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G5585B PlateLoc Thermal Microplate Sealer
Quick Guide
This guide contains the following topics:
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"About this guide" on page 2
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"Safety information" on page 2
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"Getting started" on page 4
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"Sealing microplates" on page 12
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"Touchscreen menus and commands" on page 19
Original Instructions
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Summary of Contents for Agilent Technologies PlateLoc
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Page 1: Table Of Contents
G5585B PlateLoc Thermal Microplate Sealer Quick Guide This guide contains the following topics: • “About this guide” on page 2 • “Safety information” on page 2 • “Getting started” on page 4 • “Sealing microplates” on page 12 • “Touchscreen menus and commands” on page 19... -
Page 2: About This Guide
PlateLoc Sealer and you have a safety question, contact Technical Support. Safety standards The PlateLoc Sealer complies with the applicable EU Directives and bears the CE mark. See the Declaration of Conformity for compliance details. G5585B PlateLocThermal Microplate Sealer Quick Guide... - Page 3 (SPAs) and solvents, including alcohols. High-temperature hazard The hot surfaces in the PlateLoc Sealer can cause a burn injury. Do not touch the WAR N IN G PlateLoc Sealer or pry open the door while it is in operation. Use care when accessing the PlateLoc Sealer interior after the device is warmed up.
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Page 4: Getting Started
Gas-Purging PlateLoc only. Argon is an odorless, colorless, and nontoxic gas that WAR N IN G can cause rapid suffocation by displacing air. Use the Gas-Purging PlateLoc only in a well-ventilated lab area. Turn off the argon gas source when the product is not in use. - Page 5 Push the AIR ON/OFF switch to the ON position. When you turn on the air, the PlateLoc Sealer door opens. If this is the first time you are turning on the air, you must remove the packing foam inside the sealing chamber before you can use the device.
- Page 6 Push the AIR ON/OFF switch to the OFF position. Loading and unloading a roll of seal Make sure you have the supplied seal-loading card. To warm up or cool down the PlateLoc Sealer: On the touchscreen, press to display the Settings pages.
- Page 7 Press to return to the Home page. A status message appears at the top of the page, indicating that the PlateLoc Sealer is warming up or cooling down to the new set temperature. Wait for the PlateLoc Sealer to warm up or cool down to the set temperature.
- Page 8 Using scissors, cut off any wrinkled or torn material from the end of the seal so that the end of the seal has a clean, straight edge. The PlateLoc Sealer will not seal microplates properly if the seal is wrinkled or torn. C AU T I O N...
- Page 9 Push the card into the PlateLoc Sealer until it protrudes beyond the chamber doorway at the front of the device. Pull the card through the PlateLoc Sealer so that the end of the seal is past the plate stage. 00203...
- Page 10 Loading card removal While maintaining the alignment of the seal with the plate stage, press the silver close-gripper button on the back of the PlateLoc Sealer until it clicks. The red open- gripper button pops back up. Pressing the silver button closes the gripper and holds the seal in place inside the device.
- Page 11 Unloading procedure To unload a roll of seal: Power cycle the PlateLoc Sealer by turning off and turning on the device. Power cycling resets the components inside the device and removes the hold on the seal.
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Page 12: Sealing Microplates
• The sealing parameters are set correctly. • The PlateLoc Sealer is warmed up or cooled down to the sealing temperature. Standalone device procedure To load a microplate and an insert in the standalone device: Place the removable plate stage (1a) on the plate-stage support (1b), as the following figure shows. - Page 13 Sealing microplates Lab automation system procedure If the PlateLoc Sealer is installed in a lab automation system, you need to load the insert before you start the protocol run. During the run, the robot loads the microplates automatically. You do not need to manually load each microplate.
- Page 14 Sealing microplates The touchscreen automatically returns to the Home page. Status messages appear at the top of the Home page, such as, Warming up, Cooling down, or Ready. You can also monitor the following: • Seal Time displays the time remaining in the seal cycle. •...
