hard Disk replacement • Concerns PC Hard Disk replacement • Required tools Allen Keys • Required products None • Intervention time 1 hour • Frequency On request • Specific kit or consumables • For Pentra 80: - XAA510CS Kit.
  • Page 280 PENTRA TECHNICAL MANUAL PC HARD DISK REPLACEMENT PROCEDURE If it’s possible, save and/or print configuration before following this procedure (See RAS 357). Read technical note included in the kit before replacing the Hard disk. Remove internal PC (See RAS348). Unscrew the 6 CHC M4x6 screws to remove Internal PC from the cover. Unscrew the 4 CHC M3x6 and the 4 CHC M4x6 screws (See “Cover plate + fan”, page 2).
  • Page 281 PROCEDURE RAS349B Disconnect flat cable from Hard Disk, then from CD-ROM Drive (See “Flat cable disconnection”, page 3). Diag.3 Flat cable disconnection Disconnect FPC cable from Floppy disk Reader and remove Drives Support with Hard Disk, Floppy Disk Reader and CD-ROM Reader (See “Floppy disk reader connection”, page 3).
  • Page 282 PENTRA TECHNICAL MANUAL PC HARD DISK REPLACEMENT PROCEDURE Unscrew the 4 CHC M3x4 screws and remove Drives Support with Hard Disk (See “Drives support”, page 4). Diag.5 Drives support Return Drives Support and unscrew the 4 CHC M3x4 screws to free Hard Disk (See “hard disk screws”, page 4).
  • Page 283 PROCEDURE RAS349B Change Hard Disk. Hard Disk (reference XAA510AS) is formated and updated with last version of software. Reassemble in reverse order. Make sure that the flat cable connector is correctly plugged on Hard Disk because 4 pin are free (See “Hard disk connector”, page 5) and that blue band on FPC cable (Floppy disk reader...
  • Page 284 PENTRA TECHNICAL MANUAL PC HARD DISK REPLACEMENT PROCEDURE...
  • Page 285 PC Mother Board Replacement Procedure RAS350B PC Mother board replacement • Concerns PC Mother board replacement • Required tools • Allen Keys • Flat Screwdriver • Required products None • Intervention time 1 hour • Frequency On request • Specific kit or consumables Mother Board kit: XAA509BS Disposal gloves and white coat must be worn by the operator.
  • Page 286 PENTRA TECHNICAL MANUAL PC MOTHER BOARD REPLACEMENT PROCEDURE Unscrew the 6 CHC M4x6 screws to remove Internal PC from the cover. Unscrew the 4 CHC M3x6 and the 4 CHC M4x6 screws (See “Cover plate + fan”, page 2). Do not remove Left Angle Plate (A) in order to keep the PC/Cover adjustment. Diag.1 Cover plate + fan Disconnect fan and remove cover plate.
  • Page 287 PROCEDURE RAS350B page 3). Diag.3 Flat cable disconnection Before to disconnect FPC flat cable, pull gently on brown part of connector (1) to free the flat cable (See “Floppy disk reader connection”, page 3). Disconnect FPC cable (2) from Floppy disk Reader (See “Floppy disk reader connection”, page 3).
  • Page 288 PENTRA TECHNICAL MANUAL PC MOTHER BOARD REPLACEMENT PROCEDURE page 4). Diag.5 Mother board Unscrew the 4 CHC M4x10 screws (See “Mother board”, page 4). Remove TFT Board located under Mother board (See “TFT board”, page 4). Diag.6 TFT board Remove Mother Board and disconnect FPC flat cable (See “FPC flat cable connection”, page 5).
  • Page 289 PROCEDURE RAS350B pull gently on brown part of connector (1). pull gently on brown part of connector (1). Pull on FPC flat cable (2). Blue band of FPC cable in this position Diag.7 FPC flat cable connection Remove SDRAM Board (See “SDRAM disconnection”, page 5).
  • Page 290 PENTRA TECHNICAL MANUAL PC MOTHER BOARD REPLACEMENT PROCEDURE...
