NanoEnTek ADAMII-CD34 User Manual
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NanoEnTek ADAMII-CD34 User Manual

Bench-top fluorescence cell counter
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Summary of Contents for NanoEnTek ADAMII-CD34

  • Page 1 Bench-top Fluorescence Cell Counter For ADAMII -CD34 User manual...
  • Page 2 Every effort has been made to ensure that all the information contained in the ADAMII™ User Manual is correct at the time of printing. However, NanoEntek Inc. reserves the right to make any changes necessary without notice as part of ongoing product development.

  • Page 3: Table Of Contents

    24~33 -Initial mode..............24~26 -Focusing mode.............. 27~33 Deta menu................. 34~43 Setting menu..............44~49 Cleaning & Maintenance..........Troubleshooting.............. 51~52 Error code................ 53~54 Warranty................Safety precautions............Consignes de securite............ Safety symbols..............Product specifications........... Ordering information............Technical support............ADAMII™ © 2016 NanoEntek, Inc.

  • Page 4: Introduction

    Introduction ADAMII™ is a benchtop fluorescent cell counting analyzer that can use in either a hospital or a research laboratory. It has 4-channel (Bright, Green (PE), Green (PerCP), and Blue (FITC)) light sources, and three-color fluorescence samples can analyze simultaneously. The automatic X-Y-Z stage captures a maxi- mum of 75 images and increases the accuracy of the analysis.

  • Page 5: Product Components

    ADAMII™ is shipped with the following components. Once you receive your instrument, please check that all components listed below were shipped. If any component is missed or damaged, contact your local distributor or e-mail ivdst@nanoentek.com. ADAMII™ Power cord with 4 adaptor cords USB connection cable (for U.S./Canada/ Taiwan/Japan,...

  • Page 6: Product Description

    Product description Front view Insert/Eject button Run Button State LED Insert and eject the slide from the ADAMII™. Insert/Eject button Functions as load and unload. Run button Perform all procedures of automatic counting. State LED Light up when software runs in the laptop and connection of camera and serial perform well.
  • Page 7 Power switch Serial Camera connection connection supply port port port Power supply port Power supply Power on/off Power switch Control the ADAMII™ operating Serial connection port Camera connection port Control the camera of the ADAMII™ ADAMII™ © 2016 NanoEntek, Inc.

  • Page 8: Installation

    Installation Unpacking 1. Open the box and remove the material foam in the box. 2. Carefully lift the instrument out of the box. the instrument 3. Place the instrument on a flat, level surface. Install 1. Plug the power cord into the electrical outlet and connect two USB cables. the instrument Power supply port USB port...
  • Page 9 ☞ Note: When the software runs and is connected with ADAMII™ normally, the state LED of instrument will be lighted up. CAUTION: • The instrument must turn on before the PC software can run. ADAMII™ © 2016 NanoEntek, Inc.

  • Page 10: Home Menu

    Home menu Log in At the ‘Home’ screen, enter the user ID and password to log in. Default ID and password are both ‘master’. User Click ‘User management’ when you need to manage accounts (e.g. User ID addition, password change). To create a new ID, fill out the management blank (user name, user ID, password, confirm PW) and click ‘Add’.
  • Page 11 1. Enter the User ID and set the date that you want to search. 2. Click ‘Search’ button. 3. The record of the selected user is displayed. ☞ Note: Only the master user can access the ‘’Audit trail’ . ADAMII™ © 2016 NanoEntek, Inc.
  • Page 12 Home menu Calibration 1. For calibration, go to ‘Setting’ menu and click ‘Calibrate’. procedure A pop-up window for calibration appears as shown below.
  • Page 13 3. Once again, vortex for 4 seconds and pipette for immediately. 4 sec. [Vortexing] [Pipetting Calibration Beads] 4. Load the Calibration Beads into the ADAMII Assay Slide. [Loading volume: 25 uL] ADAMII™ © 2016 NanoEntek, Inc.
  • Page 14 Home menu Calibration CAUTION: procedure • Avoid bubbles as they may negatively affect results. Warning: • Calibration Beads loading error: - Ensure that Calibration Beads is fully loaded on the ADAMII Assay Slide. - The instrument will not detect in low or high loading volumes. [Low volume] [Correct volume] 5.
  • Page 15 Home menu 7. Enter lot number of Calibration Beads. Calibration procedure 8. Select ‘Bright’ channel and adjust the focus using the focus buttons. ADAMII™ © 2016 NanoEntek, Inc.
  • Page 16 Home menu 9. Press the ‘Save BR Focus’ after adjusting focus. Calibration procedure ☞ Note: Please check ‘ADAMII™ Focus guide’ before setting the focus. [Focusing example - representative images] Bright- Good Focus Bright- Bad Focus FL- Good Focus FL- Bad Focus •...
  • Page 17 10. Select FL3 channel and adjust the focus using the focus button. procedure 11. Press the ‘Save FL Focus’ after adjusting focus. ☞ Note: Save the focus in ‘Bright’ and ‘FL3’ channel. The other channels will be automatically focused. 12. Press the ‘Run’ button. 3~4 min ADAMII™ © 2016 NanoEntek, Inc.
  • Page 18 Home menu Calibration 13. When the calibration is completed, the results will be displayed. procedure ☞ Note: If any of the results are “FAIL”, please contact the manufacturer or distributor. ☞ Note: If any of the results are “Low”, please check the focus or expiration date of the Calibration Beads.
  • Page 19 Home menu Calibration 15. The calibration results are displayed. To save the results, click ‘Save’ button. procedure 16. Press “Close” button to finish calibration. ADAMII™ © 2016 NanoEntek, Inc.

