Agilent Technologies Agilent 7700 Series ICP-MS Quick Start Manual
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Agilent Technologies Agilent 7700 Series ICP-MS Quick Start Manual

Agilent Technologies Agilent 7700 Series ICP-MS Quick Start Manual

Masshunter workstation
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Agilent 7700 Series ICP-MS
MassHunter Workstation
Quick Start Guide
This guide describes how to use the Agilent 7700 Series ICP- MS
MassHunter Workstation.
Agilent Technologies

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Summary of Contents for Agilent Technologies Agilent 7700 Series ICP-MS

  • Page 1 Agilent 7700 Series ICP-MS MassHunter Workstation Quick Start Guide This guide describes how to use the Agilent 7700 Series ICP- MS MassHunter Workstation. Agilent Technologies...

  • Page 2: Table Of Contents

    Startup Preparing for Analysis Things to check before analysis Prepare tuning solutions Ignite the plasma Check tuning Setting Quantitative Method Parameters Set up a Quantitative Method Access Method parameters directly Use the Method Wizard to create a method Running the Samples Set up the Sequence Analyze the samples Quantitative Data Analysis...

  • Page 3: Startup

    Startup To start the Agilent 7700 Series ICP- MS MassHunter Workstation software: • Double- click the ICP MS Top desktop icon , or • Select Programs > ICP- MS MassHunter Workstation > ICP- MS Top from the Windows Start menu. The ICP- MS Top window opens: Toolbar The following shortcuts are available on the ICP- MS MassHunter Workstation...

  • Page 4: Preparing For Analysis

    Preparing for Analysis The following topics will help you prepare the instrument for analysis with Agilent 7700 Series ICP- MS MassHunter Workstation. Things to check before analysis Utilities • Argon gas pressure: 500 to 700 kPa • Cell gas (Helium): 90 to 130 kPa •...

  • Page 5: Ignite The Plasma

    Ignite the plasma 1 Open the Instrument Control panel in either of the following ways: • Select Instrument Control from the Instrument menu, or • Click the Instrument Control icon. The ICP- MS Instrument Control panel opens: 2 Confirm that the instrument is in Standby mode by checking the title bar of the Instrument Control panel.

  • Page 6: Check Tuning

    Check tuning The following diagram labels the parts of the ICP- MS instrument. You can refer to this diagram during the discussion of tuning parameters in this section. Octopole Reaction System (ORS) Interface Quadrupole Mass Analyzer Skimmer Cone Octopole Ion Source Reaction Cell Lens Hyperbolic Quadrupole Rod Assembly...
  • Page 7 Toolbar The following items (shortcuts) are available on the Tuning panel toolbar: Button Action Button Action Load tuning parameters from file Save tuning parameters to file Generating tuning report Print current graph Tune sensitivity Tune Oxide Ion Tune Doubly Charged Ion Resolution and Axis Select Mode &...
  • Page 8 Operating Method When performing plasma adjustment, use a 1pbb tuning solution containing Ce. The following explains the method, refer to the online help. Settings for Preset Mode Select the nebulizer and tuning type, and set to the Preset mode. Under the Preset mode, parameters such as RF power, sampling position, carrier gas flow rate, makeup gas flow rate, and nebulizer pump speed are fixed to the preset values.
  • Page 9 Table 1 Typical Sensitivity Values (For 7700x) Mass Sensitivity (cps/ppb) No Gas Mode He Gas Mode Gas Mode (3.6ml/min) (4.0ml/min) 30,000 24,000 100,000 60,000 60,000 Oxide ratio ≤ 1.5% (≤2% for MicroFlow Nebulizer) 2 If the desired sensitivity is not achieved, select Autotune from Tume menu. Refer to the Help and execute Autotune.
  • Page 10 Reference: For 7700x The recommended tuning parameter values for 7700x are shown below. Table 2 Recommended Values for 7700x (When Using a MicroMist Nebulizer) Parameter No Gas Mode Cell Gas Mode High Energy Collision Mode Recommended Recommended Recommended Recommended Recommended Recommended Value Range...
  • Page 11 Reference: For 7700s The recommended tuning parameter values for 7700s are shown below. Table 3 Recommended Values for 7700s (When Using a MicroFlow Nebulizer) Parameter High Sensitivity Cell Gas Mode High Energy Collision Cool Plasma Mode Recom- Recom- Recom- Recom- Recom- Recom- Recom-...

