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Bio-Tek FL600 Hardware Manual

Fluorescence plate reader
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BIO-TEK FL600
FLUORESCENCE
PLATE READER
HARDWARE GUIDE
29?D5;
9>CDBE=5>DC, 9>3
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Summary of Contents for Bio-Tek FL600

  • Page 1 BIO-TEK FL600 FLUORESCENCE PLATE READER HARDWARE GUIDE 29?D5; 9>CDBE=5>DC, 9>3 –...
  • Page 2 29?D5; 9>CDBE=5>DC, 9>3 – HIGHLAND PARK, BOX 998, WINOOSKI, VT 05404-0998 USA. (800) 451-5172 • (802) 655-4040 • FAX: (802) 655-7941...
  • Page 3 BIO-TEK FL600 ™ FLUORESCENCE PLATE READER For Research & Development Use Only HARDWARE GUIDE Part No. 6001000 June, 1998 Revision D...
  • Page 4 Bio-Tek Instruments, Inc. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by Bio-Tek for the use or reliability of software or equipment that is not supplied by Bio-Tek, or its affiliated dealers.

  • Page 5: Setting Up The Fl600 Reader

    Deleted Appendix A. 3/98 Preface: Added warranty. Page 2-1: Modified instructions for Setting Up the FL600 Reader. Page 5-2: Changed electrical specification for fuses. Page 5-11: Modified text and data on serial communications to reflect parity differences between KC4 reader software and FL600 diagnostic software.
  • Page 6 This Warranty is VOID if the Product has been repaired or altered by persons not authorized by Bio-Tek, or if the Product has had the serial number altered, effaced, or removed. This Warranty is VOID if any of the Products has not been connected, installed or adjusted strictly in accordance with written directions furnished by Bio-Tek.
  • Page 7 Precautions The following precautions are provided to help you avoid damaging the system: Caution: Service. The system should be serviced by Bio-Tek-authorized service personnel. Only qualified technical personnel should perform troubleshooting and service procedures on internal components.
  • Page 8 Borne de terre Erde (Bettriebserde) Borne de Tierra Terra (di funzionamento) Protective conductor terminal Borne de terre de protection Schutzleiteranscluss Borne de Tierra de Protección Terra di protezione On (Supply) Marche (alimentation) Ein (Verbindung mit dem Netz) Connectado Chiuso Bio-Tek FL600...
  • Page 9 Atención, riesgo de sacudida eléctrica Alta tensione (in questo documento Alta tensione non significa "tensione pericolosa" come definito in IEC 417) Stay clear Ne pas toucher svp. Bitte nicht berühren No tocario por favor Si prega di non toccare viii Bio-Tek FL600...
  • Page 10 Setting Up the FL600 Reader Installing KC4™ and FL600 Diagnostic Software Installing KC4 Installing the Hardware Key Installing the FL600 Diagnostic Software (Diagnostics Use)2-2 Checking the FL600 System Using the FL600 Diagnostic Software Filter Wavelength Locations Checking the COM Port...
  • Page 11 Bio-Tek FL600 Fluorescence/Absorbance Plate Reader Physical Dimensions Operating Conditions Electrical Specifications Optical Specifications Scanning Mechanism Temperature Control Performance Specifications CE Mark Approval Computer Specifications for FL600 Diagnostic Software 5-10 FL600 Serial Communications 5-11 Ordering Filters 5-12 Fluorescence Filters 5-12 Absorbance Filters...

