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DATE / Rev. REVISION DESCRIPTION 01/2004-07 First edition 02/2006-08 Addition of Chapter 4.2.1 / Köhler Illumination; Update Specifications...
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The HUMAN representative shall be relieved of any liability under this warranty if the product is not used in accordance with the manufacturer's instructions, altered in any way not specified by HUMAN, not regularly maintained, used with equipment not approved by HUMAN or used for purposes for which it was not designed.
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Every effort has been made to avoid errors in text and diagrams, however, HUMAN GmbH assumes no responsibility for any errors which may appear in this publication. It is the policy of HUMAN GmbH to improve products as new techniques and components become available. HUMAN GmbH therefore has to reserve the right...
Every effort has been made to avoid errors in this text and the accompanying diagrams, however, HUMAN GmbH assumes no responsibility for any errors which may appear in this publication. It is the policy of HUMAN GmbH to improve products as new technologies and components become available. Human GmbH therefore reserves the right to change specifications if necessary in the course of such improvements.
4.2.1 Köhler Illumination The HUMASCOPE is equipped with Köhler illumination. In order to get the best image possible form the optical setup of the microscope for brightfield, phase contrast and differential interference contrast, it is crucial that the light path be set up properly.
The HUMASCOPE features coaxial coarse and fine focussing knobs on the left and right side of the instrument. The knobs are located below the stage and are easy to operate (see figure 4.0). The coarse and fine focussing range is 30mm.
User Manual Blue Filter Power Cable Dust Cover HUMAN 7 CARE AND MAINTENANCE: 1. As with any other optical instrument, the microscope should be kept in a cool, dry, dust- and acid-free location. The instrument should be covered with dust cover after use.
9 MICROSCOPE GLOSSARY Darkfield microscopy is a method of acquiring a slide image. It is specific to the way the light is projected onto a slide in the microscope equipped with darkfield condenser. The method allows elements in the field of view to be distinguished that would be invisible or poorly visible using other methods of lighting (such as brightfield or phase contrast light).
The fluorescence of a substance is seen when a molecule is exposed to a specific wavelength of light (excitation wavelength or spectrum) and the light it emits (the emission wavelength or spectrum) is of a higher wavelength. To view this fluorescence in the microscope, several light-filtering components are needed. Specific filters are needed to isolate the excitation and emission wavelengths of a fluorochrome.
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HUMAN Gesellschaft für Biochemica und Diagnostica mbH | Max-Planck-Ring 21 · 65205 Wiesbaden · Germany | Tel.: +49 61 22/99 88-0 · Fax: +49 61 22/99 88-100 | e-Mail: human@human.de · www.human.de 02/2006-08...
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