Summary of Contents for Dionex UltiMate 3000 LCi
- Page 1 ® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides January 2010 Rev. 02 © 2010 Dionex...
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Page 3: Table Of Contents
Scope of Delivery ..................... 29 6.2.1 Automated Method Scouting ................29 6.2.2 Extension Kit for Automated Method Scouting (P/N 6040.0100) ....... 30 Stacking the Modules ....................31 6.3.1 Single-stack without Extension Kit ..............31 January 2010 Rev. 02 Page i © 2010 Dionex... - Page 4 Quick Installation Guides 6.3.2 Dual-stack with Extension Kit ................32 Flow Schematics ...................... 33 6.4.1 Single-stack without Extension Kit ..............33 6.4.2 Dual-stack with Extension Kit ................35 Parts ............................37 Page ii Rev. 02 January 2010 © 2010 Dionex...
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Page 5: Introduction
This document is believed to be complete and accurate at the time of publication. In no event shall Dionex be liable for incidental or consequential damages in connection with or arising from the use of this document. -
Page 6: Definition Of Lci
1.3 Typical System Setups 1.3.1 Single-Stack In many cases, bench space is limited; hence the Dionex UltiMate 3000 systems are designed to consume as little space as possible. We assume that a single-stack setup is the most common case; therefore, the LCi capillary kits have been optimized for this scenario. -
Page 7: Dual-Stack
Even though Viper withstands UHPLC backpressures of up to 1,200 bar (17,400 psi), it is a finger tight fitting system, which requires only small torques to seal. Therefore, it is essential to obey the following guidelines to avoid damages by over-tightening. January 2010 Rev. 02 Page 3 © 2010 Dionex... - Page 8 Opening and closing Viper connections at high system pressures may reduce the life- time of the fitting system. If you are unable to get a Viper connection tight, call your local Dionex representative or replace it with a new capillary.
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Page 9: Viper Capillary Kits And Packaging
In addition, dedicated Viper capillary RS and SD kits containing 3 capillaries each are available to equip Dionex UltiMate 3000 systems with Viper. Figure 6: Packaging box for Viper RS and SD capillary kits for UltiMate 3000 systems (single system, see Figure 1). - Page 10 Viper capillary (SST), ID x L 0.18 mm/0.005” x 350 mm 1 pc. Viper capillary (SST), ID x L 0.18 mm/0.005” x 250 mm 1 pc. Viper Installation and Operation Guide, short version 1 pc. Page 6 Rev. 02 January 2010 © 2010 Dionex...
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Page 11: Lci Capillary Kits
Independent of the ordered kit, you will always receive the same box, accommodating all necessary accessories and capillaries for your individual LCi kit. Figure 7: Packaging box for all Viper LCi capillary kits. January 2010 Rev. 02 Page 7 © 2010 Dionex... -
Page 12: Viper Labeling
1/16” and 1/32” OD capillaries. In addition, a set of 5 blank clips and stickers is supplied for individual labeling purposes. Figure 8: Removable labeling clip with sticker for individual capillary labeling. Page 8 Rev. 02 January 2010 © 2010 Dionex... -
Page 13: General System Preparation
If necessary, cut the tubing with a sharp knife or blade. Use only the eluent lines shipped with the solvent rack. 4. Place the complete assembly in the solvent reservoir. 5. Hand-tighten the solvent reservoir caps. January 2010 Rev. 02 Page 9 © 2010 Dionex... - Page 14 Therefore, exchange the solvents at regular intervals. Rinse the bottles thoroughly with fresh eluent before reusing them. Replace the frits (P/N 6268.0110), as necessary. Page 10 Rev. 02 January 2010 © 2010 Dionex...
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Page 15: Using 10-Port Instead Of 6-Port Tcc-3X00 Valves
The 6-port valve has a direct fluidic connection between port 1 and port 6. To achieve this fluidic connection for a 10-port valve, Dionex recommends connecting ports 7 and 10 with an additional suitable capillary. The inner diameter of this capillary should be 0.13 mm (0.005″) for a rapid separation (RS) setup or 0.18 mm (0.007″) for a standard (SD) setup. - Page 16 ® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides Page 12 Rev. 02 January 2010 © 2010 Dionex...