  • Page 12: Software Requirements

    ,QWURGXFLQJ7KH)/ Thank you for selecting the Bio-Tek FL600. The FL600 is a powerful fluorescence and absorbance measurement system which measures through the use of a photomultiplier tube housed inside of a light- impermeable detection compartment. The FL600 is capable of quantifying both soluble and cell-associated fluorescence.
  • Page 13 Virtually any microplate type from 6 to 384 wells can be used with the FL600. Users can select any microplate from the FL600’s built-in list of industry-standard plates, or they can define their own unique plate format(s). The FL600 is designed to meet international radio frequency emission standards, EMC immunity standards, and user safety standards.
  • Page 14 • Color graphics adapter (VGA) • 120 MB hard drive • High-density floppy disk drive 3½" • 1 serial (COM) and 1 parallel (LPT) port • Mouse and keyboard ports • Microsoft or compatible mouse • Surge/spike protector Introduction to the Bio-Tek FL600...
  • Page 15 • 250 MB hard drive 3ULQWHU • Microsoft Windows compatible printer 6RIWZDUH5HTXLUHPHQWV • FL600 System Software, version 2 or higher • KC4™ reader control and data reduction software • MS-DOS version 5.0 or later • Microsoft Windows version 3.1 or later, or Microsoft...
  • Page 16 FL600 system software. 6HWWLQJ8S7KH)/5HDGHU CAUTION: It is recommended that two people lift the FL600, which weighs about 23 kg (50.6 lb.). Care must be taken when moving the device or removing it from the packaging. Hold the device firmly from the bottom of the case when lifting it.
  • Page 17 The FL600 and all computer hardware must be electrically grounded and connected to a viable surge protector to avoid dangerous shocks and to avoid destroying the electronic components through electrical spikes and surges. ,QVWDOOLQJ.&ŒDQG)/'LDJQRVWLF6RIWZDUH The FL600 is supplied with two Windows™ software packages, KC4™...
  • Page 18 Setup will indicate to you that all FL600 diagnostic software files will be installed into the drive and directory C:\FL600. You will be given the opportunity to change either the drive or the directory (or both). Setup creates a new group called Bio-Tek FL600. Note that this is different from the KC4™...
  • Page 19 &KHFNLQJWKH)/6\VWHP Before you begin to use the FL600 software, you should first check that the FL600 settings are correct. 8VLQJWKH)/'LDJQRVWLF6RIWZDUH Step 1: To start the FL600 diagnostic software: From within Windows, double-click on the FL600 icon. Depending on your computer speed, the FL600 diagnostic software should boot up from within 15 to 60 seconds.
  • Page 20 KC4. &KHFNLQJWKH&203RUW Next, turn on the FL600 Reader using the switch located on the right side of the front panel and check the COM Port Setting. NOTE: When the Reader has been switched on, a green light next the switch lights up and the plate transport moves out, ready to load a plate.
  • Page 21 Figure 2-4: Plate Model Information Window NOTE: You will see an error message if any of the following conditions exist: the FL600 is not correctly connected; it is not switched on; the incorrect communications port is selected; there is a cable problem. Check that...
  • Page 22 5XQQLQJ6\VWHP'LDJQRVWLFV The FL600 diagnostic software will automatically test the functionality of the Plate Reader, using the diagnostic tests. Step 1: Pull down the menu and select . You will Instrument Diagnostics see the following window. Figure 2-5: Initial FL600 Diagnostics Screen...
  • Page 23 Once the FL600 has completed its self-diagnostic tests, your screen should look similar to the one that follows: Figure 2-6: FL600 Diagnostics Screen After Testing :KDW7R'R,IWKH7HVWV)DLO If any of the diagnostic tests should fail, click on the button just Log...
  • Page 24 ([LWLQJ7KH)/'LDJQRVWLF6RIWZDUH Select Exit from the File menu. You will be prompted to save any Step 1: unsaved work. Click on Yes to save the changes and exit, No to exit without saving changes, or Cancel to forget all about exiting. Step 2: You will be prompted as to whether or not you want to close the reader door, thus parking the plate transport inside the reader.
  • Page 25 2-10 Getting Started...
  • Page 26 5HSODFLQJ)LOWHUVDQG $GMXVWLQJ3UREH+HLJKW This chapter discusses how to add and replace filters and adjust probe height for the FL600. $GGLQJDQG5HSODFLQJ)LOWHUV Adding new filters or replacing existing filter sets is a two-step process: • Changing the excitation and emission or absorption filters in the filter wheels inside the reader.
  • Page 27 Figure 3-1: Parking the Plate Transport Mechanism (Plate Carrier) Step 2: Power down the FL600, and disconnect the reader’s power cord. Never operate the instrument with the cover off. Operating the reader without a cover poses a serious shock hazard and may damage the detection circuit.
  • Page 28 (Figure 3-3). Excitation Filter Emission Filter Wheel Cover Wheel Cover Figure 3-3: Filter wheel locations Remove the filter wheel covers by pulling up on the heads of the plastic snap catches. (Figure 3-4) Bio-Tek FL600...
  • Page 29 Helpful hint: If the filter is marked 485/20X: • 485 is the center wavelength; • 20 is the bandwidth at one-half maximum transmission (BWHM) of the excitation (X) filter. Bio-Tek FL600...
  • Page 30 Fit the instrument cover, and secure it using the five screws. Seal the unused filters in a plastic container, and store them in a cool, dry location. Step 10: Re-connect the power cable, and power up the reader. The plate carrier will move to the plate load position. Bio-Tek FL600...
  • Page 31 KC4 software must match the values of the filters in the reader to avoid damaging the reader and invalidating data. NOTE 1: Settings are stored on the PC, not the FL600. Remember to reprogram the filter settings if you move the unit to another PC.
  • Page 32 8WLOL]LQJWKH)LOWHU$GDSWHU6\VWHP The FL600 comes equipped with a set of “500 series” fluorescence filters, which fit directly into the filter wheel. Should your application require absorbance filters of different wavelengths than those originally supplied with your instrument, you may order them from Bio-Tek.
  • Page 33 Changing Filters Inside the Reader. $GMXVWLQJ7RS2SWLFDO3UREH+HLJKW Each FL600 model has been factory-configured with a top probe height that will provide maximum sensitivity for the specified plate thickness. Should you wish to change plate types, it may be necessary to reset the probe height to prevent the probe from hitting the plate, or to maximize sensitivity.
  • Page 34 Check the door for ease of movement. If the door movement is impeded, try readjusting top shroud, making sure that it is on properly and that the screws are tightened sufficiently. Step 13: Attach the power cord, and turn on the power switch. The plate carrier should eject. Bio-Tek FL600...
  • Page 35 (to p v iew ) SE T SC RE W T O P PR O B E PR O B E H EIG H T A D JU ST M E N T JIG 0,&523/$7( Figure 3-6: FL600 interior configuration 3-10 Bio-Tek FL600...

