NACALAI TESQUE COSMOSIL Quick Start Manual

For High Performance Liquid Chromatography
COSMOSIL
1. INTRODUCTION
Thank you for purchasing COSMOSIL Packed Column. To ensure maximum efficiency and long column life, we ask you to read this manual
carefully.
COSMOSIL Packed Columns are made of stainless steel and packed with specially bonded high purity spherical porous silica. The
COSMOSIL CHiRAL packed columns are for chiral separation of optical isomers.
2. CARE AND USE
1. Avoid mechanical shocks to the column.
2. Connect the column according to the flow direction indicated on the label.
3. Though the columns are made for using under 30 MPa, we recommend keeping pressure under 25 MPa.
4. We recommend keeping flow rate under 1.0 mL/min for 4.6 mmI.D. columns.
5. Keep the temperature 0 - 40°C. When mobile phase pH is over 7, keep the temperature under 25°C.
6. Wash the column with 20 - 30 ml mobile phase before connecting to the detector.
7. The product can be used in normal phase or reverse phase mode. The column is shipped in normal phase solvent. If you want to use the
column in reversed phase mode, replace the shipping solvent to ethanol or 2-propanol first as an intermediate step (they are compatible to
both normal phase and reversed phase solvents), before replacing it with reversed phase solvents.
Organic solvent
Additives
(for acidic compounds)
Additives
(for basic compounds)
pH of mobile phase
Ratio
8. If you use a mixture of aqueous and organic solvents in reversed phase mode, be careful not to cause salt precipitation. Use HPLC grade
solvent.
9. Frequent changes of the separation mode will destroy the column. One column should be used in one mode consistently. Also we
recommended keeping the chromatography conditions constant, since frequent changes of mobile phase shorten column life.
10. Pass mobile phase through membrane filter (less than 0.45 μm in pore size) before use.
11. Filter samples before injection. Avoid precipitation at injection. Using very different sample solvent from the mobile phase may cause
peak broadening, decrease resolution and reproducibility.
12. Insoluble debris from the pumping system, mobile phase, or samples trapped in the filter (2 μm) at the inlet of the column may increase
the pressure.
13. Avoid injecting air, changing flow rate rapidly. Change mobile phase at less than 1/2 of usual flow rate.
14. To maximize the column performance and to minimize the dead volume, use shorter and narrower tubing.
15. Maintain column and tubing temperature constant for good reproducibility.
16. After analysis, wash the column with shipping solvent (n-hexane / 2-propanol = 90 / 10) in normal phase use. In reversed phase use, wash
with deionized mobile phase, then replace with ethanol. Store the column tightly plugged, on vibration-free place.
17. Do not tighten the plugs and tubing too much. The ferrules could be damaged.
For information on COSMOSIL HPLC columns and our database, the COSMOSIL Application,
an incredible source of application data, please visit our web site :
http://www.nacalai.com
Contact us
®
CHiRAL PACKED COLUMNS
Normal phase mode
Alkane (n-Hexane, etc.), Alcohol (2-Propanol,
Ethanol, etc.), Tetrahydrofuran,
tert-Butylmethylethel, Chloroform, Ethyl acetate,
etc.
Trifluoroacetic acid, Formic acid, Acetic acid, etc.
normally; 0.1%, maximum; 0.5%
Diethylamine, Triethylamine, Ethylenediamine,
2-Aminoethanol, etc.
normally; 0.1%, maximum; 0.5%
2.0 - 9.0
Organic solvent / Organic solvent = any ratio
[ Japan ]
Email ： info-tech@nacalai.co.jp
TEL ： 075-211-2703
FAX ： 075-211-2673
Methanol, Acetonitrile, Ethanol, 2-Propanol,
Tetrahydrofuran, etc.
0.1%-Phosphoric acid, 0.1%-Formic acid,
20mmol/L-Phosphate buffer (pH2.5), etc.
20mmol/L-Phosphate buffer (pH7.0),
20mmol/L-Ammonium hydrogen carbonate buffer
(pH9.0 with diethylamine), etc.
2.0 - 9.0
Organic solvent / Aqueous solution = 10 / 90 - 100 / 0
1 / 2
498 Higashitamaya-cho, Nijo Karasuma,
Nakagyo-ku, Kyoto 604-0855, Japan
[ International ]
Email ： info.intl@nacalai.com
TEL ： +81-(0)75-251-1730
FAX ： +81-(0)75-251-1763
C025-1612-0
Reversed phase mode