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Summary of Contents for BIO RAD 419 Prep Cell
- Page 1 Mini Prep Cell Assembly Guide Catalog #170-2927...
- Page 2 Mini Prep Cell Assembly Guide Cast the Gel Slide the gel tube assembly through the elution chamber top and secure it on the leveled casting stand with the three screws; hand tightening is sufficient. Prepare the resolving gel solution as described in the instruction manual.
- Page 3 Mini Prep Cell Assembly Guide Assemble and Purge the Elution Chamber Soak the support frit, elution frit, and dialysis membrane in eletrophoresis buffer. Place the molded harvest ring into the lower portion of the elution chamber then press in the gray sealing gasket around the harvest ring to hold the assembly in place.
- Page 4 Mini Prep Cell Assembly Guide Gel tube assembly Elution manifold base Fig. 3 Continue purging the assembled elution chamber until elution buffer flows out through the elution buffer feedline (Figure 4). Visually inspect the elution chamber for air bubbles. If bubbles persist, tap the elution chamber against the lab bench to free the bubbles then gently push elution buffer through the assembled elution chamber until the bubbles flow out through the elution...
- Page 5 Mini Prep Cell Assembly Guide Assemble the Upper and Lower Buffer Chambers Place the assembled elution chamber/gel tube assembly into the cylindrical holding station on the casting stand. Allow the elution tube to pass out through the slot in the holding station. Slide the top of the gel tube into the grommet in the center of the upper buffer chamber.
- Page 6 Mini Prep Cell Assembly Guide Load the Sample Carefully load the sample on the surface of the gel with the 1 ml syringe and attached loading tube. Layer the sample under the electrophoresis buffer (Figure 6). Make sure the gel is not punctured with the PTFE loading tube.
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