  • Page 36: How To Replace The Air Filter

    Exterior maintenance consists of simply wiping the reader with a damp cloth and mild detergent. +RZWR&OHDQDQG5HSODFHWKH$LU)LOWHU Dust can affect flourescence values. The FL600 air filter should be cleaned or replaced once every six months, more often if your site is especially dusty.
  • Page 37 Step 4: If the filter is damaged or difficult to clean, or if the fan cover is damaged, replace the entire fan cover and filter with Bio-Tek P/N 49840. Step 5: Return the fan cover to its original position at the underside of the reader, and snap it back into place.
  • Page 38 Figure 4-3 Step 2: Shut off the FL600, and then unplug both the power and computer cables from the rear of the device. Bio-Tek FL600...
  • Page 39 Step 4: Remove the top cover, and set it aside. Figure 4-4 At the back of the FL600, on the left side, you will find the lamp assembly (as shown below). Excitation Filter Wheel Emission Filter Wheel...
  • Page 40 Using the 3mm Allen wrench provided in the accessories kit, unscrew the three screws mounting the lamp assembly to the lamp housing. CAUTION! Fingerprints can ruin the FL600 quartz halogen lamp and render it unusable. Step 8: Disconnect the lamp wires, and slide the lamp assembly away from the PMT base and toward the fan.
  • Page 41 Replace the reader cover, and attach it by screwing in the five screws. Step 18: Plug in the power cable and the computer cable. Step 19: Turn on the FL600. The plate transport mechanism will automatically move into the load position. WKXPE VFUHZV ODPS FRYHU ODPS 31 6 ...
  • Page 42 &KDSWHU 7HFKQLFDO,QIRUPDWLRQ This chapter contains a variety of technical information, including hardware specifications for the Bio-Tek FL600 Fluorescence Plate Reader. %LR7HN)/)OXRUHVFHQFH$EVRUEDQFH3ODWH 5HDGHU 3K\VLFDO'LPHQVLRQV Height 247 mm (97 in.) Width 707 mm (278 in.) Depth 426 mm (168 in.) Weight 23 kg (50.6 lb.) Shipping Weight 30 kg (66 lb.)
  • Page 43 (OHFWULFDO6SHFLILFDWLRQV Fuse 5.0 amp, time-delay T, glass, 5 mm x 20 mm, 250 volt-type (Part Number 46055) Input Voltage 90-132 volts AC 180-240 volts AC 47-63 Hz, 525 VA max The power supply has a auto ranging power input. Internal Voltages ±15 volt DC, +24 volt DC, +5 volt DC, -950 volt DC 2SWLFDO6SHFLILFDWLRQV...

