Summary of Contents for Cygnus IR-183A
- Page 1 IR-183A & IR-283A Intracellular Recording Amplifier Instruction Manual ©2002 Cygnus Technology, Inc. Cygnus Technology, Inc. P.O.Box 219 Delaware Water Gap, PA 18327 Tel: (570) 424-5701 Fax: (570) 424-5630 Email: sales@cygnustech.com web: www.cygnustech.com...
- Page 2 IR-183/IR-283, and the component parts thereof, will be free from defects in workmanship and material for a period of one year from the date of purchase. During the warranty period, Cygnus Technology, Inc. will, without charge, repair or replace, at its option, defective product or component parts.
- Page 3 3.Put a 10MegaOhm resistor between the input connector and the ground terminal. 4.Keep all switches at "off" or "Gnd" (Ground) position and turn all dials to the zero position. All dials should be at the minimum position (knobs completely counterclockwise). 5.Turn on the oscilloscope and set the gain to 10mV/div.
- Page 4 Operation: Front Panel: Connect headstage(s) and a remote switch. The headstages of the two channel model are labeled with numbers. Each amplifier channel is specifically calibrated with one headstage. It is not necessary to connect a headstage which is not in use. Rear Panel: (One set of inputs and outputs are located on the front control panel of the IR- 183.) Voltage outputs(s), I-Monitor(s) to oscilloscope, and I-Command(s) to pulse...
- Page 5 Electrode Tip Potential Adjustment 1.Ground the oscilloscope input, set it to DC mode, and adjust the beam to the center of the oscilloscope. 2. Set the input switch to "on". Adjust trace to the zero position on the screen using the Position potentiometer. The number on the position dial is the electrode tip potential.
- Page 6 Electrode Resistance: 1.Turn on the pulse generator. The pulse generator should not have any DC offset voltage. 2.Make +.5nA, 20ms pulse. (0.5 nA is equal to 50 mV at the I-Monitor.) 3.Flip the Bridge Balance switch to x1 or x5 and turn the dial until the top of the pulse is at the base line level on V-out.
- Page 7 Intracellular Bridge Balance Method If you need to readjust electrode resistance after the penetration of a cell, use the short pulse bridge balance method (See J. Neurophysiol. 41, 1662-1675, 1978.): 1.Generate a 0.5nA 1ms positive current pulse. 2.The electrode capacitance should be neutralized properly. 3.Adjust the peak potential of the charging voltage change to the baseline with the bridge balance.
- Page 8 Cell Penetration System An Audio monitor is very helpful with this procedure. Turn on Cell Penetration Switch and select mode of penetration. A. High Voltage Mode (HV Mode): This mode is most useful with very high resistance electrodes. Generally, a negative polarity is more effective , so use less intensity.
- Page 9 the resting potential begins to increase. 2) If the Resting potential begins to increase, reduce the hyperpolarizing DC current. If you are lucky, you may be able to bring the holding current all the way to zero. If not, begin the experiment anyway. 3) To protect the cell from accidental injury it is a good idea to turn off the cell penetration system when not in use.
- Page 10 Grounding The amplifier and headstage cases are connected to safety ground through the 3 prong power connector. The signal ground is floated from case ground through a 10 Ohm resistor to help eliminate ground loops. It is suggested that the preparation be grounded only through the ground jack on the headstage.
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