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Summary of Contents for Omega Mag-Bind M1378-00
- Page 1 Mag-Bind® Total Pure NGS M1378-00 5 mL M1378-01 50 mL M1378-02 500 mL August 2016...
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Page 2: Table Of Contents
Mag-Bind® Total Pure NGS Table of Contents Introduction and Overview............2 Illustrated Protocol................3 Kit Contents and Preparations..........4 Storage and Stability..............4 Mag-Bind® Total Pure NGS 96-well Plate Protocol....5 Mag-Bind® Total Pure NGS 384-well Plate Protocol....8 Troubleshooting Guide.............11 Ordering Information..............12 Manual Revision: August 2016 Innovations in nucleic acid isolation... -
Page 3: Introduction And Overview
Introduction and Principle Omega Bio-tek’s Mag-Bind® Total Pure NGS Kit allows rapid and reliable isolation of PCR products with high recovery rates. The system combines Omega Bio-tek’s proprietary chemistries with the reversible nucleic acid-binding properties of magnetic beads to selectively bind DNA fragments 100 bp and larger and eliminate excess nucleotides, primers, and small, non-targeted amplification products, such as primer dimers. -
Page 4: Illustrated Protocol
Illustrated Protocol Determine the Reaction size Add Mag-Bind® Total Pure NGS and Mix Magnetize and Remove Supernatant Wash Twice with 70% Ethanol Elute DNA... -
Page 5: Kit Contents And Preparations
Kit Contents and Preparations Kit Contents Product Number M1378-00 M1378-01 M1378-02 Mag-Bind® Total Pure NGS 5 mL 50 mL 500 mL User Manual Storage and Stability Mag-Bind® Total Pure NGS is guaranteed for at least 12 months from the date of purchase when stored at 2-8°C. -
Page 6: Mag-Bind® Total Pure Ngs 96-Well Plate Protocol
Mag-Bind® Total Pure NGS - 96-well Plate Protocol Mag-Bind® Total Pure NGS Protocol for 96-well Plates Materials and Equipment to be Supplied by User: • 96-well PCR plate containing PCR samples (up to 50 μL/well) • Magnetic separation device (Recommend AlpAqua Cat# 001322). For elution volumes <30 µL AlpAqua Magnum FLX (Cat#A000400) is recommended. - Page 7 Mag-Bind® Total Pure NGS - 96-well Plate Protocol Pipet up and down 5-10 times or vortex for 30 seconds. Let sit at room temperature for 5 minutes. Place the plate on a magnetic separation device to magnetize the Mag-Bind® Total Pure NGS. Let sit at room temperature until the Mag-Bind® Total Pure NGS is completely cleared from solution.
- Page 8 Mag-Bind® Total Pure NGS - 96-well Plate Protocol 17. Let sit at room temperature for 5 minutes. 18. Place the plate on a magnetic separation device to magnetize the Mag-Bind® Total Pure NGS. Let sit at room temperature until the Mag-Bind® Total Pure NGS is completely cleared from solution.
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Page 9: Mag-Bind® Total Pure Ngs 384-Well Plate Protocol
Mag-Bind® Total Pure NGS - 384-well Plate Protocol Mag-Bind® Total Pure NGS Protocol for 384-well Plates Materials and Equipment to be Supplied by User: • 384-well PCR plate containing PCR samples (up to 100 μL/well) • Magnetic separation device for 384-well PCR plates •... - Page 10 Mag-Bind® Total Pure NGS - 384-well Plate Protocol Pipet up and down 5-10 times or vortex for 30 seconds. Let sit at room temperature for 5 minutes. Place the plate on a magnetic separation device to magnetize the Mag-Bind® Total Pure NGS. Let sit at room temperature until the Mag-Bind® Total Pure NGS is completely cleared from solution.
- Page 11 Mag-Bind® Total Pure NGS - 384-well Plate Protocol 17. Let sit at room temperature for 2-3 minutes. 18. Place the plate on a magnetic separation device to magnetize the Mag-Bind® Total Pure NGS. Let sit at room temperature until the Mag-Bind® Total Pure NGS is completely cleared from solution.
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Page 12: Troubleshooting Guide
Troubleshooting Guide Please use this guide to troubleshoot any problems that may arise. For further assistance, please contact the technical support staff, toll free, at 1-800-832-8896. Possible Problems and Suggestions Problem Cause Solution Increase the number amplification cycles Low PCR product yield for PCR Small PCR fragments normally give lower Smaller PCR product size... -
Page 13: Ordering Information
Mag-Bind® Total Pure NGS (500 mL) M1378-02 Elution Buffer (100 mL) PDR048 HiBind®, E.Z.N.A.®, Mag-Bind®, and MicroElute® are registered trademarks of Omega Bio-tek, Inc. PCR is a patented process of Hoffman-La Roche. Use of the PCR process requires a license.