  • Page 12: Setting Quantitative Method Parameters

    Setting Quantitative Method Parameters The following topics will help you create an acquisition method for quantitative analysis with the Agilent 7700 Series ICP- MS MassHunter Workstation. Set up a Quantitative Method Create a quantitative method for environmental analysis as described below: 1 Select Edit Entire Method from the Methods menu.
  • Page 13 4 On the Select Sample Types (or Select QC Items, if the Intelligent Sequence software is installed) dialog box: a Configure the list of Selected QC Items on the right to contain CalStd and Sample only. b Depending on the method you started with, you may have to use the Add- >...
  • Page 14 7 Specify the masses and element to analyze as follows: a On the Spectrum (Multi Tune) Acquisition Parameters dialog box, click the Periodic Table button as shown below: This opens the Masses dialog box shown below: G7200-90210 (Rev. A) MassHunter Workstation Quick Start Guide...
  • Page 15 b Using Table 4 on page 18 as a guide, click to select the masses to monitor for each element. Selected masses appear in red in the dialog box. Double- click on a element to open the Select Isotopes dialog box. Click the buttons for the isotopes you want to monitor.
  • Page 16 9 Set the Integration time per Point for each mass: a Click on the row for the mass in the column for the appropriate step (i.e. Step 1 for Hydrogen mode, Step 2 for Helium mode) as specified in the Reaction Gas column in the Table 4 on page 18.
  • Page 17 10 Make the following additional selections on the Spectrum (Multi Tune) Acquisition Parameters dialog box: a Select Full Quant (3) for the Peak Pattern. b Set the Repetition count to 3. c Mark the Return to First Tune Step option at the bottom of the screen, and click 11 On the Peristaltic Pump Program dialog box, set the following parameters, then click OK.
  • Page 18 Table 4 Set up information for elements and masses for Environmental Analysis Element Mass Confirmation Mode Internal Approx. Integration Mass Standard Time (sec/Point) No gas o gas 0.05 0.05 No Gas Li ( 9 or 71 0.05 9 or 71 He or (H No Gas No Gas...

  • Page 19: Access Method Parameters Directly

    Access Method parameters directly You can access some method panels directly, using the following menu items: Method parameters Window Menu Menu item Method Information ICP-MS Top Methods Edit Method Information Select Sample Items, ICP-MS Top Methods Select QC Items Select QC Items (Intelligent Sequence Software is installed) Interference Equation...
  • Page 20 Elements 4 The list of masses and elements that will be monitored is displayed. The information in this list is based on the sample and regulation selected above. Review the element information and make any changes as follows: a Right- click on the element of interest in the Element List Information. b Select one of the following options from the shortcut menu: •...

  • Page 21: Running The Samples

    Running the Samples The following topics will help you prepare a sequence, and run your samples with the Agilent 7700 Series ICP- MS MassHunter Workstation. Set up the Sequence Set up a sequence for your analysis as follows: 1 Select Edit Sample Log Table from the Sequence menu in the ICP- MS Top window.
  • Page 22 3 Fill in values for the rest of your samples as follows: a Select the first row and the subsequent rows by dragging the mouse. b Right- click in the highlighted rows and select Fill Down from the shortcut menu. c The values for Method, Type, Dil/LVL and ISTD Conc are copied from Row 1 to the selected blank rows.

  • Page 23: Analyze The Samples

    Analyze the samples 1 Prepare the following standards, samples, and each solutions to use for analysis. The solutions should contain 0.5% HCl as well as nitric acid. • Make up the following calibration standards by diluting SPEX XSTC- 760A with nitric acid to approx. 1%. The concentration of the undiluted solution is 100x that of drinking water quality standard.
  • Page 24 4 The Data Batch Directory is set to a unique name based on the current date and time. The format is year, month, day, hour, and sequential numbers. For example, 08H14K01.B is the second batch directory created between 10AM and 11AM on August 14, 2008. The parts of the name are derived as follows: 08 = 2008...

  • Page 25: Quantitative Data Analysis

    Quantitative Data Analysis The following topics will help you perform data analysis on your samples with the Agilent 7700 Series ICP- MS MassHunter Workstation. Typical scenarios for Data Analysis Description To import an existing The data was acquired with reference to a Data Analysis (DA) method. method (E.g.

  • Page 26: Open An Existing Batch For Data Analysis

    Open an existing batch for data analysis If the window is not already open, open the ICP- MS Data Analysis window in either of the following ways: • Select Main Panel from the DataAnalysis menu in the ICP- MS Top window, •...
  • Page 27 2 Import the samples as follows: a Select Import Samples from the File menu. b Navigate to the data file folder where the data files of interest are located and click the Open button. The data files in that folder are displayed on the Import Samples dialog box.

