Agilent Technologies G1978B Setup Manual
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Agilent Technologies G1978B Setup Manual

Agilent Technologies G1978B Setup Manual

Multimode source for 6100 series single quad lc/ms
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Agilent G1978B
Multimode Source for
6100 Series Single Quad
LC/MS
Set-Up Guide
Agilent Technologies

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Summary of Contents for Agilent Technologies G1978B

  • Page 1 Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide Agilent Technologies...

  • Page 2: Safety Notices

    Notices © Agilent Technologies, Inc. 2008 No part of this manual may be reproduced in any form or by any means (including elec- tronic storage and retrieval or translation into a foreign language) without prior agree- ment and written consent from Agilent Technologies, Inc.
  • Page 3 In This Guide This guide explains how to install, maintain and troubleshoot your nanoelectospray ion source. Installation This chapter tells you how to install the multimode ion source. Verification This chapter tells you how to install the multimode ion source. Methods This chapter describes basic operation and maintenance for the multimode ion source.
  • Page 4 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide...

  • Page 5: Table Of Contents

    Verification To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive SIM Modes To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes...
  • Page 6 Contents Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide...

  • Page 7: Installation

    Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide Installation Installation Step 1. Prepare to install Step 2. Install the HV control PCA and cables Changing Sources To remove the multimode source To convert from multimode to ESI, APCI or APPI...

  • Page 8: Installation

    Installation Installation Installation This section explains how to install the G1978B multimode source on a 6100 Series Single Quad LC/MS instrument. The G1978A multimode source is not supported on these instruments. The 6100 Series Single Quad LC/MS with multimode source is supported on ChemStation B.03.01 or higher, or the ChemStation B.01.03 with a patch.

  • Page 9: Step 2. Install The Hv Control Pca And Cables

    • Valve BD-APCI supply, p/nG1960-60802 • Valve BD-APCI Needle Interlock, p/n G1960-60856 Figure 2 From left to right: G1946-80058, G1960-60802 and G1960-60856 Step 2. Install the HV control PCA and cables 1 Turn off the system power and remove the system power cord. Keep the power cord intact if the vacuum control switch box is used.
  • Page 10 Installation Step 2. Install the HV control PCA and cables Figure 4 6 Install the APCI HV power supply. The APCI HV power supply is located at the end of the AUX Module. 7 Connect ribbon cable between valve PCA and Vcap/Vchamber power supply.
  • Page 11 Figure 6 9 Connect the long ribbon cable, p/n G1960-60802, from the APCI HV power supply to the valve PCA. Figure 7 10 Insert one end of the APCI Needle Interlock cable, G1960-60856, through the slot at the front of the system and then plug it to the APCI HV connector.
  • Page 12 Installation Step 2. Install the HV control PCA and cables Figure 9 12 Close the AUX Module cover and reconnect all cables. 13 Install the multimode source to the system and connect all connectors. Figure 10 14 Put back the side, top, front and CDS cover. 15 Plug the system power cord back on and turn the front switch on.

  • Page 13: Changing Sources

    Changing Sources To remove the multimode source Do the following steps to remove the multimode source. 1 Set the source temperatures for vaporizer heater and drying gas heater to minimum values to cool the source. Use the Tune > Instrument > Edit Spray Chamber menu item to display the Edit Spray Chamber dialog box.

  • Page 14: To Convert From Multimode To Esi, Apci Or Appi

    Installation To convert from multimode to ESI, APCI or APPI 10 Place the source shipping cover on the source. To convert from multimode to ESI, APCI or APPI Never touch the source surfaces, especially when you analyze toxic substances or WA R N I N G when you use toxic solvents.