  • Page 291 PC Floppy Disk Reader Replacement Procedure RAS351B PC floppy disk reader replacement • Concerns PC floppy disk reader replacement • Required tools Allen Keys • Required products None • Intervention time 1 hour • Frequency On request • Specific kit or consumables Floppy disk reader:CBT014A Disposal gloves and white coat must be worn by the operator.
  • Page 292 PENTRA TECHNICAL MANUAL PC FLOPPY DISK READER REPLACEMENT Remove internal PC (See RAS348). Unscrew the 6 CHC M4x6 screws to remove Internal PC from the cover. Unscrew the 4 CHC M3x6 and the 4 CHC M4x6 screws (See “Cover plate + fan”, page 2).
  • Page 293 PROCEDURE RAS351B Diag.3 Flat cable disconnection Disconnect FPC cable from Floppy disk Reader (See “Floppy disk reader connection”, page 3). pull gently brown part of connector up (1). pull gently brown part of connector up (1). Pull up FPC flat cable (2). Blue band of Blue band of FPC cable in...
  • Page 294 PENTRA TECHNICAL MANUAL PC FLOPPY DISK READER REPLACEMENT Unscrew the 4 CHC M2,5x3 then remove Floppy Disk Reader (See “Floppy disk reader screws”, page 4). Diag.5 Floppy disk reader screws Change Floppy Disk Reader (Reference: CBT014A) and reassemble in reverse order. Make sure that FPC cable is correctly plugged with the blue band in correct position (See “Floppy disk reader connection”, page 3).
  • Page 295 PC CD-ROM Drive Replacement Procedure RAS352B PC CD-ROM drive replacement • Concerns PC CD-ROM drive replacement • Required tools • Allen Keys • Required products None • Intervention time 1 hour • Frequency On request • Specific kit or consumables CD-ROM Drive: CBT013A Disposal gloves and white coat must be worn by the operator.
  • Page 296 PENTRA TECHNICAL MANUAL PC CD-ROM DRIVE REPLACEMENT PROCEDURE Remove internal PC (See RAS348). Unscrew the 6 CHC M4x6 screws to remove Internal PC from the cover. Unscrew the 4 CHC M3x6 and the 4 CHC M4x6 screws (See “Cover plate + fan”, page 2).
  • Page 297 PROCEDURE RAS352B Disconnect flat cable from Hard Disk, then from CD-ROM Drive (See “Flat cable disconnection”, page 3). Diag.3 Flat cable disconnection Disconnect FPC cable from Floppy disk Reader (See “Floppy disk reader connection”, page 3). pull gently brown part of connector up (1). Pull up FPC flat cable (2).
  • Page 298 PENTRA TECHNICAL MANUAL PC CD-ROM DRIVE REPLACEMENT PROCEDURE Unscrew the 4 CHC M3x4 screws and remove Drives Support with Hard Disk (See “Drives support”, page 4). Diag.5 Drives support Unscrew the 4 CHC M2,5x3 then remove Floppy Disk Reader (See “Floppy disk reader screws”, page 4).
  • Page 299 PROCEDURE RAS352B Unscrew the 2 CHC M2x6 screws (See “CD-ROM board”, page 5) and remove board from CD- ROM drive (there is a nut between CD-ROM drive and board). Diag.8 CD-ROM board Change CD-ROM drive (Reference CBT013A) and reassemble in reverse order Make sure that FPC cable is correctly plugged with the blue band in correct position (See “Floppy disk reader connection”, page 3).
  • Page 300 PENTRA TECHNICAL MANUAL PC CD-ROM DRIVE REPLACEMENT PROCEDURE...
  • Page 301 PC Touch screen Replacement Procedure RAS353B PC Touch screen replacement • Concerns PC Touch screen replacement • Required tools Allen Keys • Required products None • Intervention time 1 hour • Frequency On request • Specific kit or consumables PC touch screen: XAA511AS Disposal gloves and white coat must be worn by the operator.