  • Page 20: Sample Loading

    Sample loading Sample Prepare a sample by checking the staining method according to the sample type. preparation ☞ Note: Please refer to the ADAMII -CD34 Kit package insert. To run a sample, click ‘Measure’ menu. Load sample Load 25 uL stained sample into the ADAMII Assay Slide.

  • Page 21: Operation

    3. Insert the Assay Slide into the slide holder. ☞ Note: Assay Slide should be inserted to the end of the slide holder, in the direction of arrow on the slide, facing up. Incomplete insertion of slide may cause erroneous results. ADAMII™ © 2016 NanoEntek, Inc.
  • Page 22 Operation Insert Warning: • Illustrations of various possible cases of erroneous Assay Slide assay slide insertion are presented below. ( O ) ( X ) Correctly inserted Not fully inserted ( X ) ( X ) Adverse direction inserted Upside down inserted (Arrow mark) 4.
  • Page 23 • Slide ejected - Off • Slide inserting and ejecting - blinking Warning: • Do not look inside the ADAMII instrument when the door is open. Direct exposure to high-energy LED light may be harmful to the eyes. ADAMII™ © 2016 NanoEntek, Inc.

  • Page 24: Measure Menu - Initial Mode, Focusing Mode

    Operation Measure menu The functions of each part in the ‘Measure’ menu are as follows. - Initial mode 1. Counting mode 2. Display area 3. Detection channel 4. Insert sample 5. Counting results area 6. Test information...
  • Page 25 (Refer to page 28) ④ Insert sample Insert the sample loaded Assay Slide and click ‘Inset sample’ button to start the test. ☞ Note: Make sure the door and slide holder cap are completely closed. ADAMII™ © 2016 NanoEntek, Inc.
  • Page 26 Operation Measure menu Counting results of the sample will be displayed. It will be activated after inserting sample. (Refer to page 33) - initial mode ⑤ Result area ⑥ Test Date, Sample/Patient ID, Antibody/Slide Lot, and Operator information will be displayed. information...

  • Page 27: Focusing Mode

    Operation Measure menu Once the Assay Slide is inserted, focusing mode will appear. - Focusing mode 1. Display area 2. Channel 3. Focus option 4. Save focus 5. Run sample 6. Eject ADAMII™ © 2016 NanoEntek, Inc.
  • Page 28 Operation Measure menu Display preview image of the sample from the camera. - Focusing mode ① Display area ② Channel Indicate which channel is displayed. For ADAMII™-CD34 assay, three fluorescent channels correspond to the markers below: • CD45 • CD34 •...
  • Page 29 2. 2ch manual focus: Set the focuses for two channels ‘Bright’ and ‘CD45’ manually, then the other two fluorescent channels will - Focusing mode ③ Focus option be automatically focused, when this option circle is selected. [Save BR focus] [Save FL3 focus] ADAMII™ © 2016 NanoEntek, Inc.
  • Page 30 Operation Measure menu [Focusing example – bright field] - Focusing mode ③ Focus option Bright- Good Focus Bright- Bad Focus #1 Bright- Bad Focus #2 Bright- Bad Focus #3 • Good Focus: Clear cell image – sharp cell membrane, dark cell body with contrast to the bright background.
  • Page 31 Viability (FITC) - Bad Focusd Focus • Good Focus: Clear cell image, bright cell body in contrast to the dark background. • Bad focus: Unclear cell image, blurry cell image and donut shaped cell image. ADAMII™ © 2016 NanoEntek, Inc.
  • Page 32 Operation Measure menu 1. Once the focusing is done, select the ‘Sample type’, ‘Dilution factor’ and number of ‘Frames’ to capture. If you want to see - Focusing mode ④ Run sample the viability, please check the Viability circle. 2. Press the “Run sample” button. 3.
  • Page 33 ☞ Note: The door should be closed when the slide holder is being ejected. If not, the door can be damaged. ☞ Note: If the door is open, the Assay Slide will not be ejected. ADAMII™ © 2016 NanoEntek, Inc.