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Page 17: Viper Tandem Operation Capillary Kits, Dual-Ternary Standard (P/N 6040.2804) Or Rapid Separation (P/N 6040.2803)
2-position 10-port valve for column switching, a split loop well-plate autosampler, and a stackable Dionex detector, all of which are fully controlled by the Chromeleon Chromatography Data System. Prior to installation, please consider chapter 1 for important additional information. -
Page 18: Stacking The Modules
2.3 Stacking the Modules Please stack the modules as shown below. The picture is a schematic to illustrate the course of the flexible Viper capillaries in general. Dionex recommends installing all capillaries inside the modules as needed. SRD-3600 Solvent Rack and Degasser... -
Page 19: Flow Schematic
RS: 0.13 mm/0.005” x 550 mm SD: 0.18 mm/0.007” x 550 mm TCC 10-port valve right, port 1 – Detector inlet RS: 0.13 mm/0.005” x 550 mm SD: 0.18 mm/0.007” x 550 mm January 2010 Rev. 02 Page 15 © 2010 Dionex... - Page 20 In case of a dual-stack setup, the TCC 10-port valve right turns into a TCC 10-port valve left. Both stacks should have a distance of about 7 cm (2.8 inches). Connecting a post-column cooler with a stackable Dionex detector does not work without additional capillaries when setting up the system in the U-configuration (1.3.2).
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Page 21: Viper Application Switching Capillary Kits, Dual-Ternary Standard (P/N 6040.2806) Or Rapid Separation (P/N 6040.2805)
(right) and one 2-position 6-port switching valve (left) for column switching, a split loop well-plate autosampler, and a stackable Dionex detector, all of which are fully controlled by the Chromeleon Chromatography Data System. Prior to installation, please consider chapter 1 for important additional information. -
Page 22: Stacking The Modules
3.3 Stacking the Modules Please stack the modules as shown below. The picture is a schematic to illustrate the course of the flexible Viper capillaries in general. Dionex recommends installing all capillaries inside the modules as needed. SRD-3600 Solvent Rack and Degasser... -
Page 23: Flow Schematic
The figure below shows the schematic setup of the application switching configuration. Install your Viper capillaries according to the information of Table 6 and the system stack schematic ( 3.3). Figure 13: Flow schematic of the automated application switching setup January 2010 Rev. 02 Page 19 © 2010 Dionex... - Page 24 350 mm to 550 mm. Connecting a post-column cooler with a stackable Dionex detector does not work without additional capillaries when setting up the system in the U-configuration (1.3.2).
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Page 25: Viper On-Line Spe Capillary Kits, Dual-Ternary Standard (P/N 6040.2802) Or Rapid Separation (P/N 6040.2801)
2-position 6-port switching valve for column switching, a split loop well-plate autosampler, and a stackable Dionex detector, all of which are fully controlled by the Chromeleon Chromatography Data System. Prior to installation, please consider chapter 1 for important additional information. -
Page 26: Stacking The Modules
4.3 Stacking the Modules Please stack the modules as shown below. The picture is a schematic to illustrate the course of the flexible Viper capillaries in general. Dionex recommends installing all capillaries inside the modules as needed. SRD-3600 Solvent Rack and Degasser... -
Page 27: Flow Schematic
SPE column outlet – TCC 6-port valve left, port 6 RS: 0.13 mm/0.005” x 350 mm SD: 0.18 mm/0.007” x 350 mm Column outlet – Detector inlet RS: 0.13 mm/0.005” x 350 mm SD: 0.18 mm/0.007” x 350 mm January 2010 Rev. 02 Page 23 © 2010 Dionex... - Page 28 In case of a dual-stack setup, the TCC 6-port valve right turns into a TCC 6-port valve left. Both stacks should have a distance of about 7 cm (2.8 inches). Connecting a post-column cooler with a stackable Dionex detector does not work without additional capillaries when setting up the system in the U-configuration (1.3.2).