  • Page 15: To Convert From Esi, Apci Or Appi To The Multimode Source

    To convert from ESI, APCI or APPI to the multimode source If you are installing this source on this instrument for the first time, follow the steps in C A U T I O N “Installation” 1 Switch to the MSD Tune view. 2 Select Instrument/Set Spray Chamber and set all gas flows and temperatures to 0.
  • Page 16 Installation To convert from ESI, APCI or APPI to the multimode source Figure 11 10 Remove the capillary cap. If needed, moisten a clean cloth with isopropyl alcohol and wipe the capillary cap. See Figure 12 11 Place the capillary cap back on the capillary. 12 Install the new spray shield with field shaping electrodes.
  • Page 17 Figure 13 13 Screw the multimode spray shield into the holder for the spray shield. See Figure Figure 14 The field shaping electrodes should be in the nine o’clock and the six o’clock position. N O T E Loosen the end plate screws on each side to adjust the field shaping electrodes position. 14 Remove the shipping cover from the multimode source spray chamber.
  • Page 18 Installation To convert from ESI, APCI or APPI to the multimode source Figure 15 15 Install the spray chamber on the spray chamber mount. Figure 16 16 Install the nebulizer on the multimode source spray chamber. Multimode Spray Chamber I-Button Multimode source with I-Button Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide...
  • Page 19 Figure 17 17 Connect the 1/8-inch nebulizer gas tubing from the LC/MS mainframe to the nebulizer gas fitting. See Figure 18 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide To convert from ESI, APCI or APPI to the multimode source No nebulizer on top of the multimode source Figure Nebulizer with gas tubing connected...
  • Page 20 18 Connect the LC/MS sample tubing to the LC/MS diverter valve inlet filter. Figure 19 The LC/MS Liquid Chromatograph diverter valve is an integral part of the G1978B WA R N I N G safety system. The LC mobile phase flow must always be connected to the diverter valve inlet filter.

  • Page 21: Verification

    Verification To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive SIM Modes To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes...

  • Page 22: To Verify Sensitivity For 6120 Quad - G1978B Multimode, Positive Sim Modes

    Verification To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes The G6110A instrument does not support the multimode source without the Selection N O T E valve.
  • Page 23 To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes Table 1 Method name Performance Specification Sensitivity Verification for Multimode in ESI Positive SIM Mode 1 Start up the ChemStation software. 2 Change the view to MSD Tune, and start an Autotune.

  • Page 24: To Verify Sensitivity For 6130 And 6140 Quads - G1978B Multimode, Positive Sim Modes

    Verification To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive SIM Modes When the method is finished, a report prints showing the signal-to-noise ratio for the five blank and five sample peaks, and a blank-subtracted average of the sample peaks.
  • Page 25 To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive SIM Modes Table 2 Solvent Method name Performance Specification Sensitivity Verification for Multimode in ESI, Positive SIM Mode 1 Start up the ChemStation software. 2 Change the view to MSD Tune, and start an Autotune.
  • Page 26 Verification To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive SIM Modes experiments to achieve maximum instrument sensitivity 7 Review the results. When the method is finished, a report prints showing the signal-to-noise ratio for the five blank and five sample peaks, and a blank-subtracted average of the sample peaks.

  • Page 27: To Verify Sensitivity For 6130 And 6140 Quads - G1978B Multimode, Positive Scan Modes

    To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes Preparation 1 Transfer 1 mL of 5 ng/µL reserpine (Agilent G2423A) to a 50 mL volumetric flask.
  • Page 28 Verification To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes Table 3 Method name Performance Specification Sensitivity Verification for Multimode in ESI, Positive Scan Mode 1 Start the ChemStation software. 2 Change the view to MSD Tune, and start an Autotune.
  • Page 29 To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes When the method is finished, a report prints showing the signal-to-noise ratio for the five blank and five sample peaks, and a blank-subtracted average of the sample peaks. The blank-subtracted average signal-to-noise value must be greater than or equal to 20:1 peak-to-peak (100:1 RMS).