  • Page 34: Deta Menu

    Operation Data menu Data menu provides the raw data including counting results, images from each frame in each channel. Users are able to review, save and export the data. 1. Test information 2. Sample information 3. Captured image 4. Sample volume information 5.
  • Page 35 • Sample type: Display the sample type (HPC-A, Mobilized PB, or Control) • Measured frame: Measured frame • Deleted frame: Deleted frame • Resulted frame: Final result frame (Subtract the deleted frame from the measured frame) ADAMII™ © 2016 NanoEntek, Inc.
  • Page 36 Operation Data menu Image section displays the captured images. It is also available to ③ Captured check the cells in each channel. image Press circle button of each channel to identify the cells counted through a particular channel. ADAMII™ circles the cells that were analyzed as follows.
  • Page 37 Double click the captured image, then you can check preview images ③ Captured of selected channel on the right side of the screen. To check the pre- view images of other channels, select that channel and double click image the captured image again. ADAMII™ © 2016 NanoEntek, Inc.
  • Page 38 Operation Data menu It shows the total sample volume with RBC Lysis Buffer and origi- ④ Sample nal sample volume used for staining. volume The default numerical values of total and sample volumes for each information sample type in ADAMII™-CD34 assay are shown below: •...
  • Page 39 For deleting a invalid frame, click on the right mouse button and select ‘Delete’ menu. Then, the “Result area” will show the re-calculated data, reflecting the deleted frame(s). ADAMII™ © 2016 NanoEntek, Inc.
  • Page 40 Operation Data menu How to find the invalid frames is described below: ⑦ Raw data table 1) Sample aggregation: When there is aggregation in specific area of sample, that frame should be deleted. Br image FL image 2) Dust: When there is dust inside the slide, that frame should be deleted.
  • Page 41 4) Cell debris: When there is cell debris, that frame should be deleted. ⑦ Raw data table Br image FL image 5) Overlapped circle: When there are several circles in one cell, that rame should be deleted. Br image FL image ADAMII™ © 2016 NanoEntek, Inc.
  • Page 42 Operation Data menu To review the data that is saved in ADAMII™, select the desired sample ⑧ Data list from the data list. Scroll up and down to check the data of sample. ⑨ Edit data list User can edit the data list. Please refer to below explanation of each function.
  • Page 43 • jpg file: Raw images of each channel collected for each field of view. • pdf file: A final report containing an aggregate result and calculations such as the frames, volume, event count and viability of the sample. [csv file] [jpg file] [pdf file] ADAMII™ © 2016 NanoEntek, Inc.

  • Page 44: Setting Menu

    Operation Setting menu Setting menu contains information of ADAMII™ as well as several user preferences and other Settings. It allows users to edit settings about the basic operation, software and firmware information. 1. Date & Time 2. Counting settings 3. Calibration 4.
  • Page 45 It is not recommended to adjust the sensitivity and circularity by the user. settings • Sensitivity : Refers to the contrast of the objects from the background. • Circularity : Used to determine which objects to include in the measurement based on their roundness. ADAMII™ © 2016 NanoEntek, Inc.
  • Page 46 Operation Setting menu Please refer to page 12~19 for calibration. ③ Calibration To open the calibration result list, click ‘Record’ button. You can check the data of calibration briefly including date, time, lot num- ber, user ID and result of each channel. ④...
  • Page 47 3. Find the updated software in the USB drive and double click the file to execute. 4. When you see the window below, the update completed successfully. Press OK button to proceed. 5. Program will be restarted automatically. Check that the version of software was modified properly. ADAMII™ © 2016 NanoEntek, Inc.
  • Page 48 Operation Setting menu [Firmware update] ⑥ Update 1. Save the firmware on USB drive and connect it to the PC. 2. Press ‘FW update’ button. 3. Move to the folder where ‘Firmware update file’ is located and click the file. 4.
  • Page 49 ‘Disconnected’ is displayed. ⑧ Disk info Disk info indicates available hard disk space. Please back up or delete your data from the hard drive when the free space is less than 100MB. ADAMII™ © 2016 NanoEntek, Inc.