  • Page 302 PENTRA TECHNICAL MANUAL PC TOUCH SCREEN REPLACEMENT PROCEDURE 1. Touch screen dismantling Remove internal PC (See RAS348). Unscrew the 6 CHC M4x6 screws to remove Internal PC from the cover. Unscrew the 6 CHC M4x6 screws then remove the plate to access to the interface board (See “Touch screen interface board”, page 2).
  • Page 303 PROCEDURE RAS353B 2. New touch screen installation Put the new touch screen on the screen, the flat cable should be on the right side. On new instruments, a square located at the bottom of the screen allow to keep the vertical location of the touch screen.
  • Page 304 PENTRA TECHNICAL MANUAL PC TOUCH SCREEN REPLACEMENT PROCEDURE 3. Adjustment Turn on the instrument. Enter : Menu\ Service \ Technician menu \ Others \ System tools 3.1. For a Pentra 80 Press the «Launch Windows Explorer» button. Enter: Start \ Run then enter «Calwin.exe» in the window, and press «OK» (See “Run”, page 4). Diag.4Run A white window appears, with a red cross in the left upper corner.
  • Page 305 Instrument Mother Board Replacement Procedure RAS354B Instrument mother board replacement • Concerns Instrument mother board replacement • Required tools • Allen Keys • Voltmeter • Required products None • Intervention time 30 min • Frequency On request • Specific kit or consumables •...
  • Page 306 PENTRA TECHNICAL MANUAL INSTRUMENT MOTHER BOARD REPLACEMENT Before changing a Mother Board, make sure that Analyser Settings are saved on a floppy disk or on Hard disk. If not, follow RAS357 Procedure «Save and Restore Settings» to save and/or print Analyser Settings.
  • Page 307 PROCEDURE RAS354B 3. Mother Board Replacement: Install new Mother Board and screw the 9 CHC M4x 6 screws. Connect all the connectors on the board (See “Main board connections”, page 3). Diag.2Main board connections Switch on the instrument and adjust Mother Board (See RAS331: Main Board Adjustment). Restore settings (See RAS357 Save and Restore Settings and follow Technical note included in the kit).
  • Page 308 PENTRA TECHNICAL MANUAL INSTRUMENT MOTHER BOARD REPLACEMENT...
  • Page 309 Motor board replacement Procedure RAS355B Motor board replacement • Concerns Replacement of the motor electronic command board. • Required tools • Hexagonal keys • Voltmeter • Required products None • Intervention time 30min. • Frequency On request • Specific kit or consumables Motor board: XAA459BS Disposal gloves and white coat must be worn by the operator.
  • Page 310 PENTRA TECHNICAL MANUAL PROCEDURE 1. How to access to motor board The motor board is located under the sampler loarder system on the lefthand side of the instrument. • Lift up the reagent cover and remove the bottles. • Remove the plastic protection under the reagents. •...
  • Page 311 Sensors check and adjustment Procedure RAS356B Sensors check and adjustment • Concerns Sensors check and adjustment • Required tools Allen keys • Required products None • Intervention time 2 hours • Frequency On request • Specific kit or consumables Disposal gloves and white coat must be worn by the operator. Local or national regulations must be applied in all the operations.
  • Page 312 PENTRA TECHNICAL MANUAL SENSORS CHECK AND ADJUSTMENT PROCEDURE 1. Sensor check: Enter Menu: Service/ Super User Menu / Mechanical / Check Sensors (See “Check sensors screen”, page 2). Diag.1 Check sensors screen 1.1. Loading area: Remove Left Front Cover (See RAS342) to have acces to switches (Front cover status becomes red on previous screen).
  • Page 313 PROCEDURE RAS356B Diag.3 Stop rack loading sensor right 1.2. Sampler Transfer Rail: Tube Height Control: Push on sensor lever and check that Tube Height Control status becomes red (See “Tube heigt control”, page 3). PUSH Diag.4Tube heigt control Tube Sensor: Push on sensor lever and check that Tube Sensor status becomes red (See “Tube sensor”,...