  • Page 30: To Verify Sensitivity For The G1978B Multimode Using Multiple Fia Method In Mixed Mode Operation

    Verification To verify sensitivity for the G1978B Multimode using Multiple FIA method in Mixed Mode operation To verify sensitivity for the G1978B Multimode using Multiple FIA method in Mixed Mode operation Preparation 1 Do an autotune if necessary. After the autotune has completed, you may need to wait up to 30 minutes before continuing to allow the calibrant solution to be pumped out of the Quad instrument.
  • Page 31 To verify sensitivity for the G1978B Multimode using Multiple FIA method in Mixed Mode operation d Click OK. 8 Repeat for the MMCheckSL_CI.M data file. Use the same subdirectory and change Data File to Multi_CI. 9 Repeat for the MMCheckSL_MX.M data file. Use the same subdirectory and change Data File to Multi_MX as the data file name.

  • Page 32: To Do An Autotune

    Verification To do an autotune To do an autotune Tuning the multimode source is done in MM-APCI mode only. Autotune is done from the same menu as with all sources. • From the MSD Tune view, select Instrument > Autotune menu item. The tune report will have a header with either the title MM-APCI Positive Mode - Standard Scan or MM-APCI Negative Mode - Standard Scan.

  • Page 33: Example Of Multimode Verification Report

    Verification Example of multimode verification report Example of multimode verification report Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide...
  • Page 34 Verification Example of multimode verification report Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide...

  • Page 35: Methods

    Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide Methods To setup a method to use the multimode source To create a method for positive/negative mixed mode operation To create a method for alternating ESI and APCI operation This chapter describes the tasks that you need to set up methods for the multimode source.

  • Page 36: To Setup A Method To Use The Multimode Source

    The 6100 Series Single Quad LC/MS Liquid Chromatograph diverter valve is an WA R N I N G integral part of the G1978B safety system. The LC mobile phase flow must always be connected to the diverter valve inlet filter. Never bypass the diverter valve and connect directly to the nebulizer.

  • Page 37: To Create A Method For Positive/Negative Mixed Mode Operation

    To create a method for positive/negative mixed mode operation 1 Open the MSD Spray Chamber dialog box by clicking the Instrument > Set Up MSD Signals in the Method and Run Control view. 2 Select MM-ES_APCI from the Method Spray Chamber drop-down list. 3 Check that Installed Spray Chamber is also set to MM-ES+APCI.
  • Page 38 Methods To create a method for positive/negative mixed mode operation Polarity options for Signal 1 and Signal 2 Figure 21 Positive/Negative polarity switching using Set Up MSD Signals dialog box Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide...

  • Page 39: To Create A Method For Alternating Esi And Apci Operation

    To create a method for alternating ESI and APCI operation 1 Open The MSD Spray Chamber dialog box by clicking Instrument > MSD Spray Chamber in the Method and Run Control view. 2 Set Method Spray Chamber to MM-ES+APCI. Figure 22 3 Check that Installed Spray Chamber is also set to MM-ES+APCI.
  • Page 40 Methods To create a method for alternating ESI and APCI operation 9 Click the OK button. In general, use the mixed mode operation (MM-ES+APCI setting with Signal 1) instead of N O T E alternating MM-ES (Signal 1) and MM-APCI (Signal 2) mode switching. Two times as many scans are obtained during elution of a chromatographic peak, and no delay is needed between scans, resulting in better data.
  • Page 41 Index autotune, converting from ESI, APCI or APPI, converting to ESI or APCI, diverter valve inlet filter, convert from, convert to, installation, 7, LC/MSD sample tubing, method alternating ESI and APCI, basic setup, positive/negative mixed mode, multimode nebulizer, source image, parts multimode spray shield, spray shield for multimode source,...
  • Page 42 Index Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide...
  • Page 44 In This Book This book contains installation, operation, maintenance and troubleshooting instruction for the Multimode Source for 6100 Series Single Quad LC/MS. © Agilent Technologies, Inc. 2008 Printed in USA First Edition, December 2008 *G1978-90070* G1978-90070 Agilent Technologies...

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