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NanoSurface Cytostretcher-LV
Cell Stretching Instrument with Nanopatterned Chambers
User Manual
Last Revised 5 October 2018
(HWrCSLV2.0)
Copyright ã2018
NanoSurface Biomedical, Inc.
4000 Mason Road, Suite 304
Seattle, WA 98195
United States of America
www.nanosurfacebio.com
support@nanosurfacebio.com
+1-800-913-4403
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Table of Contents
Summary of Contents for NanoSurface Cytostretcher-LV
- Page 1 NanoSurface Cytostretcher-LV Cell Stretching Instrument with Nanopatterned Chambers User Manual Last Revised 5 October 2018 (HWrCSLV2.0) Copyright ã2018 NanoSurface Biomedical, Inc. 4000 Mason Road, Suite 304 Seattle, WA 98195 United States of America www.nanosurfacebio.com support@nanosurfacebio.com +1-800-913-4403...
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Page 2: Table Of Contents
Coating with ECM proteins without plasma treatment ................12 Chemical Modification ..........................13 SECTION 4: WARRANTY & SPECIFICATIONS ................. 14 ................................14 ARRANTY ..............................14 PECIFICATIONS APPENDIX A: INSTALLING THE FONT PACKAGE ................15 REFERENCES ............................. 16 ã 2018 NanoSurface Biomedical, Inc. NanoSurface Cytostretcher Operation Manual... -
Page 3: Introduction
Introduction Thank you for purchasing the NanoSurface Cytostretcher-LV. This device is designed to stretch NanoSurface’s proprietary in vitro culture chambers on top of an inverted optical microscope. The Cytostrecher-LV is compatible with NanoSurface Biomedical’s Environmental Control Unit (ECU). The ECU... -
Page 4: Section 1: System Overview
Section 1: System Overview A. The NanoSurface Cytostretcher-LV A. Control button: pause and resume stretch. Hold this button to turn your Cytostretcher on and off. B. AC power port and USB communication cable port. C. Offset/Pre-tension thumbscrew: allows you to pre-stretch the membrane, if desired. -
Page 5: Removing The Glass Window
(Figure A, below). After removing the bolts, gently lift the glass out with a small tool or pair of tweezers (Figure B, below). If the window is beyond cleaning or damaged, please contact support@nanosurfacebio.com ã 2018 NanoSurface Biomedical, Inc. NanoSurface Cytostretcher Operation Manual... -
Page 6: Cytostretcher Culture Chamber
C. Cytostretcher Culture Chamber Two Chamber Sizes The NanoSurface Cytostretcher is designed to work with NanoSurface’s small and medium-sized cell culture chambers. They are not compatible with the large (50 x 50 mm) chambers. Compatible chambers are available in two sizes. The small chamber has a 25 mm culture area in a square well that measures 5 x 5 mm. -
Page 7: Section 2: Using The Cytostretcher
1- Step 1: Download the software a. NanoSurface’s NaOMI software is made to work on Windows 10. For the latest software version, please contact support@nanosurfacebio.com b. The software will be a ZIP file. De-compress this. You will see two folders: one for the USB driver, and one for the Software. -
Page 8: Focus Tilt Adjustment
2. Load the chamber into the Cytostretcher-LV. Make sure you clamp the rods down with the thumbscrews. 3. Turn the Z focus adjust micrometer until the adjustment stage is at its lowest position. You should be able to look at the chamber and see that the left side is lower than the right. -
Page 9: Software Operation
D. Software Operation Overview: The Cytostretcher is controlled by the NanoSurface Operational Mechanics Interface (NaOMI). NaOMI allows you to design a particular stretch protocol and run the instrument. The instrument does not need to be connected to the computer during the actual stretch protocol, but you will need to connect to the instrument via USB in order to program it with your protocol. -
Page 10: Before Starting
Calibration: This button re-sets the zero point of the stretcher. NanoSurface recommends calibrating the instrument before each use. Note that pressing this button will actuate the stretcher. If any cells are in the device, they will be stretched to a potentially dangerous degree. -
Page 11: Example- Symmetrical Repetitive Sine Stretch With No Fixed Time: 10%, 1Hz Using A 12 Mm Chamber
(Stretch Distance / Chamber Length) X 100 = % Strain The Cytostretcher-LV is compatible with chambers that have a 5 mm length (Small chamber) or a 12 mm length (medium chamber). So, for example, applying 1.2 mm of stretch to a 12 mm chamber, you get: (1.2 mm Stretch Distance / 12 mm Chamber Length) X 100 = 10% Strain... -
Page 12: Section 3: Culturing Cells In The Cytostretcher Chambers
Incubate the solution for several hours, or overnight. This can be done at room temperature or at 37°C. After incubation, remove the solution and immediately seed your cells. Continue with your standard cell culture protocol. ã 2018 NanoSurface Biomedical, Inc. NanoSurface Cytostretcher Operation Manual... -
Page 13: Chemical Modification
. This change is also helped by elevating the temperature compared to room (25°C) . NanoSurface Biomedical, Inc. has not tested these strategies with the Cytostretcher chambers. Please see the references cited for more information. ã 2018 NanoSurface Biomedical, Inc. -
Page 14: Section 4: Warranty & Specifications
The Cytostretcher-LV is warrantied for 1 year against defects or failures in parts or workmanship. If the Cytostretcher fails or breaks within 1 year of purchase, NanoSurface will repair it at no charge. The customer is responsible for the cost of shipping the Cytostretcher to NanoSurface’s office in Seattle, WA, USA. NanoSurface will pay for return shipment of the repaired equipment. -
Page 15: Appendix A: Installing The Font Package
Installation is fast and easy. Follow these steps: 1) Locate the “NaOMI Typeface Package” folder. This is in the software installation folder that you downloaded from NanoSurface. This folder should have 13 versions of the Roboto Font. Keep this folder open. -
Page 16: References
Sui, G. et al. Solution-phase surface modification in intact poly(dimethylsiloxane) microfluidic channels. Anal. Chem. 78, 5543–5551 (2006). Wu, M. H. Simple poly(dimethylsiloxane) surface modification to control cell adhesion. Surf. Interface Anal. 41, 11–16 (2009). ã 2018 NanoSurface Biomedical, Inc. NanoSurface Cytostretcher Operation Manual...