  • Page 314 PENTRA TECHNICAL MANUAL SENSORS CHECK AND ADJUSTMENT PROCEDURE and right rack lockers”, page 4). RACK Diag.6 Left and right rack lockers 1.3. Unloading area: Rack End Transfer: Push a rack in the rail until it comes in mechanical stop against unloading tray and check that Rack End Transfer status becomes red (See “Rack end transfer”, page 4).
  • Page 315 PROCEDURE RAS356B 1.4. Covers: Front cover: Open Right Front Cover and check that Front Cover status becomes red then close it and repeat this operation with Left Front Cover (See “Front cover”, page 5). Diag.9 Front cover Thermic Panel: Open Thermic Panel and ckeck that Thermic Panel status becomes red (See “Thermic panel”, page 5).
  • Page 316 PENTRA TECHNICAL MANUAL SENSORS CHECK AND ADJUSTMENT PROCEDURE 2. Sensors Adjustment 2.1. Loading area: Stop Rack Loading Sensor Left (reference:XDA741AS). Stop Rack Loading Sensor Right (references: XDA742AS). Adjustment of this two sensors is the same. This sensors are delivered on bracket and are prestressed.
  • Page 317 PROCEDURE RAS356B Bracket mechanical stop position Detection position 0.5 mm Bracket Diag.14 Bracket / switch adjustment 2.2. Sampler Transfer Rail: Tube Height Control (reference: CAE10A). Do not touch the TH M3x16 screw and HU M3 nut. Unscrew the two CHC M2x10 screws and adjust switch to have detection in this position (See “Tube height control”, page 7) and no detection when you put a 0.3 mm gauge between TH M3x16 screw and the plate (See...
  • Page 318 PENTRA TECHNICAL MANUAL SENSORS CHECK AND ADJUSTMENT PROCEDURE Left Rack Locker / Right Rack Locker (reference: CAE010A). Enter: Menu\Service\Technician Menu\Gains\Sampler Adjustment Put the rack on Loading Tray. Press Check Rack Transfer Movement button. Rack moves under Tracers (followers), then it run 25 motions on the left and 25 on the right. The following screen shows the detection (A) and non-detection (B) zones of the Tracer switches (See “Tracers detection...
  • Page 319 PROCEDURE RAS356B 2.3. Unloading area: Rack End Transfer (reference: CAE011A). Put a 0.5 mm gauge against Ejection Tray mechanical stop, then push a rack against this gauge. Loseen the two CHC M2x10 screws and adjust Rack End Transfer switch to have detection in this position, then screw the switch (See “Rack end transfer adjustment”, page 9).
  • Page 320 PENTRA TECHNICAL MANUAL SENSORS CHECK AND ADJUSTMENT PROCEDURE 2.5. Others: Tube holder cam (reference: CAE006A) Open tube holder (completely) and adjust the wheel of the left switch in the «V» of the left cam, then screw the switches and the left cam. Closed the Tube Holder and adjust the right cam to have the wheel of the right switch in the «V»...
  • Page 321 Save and restore settings RAS357B Save and restore settings • Concerns • Workstation setting save and restore • Analyzer setting save and restore • Analyzer settings printing • Required tools Floppy disk • Required products None • Intervention time 30 min •...
  • Page 322 PENTRA TECHNICAL MANUAL PROCEDURE For analyser, the followings data are saved: Cycles counter, Reference CTN values,Temperature recorded,Calibration coefficient, Motor Home and Motion Values, Needle Tube Holder Position, Window Counting and Reagent Levels. For workstation, the followings data are saved: Types, alarms level, Global Variables, limits level, pathological levels and the version number. 1.
  • Page 323 SAVE AND RESTORE SETTINGS RAS357B If floppy disk is not formated, it will be automatically formated. 1.2. Analyser setting restore Click on «Restore Setting From Hard Disk» button to restore analyser setting from Hard disk (See Diag.1 “Save restore settings screen”, page 2).
  • Page 324 PENTRA TECHNICAL MANUAL PROCEDURE 3. Print Enter: Menu / Settings Press the Print/Send button (A), the following window appears (See Diag.7 “Settings print window”, page 4). Diag.7Settings print window Select an option then press the «Validate» button. 3.1. Print analyzer settings This option allows to print: - Mechanical Systems \ Technical adjustment parameters - Pulse Adjustment parameters...