  • Page 28: Create The Data Analysis (Da) Method For Quantitative Data Analysis

    Create the Data Analysis (DA) method for Quantitative data analysis 1 Open the Method Editor window in either of the following ways: • Click the Edit Method toolbar button , or • Select Edit from the Method menu. To import existing DA method, click Import Method only or Import Method and Standard Data and select an analysis file to import.
  • Page 29 5 Select Analyte List from Method Tasks section 3 to display the Analyte List pane. To open the analyte list from the existing data: • Click the button, or • Right- click in the pane and select Load list from acquired data. You can also create an analyte list in either of the following ways: •...
  • Page 30 7 Select Full Quant from Method Tasks section 4 to display the FullQuant Method Table pane: 8 Set the following calibration curve parameters: • The calibration curve method (External Calibration / Standard Addition) • Type of VIS correction for ISTD elements •...
  • Page 31 9 The following Advanced Info method tasks are described in online help: • FullQuant Outlier • Edit Report Templates 10 Select Validate from Method Tasks section 5. Correct any method errors before proceeding. 11 Select Return to Batch- at- a- Glance from Method Tasks section 5. Click Yes to save the Data Analysis method.

  • Page 32: Process The Batch

    Process the batch 1 Initiate batch processing in either of the following ways: • Click the Process Batch toolbar button , or • Select Process Batch from the Process menu. Analysis is performed on the samples you added to the batch in the previous topic, using parameters set in the DA method.

  • Page 33: View Analysis Results

    View analysis results Batch Table 1 View the concentration and count for each element in the samples in the pane Batch Table pane. • Concentration and count for problematic data (Outlier) are displayed with a color of each Outlier background. •...

  • Page 34: Save Analysis Results

    Save analysis results Be sure to always save the analysis results in one of the following ways: As a new file: Select Save Analysis File As from the File menu. To overwrite an existing results file with the same name: •...

  • Page 35: Semi-Quantitative Analysis

    Semi-Quantitative Analysis Semi- quantitative analysis allows you to quickly scan and obtain information about the concentrations of all elements that are present in an unknown sample. It is useful when you want to obtain concentration information for a large number of elements (>70) without external calibration, such as screening prior to quantitative analysis on target elements.
  • Page 36 4 On the Select Sample Types (or Select QC Items, if the Intelligent Sequence software is installed) dialog box, set the following parameters, then click a Configure the list of Selected QC Items on the right to contain Sample, SQStd, SQBlk, and SQISTD. b Depending on the method you started with, you may have to use the Add- >...

  • Page 37: Analyze The Samples

    Analyze the samples 1 In the sequence table, set up a sequence with samples in the following order: a Tuning solution (1 ppb Li, Co, Y, Ce, Tl) - Sample Type is “SQStd” b Blank (i.e. nitric acid) - Sample Type is “SQBlk” c Sample 1 - >...
  • Page 38 Create the Data Analysis (DA) method for SemiQuant data analysis 1 Open the Method Editor window in either of the following ways: • Click the Edit Method toolbar button , or • Select Edit from the Method menu. To import existing DA method, click Import Method only or Import Method and Standard Data and select an analysis file to import.
  • Page 39 You can select elements to show in the pane by clicking Add/Remove Standard Element. 6 On the SemiQuant Method Table pane: a Mark the check box for Advanced Parameters. b Set the concentration of the elements in the standard sample being used for the SemiQuant factor correction.

  • Page 40: Process The Batch

    Process the batch 1 Initiate batch processing in either of the following ways: • Click the Process Batch toolbar button , or • Select Process Batch from the Process menu. Analysis is performed on the samples you added to the batch in the previous topic, using parameters set in the Data Analysis method, as described in “Create the Data Analysis (DA) method for SemiQuant data...

  • Page 41: Correcting The Semiquant Factors

    Correcting the Semiquant Factors On MassHunter Workstation, the SemiQuant factors are predefined for each element. The greater the SemiQuant factor, the more efficiently that element is ionized in the plasma, yielding a higher sensitivity. Use the following procedure to correct the SemiQuant factors, as necessary. 1 Select and measure standard sample containing three or four elements whose concentration are known.

  • Page 42: Change The Semiquant Factors

    Change the SemiQuant Factors You can configure the SemiQuant factor, Base mass, and Reported mass (isotopes). 1 Select SemiQuant Basic Parameters from the Global drop- down menu in the ICP- Analysis window: The SemiQuant Basic Parameters dialog box opens. 2 Make the change by either: •...

  • Page 43: Safety Notices

    Notices Technology Licenses © Agilent Technologies, Inc. 2009 C A U T I O N No part of this manual may be reproduced in The hardware and/or software described in any form or by any means (including elec- this document are furnished under a license...
  • Page 44 In this Book This Quick Start Guide presents first steps to using the Agilent 7700 Series ICP- MS MassHunter Workstation. © Agilent Technologies, Inc. 2009 Printed in USA Rev. A, June 2009 *G7200-90210* G7200-90210 Agilent Technologies...

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