  • Page 44: Scanning Mechanism

    1.5 mm special probe for 384-well plates with minimum crosstalk, reduced sensitivity on other plates 1.0 mm special probe for high-density media. Probe Selector Probe diameters can be different in the top read and bottom read positions. Selection of top or bottom read position is under software control.
  • Page 45 7HPSHUDWXUH&RQWURO The FL600 is fitted with a heater block which can be programmed to heat the plate to a temperature above ambient. Temperature Range Ambient +6° Celsius to 50° Celsius Uniformity Within ±0.5° Celsius across a 96 well Dynatech black plate measured with thermal probes in the center and four corner wells and 150 µl of Johnsons Baby Oil per well.
  • Page 46 Sensitivity (Specified) Bottom Reading 5.0mm Probe 10 pg/ml solution of Sodium Fluoroscein, in PBS, 150 µl per well Signal to Noise Ratio of 2 or greater. Excitation 485/20, Emission 530/25 Hellma 96-well quartz plate. 125 ng/ml solution of Propidium Iodide, in PBS, 150 µl per well Signal to Noise Ratio of 2 or greater.
  • Page 47 Up to 6 different filter sets and sensitivities per Data Capture scan; maximum of 300 scans (1800 repeat reads of the plate); wait time of up to 9999 seconds between each read of a plate 30 seconds/plate, using Rapid Mode Kinetic Speed 4-6 seconds/column (depending on computer speed), using Rapid Mode...
  • Page 48 The instrument contains 4 embedded references Fixed References which are optionally read at the start of each scan. The references are: 1. Black Acetal Reference Disk used for Optical Density 0% transmission and fluorescence background 2. Green Reference Disk for fluorescence with Excitation 485 and Emission 530nm.
  • Page 49 % D V H G R Q W K H W H V W L Q J E H O R Z  W K H G H Y L F H E H D U V W K H & ( P D U N  (OHFWURPDJQHWLF,QWHUIHUHQFHDQG6XVFHSWLELOLW\ E C E M C D I R E C T I V E 8 9 / 3 3 6 / E E C E N 5 0 0 8 1 - 1 , C L A S S A - E M I S S I O N S...
  • Page 50 C A N A D I A N D E P A R T M E N T O F C O M M U N I C A T I O N S C L A S S A This digital apparatus does not exceed Class A limits for radio emissions from digital apparatus set out in the Radio Interference Regulations of the Canadian Department of Communications.
  • Page 51 MSDOS version 5.0 or higher Bio-Tek FL600 System software version 2 Bio-Tek KC4™ reader control and data reduction software Microsoft Windows version 3.1 or higher Bio-Tek FL600 System software is compatible with Windows 95 (16 MB of RAM is recommended) Technical Information 5-10...
  • Page 52 KC4 Reader FL600 Parameter Control and Data Diagnostic Reduction Software Software Baud rate 9600 9600 Data bits Parity Even None Stop bit Note: The FL600 Diagnostic Software settings can not be changed- through either software or hardware tools. Technical Information 5-11...
  • Page 53 2UGHULQJ)LOWHUV The following list reflects standard and custom filters for use with the FL600. For the latest additions to this list, please contact Bio-Tek’s Customer Service Department. )OXRUHVFHQFH)LOWHUV These are high-quality interference filters fitted in 22 mm diameter cells. NOTE:...
  • Page 54 Wavelength/ Part FL500 use Comments Bandwidth Number (std 420-700+nm versions) 560/15 5002015 Both Alamar Blue™ Excitation Filter 560/20 5002011 Both 560/40 5002012 Both 590/20 5002025 Both Standard Filter 590/35 5002024 Both Standard Filter 620/40 5002013 Both 630/32 5002014 Both 633/10 5002017 Both 635/10...

  • Page 55: Utilizing The Filter Adapter System

    All absorbance filters are placed in the emission filter wheel and require an adapter (part #6002000). See the section entitled Utilizing the Filter Adapter System in Chapter 3, for further information. For the latest additions, please contact Bio- Tek’s Customer Service Department. FL600 ABSORBANCE FILTERS Wavelength Part Number (2874 plus wavelength)
  • Page 56 Wavelength Part Number (2874 plus Wavelength) 2874580 2874590 2874600 2874610 2874620 2874630 2874640 2874650 2874660 2874720 2874750 Technical Information 5-15...
  • Page 57 Technical Information 5-16...