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Page 29: Viper Parallel Lc Capillary Kits, Dual-Ternary Standard (P/N 6040.2810) Or Rapid Separation (P/N 6040.2809)
2-position 6-port switching valve for column switching, a split loop well-plate autosampler, and two stackable Dionex detectors, all of which are fully controlled by the Chromeleon Chromatography Data System. Prior to installation, please consider chapter 1 for important additional information. -
Page 30: Stacking The Modules
Quick Installation Guides 5.3 Stacking the Modules Please stack the modules as shown below. The picture is a schematic to illustrate the course of the flexible Viper capillaries in general. Dionex recommends installing all capillaries inside the modules as needed. SRD-3600 Solvent... -
Page 31: Flow Schematic
Columns with a length of 200 mm or more are optimally positioned at the lower end of the TCC. Figure 15: Flow schematic of the Parallel LC setup January 2010 Rev. 02 Page 27 © 2010 Dionex... - Page 32 RS: 0.13 mm/0.005” x 350 mm SD: 0.18 mm/0.007” x 350 mm Column 2 outlet – Detector 2 inlet RS: 0.13 mm/0.005” x 350 mm SD: 0.18 mm/0.007” x 350 mm Page 28 Rev. 02 January 2010 © 2010 Dionex...
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Page 33: Viper Automated Method Scouting Kits, Quaternary Standard (P/N 6040.2808) Or Rapid Separation (P/N 6040.2807)
Viper capillary (SST), ID x L 0.18 mm/0.007” x 450 mm 1 pc. Viper union for direct connection of two Viper capillaries 7 pcs. Capillary marker 20 pcs. Labels for capillary marker 20 pcs. Capillary clip for color-coding, yellow 30 pcs. January 2010 Rev. 02 Page 29 © 2010 Dionex... -
Page 34: Extension Kit For Automated Method Scouting (P/N 6040.0100)
Eluent tubing, 0.6 m, for connecting the 10-pos 11-port valve to the LPG-3400 pump 1 pc. Laminated flow scheme for Automated Method Scouting 1 pc. Quick installation guide UltiMate 3000 Viper Capillary Kits for Intelligent LC (LCi) 1 pc. System Solutions Page 30 Rev. 02 January 2010 © 2010 Dionex... -
Page 35: Stacking The Modules
6.3.1 Single-stack without Extension Kit Please stack the modules as shown below. The picture is a schematic to illustrate the course of the flexible Viper capillaries in general. Dionex recommends installing all capillaries inside the modules as needed. SRD-3600 Solvent Rack and Degasser... -
Page 36: Dual-Stack With Extension Kit
6.3.2 Dual-stack with Extension Kit Please stack the modules as shown below. The picture is a schematic to illustrate the course of the flexible Viper capillaries in general. Dionex recommends installing all capillaries inside the modules as needed. For the use of the Method Scouting Extension Kit, a dual-stack is recommended. In combination with the second solvent rack, this setup offers space for up to 9 additional solvent bottles and the solvent selection valve to be placed on top of the system. -
Page 37: Flow Schematics
For detailed information on the installation of the solvent selection valve, please refer to the related documentation. Figure 16: Flow schematic of the Method Scouting setup without extension kit (single-stack) January 2010 Rev. 02 Page 33 © 2010 Dionex... - Page 38 Viper union together with the 150 mm Viper capillary to allow for the installation of the pre-column heater between injection valve and TCC valve right. Page 34 Rev. 02 January 2010 © 2010 Dionex...
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Page 39: Dual-Stack With Extension Kit
For detailed information on the installation of the solvent selection valve, please refer to the related documentation. Figure 17: Flow schematic of the Method Scouting setup with extension kit (dual-stack) January 2010 Rev. 02 Page 35 © 2010 Dionex... -
Page 40: 2010 Dionex
Viper unions together with 150 mm Viper capillaries. Install the pre-column heater between WPS injection valve and TCC valve right and the post-column cooler between TCC valve left and detector inlet. Page 36 Rev. 02 January 2010 © 2010 Dionex... -
Page 41: Parts
6040.2810 Viper Capillary Kit, SD System, Method Scouting, SST 6040.2808 Extension Kit for Method Scouting (RS and SD) 6040.0100 Solvent selection valve, 10-pos 11-port 6804.0020 SR-3000, Solvent Rack without Degasser 5035.9200 January 2010 Rev. 02 Page 37 © 2010 Dionex... - Page 42 Filter frit, SST, porosity 10 μm 6268.0110 Viper union for direct connection of two Viper capillaries 6040.2304 Viper plug, SST 6040.2303 Viper Capillary Kit, RS system 6040.2301 Viper Capillary Kit, SD system 6040.2302 Page 38 Rev. 02 January 2010 © 2010 Dionex...
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