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Summary of Contents for Cellular iCell
- Page 1 iCell Cardiomyocytes ® User’s Guide ...
- Page 2 CDI does not in any way guarantee or represent that you will obtain satisfactory results from using iCell Cardiomyocytes as described herein. The only warranties provided to you are included in the Limited Warranty enclosed with this guide. You assume all risk in connection with your use of iCell Cardiomyocytes.
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Page 3: Table Of Contents
Preparing 0.1% (w/v) Gelatin Solution ............... 6 Preparing the Gelatin Cell Culture Vessel ..............6 Chapter 4. Thawing Medium and iCell Cardiomyocytes ........7 Thawing iCell Cardiomyocytes Plating Medium ............7 Thawing iCell Cardiomyocytes .................. 7 ... -
Page 4: Before You Begin
Cardiomyocytes User’s Guide before handling or using ® iCell Cardiomyocytes. iCell Cardiomyocytes are for life science research use only. See Appendix A for more information and other restrictions. A Safety Data Sheet (SDS) for dimethyl sulfoxide (DMSO), in which iCell Cardiomyocytes are frozen, is available online at www.cellulardynamics.com/lit/... -
Page 5: Chapter 1. Introduction
Cardiomyocytes therefore can be maintained in culture for at least 2 weeks in the Maintenance Medium without appreciable loss of purity, enabling longer term studies. -
Page 6: Components Supplied By Cellular Dynamics
Components Supplied by Cellular Dynamics Notes Item Catalog Number iCell Cardiomyocytes CMC-100-110-001 (1 unit) CMC-100-110-005 (5 unit) CMC-100-010-000.5 (0.5 unit) CMC-100-010-001 (1 unit) CMC-100-010-005 (5 unit) CMM-100-110-001 (1 unit) iCell Cardiomyocytes Plating Medium CMM-100-110-005 (5 unit) iCell Cardiomyocytes Maintenance Medium... -
Page 7: Technical Support And Training
Notes CDI’s Technical Support Scientists have the necessary laboratory and analytical experience to respond to your inquiries. In addition, hands-on training is available (www.cellulardynamics.com/training/) or watch our Handling iCell Cardiomyocytes Training Video (www.cellulardynamics.com/cm_handling/). In-lab training may be available upon request. -
Page 8: Workflow Diagram
Culture Preparation p. 7 Plating p. 9 Replacing the Medium Culture Maintenance p. 13 Experimental Utilization Applications (native biology, drug discovery, disease modeling, functionality, etc.) Cellular Dynamics International, Inc. ... -
Page 9: Chapter 2. Handling And Storage
Handling iCell Cardiomyocytes Media iCell Cardiomyocytes Plating Medium and iCell Cardiomyocytes Maintenance Medium are shipped frozen on dry ice. Upon receipt, store iCell Cardiomyocytes media at -20°C until ready for use and at 4°C for up to 2 weeks after thaw. If media will be used for longer than 2 weeks, aliquot and freeze again after the initial thaw. -
Page 10: Chapter 3. Preparing Cell Culture Surfaces
Chapter 3. Preparing Cell Culture Surfaces Notes iCell Cardiomyocytes will plate and function on a variety of substrates including freshly prepared gelatin and fibronectin, which have been shown to support attachment, viability, and function of iCell Cardiomyocytes with similar efficiencies. -
Page 11: Chapter 4. Thawing Medium And Icell Cardiomyocytes
Complete the following steps of the thawing procedure in a time-efficient manner to facilitate optimal iCell Cardiomyocytes viability and performance. Note: Thaw no more than 3 vials of iCell Cardiomyocytes at one time. Remove the iCell Cardiomyocytes cryovial from the liquid nitrogen storage tank. - Page 12 Plating Medium to recover any residual cells from the vial. Transfer the 1 ml Plating Medium rinse from the cryovial drop-wise over 90 seconds (i.e. 1 drop every 4 - 5 seconds) to the 50 ml centrifuge tube containing the iCell Cardiomyocytes cell suspension. Gently swirl the tube while adding the medium to mix the solution completely and minimize the osmotic shock on the thawed cells.
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Page 13: Chapter 5. Plating Icell Cardiomyocytes
The near 100% purity of iCell Cardiomyocytes in combination with the minimal outgrowth of contaminating cells when cultured in iCell Cardiomyocytes Maintenance Medium (Maintenance Medium) allows highly pure cultures to be maintained for at least 2 weeks. -
Page 14: Determining Viable Cell Density
The number of viable cardiomyocytes that attach is dependent on the Plating Efficiency of the cell lot. Plating Efficiency is measured for each lot of iCell Cardiomyocytes and is found on the Certificate of Analysis provided with each ... - Page 15 Cell-based Assays The recommended plated density for performing cell-based assays in 96-well cell culture plates using iCell Cardiomyocytes is 20,000 plated cells/well. However, as mentioned in the previous section, the optimal plated density is dependent on the biology in question as well as the sensitivity of the assay and must be determined empirically.
- Page 16 Cardiomyocytes cell suspension and dispense the seeding volume of the cell suspension (i.e. 100 μl) to each well of the 96-well cell culture plate. c. Culture iCell Cardiomyocytes in a cell culture incubator at 37°C, 7% CO Media replacement and recommended washing procedures to perform before assaying cardiomyocytes are described in Chapter 6, Maintaining iCell Cardiomyocytes.
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Page 17: Chapter 6. Maintaining Icell Cardiomyocytes
See Table 2 on page 10 for recommended seeding volumes for several common cell culture vessels. Replace the Maintenance Medium every other day. Culture iCell Cardiomyocytes in a cell culture incubator at 37°C, 7% CO iCell Cardiomyocytes User’s Guide... -
Page 18: Appendices
(i) if Customer’s use of the Products is other than solely in accordance with this User’s Guide and Cellular Dynamics’ Terms and Conditions (or such other written agreement between Cellular Dynamics and Customer under which the Products are sold or transferred to Customer) or ... - Page 19 Product based on a breach of the limited warranty set forth above in this Appendix B. Any action by Customer for Cellular Dynamics’ breach of this limited warranty, for which Customer has given timely and proper notice of such breach during the Warranty Period and otherwise in accordance with this Appendix B, must be commenced by Customer within 18 months following the date of such breach.
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Page 20: Appendix C. Limited Liability
HOWEVER CAUSED AND REGARDLESS OF FORM OF ACTION WHETHER IN CONTRACT, TORT (INCLUDING NEGLIGENCE), STRICT PRODUCT LIABILITY OR OTHERWISE, EVEN IF CELLULAR DYNAMICS HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. NOTWITHSTANDING ANY OTHER TERM OR IMPLICATION TO THE CONTRARY, UNDER NO CIRCUMSTANCES SHALL CELLULAR DYNAMICS’...
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