- Page 15 Sealing microplates Selecting and editing a saved method (touchscreen) To select and edit a saved plate sealing method: On the touchscreen, press to display the Preset page. Press to move the highlight to the desired Preset row, and then do one of the following: •...
- Page 16 In the VWorks software, you set the sealing parameters when you set the Seal (PlateLoc) task properties. For details, see the VWorks Automation Control User Guide. If the PlateLoc Sealer is integrated with third-party software, see the PlateLoc Sealer ActiveX version 13.0 User Guide. Starting the seal cycle Before you set the seal cycle, make sure: •...
- Page 17 The seal cycle begins. The status bar at the top of the touchscreen displays In Progress. Gas-Purging PlateLoc only. If the Gas Purge Time is greater than 0.0 seconds, the argon fills the sealing chamber for the specified amount of time before the seal is applied.
- Page 18 If you have finished using the PlateLoc Sealer: Turn off the PlateLoc Sealer. Turn off the air at the back of the PlateLoc Sealer or at the source (house, cylinder, or pump). Lab automation system procedure You can start one or more seal cycles when you start the protocol run in the VWorks software.
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Page 19: Touchscreen Menus And Commands
To stop a seal cycle in an emergency: If the Stop button on the touchscreen is not responding fast enough, turn off the PlateLoc Sealer (1). The on/off switch is on the back of the device. Alternatively, you can disconnect the power to the PlateLoc Sealer (2). - Page 20 Agilent factory and service personnel only. Displays a keypad that authorized Agilent personnel use to display the PlateLoc service pages to set up the device and troubleshoot problems. Using the commands in the Service pages can expose you to potential injury and WAR N IN G cause equipment damage.
- Page 21 0.599–0.62 MPa (87–90 psi). Vacuum If View Vacuum Readings is set to On in the Settings page 3/5, Readings the Home page displays vacuum pressure readings (inHg) for (3 values) the following PlateLoc components: • Gripper (Gr) • Slider plate (Sl) •...
- Page 22 Default: 20 °C Range: 20.0 to 200.0 °C Gas Purge Time Available for Gas-Purging PlateLoc only. Sets the amount of time (seconds) the microplate is exposed to argon before the microplate is sealed. Press to adjust the value down or up in 0.5-second increments.
- Page 23 Touchscreen menus and commands Control or indicator Description Firmware version Displays the version of the installed firmware for the device. Settings 2/5 Control or indicator Description Screen Brightness Sets the brightness of the touchscreen display. As you reduce the brightness, the text in the touchscreen dims. To adjust the brightness, press Sleep Mode Turns on or off the Sleep Mode.
- Page 24 Touchscreen menus and commands Settings 3/5 Indicator Description Cycle Mode (200) Turns on or off the Cycle Mode. When set to On, the Cycle Mode places 200 seals on the current microplate. Note: This feature is typically used only by Agilent factory and service personnel.
- Page 25 Touchscreen menus and commands Settings 4/5 Indicator Description Gas Purge Displays No or Yes to indicate if the device is a Gas- Purging model. Small Hot Plate Displays No or Yes to indicate if the device is fitted with a small hot plate.
- Page 26 45 °C or less. Clear Seal Available only if the PlateLoc temperature is 45 °C or less. Press the button to perform the seal clearing operation. Repeat the Clear Seal operation as necessary to clear the seal.
- Page 27 Touchscreen menus and commands Preset page Control or indicator Description Preset 01 — 10 Displays the list of saved plate sealing methods that you can use. Note: By default, the recommended starting point settings for different seal and microplate types are stored as Presets 01–06.
- Page 28 Default: 20 °C Range: 20.0 to 200.0 °C Gas Purge Time Gas-Purging PlateLoc only. Sets the amount of time (seconds) the microplate is exposed to argon before the microplate is sealed. Click the left or right arrow to adjust the value down or up in 0.5-second increments.
- Page 29 In This Guide This guide summarizes the instructions in the user guide for the G5585B PlateLoc Thermal Microplate Sealer. www.agilent.com © Agilent Technologies, Inc. 2021 July 2021 *D0012111* D0012111 Revision A...