  • Page 50: Cleaning & Maintenance

    IMPORTANT! Never disassemble or service ADAMII™ by yourself. Unauthorized repairs may damage ADAMII™ or alter its functionality, which will void your warranty. Contact ivdst@nanoentek.com or your local distributor to arrange for service. IMPORTANT! Always wipe surfaces with ethanol-soaked paper towels.

  • Page 51: Troubleshooting

    • Second, if you used the bottom of ADAMII™ CD34 Reagent Background of image is that almost used, please use the new reagent. suddenly brighten • If none of the above 2 steps solve the problem, please request technical support. ADAMII™ © 2016 NanoEntek, Inc.
  • Page 52 Troubleshooting Operation • First, vortex enough for 5 seconds and load sample immediately to read the slide again. • Second, check the focus. When the image is out of focus, the fluorescence of bead is unclear. If the focus is not set continuously in reading step, Calibration fail please request technical support.

  • Page 53: Error Code

    Reason Message Position Camera E0001 connection fail Driver Serial E0002 connection fail Serial Stage E0010 disconnected working Camera E0020 Camera disconnected. Slide holder is Slide E0030 opened. inserting Slide door is Slide E0031 opened. ejecting ADAMII™ © 2016 NanoEntek, Inc.
  • Page 54 Error code Error code Reason Message Position Data storage E0040 Data save is full Sample density Measuring E0050 is too high E0060 Update fail SW update...

  • Page 55: Warranty

    Warranty NanoEntek provides 1-year warranty service for defects of material and workmanship. If any defects occur in ADAMII™, NanoEntek provides repair services for the defective parts at its discretion. The following defects, however, are specifically excluded: 1. Defects caused by improper operation.

  • Page 56: Safety Precautions

    Safety precautions Review and follow the safety instructions below: • If water or other material enters the instrument, adaptor, or power inlet, disconnect the power cord and contact a service person. For operating environment, refer to Product Specifications. • Do not touch the main plug or power cord with wet hands. •...

  • Page 57: Consignes De Securite

    Dans la documentation de l’utilisateur, la declaration doit etre incluse pour attirer l’attention sur le fait qu’il peut y avoir des difficultes potentielles pour assurer la compatibilite electromagnetique dans d’autres environnements, en raison des perturbations rayonnees et. ADAMII™ © 2016 NanoEntek, Inc.

  • Page 58: Safety Symbols

    4.For more detailed information about disposal of your old appliance, please contact your city office, waste disposal service or visit our web-site, www.nanoentek.com. This product conforms to UL 61010-1, CAN/CSA C22.2 No.61010-1 “Safety Requirements for Electrical Equipment for Measurement, Con- trol, and Laboratory Use, Part 1: General Requirements.

  • Page 59: Product Specifications

    300 x 420 x 370 mm (L x W x H) Operating PC Generation Intel Core i5-6200U Processor ® Windows 10 (64 bit)  8 GB DDR3L1600MHz 1TB 5400rpm Hard Drive 15.6” Full HD (1920x1080) Anti Glare(16:9) WLED ADAMII™ © 2016 NanoEntek, Inc.

  • Page 60: Ordering Information

    Ordering information Components Cat. No. Description · Main instrument ADAMII , Bench-top · Power cord ADAM2 Fluorescence Cell Counter · USB connection cable CPU : 6 Generation Intel Core i5-6200U Processor ® OS : Windows 10 (64 bit)  RAM : 8 GB DDR3L1600MHz ADAMII PC, Monitor ADAM2-PC...

  • Page 61: Technical Support

    Technical support Visit the our Website at www.nanoentek.com for : · Technical resources, including manuals, FAQs, etc. · Technical support contact information · Additional product information and special offers For more information or technical assistance, please call or email. Email ivdst@nanoentek.com...
  • Page 62 851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea Tel :+82-2-6220-7942 , Fax:+82-2-6220-7999 MT Promedt Consulting GmbH Altenhofstrasse 80, 66386 St. Ingbert, Germany NanoEntek America, Inc. 220 Bear Hill Road, Suite 102, Waltham, MA 02451, USA Tel: +1-781-472-2558 , Fax: +1-781-790-5649 Email : ivdst@nanoentek.com...

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