  • Page 325 Exploded views Section 8: Exploded views 1. Valve 1 to 12............8-3 2. Valves 13 to 19 ............8-4 3. Valves 20 to 23 ............8-5 4. Valves 24 to 30 ............8-6 5. Valves 31 to 35 ............8-7 6. Reagents syringe assy ..........8-8 7.
  • Page 326 23. 5DIFF syringe motor assy........8-25 24. LMNE flowcell ............8-26 25. Reagent heating coil ..........8-27 26. Chambers assy ............8-28 27. Sampling percutor motor ........8-29 28. Tube holder assy ..........8-30 29. Tube holder assy ..........8-31 30. Carriage belts ............8-32 31. Mixer assy ............8-33 32.
  • Page 369 Spare part list Section 9: Spare part list 1. Spare part list ............9-2...
  • Page 370 PENTRA TECHNICAL MANUAL RAA022B 1. Spare part list Table 1: Spare part list Part Number Designation ABC006A PCB,BACKLIGHT NEC COMPUTER P80 CAE006A SWITCH,MICROSWITCH XC5-81-82 CAE010A SWITCH,MICROSWITCH XC5-81 CAE011A SWITCH,MICROSWITCH XCH5-S2 CAE019A SWITCH,MICROSWITCH XCC5-81-S4 CAE020A SWITCH,MICROSWITCH XGG2A CAY013AS PCB,LCD COLOR SCREEN P80 CBC008A PCB,BARCODE INTERNAL P80 CBK043A...
  • Page 371 SECTION 09 SPARE PARTS LIST Table 1: Spare part list Part Number Designation FAA054A O’RING,SAMPL. NEEDLE C+/P80 FAA057A O’RING,PIERC NEEDLE P120/C+/80 FAA064A O’RING,SAMPL. SYRINGE P60/P80 FAA065A O’RING,REAGENT SYRINGE D=6,3 FAA066A O’RING,DRAINING CHAMBER P60/80 FAA067A O’RING,5DIFF SYRINGE D=2,4 FAJ008A COVER,CLOSING MAGNET P80 FAK001A CHAMBER,APERTURE 50µ...
  • Page 372 PENTRA TECHNICAL MANUAL RAA022B Table 1: Spare part list Part Number Designation GBL0215 CUP,OVERFLOW TOP MOTOR PCB P80 GBL0216 CUP,OVERFLOW MOTOR BOARD P80 GBL0254S SAMPLING,OPT. TUBE HOLDER P80 GBL0280 RACK,10 TUBES 13x82 P80 HAE026B STICKER,VALVES JOUCO (1-10) HAE027B STICKER,VALVES JOUCO (11-20) HAE028B STICKER,VALVES JOUCO (21-30) HAE029B...
  • Page 373 SECTION 09 SPARE PARTS LIST Table 1: Spare part list Part Number Designation XBA478A MOTOR,CARRIAGE P80 XBA479A MOTOR,BLOCK FOR SAMPL. SYR. P80 XBA490A CABLE,LMNE FLOWCEL CATHOD WIRE XBA492A FITTING,GROUND FOR REAGENT P80 XBA507AS FAN,FOR COMPUTER P80 XCA166A CHAMBER,ISOLATOR (LONG) XCA167A CHAMBER,ISOLATOR (SMALL) XCA191A CHAMBER,ISOLATOR VENT.
  • Page 374 PENTRA TECHNICAL MANUAL RAA022B Table 1: Spare part list Part Number Designation XDA790AS SAMPLING,RACK TRACTOR ASSY P80 XDA791AS SAMPLING,RIGHT RACK LOADER P80 XDA792AS SAMPLING,LEFT RACK LOADER P80 XDA793AS BELT,RACK LOADING P80 XDA794AS SAMPLING,TUBE DETECTION P80 XDA795AS SAMPLING,RACK TRACER ASSY P80 XDA796AS SAMPLING,RACK LOADING LOCK P80 XDA797AS...

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